Rroxscaffold_6G00424650

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011079
Physical Location & Seq
Forward (+)
45782212 .. 45787407
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424650.1

Sequence Viewer

Length: 2205 bp
ATGCACTCCCTCTCATCCCATGCGACGCGTCCTCTCTCCTCTTCCTTCTCTCACTCCACCTCCTACTTCCGCTTCATCTCCCTCTCCCTCCCATTTCTCCACTCTCCCAAATCCCACCCCAAACTTTTCACTATTTCCCCAAAAGCCCCCACTTTCACGATCCGGAGCATCTCCGCCAAGCCCTCCTCGGAGCTCCGACCCAAGCGCCCCAATTCCGACTCCGACTCAAAGCTCCGAGCTCTCCGGGACCTCTTCTCCAAGCCCGGCGTCGGAATCGACGCTTACATCATTCCCTCTCAGGATGCTCACCAGAGTGAGTTCATTGCTGAATGTTATATGAGGAGGACCTATATATCGGGTTTCACTGGGAGTGCCGGCACTGCTGTTGTTACTAAGGATAAAGCAGCTCTTTGGACAGATGGGCGCTATTTTCTTCAGGCTGAAAAGCAGCTGAGTTCGAGTTGGATTCTCATGCGGGCTGGTAATTTGGGAGTACCTACAACTATTGAATGGCTGAATGATGTTTTGGCACCTGGTGGGAGAGTTGGCATTGACCCTGTGAGCAATACTTATTCTCTTAAAATGGGTGCTTCCGCTTATCTTTTGTACATGGATCAGTTTTTGTTTTCTTCCGATGCTGCAGAAGAATTGAAGCAGGGGATCGCTAACAAGAATCATGAGTTGGTTTTCCTGTATGATAAAAATCTTGTGGATGAAATATGGAAAGAATCAAGACCAGAGCCTCCAAAAAAACCAATTAGAGTACACGAACTGAAATATGCTGGTGCAGATGTGGCATCAAAGTTATCATATTTGAGGTCTGAACTTGTTGCAGCTGGTTCTTCTGCGATAGTTATATCTATGCTTGATGAAGTTGCCTGGCTGTTGAATTTGGTAATACTTCTGAACATCACTCTGGAACTGAGAGGAAGTGATGTTCCACATTCACCTGTTATGTATGCTTATCTAATTGTGGAGATCGACAAAGCAAAGTTATTTGTAGATACTTCCAGAGTAGGCACTGAGGTGTTGGATCACCTAAAGAATGCGGGTATTGAATTGAGGCCATATGACTCCATTCTTTCTGAAGTAGAAAGTCTGGCAGCAAAAGGGGCTAAAGTTTGGTTGGATGCATCATCTGTGAATGCAGCTATTGTCCACACCTACAACACTGCTTGTGAGAAATATTACGAGAGCCTTGAAAGTAAAAGAAAAAGTAAGACTAAGGAATATGATGACTCAAATGGTTGGTCTGAAGGACCCACTGGAATGTATAGGGTGTCCCCTGTCTCTTTGGCAAAGGCACTGAAAAATGATGCTGAGTTAGAAGGGATGCGGAGTTGTCATTTAAGAGATGCTGCTGCTTTAGCGCAGTTTTGGGTTTGGATCGAAGAGCAAATCAACAATAATGTGAAACTAACTGAGGTAGAAGCTGCAGAAAAACTTCTTGAATTTCGTTCAAGGCAGCCTGGTTTCCTAGACACAAGCTTTGACACCATCAGTGGCTCTGGTGCAAATGGTGCTATCATACATTATAAACCAGAACCAGATAGTTGCAGTGTTGTGAATGAAAAGAAACTCTTTCTGTTGGATAGTGGAGCTCAGTATGTGGACGGAACAACTGACATAACAAGGACGGTACATTTTGGTGAACCTTCTCCACGTCAAAAAGATTGCTTCACCCGAGTTTTACAGGGTCATATAGCTCTTGATCAGGCCGTATTCCCTGAAAACACACCGGGTTTTGTCTTAGATGCATTCGCTCGTTCTTTTCTTTGGAAGATTGGACTGGATTACCGACATGGAACCGGGCACGGTGTGGGAGCTGCATTAAATGTTCATGAGGGTCCTCAAAGTATTAGCTTTCGATTTGGAAATACAACTTCCTTACAGAGTGGTATGATTGTTAGCAATGAACCTGGCTATTATGAAGACCATGCCTTCGGCATTCGGATTGAGAATCTCCTTGTTGTGAAAGAAGTTGACACGCCAAATCGTTTTGGAGGAATTGGATACCTGGGATTTGAAAAACTCACATATGTTCCCATCCAGGCTAAATTGATAGACTTGTCGTTGCTTTCTGGTGCGGAATTTGATTGGCTTAATGATTATCATTCACAAGTTTGGGACAAGGTCTCACCATTGCTCGATGGTCCTGCACGCCAATGGCTTTGGAACAACACAAGACCACTTGTCAAGCAGTGA

Protein Analysis

