Rh3AG063100

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011079
Physical Location & Seq
Reverse (-)
4356727 .. 4361038
4312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG063100.1

Sequence Viewer

Length: 1779 bp
ATGAGGAGGACCTATATATCGGGTTTCACAGGGAGTGCTGGCACTGCTGTTGTCACTAAGGATAAAGCAGCTCTTTGGACAGATGGCCGCTATTTTCTTCAGGCTGAAAAGCAGCTGAGTTCGAGTTGGATTCTCATGCGGGCTGGTAATTTTGGAGTACCTACAACTATTGAATGGCTGAATGATGTTTTGGCCCCTGGTGGGAGAGTTGGCATTGACCCTTTTCTGTTTTCTTCCGATGCTGCAGAAGAATTGAAGCAGGGCATCGCTAACAAGAATCATGAGTTGGTTTTTCTGTATGATAAAAATCTTGTGGATGAAATATGGAAAGAATCAAGACCAGAGCCTCCAAAAAAACCAATTAGAGTACACGAACTGAAATATGCTGGTGCAGATGTGGCATCAAAGTTATCATCTTTGAGGTCTGAACTTGTAGCAGCTGGTTCATATGCGATAGTTATATCTATGCTTGATGAAGTTGCCTGGCTGTTGAATTTGAGAGGAAGTGATGTTCCACATTCACCTGTTATGTATGCTTATCTGATTGTGGAGATCGACAAAGCAAAGTTATTTGTAGATACTTCCAAAGTAGGCACTGAGGTGTTGGATCACCTGGAGAATGCGGGTATTGAATTGAGGCCGTATGACTCCATTCTTTCTGAAGTAGAAAGTCTGGCAGCAAAAGGGGCTAAAGTTTGGTTGGATGCATCATCTGTGAATGCAGCTATTGTCCACACCTACAACACTGCTTGTGAGAAATATTATGAGAGCCTTGAAAGTAAAAGAAAAAGTAAGACTAAGGAATATGATGACTCAAATGGTTGGTCTGAAGGACCCACTGGAATGTATAGGGTGTCCCCTGTCTCTTTGGCAAAGGCACTGAAAAATGATGCTGAGTTAGAAGGGATGCGGAGTTGTCATTTAAGGGATGCTGCTGCTTTAGCGCAGTTTTGGGTTTGGATTGAAGAGCAAATCAACAAGAATGTGAAACTAACTGAGGTAGAAGCTGCAGAAAAACTTCTTGAATTTCGTTCAAGGCAGCCTGGTTTCCTAGATACAAGCTTTGACACCATCAGTGGCTCTGGTGCAAATGGTGCTATCATACATTATAAACCAGAACCAGATAGTTGCAGTGTTGTGAATGAAAGGAAACTCTTTCTGTTGGATAGTGGAGCTCAGTATGTGGACGGAACAACTGACATAACAAGGACCGTACATTTTGGTGAACCTTCTCCACGTCAAAAAGACTGCTTCACCCGAGTTTTACAGGGTCATATAGCTCTTGATCAGGCCGTATTCCCTGAAAACACACCAGGTTTTGTCTTAGATGCATTTGCTCGTTCTTTTCTTTGGAAGATTGGACTGGATTACCGACATGGAACTGGGCACGGTGTGGGAGCTGCATTAAATGTTCATGAGGGTCCTCAAAGTATTAGCTTTCGATTTGGAAATACAACTTCCTTACAGAGTGGTATGATTGTTAGCAATGAACCTGGCTATTATGAAGACCATGCCTTCGGCATTCGGATTGAGAATCTCCTTGTTGTGAAAGAAGTTGACACGCCAAATCGTTTTGGAGGAATTGGATACCTGGGATTTGAAAAACTCACATATGTTCCCATCCAGGCTAAATTGATAGACTTGTCTTTGCTTTCTGGTGCGGAATTTGATTGGCTTAATGATTATCATTCACAAGTTTGGGACAAGGTCTCACCATTGCTCGATGGTCCTGCACGCCAATGGCTTTGGAACAACACAAGACCACTTGTCAAGCAGTGA

Protein Analysis

592

Amino Acids

65.78

Weight (kDa)

