MD07G1003100.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009852
Physical Location & Seq
Reverse (-)
315259 .. 321921
6663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1003100.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGACTACGAGCCCTACGATAGCAGCGGAACTGATGACGATCTTCCTCCATCACACCAGCATAGAGTTCCCAGAGGGGGCCGTGTTACGGGGAATGGAAGATCTGGTGGGGGTTCCGTCCCATATCCTAGGATGTATGGTGAAACTGACATGGAAGCACAAATTCACCAGCTTGAACGAGAAGCATACAGTTCAGTTCTGAGAGCCTTTAAAGCTCAAGCTGATGCCATTACTTGGGAAAAGGAAGCTCTGTTAACAAACCTTAGAAAGGAGTTGAGGTTATCAAATGAGGAGCACAAAGAACTTGTAGGACGGGTTAATGCAGCAGATGATGTCATAGAGAGGATAAGGGACTGGAGACAGGCTGGTGGGGTTCAACCGGGCATGCTGAGTACTGTTCAAGCAGTGCATGATCCAATACCTAGTCCTACTGTCTCTGTATCACGCAAGAAACAGAAGATGACGCAGTCAGTACATACACAGTCCTTTGCTGGGCCAACACCATCATTTCATCCACCAGCAGTTACCACATCCCACCAGCCATCTTCATCTACTGTAAAACGAGGATCTGTCCCAGCCCCAACATCAGGAGCCAAGGGCAAGAAACATAAACCCGGTCAAATTTTGCCTGGTGCTTCTTCAATGAAGCAGTTCCCTTCCTCAGGTCCAACAGGAAGGGGTCAAGTTTCGAATAGAGTTTCGACTGGTGCCGTTGTAAGTGAACCTGCTGAAGGAAAGACAAATGATACCTTGATTGGGAGGAAAGTAAGGACTAGGTGGCCCGATGACAACACTTTTTATGAAGCTGTTATAAAAGACTACAATCAAGCAGAGGGACGGCACCATCTAGTCTATGATATTAATTCGGCAAATGAAACATGGGAATGGGTTAATCTATCAGAGATATCTCCGGAGGATATTCAGTGGGTAGGTGAAGATCCTGGAATCTCTCATCGTGGGGGTTATAGTGGATCTGGTCATGGAATGAATAGATTTGTAGGCCGTGACAATGTTCCAGTTCCTGGAAGAGGTAGAGGGATCCCAAAGGGTCAAACAAGAAAAGATTTTCCGCCTTCACAGAATGGCATTGGAAAGAAGGCTCCAGACAATATCCAGTTACTTCACACAGATACTTTAATTAAGGAGGTAGAAAGGGTCTTTGGTGAAAACCGTCCAGACTCTGTTGAAATTGAGAAAGCAAAGAAAGTGTTGAAAGATCATGAACAAGCACTTATTGATGCGATTGCAAAGCTTGCTGACATTTCTGACGGTGAATGCGGACCTTTGCCTTTAAACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

434

Amino Acids

47.28

Weight (kDa)

7.29

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 53 - 122 1.9e-26 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 733
AccB1I GGYRCC 2 cut(s) 707, 840
AccB7I CCANNNNNTGG 2 cut(s) 234, 1022
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 3 cut(s) 27, 1070, 1278
AclWI GGATC 6 cut(s) 407, 574, 932, 979, 1033, 1046
AcsI RAATTY 2 cut(s) 162, 621
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 2 cut(s) 394, 474
AgsI TTSAA 6 cut(s) 176, 377, 401, 642, 1187, 1213
AjnI CCWGG 3 cut(s) 628, 940, 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 1143, 1175
AluBI AGCT 7 cut(s) 172, 215, 221, 248, 806, 1252, 1299
AluI AGCT 7 cut(s) 172, 215, 221, 248, 806, 1252, 1299
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 352, 439
AlwI GGATC 6 cut(s) 407, 574, 932, 979, 1033, 1046
AlwNI CAGNNNCTG 2 cut(s) 1022, 1181
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 4 cut(s) 79, 494, 779, 1000
ApeKI GCWGC 2 cut(s) 24, 323
ApoI RAATTY 2 cut(s) 162, 621
AseI ATTAAT 1 cut(s) 861
Asp700I GAANNNNTTC 1 cut(s) 650
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 5 cut(s) 79, 494, 665, 780, 1280
AsuC2I CCSGG 2 cut(s) 381, 615
AsuHPI GGTGA 5 cut(s) 152, 158, 944, 1175, 1283
AsuII TTCGAA 1 cut(s) 689
AvaII GGWCC 2 cut(s) 665, 1280
AvrII CCTAGG 1 cut(s) 128
AxyI CCTNAGG 1 cut(s) 661
BamHI GGATCC 1 cut(s) 1038
BanI GGYRCC 2 cut(s) 707, 840
BanII GRGCYC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 36, 335
BccI CCATC 4 cut(s) 58, 511, 550, 852
