pycom111g01720

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0015187
Physical Location & Seq
Reverse (-)
1058364 .. 1059889
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01720.2

Sequence Viewer

Length: 864 bp
ATGGTCACAATTCATCCAAACCTCAAACCATGGCATCCATTTCTTACAAGCAACCATAAGACCAAACCTTTCCTACTTCCATCAACAATCTCCTGCACTTCTTCTAGTGAAAACAATGGAACCAGTAAGCAATCTTCATCCTCAGCTGGCAACATCAAACGTCTTGTTCTTCCCCAAGAGGGCAGAAGAAAACTCAACACTTATCCGGACAGAGACTTCTACGCTTATCCGCGATTCGTGACGCATGTCGATGACGGCTTCATTGCCACACTGACCAATCTCTACAGGCAGAGGCTGAGGCCTGGCTCAGAGATTCTTGATCTTATGAGTTCGTGGATCAGCCATTTGCCGAAAGAAGTTGAGTACAAGAAAGTTGTGGGACATGGGCTCAATGCACAGGAGCTTGCCAGGAACCCTAGGTTGGATTACTTTTTTGTCAAGGATCTGAACCAGGATCAGAAGCTTGAGTTGGCGAGCAGCAGTTTTGATGCAGTGTTGTGTACAGTCAGTGTGCAGTATCTTCAACAGCCTGAGAAGGTGTTTGCAGAGGTTTTTAGGGTTCTGAGGCCAGGAGGGGTGTTTATTGTGAGCTTTAGCAATCGGTTGTTCTATGAGAAAGCCATTGGGGCATGGAGAGATGGGACTGCATACAGCAGGACGCAGTTGGTTGTGCAGTACTTCCAATCTGTGGAAGGTTTCACACAAGCTGAAGTAATTAAGAAATTACCAGATGCTAATGGTGGTGGTGGTTCACAAAGCAAATCACCACTCGGTTGGTTCATGGGCTTCCTGGGGTTGCCGTCTGGTTCAGGTCCATTTTATGCAGTGGCAGCTTACAAGAACTTCAAACCTGTATATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

288

Amino Acids

32.28

Weight (kDa)

