AT3G05480

Cell cycle checkpoint control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003050
Physical Location & Seq
Forward (+)
1585272 .. 1589292
4021 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05480.2

Sequence Viewer

Length: 1320 bp
ATGGAGTTGAGTATCAGTGGCAATGCGCTCAAGACCTTGTCTAGGTCTATAATCTGCCTCGCACGCGTCGGTAACGAGCTCGTTGTCCAAGCTTCGCCGACACAGCTGGCATTGCACACTTTGAATGCTTCACGTTCTGCCTATCAGTGTATCACATTTCAGTCAAGTTTCTTTGATGTATATACTGTTTCTGGTCCTCAAGCTCATTTCAGTGTACTTTTGAAGGCTGTTTGTTCTGTTCTTAGAACCCCTCTCGCGAGCATTGATCATATGAGTGTGCAGTTGCCAGATCATGATGCATCTAAGGTCAAATGGACTTTGCAGTGTTACAGTGGTATGAAGAAAACATATTGGATTACTTGCAATGTGGAACCAGATATACAGCATCTGTCCCTTGATAGGGGTAGATTTCCTAGTACCTTAGTCATGCATCCTCGAAATCTGAGCAAGTTGCTTGGAAACTTCCAGTCATCCCTCCAAGAGATTACAATTATTGCTACAGACCAAACTTCTTTCCCCTCTGATGCTGCAAGTGAAATAGGTGGAAAAGCTGTTGAGTTTCGGAGCTATGTGGATCCTACCAAAGATGGTGACTCTTTGTTACATACACAGCTATGGATTGATCCATCAGAAGAATTTTTGCAGTATACTCACGCAGGTGATCCAGTTGACATCACATTTTCCTTGAAGGAACTAAAGGCATTTCTTGCATTCTGCGAGGGCTGTGAAGCCGATATTCATCTTTTTTTCGAAAAAGCTGGCGAACCAATACTGATGGCTCCAAAATTCGGTTTGGGTGATGGGTCAAGCTCCAGTTTTGATGCAACACTCGTTCTTGCGACAATGCTTGTGTCTCAACTTCAGGAAGGGATTCCGGCAGAACCTCCTGAAGCAGCAAACTCCACAGGTGGCCATGCTGCCGAGCAAGTAGGATCACGGCCTCAAGAACGGTCTAGGCAAAATGCCTCTGAGCACCCATCTGATCACACACGAGTATGGTCTGAACTTTCAGGAACTGCCACTAAGAGCGTGAACGGTACTGAGGATAGGCCACAGGCTCAGGGACAACCAGACTTGGATATCCATAGAATTAGGAATATGGAAATATCAAAAGGCGGGCCAGTTGGAGATACTGCTCCTGCAGCTCCTCCTAAGTCCCAACGTCCCACACAGATAGATCATGCTGAAGGATCCCGAGTACGGGTACAGAACCAAAGTTTCTCCCAGCACCATCCTAGTAATTGGGTGGATGCAAATGAGGAAGAAGAAGATGACGACGAAGGTGTCGAGGCGACACCACCTCACAATGAAGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008408 GO:0009314 GO:0009628 GO:0009966 GO:0009967 GO:0009987 GO:0010212 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016787 GO:0016788 GO:0017124 GO:0019899 GO:0019900 GO:0019901 GO:0019904 GO:0022402 GO:0023051 GO:0023056 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0042826 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0045786 GO:0045930 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0104004 GO:1901360 GO:1902229 GO:1902231 GO:1902531 GO:1902533 GO:1903047 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Rad9

Protein Analysis

439

Amino Acids

48.02

Weight (kDa)