734

Amino Acids

81.55

Weight (kDa)

6.16

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 80 - 192 4.6e-12 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 245 - 441 3.5e-40 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 443 - 660 1.5e-51 Metallopeptidase family M24
Peptidase_M24_C PF16188 671 - 731 6.4e-25 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AccB1I GGYRCC 1 cut(s) 529
AccII CGCG 1 cut(s) 28
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 7 cut(s) 70, 174, 475, 594, 1049, 1336, 2087
AclWI GGATC 5 cut(s) 154, 621, 668, 1041, 1394
AcsI RAATTY 3 cut(s) 889, 1451, 2090
AcuI CTGAAG 3 cut(s) 419, 1107, 1275
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 2 cut(s) 536, 1819
AfaI GTAC 4 cut(s) 495, 608, 765, 1641
AfiI CCNNNNNNNGG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 8 cut(s) 509, 652, 889, 1058, 1202, 1451, 1461, 2027
AhdI GACNNNNNGTC 1 cut(s) 2192
AjiI CACGTC 1 cut(s) 1664
AjnI CCWGG 6 cut(s) 532, 878, 1468, 1918, 2016, 2049
AjuI GAANNNNNNNTTGG 4 cut(s) 509, 541, 665, 697
AleI CACNNNNGTG 2 cut(s) 312, 1025
AloI GAACNNNNNNTCC 2 cut(s) 921, 953
Alw21I GWGCWC 3 cut(s) 195, 241, 1603
Alw26I GTCTC 2 cut(s) 1294, 2140
AlwI GGATC 5 cut(s) 154, 621, 668, 1041, 1394
Ama87I CYCGRG 1 cut(s) 1683
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 2 cut(s) 1064, 1716
ApoI RAATTY 3 cut(s) 889, 1451, 2090
ArsI GACNNNNNNTTYG 6 cut(s) 1235, 1267, 1472, 1504, 1925, 1957
Asp700I GAANNNNTTC 1 cut(s) 1443
AspLEI GCGC 3 cut(s) 207, 426, 1372
AspS9I GGNCC 5 cut(s) 247, 345, 1259, 1847, 2153
AsuC2I CCSGG 4 cut(s) 245, 264, 1740, 1810
AsuHPI GGTGA 6 cut(s) 299, 939, 1028, 1661, 1672, 2130
AvaI CYCGRG 1 cut(s) 1683
AvaII GGWCC 5 cut(s) 247, 345, 1259, 1847, 2153
BaeGI GKGCMC 1 cut(s) 1815
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 3 cut(s) 195, 241, 1603
BbsI GAAGAC 1 cut(s) 1938
Bbv12I GWGCWC 3 cut(s) 195, 241, 1603
BccI CCATC 4 cut(s) 413, 1505, 2054, 2144
BceAI ACGGC 1 cut(s) 1703
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciT130I CCWGG 6 cut(s) 534, 880, 1470, 1920, 2018, 2051
BciVI GTATCC 1 cut(s) 2006
BclI TGATCA 1 cut(s) 1711
BcnI CCSGG 4 cut(s) 245, 264, 1740, 1810
BcoDI GTCTC 2 cut(s) 1294, 2140
BfaI CTAG 1 cut(s) 1478
BfmI CTRYAG 2 cut(s) 639, 1434
BfoI RGCGCY 2 cut(s) 208, 427
BfuI GTATCC 1 cut(s) 2006
Bme18I GGWCC 5 cut(s) 247, 345, 1259, 1847, 2153
BmeRI GACNNNNNGTC 1 cut(s) 2192
BmeT110I CYCGRG 1 cut(s) 1683
BmgBI CACGTC 1 cut(s) 1664
