AT3G05350

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011079
Physical Location & Seq
Reverse (-)
1526963 .. 1533909
6947 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05350.1

Sequence Viewer

Length: 2133 bp
ATGATTCCTCTAACTCTTTCGTCGCCGTCTCTGAATCGGCTCGTTTTATCCACATCACGTTACTCACACTCTCTATTTCTCAGCAATTTCAATTCGCTCTCTCTGATCCACCGGAAATTACCGTATAAACCTCTTTTTGGCGCAAGGTGTCATGCTAGTAGCAGCAGCAGCAGCTCTTCCTCCTTCACGGCAAAGTCTTCCAAAGAGATTCGTAAAGCTCAAACCAAAGTAGTTGTCGACGAGAAGCTTAGCTCGATCCGGCGACTGTTTTCGGAGCCGGGTGTTGGAATCGACGCTTATATCATACCTTCACAAGATGCACATCAGAGCGAGTTCATCGCAGAGTGCTATGCCAGGAGGGCTTATATATCAGGATTTACTGGAAGTGCTGGTACCGCTGTTGTCACCAAGGATAAGGCAGCTCTATGGACAGATGGCCGCTATTTTCTTCAGGCGGAGAAGCAATTAAACTCTAGTTGGATTCTCATGCGGGCTGGTAATCCGGGAGTCCCCACGGCGAGTGAATGGATAGCTGATGTTTTGGCGCCTGGTGGAAGAGTTGGTATTGACCCTTTCCTTTTTTCCGCCGATGCTGCTGAGGAATTGAAGGAGGTTATAGCAAAGAAGAATCATGAGTTGGTTTACTTGTACAATGTAAATCTTGTGGATGAGATATGGAAGGACTCAAGGCCAAAGCCTCCAAGCAGACAAATAAGGATACATGACTTGAAGTATGCTGGTTTAGATGTGGCCTCGAAATTGTTGTCTTTGAGGAACCAAATTATGGATGCTGGCACATCTGCCATTGTAATATCCATGCTTGATGAGATTGCATGGGTGCTTAATCTGAGAGGGAGCGATGTTCCACATTCACCTGTAATGTACGCATATCTGATTGTAGAGGTTGACCAAGCCCAACTTTTTGTAGATAATTCTAAAGTAACGGTGGAGGTGAAAGATCATTTGAAAAACGCAGGGATCGAACTTAGACCTTACGATTCCATTCTTCAAGGAATAGATAGTTTGGCAGCACGAGGAGCTCAGCTCTTGATGGATCCATCAACCCTCAACGTAGCTATCATTAGTACCTACAAATCTGCCTGTGAAAGATATTCGAGGAATTTTGAAAGCGAAGCCAAAGTTAAGACAAAGTTTACTGATAGCTCCAGCGGATATACTGCCAATCCTTCTGGTATTTACATGCAATCTCCTATTTCGTGGGCAAAGGCCATTAAAAATGATGCTGAGCTAAAGGGAATGAAGAATTCTCACTTGAGAGATGCTGCTGCCCTAGCTCATTTCTGGGCTTGGCTGGAAGAAGAAGTGCATAAGAATGCGAATCTAACAGAGGTCGATGTGGCTGACAGGCTTCTTGAATTCCGTTCAATGCAAGATGGTTTTATGGATACAAGCTTTGATACAATAAGTGGTTCCGGTGCAAATGGTGCTATTATACATTATAAGCCCGAGCCTGAGAGCTGCAGTCGTGTAGACCCTCAAAAACTTTTTTTGCTGGATAGTGGCGCTCAATATGTTGATGGAACAACAGACATCACCAGAACAGTACATTTTAGTGAACCTTCTGCCCGTGAAAAGGAATGTTTCACTCGAGTCTTGCAGGGTCATATAGCTCTTGATCAAGCCGTGTTCCCAGAAGGTACTCCTGGTTTTGTATTGGATGGGTTTGCACGCTCTTCTCTATGGAAAATTGGACTTGATTACCGCCATGGGACAGGACACGGCGTAGGTGCTGCACTCAATGTGCATGAAGGTCCTCAAAGTATTAGCTTTCGGTATGGAAACATGACCCCTCTTCAAAATGGCATGATAGTAAGCAATGAACCTGGATATTATGAAGACCATGCATTTGGGATCCGAATTGAGAATCTCCTTCATGTGAGAGATGCTGAAACACCAAACCGCTTTGGTGGTGCTACATACCTTGGATTTGAGAAGCTTACATTCTTCCCCATTCAGACAAAAATGGTTGATGTTTCTTTGCTGTCTGATACTGAGGTTGACTGGCTTAATAGTTACCACGCGGAAGTCTGGGAAAAGGTTTCACCATTATTGGAAGGTTCTACTACTCAGCAGTGGCTCTGGAACAACACAAGACCCTTAGCCAAACCATGA

Protein Analysis

710

Amino Acids

78.71

Weight (kDa)

6.08

