Prupe.6G321300_v2.0.a1

Cell cycle checkpoint control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0003050
Physical Location & Seq
Reverse (-)
28399586 .. 28404389
4804 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G321300.1

Sequence Viewer

Length: 1335 bp
ATGGAATTCAGTGTGAGCGGGAACGCCCTGAAGACCTTCGCTCGCTCTATCACGTGCCTCGCACGCGTCGGAAACGAGCTCGCCATCCAAGCTTCGTCTTCTCAGCTTGCTTTGCATACTCTCAATTCGTCACGGTCTGCCTATCACTCAAGTACATTTAAGCCTGAATTCTTTGATGTCTATACTATATCTTGTAACCAGGTGCAATGTAGTGTGCTTTTGAAGGCGGTTTGTTCTGTGCTTAGGACACCTTTAGCAAGTATAGATCATTTGAGCATACGATTGCCTGATCCCGATGCTTCAAAAATGCAATGGACTTTAGAATGCTACAGTGGTATGAAGAAATCCTATTGGATTATTTGCAATGTTGAACCAGACATACAACATCTATCTCTTGATAGGAGAAGATTCCCAAGCAGCTTAGTTGTGAGACCTCGAGATCTAAACAGATTGCTTGCTAATTTTCAGTCATCGCTTCAGGAGATTACCATCATAGCAACAGAACCCACTTCCATGCCTTCTGATGTAGCAAGTGAAATTGGAGGAAAAGCTGTTGAACTTAGAAGTTATATTGACCCGACCAAAGACAGCGACTCATCACTGCATACTCAACTATGGATAGACCCTGCAGAAGAGTTTGTGCAGTATACCCACACGGGAGACCCCATAGATGTGACGTTTGGTGTGAAGGAGTTGAAGGCATTCCTTTCCTTCTGTGAGGGCTGTGAAGTTGACATTCACTTGTATTTTGAGAAAGCCGGAGACAGGCCTATTCTAATGGCACCCAAGTTCGGTCTAGATGATGGGTCCACTTCAAATTTTGATGCTACACTTGTACTTGCAACCATGCTAGTGTCGCAGCTCCATGAAAGCAATACCTCAGAACCTCCACAAGCAGCTACTGCAATGCACATTCAGGAAGATCATGGGACAGGGTCAGAACCACGACGAGAAAGGTGTGGAACAAATGTTTCGGAGCATCCATCTGATCACACCAGAATTTGGTCTGATCTTTCAGGAAGTGGTGCAAGAAGTGGCAGTGGTGCCGAAGCAGGACAGGCTCAAGGGGAAAGAAATTTGAGTGCTAATGGACAGAGAGAAATTCAAAGGATGAGCACAATGCATATTTCAAATCCTGCATGTGCTAGGGAAAATGAACCTGTGCAGGAGGATCATGTGGAAGAACCTCGAGATAGGTCACAAATTAACGGTGATGGCTTTTCACAACGTCATCCTAGCAACTGGATAGATGCAGATGAGGATGATAGTGATGAGGATGGAGAGGAGAATGAACTCTGTATTCCTTCAACACCACCATACTACGAAGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008408 GO:0009314 GO:0009628 GO:0009966 GO:0009967 GO:0009987 GO:0010212 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016787 GO:0016788 GO:0017124 GO:0019899 GO:0019900 GO:0019901 GO:0019904 GO:0022402 GO:0023051 GO:0023056 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0042826 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0045786 GO:0045930 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0104004 GO:1901360 GO:1902229 GO:1902231 GO:1902531 GO:1902533 GO:1903047 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Rad9

Protein Analysis

445

Amino Acids

49.2

Weight (kDa)

