Rorug02G0571400

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015187
Physical Location & Seq
Forward (+)
69758124 .. 69759503
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0571400.1

Sequence Viewer

Length: 1257 bp
ATGGCACTCCCAAACTCATCAGTAAAAGTAGTTCAAGTTTGCAGGGTGGCTCCGCCACTTCCAGCCTCACCGGATCACTCCCTTCCCCTGACCTTCTTCGACATACGTTGGTTAAGGTTTGCACCACCGCAGCTCCTCTATTTCTACAAATTATCTTCTTCCTCTAACAATTCGACCTTCTCATTTGATTCCATACTCGACAAACTCAAAACCTCACTCTCTCTTACCCTCAAACAGTTCCTGCCTCTCGCGGGAAACCTCACTTGGCCTCAAGACTCCCTCAAACCCATTCTGAGCTATGTCCAAGGAGACACCCTTTTGCTTACAATAGTCGAATCCAATGCTGATTTTGACCGCCTTTCGAGCAATTACAACCTGCTTGAAGCCCAAGAATACCATCCTCTTGTACCCCAATTGGAGGTGTCTCATGAACGAGCTGCAGTGATGGCATTGCAAATCACTCTGTTTCCAAACAAAGGCTTTTCCATTGGAGCAGCAATGCACAAAGCAGTCCTTGATGGCTTAACTGCATTTTCATTTTTAAAATCATGGGCGCACATCTGCAAACATCAAGGGTTGACTTCATCTTCATTACCAGACCAGCTCAAACCCTTCTACGACAGAAGCCTCATCCAAGACCAGAAGGGGCTCGGAGCACTCTTCGCAAACCAATACCAAGCCATGGACGGTTCTGATACGATTCTCCGCTTGTCAACGTTTTCTCTAGCATGTGCCTATACATGGGTTTGCTTAGTAAAGGCTGAAGAAACAAAAGAAGATAAAGCACGAATCATCTTTTCCGTGGACTGTAGGTCTCGGCTAGACCCTCCTCTACCTGCAACATATTTCGGGAACTGCTTAGTGGGCTGTTTGGTAGTTGCAGAAACAAAAGATCTACTGGGAGAAGATGGGTTGATTGTGGCATTAAGTGCAATTAGTGAAGCTTTGAGGAACTTTGAAAAGAAGGGGATTTTGAACGGAGCTGAACATTGGGTTTCAATATTGAGCAGTGTGCGTACAGGTAGACTGGTTGGCATTGCTGGCTCACATCAGTTTGGTGCGTATGAAACTGATTTTGGATGGGGATCACCAAGCAAGGTTGAGGTTGTTTCCATAGATAGGACAGGAGCCATCTCCTTGACAGATAGCAAGAACGGTGGTGGAGGCGTTGACGTTGGGTTGGTCTTGAAGAAACATCACATGGATGCTTTCGCTTCTCTATTTGCCAAAGGTCTCAAAAACCATTCAAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

418

Amino Acids

45.73

Weight (kDa)