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 96 - 209 1.8e-14 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 230 - 416 7.9e-45 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 419 - 634 8.8e-49 Metallopeptidase family M24
Peptidase_M24_C PF16188 647 - 707 2.1e-25 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1461
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 392, 544
AccB7I CCANNNNNTGG 1 cut(s) 784
AccI GTMKAC 2 cut(s) 237, 1491
AccII CGCG 1 cut(s) 2042
AciI CCGC 9 cut(s) 396, 439, 455, 490, 585, 1170, 1723, 1921, 2042
AclWI GGATC 7 cut(s) 100, 250, 986, 1049, 1062, 1867, 1880
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 1120, 1264, 1376
AcuI CTGAAG 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 545
AfaI GTAC 6 cut(s) 394, 650, 884, 1087, 1566, 1660
AfiI CCNNNNNNNGG 4 cut(s) 137, 284, 784, 1594
AgsI TTSAA 9 cut(s) 91, 607, 730, 967, 1010, 1127, 1376, 1386, 1817
AjnI CCWGG 4 cut(s) 353, 547, 1663, 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 7 cut(s) 100, 250, 986, 1049, 1062, 1867, 1880
Ama87I CYCGRG 2 cut(s) 1466, 1608
AoxI GGCC 4 cut(s) 436, 689, 750, 1227
ApoI RAATTY 3 cut(s) 1120, 1264, 1376
ArsI GACNNNNNNTTYG 6 cut(s) 219, 251, 749, 781, 1850, 1882
Asp718I GGTACC 1 cut(s) 392
AspLEI GCGC 3 cut(s) 143, 547, 1526
AspS9I GGNCC 1 cut(s) 1772
AsuC2I CCSGG 2 cut(s) 279, 504
AsuHPI GGTGA 5 cut(s) 397, 864, 964, 1546, 2055
AvaI CYCGRG 2 cut(s) 1466, 1608
AvaII GGWCC 1 cut(s) 1772
BaeI ACNNNNGTAYC 4 cut(s) 384, 417, 2001, 2034
BamHI GGATCC 2 cut(s) 1054, 1872
BanI GGYRCC 2 cut(s) 392, 544
BanII GRGCYC 1 cut(s) 1042
BarI GAAGNNNNNNTAC 2 cut(s) 376, 408
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 2 cut(s) 189, 1863
Bbv12I GWGCWC 1 cut(s) 1042
BbvCI CCTCAGC 1 cut(s) 597
BccI CCATC 6 cut(s) 428, 1045, 1066, 1388, 1532, 1673
BceAI ACGGC 5 cut(s) 10, 204, 531, 1628, 1756
BciT130I CCWGG 4 cut(s) 355, 549, 1665, 1845
BciVI GTATCC 2 cut(s) 711, 1399
BclI TGATCA 1 cut(s) 1636
BcnI CCSGG 2 cut(s) 279, 504
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 3 cut(s) 156, 474, 1292
BfmI CTRYAG 1 cut(s) 1480
BfoI RGCGCY 2 cut(s) 548, 1527
BfuI GTATCC 2 cut(s) 711, 1399
BglI GCCNNNNNGGC 1 cut(s) 359
BlpI GCTNAGC 3 cut(s) 248, 1041, 1245
Bme1390I CCNGG 6 cut(s) 279, 355, 504, 549, 1665, 1845
Bme18I GGWCC 1 cut(s) 1772
BmeT110I CYCGRG 2 cut(s) 1466, 1608
BmgT120I GGNCC 1 cut(s) 1772
BmiI GGNNCC 7 cut(s) 276, 394, 546, 776, 1056, 1432, 1874
BmrFI CCNGG 6 cut(s) 279, 355, 504, 549, 1665, 1845
BmsI GCATC 6 cut(s) 307, 580, 778, 1231, 1270, 1894
