MD04G1205000.v1.1

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011079
Physical Location & Seq
Forward (+)
29109777 .. 29116074
6298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1205000.v1.1.491

Sequence Viewer

Length: 2262 bp
ATGAGATGGAGAGAAAGGGGAGACAAGAAACGACAAGTCGTTGTTAATCGCATCTTCTTCCCTCTCTGTGTGGTGACTGATAAGATAACTGAAGCTGTTCTTTCAACAACTCTTTCCTTCTCTCTCCTGAGTTTTGCTACGATGCACTCATTGTCATCACAGGCAGTGCGCCCTCTCTCCTTTCACTCCGCCCGCTCTCACTACTTCCGCCGCTTCCTCCCCCTCTCGCTCCCCATTTTCCACAATTCCAAGTCCCACCCCAAAATTTTCACTATTTCTCCAAAAGCTCCGGTCTTGATGATCCGAAATTGCACCTCCATCTCCGCCAAGCCTTCGTCGGAGCTCCGAAACAAGCGATCCAGTTCGGATTCTTCCGACGAGAAGCTACGCGCCCTCCGCCAGCTCTTCTCGAAGCCCGGCGTCGGCATCGACGCTTATATCATTCCTTCTCAGGATGCTCACCAGAGTGAGTTCATTGCTGAATGTTATATGAGGAGGACCTTTATATCGGGGTTCACAGGAAGTGCTGGAACTGCTGTCGTCACAAAGGATAAAGCTGCTCTTTGGACAGATGGGCGCTACTTTCTTCAGGCTGAAAAGCAGCTGAGCTCTAGTTGGATTCTCATGCGGGCAGGTTCTCTAGGAGTTCCTACGACTATTGAATGGTTGAATAATGTTTTGGCTCCTGGTGGGAGAGTTGGCATCGATCCTCAGTTTCTGTTTTCTTCCGAGGCTGCAGAAGAATTGAAACAAGGTATTGCAAAGAAGAACCATGAGTTGGTTTTCCTGTATGATAAAAATCTGGTGGACGAAGTATGGAATGAATCAAGACCAAAGCCTTCAAACAAACCAGTGAGAGTACATGATCTCAAGTATGCTGGTGTAGATGTAGCATCAAAGTTGTTGTCTTTGAGGTCGGAACTTGCTGATGCTGGTTCATCTGCAATAATTATATCTATGCTCGATGAAGTTGCCTGGCTGTTGAACTTGAGAGGAAGTGATGTTCCACATTCACCCGTTATGTATGCATATCTGATTGTAGAGATTGATAAAGCAAAATTATTTATAGATACTTCTAAAGTGAGCACAGAGGTGATGGATCACTTGAAGAAGGCAGGCATTGAAGTGTTGCCATATGACTCCATTCTTTCTGAAGTAGAAAGTCTGGCAGCAAAAGGGGCTAAAGTTTGGATGGATATATCTTCTCTTAATGCTGCTATTGTCAACACCTTTAACTCTGCTTGTGAAGGATATTATGAGAGCCTTAAAAGTAAAAGAAAAAGTAAGAGTGACGACTCAAATGGTTGGTCTGGAGGACCGACTGGAATTTATAGAGTGTCCCCTGTATCTTTGGCAAAGGCAGTTAAAAATGATTCTGAGTTGGAAGGCATGCAGAGTTGTCATTTAAGAGATGCCGCTGCTCTAGTGCAGTTTTGGGTTTGGATGGAGGAGAAAATTAACGAGAACATGAAATTGACTGAGGTAGAGGTTGCAGAAAAACTTCTTGAATTTCGTTCGGCGCAGTCTGGTTTCCTAGATACAAGCTTTGACACAATAAGCGGTTCTGGTGCAAATGGTGCTATCATACATTACAGAGCAGAACCAGATAGTTGTAGTGTTGTGGATGACAAGAAACTCTTCCTATTGGATAGCGGAGGTCAATATGTTGATGGAACAACTGACATAACTAGGACTGTACATTTTGGTGAACCTACCACACGACAAAAGGAATGCTTTACCCGAGTTTTGCAGGGTCATATAGCTCTTGATGAGGCAGTATTCCCTGAAAACATGCCCGGTTTTGTGTTAGATGCATTTGCTCGATCCTCCCTTTGGAAGATAGGACTGGATTATCGACATGGAACTGGGCATGGTGTGGGAGCTGCATTAAATGTTCATGAGGGGCCTCAAGGTATTAGCTTTCGATTTGGAAATATGACTCCGTTACAGAAGGGCATGATTGTTAGCAATGAACCTGGCTATTATGAAGATCATGCCTTTGGCATTCGGATTGAGAATCTCCTTGTTGTGAAAGAAGTCGACACACCAAATCGTTTTGGAGGAAATAGCTACCTGGGATTTGAAAAACTCACATTTGTCCCGATTCAGGCTAAAATGATCGACTTATCTTTGCTTTCCGCTGCGGAGTTTGATTGGCTTAATGATTACCATTCACAAGTTTGGGCGAAGGTCTCCCCTCTTGTGGAGGGTTCTGCTCGTCAATGGCTTTGGGACAACACAAGGCCGCTCGTGAAGCAGTGA