BmgT120I GGNCC 5 cut(s) 247, 345, 1259, 1847, 2153
BmiI GGNNCC 5 cut(s) 248, 531, 1261, 1807, 1848
BmrI ACTGGG 1 cut(s) 375
BmuI ACTGGG 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 1938
BpuMI CCSGG 4 cut(s) 245, 264, 1740, 1810
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 2140
BsaJI CCNNGG 2 cut(s) 186, 2017
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 239, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 3 cut(s) 370, 1270, 1794
Bse3DI GCAATG 3 cut(s) 321, 1918, 2141
Bse8I GATNNNNATC 1 cut(s) 702
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 6 cut(s) 534, 880, 1470, 1920, 2018, 2051
BseDI CCNNGG 2 cut(s) 186, 2017
BseGI GGATG 6 cut(s) 14, 307, 718, 1135, 1338, 2046
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 3 cut(s) 321, 1918, 2141
BseMII CTCAG 7 cut(s) 311, 443, 914, 1014, 1311, 1413, 1616
BseNI ACTGG 3 cut(s) 370, 1270, 1794
BseRI GAGGAG 3 cut(s) 28, 175, 355
BseSI GKGCMC 1 cut(s) 1815
BsgI GTGCAG 2 cut(s) 807, 2142
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 1066, 1718
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 3 cut(s) 195, 241, 1603
BsiHKCI CYCGRG 1 cut(s) 1683
BsiSI CCGG 6 cut(s) 163, 244, 264, 375, 1739, 1809
BslFI GGGAC 3 cut(s) 260, 1267, 2141
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 2 cut(s) 1294, 2140
BsmFI GGGAC 3 cut(s) 260, 1267, 2141
BsmI GAATGC 4 cut(s) 1051, 1150, 1757, 1947
BsnI GGCC 2 cut(s) 1066, 1718
Bso31I GGTCTC 1 cut(s) 2140
BsoBI CYCGRG 1 cut(s) 1683
Bsp1286I GDGCHC 4 cut(s) 195, 241, 1603, 1815
Bsp13I TCCGGA 1 cut(s) 162
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
BspACI CCGC 7 cut(s) 70, 174, 475, 594, 1049, 1336, 2087
BspANI GGCC 2 cut(s) 1066, 1718
BspCNI CTCAG 7 cut(s) 310, 444, 915, 1015, 1312, 1414, 1615
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 1 cut(s) 28
BspHI TCATGA 2 cut(s) 676, 1840
BspLI GGNNCC 5 cut(s) 248, 531, 1261, 1807, 1848
BspMAI CTGCAG 2 cut(s) 643, 1438
BspPI GGATC 5 cut(s) 154, 621, 668, 1041, 1394
BspQI GCTCTTC 1 cut(s) 1386
BspT107I GGYRCC 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 2140
BsrDI GCAATG 3 cut(s) 321, 1918, 2141
BsrFI RCCGGY 1 cut(s) 374
BsrGI TGTACA 1 cut(s) 606
BsrI ACTGG 3 cut(s) 370, 1270, 1794
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 186, 2017
BssMI GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 6 cut(s) 534, 880, 1470, 1920, 2018, 2051
Bst4CI ACNGT 2 cut(s) 1639, 1817
Bst6I CTCTTC 3 cut(s) 46, 257, 1386