5.51

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 3 - 92 4e-12 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 113 - 299 6.2e-46 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 301 - 518 1.5e-51 Metallopeptidase family M24
Peptidase_M24_C PF16188 529 - 589 4.8e-25 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
AciI CCGC 5 cut(s) 88, 139, 623, 910, 1661
AclWI GGATC 1 cut(s) 615
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 3 cut(s) 493, 1025, 1664
AcuI CTGAAG 3 cut(s) 83, 681, 849
AdeI CACNNNGTG 1 cut(s) 1393
AfaI GTAC 3 cut(s) 159, 369, 1215
AfiI CCNNNNNNNGG 1 cut(s) 201
AgsI TTSAA 9 cut(s) 173, 256, 493, 632, 776, 965, 1025, 1035, 1601
AhdI GACNNNNNGTC 1 cut(s) 1766
AjiI CACGTC 1 cut(s) 1238
AjnI CCWGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 269, 301
AleI CACNNNNGTG 1 cut(s) 599
AloI GAACNNNNNNTCC 2 cut(s) 495, 527
Alw21I GWGCWC 1 cut(s) 1177
Alw26I GTCTC 2 cut(s) 868, 1714
AlwI GGATC 1 cut(s) 615
Ama87I CYCGRG 1 cut(s) 1257
AoxI GGCC 4 cut(s) 85, 192, 638, 1290
ApoI RAATTY 3 cut(s) 493, 1025, 1664
ArsI GACNNNNNNTTYG 4 cut(s) 809, 841, 1499, 1531
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 6 cut(s) 9, 193, 833, 1209, 1421, 1727
AsuHPI GGTGA 5 cut(s) 513, 602, 1235, 1246, 1704
AvaI CYCGRG 1 cut(s) 1257
AvaII GGWCC 5 cut(s) 9, 833, 1209, 1421, 1727
BaeGI GKGCMC 1 cut(s) 1389
BanII GRGCYC 1 cut(s) 1177
BbsI GAAGAC 1 cut(s) 1512
Bbv12I GWGCWC 1 cut(s) 1177
BccI CCATC 4 cut(s) 77, 1079, 1628, 1718
BceAI ACGGC 2 cut(s) 625, 1277
BcgI CGANNNNNNTGC 2 cut(s) 1712, 1746
BciT130I CCWGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
BciVI GTATCC 1 cut(s) 1580
BclI TGATCA 1 cut(s) 1285
BcoDI GTCTC 2 cut(s) 868, 1714
BfaI CTAG 1 cut(s) 1052
BfmI CTRYAG 2 cut(s) 243, 1008
BfuI GTATCC 1 cut(s) 1580
Bme1390I CCNGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
Bme18I GGWCC 5 cut(s) 9, 833, 1209, 1421, 1727
BmeRI GACNNNNNGTC 1 cut(s) 1766
BmeT110I CYCGRG 1 cut(s) 1257
BmgBI CACGTC 1 cut(s) 1238
BmgT120I GGNCC 6 cut(s) 9, 193, 833, 1209, 1421, 1727
BmiI GGNNCC 3 cut(s) 195, 835, 1422
BmrFI CCNGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
BmrI ACTGGG 1 cut(s) 1392
BmsI GCATC 9 cut(s) 229, 273, 410, 694, 716, 880, 897, 919, 1318
BmuI ACTGGG 1 cut(s) 1392
BpiI GAAGAC 1 cut(s) 1512
BpmI CTGGAG 1 cut(s) 635
BsaBI GATNNNNATC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 1714
BsaJI CCNNGG 2 cut(s) 196, 1591
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse1I ACTGG 3 cut(s) 844, 1368, 1387
Bse3DI GCAATG 2 cut(s) 1492, 1715
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
BseDI CCNNGG 2 cut(s) 196, 1591
BseGI GGATG 5 cut(s) 322, 709, 912, 934, 1620
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMI GCAATG 2 cut(s) 1492, 1715