4.83

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 781, 1043
AccB7I CCANNNNNTGG 1 cut(s) 1002
AccBSI CCGCTC 1 cut(s) 18
AccI GTMKAC 1 cut(s) 647
AccII CGCG 1 cut(s) 66
AciI CCGC 2 cut(s) 18, 227
AclWI GGATC 2 cut(s) 284, 1179
AcsI RAATTY 6 cut(s) 5, 167, 817, 999, 1075, 1101
AcuI CTGAAG 2 cut(s) 50, 461
AcvI CACGTG 1 cut(s) 54
AfaI GTAC 2 cut(s) 154, 837
AfiI CCNNNNNNNGG 3 cut(s) 765, 791, 1002
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 9 cut(s) 223, 303, 371, 557, 697, 816, 1106, 1131, 1308
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 7 cut(s) 79, 92, 106, 420, 551, 862, 899
AluI AGCT 7 cut(s) 79, 92, 106, 420, 551, 862, 899
Alw21I GWGCWC 2 cut(s) 81, 1118
Alw26I GTCTC 3 cut(s) 424, 654, 756
AlwI GGATC 2 cut(s) 284, 1179
AlwNI CAGNNNCTG 1 cut(s) 902
Ama87I CYCGRG 2 cut(s) 435, 1188
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 3 cut(s) 417, 859, 896
ApoI RAATTY 6 cut(s) 5, 167, 817, 999, 1075, 1101
Asp700I GAANNNNTTC 2 cut(s) 35, 701
AspS9I GGNCC 1 cut(s) 807
AsuHPI GGTGA 1 cut(s) 1223
AvaI CYCGRG 2 cut(s) 435, 1188
AvaII GGWCC 1 cut(s) 807
BanI GGYRCC 2 cut(s) 781, 1043
BanII GRGCYC 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 38, 90
Bbv12I GWGCWC 2 cut(s) 81, 1118
BbvI GCAGC 3 cut(s) 429, 871, 908
BccI CCATC 6 cut(s) 92, 497, 797, 991, 1208, 1271
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 988
BcoDI GTCTC 3 cut(s) 424, 654, 756
BfaI CTAG 4 cut(s) 797, 851, 1146, 1236
BfmI CTRYAG 2 cut(s) 328, 627
BglII AGATCT 1 cut(s) 439
BisI GCNGC 3 cut(s) 418, 860, 897
BlsI GCNGC 3 cut(s) 419, 861, 898
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 807
BmeT110I CYCGRG 2 cut(s) 435, 1188
BmgT120I GGNCC 1 cut(s) 807
BmiI GGNNCC 3 cut(s) 783, 808, 1045
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 4 cut(s) 286, 814, 988, 1240
BpiI GAAGAC 2 cut(s) 38, 90
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 133, 1047
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 488
BsaI GGTCTC 2 cut(s) 424, 654
Bsc4I CCNNNNNNNGG 3 cut(s) 765, 791, 1002
Bse1I ACTGG 1 cut(s) 1247
Bse3DI GCAATG 4 cut(s) 212, 317, 370, 912
Bse8I GATNNNNATC 1 cut(s) 488
BseBI CCWGG 1 cut(s) 200
BseGI GGATG 6 cut(s) 84, 979, 1116, 1231, 1267, 1282
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 3 cut(s) 765, 791, 1002
BseMI GCAATG 4 cut(s) 212, 317, 370, 912
BseMII CTCAG 2 cut(s) 116, 894
BseNI ACTGG 1 cut(s) 1247
BseRI GAGGAG 1 cut(s) 1298
BseXI GCAGC 3 cut(s) 429, 871, 908
BsgI GTGCAG 2 cut(s) 662, 1184
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 769
BshNI GGYRCC 2 cut(s) 781, 1043
BsiHKAI GWGCWC 2 cut(s) 81, 1118
BsiHKCI CYCGRG 2 cut(s) 435, 1188
BsiSI CCGG 1 cut(s) 759
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 3 cut(s) 765, 791, 1002
BsmAI GTCTC 3 cut(s) 424, 654, 756
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 2 cut(s) 329, 701
BsnI GGCC 1 cut(s) 769
Bso31I GGTCTC 2 cut(s) 424, 654
BsoBI CYCGRG 2 cut(s) 435, 1188
Bsp1286I GDGCHC 2 cut(s) 81, 1118
Bsp143I GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
BspACI CCGC 2 cut(s) 18, 227
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 2 cut(s) 115, 893
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 783, 808, 1045
BspMAI CTGCAG 1 cut(s) 631
BspPI GGATC 2 cut(s) 284, 1179
BspT107I GGYRCC 2 cut(s) 781, 1043
BspTNI GGTCTC 2 cut(s) 424, 654
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 4 cut(s) 212, 317, 370, 912
BsrI ACTGG 1 cut(s) 1247
