AT3G01660

Methionine biosynthesis protein MetW

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015187
Physical Location & Seq
Forward (+)
245416 .. 246854
1439 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01660.1

Sequence Viewer

Length: 822 bp
ATGGATTCTCTTCGATTCAACCTCCAACAATCTCCATTTCAATTGAAACCTAGAATTCTCCGACCAAGAATCACCAACATATCAAACCGAATCGATGAAACCGATCCTCGACCATCATCCTCGGCCGGTAGGATCCAAAGGTTAGTACTTAACAACGAAGGTCGGACCAAACTAAACGCCGATCCTGACCGTGAATTCTATTCATATCCACGGTTCGTGAACCACGTAGATGATAGTTTCATATCCTCCTTAACCGAACTATACAGAAACCGGTTGAGACCCGGTTCGACTGTACTTGACTTGATGAGCTCATGGGTCAGCCATCTTCCAGAAGAAGTGAAGTACGAGAAAGTAGTAGGGCATGGTCTTAACGCACAGGAGCTTGCTCGTAACCCTAGACTCGATTACTTCTTCGTCAAGGATCTTAATGAAGATCAGAAGTTCGAGTTCGAAGACAAGTATTTCGACGCTGTTTTGTGTTCTGTTGGTGTACAGTATCTACAACAACCCGAAAAGGTGTTTGCAGAAGTGTATAGAGTGTTGAAACCGGGAGGAGTGTTGATAGTGAGTTTTAGCAACCGGATGTTTTATGAGAAAGCAATTAGGGTTTGGCGTGATGGAACCGAATACAGTCGGATCCAATTGGTGGTTCAGTACTTTCAGTCTATTGAAGGGTTTACTCAGCCGGAGATCATCCGGCAACAGCCTGGAGCTCAGATATCGGTTTTGACTCGGCTTATGGGTTTTATCGGTTTAGCTTCTACGCCCGATCCTTTTAATGCTGTCATTGCTTATAAAAATTTCAAACCTATTTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

273

Amino Acids

31.76

Weight (kDa)

