AT3G10720

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011631
Physical Location & Seq
Reverse (-)
3354406 .. 3357754
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10720.1

Sequence Viewer

Length: 792 bp
ATGCTTATGGGAGATGGGATCAATAAGACGATTATTACAGGGAACCATAATGTTGTTGATGGTTGGACTACGTATAATTGCTCGAGCTTCGCTGTGGTTGGAGAGCGGTTCATGGCGGTTGATGTTACATTCAGAAACACAGCCGGACCTGAAAAACATCAGGCAGTGGCTTTGAGGAACAATGCGGAAGGATCAAGTTTTTATCGGTGTAGTTTTGAAGGCTATCAAGATACTCTCTACGTCCATTCTCTTAGACAATTCTATCGCGAATGCGATATATATGGTACCGTCGATTTTATATTCGGAAATGCAGCCGCAATTTTTCAAAACTGCAACATATATGCTCGAAAACCGATGGCCAAGCAAAAGAACGCAATAACAGCGCATGGAAGACTCGATCCAAACCAGAATACCGGAATCTCTATCATCAATTGTACAATAAAAGCCGCCCCAGATCTTGCAGCTGAGCCTAAATCTGCCATGACATTCTTAGGGAGACCGTGGAAGCCATACTCAAGGACGGTTTTCATGCAATCATACATTAGTGACATTGTTCAACCAGTCGGATGGCTCGAATGGAACGGTACAATTGGACTAGACACAATCTACTACGGTGAGTATAGTAACTTTGGACCAGGTGCTAACACGAACCAGAGAGTGCAGTGGTTAGGGTACAATTTATTGAACTTGGCAGAAGCCATGAACTTCACGGTCTACAACTTCACCATGGGAGATACATGGTTGCCTCAAACCGATATTCCTTTCTATGGTGGTTTGCTTAGCAAAGAATGA

Protein Analysis

263

Amino Acids

29.52

Weight (kDa)

6.09

Isoelectric Point (pI)