BssMI GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
BssNAI GTATAC 1 cut(s) 648
Bst1107I GTATAC 1 cut(s) 648
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 135, 332, 1211
Bst6I CTCTTC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 902
BstBAI YACGTR 1 cut(s) 54
BstC8I GCNNGC 5 cut(s) 43, 64, 81, 108, 456
BstDEI CTNAG 5 cut(s) 102, 242, 421, 560, 880
BstF5I GGATG 6 cut(s) 84, 979, 1116, 1231, 1267, 1282
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 7 cut(s) 268, 292, 442, 925, 991, 1012, 1174
BstMAI GTCTC 3 cut(s) 424, 654, 756
BstMBI GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
BstMWI GCNNNNNNNGC 6 cut(s) 63, 89, 112, 856, 902, 1058
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 1143
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 2 cut(s) 328, 627
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 429, 871, 908
BstV2I GAAGAC 2 cut(s) 38, 90
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BstZ17I GTATAC 1 cut(s) 648
BsuRI GGCC 1 cut(s) 769
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 6 cut(s) 84, 979, 1116, 1231, 1267, 1282
BtsI GCAGTG 2 cut(s) 599, 1045
BtsIMutI CAGTG 4 cut(s) 16, 337, 599, 1045
Cac8I GCNNGC 5 cut(s) 43, 64, 81, 108, 456
CaiI CAGNNNCTG 1 cut(s) 902
Cfr13I GGNCC 1 cut(s) 807
CseI GACGC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 198
Csp6I GTAC 2 cut(s) 153, 836
CviAII CATG 6 cut(s) 514, 847, 866, 926, 1140, 1175
CviQI GTAC 2 cut(s) 153, 836
DdeI CTNAG 5 cut(s) 102, 242, 421, 560, 880
DpnI GATC 7 cut(s) 267, 291, 441, 924, 990, 1011, 1173
DpnII GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 79
Eco147I AGGCCT 1 cut(s) 769
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 2 cut(s) 424, 654
Eco47I GGWCC 1 cut(s) 807
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 50, 461
Eco72I CACGTG 1 cut(s) 54
Eco88I CYCGRG 2 cut(s) 435, 1188
EcoICRI GAGCTC 1 cut(s) 79
EcoRI GAATTC 2 cut(s) 5, 167
EcoRII CCWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 1125
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 6 cut(s) 517, 850, 869, 929, 1143, 1178
FaqI GGGAC 1 cut(s) 943
FatI CATG 6 cut(s) 513, 846, 865, 925, 1139, 1174
FauI CCCGC 1 cut(s) 11
FbaI TGATCA 1 cut(s) 988
FblI GTMKAC 1 cut(s) 647
Fnu4HI GCNGC 3 cut(s) 418, 860, 897
FokI GGATG 6 cut(s) 71, 966, 1123, 1218, 1274, 1289
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 3 cut(s) 418, 860, 897
FspBI CTAG 4 cut(s) 797, 851, 1146, 1236
GluI GCNGC 3 cut(s) 418, 860, 897
HaeIII GGCC 1 cut(s) 769
HapII CCGG 1 cut(s) 759
HgaI GACGC 1 cut(s) 55
Hin1II CATG 6 cut(s) 517, 850, 869, 929, 1143, 1178
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 408, 593
HpaII CCGG 1 cut(s) 759
HphI GGTGA 1 cut(s) 1223
Hpy166II GTNNAC 3 cut(s) 648, 733, 810
Hpy188I TCNGA 7 cut(s) 71, 523, 883, 940, 976, 988, 1009
Hpy188III TCNNGA 8 cut(s) 293, 395, 437, 479, 797, 917, 1017, 1190
Hpy8I GTNNAC 3 cut(s) 648, 733, 810
Hpy99I CGWCG 2 cut(s) 71, 951
HpyAV CCTTC 7 cut(s) 46, 217, 528, 682, 691, 721, 1314
HpyCH4III ACNGT 3 cut(s) 135, 332, 1211
HpyCH4IV ACGT 3 cut(s) 53, 677, 1228
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 89, 112, 856, 902, 1058
HpyF3I CTNAG 5 cut(s) 102, 242, 421, 560, 880
HpySE526I ACGT 3 cut(s) 53, 677, 1228
Hsp92II CATG 6 cut(s) 517, 850, 869, 929, 1143, 1178
Ksp22I TGATCA 1 cut(s) 988
Kzo9I GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
LmnI GCTCC 2 cut(s) 867, 976
Lsp1109I GCAGC 3 cut(s) 429, 871, 908
LweI GCATC 4 cut(s) 286, 814, 988, 1240