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 376, 477, 2161
BstF5I GGATG 6 cut(s) 14, 307, 718, 1135, 1338, 2046
BstFNI CGCG 1 cut(s) 28
BstH2I RGCGCY 2 cut(s) 208, 427
BstHHI GCGC 3 cut(s) 207, 426, 1372
BstKTI GATC 7 cut(s) 162, 616, 663, 981, 1036, 1389, 1714
BstMAI GTCTC 2 cut(s) 1294, 2140
BstMBI GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
BstMWI GCNNNNNNNGC 5 cut(s) 380, 794, 1112, 1367, 1520
BstNI CCWGG 6 cut(s) 534, 880, 1470, 1920, 2018, 2051
BstSFI CTRYAG 2 cut(s) 639, 1434
BstSLI GKGCMC 1 cut(s) 1815
BstUI CGCG 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 1938
BsuI GTATCC 1 cut(s) 2006
BsuRI GGCC 2 cut(s) 1066, 1718
BtrI CACGTC 1 cut(s) 1664
BtsCI GGATG 6 cut(s) 14, 307, 718, 1135, 1338, 2046
BtsI GCAGTG 3 cut(s) 378, 1170, 1564
BtsIMutI CAGTG 8 cut(s) 363, 378, 1020, 1170, 1263, 1304, 1507, 1564
Cac8I GCNNGC 3 cut(s) 376, 477, 2161
CciI TCATGA 2 cut(s) 676, 1840
CfoI GCGC 3 cut(s) 207, 426, 1372
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 5 cut(s) 247, 345, 1259, 1847, 2153
CseI GACGC 4 cut(s) 17, 34, 256, 287
CsiI ACCWGGT 1 cut(s) 532
Csp6I GTAC 4 cut(s) 494, 607, 764, 1640
CviAII CATG 7 cut(s) 20, 472, 610, 677, 1802, 1841, 1937
CviQI GTAC 4 cut(s) 494, 607, 764, 1640
DpnI GATC 7 cut(s) 161, 615, 662, 980, 1035, 1388, 1713
DpnII GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
DraIII CACNNNGTG 2 cut(s) 536, 1819
DriI GACNNNNNGTC 1 cut(s) 2192
Eam1104I CTCTTC 3 cut(s) 46, 257, 1386
Eam1105I GACNNNNNGTC 1 cut(s) 2192
EarI CTCTTC 3 cut(s) 46, 257, 1386
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 3 cut(s) 193, 239, 1601
Eco24I GRGCYC 3 cut(s) 195, 241, 1603
Eco31I GGTCTC 1 cut(s) 2140
Eco47I GGWCC 5 cut(s) 247, 345, 1259, 1847, 2153
Eco53kI GAGCTC 3 cut(s) 193, 239, 1601
Eco57I CTGAAG 3 cut(s) 419, 1107, 1275
Eco88I CYCGRG 1 cut(s) 1683
EcoICRI GAGCTC 3 cut(s) 193, 239, 1601
EcoO109I RGGNCCY 4 cut(s) 247, 345, 1259, 1847
EcoRII CCWGG 6 cut(s) 532, 878, 1468, 1918, 2016, 2049
EcoT22I ATGCAT 2 cut(s) 1135, 1759
EcoT38I GRGCYC 3 cut(s) 195, 241, 1603
FaeI CATG 7 cut(s) 23, 475, 613, 680, 1805, 1844, 1940
FalI AAGNNNNNCTT 4 cut(s) 393, 425, 1565, 1597
FaqI GGGAC 3 cut(s) 260, 1267, 2141
FatI CATG 7 cut(s) 19, 471, 609, 676, 1801, 1840, 1936
FauI CCCGC 2 cut(s) 468, 1042
FauNDI CATATG 2 cut(s) 1069, 2038
FbaI TGATCA 1 cut(s) 1711
FokI GGATG 5 cut(s) 314, 725, 1142, 1345, 2033
FriOI GRGCYC 3 cut(s) 195, 241, 1603
FspBI CTAG 1 cut(s) 1478
GlaI GCGC 3 cut(s) 206, 425, 1371