BpiI GAAGAC 2 cut(s) 189, 1863
BplI GAGNNNNNCTC 2 cut(s) 1029, 1061
BpmI CTGGAG 1 cut(s) 1150
Bpu10I CCTNAGC 2 cut(s) 597, 2119
Bpu1102I GCTNAGC 3 cut(s) 248, 1041, 1245
BpuEI CTTGAG 2 cut(s) 670, 1294
BpuMI CCSGG 2 cut(s) 279, 504
BsaBI GATNNNNATC 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 545
BsaJI CCNNGG 4 cut(s) 408, 513, 1726, 1942
BsaWI WCCGGW 2 cut(s) 111, 1433
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 284, 784, 1594
Bse1I ACTGG 2 cut(s) 385, 2027
Bse3DI GCAATG 1 cut(s) 1843
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 4 cut(s) 355, 549, 1665, 1845
BseDI CCNNGG 4 cut(s) 408, 513, 1726, 1942
BseGI GGATG 3 cut(s) 673, 793, 1684
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 4 cut(s) 137, 284, 784, 1594
BseMI GCAATG 1 cut(s) 1843
BseMII CTCAG 8 cut(s) 94, 588, 839, 1055, 1236, 1464, 2004, 2102
BseNI ACTGG 2 cut(s) 385, 2027
BseRI GAGGAG 1 cut(s) 1050
BsgI GTGCAG 1 cut(s) 1737
Bsh1236I CGCG 1 cut(s) 2042
BshFI GGCC 4 cut(s) 438, 691, 752, 1229
BshNI GGYRCC 2 cut(s) 392, 544
BsiHKAI GWGCWC 1 cut(s) 1042
BsiHKCI CYCGRG 2 cut(s) 1466, 1608
BsiSI CCGG 5 cut(s) 112, 259, 278, 503, 1434
BslFI GGGAC 2 cut(s) 494, 1744
BslI CCNNNNNNNGG 4 cut(s) 137, 284, 784, 1594
BsmAI GTCTC 1 cut(s) 33
BsmBI CGTCTC 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 494, 1744
BsmI GAATGC 1 cut(s) 1339
BsnI GGCC 4 cut(s) 438, 691, 752, 1229
BsoBI CYCGRG 2 cut(s) 1466, 1608
Bsp1286I GDGCHC 1 cut(s) 1042
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
Bsp1720I GCTNAGC 3 cut(s) 248, 1041, 1245
Bsp19I CCATGG 1 cut(s) 1726
BspACI CCGC 9 cut(s) 396, 439, 455, 490, 585, 1170, 1723, 1921, 2042
BspANI GGCC 4 cut(s) 438, 691, 752, 1229
BspCNI CTCAG 8 cut(s) 93, 589, 840, 1054, 1237, 1465, 2005, 2101
BspFNI CGCG 1 cut(s) 2042
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 7 cut(s) 276, 394, 546, 776, 1056, 1432, 1874
BspMAI CTGCAG 1 cut(s) 1484
BspPI GGATC 7 cut(s) 100, 250, 986, 1049, 1062, 1867, 1880
BspQI GCTCTTC 2 cut(s) 181, 1699
BspT107I GGYRCC 2 cut(s) 392, 544
BsrDI GCAATG 1 cut(s) 1843
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 385, 2027
BssECI CCNNGG 4 cut(s) 408, 513, 1726, 1942
BssMI GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
BssNI GRCGYC 1 cut(s) 545
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 3 cut(s) 408, 1726, 1942
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 4 cut(s) 355, 549, 1665, 1845
Bst4CI ACNGT 4 cut(s) 123, 267, 946, 1564