Protein Analysis

754

Amino Acids

83.72

Weight (kDa)

6.85

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 131 - 255 3.8e-12 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 277 - 460 9e-46 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 463 - 679 7.2e-50 Metallopeptidase family M24
Peptidase_M24_C PF16188 691 - 750 2.1e-24 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 778
AccBSI CCGCTC 2 cut(s) 195, 2248
AccI GTMKAC 1 cut(s) 2040
AccII CGCG 1 cut(s) 390
AclWI GGATC 5 cut(s) 295, 351, 701, 1107, 1818
AcsI RAATTY 3 cut(s) 264, 1326, 1508
AcuI CTGAAG 3 cut(s) 111, 572, 1173
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 2 cut(s) 861, 1698
AfiI CCNNNNNNNGG 5 cut(s) 422, 778, 1833, 2107, 2203
AjnI CCWGG 4 cut(s) 685, 974, 1975, 2073
AjuI GAANNNNNNNTTGG 4 cut(s) 662, 694, 761, 793
AleI CACNNNNGTG 2 cut(s) 465, 1091
AloI GAACNNNNNNTCC 2 cut(s) 987, 1019
Alw21I GWGCWC 3 cut(s) 345, 611, 1088
Alw26I GTCTC 2 cut(s) 15, 2197
AlwI GGATC 5 cut(s) 295, 351, 701, 1107, 1818
AlwNI CAGNNNCTG 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 1740
AoxI GGCC 2 cut(s) 1904, 2243
ApeKI GCWGC 8 cut(s) 557, 601, 734, 1169, 1214, 1418, 1883, 2141
ApoI RAATTY 3 cut(s) 264, 1326, 1508
ArsI GACNNNNNNTTYG 4 cut(s) 890, 922, 1292, 1324
Asp700I GAANNNNTTC 3 cut(s) 96, 1500, 1637
AspLEI GCGC 4 cut(s) 171, 392, 579, 1522
AspS9I GGNCC 3 cut(s) 498, 1316, 1904
AsuC2I CCSGG 2 cut(s) 417, 1797
AsuHPI GGTGA 5 cut(s) 85, 452, 1005, 1105, 1718
AvaI CYCGRG 1 cut(s) 1740
AvaII GGWCC 2 cut(s) 498, 1316
BanII GRGCYC 2 cut(s) 345, 611
BauI CACGAG 1 cut(s) 2249
Bbv12I GWGCWC 3 cut(s) 345, 611, 1088
BbvI GCAGC 8 cut(s) 544, 613, 721, 1181, 1201, 1405, 1870, 2128
BccI CCATC 6 cut(s) 326, 566, 1090, 1186, 1438, 1664
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 4 cut(s) 687, 976, 1977, 2075
BcnI CCSGG 2 cut(s) 417, 1797
BcoDI GTCTC 2 cut(s) 15, 2197
BfaI CTAG 5 cut(s) 612, 641, 1424, 1535, 1689
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 605
Bme1390I CCNGG 6 cut(s) 417, 687, 976, 1797, 1977, 2075
Bme18I GGWCC 2 cut(s) 498, 1316
BmeT110I CYCGRG 1 cut(s) 1740
BmgT120I GGNCC 3 cut(s) 498, 1316, 1904
BmiI GGNNCC 2 cut(s) 684, 1905
BmrFI CCNGG 6 cut(s) 417, 687, 976, 1797, 1977, 2075
BmrI ACTGGG 1 cut(s) 1875
BmsI GCATC 9 cut(s) 60, 132, 435, 445, 711, 902, 919, 1402, 1801
BmuI ACTGGG 1 cut(s) 1875
BpmI CTGGAG 1 cut(s) 1332