5.04

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad9 PF04139 13 - 282 3.3e-87 Rad9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 647
AasI GACNNNNNNGTC 1 cut(s) 1283
Acc36I ACCTGC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 647
AccII CGCG 2 cut(s) 66, 257
AciI CCGC 1 cut(s) 1116
AclWI GGATC 7 cut(s) 569, 582, 617, 656, 940, 1185, 1198
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 2 cut(s) 635, 785
AcuI CTGAAG 3 cut(s) 845, 909, 1206
AfaI GTAC 5 cut(s) 216, 418, 1039, 1200, 1206
AfiI CCNNNNNNNGG 6 cut(s) 42, 399, 400, 788, 1200, 1201
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 3 cut(s) 124, 223, 688
AleI CACNNNNGTG 1 cut(s) 657
Alw21I GWGCWC 2 cut(s) 81, 975
Alw26I GTCTC 1 cut(s) 858
AlwI GGATC 7 cut(s) 569, 582, 617, 656, 940, 1185, 1198
AlwNI CAGNNNCTG 2 cut(s) 388, 1016
Ama87I CYCGRG 1 cut(s) 1194
AoxI GGCC 4 cut(s) 910, 938, 1049, 1118
ApeKI GCWGC 4 cut(s) 527, 893, 917, 1142
ApoI RAATTY 2 cut(s) 635, 785
Asp700I GAANNNNTTC 1 cut(s) 870
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 2 cut(s) 194, 1118
AsuHPI GGTGA 3 cut(s) 602, 671, 809
AsuII TTCGAA 1 cut(s) 750
AvaI CYCGRG 1 cut(s) 1194
AvaII GGWCC 1 cut(s) 194
BalI TGGCCA 1 cut(s) 912
BamHI GGATCC 2 cut(s) 574, 1190
BanII GRGCYC 1 cut(s) 81
BauI CACGAG 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 81, 975
BbvI GCAGC 4 cut(s) 514, 904, 905, 1154
BccI CCATC 6 cut(s) 581, 634, 769, 794, 985, 1239
BceAI ACGGC 1 cut(s) 953
BclI TGATCA 2 cut(s) 265, 982
BcoDI GTCTC 1 cut(s) 858
BfaI CTAG 4 cut(s) 42, 414, 954, 1236
BfmI CTRYAG 2 cut(s) 498, 1140
BfuAI ACCTGC 1 cut(s) 647
BisI GCNGC 4 cut(s) 528, 894, 918, 1143
BlsI GCNGC 4 cut(s) 529, 895, 919, 1144
Bme18I GGWCC 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 1194
BmgT120I GGNCC 2 cut(s) 194, 1118
BmiI GGNNCC 4 cut(s) 372, 576, 780, 1192
BmsI GCATC 7 cut(s) 286, 308, 394, 439, 514, 811, 1240
BpmI CTGGAG 1 cut(s) 796
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 750
BpuEI CTTGAG 3 cut(s) 14, 183, 927
BsaBI GATNNNNATC 1 cut(s) 738
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 399, 400, 788, 1200, 1201
Bse1I ACTGG 4 cut(s) 466, 665, 813, 1121
Bse3DI GCAATG 3 cut(s) 28, 110, 370
Bse8I GATNNNNATC 1 cut(s) 738
BseGI GGATG 4 cut(s) 430, 470, 1231, 1255
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 6 cut(s) 42, 399, 400, 788, 1200, 1201
BseMI GCAATG 3 cut(s) 28, 110, 370
BseMII CTCAG 4 cut(s) 434, 960, 1032, 1073
BseNI ACTGG 4 cut(s) 466, 665, 813, 1121
BseRI GAGGAG 1 cut(s) 1137
BseXI GCAGC 4 cut(s) 514, 904, 905, 1154
BseYI CCCAGC 1 cut(s) 1224
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 2 cut(s) 66, 257
BshFI GGCC 4 cut(s) 912, 940, 1051, 1120