7.16

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 15 - 196 6e-20 Transferase family
Transferase PF02458 236 - 406 3e-26 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01660
fragaria_vesca FvH4_6g52050
malus_domestica MD09G1020500.v1.1
prunus_persica Prupe.3G295700_v2.0.a1
pyrus_communis pycom111g01720
rosa_chinensis RchiOBHm_Chr2g0173561
rosa_laevigata RLG00000022202
rosa_multiflora Rmu_sc0002538.1_g000024 Rmu_sc0029872.1_g000002
rosa_roxburghii Rroxscaffold_2G00078780
rosa_rugosa Rorug02G0571400
rosa_samantha Rh2AG650200 Rh2BG660600 Rh2CG625500 Rh2DG675900
rosa_wichuraiana Rw2G053360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 384, 844
AccB7I CCANNNNNTGG 1 cut(s) 682
AccI GTMKAC 1 cut(s) 1024
AccII CGCG 1 cut(s) 251
AciI CCGC 5 cut(s) 53, 128, 251, 355, 706
AclI AACGTT 1 cut(s) 716
AclWI GGATC 2 cut(s) 81, 1093
AcsI RAATTY 1 cut(s) 1251
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 2 cut(s) 408, 1018
AfiI CCNNNNNNNGG 6 cut(s) 251, 418, 476, 682, 741, 1119
AgsI TTSAA 7 cut(s) 35, 383, 959, 976, 999, 1189, 1248
AhdI GACNNNNNGTC 1 cut(s) 811
AjuI GAANNNNNNNTTGG 4 cut(s) 247, 279, 1059, 1091
AluBI AGCT 6 cut(s) 133, 297, 437, 604, 944, 983
AluI AGCT 6 cut(s) 133, 297, 437, 604, 944, 983
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 4 cut(s) 303, 429, 819, 1238
AlwI GGATC 2 cut(s) 81, 1093
AlwNI CAGNNNCTG 1 cut(s) 241
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 3 cut(s) 130, 437, 494
ApoI RAATTY 1 cut(s) 1251
AspLEI GCGC 1 cut(s) 556
AsuHPI GGTGA 2 cut(s) 60, 1080
BanII GRGCYC 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 3 cut(s) 142, 424, 506
BccI CCATC 6 cut(s) 405, 439, 512, 902, 1074, 1139
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BcoDI GTCTC 4 cut(s) 303, 429, 819, 1238
BfaI CTAG 2 cut(s) 725, 821
BfmI CTRYAG 2 cut(s) 438, 808
BfuAI ACCTGC 2 cut(s) 384, 844
BglII AGATCT 1 cut(s) 892
BisI GCNGC 3 cut(s) 131, 438, 495
BlsI GCNGC 3 cut(s) 132, 439, 496
BmeRI GACNNNNNGTC 1 cut(s) 811
BmiI GGNNCC 2 cut(s) 51, 1129
BmrI ACTGGG 1 cut(s) 908
BmsI GCATC 1 cut(s) 1195
BmuI ACTGGG 1 cut(s) 908
BpuEI CTTGAG 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 1084
BsaI GGTCTC 2 cut(s) 819, 1238
BsaJI CCNNGG 3 cut(s) 304, 681, 801
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 6 cut(s) 251, 418, 476, 682, 741, 1119
Bse1I ACTGG 2 cut(s) 903, 1032
Bse3DI GCAATG 3 cut(s) 449, 504, 1035
Bse8I GATNNNNATC 1 cut(s) 1084
BseDI CCNNGG 3 cut(s) 304, 681, 801
BseGI GGATG 4 cut(s) 397, 630, 1085, 1210
BseJI GATNNNNATC 1 cut(s) 1084
BseLI CCNNNNNNNGG 6 cut(s) 251, 418, 476, 682, 741, 1119
BseMI GCAATG 3 cut(s) 449, 504, 1035
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 903, 1032
BseRI GAGGAG 2 cut(s) 125, 819
BseXI GCAGC 3 cut(s) 142, 424, 506
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 658
BsiSI CCGG 1 cut(s) 71
BslI CCNNNNNNNGG 6 cut(s) 251, 418, 476, 682, 741, 1119
BsmAI GTCTC 4 cut(s) 303, 429, 819, 1238
BsnI GGCC 1 cut(s) 268
Bso31I GGTCTC 2 cut(s) 819, 1238
Bsp1286I GDGCHC 2 cut(s) 651, 658