Bpu1102I GCTNAGC 1 cut(s) 605
BpuEI CTTGAG 3 cut(s) 854, 1009, 1893
BpuMI CCSGG 2 cut(s) 417, 1797
Bsa29I ATCGAT 1 cut(s) 705
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 2197
BsaJI CCNNGG 2 cut(s) 729, 2074
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 4 cut(s) 262, 292, 378, 408
Bsc4I CCNNNNNNNGG 5 cut(s) 422, 778, 1833, 2107, 2203
Bse1I ACTGG 5 cut(s) 360, 851, 1327, 1851, 1870
Bse3DI GCAATG 2 cut(s) 474, 1975
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 4 cut(s) 687, 976, 1977, 2075
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 729, 2074
BseGI GGATG 4 cut(s) 460, 1197, 1449, 1630
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 5 cut(s) 422, 778, 1833, 2107, 2203
BseMI GCAATG 2 cut(s) 474, 1975
BseMII CTCAG 6 cut(s) 119, 464, 596, 725, 1368, 1470
BseNI ACTGG 5 cut(s) 360, 851, 1327, 1851, 1870
BseRI GAGGAG 2 cut(s) 508, 1463
BseXI GCAGC 8 cut(s) 544, 613, 721, 1181, 1201, 1405, 1870, 2128
BsgI GTGCAG 1 cut(s) 1448
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 2 cut(s) 1906, 2245
BshVI ATCGAT 1 cut(s) 705
BsiHKAI GWGCWC 3 cut(s) 345, 611, 1088
BsiHKCI CYCGRG 1 cut(s) 1740
BsiSI CCGG 3 cut(s) 290, 417, 1797
BslFI GGGAC 4 cut(s) 238, 1324, 2084, 2246
BslI CCNNNNNNNGG 5 cut(s) 422, 778, 1833, 2107, 2203
BsmAI GTCTC 2 cut(s) 15, 2197
BsmFI GGGAC 4 cut(s) 238, 1324, 2084, 2246
BsmI GAATGC 2 cut(s) 1736, 2004
BsnI GGCC 2 cut(s) 1906, 2245
Bso31I GGTCTC 1 cut(s) 2197
BsoBI CYCGRG 1 cut(s) 1740
Bsp1286I GDGCHC 3 cut(s) 345, 611, 1088
Bsp1407I TGTACA 1 cut(s) 1696
Bsp143I GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
Bsp1720I GCTNAGC 1 cut(s) 605
BspANI GGCC 2 cut(s) 1906, 2245
BspCNI CTCAG 6 cut(s) 120, 463, 597, 724, 1369, 1471
BspDI ATCGAT 1 cut(s) 705
BspFNI CGCG 1 cut(s) 390
BspHI TCATGA 1 cut(s) 1897
BspLI GGNNCC 2 cut(s) 684, 1905
BspMAI CTGCAG 1 cut(s) 739
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 5 cut(s) 295, 351, 701, 1107, 1818
BspQI GCTCTTC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 2197
BsrBI CCGCTC 2 cut(s) 195, 2248
BsrDI GCAATG 2 cut(s) 474, 1975
BsrGI TGTACA 1 cut(s) 1696
BsrI ACTGG 5 cut(s) 360, 851, 1327, 1851, 1870
BssECI CCNNGG 2 cut(s) 729, 2074
BssMI GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
BssNI GRCGYC 1 cut(s) 420
BssSI CACGAG 1 cut(s) 2249
Bst2BI CACGAG 1 cut(s) 2249
Bst2UI CCWGG 4 cut(s) 687, 976, 1977, 2075
Bst4CI ACNGT 1 cut(s) 1696
Bst6I CTCTTC 2 cut(s) 410, 1643