BsiHKAI GWGCWC 2 cut(s) 81, 975
BsiHKCI CYCGRG 1 cut(s) 1194
BsiSI CCGG 1 cut(s) 875
BslFI GGGAC 4 cut(s) 376, 1077, 1141, 1149
BslI CCNNNNNNNGG 6 cut(s) 42, 399, 400, 788, 1200, 1201
BsmAI GTCTC 1 cut(s) 858
BsmFI GGGAC 4 cut(s) 376, 1077, 1141, 1149
BsmI GAATGC 2 cut(s) 130, 710
BsnI GGCC 4 cut(s) 912, 940, 1051, 1120
BsoBI CYCGRG 1 cut(s) 1194
Bsp119I TTCGAA 1 cut(s) 750
Bsp1286I GDGCHC 2 cut(s) 81, 975
Bsp143I GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 1 cut(s) 1116
BspANI GGCC 4 cut(s) 912, 940, 1051, 1120
BspCNI CTCAG 4 cut(s) 435, 961, 1033, 1072
BspFNI CGCG 2 cut(s) 66, 257
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 4 cut(s) 372, 576, 780, 1192
BspMAI CTGCAG 1 cut(s) 1144
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 7 cut(s) 569, 582, 617, 656, 940, 1185, 1198
BspT104I TTCGAA 1 cut(s) 750
BsrDI GCAATG 3 cut(s) 28, 110, 370
BsrI ACTGG 4 cut(s) 466, 665, 813, 1121
BssMI GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
BssNAI GTATAC 1 cut(s) 648
BssSI CACGAG 1 cut(s) 990
Bst1107I GTATAC 1 cut(s) 648
Bst2BI CACGAG 1 cut(s) 990
Bst4CI ACNGT 4 cut(s) 187, 332, 951, 1037
BstAPI GCANNNNNTGC 1 cut(s) 707
BstBI TTCGAA 1 cut(s) 750
BstC8I GCNNGC 5 cut(s) 64, 108, 259, 760, 1118
BstDEI CTNAG 9 cut(s) 242, 303, 421, 443, 969, 1023, 1041, 1059, 1152
BstENI CCTNNNNNAGG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 430, 470, 1231, 1255
BstFNI CGCG 2 cut(s) 66, 257
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 9 cut(s) 268, 292, 577, 625, 664, 935, 985, 1180, 1193
BstMAI GTCTC 1 cut(s) 858
BstMBI GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
BstMWI GCNNNNNNNGC 5 cut(s) 63, 103, 112, 707, 1142
BstSFI CTRYAG 2 cut(s) 498, 1140
BstUI CGCG 2 cut(s) 66, 257
BstV1I GCAGC 4 cut(s) 514, 904, 905, 1154
BstX2I RGATCY 2 cut(s) 574, 1190
BstYI RGATCY 2 cut(s) 574, 1190
BstZ17I GTATAC 1 cut(s) 648
BsuRI GGCC 4 cut(s) 912, 940, 1051, 1120
BtsCI GGATG 4 cut(s) 430, 470, 1231, 1255
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 5 cut(s) 22, 152, 217, 329, 337
BtuMI TCGCGA 1 cut(s) 257
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 5 cut(s) 64, 108, 259, 760, 1118
CaiI CAGNNNCTG 2 cut(s) 388, 1016
CciI TCATGA 1 cut(s) 292
CfoI GCGC 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 194, 1118
CseI GACGC 1 cut(s) 55
Csp6I GTAC 5 cut(s) 215, 417, 1038, 1199, 1205
CviAII CATG 4 cut(s) 293, 427, 914, 1181
CviQI GTAC 5 cut(s) 215, 417, 1038, 1199, 1205
DdeI CTNAG 9 cut(s) 242, 303, 421, 443, 969, 1023, 1041, 1059, 1152
DpnI GATC 9 cut(s) 267, 291, 576, 624, 663, 934, 984, 1179, 1192
DpnII GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
DrdI GACNNNNNNGTC 1 cut(s) 1283
DseDI GACNNNNNNGTC 1 cut(s) 1283