BceAI ACGGC 4 cut(s) 66, 695, 854, 987
BciT130I CCWGG 3 cut(s) 630, 942, 1023
BcnI CCSGG 2 cut(s) 381, 615
BcoDI GTCTC 2 cut(s) 352, 439
BfaI CTAG 4 cut(s) 129, 423, 774, 848
BfuAI ACCTGC 1 cut(s) 733
BglII AGATCT 1 cut(s) 101
BisI GCNGC 2 cut(s) 25, 324
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 2 cut(s) 26, 325
BmcAI AGTACT 1 cut(s) 394
Bme1390I CCNGG 5 cut(s) 381, 615, 630, 942, 1023
Bme18I GGWCC 2 cut(s) 665, 1280
BmgT120I GGNCC 5 cut(s) 79, 494, 665, 780, 1280
BmiI GGNNCC 7 cut(s) 80, 115, 592, 709, 842, 1040, 1101
BmrFI CCNGG 5 cut(s) 381, 615, 630, 942, 1023
BmsI GCATC 2 cut(s) 214, 1228
BpmI CTGGAG 2 cut(s) 376, 1086
Bpu14I TTCGAA 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 201
BpuMI CCSGG 2 cut(s) 381, 615
BsaBI GATNNNNATC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 128, 594
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bse1I ACTGG 4 cut(s) 359, 709, 1016, 1114
Bse21I CCTNAGG 1 cut(s) 661
Bse8I GATNNNNATC 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 3 cut(s) 630, 942, 1023
BseDI CCNNGG 2 cut(s) 128, 594
BseGI GGATG 3 cut(s) 138, 511, 530
BseJI GATNNNNATC 1 cut(s) 39
BseMII CTCAG 3 cut(s) 191, 380, 675
BseNI ACTGG 4 cut(s) 359, 709, 1016, 1114
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 36, 335
BseYI CCCAGC 2 cut(s) 491, 574
BshFI GGCC 4 cut(s) 81, 496, 781, 1002
BshNI GGYRCC 2 cut(s) 707, 840
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 380, 615, 911
BslFI GGGAC 4 cut(s) 104, 365, 557, 849
BsmAI GTCTC 2 cut(s) 352, 439
BsmFI GGGAC 4 cut(s) 104, 365, 557, 849
BsmI GAATGC 1 cut(s) 1280
BsnI GGCC 4 cut(s) 81, 496, 781, 1002
Bsp119I TTCGAA 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 15, 297
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
BspACI CCGC 3 cut(s) 27, 1070, 1278
BspANI GGCC 4 cut(s) 81, 496, 781, 1002
BspCNI CTCAG 3 cut(s) 192, 381, 674
BspEI TCCGGA 1 cut(s) 910
BspHI TCATGA 1 cut(s) 1219
BspLI GGNNCC 7 cut(s) 80, 115, 592, 709, 842, 1040, 1101
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 6 cut(s) 407, 574, 932, 979, 1033, 1046
BspT104I TTCGAA 1 cut(s) 689
BspT107I GGYRCC 2 cut(s) 707, 840
BsrI ACTGG 4 cut(s) 359, 709, 1016, 1114
BssECI CCNNGG 2 cut(s) 128, 594
BssMI GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
BssT1I CCWWGG 2 cut(s) 128, 594
Bst2UI CCWGG 3 cut(s) 630, 942, 1023
Bst4CI ACNGT 7 cut(s) 191, 397, 433, 483, 556, 1172, 1271
Bst6I CTCTTC 1 cut(s) 1021
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstBI TTCGAA 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 386, 1254
BstDEI CTNAG 4 cut(s) 200, 263, 389, 661
BstENI CCTNNNNNAGG 4 cut(s) 266, 660, 729, 1026
BstF5I GGATG 3 cut(s) 138, 511, 530
BstKTI GATC 8 cut(s) 43, 104, 415, 569, 940, 974, 1041, 1219
BstMAI GTCTC 2 cut(s) 352, 439
BstMBI GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
BstMWI GCNNNNNNNGC 2 cut(s) 212, 1253
BstNI CCWGG 3 cut(s) 630, 942, 1023
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 5 cut(s) 379, 613, 628, 940, 1021
BstV1I GCAGC 2 cut(s) 36, 335
BstX2I RGATCY 5 cut(s) 101, 566, 937, 971, 1038