BstACI GRCGYC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 1577
BstAUI TGTACA 1 cut(s) 1696
BstC8I GCNNGC 5 cut(s) 193, 401, 630, 1117, 1391
BstDEI CTNAG 6 cut(s) 128, 450, 605, 711, 1377, 1479
BstF5I GGATG 4 cut(s) 460, 1197, 1449, 1630
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 4 cut(s) 171, 392, 579, 1522
BstKTI GATC 8 cut(s) 303, 359, 709, 868, 1102, 1826, 1993, 2121
BstMAI GTCTC 2 cut(s) 15, 2197
BstMBI GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
BstMWI GCNNNNNNNGC 5 cut(s) 396, 533, 1178, 1577, 2254
BstNI CCWGG 4 cut(s) 687, 976, 1977, 2075
BstNSI RCATGY 2 cut(s) 1393, 1795
BstSCI CCNGG 6 cut(s) 415, 685, 974, 1795, 1975, 2073
BstSFI CTRYAG 1 cut(s) 735
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 8 cut(s) 544, 613, 721, 1181, 1201, 1405, 1870, 2128
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 2 cut(s) 1906, 2245
BsuTUI ATCGAT 1 cut(s) 705
BtsCI GGATG 4 cut(s) 460, 1197, 1449, 1630
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 858
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 5 cut(s) 193, 401, 630, 1117, 1391
CaiI CAGNNNCTG 1 cut(s) 718
CciI TCATGA 1 cut(s) 1897
CfoI GCGC 4 cut(s) 171, 392, 579, 1522
Cfr13I GGNCC 3 cut(s) 498, 1316, 1904
ClaI ATCGAT 1 cut(s) 705
CseI GACGC 2 cut(s) 409, 440
Csp6I GTAC 2 cut(s) 860, 1697
CviQI GTAC 2 cut(s) 860, 1697
DdeI CTNAG 6 cut(s) 128, 450, 605, 711, 1377, 1479
DpnI GATC 8 cut(s) 302, 358, 708, 867, 1101, 1825, 1992, 2120
DpnII GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
Eam1104I CTCTTC 2 cut(s) 410, 1643
EarI CTCTTC 2 cut(s) 410, 1643
EciI GGCGGA 4 cut(s) 178, 197, 313, 386
Ecl136II GAGCTC 2 cut(s) 343, 609
Eco24I GRGCYC 2 cut(s) 345, 611
Eco31I GGTCTC 1 cut(s) 2197
Eco47I GGWCC 2 cut(s) 498, 1316
Eco53kI GAGCTC 2 cut(s) 343, 609
Eco57I CTGAAG 3 cut(s) 111, 572, 1173
Eco88I CYCGRG 1 cut(s) 1740
EcoICRI GAGCTC 2 cut(s) 343, 609
EcoO109I RGGNCCY 2 cut(s) 498, 1904
EcoRII CCWGG 4 cut(s) 685, 974, 1975, 2073
EcoT22I ATGCAT 2 cut(s) 1030, 1816
EcoT38I GRGCYC 2 cut(s) 345, 611
FalI AAGNNNNNCTT 8 cut(s) 84, 116, 546, 578, 1622, 1654, 1718, 1750
FaqI GGGAC 4 cut(s) 238, 1324, 2084, 2246
FauI CCCGC 2 cut(s) 200, 621
FauNDI CATATG 1 cut(s) 1135
FblI GTMKAC 1 cut(s) 2040
FokI GGATG 4 cut(s) 467, 1204, 1456, 1637
FriOI GRGCYC 2 cut(s) 345, 611
FspBI CTAG 5 cut(s) 612, 641, 1424, 1535, 1689
GlaI GCGC 4 cut(s) 170, 391, 578, 1521
GsuI CTGGAG 1 cut(s) 1332
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 2 cut(s) 1906, 2245