HaeII RGCGCY 2 cut(s) 208, 427
HaeIII GGCC 2 cut(s) 1066, 1718
HapII CCGG 6 cut(s) 163, 244, 264, 375, 1739, 1809
HgaI GACGC 4 cut(s) 17, 34, 256, 287
HhaI GCGC 3 cut(s) 207, 426, 1372
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 7 cut(s) 23, 475, 613, 680, 1805, 1844, 1940
Hin6I GCGC 3 cut(s) 205, 424, 1370
HinP1I GCGC 3 cut(s) 205, 424, 1370
HincII GTYRAC 1 cut(s) 1984
HindII GTYRAC 1 cut(s) 1984
HindIII AAGCTT 1 cut(s) 1486
HinfI GANTC 9 cut(s) 218, 224, 273, 466, 673, 728, 1073, 1238, 1960
HpaII CCGG 6 cut(s) 163, 244, 264, 375, 1739, 1809
HphI GGTGA 6 cut(s) 299, 939, 1028, 1661, 1672, 2130
Hpy166II GTNNAC 5 cut(s) 766, 1159, 1612, 1652, 1984
Hpy8I GTNNAC 5 cut(s) 766, 1159, 1612, 1652, 1984
Hpy99I CGWCG 3 cut(s) 28, 272, 281
HpyAV CCTTC 5 cut(s) 55, 1250, 1322, 1665, 1951
HpyCH4III ACNGT 2 cut(s) 1639, 1817
HpyCH4IV ACGT 1 cut(s) 1663
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 794, 1112, 1367, 1520
HpySE526I ACGT 1 cut(s) 1663
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 7 cut(s) 23, 475, 613, 680, 1805, 1844, 1940
HspAI GCGC 3 cut(s) 205, 424, 1370
Kpn2I TCCGGA 1 cut(s) 162
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1711
Kzo9I GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
LguI GCTCTTC 1 cut(s) 1386
LmnI GCTCC 6 cut(s) 165, 190, 198, 237, 1598, 1823
MabI ACCWGGT 1 cut(s) 532
MaeI CTAG 1 cut(s) 1478
MaeII ACGT 1 cut(s) 1663
MaeIII GTNAC 1 cut(s) 388
MalI GATC 7 cut(s) 161, 615, 662, 980, 1035, 1388, 1713
MboI GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
MboII GAAGA 9 cut(s) 33, 244, 425, 621, 656, 834, 1403, 1792, 1943
MhlI GDGCHC 4 cut(s) 195, 241, 1603, 1815
MluI ACGCGT 1 cut(s) 26
MlyI GAGTC 4 cut(s) 212, 218, 1067, 1232
MmeI TCCRAC 8 cut(s) 220, 240, 246, 250, 443, 1011, 1107, 1569
Mph1103I ATGCAT 2 cut(s) 1135, 1759
MroI TCCGGA 1 cut(s) 162
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 1443
MseI TTAA 4 cut(s) 579, 1349, 1832, 2103
MslI CAYNNNNRTG 5 cut(s) 312, 1025, 1268, 1647, 2164
MspA1I CMGCKG 2 cut(s) 451, 836
MspI CCGG 6 cut(s) 163, 244, 264, 375, 1739, 1809
Mva1269I GAATGC 4 cut(s) 1051, 1150, 1757, 1947
MvaI CCWGG 6 cut(s) 534, 880, 1470, 1920, 2018, 2051
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 5 cut(s) 380, 794, 1112, 1367, 1520
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 4 cut(s) 245, 264, 1740, 1810
NdeI CATATG 2 cut(s) 1069, 2038
NdeII GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 7 cut(s) 23, 475, 613, 680, 1805, 1844, 1940