BstYI RGATCY 5 cut(s) 101, 566, 937, 971, 1038
Bsu36I CCTNAGG 1 cut(s) 661
BsuRI GGCC 4 cut(s) 81, 496, 781, 1002
BtsCI GGATG 3 cut(s) 138, 511, 530
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 411, 929
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 2 cut(s) 386, 1254
CaiI CAGNNNCTG 2 cut(s) 1022, 1181
CciI TCATGA 1 cut(s) 1219
Cfr13I GGNCC 5 cut(s) 79, 494, 665, 780, 1280
CseI GACGC 1 cut(s) 472
Csp6I GTAC 2 cut(s) 393, 473
CviAII CATG 6 cut(s) 151, 385, 410, 879, 980, 1220
CviQI GTAC 2 cut(s) 393, 473
DdeI CTNAG 4 cut(s) 200, 263, 389, 661
DpnI GATC 8 cut(s) 42, 103, 414, 568, 939, 973, 1040, 1218
DpnII GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
DraI TTTAAA 2 cut(s) 211, 1293
Eam1104I CTCTTC 1 cut(s) 1021
EarI CTCTTC 1 cut(s) 1021
EciI GGCGGA 1 cut(s) 1059
Eco130I CCWWGG 2 cut(s) 128, 594
Eco24I GRGCYC 1 cut(s) 15
Eco32I GATATC 1 cut(s) 906
Eco47I GGWCC 2 cut(s) 665, 1280
Eco57I CTGAAG 1 cut(s) 750
Eco81I CCTNAGG 1 cut(s) 661
EcoNI CCTNNNNNAGG 4 cut(s) 266, 660, 729, 1026
EcoRII CCWGG 3 cut(s) 628, 940, 1021
EcoRV GATATC 1 cut(s) 906
EcoT14I CCWWGG 2 cut(s) 128, 594
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 2 cut(s) 128, 594
FaeI CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
FaqI GGGAC 4 cut(s) 104, 365, 557, 849
FatI CATG 6 cut(s) 150, 384, 409, 878, 979, 1219
Fnu4HI GCNGC 2 cut(s) 25, 324
FokI GGATG 3 cut(s) 145, 498, 517
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 25, 324
FspBI CTAG 4 cut(s) 129, 423, 774, 848
GluI GCNGC 2 cut(s) 25, 324
GsaI CCCAGC 2 cut(s) 495, 578
GsuI CTGGAG 2 cut(s) 376, 1086
HaeIII GGCC 4 cut(s) 81, 496, 781, 1002
HapII CCGG 3 cut(s) 380, 615, 911
HgaI GACGC 1 cut(s) 472
Hin1II CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 2 cut(s) 945, 1178
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 3 cut(s) 380, 615, 911
HphI GGTGA 5 cut(s) 152, 158, 944, 1175, 1283
Hpy166II GTNNAC 2 cut(s) 255, 722
Hpy188I TCNGA 3 cut(s) 201, 901, 1267
Hpy188III TCNNGA 5 cut(s) 588, 911, 1103, 1175, 1220
Hpy8I GTNNAC 2 cut(s) 255, 722
HpyAV CCTTC 5 cut(s) 666, 669, 725, 1083, 1090
HpyCH4III ACNGT 7 cut(s) 191, 397, 433, 483, 556, 1172, 1271
HpyCH4V TGCA 3 cut(s) 323, 409, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 1253
HpyF3I CTNAG 4 cut(s) 200, 263, 389, 661
Hsp92II CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
Kpn2I TCCGGA 1 cut(s) 910
KspAI GTTAAC 1 cut(s) 255
Kzo9I GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
LmnI GCTCC 3 cut(s) 292, 590, 1105
Lsp1109I GCAGC 2 cut(s) 36, 335
LweI GCATC 2 cut(s) 214, 1228
MaeI CTAG 4 cut(s) 129, 423, 774, 848
MaeIII GTNAC 4 cut(s) 85, 523, 1004, 1116
MalI GATC 8 cut(s) 42, 103, 414, 568, 939, 973, 1040, 1218
MboI GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
MboII GAAGA 7 cut(s) 35, 111, 469, 537, 630, 947, 1038
MflI RGATCY 5 cut(s) 101, 566, 937, 971, 1038
MhlI GDGCHC 2 cut(s) 15, 297
MluCI AATT 5 cut(s) 162, 621, 862, 1137, 1188
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 1 cut(s) 692
MroI TCCGGA 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 650
MseI TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