MabI ACCWGGT 1 cut(s) 198
MaeI CTAG 4 cut(s) 797, 851, 1146, 1236
MaeII ACGT 3 cut(s) 53, 677, 1228
MaeIII GTNAC 4 cut(s) 129, 194, 673, 1197
MalI GATC 7 cut(s) 267, 291, 441, 924, 990, 1011, 1173
MbiI CCGCTC 1 cut(s) 18
MboI GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
MboII GAAGA 7 cut(s) 43, 90, 352, 417, 644, 932, 1193
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 81, 1118
MluI ACGCGT 1 cut(s) 64
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 49
Mph1103I ATGCAT 1 cut(s) 1125
MroXI GAANNNNTTC 2 cut(s) 35, 701
MseI TTAA 2 cut(s) 159, 1206
MslI CAYNNNNRTG 3 cut(s) 512, 671, 851
MspI CCGG 1 cut(s) 759
MspR9I CCNGG 1 cut(s) 200
Mva1269I GAATGC 2 cut(s) 329, 701
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 6 cut(s) 63, 89, 112, 856, 902, 1058
NdeII GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
NlaIII CATG 6 cut(s) 517, 850, 869, 929, 1143, 1178
NlaIV GGNNCC 3 cut(s) 783, 808, 1045
NmuCI GTSAC 3 cut(s) 129, 673, 1197
NsiI ATGCAT 1 cut(s) 1125
NspI RCATGY 1 cut(s) 1143
PaeR7I CTCGAG 2 cut(s) 435, 1188
PceI AGGCCT 1 cut(s) 769
PctI GAATGC 2 cut(s) 329, 701
PdmI GAANNNNTTC 2 cut(s) 35, 701
PfeI GAWTC 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 934
PflMI CCANNNNNTGG 1 cut(s) 1002
PkrI GCNGC 3 cut(s) 419, 861, 898
PleI GAGTC 1 cut(s) 587
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
PpsI GAGTC 1 cut(s) 587
Ppu21I YACGTR 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 198
PspCI CACGTG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 3 cut(s) 783, 808, 1045
PspPI GGNCC 1 cut(s) 807
PsrI GAACNNNNNNTAC 2 cut(s) 363, 395
PstI CTGCAG 1 cut(s) 631
PstNI CAGNNNCTG 1 cut(s) 902
PsuI RGATCY 1 cut(s) 439
PsyI GACNNNGTC 1 cut(s) 934
RsaI GTAC 2 cut(s) 154, 837
RsaNI GTAC 2 cut(s) 153, 836
RseI CAYNNNNRTG 3 cut(s) 512, 671, 851
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 159, 1206
SatI GCNGC 3 cut(s) 418, 860, 897
Sau3AI GATC 7 cut(s) 265, 289, 439, 922, 988, 1009, 1171
Sau96I GGNCC 1 cut(s) 807
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 81, 1118
SexAI ACCWGGT 1 cut(s) 198
SfaNI GCATC 4 cut(s) 286, 814, 988, 1240
SfcI CTRYAG 2 cut(s) 328, 627
Sfr274I CTCGAG 2 cut(s) 435, 1188
SinI GGWCC 1 cut(s) 807
SlaI CTCGAG 2 cut(s) 435, 1188
SmiMI CAYNNNNRTG 3 cut(s) 512, 671, 851
SmlI CTYRAG 4 cut(s) 148, 435, 1062, 1188
SmoI CTYRAG 4 cut(s) 148, 435, 1062, 1188
SseBI AGGCCT 1 cut(s) 769
SsiI CCGC 2 cut(s) 18, 227
SspMI CTAG 4 cut(s) 797, 851, 1146, 1236
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 769
StyD4I CCNGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 135, 332, 1211
TaiI ACGT 3 cut(s) 56, 680, 1231
TaqI TCGA 2 cut(s) 436, 1189
TaqII GACCGA 1 cut(s) 782
TatI WGTACW 2 cut(s) 152, 835
TfiI GAWTC 1 cut(s) 408
Tru1I TTAA 2 cut(s) 159, 1206
Tru9I TTAA 2 cut(s) 159, 1206
TscAI CASTG 4 cut(s) 16, 337, 606, 1045
TseFI GTSAC 3 cut(s) 129, 673, 1197
TseI GCWGC 3 cut(s) 417, 859, 896
Tsp45I GTSAC 3 cut(s) 129, 673, 1197
TspDTI ATGAA 4 cut(s) 353, 882, 1170, 1305
TspRI CASTG 4 cut(s) 16, 337, 606, 1045
Tth111I GACNNNGTC 1 cut(s) 934
Van91I CCANNNNNTGG 1 cut(s) 1002
VpaK11BI GGWCC 1 cut(s) 807
XapI RAATTY 6 cut(s) 5, 167, 817, 999, 1075, 1101
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 1 cut(s) 1143
XhoI CTCGAG 2 cut(s) 435, 1188
XmiI GTMKAC 1 cut(s) 647
XmnI GAANNNNTTC 2 cut(s) 35, 701
XspI CTAG 4 cut(s) 797, 851, 1146, 1236
Zsp2I ATGCAT 1 cut(s) 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.