Rh3DG071000

Cell cycle checkpoint control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003050
Physical Location & Seq
Forward (+)
4810250 .. 4815498
5249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG071000.1

Sequence Viewer

Length: 1227 bp
ATGCAGCTGGCTTTTCACACTCTCAATGCATCAAGGTCTGCCTATCAATCGATTACATTTAAGCCTGACTTCTTTGATGTCTACAATATTGCTGTTGACCTTGTGCAATGTAGTGTGCTTTTGAAGGCTGTTTGTGCTGTTCTTAGGACGCCTATAGGAAGCATAGATCATTTGAGCGTGAATTTGCCTGATCCTGATGCATCAAAAGTGCAATGGACTTTAGAATGCTACAGTGGTATGAGGAAATCCTATTGGATTATTTGCAATGTTGAACCCGACGTACAACTTTTGGCACTTGATAGGACAAAATTCCCTAGCAGCCTAGTAGTGAGGCCTCGAGATCTCAACAGATTGCTTGCTAATTTTCAATCATCTCTTCAAGAGATCACTATTTTCGCAACAGAGCCTACTTCCTTACCTTCTGATGCAGCAAGTGAAATTGGAGGAAAAGCTGTTGAACTTAGAAGTTACATTGACCCAACCAAAGATAATGACTCATTACTGCATACTCAATTATGGATAGACCCTACAGAAGAGTTTGTACAGTATACCCACACGGGAGACCCTGTAGATGTGACATTTGCCATGAAGGAGTTGAAGGCATTTCTTTCGTTCTGTGAAGGCTGTGAAGTCGAAATTCACTTGTACTTTGAGAAGTCTGGAGAGCCTATTCTGATGGCACCGAGATTTGGTCTTGATGATACGTCCACTTCAAATTTTGATGCTACACTGGTGCTTGCAACCATGCTAGCGTCACAGCAAGGAGCTGCTGAAGTGCACATTCAGGATGAGAATGGGATGGGGTCAGAAGCACAGCATCAAAGATTCCGAACAAATGTCCCTGAGCATTCATCTGATCAGACAAGAATGTGGTCTGATCTTACAGGAAGTGGTGCGAAAAATGGTAGTGGTGCGGAAGTAGGACAGGTTCAAGGGGAAAGAGATTTGAATGCCAGTGCACAGAGAGATATTCAGAGGTTTAACACAATGCATATATCAAAGACTGCATCTGCTAGGGGAAATGAGCCTGTTGGTCTTAATATCTTCGACCCTGTTGAAAAGAATCATATGGAAGAGCCTCAAGATAGGTCAACAATGGTTGGTAATGGCTTATCACAACGTCATCCGAGCAACTGGGTAGGTGCAGATGAAGATGATAACGATGAGGATGGCGAGGAGGATGAATTGTGCATTCAGTCAACGCCACCATACTACGAAGAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000076 GO:0000077 GO:0000228 GO:0000278 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0008408 GO:0009314 GO:0009628 GO:0009966 GO:0009967 GO:0009987 GO:0010212 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0016787 GO:0016788 GO:0017124 GO:0019899 GO:0019900 GO:0019901 GO:0019904 GO:0022402 GO:0023051 GO:0023056 GO:0030896 GO:0031570 GO:0031573 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0042826 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0045786 GO:0045930 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071479 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0104004 GO:1901360 GO:1902229 GO:1902231 GO:1902531 GO:1902533 GO:1903047 GO:2001020 GO:2001022 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
Rad9

Protein Analysis

408

Amino Acids

45.4

Weight (kDa)