NlaIV GGNNCC 5 cut(s) 248, 531, 1261, 1807, 1848
NsiI ATGCAT 2 cut(s) 1135, 1759
OliI CACNNNNGTG 2 cut(s) 312, 1025
PagI TCATGA 2 cut(s) 676, 1840
PciSI GCTCTTC 1 cut(s) 1386
PctI GAATGC 4 cut(s) 1051, 1150, 1757, 1947
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 1443
PfeI GAWTC 5 cut(s) 273, 466, 673, 728, 1960
PflFI GACNNNGTC 1 cut(s) 2132
PfoI TCCNGGA 1 cut(s) 243
PleI GAGTC 4 cut(s) 212, 218, 1067, 1232
PpsI GAGTC 4 cut(s) 212, 218, 1067, 1232
PpuMI RGGWCCY 4 cut(s) 247, 345, 1259, 1847
PsiI TTATAA 1 cut(s) 1536
Psp124BI GAGCTC 3 cut(s) 195, 241, 1603
Psp5II RGGWCCY 4 cut(s) 247, 345, 1259, 1847
Psp6I CCWGG 6 cut(s) 532, 878, 1468, 1918, 2016, 2049
PspGI CCWGG 6 cut(s) 532, 878, 1468, 1918, 2016, 2049
PspN4I GGNNCC 5 cut(s) 248, 531, 1261, 1807, 1848
PspPI GGNCC 5 cut(s) 247, 345, 1259, 1847, 2153
PspPPI RGGWCCY 4 cut(s) 247, 345, 1259, 1847
PstI CTGCAG 2 cut(s) 643, 1438
PsyI GACNNNGTC 1 cut(s) 2132
PvuII CAGCTG 2 cut(s) 451, 836
RsaI GTAC 4 cut(s) 495, 608, 765, 1641
RsaNI GTAC 4 cut(s) 494, 607, 764, 1640
RseI CAYNNNNRTG 5 cut(s) 312, 1025, 1268, 1647, 2164
SacI GAGCTC 3 cut(s) 195, 241, 1603
SapI GCTCTTC 1 cut(s) 1386
SaqAI TTAA 4 cut(s) 579, 1349, 1832, 2103
Sau3AI GATC 7 cut(s) 159, 613, 660, 978, 1033, 1386, 1711
Sau96I GGNCC 5 cut(s) 247, 345, 1259, 1847, 2153
SchI GAGTC 4 cut(s) 212, 218, 1067, 1232
SduI GDGCHC 4 cut(s) 195, 241, 1603, 1815
SexAI ACCWGGT 1 cut(s) 532
SfcI CTRYAG 2 cut(s) 639, 1434
SinI GGWCC 5 cut(s) 247, 345, 1259, 1847, 2153
SmiMI CAYNNNNRTG 5 cut(s) 312, 1025, 1268, 1647, 2164
SsiI CCGC 7 cut(s) 70, 174, 475, 594, 1049, 1336, 2087
SspI AATATT 1 cut(s) 1187
SspMI CTAG 1 cut(s) 1478
SstI GAGCTC 3 cut(s) 195, 241, 1603
TaaI ACNGT 2 cut(s) 1639, 1817
TaiI ACGT 1 cut(s) 1666
TaqI TCGA 6 cut(s) 276, 458, 981, 1389, 1867, 2148
TatI WGTACW 2 cut(s) 606, 763
TfiI GAWTC 5 cut(s) 273, 466, 673, 728, 1960
Tru1I TTAA 4 cut(s) 579, 1349, 1832, 2103
Tru9I TTAA 4 cut(s) 579, 1349, 1832, 2103
TscAI CASTG 8 cut(s) 370, 385, 1027, 1177, 1270, 1311, 1507, 1564
TspDTI ATGAA 8 cut(s) 64, 310, 729, 885, 1584, 1829, 1929, 1944
TspGWI ACGGA 1 cut(s) 1629
TspRI CASTG 8 cut(s) 370, 385, 1027, 1177, 1270, 1311, 1507, 1564
Tth111I GACNNNGTC 1 cut(s) 2132
VpaK11BI GGWCC 5 cut(s) 247, 345, 1259, 1847, 2153
XapI RAATTY 3 cut(s) 889, 1451, 2090
XmnI GAANNNNTTC 1 cut(s) 1443
XspI CTAG 1 cut(s) 1478
Zsp2I ATGCAT 2 cut(s) 1135, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.