EaeI YGGCCR 1 cut(s) 910
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 845, 909, 1206
Eco88I CYCGRG 1 cut(s) 1194
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 40
EcoRV GATATC 1 cut(s) 1081
EcoT22I ATGCAT 2 cut(s) 301, 432
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 4 cut(s) 296, 430, 917, 1184
FaqI GGGAC 4 cut(s) 376, 1077, 1141, 1149
FatI CATG 4 cut(s) 292, 426, 913, 1180
FauI CCCGC 1 cut(s) 1109
FauNDI CATATG 1 cut(s) 270
FbaI TGATCA 2 cut(s) 265, 982
FblI GTMKAC 1 cut(s) 647
Fnu4HI GCNGC 4 cut(s) 528, 894, 918, 1143
FokI GGATG 4 cut(s) 417, 457, 1218, 1262
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 528, 894, 918, 1143
FspBI CTAG 4 cut(s) 42, 414, 954, 1236
GlaI GCGC 1 cut(s) 27
GluI GCNGC 4 cut(s) 528, 894, 918, 1143
GsaI CCCAGC 1 cut(s) 1228
GsuI CTGGAG 1 cut(s) 796
HaeIII GGCC 4 cut(s) 912, 940, 1051, 1120
HapII CCGG 1 cut(s) 875
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 28
Hin1II CATG 4 cut(s) 296, 430, 917, 1184
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 593, 871
HpaII CCGG 1 cut(s) 875
HphI GGTGA 3 cut(s) 602, 671, 809
Hpy166II GTNNAC 4 cut(s) 215, 648, 670, 1033
Hpy188I TCNGA 7 cut(s) 444, 523, 564, 631, 970, 982, 1003
Hpy188III TCNNGA 8 cut(s) 31, 256, 293, 863, 887, 944, 1011, 1194
Hpy8I GTNNAC 4 cut(s) 215, 648, 670, 1033
Hpy99I CGWCG 2 cut(s) 71, 1280
HpyAV CCTTC 5 cut(s) 217, 682, 860, 1181, 1274
HpyCH4III ACNGT 4 cut(s) 187, 332, 951, 1037
HpyCH4IV ACGT 2 cut(s) 133, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 103, 112, 707, 1142
HpyF3I CTNAG 9 cut(s) 242, 303, 421, 443, 969, 1023, 1041, 1059, 1152
HpySE526I ACGT 2 cut(s) 133, 1162
Hsp92II CATG 4 cut(s) 296, 430, 917, 1184
HspAI GCGC 1 cut(s) 26
Ksp22I TGATCA 2 cut(s) 265, 982
Kzo9I GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
LmnI GCTCC 5 cut(s) 564, 784, 815, 1141, 1150
Lsp1109I GCAGC 4 cut(s) 514, 904, 905, 1154
LweI GCATC 7 cut(s) 286, 308, 394, 439, 514, 811, 1240
MaeI CTAG 4 cut(s) 42, 414, 954, 1236
MaeII ACGT 2 cut(s) 133, 1162
MaeIII GTNAC 4 cut(s) 71, 326, 590, 600
MalI GATC 9 cut(s) 267, 291, 576, 624, 663, 934, 984, 1179, 1192
MboI GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
MboII GAAGA 5 cut(s) 352, 644, 1274, 1277, 1280
MflI RGATCY 2 cut(s) 574, 1190
MhlI GDGCHC 2 cut(s) 81, 975
MlsI TGGCCA 1 cut(s) 912
MluCI AATT 5 cut(s) 489, 635, 785, 1089, 1240
MluI ACGCGT 1 cut(s) 64
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 1105
Mox20I TGGCCA 1 cut(s) 912
Mph1103I ATGCAT 2 cut(s) 301, 432
MroXI GAANNNNTTC 1 cut(s) 870
MscI TGGCCA 1 cut(s) 912
MseI TTAA 1 cut(s) 1318
MslI CAYNNNNRTG 5 cut(s) 210, 273, 613, 657, 994
Msp20I TGGCCA 1 cut(s) 912
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 1 cut(s) 875