BseMII CTCAG 5 cut(s) 107, 588, 885, 987, 1190
BseNI ACTGG 3 cut(s) 844, 1368, 1387
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 1389
BsgI GTGCAG 2 cut(s) 411, 1716
BshFI GGCC 4 cut(s) 87, 194, 640, 1292
BsiHKAI GWGCWC 1 cut(s) 1177
BsiHKCI CYCGRG 1 cut(s) 1257
BslFI GGGAC 2 cut(s) 841, 1715
BslI CCNNNNNNNGG 1 cut(s) 201
BsmAI GTCTC 2 cut(s) 868, 1714
BsmFI GGGAC 2 cut(s) 841, 1715
BsmI GAATGC 3 cut(s) 625, 724, 1521
BsnI GGCC 4 cut(s) 87, 194, 640, 1292
Bso31I GGTCTC 1 cut(s) 1714
BsoBI CYCGRG 1 cut(s) 1257
Bsp1286I GDGCHC 2 cut(s) 1177, 1389
Bsp143I GATC 3 cut(s) 552, 607, 1285
BspACI CCGC 5 cut(s) 88, 139, 623, 910, 1661
BspANI GGCC 4 cut(s) 87, 194, 640, 1292
BspCNI CTCAG 5 cut(s) 108, 589, 886, 988, 1189
BspHI TCATGA 2 cut(s) 280, 1414
BspLI GGNNCC 3 cut(s) 195, 835, 1422
BspMAI CTGCAG 2 cut(s) 247, 1012
BspPI GGATC 1 cut(s) 615
BspQI GCTCTTC 1 cut(s) 960
BspTNI GGTCTC 1 cut(s) 1714
BsrDI GCAATG 2 cut(s) 1492, 1715
BsrI ACTGG 3 cut(s) 844, 1368, 1387
BssECI CCNNGG 2 cut(s) 196, 1591
BssMI GATC 3 cut(s) 552, 607, 1285
Bst2UI CCWGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
Bst4CI ACNGT 2 cut(s) 1213, 1391
Bst6I CTCTTC 1 cut(s) 960
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstC8I GCNNGC 3 cut(s) 40, 141, 1735
BstDEI CTNAG 8 cut(s) 57, 116, 597, 798, 894, 996, 1176, 1324
BstF5I GGATG 5 cut(s) 322, 709, 912, 934, 1620
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 3 cut(s) 555, 610, 1288
BstMAI GTCTC 2 cut(s) 868, 1714
BstMBI GATC 3 cut(s) 552, 607, 1285
BstMWI GCNNNNNNNGC 5 cut(s) 44, 398, 686, 941, 1094
BstNI CCWGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
BstSCI CCNGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
BstSFI CTRYAG 2 cut(s) 243, 1008
BstSLI GKGCMC 1 cut(s) 1389
BstV2I GAAGAC 1 cut(s) 1512
BsuI GTATCC 1 cut(s) 1580
BsuRI GGCC 4 cut(s) 87, 194, 640, 1292
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 1238
BtsCI GGATG 5 cut(s) 322, 709, 912, 934, 1620
BtsI GCAGTG 3 cut(s) 42, 744, 1138
BtsIMutI CAGTG 7 cut(s) 42, 594, 744, 837, 878, 1081, 1138
Cac8I GCNNGC 3 cut(s) 40, 141, 1735
CciI TCATGA 2 cut(s) 280, 1414
CfoI GCGC 1 cut(s) 946
Cfr13I GGNCC 6 cut(s) 9, 193, 833, 1209, 1421, 1727
CsiI ACCWGGT 1 cut(s) 1312
Csp6I GTAC 3 cut(s) 158, 368, 1214
CviAII CATG 5 cut(s) 136, 281, 1376, 1415, 1511
CviQI GTAC 3 cut(s) 158, 368, 1214
DdeI CTNAG 8 cut(s) 57, 116, 597, 798, 894, 996, 1176, 1324
DpnI GATC 3 cut(s) 554, 609, 1287
DpnII GATC 3 cut(s) 552, 607, 1285
DraIII CACNNNGTG 1 cut(s) 1393
DriI GACNNNNNGTC 1 cut(s) 1766
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 1 cut(s) 960
Eam1105I GACNNNNNGTC 1 cut(s) 1766
EarI CTCTTC 1 cut(s) 960
Ecl136II GAGCTC 1 cut(s) 1175
Eco24I GRGCYC 1 cut(s) 1177
Eco31I GGTCTC 1 cut(s) 1714