Bst6I CTCTTC 4 cut(s) 181, 550, 1699, 1818
BstACI GRCGYC 1 cut(s) 545
BstAPI GCANNNNNTGC 1 cut(s) 1445
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 3 cut(s) 492, 793, 1690
BstDSI CCRYGG 2 cut(s) 513, 1726
BstF5I GGATG 3 cut(s) 673, 793, 1684
BstFNI CGCG 1 cut(s) 2042
BstH2I RGCGCY 2 cut(s) 548, 1527
BstHHI GCGC 3 cut(s) 143, 547, 1526
BstKTI GATC 7 cut(s) 108, 258, 961, 981, 1057, 1639, 1875
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
BstMWI GCNNNNNNNGC 8 cut(s) 168, 171, 359, 395, 593, 1037, 1292, 1445
BstNI CCWGG 4 cut(s) 355, 549, 1665, 1845
BstNSI RCATGY 1 cut(s) 1204
BstSCI CCNGG 6 cut(s) 277, 353, 502, 547, 1663, 1843
BstSFI CTRYAG 1 cut(s) 1480
BstUI CGCG 1 cut(s) 2042
BstV2I GAAGAC 2 cut(s) 189, 1863
BstX2I RGATCY 2 cut(s) 1054, 1872
BstXI CCANNNNNNTGG 1 cut(s) 1868
BstYI RGATCY 2 cut(s) 1054, 1872
BsuI GTATCC 2 cut(s) 711, 1399
BsuRI GGCC 4 cut(s) 438, 691, 752, 1229
BtgI CCRYGG 2 cut(s) 513, 1726
BtgZI GCGATG 2 cut(s) 322, 873
BtsCI GGATG 3 cut(s) 673, 793, 1684
BtsI GCAGTG 1 cut(s) 2099
BtsIMutI CAGTG 1 cut(s) 2099
Cac8I GCNNGC 3 cut(s) 492, 793, 1690
CciI TCATGA 1 cut(s) 631
CfoI GCGC 3 cut(s) 143, 547, 1526
Cfr13I GGNCC 1 cut(s) 1772
CseI GACGC 1 cut(s) 302
Csp6I GTAC 6 cut(s) 393, 649, 883, 1086, 1565, 1659
CviQI GTAC 6 cut(s) 393, 649, 883, 1086, 1565, 1659
DinI GGCGCC 1 cut(s) 546
DpnI GATC 7 cut(s) 107, 257, 960, 980, 1056, 1638, 1874
DpnII GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 4 cut(s) 181, 550, 1699, 1818
EarI CTCTTC 4 cut(s) 181, 550, 1699, 1818
EciI GGCGGA 2 cut(s) 470, 574
Ecl136II GAGCTC 1 cut(s) 1040
Eco130I CCWWGG 3 cut(s) 408, 1726, 1942
Eco24I GRGCYC 1 cut(s) 1042
Eco47I GGWCC 1 cut(s) 1772
Eco53kI GAGCTC 1 cut(s) 1040
Eco57I CTGAAG 1 cut(s) 434
Eco88I CYCGRG 2 cut(s) 1466, 1608
EcoICRI GAGCTC 1 cut(s) 1040
EcoO109I RGGNCCY 1 cut(s) 1772
EcoRI GAATTC 2 cut(s) 1264, 1376
EcoRII CCWGG 4 cut(s) 353, 547, 1663, 1843
EcoT14I CCWWGG 3 cut(s) 408, 1726, 1942
EcoT22I ATGCAT 1 cut(s) 1867
EcoT38I GRGCYC 1 cut(s) 1042
EgeI GGCGCC 1 cut(s) 546
EheI GGCGCC 1 cut(s) 546
ErhI CCWWGG 3 cut(s) 408, 1726, 1942
Esp3I CGTCTC 1 cut(s) 33
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 2 cut(s) 494, 1744
FauI CCCGC 1 cut(s) 483
FbaI TGATCA 1 cut(s) 1636
FblI GTMKAC 2 cut(s) 237, 1491
FokI GGATG 3 cut(s) 680, 800, 1691
FriOI GRGCYC 1 cut(s) 1042
FspBI CTAG 3 cut(s) 156, 474, 1292
GlaI GCGC 3 cut(s) 142, 546, 1525
GsuI CTGGAG 1 cut(s) 1150
HaeII RGCGCY 2 cut(s) 548, 1527
HaeIII GGCC 4 cut(s) 438, 691, 752, 1229