8.51

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 124 - 187 1.2e-06 Methyltransferase domain
Methyltransf_25 PF13649 137 - 185 1e-07 Methyltransferase domain
Ubie_methyltran PF01209 145 - 193 1.6e-06 ubiE/COQ5 methyltransferase family
Methyltransf_11 PF08241 146 - 189 1.5e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01660
fragaria_vesca FvH4_6g52050
malus_domestica MD09G1020500.v1.1
prunus_persica Prupe.3G295700_v2.0.a1
pyrus_communis pycom111g01720
rosa_chinensis RchiOBHm_Chr2g0173561
rosa_laevigata RLG00000022202
rosa_multiflora Rmu_sc0002538.1_g000024 Rmu_sc0029872.1_g000002
rosa_roxburghii Rroxscaffold_2G00078780
rosa_rugosa Rorug02G0571400
rosa_samantha Rh2AG650200 Rh2BG660600 Rh2CG625500 Rh2DG675900
rosa_wichuraiana Rw2G053360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AccB7I CCANNNNNTGG 1 cut(s) 646
AclWI GGATC 8 cut(s) 98, 127, 140, 176, 429, 631, 644, 764
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 3 cut(s) 54, 194, 799
AfaI GTAC 5 cut(s) 147, 294, 344, 492, 656
AfiI CCNNNNNNNGG 1 cut(s) 646
AgeI ACCGGT 1 cut(s) 270
AgsI TTSAA 6 cut(s) 19, 41, 46, 544, 671, 805
AjnI CCWGG 1 cut(s) 706
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AluBI AGCT 4 cut(s) 309, 382, 713, 758
AluI AGCT 4 cut(s) 309, 382, 713, 758
Alw21I GWGCWC 2 cut(s) 311, 715
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 8 cut(s) 98, 127, 140, 176, 429, 631, 644, 764
AoxI GGCC 1 cut(s) 123
ApoI RAATTY 3 cut(s) 54, 194, 799
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AsiGI ACCGGT 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 165
AsuC2I CCSGG 2 cut(s) 282, 549
AsuHPI GGTGA 1 cut(s) 64
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 1 cut(s) 165
BamHI GGATCC 2 cut(s) 132, 636
BanII GRGCYC 2 cut(s) 311, 715
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 311, 715
BccI CCATC 3 cut(s) 121, 330, 611
BciT130I CCWGG 1 cut(s) 708
BcnI CCSGG 2 cut(s) 282, 549
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 2 cut(s) 51, 396
BmcAI AGTACT 2 cut(s) 147, 656
Bme1390I CCNGG 3 cut(s) 282, 549, 708
Bme18I GGWCC 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 3 cut(s) 134, 622, 638
BmrFI CCNGG 3 cut(s) 282, 549, 708
BpiI GAAGAC 1 cut(s) 459
BpmI CTGGAG 1 cut(s) 729
Bpu14I TTCGAA 1 cut(s) 450
BpuMI CCSGG 2 cut(s) 282, 549
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 120, 209
BsaWI WCCGGW 2 cut(s) 270, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse118I RCCGGY 2 cut(s) 125, 270
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 120, 209
BseGI GGATG 3 cut(s) 116, 588, 693
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 2 cut(s) 695, 728
BseRI GAGGAG 1 cut(s) 567
BseX3I CGGCCG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 125
BshTI ACCGGT 1 cut(s) 270
BshVI ATCGAT 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 311, 715
BsiSI CCGG 7 cut(s) 126, 271, 282, 548, 580, 686, 697
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 1 cut(s) 271
BsnI GGCC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 271
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 311, 715
Bsp1407I TGTACA 1 cut(s) 490
Bsp143I GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 2 cut(s) 694, 727
BspDI ATCGAT 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 134, 622, 638
BspPI GGATC 8 cut(s) 98, 127, 140, 176, 429, 631, 644, 764
BspT104I TTCGAA 1 cut(s) 450
BspTNI GGTCTC 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 2 cut(s) 125, 270
BsrGI TGTACA 1 cut(s) 490
BssAI RCCGGY 2 cut(s) 125, 270
BssECI CCNNGG 2 cut(s) 120, 209
BssMI GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 5 cut(s) 191, 213, 292, 495, 632
Bst6I CTCTTC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 681, 714
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 116, 588, 693
BstKTI GATC 8 cut(s) 106, 135, 184, 424, 436, 639, 693, 772
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 788
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 3 cut(s) 280, 547, 706
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 3 cut(s) 132, 421, 636
BstYI RGATCY 3 cut(s) 132, 421, 636
BstZI CGGCCG 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 1 cut(s) 125
BsuTUI ATCGAT 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 3 cut(s) 116, 588, 693
Cac8I GCNNGC 1 cut(s) 384
Cfr10I RCCGGY 2 cut(s) 125, 270
Cfr13I GGNCC 1 cut(s) 165
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 476
Csp6I GTAC 5 cut(s) 146, 293, 343, 491, 655