HapII CCGG 3 cut(s) 290, 417, 1797
HgaI GACGC 2 cut(s) 409, 440
HhaI GCGC 4 cut(s) 171, 392, 579, 1522
Hin1I GRCGYC 1 cut(s) 420
Hin6I GCGC 4 cut(s) 169, 390, 577, 1520
HinP1I GCGC 4 cut(s) 169, 390, 577, 1520
HincII GTYRAC 2 cut(s) 1225, 2041
HindII GTYRAC 2 cut(s) 1225, 2041
HindIII AAGCTT 1 cut(s) 1543
HinfI GANTC 9 cut(s) 368, 619, 824, 1139, 1295, 1373, 1939, 2017, 2104
HpaII CCGG 3 cut(s) 290, 417, 1797
HphI GGTGA 5 cut(s) 85, 452, 1005, 1105, 1718
Hpy166II GTNNAC 5 cut(s) 516, 808, 1225, 1709, 2041
Hpy8I GTNNAC 5 cut(s) 516, 808, 1225, 1709, 2041
Hpy99I CGWCG 4 cut(s) 340, 380, 425, 434
HpyAV CCTTC 9 cut(s) 127, 342, 456, 849, 1105, 1241, 1379, 1945, 2182
HpyCH4III ACNGT 1 cut(s) 1696
HpyF10VI GCNNNNNNNGC 5 cut(s) 396, 533, 1178, 1577, 2254
HpyF3I CTNAG 6 cut(s) 128, 450, 605, 711, 1377, 1479
Hsp92I GRCGYC 1 cut(s) 420
HspAI GCGC 4 cut(s) 169, 390, 577, 1520
Kzo9I GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
LguI GCTCTTC 1 cut(s) 410
LmnI GCTCC 6 cut(s) 234, 292, 340, 348, 688, 1880
Lsp1109I GCAGC 8 cut(s) 544, 613, 721, 1181, 1201, 1405, 1870, 2128
LweI GCATC 9 cut(s) 60, 132, 435, 445, 711, 902, 919, 1402, 1801
MaeI CTAG 5 cut(s) 612, 641, 1424, 1535, 1689
MaeIII GTNAC 4 cut(s) 73, 541, 1289, 1944
MalI GATC 8 cut(s) 302, 358, 708, 867, 1101, 1825, 1992, 2120
MbiI CCGCTC 2 cut(s) 195, 2248
MboI GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
MhlI GDGCHC 3 cut(s) 345, 611, 1088
MlyI GAGTC 3 cut(s) 1133, 1289, 1933
MmeI TCCRAC 5 cut(s) 318, 399, 596, 897, 1362
Mph1103I ATGCAT 2 cut(s) 1030, 1816
MroXI GAANNNNTTC 3 cut(s) 96, 1500, 1637
MseI TTAA 9 cut(s) 45, 1209, 1233, 1266, 1365, 1406, 1458, 1889, 2160
MslI CAYNNNNRTG 4 cut(s) 465, 1091, 1124, 1704
MspA1I CMGCKG 3 cut(s) 604, 1418, 2141
MspI CCGG 3 cut(s) 290, 417, 1797
MspR9I CCNGG 6 cut(s) 417, 687, 976, 1797, 1977, 2075
Mva1269I GAATGC 2 cut(s) 1736, 2004
MvaI CCWGG 4 cut(s) 687, 976, 1977, 2075
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 5 cut(s) 396, 533, 1178, 1577, 2254
NciI CCSGG 2 cut(s) 417, 1797
NdeI CATATG 1 cut(s) 1135
NdeII GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
NlaIV GGNNCC 2 cut(s) 684, 1905
NmuCI GTSAC 3 cut(s) 73, 541, 1289
NsiI ATGCAT 2 cut(s) 1030, 1816
NspI RCATGY 2 cut(s) 1393, 1795
OliI CACNNNNGTG 2 cut(s) 465, 1091
PaeI GCATGC 1 cut(s) 1393
PagI TCATGA 1 cut(s) 1897
PciSI GCTCTTC 1 cut(s) 410
PctI GAATGC 2 cut(s) 1736, 2004
