MD04G1201200.v1.1

Cell cycle checkpoint control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003050
Physical Location & Seq
Reverse (-)
28738409 .. 28742041
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1201200.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGGAGTTCAGTGTGAGCGGCAACGCCCTGAAGACCTTCGCCCGCTCCATCACGTGCCTCGCACGCATCGGCAACGAGCTCGCCGTCCAAGCTTCTCCTACTCAGCTTGCTTTGCACTCCCTCAATACGTCACGTTCTGCCTATCAATCGAGCACATTTAAGCCTGATTTCTTTGACGTCTATACTGTAACTGGTAATCAGGTGCAATGTAGTGTGCTTTTAAAGGCTGTTTGCTCTGTGCTTAGGACACCTTTAGCAAGTATAGATAATTTGAGCGTGAAATTGCCTGATCCCGATGCATCAAAACTGCAATGGACTTTACAGTGCTTCAGTGGTATGAAAAAAACCTATTGGATTAGTTGCAATGTTGAACCCGATATACAACATTTGTCTCTTGATAGGAGAAAATTCCCAAGCGGCTTAGTGGTGAGACCCCGAGATCTGAACAGATTGCTGGCTAATTTTCAGTCATCGCTTCAGGAGATTACAGTCATAGCAACAGAACCCACTTCCTTGCCTTCTGATGCAGCAAGTGAAATTGGAGGAAAAGCTGTTGAACTTAGAAGTTATATTGACCCAACCAAAGACAGTGATTCATCACTGCATACTCAACTATGGATAGATCCTACTGAAGAGTTTGTGCAGTATACCCACACTGGAGACCCCATAGATGTGACATTCGGTGTGAAGGAGTTGAAGGCGTTCCTTTCTTTCTGCGAGGGCTGTGAAATTGACATCCACTTATATTTTGAGAAAGCTGGACAGCCTATTCTAATGGCACCAAAGTTTGGTCTAGATGATGGGTCCACTTCAAATTTTGATGCTACGCTTGTACTTGCAACCATGCTAGTGTCACAACTGCATGAAACCAATCCCGCAGAACCTCCACAAGCAGCTACCGAAATGCACATTCGGGATGAGCATGGGACAGGGTCAGAAGCACGACGAGAAAGGTGTAGGCCAAGTGGCTCTGAGCATCCATCTGACCACACCAGAATTTGGTCTGATCTTTCAGGAAGTGGTGCAAGAAGTGGCAACGGCACCGAAGGAGGACAGGTTCAAGGGGAAAGAAATTTGAATGCTAATGGACAGAGAGAAGTCCAAAGGATGAGTAAACTGCATATTTCGAATCCTGCATCTGCTGGGGGAAATGAGCCTGTGGATCCCAATGCCTGCCACCCTGAGGAAAATGATCATATGGAAGAACCTAGAGATAGATCACAAATTAACGGCGGTGGGTTTTCACAACACCATCCTAGTAACTGGGTAGACGCAGATGAAGACGACAGTGATGAGGATGAAGAGAATGACCTTTGTATTCAGGCAACACCACCATACTATGAAGAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008408 GO:0009314 GO:0009628 GO:0009966 GO:0009967 GO:0009987 GO:0010212 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016787 GO:0016788 GO:0017124 GO:0019899 GO:0019900 GO:0019901 GO:0019904 GO:0022402 GO:0023051 GO:0023056 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0042826 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0045786 GO:0045930 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0104004 GO:1901360 GO:1902229 GO:1902231 GO:1902531 GO:1902533 GO:1903047 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Rad9

Protein Analysis

451

Amino Acids

49.53

Weight (kDa)