9.42

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01660
fragaria_vesca FvH4_6g52050
malus_domestica MD09G1020500.v1.1
prunus_persica Prupe.3G295700_v2.0.a1
pyrus_communis pycom111g01720
rosa_chinensis RchiOBHm_Chr2g0173561
rosa_laevigata RLG00000022202
rosa_multiflora Rmu_sc0002538.1_g000024 Rmu_sc0029872.1_g000002
rosa_roxburghii Rroxscaffold_2G00078780
rosa_rugosa Rorug02G0571400
rosa_samantha Rh2AG650200 Rh2BG660600 Rh2CG625500 Rh2DG675900
rosa_wichuraiana Rw2G053360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 688
AccII CGCG 1 cut(s) 232
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 1 cut(s) 230
AclWI GGATC 3 cut(s) 344, 450, 462
AcuI CTGAAG 1 cut(s) 729
AfaI GTAC 3 cut(s) 365, 502, 677
AfiI CCNNNNNNNGG 3 cut(s) 179, 421, 688
AgsI TTSAA 2 cut(s) 524, 847
AjnI CCWGG 5 cut(s) 301, 407, 450, 568, 789
AjuI GAANNNNNNNTTGG 4 cut(s) 404, 436, 452, 484
AluBI AGCT 6 cut(s) 146, 403, 463, 591, 707, 833
AluI AGCT 6 cut(s) 146, 403, 463, 591, 707, 833
Alw26I GTCTC 1 cut(s) 207
AlwI GGATC 3 cut(s) 344, 450, 462
AlwNI CAGNNNCTG 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 299, 566
ApeKI GCWGC 2 cut(s) 477, 830
AspA2I CCTAGG 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 812
AsuHPI GGTGA 1 cut(s) 756
AvaII GGWCC 1 cut(s) 812
AvrII CCTAGG 1 cut(s) 416
BanII GRGCYC 1 cut(s) 390
BarI GAAGNNNNNNTAC 2 cut(s) 118, 150
BbvCI CCTCAGC 2 cut(s) 142, 296
BbvI GCAGC 2 cut(s) 489, 842
BccI CCATC 2 cut(s) 88, 632
BceAI ACGGC 2 cut(s) 271, 784
BciT130I CCWGG 5 cut(s) 303, 409, 452, 570, 791
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 2 cut(s) 105, 417
BfmI CTRYAG 1 cut(s) 283
BglI GCCNNNNNGGC 1 cut(s) 626
BisI GCNGC 2 cut(s) 478, 831
BlnI CCTAGG 1 cut(s) 416
BlsI GCNGC 2 cut(s) 479, 832
BmcAI AGTACT 1 cut(s) 677
Bme1390I CCNGG 5 cut(s) 303, 409, 452, 570, 791
Bme18I GGWCC 1 cut(s) 812
BmgT120I GGNCC 1 cut(s) 812
BmiI GGNNCC 2 cut(s) 121, 413
BmrFI CCNGG 5 cut(s) 303, 409, 452, 570, 791
BmsI GCATC 3 cut(s) 43, 478, 721
BoxI GACNNNNGTC 1 cut(s) 245
Bpu10I CCTNAGC 2 cut(s) 142, 296
BpuEI CTTGAG 1 cut(s) 485
BsaJI CCNNGG 3 cut(s) 29, 416, 790
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 421, 688
Bse1I ACTGG 1 cut(s) 123
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 5 cut(s) 303, 409, 452, 570, 791
BseDI CCNNGG 3 cut(s) 29, 416, 790
BseGI GGATG 3 cut(s) 13, 34, 137
BseLI CCNNNNNNNGG 3 cut(s) 179, 421, 688
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 5 cut(s) 156, 287, 321, 522, 554
BseNI ACTGG 1 cut(s) 123
BseXI GCAGC 2 cut(s) 489, 842
BsgI GTGCAG 3 cut(s) 79, 533, 692
Bsh1236I CGCG 1 cut(s) 232
BshFI GGCC 2 cut(s) 301, 568
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 2 cut(s) 393, 655
BslI CCNNNNNNNGG 3 cut(s) 179, 421, 688
BsmAI GTCTC 1 cut(s) 207
BsmFI GGGAC 2 cut(s) 393, 655
BsnI GGCC 2 cut(s) 301, 568
Bsp1286I GDGCHC 1 cut(s) 390
Bsp13I TCCGGA 1 cut(s) 205
Bsp1407I TGTACA 1 cut(s) 500
Bsp143I GATC 4 cut(s) 319, 336, 442, 454
Bsp19I CCATGG 1 cut(s) 29
BspACI CCGC 1 cut(s) 230
BspANI GGCC 2 cut(s) 301, 568
BspCNI CTCAG 5 cut(s) 155, 288, 320, 523, 555
BspEI TCCGGA 1 cut(s) 205
BspFNI CGCG 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 121, 413
BspPI GGATC 3 cut(s) 344, 450, 462
BsrDI GCAATG 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 500
BsrI ACTGG 1 cut(s) 123
BssECI CCNNGG 3 cut(s) 29, 416, 790
BssMI GATC 4 cut(s) 319, 336, 442, 454
BssT1I CCWWGG 2 cut(s) 29, 416
Bst2UI CCWGG 5 cut(s) 303, 409, 452, 570, 791
Bst4CI ACNGT 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 500
BstC8I GCNNGC 3 cut(s) 148, 405, 475
BstDEI CTNAG 5 cut(s) 142, 296, 307, 531, 563
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 3 cut(s) 13, 34, 137
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 4 cut(s) 322, 339, 445, 457
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 4 cut(s) 319, 336, 442, 454
BstMWI GCNNNNNNNGC 2 cut(s) 626, 830
BstNI CCWGG 5 cut(s) 303, 409, 452, 570, 791
BstNSI RCATGY 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 245
BstSCI CCNGG 5 cut(s) 301, 407, 450, 568, 789
BstSFI CTRYAG 1 cut(s) 283
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 2 cut(s) 489, 842
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 774
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 2 cut(s) 301, 568
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 3 cut(s) 13, 34, 137
BtsI GCAGTG 2 cut(s) 498, 831
BtsIMutI CAGTG 4 cut(s) 269, 498, 514, 831