Bsp143I GATC 3 cut(s) 73, 892, 1085
Bsp19I CCATGG 1 cut(s) 681
BspACI CCGC 5 cut(s) 53, 128, 251, 355, 706
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 251
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 51, 1129
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 2 cut(s) 384, 844
BspPI GGATC 2 cut(s) 81, 1093
BspTNI GGTCTC 2 cut(s) 819, 1238
BsrDI GCAATG 3 cut(s) 449, 504, 1035
BsrI ACTGG 2 cut(s) 903, 1032
BssECI CCNNGG 3 cut(s) 304, 681, 801
BssMI GATC 3 cut(s) 73, 892, 1085
BssT1I CCWWGG 2 cut(s) 304, 681
Bst4CI ACNGT 4 cut(s) 237, 689, 809, 1157
Bst6I CTCTTC 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 929
BstC8I GCNNGC 1 cut(s) 1042
BstDEI CTNAG 3 cut(s) 293, 751, 859
BstDSI CCRYGG 2 cut(s) 681, 801
BstF5I GGATG 4 cut(s) 397, 630, 1085, 1210
BstFNI CGCG 1 cut(s) 251
BstHHI GCGC 1 cut(s) 556
BstKTI GATC 3 cut(s) 76, 895, 1088
BstMAI GTCTC 4 cut(s) 303, 429, 819, 1238
BstMBI GATC 3 cut(s) 73, 892, 1085
BstMWI GCNNNNNNNGC 6 cut(s) 363, 446, 662, 864, 929, 1041
BstNSI RCATGY 1 cut(s) 732
BstSFI CTRYAG 2 cut(s) 438, 808
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 142, 424, 506
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
BsuRI GGCC 1 cut(s) 268
BtgI CCRYGG 2 cut(s) 681, 801
BtsCI GGATG 4 cut(s) 397, 630, 1085, 1210
BtsI GCAGTG 2 cut(s) 447, 1015
BtsIMutI CAGTG 2 cut(s) 447, 1015
BveI ACCTGC 2 cut(s) 384, 844
Cac8I GCNNGC 1 cut(s) 1042
CaiI CAGNNNCTG 1 cut(s) 241
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 556
Csp6I GTAC 2 cut(s) 407, 1017
CspCI CAANNNNNGTGG 2 cut(s) 1138, 1173
CviAII CATG 6 cut(s) 428, 549, 682, 729, 741, 1201
CviQI GTAC 2 cut(s) 407, 1017
DdeI CTNAG 3 cut(s) 293, 751, 859
DpnI GATC 3 cut(s) 75, 894, 1087
DpnII GATC 3 cut(s) 73, 892, 1085
DraI TTTAAA 1 cut(s) 543
DriI GACNNNNNGTC 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 665
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 665
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 304, 681
Eco24I GRGCYC 1 cut(s) 651
Eco31I GGTCTC 2 cut(s) 819, 1238
Eco57I CTGAAG 1 cut(s) 783
EcoT14I CCWWGG 2 cut(s) 304, 681
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 2 cut(s) 304, 681
FaeI CATG 6 cut(s) 431, 552, 685, 732, 744, 1204
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FatI CATG 6 cut(s) 427, 548, 681, 728, 740, 1200
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 1 cut(s) 1024
Fnu4HI GCNGC 3 cut(s) 131, 438, 495
FokI GGATG 4 cut(s) 384, 617, 1092, 1217
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 3 cut(s) 131, 438, 495
FspBI CTAG 2 cut(s) 725, 821
GlaI GCGC 1 cut(s) 555
GluI GCNGC 3 cut(s) 131, 438, 495
HaeIII GGCC 1 cut(s) 268
HapII CCGG 1 cut(s) 71
HhaI GCGC 1 cut(s) 556
Hin1II CATG 6 cut(s) 431, 552, 685, 732, 744, 1204
Hin6I GCGC 1 cut(s) 554
HinP1I GCGC 1 cut(s) 554
HincII GTYRAC 3 cut(s) 579, 714, 1171
HindII GTYRAC 3 cut(s) 579, 714, 1171
HindIII AAGCTT 1 cut(s) 942
HinfI GANTC 5 cut(s) 188, 275, 335, 700, 789
HpaII CCGG 1 cut(s) 71
HphI GGTGA 2 cut(s) 60, 1080