Eco47I GGWCC 5 cut(s) 9, 833, 1209, 1421, 1727
Eco53kI GAGCTC 1 cut(s) 1175
Eco57I CTGAAG 3 cut(s) 83, 681, 849
Eco88I CYCGRG 1 cut(s) 1257
EcoICRI GAGCTC 1 cut(s) 1175
EcoO109I RGGNCCY 3 cut(s) 9, 833, 1421
EcoRII CCWGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
EcoT22I ATGCAT 2 cut(s) 709, 1333
EcoT38I GRGCYC 1 cut(s) 1177
FaeI CATG 5 cut(s) 139, 284, 1379, 1418, 1514
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 2 cut(s) 841, 1715
FatI CATG 5 cut(s) 135, 280, 1375, 1414, 1510
FauI CCCGC 2 cut(s) 132, 616
FauNDI CATATG 2 cut(s) 448, 1612
FbaI TGATCA 1 cut(s) 1285
FokI GGATG 5 cut(s) 329, 716, 919, 941, 1607
FriOI GRGCYC 1 cut(s) 1177
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 1 cut(s) 945
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 4 cut(s) 87, 194, 640, 1292
HhaI GCGC 1 cut(s) 946
Hin1II CATG 5 cut(s) 139, 284, 1379, 1418, 1514
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HincII GTYRAC 1 cut(s) 1558
HindII GTYRAC 1 cut(s) 1558
HindIII AAGCTT 1 cut(s) 1060
HinfI GANTC 6 cut(s) 130, 277, 332, 647, 812, 1534
HphI GGTGA 5 cut(s) 513, 602, 1235, 1246, 1704
Hpy166II GTNNAC 5 cut(s) 370, 733, 1186, 1226, 1558
Hpy188I TCNGA 6 cut(s) 238, 427, 543, 661, 829, 1527
Hpy188III TCNNGA 5 cut(s) 281, 336, 1022, 1283, 1415
Hpy8I GTNNAC 5 cut(s) 370, 733, 1186, 1226, 1558
HpyAV CCTTC 4 cut(s) 824, 896, 1239, 1525
HpyCH4III ACNGT 2 cut(s) 1213, 1391
HpyCH4IV ACGT 1 cut(s) 1237
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 398, 686, 941, 1094
HpyF3I CTNAG 8 cut(s) 57, 116, 597, 798, 894, 996, 1176, 1324
HpySE526I ACGT 1 cut(s) 1237
Hsp92II CATG 5 cut(s) 139, 284, 1379, 1418, 1514
HspAI GCGC 1 cut(s) 944
Ksp22I TGATCA 1 cut(s) 1285
Kzo9I GATC 3 cut(s) 552, 607, 1285
LguI GCTCTTC 1 cut(s) 960
LmnI GCTCC 2 cut(s) 1172, 1397
LweI GCATC 9 cut(s) 229, 273, 410, 694, 716, 880, 897, 919, 1318
MabI ACCWGGT 1 cut(s) 1312
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 1 cut(s) 1237
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 554, 609, 1287
MboI GATC 3 cut(s) 552, 607, 1285
MboII GAAGA 6 cut(s) 89, 225, 260, 977, 1366, 1517
MhlI GDGCHC 2 cut(s) 1177, 1389
MluCI AATT 9 cut(s) 148, 251, 360, 493, 632, 1025, 1581, 1631, 1664
MlyI GAGTC 2 cut(s) 641, 806
MmeI TCCRAC 4 cut(s) 107, 585, 681, 1143
MnlI CCTC 9 cut(s) 357, 414, 494, 592, 630, 991, 1411, 1434, 1571
Mph1103I ATGCAT 2 cut(s) 709, 1333
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 3 cut(s) 923, 1406, 1677
MslI CAYNNNNRTG 4 cut(s) 599, 842, 1221, 1738
MspA1I CMGCKG 2 cut(s) 115, 440
MspR9I CCNGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
Mva1269I GAATGC 3 cut(s) 625, 724, 1521
MvaI CCWGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
MwoI GCNNNNNNNGC 5 cut(s) 44, 398, 686, 941, 1094