4.58

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad9 PF04139 2 - 249 3.2e-64 Rad9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 679
AccI GTMKAC 2 cut(s) 81, 548
AciI CCGC 1 cut(s) 914
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 4 cut(s) 181, 308, 636, 715
AcuI CTGAAG 1 cut(s) 792
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 3 cut(s) 282, 543, 647
AfiI CCNNNNNNNGG 1 cut(s) 689
AluBI AGCT 3 cut(s) 7, 452, 767
AluI AGCT 3 cut(s) 7, 452, 767
Alw21I GWGCWC 2 cut(s) 780, 961
Alw26I GTCTC 1 cut(s) 555
Alw44I GTGCAC 2 cut(s) 776, 957
AlwI GGATC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 1 cut(s) 332
ApaLI GTGCAC 2 cut(s) 776, 957
ApeKI GCWGC 4 cut(s) 4, 318, 428, 767
ApoI RAATTY 4 cut(s) 181, 308, 636, 715
AsuNHI GCTAGC 1 cut(s) 748
AvaI CYCGRG 1 cut(s) 336
BaeGI GKGCMC 2 cut(s) 780, 961
BanI GGYRCC 1 cut(s) 679
BarI GAAGNNNNNNTAC 2 cut(s) 525, 557
Bbv12I GWGCWC 2 cut(s) 780, 961
BbvI GCAGC 4 cut(s) 16, 330, 440, 754
BccI CCATC 3 cut(s) 670, 793, 1163
BcgI CGANNNNNNTGC 2 cut(s) 591, 625
BclI TGATCA 1 cut(s) 856
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 5 cut(s) 315, 323, 749, 1014, 1225
BfmI CTRYAG 4 cut(s) 153, 229, 528, 567
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 5, 319, 429, 768
BlsI GCNGC 4 cut(s) 6, 320, 430, 769
BmeT110I CYCGRG 1 cut(s) 336
BmiI GGNNCC 1 cut(s) 681
BmrI ACTGGG 1 cut(s) 1144
BmsI GCATC 7 cut(s) 38, 187, 209, 415, 712, 826, 1016
BmtI GCTAGC 1 cut(s) 752
BmuI ACTGGG 1 cut(s) 1144
BpmI CTGGAG 1 cut(s) 681
Bpu10I CCTNAGC 1 cut(s) 843
BpuEI CTTGAG 1 cut(s) 1065
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 555
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 1 cut(s) 689
Bse1I ACTGG 3 cut(s) 735, 954, 1139
Bse3DI GCAATG 3 cut(s) 113, 218, 271
BseCI ATCGAT 1 cut(s) 50
BseGI GGATG 5 cut(s) 793, 804, 1123, 1174, 1186
BseLI CCNNNNNNNGG 1 cut(s) 689
BseMI GCAATG 3 cut(s) 113, 218, 271
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 3 cut(s) 735, 954, 1139
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 2 cut(s) 780, 961
BseXI GCAGC 4 cut(s) 16, 330, 440, 754
BsgI GTGCAG 1 cut(s) 1164
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 780, 961
BsiHKCI CYCGRG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 1 cut(s) 689
BsmAI GTCTC 1 cut(s) 555
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 4 cut(s) 230, 847, 955, 1191
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 1 cut(s) 555
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 2 cut(s) 780, 961
Bsp1407I TGTACA 1 cut(s) 541
Bsp143I GATC 6 cut(s) 166, 190, 340, 384, 856, 877
BspACI CCGC 1 cut(s) 914
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 1 cut(s) 835
BspDI ATCGAT 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 681
BspOI GCTAGC 1 cut(s) 752
BspPI GGATC 1 cut(s) 185
BspQI GCTCTTC 1 cut(s) 1068
BspT107I GGYRCC 1 cut(s) 679
BspTNI GGTCTC 1 cut(s) 555
BsrDI GCAATG 3 cut(s) 113, 218, 271
