Rh2AG650200

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015187
Physical Location & Seq
Forward (+)
86904469 .. 86906022
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG650200.1

Sequence Viewer

Length: 879 bp
ATGGTCACCATTCATCCAAACCTCAAGCCATGGCATCAGTCCATGACAAGCAACCAGCAGTGCTACTCACACACCACCCTCTCTCCTCTATGCACCACCAAACCATCAACAATTTCATGCACTGCTTCATCAGAAAGCAATCAATCTTCCCCAGCAGGCAGAATCAAACGCCTTGTTCTTCCCCAAGAGGGCAGAACCAAACTCAACACTTACCCTGACAGAGATTTCTATGCCTATCCGCGCTTCGTGACACATGTCGACGATGGCTTCATTTCCACATTGACCAATCTGTATAGAGAAAAGTTGAGACCCGACTCAGAGGTTCTTGATCTCATGAGCTCATGGGTTAGTCACTTGCCGAAAGAGGTACAGTATAAGAAAGTGGTGGGACATGGGCTAAATGCACAAGAGCTTGCCAAGAACCCTAGGCTGGATTACTTTTTTGTCAAGGATCTGAATCAGGATCAGAAGCTTGAGTTGGAAAGCTCTAGTTTTGATGCTGTGTTGTGCACGGTTAGCGTTCAGTATCTTCAACAACCTGAGAAGGTGTTTGCGGAGGTGTTCCGGGTTTTAAGACCAGGAGGGGTGTTCATAGTGAGCTTTAGCAATCGATTGTTTTACGAGAAAGCAATTGGAGCATGGAGAGATGGGAGTGCATACAGCCGGATACAATTAGTAGTGCAATATTTCCAATCTGTGGAAGGGTTCACAAAAGCTGAAATCGTTAAGAAATTACCCGATGCCAGTGGTGGTGGTGCTGCAGAGAATAAATCACCATTCAGTTGGTTCATGGGTTTGCTTGGATTGCTGTCCGGGTCTGATCCCTTCTATGCAGTCTTAGCTTACAAGAACTTCAAACCTGTATACGAAACAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

292

Amino Acids

33.01

Weight (kDa)