HapII CCGG 5 cut(s) 112, 259, 278, 503, 1434
HgaI GACGC 1 cut(s) 302
HhaI GCGC 3 cut(s) 143, 547, 1526
Hin1I GRCGYC 1 cut(s) 545
Hin6I GCGC 3 cut(s) 141, 545, 1524
HinP1I GCGC 3 cut(s) 141, 545, 1524
HincII GTYRAC 3 cut(s) 238, 907, 2020
HindII GTYRAC 3 cut(s) 238, 907, 2020
HindIII AAGCTT 3 cut(s) 245, 1411, 1955
HpaII CCGG 5 cut(s) 112, 259, 278, 503, 1434
HphI GGTGA 5 cut(s) 397, 864, 964, 1546, 2055
Hpy166II GTNNAC 7 cut(s) 238, 643, 907, 1155, 1492, 1577, 2020
Hpy188I TCNGA 9 cut(s) 33, 105, 274, 327, 849, 894, 1877, 1977, 2008
Hpy188III TCNNGA 6 cut(s) 372, 632, 1048, 1373, 1634, 2101
Hpy8I GTNNAC 7 cut(s) 238, 643, 907, 1155, 1492, 1577, 2020
Hpy99I CGWCG 3 cut(s) 25, 242, 296
HpyCH4III ACNGT 4 cut(s) 123, 267, 946, 1564
HpyCH4IV ACGT 2 cut(s) 58, 1071
HpyF10VI GCNNNNNNNGC 8 cut(s) 168, 171, 359, 395, 593, 1037, 1292, 1445
HpySE526I ACGT 2 cut(s) 58, 1071
Hsp92I GRCGYC 1 cut(s) 545
HspAI GCGC 3 cut(s) 141, 545, 1524
KasI GGCGCC 1 cut(s) 544
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 1636
Kzo9I GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
LguI GCTCTTC 2 cut(s) 181, 1699
LmnI GCTCC 4 cut(s) 274, 855, 1037, 1169
LweI GCATC 6 cut(s) 307, 580, 778, 1231, 1270, 1894
MaeI CTAG 3 cut(s) 156, 474, 1292
MaeII ACGT 2 cut(s) 58, 1071
MaeIII GTNAC 4 cut(s) 59, 403, 940, 2033
MalI GATC 7 cut(s) 107, 257, 960, 980, 1056, 1638, 1874
MboI GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
MflI RGATCY 2 cut(s) 1054, 1872
MhlI GDGCHC 1 cut(s) 1042
Mly113I GGCGCC 1 cut(s) 545
MlyI GAGTC 3 cut(s) 516, 677, 1620
MmeI TCCRAC 2 cut(s) 265, 458
Mph1103I ATGCAT 1 cut(s) 1867
MseI TTAA 5 cut(s) 467, 843, 1143, 1233, 2028
MslI CAYNNNNRTG 2 cut(s) 1332, 1572
MspA1I CMGCKG 2 cut(s) 398, 1170
MspI CCGG 5 cut(s) 112, 259, 278, 503, 1434
MspR9I CCNGG 6 cut(s) 279, 355, 504, 549, 1665, 1845
Mva1269I GAATGC 1 cut(s) 1339
MvaI CCWGG 4 cut(s) 355, 549, 1665, 1845
MvnI CGCG 1 cut(s) 2042
MwoI GCNNNNNNNGC 8 cut(s) 168, 171, 359, 395, 593, 1037, 1292, 1445
NarI GGCGCC 1 cut(s) 545
NciI CCSGG 2 cut(s) 279, 504
NcoI CCATGG 1 cut(s) 1726
NdeII GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
NlaIV GGNNCC 7 cut(s) 276, 394, 546, 776, 1056, 1432, 1874
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 1867
NspI RCATGY 1 cut(s) 1204
PaeR7I CTCGAG 1 cut(s) 1608
PagI TCATGA 1 cut(s) 631
PciSI GCTCTTC 2 cut(s) 181, 1699
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 1 cut(s) 1339
PfeI GAWTC 9 cut(s) 4, 34, 208, 288, 481, 628, 998, 1339, 1885