BsrGI TGTACA 1 cut(s) 541
BsrI ACTGG 3 cut(s) 735, 954, 1139
BssMI GATC 6 cut(s) 166, 190, 340, 384, 856, 877
BssNAI GTATAC 1 cut(s) 549
BssNI GRCGYC 1 cut(s) 149
Bst1107I GTATAC 1 cut(s) 549
Bst4CI ACNGT 2 cut(s) 233, 546
Bst6I CTCTTC 3 cut(s) 381, 528, 1068
BstACI GRCGYC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 9, 357, 738, 750
BstDEI CTNAG 3 cut(s) 143, 461, 843
BstF5I GGATG 5 cut(s) 793, 804, 1123, 1174, 1186
BstKTI GATC 6 cut(s) 169, 193, 343, 387, 859, 880
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 6 cut(s) 166, 190, 340, 384, 856, 877
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstSFI CTRYAG 4 cut(s) 153, 229, 528, 567
BstSLI GKGCMC 2 cut(s) 780, 961
BstV1I GCAGC 4 cut(s) 16, 330, 440, 754
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BstZ17I GTATAC 1 cut(s) 549
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 1 cut(s) 334
BsuTUI ATCGAT 1 cut(s) 50
BtsCI GGATG 5 cut(s) 793, 804, 1123, 1174, 1186
BtsIMutI CAGTG 3 cut(s) 238, 728, 961
Cac8I GCNNGC 4 cut(s) 9, 357, 738, 750
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 2 cut(s) 157, 741
Csp6I GTAC 3 cut(s) 281, 542, 646
CviAII CATG 2 cut(s) 586, 745
CviQI GTAC 3 cut(s) 281, 542, 646
DdeI CTNAG 3 cut(s) 143, 461, 843
DpnI GATC 6 cut(s) 168, 192, 342, 386, 858, 879
DpnII GATC 6 cut(s) 166, 190, 340, 384, 856, 877
Eam1104I CTCTTC 3 cut(s) 381, 528, 1068
EarI CTCTTC 3 cut(s) 381, 528, 1068
Eco147I AGGCCT 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 555
Eco57I CTGAAG 1 cut(s) 792
Eco88I CYCGRG 1 cut(s) 336
EcoT22I ATGCAT 3 cut(s) 31, 202, 993
FaeI CATG 2 cut(s) 589, 748
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 824
FatI CATG 2 cut(s) 585, 744
FauNDI CATATG 1 cut(s) 1068
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 2 cut(s) 81, 548
Fnu4HI GCNGC 4 cut(s) 5, 319, 429, 768
FokI GGATG 5 cut(s) 800, 811, 1110, 1181, 1193
Fsp4HI GCNGC 4 cut(s) 5, 319, 429, 768
FspBI CTAG 5 cut(s) 315, 323, 749, 1014, 1225
GluI GCNGC 4 cut(s) 5, 319, 429, 768
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 1 cut(s) 334
HgaI GACGC 2 cut(s) 157, 741
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 2 cut(s) 589, 748
HincII GTYRAC 3 cut(s) 97, 1092, 1200
HindII GTYRAC 3 cut(s) 97, 1092, 1200
HinfI GANTC 3 cut(s) 494, 825, 1063
Hpy166II GTNNAC 8 cut(s) 82, 97, 549, 708, 778, 959, 1092, 1200
Hpy188I TCNGA 9 cut(s) 424, 675, 808, 830, 856, 861, 877, 975, 1128
Hpy188III TCNNGA 7 cut(s) 194, 338, 380, 660, 695, 785, 1082
Hpy8I GTNNAC 8 cut(s) 82, 97, 549, 708, 778, 959, 1092, 1200
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 5 cut(s) 118, 429, 583, 592, 614
HpyCH4III ACNGT 2 cut(s) 233, 546
HpyCH4IV ACGT 3 cut(s) 279, 704, 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 143, 461, 843
HpySE526I ACGT 3 cut(s) 279, 704, 1120
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 2 cut(s) 589, 748
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 6 cut(s) 166, 190, 340, 384, 856, 877