4.82

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad9 PF04139 13 - 282 1.7e-77 Rad9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 2 cut(s) 778, 1040
AccB7I CCANNNNNTGG 2 cut(s) 788, 999
AccBSI CCGCTC 2 cut(s) 18, 45
AccI GTMKAC 2 cut(s) 647, 1269
AciI CCGC 5 cut(s) 18, 43, 417, 876, 1233
AclWI GGATC 4 cut(s) 284, 617, 1157, 1170
AcsI RAATTY 4 cut(s) 407, 814, 996, 1072
AcuI CTGAAG 4 cut(s) 50, 313, 461, 651
AcvI CACGTG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 834
AfiI CCNNNNNNNGG 3 cut(s) 788, 999, 1183
AgsI TTSAA 6 cut(s) 371, 557, 697, 813, 1061, 1078
AloI GAACNNNNNNTCC 2 cut(s) 1041, 1073
AluBI AGCT 6 cut(s) 79, 92, 106, 551, 758, 896
AluI AGCT 6 cut(s) 79, 92, 106, 551, 758, 896
Alw21I GWGCWC 2 cut(s) 81, 155
Alw26I GTCTC 3 cut(s) 396, 424, 654
AlwI GGATC 4 cut(s) 284, 617, 1157, 1170
Ama87I CYCGRG 1 cut(s) 435
AoxI GGCC 1 cut(s) 959
ApeKI GCWGC 2 cut(s) 527, 893
ApoI RAATTY 4 cut(s) 407, 814, 996, 1072
Asp700I GAANNNNTTC 2 cut(s) 35, 701
AspS9I GGNCC 1 cut(s) 804
AsuHPI GGTGA 1 cut(s) 439
AsuII TTCGAA 1 cut(s) 1127
AvaI CYCGRG 1 cut(s) 435
AvaII GGWCC 1 cut(s) 804
AxyI CCTNAGG 1 cut(s) 1182
BamHI GGATCC 1 cut(s) 1162
BanI GGYRCC 2 cut(s) 778, 1040
BanII GRGCYC 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 38, 1287
Bbv12I GWGCWC 2 cut(s) 81, 155
BbvI GCAGC 2 cut(s) 539, 905
BccI CCATC 4 cut(s) 56, 794, 988, 1260
BceAI ACGGC 3 cut(s) 68, 1054, 1246
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BclI TGATCA 1 cut(s) 1192
BcoDI GTCTC 3 cut(s) 396, 424, 654
BfaI CTAG 4 cut(s) 794, 848, 1209, 1257
BglII AGATCT 1 cut(s) 439
BisI GCNGC 4 cut(s) 19, 418, 528, 894
BlsI GCNGC 4 cut(s) 20, 419, 529, 895
Bme18I GGWCC 1 cut(s) 804
BmeT110I CYCGRG 1 cut(s) 435
BmgT120I GGNCC 1 cut(s) 804
BmiI GGNNCC 4 cut(s) 780, 805, 1042, 1164
BmrI ACTGGG 1 cut(s) 1273
BmsI GCATC 7 cut(s) 75, 286, 308, 514, 811, 985, 1145
BmuI ACTGGG 1 cut(s) 1273
BpiI GAAGAC 2 cut(s) 38, 1287
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 1127
BsaAI YACGTR 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 424, 654
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 999, 1183
Bse1I ACTGG 3 cut(s) 196, 661, 1268
Bse21I CCTNAGG 1 cut(s) 1182
Bse3DI GCAATG 3 cut(s) 212, 317, 370
BseGI GGATG 6 cut(s) 735, 922, 976, 1113, 1252, 1303
BseLI CCNNNNNNNGG 3 cut(s) 788, 999, 1183
BseMI GCAATG 3 cut(s) 212, 317, 370
BseMII CTCAG 3 cut(s) 116, 963, 1173
BseNI ACTGG 3 cut(s) 196, 661, 1268
BseXI GCAGC 2 cut(s) 539, 905
BseYI CCCAGC 1 cut(s) 1142
BsgI GTGCAG 1 cut(s) 662
BshFI GGCC 1 cut(s) 961
BshNI GGYRCC 2 cut(s) 778, 1040
BsiHKAI GWGCWC 2 cut(s) 81, 155
BsiHKCI CYCGRG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 940
BslI CCNNNNNNNGG 3 cut(s) 788, 999, 1183
BsmAI GTCTC 3 cut(s) 396, 424, 654
BsmFI GGGAC 1 cut(s) 940
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 1 cut(s) 961
Bso31I GGTCTC 2 cut(s) 424, 654
BsoBI CYCGRG 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 1127
Bsp1286I GDGCHC 2 cut(s) 81, 155
Bsp143I GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
BspACI CCGC 5 cut(s) 18, 43, 417, 876, 1233
BspANI GGCC 1 cut(s) 961
BspCNI CTCAG 3 cut(s) 115, 964, 1174
BspLI GGNNCC 4 cut(s) 780, 805, 1042, 1164