PdmI GAANNNNTTC 3 cut(s) 96, 1500, 1637
PfeI GAWTC 6 cut(s) 368, 619, 824, 1373, 2017, 2104
PflMI CCANNNNNTGG 1 cut(s) 778
PleI GAGTC 3 cut(s) 1133, 1289, 1933
PpsI GAGTC 3 cut(s) 1133, 1289, 1933
PpuMI RGGWCCY 1 cut(s) 498
Psp124BI GAGCTC 2 cut(s) 345, 611
Psp5II RGGWCCY 1 cut(s) 498
Psp6I CCWGG 4 cut(s) 685, 974, 1975, 2073
PspGI CCWGG 4 cut(s) 685, 974, 1975, 2073
PspN4I GGNNCC 2 cut(s) 684, 1905
PspPI GGNCC 3 cut(s) 498, 1316, 1904
PspPPI RGGWCCY 1 cut(s) 498
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 718
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 2 cut(s) 861, 1698
RsaNI GTAC 2 cut(s) 860, 1697
RseI CAYNNNNRTG 4 cut(s) 465, 1091, 1124, 1704
SacI GAGCTC 2 cut(s) 345, 611
SalI GTCGAC 1 cut(s) 2039
SapI GCTCTTC 1 cut(s) 410
SaqAI TTAA 9 cut(s) 45, 1209, 1233, 1266, 1365, 1406, 1458, 1889, 2160
Sau3AI GATC 8 cut(s) 300, 356, 706, 865, 1099, 1823, 1990, 2118
Sau96I GGNCC 3 cut(s) 498, 1316, 1904
SchI GAGTC 3 cut(s) 1133, 1289, 1933
ScrFI CCNGG 6 cut(s) 417, 687, 976, 1797, 1977, 2075
SduI GDGCHC 3 cut(s) 345, 611, 1088
SfaNI GCATC 9 cut(s) 60, 132, 435, 445, 711, 902, 919, 1402, 1801
SfcI CTRYAG 1 cut(s) 735
SinI GGWCC 2 cut(s) 498, 1316
SmiMI CAYNNNNRTG 4 cut(s) 465, 1091, 1124, 1704
SmlI CTYRAG 3 cut(s) 869, 988, 1908
SmoI CTYRAG 3 cut(s) 869, 988, 1908
SphI GCATGC 1 cut(s) 1393
SspMI CTAG 5 cut(s) 612, 641, 1424, 1535, 1689
SstI GAGCTC 2 cut(s) 345, 611
StyD4I CCNGG 6 cut(s) 415, 685, 974, 1795, 1975, 2073
TaaI ACNGT 1 cut(s) 1696
TaqI TCGA 9 cut(s) 410, 429, 705, 963, 1822, 1855, 1924, 2040, 2121
TaqII GACCGA 1 cut(s) 1333
TatI WGTACW 2 cut(s) 859, 1696
TauI GCSGC 3 cut(s) 213, 1418, 2248
TfiI GAWTC 6 cut(s) 368, 619, 824, 1373, 2017, 2104
Tru1I TTAA 9 cut(s) 45, 1209, 1233, 1266, 1365, 1406, 1458, 1889, 2160
Tru9I TTAA 9 cut(s) 45, 1209, 1233, 1266, 1365, 1406, 1458, 1889, 2160
TscAI CASTG 2 cut(s) 171, 858
TseFI GTSAC 3 cut(s) 73, 541, 1289
TseI GCWGC 8 cut(s) 557, 601, 734, 1169, 1214, 1418, 1883, 2141
Tsp45I GTSAC 3 cut(s) 73, 541, 1289
TspDTI ATGAA 8 cut(s) 463, 837, 927, 981, 1484, 1886, 1986, 2001
TspGWI ACGGA 1 cut(s) 1932
TspRI CASTG 2 cut(s) 171, 858
Van91I CCANNNNNTGG 1 cut(s) 778
VpaK11BI GGWCC 2 cut(s) 498, 1316
XapI RAATTY 3 cut(s) 264, 1326, 1508
XceI RCATGY 2 cut(s) 1393, 1795
XmiI GTMKAC 1 cut(s) 2040
XmnI GAANNNNTTC 3 cut(s) 96, 1500, 1637
XspI CTAG 5 cut(s) 612, 641, 1424, 1535, 1689
Zsp2I ATGCAT 2 cut(s) 1030, 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.