MslI CAYNNNNRTG 1 cut(s) 883
MspA1I CMGCKG 2 cut(s) 27, 1299
MspI CCGG 3 cut(s) 380, 615, 911
MspR9I CCNGG 5 cut(s) 381, 615, 630, 942, 1023
Mva1269I GAATGC 1 cut(s) 1280
MvaI CCWGG 3 cut(s) 630, 942, 1023
MwoI GCNNNNNNNGC 2 cut(s) 212, 1253
NciI CCSGG 2 cut(s) 381, 615
NdeII GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
NlaIII CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
NlaIV GGNNCC 7 cut(s) 80, 115, 592, 709, 842, 1040, 1101
NmuCI GTSAC 1 cut(s) 1004
NspI RCATGY 1 cut(s) 388
NspV TTCGAA 1 cut(s) 689
PacI TTAATTAA 1 cut(s) 1140
PaeI GCATGC 1 cut(s) 388
PagI TCATGA 1 cut(s) 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 1280
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 1 cut(s) 945
PflFI GACNNNGTC 1 cut(s) 466
PflMI CCANNNNNTGG 2 cut(s) 234, 1022
PfoI TCCNGGA 2 cut(s) 940, 1021
PkrI GCNGC 2 cut(s) 26, 325
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
PshBI ATTAAT 1 cut(s) 861
PsiI TTATAA 1 cut(s) 812
Psp6I CCWGG 3 cut(s) 628, 940, 1021
PspFI CCCAGC 2 cut(s) 491, 574
PspGI CCWGG 3 cut(s) 628, 940, 1021
PspN4I GGNNCC 7 cut(s) 80, 115, 592, 709, 842, 1040, 1101
PspPI GGNCC 5 cut(s) 79, 494, 665, 780, 1280
PstNI CAGNNNCTG 2 cut(s) 1022, 1181
PsuI RGATCY 5 cut(s) 101, 566, 937, 971, 1038
PsyI GACNNNGTC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 1299
RsaI GTAC 2 cut(s) 394, 474
RsaNI GTAC 2 cut(s) 393, 473
RseI CAYNNNNRTG 1 cut(s) 883
SaqAI TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
SatI GCNGC 2 cut(s) 25, 324
Sau3AI GATC 8 cut(s) 40, 101, 412, 566, 937, 971, 1038, 1216
Sau96I GGNCC 5 cut(s) 79, 494, 665, 780, 1280
ScaI AGTACT 1 cut(s) 394
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 5 cut(s) 381, 615, 630, 942, 1023
SduI GDGCHC 2 cut(s) 15, 297
SfaNI GCATC 2 cut(s) 214, 1228
SfuI TTCGAA 1 cut(s) 689
SinI GGWCC 2 cut(s) 665, 1280
SmiMI CAYNNNNRTG 1 cut(s) 883
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
SphI GCATGC 1 cut(s) 388
Sse9I AATT 5 cut(s) 162, 621, 862, 1137, 1188
SsiI CCGC 3 cut(s) 27, 1070, 1278
SspMI CTAG 4 cut(s) 129, 423, 774, 848
StyD4I CCNGG 5 cut(s) 379, 613, 628, 940, 1021
StyI CCWWGG 2 cut(s) 128, 594
TaaI ACNGT 7 cut(s) 191, 397, 433, 483, 556, 1172, 1271
TaqI TCGA 2 cut(s) 689, 701
TasI AATT 5 cut(s) 162, 621, 862, 1137, 1188
TatI WGTACW 2 cut(s) 392, 472
TfiI GAWTC 1 cut(s) 945
Tru1I TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
Tru9I TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
TscAI CASTG 2 cut(s) 411, 929
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 2 cut(s) 24, 323
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 7 cut(s) 500, 537, 659, 816, 888, 1001, 1236
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 2 cut(s) 411, 929
Tth111I GACNNNGTC 1 cut(s) 466
Van91I CCANNNNNTGG 2 cut(s) 234, 1022
VpaK11BI GGWCC 2 cut(s) 665, 1280
VspI ATTAAT 1 cut(s) 861
XagI CCTNNNNNAGG 4 cut(s) 266, 660, 729, 1026
XapI RAATTY 2 cut(s) 162, 621
XceI RCATGY 1 cut(s) 388
XmaJI CCTAGG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 4 cut(s) 129, 423, 774, 848
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.