BspPI GGATC 4 cut(s) 284, 617, 1157, 1170
BspT104I TTCGAA 1 cut(s) 1127
BspT107I GGYRCC 2 cut(s) 778, 1040
BspTNI GGTCTC 2 cut(s) 424, 654
BsrBI CCGCTC 2 cut(s) 18, 45
BsrDI GCAATG 3 cut(s) 212, 317, 370
BsrI ACTGG 3 cut(s) 196, 661, 1268
BssMI GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
BssNAI GTATAC 1 cut(s) 648
BssNI GRCGYC 1 cut(s) 177
Bst1107I GTATAC 1 cut(s) 648
Bst4CI ACNGT 6 cut(s) 187, 324, 490, 590, 1289, 1350
Bst6I CTCTTC 2 cut(s) 627, 1296
BstACI GRCGYC 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 1127
BstC8I GCNNGC 6 cut(s) 43, 64, 81, 108, 456, 1174
BstDEI CTNAG 6 cut(s) 102, 242, 421, 560, 972, 1182
BstF5I GGATG 6 cut(s) 735, 922, 976, 1113, 1252, 1303
BstKTI GATC 7 cut(s) 292, 442, 625, 1009, 1165, 1195, 1220
BstMAI GTCTC 3 cut(s) 396, 424, 654
BstMBI GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
BstMWI GCNNNNNNNGC 3 cut(s) 63, 89, 112
BstV1I GCAGC 2 cut(s) 539, 905
BstV2I GAAGAC 2 cut(s) 38, 1287
BstX2I RGATCY 3 cut(s) 439, 622, 1162
BstYI RGATCY 3 cut(s) 439, 622, 1162
BstZ17I GTATAC 1 cut(s) 648
Bsu36I CCTNAGG 1 cut(s) 1182
BsuRI GGCC 1 cut(s) 961
BtgZI GCGATG 1 cut(s) 456
BtsCI GGATG 6 cut(s) 735, 922, 976, 1113, 1252, 1303
BtsI GCAGTG 1 cut(s) 599
BtsIMutI CAGTG 7 cut(s) 16, 329, 337, 595, 599, 654, 1294
Cac8I GCNNGC 6 cut(s) 43, 64, 81, 108, 456, 1174
Cfr13I GGNCC 1 cut(s) 804
CseI GACGC 1 cut(s) 1280
Csp6I GTAC 1 cut(s) 833
CviAII CATG 3 cut(s) 844, 863, 923
CviQI GTAC 1 cut(s) 833
DdeI CTNAG 6 cut(s) 102, 242, 421, 560, 972, 1182
DpnI GATC 7 cut(s) 291, 441, 624, 1008, 1164, 1194, 1219
DpnII GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
DraI TTTAAA 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 627, 1296
EarI CTCTTC 2 cut(s) 627, 1296
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 2 cut(s) 424, 654
Eco47I GGWCC 1 cut(s) 804
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 4 cut(s) 50, 313, 461, 651
Eco72I CACGTG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 1182
Eco88I CYCGRG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 79
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 1 cut(s) 81
FaeI CATG 3 cut(s) 847, 866, 926
FaqI GGGAC 1 cut(s) 940
FatI CATG 3 cut(s) 843, 862, 922
FauI CCCGC 2 cut(s) 50, 883
FauNDI CATATG 1 cut(s) 1197
FbaI TGATCA 1 cut(s) 1192
FblI GTMKAC 2 cut(s) 647, 1269
Fnu4HI GCNGC 4 cut(s) 19, 418, 528, 894
FokI GGATG 6 cut(s) 722, 929, 963, 1120, 1239, 1310
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 19, 418, 528, 894
FspBI CTAG 4 cut(s) 794, 848, 1209, 1257
GluI GCNGC 4 cut(s) 19, 418, 528, 894
GsaI CCCAGC 1 cut(s) 1146
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 1 cut(s) 961
HgaI GACGC 1 cut(s) 1280
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 847, 866, 926
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 593, 1129
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 4 cut(s) 648, 807, 1115, 1270
Hpy188I TCNGA 6 cut(s) 444, 523, 937, 973, 985, 1006
Hpy188III TCNNGA 6 cut(s) 293, 395, 479, 794, 914, 1014
Hpy8I GTNNAC 4 cut(s) 648, 807, 1115, 1270
Hpy99I CGWCG 1 cut(s) 948
HpyAV CCTTC 5 cut(s) 46, 528, 682, 691, 1040
HpyCH4III ACNGT 6 cut(s) 187, 324, 490, 590, 1289, 1350
HpyCH4IV ACGT 4 cut(s) 53, 128, 133, 177