NdeI CATATG 2 cut(s) 448, 1612
NdeII GATC 3 cut(s) 552, 607, 1285
NlaIII CATG 5 cut(s) 139, 284, 1379, 1418, 1514
NlaIV GGNNCC 3 cut(s) 195, 835, 1422
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 2 cut(s) 709, 1333
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 2 cut(s) 280, 1414
PciSI GCTCTTC 1 cut(s) 960
PctI GAATGC 3 cut(s) 625, 724, 1521
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 4 cut(s) 130, 277, 332, 1534
PflFI GACNNNGTC 1 cut(s) 1706
PleI GAGTC 2 cut(s) 641, 806
PpsI GAGTC 2 cut(s) 641, 806
PpuMI RGGWCCY 3 cut(s) 9, 833, 1421
PsiI TTATAA 1 cut(s) 1110
Psp124BI GAGCTC 1 cut(s) 1177
Psp5II RGGWCCY 3 cut(s) 9, 833, 1421
Psp6I CCWGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
PspGI CCWGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
PspN4I GGNNCC 3 cut(s) 195, 835, 1422
PspPI GGNCC 6 cut(s) 9, 193, 833, 1209, 1421, 1727
PspPPI RGGWCCY 3 cut(s) 9, 833, 1421
PstI CTGCAG 2 cut(s) 247, 1012
PsyI GACNNNGTC 1 cut(s) 1706
PvuII CAGCTG 2 cut(s) 115, 440
RsaI GTAC 3 cut(s) 159, 369, 1215
RsaNI GTAC 3 cut(s) 158, 368, 1214
RseI CAYNNNNRTG 4 cut(s) 599, 842, 1221, 1738
SacI GAGCTC 1 cut(s) 1177
SapI GCTCTTC 1 cut(s) 960
SaqAI TTAA 3 cut(s) 923, 1406, 1677
Sau3AI GATC 3 cut(s) 552, 607, 1285
Sau96I GGNCC 6 cut(s) 9, 193, 833, 1209, 1421, 1727
SchI GAGTC 2 cut(s) 641, 806
ScrFI CCNGG 8 cut(s) 198, 484, 614, 1044, 1314, 1494, 1592, 1625
SduI GDGCHC 2 cut(s) 1177, 1389
SexAI ACCWGGT 1 cut(s) 1312
SfaNI GCATC 9 cut(s) 229, 273, 410, 694, 716, 880, 897, 919, 1318
SfcI CTRYAG 2 cut(s) 243, 1008
SinI GGWCC 5 cut(s) 9, 833, 1209, 1421, 1727
SmiMI CAYNNNNRTG 4 cut(s) 599, 842, 1221, 1738
Sse9I AATT 9 cut(s) 148, 251, 360, 493, 632, 1025, 1581, 1631, 1664
SsiI CCGC 5 cut(s) 88, 139, 623, 910, 1661
SspI AATATT 1 cut(s) 761
SspMI CTAG 1 cut(s) 1052
SstI GAGCTC 1 cut(s) 1177
StyD4I CCNGG 8 cut(s) 196, 482, 612, 1042, 1312, 1492, 1590, 1623
TaaI ACNGT 2 cut(s) 1213, 1391
TaiI ACGT 1 cut(s) 1240
TaqI TCGA 4 cut(s) 122, 555, 1441, 1722
TasI AATT 9 cut(s) 148, 251, 360, 493, 632, 1025, 1581, 1631, 1664
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 4 cut(s) 130, 277, 332, 1534
Tru1I TTAA 3 cut(s) 923, 1406, 1677
Tru9I TTAA 3 cut(s) 923, 1406, 1677
TscAI CASTG 7 cut(s) 49, 601, 751, 844, 885, 1081, 1138
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 7 cut(s) 333, 435, 489, 1158, 1403, 1503, 1518
TspGWI ACGGA 1 cut(s) 1203
TspRI CASTG 7 cut(s) 49, 601, 751, 844, 885, 1081, 1138
Tth111I GACNNNGTC 1 cut(s) 1706
VpaK11BI GGWCC 5 cut(s) 9, 833, 1209, 1421, 1727
XapI RAATTY 3 cut(s) 493, 1025, 1664
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 1 cut(s) 1052
Zsp2I ATGCAT 2 cut(s) 709, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.