8.52

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 135 - 197 6.3e-07 Methyltransferase domain
Methyltransf_25 PF13649 147 - 195 6.1e-07 Methyltransferase domain
Methyltransf_11 PF08241 148 - 199 5.8e-11 Methyltransferase domain
Methyltransf_31 PF13847 150 - 203 1.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01660
fragaria_vesca FvH4_6g52050
malus_domestica MD09G1020500.v1.1
prunus_persica Prupe.3G295700_v2.0.a1
pyrus_communis pycom111g01720
rosa_chinensis RchiOBHm_Chr2g0173561
rosa_laevigata RLG00000022202
rosa_multiflora Rmu_sc0002538.1_g000024 Rmu_sc0029872.1_g000002
rosa_roxburghii Rroxscaffold_2G00078780
rosa_rugosa Rorug02G0571400
rosa_samantha Rh2AG650200 Rh2BG660600 Rh2CG625500 Rh2DG675900
rosa_wichuraiana Rw2G053360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 697
AccI GTMKAC 2 cut(s) 258, 864
AccII CGCG 1 cut(s) 241
AciI CCGC 2 cut(s) 239, 554
AclWI GGATC 3 cut(s) 459, 471, 815
AfaI GTAC 1 cut(s) 369
AfiI CCNNNNNNNGG 3 cut(s) 188, 430, 697
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 2 cut(s) 533, 856
AjnI CCWGG 1 cut(s) 577
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 7 cut(s) 339, 412, 472, 486, 600, 716, 842
AluI AGCT 7 cut(s) 339, 412, 472, 486, 600, 716, 842
Alw21I GWGCWC 2 cut(s) 341, 512
Alw26I GTCTC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 508
AlwI GGATC 3 cut(s) 459, 471, 815
ApaLI GTGCAC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 758
AspA2I CCTAGG 1 cut(s) 425
AspLEI GCGC 1 cut(s) 243
AsuC2I CCSGG 2 cut(s) 566, 814
AsuHPI GGTGA 1 cut(s) 765
AvrII CCTAGG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 512
BanII GRGCYC 1 cut(s) 341
Bbv12I GWGCWC 2 cut(s) 341, 512
BbvI GCAGC 1 cut(s) 745
BccI CCATC 3 cut(s) 112, 257, 641
BciT130I CCWGG 1 cut(s) 579
BciVI GTATCC 1 cut(s) 660
BcnI CCSGG 2 cut(s) 566, 814
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 426, 489
BfmI CTRYAG 1 cut(s) 759
BfuI GTATCC 1 cut(s) 660
BisI GCNGC 1 cut(s) 759
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 1 cut(s) 760
Bme1390I CCNGG 3 cut(s) 566, 579, 814
BmrFI CCNGG 3 cut(s) 566, 579, 814
BmsI GCATC 3 cut(s) 43, 487, 730
BoxI GACNNNNGTC 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 8, 494
BpuMI CCSGG 2 cut(s) 566, 814
Bsa29I ATCGAT 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 29, 425
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 430, 697
Bse1I ACTGG 1 cut(s) 744
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 579
BseCI ATCGAT 1 cut(s) 610
BseDI CCNNGG 2 cut(s) 29, 425
BseGI GGATG 1 cut(s) 13
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 188, 430, 697
BseMII CTCAG 2 cut(s) 330, 531
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 1 cut(s) 745
BseYI CCCAGC 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 610
BsiHKAI GWGCWC 2 cut(s) 341, 512
BsiSI CCGG 3 cut(s) 565, 664, 813
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 3 cut(s) 188, 430, 697
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 402
Bso31I GGTCTC 1 cut(s) 301
Bsp1286I GDGCHC 2 cut(s) 341, 512
Bsp143I GATC 4 cut(s) 328, 451, 463, 820
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 2 cut(s) 239, 554
BspCNI CTCAG 2 cut(s) 329, 532
BspDI ATCGAT 1 cut(s) 610
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 333
BspMAI CTGCAG 1 cut(s) 763
BspPI GGATC 3 cut(s) 459, 471, 815
BspTNI GGTCTC 1 cut(s) 301
BsrI ACTGG 1 cut(s) 744
BssECI CCNNGG 2 cut(s) 29, 425
BssMI GATC 4 cut(s) 328, 451, 463, 820
BssNAI GTATAC 1 cut(s) 865
BssT1I CCWWGG 2 cut(s) 29, 425
Bst1107I GTATAC 1 cut(s) 865
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 372, 514
BstC8I GCNNGC 2 cut(s) 157, 414
BstDEI CTNAG 3 cut(s) 316, 540, 838
BstDSI CCRYGG 1 cut(s) 29
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 13
BstFNI CGCG 1 cut(s) 241
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 4 cut(s) 331, 454, 466, 823
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 4 cut(s) 328, 451, 463, 820
BstMWI GCNNNNNNNGC 4 cut(s) 516, 635, 805, 839
BstNI CCWGG 1 cut(s) 579
BstNSI RCATGY 1 cut(s) 257
BstPAI GACNNNNGTC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 3 cut(s) 564, 577, 812
BstSFI CTRYAG 1 cut(s) 759
BstSLI GKGCMC 1 cut(s) 512
BstUI CGCG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 745
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 783
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 865
Bsu15I ATCGAT 1 cut(s) 610
BsuI GTATCC 1 cut(s) 660
BsuTUI ATCGAT 1 cut(s) 610
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 1 cut(s) 13