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 89, 112
HpyF3I CTNAG 6 cut(s) 102, 242, 421, 560, 972, 1182
HpySE526I ACGT 4 cut(s) 53, 128, 133, 177
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 847, 866, 926
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 2 cut(s) 539, 905
LweI GCATC 7 cut(s) 75, 286, 308, 514, 811, 985, 1145
MaeI CTAG 4 cut(s) 794, 848, 1209, 1257
MaeII ACGT 4 cut(s) 53, 128, 133, 177
MaeIII GTNAC 5 cut(s) 129, 187, 673, 852, 1259
MalI GATC 7 cut(s) 291, 441, 624, 1008, 1164, 1194, 1219
MbiI CCGCTC 2 cut(s) 18, 45
MboI GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
MboII GAAGA 5 cut(s) 43, 644, 1214, 1292, 1313
MflI RGATCY 3 cut(s) 439, 622, 1162
MhlI GDGCHC 2 cut(s) 81, 155
MnlI CCTC 8 cut(s) 68, 131, 536, 712, 894, 1043, 1177, 1288
Mph1103I ATGCAT 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 35, 701
MseI TTAA 3 cut(s) 159, 221, 1227
MslI CAYNNNNRTG 2 cut(s) 671, 848
Mva1269I GAATGC 1 cut(s) 1084
MwoI GCNNNNNNNGC 3 cut(s) 63, 89, 112
NdeI CATATG 1 cut(s) 1197
NdeII GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
NlaIII CATG 3 cut(s) 847, 866, 926
NlaIV GGNNCC 4 cut(s) 780, 805, 1042, 1164
NmuCI GTSAC 3 cut(s) 129, 673, 852
NsiI ATGCAT 1 cut(s) 301
NspV TTCGAA 1 cut(s) 1127
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 1084
PdmI GAANNNNTTC 2 cut(s) 35, 701
PfeI GAWTC 2 cut(s) 593, 1129
PflFI GACNNNGTC 1 cut(s) 931
PflMI CCANNNNNTGG 2 cut(s) 788, 999
PkrI GCNGC 4 cut(s) 20, 419, 529, 895
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 81
PspCI CACGTG 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 1142
PspN4I GGNNCC 4 cut(s) 780, 805, 1042, 1164
PspPI GGNCC 1 cut(s) 804
PsrI GAACNNNNNNTAC 2 cut(s) 363, 395
PsuI RGATCY 3 cut(s) 439, 622, 1162
PsyI GACNNNGTC 1 cut(s) 931
RsaI GTAC 1 cut(s) 834
RsaNI GTAC 1 cut(s) 833
RseI CAYNNNNRTG 2 cut(s) 671, 848
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 159, 221, 1227
SatI GCNGC 4 cut(s) 19, 418, 528, 894
Sau3AI GATC 7 cut(s) 289, 439, 622, 1006, 1162, 1192, 1217
Sau96I GGNCC 1 cut(s) 804
SduI GDGCHC 2 cut(s) 81, 155
SfaNI GCATC 7 cut(s) 75, 286, 308, 514, 811, 985, 1145
SfuI TTCGAA 1 cut(s) 1127
SinI GGWCC 1 cut(s) 804
SmiMI CAYNNNNRTG 2 cut(s) 671, 848
SsiI CCGC 5 cut(s) 18, 43, 417, 876, 1233
SspMI CTAG 4 cut(s) 794, 848, 1209, 1257
SstI GAGCTC 1 cut(s) 81
TaaI ACNGT 6 cut(s) 187, 324, 490, 590, 1289, 1350
TaiI ACGT 4 cut(s) 56, 131, 136, 180
TaqI TCGA 2 cut(s) 149, 1127
TatI WGTACW 1 cut(s) 832
TauI GCSGC 2 cut(s) 21, 420
TfiI GAWTC 2 cut(s) 593, 1129
Tru1I TTAA 3 cut(s) 159, 221, 1227
Tru9I TTAA 3 cut(s) 159, 221, 1227
TscAI CASTG 7 cut(s) 16, 329, 337, 595, 606, 661, 1294
TseFI GTSAC 3 cut(s) 129, 673, 852
TseI GCWGC 2 cut(s) 527, 893
Tsp45I GTSAC 3 cut(s) 129, 673, 852
TspDTI ATGAA 5 cut(s) 353, 585, 879, 1293, 1314
TspRI CASTG 7 cut(s) 16, 329, 337, 595, 606, 661, 1294
Tth111I GACNNNGTC 1 cut(s) 931
Van91I CCANNNNNTGG 2 cut(s) 788, 999
VpaK11BI GGWCC 1 cut(s) 804
XapI RAATTY 4 cut(s) 407, 814, 996, 1072
XbaI TCTAGA 1 cut(s) 793
XmiI GTMKAC 2 cut(s) 647, 1269
XmnI GAANNNNTTC 2 cut(s) 35, 701
XspI CTAG 4 cut(s) 794, 848, 1209, 1257
ZraI GACGTC 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.