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 1 cut(s) 764
Lsp1109I GCAGC 4 cut(s) 16, 330, 440, 754
LweI GCATC 7 cut(s) 38, 187, 209, 415, 712, 826, 1016
MaeI CTAG 5 cut(s) 315, 323, 749, 1014, 1225
MaeII ACGT 3 cut(s) 279, 704, 1120
MaeIII GTNAC 3 cut(s) 467, 574, 753
MalI GATC 6 cut(s) 168, 192, 342, 386, 858, 879
MboI GATC 6 cut(s) 166, 190, 340, 384, 856, 877
MboII GAAGA 5 cut(s) 368, 545, 1036, 1085, 1163
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 780, 961
MluCI AATT 8 cut(s) 181, 308, 361, 438, 512, 636, 715, 1184
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 9 cut(s) 234, 324, 345, 437, 969, 1089, 1159, 1168, 1171
Mph1103I ATGCAT 3 cut(s) 31, 202, 993
MseI TTAA 3 cut(s) 60, 981, 1038
MspA1I CMGCKG 1 cut(s) 7
Mva1269I GAATGC 4 cut(s) 230, 847, 955, 1191
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeI CATATG 1 cut(s) 1068
NdeII GATC 6 cut(s) 166, 190, 340, 384, 856, 877
NheI GCTAGC 1 cut(s) 748
NlaIII CATG 2 cut(s) 589, 748
NlaIV GGNNCC 1 cut(s) 681
NmuCI GTSAC 2 cut(s) 574, 753
NsiI ATGCAT 3 cut(s) 31, 202, 993
PaeR7I CTCGAG 1 cut(s) 336
PceI AGGCCT 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 1068
PctI GAATGC 4 cut(s) 230, 847, 955, 1191
PfeI GAWTC 2 cut(s) 825, 1063
PkrI GCNGC 4 cut(s) 6, 320, 430, 769
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 681
PsrI GAACNNNNNNTAC 4 cut(s) 264, 296, 912, 944
PsuI RGATCY 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 7
RsaI GTAC 3 cut(s) 282, 543, 647
RsaNI GTAC 3 cut(s) 281, 542, 646
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 3 cut(s) 60, 981, 1038
SatI GCNGC 4 cut(s) 5, 319, 429, 768
Sau3AI GATC 6 cut(s) 166, 190, 340, 384, 856, 877
SchI GAGTC 1 cut(s) 488
SduI GDGCHC 2 cut(s) 780, 961
SfaNI GCATC 7 cut(s) 38, 187, 209, 415, 712, 826, 1016
SfcI CTRYAG 4 cut(s) 153, 229, 528, 567
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 336, 1080
SmoI CTYRAG 2 cut(s) 336, 1080
Sse9I AATT 8 cut(s) 181, 308, 361, 438, 512, 636, 715, 1184
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 1 cut(s) 914
SspI AATATT 1 cut(s) 88
SspMI CTAG 5 cut(s) 315, 323, 749, 1014, 1225
StuI AGGCCT 1 cut(s) 334
TaaI ACNGT 2 cut(s) 233, 546
TaiI ACGT 3 cut(s) 282, 707, 1123
TaqI TCGA 4 cut(s) 50, 337, 633, 1047
TasI AATT 8 cut(s) 181, 308, 361, 438, 512, 636, 715, 1184
TatI WGTACW 2 cut(s) 541, 645
TfiI GAWTC 2 cut(s) 825, 1063
Tru1I TTAA 3 cut(s) 60, 981, 1038
Tru9I TTAA 3 cut(s) 60, 981, 1038
TscAI CASTG 3 cut(s) 238, 735, 961
TseFI GTSAC 2 cut(s) 574, 753
TseI GCWGC 4 cut(s) 4, 318, 428, 767
Tsp45I GTSAC 2 cut(s) 574, 753
TspDTI ATGAA 4 cut(s) 602, 840, 1164, 1197
TspRI CASTG 3 cut(s) 238, 735, 961
VneI GTGCAC 2 cut(s) 776, 957
XapI RAATTY 4 cut(s) 181, 308, 636, 715
XhoI CTCGAG 1 cut(s) 336
XmiI GTMKAC 2 cut(s) 81, 548
XspI CTAG 5 cut(s) 315, 323, 749, 1014, 1225
Zsp2I ATGCAT 3 cut(s) 31, 202, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.