BtsI GCAGTG 2 cut(s) 65, 120
BtsIMutI CAGTG 3 cut(s) 65, 120, 751
Cac8I GCNNGC 2 cut(s) 157, 414
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 610
Csp6I GTAC 1 cut(s) 368
CviAII CATG 9 cut(s) 30, 43, 117, 254, 334, 342, 392, 639, 790
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 3 cut(s) 316, 540, 838
DpnI GATC 4 cut(s) 330, 453, 465, 822
DpnII GATC 4 cut(s) 328, 451, 463, 820
Ecl136II GAGCTC 1 cut(s) 339
Eco130I CCWWGG 2 cut(s) 29, 425
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 301
Eco53kI GAGCTC 1 cut(s) 339
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 339
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 577
EcoT14I CCWWGG 2 cut(s) 29, 425
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 2 cut(s) 29, 425
FaeI CATG 9 cut(s) 33, 46, 120, 257, 337, 345, 395, 642, 793
FaqI GGGAC 1 cut(s) 402
FatI CATG 9 cut(s) 29, 42, 116, 253, 333, 341, 391, 638, 789
FblI GTMKAC 2 cut(s) 258, 864
Fnu4HI GCNGC 1 cut(s) 759
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 759
FspBI CTAG 2 cut(s) 426, 489
GlaI GCGC 1 cut(s) 242
GluI GCNGC 1 cut(s) 759
GsaI CCCAGC 1 cut(s) 155
HapII CCGG 3 cut(s) 565, 664, 813
HhaI GCGC 1 cut(s) 243
Hin1II CATG 9 cut(s) 33, 46, 120, 257, 337, 345, 395, 642, 793
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 470
HinfI GANTC 3 cut(s) 162, 314, 457
HpaII CCGG 3 cut(s) 565, 664, 813
HphI GGTGA 1 cut(s) 765
Hpy166II GTNNAC 4 cut(s) 259, 510, 708, 865
Hpy188I TCNGA 5 cut(s) 133, 319, 456, 468, 820
Hpy188III TCNNGA 4 cut(s) 247, 326, 334, 461
Hpy8I GTNNAC 4 cut(s) 259, 510, 708, 865
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 3 cut(s) 538, 695, 835
HpyCH4III ACNGT 2 cut(s) 372, 514
HpyCH4V TGCA 8 cut(s) 93, 120, 404, 510, 656, 682, 761, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 516, 635, 805, 839
HpyF3I CTNAG 3 cut(s) 316, 540, 838
Hsp92II CATG 9 cut(s) 33, 46, 120, 257, 337, 345, 395, 642, 793
HspAI GCGC 1 cut(s) 241
Kzo9I GATC 4 cut(s) 328, 451, 463, 820
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 1 cut(s) 745
LweI GCATC 3 cut(s) 43, 487, 730
MaeI CTAG 2 cut(s) 426, 489
MaeIII GTNAC 3 cut(s) 4, 247, 350
MalI GATC 4 cut(s) 330, 453, 465, 822
MboI GATC 4 cut(s) 328, 451, 463, 820
MboII GAAGA 3 cut(s) 138, 170, 521
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 2 cut(s) 341, 512
MluCI AATT 4 cut(s) 111, 630, 671, 731
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 8 cut(s) 32, 89, 96, 181, 313, 358, 550, 575
MseI TTAA 2 cut(s) 572, 726
MspI CCGG 3 cut(s) 565, 664, 813
MspR9I CCNGG 3 cut(s) 566, 579, 814
MunI CAATTG 1 cut(s) 630
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 241
MwoI GCNNNNNNNGC 4 cut(s) 516, 635, 805, 839
NciI CCSGG 2 cut(s) 566, 814
NcoI CCATGG 1 cut(s) 29
NdeII GATC 4 cut(s) 328, 451, 463, 820
NlaIII CATG 9 cut(s) 33, 46, 120, 257, 337, 345, 395, 642, 793
NmuCI GTSAC 3 cut(s) 4, 247, 350
NspI RCATGY 1 cut(s) 257
PagI TCATGA 1 cut(s) 333
PciI ACATGT 1 cut(s) 253
PfeI GAWTC 2 cut(s) 162, 457
PflMI CCANNNNNTGG 1 cut(s) 697
PkrI GCNGC 1 cut(s) 760
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PscI ACATGT 1 cut(s) 253
PshAI GACNNNNGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 577
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 1 cut(s) 577
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
SacI GAGCTC 1 cut(s) 341
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 2 cut(s) 572, 726
SatI GCNGC 1 cut(s) 759
Sau3AI GATC 4 cut(s) 328, 451, 463, 820
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 566, 579, 814
SduI GDGCHC 2 cut(s) 341, 512
SfaNI GCATC 3 cut(s) 43, 487, 730
SfcI CTRYAG 1 cut(s) 759
SmlI CTYRAG 2 cut(s) 23, 473
SmoI CTYRAG 2 cut(s) 23, 473
Sse9I AATT 4 cut(s) 111, 630, 671, 731
SsiI CCGC 2 cut(s) 239, 554
SspI AATATT 1 cut(s) 686
SspMI CTAG 2 cut(s) 426, 489
SstI GAGCTC 1 cut(s) 341
StyD4I CCNGG 3 cut(s) 564, 577, 812
StyI CCWWGG 2 cut(s) 29, 425
TaaI ACNGT 2 cut(s) 372, 514
TaqI TCGA 2 cut(s) 258, 610
TasI AATT 4 cut(s) 111, 630, 671, 731
TfiI GAWTC 2 cut(s) 162, 457
Tru1I TTAA 2 cut(s) 572, 726
Tru9I TTAA 2 cut(s) 572, 726
TscAI CASTG 3 cut(s) 65, 127, 751
TseFI GTSAC 3 cut(s) 4, 247, 350
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 3 cut(s) 4, 247, 350
TspDTI ATGAA 5 cut(s) 105, 117, 259, 580, 778
TspRI CASTG 3 cut(s) 65, 127, 751
Van91I CCANNNNNTGG 1 cut(s) 697
VneI GTGCAC 1 cut(s) 508
XceI RCATGY 1 cut(s) 257
XmaJI CCTAGG 1 cut(s) 425
XmiI GTMKAC 2 cut(s) 258, 864
XspI CTAG 2 cut(s) 426, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.