Cac8I GCNNGC 3 cut(s) 148, 405, 475
CaiI CAGNNNCTG 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 812
CseI GACGC 2 cut(s) 250, 667
Csp6I GTAC 3 cut(s) 364, 501, 676
CviAII CATG 5 cut(s) 30, 245, 383, 630, 781
CviQI GTAC 3 cut(s) 364, 501, 676
DdeI CTNAG 5 cut(s) 142, 296, 307, 531, 563
DpnI GATC 4 cut(s) 321, 338, 444, 456
DpnII GATC 4 cut(s) 319, 336, 442, 454
Eco130I CCWWGG 2 cut(s) 29, 416
Eco147I AGGCCT 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 812
Eco57I CTGAAG 1 cut(s) 729
EcoRII CCWGG 5 cut(s) 301, 407, 450, 568, 789
EcoT14I CCWWGG 2 cut(s) 29, 416
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 2 cut(s) 29, 416
FaeI CATG 5 cut(s) 33, 248, 386, 633, 784
FaqI GGGAC 2 cut(s) 393, 655
FatI CATG 5 cut(s) 29, 244, 382, 629, 780
Fnu4HI GCNGC 2 cut(s) 478, 831
FokI GGATG 2 cut(s) 21, 124
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 2 cut(s) 478, 831
FspBI CTAG 2 cut(s) 105, 417
GluI GCNGC 2 cut(s) 478, 831
HaeIII GGCC 2 cut(s) 301, 568
HapII CCGG 1 cut(s) 206
HgaI GACGC 2 cut(s) 250, 667
Hin1II CATG 5 cut(s) 33, 248, 386, 633, 784
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 2 cut(s) 234, 313
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 756
Hpy166II GTNNAC 2 cut(s) 501, 752
Hpy188I TCNGA 4 cut(s) 310, 447, 459, 564
Hpy188III TCNNGA 3 cut(s) 206, 238, 317
Hpy8I GTNNAC 2 cut(s) 501, 752
HpyAV CCTTC 2 cut(s) 529, 686
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 8 cut(s) 96, 395, 491, 514, 545, 647, 673, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 626, 830
HpyF3I CTNAG 5 cut(s) 142, 296, 307, 531, 563
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 5 cut(s) 33, 248, 386, 633, 784
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 4 cut(s) 319, 336, 442, 454
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 2 cut(s) 489, 842
LweI GCATC 3 cut(s) 43, 478, 721
MaeI CTAG 2 cut(s) 105, 417
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 4, 238
MalI GATC 4 cut(s) 321, 338, 444, 456
MboI GATC 4 cut(s) 319, 336, 442, 454
MboII GAAGA 5 cut(s) 93, 126, 161, 198, 512
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 3 cut(s) 9, 714, 722
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 8 cut(s) 32, 151, 172, 285, 291, 541, 558, 566
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 717
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 5 cut(s) 303, 409, 452, 570, 791
MvaI CCWGG 5 cut(s) 303, 409, 452, 570, 791
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 2 cut(s) 626, 830
NcoI CCATGG 1 cut(s) 29
NdeII GATC 4 cut(s) 319, 336, 442, 454
NlaIII CATG 5 cut(s) 33, 248, 386, 633, 784
NlaIV GGNNCC 2 cut(s) 121, 413
NmuCI GTSAC 2 cut(s) 4, 238
NspI RCATGY 1 cut(s) 248
PceI AGGCCT 1 cut(s) 301
PfeI GAWTC 2 cut(s) 234, 313
PflMI CCANNNNNTGG 1 cut(s) 688
PkrI GCNGC 2 cut(s) 479, 832
PshAI GACNNNNGTC 1 cut(s) 245
Psp6I CCWGG 5 cut(s) 301, 407, 450, 568, 789
PspGI CCWGG 5 cut(s) 301, 407, 450, 568, 789
PspN4I GGNNCC 2 cut(s) 121, 413
PspPI GGNCC 1 cut(s) 812
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 442
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 365, 502, 677
RsaNI GTAC 3 cut(s) 364, 501, 676
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 2 cut(s) 478, 831
Sau3AI GATC 4 cut(s) 319, 336, 442, 454
Sau96I GGNCC 1 cut(s) 812
ScaI AGTACT 1 cut(s) 677
ScrFI CCNGG 5 cut(s) 303, 409, 452, 570, 791
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 3 cut(s) 43, 478, 721
SfcI CTRYAG 1 cut(s) 283
SinI GGWCC 1 cut(s) 812
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 3 cut(s) 9, 714, 722
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 1 cut(s) 230
SspMI CTAG 2 cut(s) 105, 417
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 5 cut(s) 301, 407, 450, 568, 789
StyI CCWWGG 2 cut(s) 29, 416
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 249
TasI AATT 3 cut(s) 9, 714, 722
TatI WGTACW 3 cut(s) 363, 500, 675
TfiI GAWTC 2 cut(s) 234, 313
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 4 cut(s) 276, 498, 514, 831
TseFI GTSAC 2 cut(s) 4, 238
TseI GCWGC 2 cut(s) 477, 830
Tsp45I GTSAC 2 cut(s) 4, 238
TspDTI ATGAA 3 cut(s) 126, 250, 769
TspRI CASTG 4 cut(s) 276, 498, 514, 831
Van91I CCANNNNNTGG 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 812
XceI RCATGY 1 cut(s) 248
XmaJI CCTAGG 1 cut(s) 416
XspI CTAG 2 cut(s) 105, 417
ZrmI AGTACT 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.