Hpy166II GTNNAC 5 cut(s) 579, 714, 805, 1025, 1171
Hpy188I TCNGA 3 cut(s) 294, 653, 694
Hpy188III TCNNGA 5 cut(s) 272, 428, 850, 1186, 1248
Hpy8I GTNNAC 5 cut(s) 579, 714, 805, 1025, 1171
HpyAV CCTTC 6 cut(s) 92, 103, 187, 622, 637, 958
HpyCH4III ACNGT 4 cut(s) 237, 689, 809, 1157
HpyCH4IV ACGT 3 cut(s) 106, 716, 1173
HpyF10VI GCNNNNNNNGC 6 cut(s) 363, 446, 662, 864, 929, 1041
HpyF3I CTNAG 3 cut(s) 293, 751, 859
HpySE526I ACGT 3 cut(s) 106, 716, 1173
Hsp92II CATG 6 cut(s) 431, 552, 685, 732, 744, 1204
HspAI GCGC 1 cut(s) 554
Kzo9I GATC 3 cut(s) 73, 892, 1085
LmnI GCTCC 6 cut(s) 55, 138, 491, 653, 980, 1127
Lsp1109I GCAGC 3 cut(s) 142, 424, 506
LweI GCATC 1 cut(s) 1195
MaeI CTAG 2 cut(s) 725, 821
MaeII ACGT 3 cut(s) 106, 716, 1173
MalI GATC 3 cut(s) 75, 894, 1087
MboI GATC 3 cut(s) 73, 892, 1085
MboII GAAGA 9 cut(s) 88, 147, 150, 579, 652, 776, 788, 917, 1201
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 2 cut(s) 651, 658
MluCI AATT 6 cut(s) 149, 169, 367, 413, 933, 1251
MlyI GAGTC 1 cut(s) 269
MseI TTAA 4 cut(s) 113, 524, 542, 926
MslI CAYNNNNRTG 1 cut(s) 1203
MspI CCGG 1 cut(s) 71
MunI CAATTG 1 cut(s) 413
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 6 cut(s) 363, 446, 662, 864, 929, 1041
NcoI CCATGG 1 cut(s) 681
NdeII GATC 3 cut(s) 73, 892, 1085
NlaIII CATG 6 cut(s) 431, 552, 685, 732, 744, 1204
NlaIV GGNNCC 2 cut(s) 51, 1129
NmeAIII GCCGAG 1 cut(s) 796
NspI RCATGY 1 cut(s) 732
PagI TCATGA 1 cut(s) 427
PfeI GAWTC 4 cut(s) 188, 335, 700, 789
PflMI CCANNNNNTGG 1 cut(s) 682
PkrI GCNGC 3 cut(s) 132, 439, 496
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Psp1406I AACGTT 1 cut(s) 716
PspN4I GGNNCC 2 cut(s) 51, 1129
PsrI GAACNNNNNNTAC 2 cut(s) 15, 47
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 1 cut(s) 892
RsaI GTAC 2 cut(s) 408, 1018
RsaNI GTAC 2 cut(s) 407, 1017
RseI CAYNNNNRTG 1 cut(s) 1203
SaqAI TTAA 4 cut(s) 113, 524, 542, 926
SatI GCNGC 3 cut(s) 131, 438, 495
Sau3AI GATC 3 cut(s) 73, 892, 1085
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 651, 658
SfaNI GCATC 1 cut(s) 1195
SfcI CTRYAG 2 cut(s) 438, 808
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 1 cut(s) 270
SmoI CTYRAG 1 cut(s) 270
Sse9I AATT 6 cut(s) 149, 169, 367, 413, 933, 1251
SsiI CCGC 5 cut(s) 53, 128, 251, 355, 706
SspI AATATT 1 cut(s) 1002
SspMI CTAG 2 cut(s) 725, 821
StyI CCWWGG 2 cut(s) 304, 681
TaaI ACNGT 4 cut(s) 237, 689, 809, 1157
TaiI ACGT 3 cut(s) 109, 719, 1176
TaqI TCGA 5 cut(s) 99, 173, 198, 333, 362
TasI AATT 6 cut(s) 149, 169, 367, 413, 933, 1251
TfiI GAWTC 4 cut(s) 188, 335, 700, 789
Tru1I TTAA 4 cut(s) 113, 524, 542, 926
Tru9I TTAA 4 cut(s) 113, 524, 542, 926
TscAI CASTG 2 cut(s) 447, 1015
TseI GCWGC 3 cut(s) 130, 437, 494
TspDTI ATGAA 5 cut(s) 444, 525, 573, 579, 1080
TspGWI ACGGA 2 cut(s) 790, 993
TspRI CASTG 2 cut(s) 447, 1015
Van91I CCANNNNNTGG 1 cut(s) 682
XapI RAATTY 1 cut(s) 1251
XceI RCATGY 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 1024
XspI CTAG 2 cut(s) 725, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.