PflMI CCANNNNNTGG 1 cut(s) 784
PfoI TCCNGGA 1 cut(s) 502
PleI GAGTC 3 cut(s) 515, 677, 1619
PluTI GGCGCC 1 cut(s) 548
PpsI GAGTC 3 cut(s) 515, 677, 1619
PpuMI RGGWCCY 1 cut(s) 1772
PsiI TTATAA 1 cut(s) 1461
Psp124BI GAGCTC 1 cut(s) 1042
Psp5II RGGWCCY 1 cut(s) 1772
Psp6I CCWGG 4 cut(s) 353, 547, 1663, 1843
PspGI CCWGG 4 cut(s) 353, 547, 1663, 1843
PspN4I GGNNCC 7 cut(s) 276, 394, 546, 776, 1056, 1432, 1874
PspPI GGNCC 1 cut(s) 1772
PspPPI RGGWCCY 1 cut(s) 1772
PspXI VCTCGAGB 1 cut(s) 1608
PstI CTGCAG 1 cut(s) 1484
PsuI RGATCY 2 cut(s) 1054, 1872
RsaI GTAC 6 cut(s) 394, 650, 884, 1087, 1566, 1660
RsaNI GTAC 6 cut(s) 393, 649, 883, 1086, 1565, 1659
RseI CAYNNNNRTG 2 cut(s) 1332, 1572
SacI GAGCTC 1 cut(s) 1042
SalI GTCGAC 1 cut(s) 236
SapI GCTCTTC 2 cut(s) 181, 1699
SaqAI TTAA 5 cut(s) 467, 843, 1143, 1233, 2028
Sau3AI GATC 7 cut(s) 105, 255, 958, 978, 1054, 1636, 1872
Sau96I GGNCC 1 cut(s) 1772
SchI GAGTC 3 cut(s) 516, 677, 1620
ScrFI CCNGG 6 cut(s) 279, 355, 504, 549, 1665, 1845
SduI GDGCHC 1 cut(s) 1042
SfaNI GCATC 6 cut(s) 307, 580, 778, 1231, 1270, 1894
SfcI CTRYAG 1 cut(s) 1480
SfoI GGCGCC 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 1608
SinI GGWCC 1 cut(s) 1772
SlaI CTCGAG 1 cut(s) 1608
SmiMI CAYNNNNRTG 2 cut(s) 1332, 1572
SmlI CTYRAG 3 cut(s) 685, 1273, 1608
SmoI CTYRAG 3 cut(s) 685, 1273, 1608
SsiI CCGC 9 cut(s) 396, 439, 455, 490, 585, 1170, 1723, 1921, 2042
SspDI GGCGCC 1 cut(s) 544
SspMI CTAG 3 cut(s) 156, 474, 1292
SstI GAGCTC 1 cut(s) 1042
StyD4I CCNGG 6 cut(s) 277, 353, 502, 547, 1663, 1843
StyI CCWWGG 3 cut(s) 408, 1726, 1942
TaaI ACNGT 4 cut(s) 123, 267, 946, 1564
TaiI ACGT 2 cut(s) 61, 1074
TaqI TCGA 8 cut(s) 237, 254, 291, 755, 981, 1115, 1353, 1609
TatI WGTACW 2 cut(s) 648, 1564
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 9 cut(s) 4, 34, 208, 288, 481, 628, 998, 1339, 1885
Tru1I TTAA 5 cut(s) 467, 843, 1143, 1233, 2028
Tru9I TTAA 5 cut(s) 467, 843, 1143, 1233, 2028
TscAI CASTG 1 cut(s) 2099
TseFI GTSAC 1 cut(s) 403
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 6 cut(s) 325, 1274, 1782, 1854, 1869, 1883
TspGWI ACGGA 1 cut(s) 1370
TspRI CASTG 1 cut(s) 2099
Van91I CCANNNNNTGG 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 1772
XapI RAATTY 3 cut(s) 1120, 1264, 1376
XceI RCATGY 1 cut(s) 1204
XhoI CTCGAG 1 cut(s) 1608
XmiI GTMKAC 2 cut(s) 237, 1491
XspI CTAG 3 cut(s) 156, 474, 1292
Zsp2I ATGCAT 1 cut(s) 1867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.