Mva1269I GAATGC 2 cut(s) 130, 710
MvnI CGCG 2 cut(s) 66, 257
MwoI GCNNNNNNNGC 5 cut(s) 63, 103, 112, 707, 1142
NdeI CATATG 1 cut(s) 270
NdeII GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
NlaIII CATG 4 cut(s) 296, 430, 917, 1184
NlaIV GGNNCC 4 cut(s) 372, 576, 780, 1192
NmeAIII GCCGAG 1 cut(s) 946
NmuCI GTSAC 1 cut(s) 590
NruI TCGCGA 1 cut(s) 257
NsiI ATGCAT 2 cut(s) 301, 432
NspV TTCGAA 1 cut(s) 750
OliI CACNNNNGTG 1 cut(s) 657
PagI TCATGA 1 cut(s) 292
PaqCI CACCTGC 1 cut(s) 647
PcsI WCGNNNNNNNCGW 1 cut(s) 1284
PctI GAATGC 2 cut(s) 130, 710
PdmI GAANNNNTTC 1 cut(s) 870
PfeI GAWTC 1 cut(s) 871
PflFI GACNNNGTC 1 cut(s) 37
PkrI GCNGC 4 cut(s) 529, 895, 919, 1144
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 81
PspFI CCCAGC 1 cut(s) 1224
PspN4I GGNNCC 4 cut(s) 372, 576, 780, 1192
PspPI GGNCC 2 cut(s) 194, 1118
PsrI GAACNNNNNNTAC 2 cut(s) 363, 395
PstI CTGCAG 1 cut(s) 1144
PstNI CAGNNNCTG 2 cut(s) 388, 1016
PsuI RGATCY 2 cut(s) 574, 1190
PsyI GACNNNGTC 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 106
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 5 cut(s) 216, 418, 1039, 1200, 1206
RsaNI GTAC 5 cut(s) 215, 417, 1038, 1199, 1205
RseI CAYNNNNRTG 5 cut(s) 210, 273, 613, 657, 994
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 1318
SatI GCNGC 4 cut(s) 528, 894, 918, 1143
Sau3AI GATC 9 cut(s) 265, 289, 574, 622, 661, 932, 982, 1177, 1190
Sau96I GGNCC 2 cut(s) 194, 1118
SchI GAGTC 1 cut(s) 587
SduI GDGCHC 2 cut(s) 81, 975
SfaNI GCATC 7 cut(s) 286, 308, 394, 439, 514, 811, 1240
SfcI CTRYAG 2 cut(s) 498, 1140
SfuI TTCGAA 1 cut(s) 750
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 5 cut(s) 210, 273, 613, 657, 994
SmlI CTYRAG 3 cut(s) 29, 198, 942
SmoI CTYRAG 3 cut(s) 29, 198, 942
Sse9I AATT 5 cut(s) 489, 635, 785, 1089, 1240
SsiI CCGC 1 cut(s) 1116
SspMI CTAG 4 cut(s) 42, 414, 954, 1236
SstI GAGCTC 1 cut(s) 81
TaaI ACNGT 4 cut(s) 187, 332, 951, 1037
TaiI ACGT 2 cut(s) 136, 1165
TaqI TCGA 3 cut(s) 436, 750, 1287
TasI AATT 5 cut(s) 489, 635, 785, 1089, 1240
TatI WGTACW 1 cut(s) 214
TfiI GAWTC 1 cut(s) 871
Tru1I TTAA 1 cut(s) 1318
Tru9I TTAA 1 cut(s) 1318
TscAI CASTG 5 cut(s) 22, 152, 217, 329, 337
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 4 cut(s) 527, 893, 917, 1142
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 2 cut(s) 353, 728
TspRI CASTG 5 cut(s) 22, 152, 217, 329, 337
Tth111I GACNNNGTC 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 40
XapI RAATTY 2 cut(s) 635, 785
XmiI GTMKAC 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 870
XspI CTAG 4 cut(s) 42, 414, 954, 1236
Zsp2I ATGCAT 2 cut(s) 301, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.