CspAI ACCGGT 1 cut(s) 270
CviAII CATG 2 cut(s) 312, 362
CviJI RGCY 9 cut(s) 125, 309, 321, 382, 685, 706, 713, 736, 758
CviKI_1 RGCY 9 cut(s) 125, 309, 321, 382, 685, 706, 713, 736, 758
CviQI GTAC 5 cut(s) 146, 293, 343, 491, 655
DdeI CTNAG 2 cut(s) 681, 714
DpnI GATC 8 cut(s) 105, 134, 183, 423, 435, 638, 692, 771
DpnII GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
EaeI YGGCCR 1 cut(s) 123
EagI CGGCCG 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Ecl136II GAGCTC 2 cut(s) 309, 713
EclXI CGGCCG 1 cut(s) 123
Eco24I GRGCYC 2 cut(s) 311, 715
Eco31I GGTCTC 1 cut(s) 271
Eco32I GATATC 1 cut(s) 720
Eco47I GGWCC 1 cut(s) 165
Eco52I CGGCCG 1 cut(s) 123
Eco53kI GAGCTC 2 cut(s) 309, 713
EcoICRI GAGCTC 2 cut(s) 309, 713
EcoRI GAATTC 2 cut(s) 54, 194
EcoRII CCWGG 1 cut(s) 706
EcoRV GATATC 1 cut(s) 720
EcoT38I GRGCYC 2 cut(s) 311, 715
FaeI CATG 2 cut(s) 315, 365
FatI CATG 2 cut(s) 311, 361
FokI GGATG 3 cut(s) 103, 595, 680
FriOI GRGCYC 2 cut(s) 311, 715
FspBI CTAG 2 cut(s) 51, 396
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 1 cut(s) 125
HapII CCGG 7 cut(s) 126, 271, 282, 548, 580, 686, 697
HgaI GACGC 1 cut(s) 476
Hin1II CATG 2 cut(s) 315, 365
HinfI GANTC 6 cut(s) 5, 15, 69, 90, 399, 730
HpaII CCGG 7 cut(s) 126, 271, 282, 548, 580, 686, 697
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 3 cut(s) 220, 491, 678
Hpy188I TCNGA 5 cut(s) 62, 165, 438, 636, 717
Hpy188III TCNNGA 3 cut(s) 185, 217, 329
Hpy8I GTNNAC 3 cut(s) 220, 491, 678
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 152, 665
HpyCH4III ACNGT 5 cut(s) 191, 213, 292, 495, 632
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 788
HpyF3I CTNAG 2 cut(s) 681, 714
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 2 cut(s) 315, 365
Kzo9I GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
LmnI GCTCC 2 cut(s) 379, 710
MaeI CTAG 2 cut(s) 51, 396
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 389
MalI GATC 8 cut(s) 105, 134, 183, 423, 435, 638, 692, 771
MboI GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
MboII GAAGA 5 cut(s) 317, 344, 403, 443, 464
MfeI CAATTG 2 cut(s) 41, 641
MflI RGATCY 3 cut(s) 132, 421, 636
MhlI GDGCHC 2 cut(s) 311, 715
MluCI AATT 6 cut(s) 41, 54, 194, 600, 641, 799
MlyI GAGTC 2 cut(s) 393, 724
MmeI TCCRAC 4 cut(s) 49, 85, 143, 614
MnlI CCTC 5 cut(s) 32, 117, 130, 256, 545
MseI TTAA 5 cut(s) 150, 251, 369, 426, 777
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 7 cut(s) 126, 271, 282, 548, 580, 686, 697
MspR9I CCNGG 3 cut(s) 282, 549, 708
MunI CAATTG 2 cut(s) 41, 641
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 1 cut(s) 788
NciI CCSGG 2 cut(s) 282, 549
NdeII GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
NlaIII CATG 2 cut(s) 315, 365
NlaIV GGNNCC 3 cut(s) 134, 622, 638
NmeAIII GCCGAG 2 cut(s) 101, 712
NspV TTCGAA 1 cut(s) 450
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 222
PfeI GAWTC 4 cut(s) 5, 15, 69, 90
PflMI CCANNNNNTGG 1 cut(s) 646
PinAI ACCGGT 1 cut(s) 270
PleI GAGTC 2 cut(s) 393, 724
PpsI GAGTC 2 cut(s) 393, 724
Ppu21I YACGTR 1 cut(s) 226
PsiI TTATAA 1 cut(s) 795
Psp124BI GAGCTC 2 cut(s) 311, 715
Psp6I CCWGG 1 cut(s) 706
PspGI CCWGG 1 cut(s) 706
PspN4I GGNNCC 3 cut(s) 134, 622, 638
PspPI GGNCC 1 cut(s) 165
PsuI RGATCY 3 cut(s) 132, 421, 636
RsaI GTAC 5 cut(s) 147, 294, 344, 492, 656
RsaNI GTAC 5 cut(s) 146, 293, 343, 491, 655
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 2 cut(s) 311, 715
SaqAI TTAA 5 cut(s) 150, 251, 369, 426, 777
Sau3AI GATC 8 cut(s) 103, 132, 181, 421, 433, 636, 690, 769
Sau96I GGNCC 1 cut(s) 165
ScaI AGTACT 2 cut(s) 147, 656
SchI GAGTC 2 cut(s) 393, 724
ScrFI CCNGG 3 cut(s) 282, 549, 708
SduI GDGCHC 2 cut(s) 311, 715
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 6 cut(s) 41, 54, 194, 600, 641, 799
SspMI CTAG 2 cut(s) 51, 396
SstI GAGCTC 2 cut(s) 311, 715
StyD4I CCNGG 3 cut(s) 280, 547, 706
TaaI ACNGT 5 cut(s) 191, 213, 292, 495, 632
TaiI ACGT 1 cut(s) 228
TaqI TCGA 8 cut(s) 13, 93, 109, 287, 402, 444, 450, 465
TasI AATT 6 cut(s) 41, 54, 194, 600, 641, 799
TatI WGTACW 4 cut(s) 145, 292, 490, 654
TfiI GAWTC 4 cut(s) 5, 15, 69, 90
Tru1I TTAA 5 cut(s) 150, 251, 369, 426, 777
Tru9I TTAA 5 cut(s) 150, 251, 369, 426, 777
TspDTI ATGAA 4 cut(s) 111, 192, 229, 444
Van91I CCANNNNNTGG 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 165
XapI RAATTY 3 cut(s) 54, 194, 799
XspI CTAG 2 cut(s) 51, 396
ZrmI AGTACT 2 cut(s) 147, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.