21.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 1 - 245 1.2e-120 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 284
AccBSI CCGCTC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 714
AccII CGCG 1 cut(s) 267
AciI CCGC 5 cut(s) 106, 116, 185, 315, 447
AclWI GGATC 3 cut(s) 26, 199, 392
AcoI YGGCCR 1 cut(s) 357
AfaI GTAC 4 cut(s) 286, 436, 586, 674
AfiI CCNNNNNNNGG 1 cut(s) 767
AgsI TTSAA 4 cut(s) 218, 326, 557, 685
AjnI CCWGG 1 cut(s) 634
AluBI AGCT 2 cut(s) 87, 464
AluI AGCT 2 cut(s) 87, 464
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 3 cut(s) 26, 199, 392
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 2 cut(s) 311, 461
Asp718I GGTACC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 146, 632
AsuHPI GGTGA 2 cut(s) 626, 715
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 2 cut(s) 146, 632
BalI TGGCCA 1 cut(s) 359
BanI GGYRCC 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 397
BbvI GCAGC 2 cut(s) 323, 473
BccI CCATC 4 cut(s) 8, 53, 349, 561
BciT130I CCWGG 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 1 cut(s) 596
BglII AGATCT 1 cut(s) 454
BisI GCNGC 4 cut(s) 312, 315, 447, 462
BlpI GCTNAGC 2 cut(s) 465, 779
BlsI GCNGC 4 cut(s) 313, 316, 448, 463
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 2 cut(s) 146, 632
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 146, 632
BmiI GGNNCC 2 cut(s) 44, 286
BmrFI CCNGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 397
Bpu1102I GCTNAGC 2 cut(s) 465, 779
BpuEI CTTGAG 1 cut(s) 499
BsaAI YACGTR 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 500, 726
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 1 cut(s) 560
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 500, 726
BseGI GGATG 1 cut(s) 572
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 560
BseXI GCAGC 2 cut(s) 323, 473
BsgI GTGCAG 1 cut(s) 680
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 359
BshNI GGYRCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 2 cut(s) 144, 414
BslI CCNNNNNNNGG 1 cut(s) 767
BsmAI GTCTC 1 cut(s) 490
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 359
Bso31I GGTCTC 1 cut(s) 490
BsoBI CYCGRG 1 cut(s) 82
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 4 cut(s) 18, 191, 397, 454
Bsp1720I GCTNAGC 2 cut(s) 465, 779
Bsp19I CCATGG 1 cut(s) 726
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 5 cut(s) 106, 116, 185, 315, 447
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 1 cut(s) 457
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 44, 286
BspPI GGATC 3 cut(s) 26, 199, 392
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 500, 726
BssMI GATC 4 cut(s) 18, 191, 397, 454
BssT1I CCWWGG 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 6 cut(s) 289, 501, 523, 584, 614, 712
BstAUI TGTACA 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 72
BstDEI CTNAG 4 cut(s) 251, 465, 490, 779
BstDSI CCRYGG 2 cut(s) 500, 726
BstF5I GGATG 1 cut(s) 572
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 4 cut(s) 21, 194, 400, 457
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 4 cut(s) 18, 191, 397, 454
BstMWI GCNNNNNNNGC 1 cut(s) 380
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 634
BstSNI TACGTA 1 cut(s) 72
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 2 cut(s) 323, 473
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 567
BstYI RGATCY 1 cut(s) 454
BsuRI GGCC 1 cut(s) 359
BtgI CCRYGG 2 cut(s) 500, 726
BtsCI GGATG 1 cut(s) 572
BtsI GCAGTG 2 cut(s) 171, 668
BtsIMutI CAGTG 2 cut(s) 171, 668
BtuMI TCGCGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 146, 632
CsiI ACCWGGT 1 cut(s) 634
Csp6I GTAC 4 cut(s) 285, 435, 585, 673
CviAII CATG 7 cut(s) 112, 386, 481, 529, 700, 727, 738
CviQI GTAC 4 cut(s) 285, 435, 585, 673
DdeI CTNAG 4 cut(s) 251, 465, 490, 779
DpnI GATC 4 cut(s) 20, 193, 399, 456
DpnII GATC 4 cut(s) 18, 191, 397, 454
EaeI YGGCCR 1 cut(s) 357
Eco105I TACGTA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 726
Eco31I GGTCTC 1 cut(s) 490
Eco47I GGWCC 2 cut(s) 146, 632
Eco88I CYCGRG 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 726
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 7 cut(s) 115, 389, 484, 532, 703, 730, 741
FatI CATG 7 cut(s) 111, 385, 480, 528, 699, 726, 737
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 4 cut(s) 312, 315, 447, 462
FokI GGATG 1 cut(s) 579
Fsp4HI GCNGC 4 cut(s) 312, 315, 447, 462
FspBI CTAG 1 cut(s) 596
GlaI GCGC 1 cut(s) 384
GluI GCNGC 4 cut(s) 312, 315, 447, 462
HaeIII GGCC 1 cut(s) 359
HapII CCGG 2 cut(s) 144, 414
HhaI GCGC 1 cut(s) 385
Hin1II CATG 7 cut(s) 115, 389, 484, 532, 703, 730, 741
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 2 cut(s) 393, 417
HpaII CCGG 2 cut(s) 144, 414
HphI GGTGA 2 cut(s) 626, 715
Hpy166II GTNNAC 1 cut(s) 715
Hpy188I TCNGA 3 cut(s) 134, 305, 566
Hpy188III TCNNGA 2 cut(s) 227, 266
Hpy8I GTNNAC 1 cut(s) 715
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 182, 212
HpyCH4III ACNGT 6 cut(s) 289, 501, 523, 584, 614, 712
HpyCH4IV ACGT 2 cut(s) 71, 240
HpyCH4V TGCA 5 cut(s) 311, 333, 461, 532, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 380
HpyF3I CTNAG 4 cut(s) 251, 465, 490, 779
HpySE526I ACGT 2 cut(s) 71, 240
Hsp92II CATG 7 cut(s) 115, 389, 484, 532, 703, 730, 741
HspAI GCGC 1 cut(s) 383
KpnI GGTACC 1 cut(s) 288
Kzo9I GATC 4 cut(s) 18, 191, 397, 454
Lsp1109I GCAGC 2 cut(s) 323, 473
MabI ACCWGGT 1 cut(s) 634
MaeI CTAG 1 cut(s) 596
MaeII ACGT 2 cut(s) 71, 240
MaeIII GTNAC 3 cut(s) 124, 545, 623
MalI GATC 4 cut(s) 20, 193, 399, 456
MbiI CCGCTC 1 cut(s) 106
MboI GATC 4 cut(s) 18, 191, 397, 454
MboII GAAGA 1 cut(s) 402
MfeI CAATTG 2 cut(s) 430, 588
MflI RGATCY 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 6 cut(s) 76, 257, 318, 430, 588, 676
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 1 cut(s) 387
MmeI TCCRAC 3 cut(s) 44, 79, 544
MnlI CCTC 2 cut(s) 168, 756
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 144, 414
MspR9I CCNGG 1 cut(s) 636
MunI CAATTG 2 cut(s) 430, 588
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 380
NcoI CCATGG 1 cut(s) 726
NdeII GATC 4 cut(s) 18, 191, 397, 454
NlaIII CATG 7 cut(s) 115, 389, 484, 532, 703, 730, 741
NlaIV GGNNCC 2 cut(s) 44, 286
NmuCI GTSAC 1 cut(s) 545
NruI TCGCGA 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 1 cut(s) 417
PkrI GCNGC 4 cut(s) 313, 316, 448, 463
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
Ppu21I YACGTR 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 2 cut(s) 44, 286
PspPI GGNCC 2 cut(s) 146, 632
PspXI VCTCGAGB 1 cut(s) 82
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 464
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 4 cut(s) 286, 436, 586, 674
RsaNI GTAC 4 cut(s) 285, 435, 585, 673
SatI GCNGC 4 cut(s) 312, 315, 447, 462
Sau3AI GATC 4 cut(s) 18, 191, 397, 454
Sau96I GGNCC 2 cut(s) 146, 632
SchI GAGTC 1 cut(s) 387
ScrFI CCNGG 1 cut(s) 636
SetI ASST 6 cut(s) 74, 89, 151, 243, 466, 640
SexAI ACCWGGT 1 cut(s) 634
Sfr274I CTCGAG 1 cut(s) 82
SinI GGWCC 2 cut(s) 146, 632
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 2 cut(s) 82, 514
SmoI CTYRAG 2 cut(s) 82, 514
SnaBI TACGTA 1 cut(s) 72
Sse9I AATT 6 cut(s) 76, 257, 318, 430, 588, 676
SsiI CCGC 5 cut(s) 106, 116, 185, 315, 447
SspMI CTAG 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 634
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 6 cut(s) 289, 501, 523, 584, 614, 712
TaiI ACGT 2 cut(s) 74, 243
TaqI TCGA 5 cut(s) 83, 291, 346, 396, 573
TasI AATT 6 cut(s) 76, 257, 318, 430, 588, 676
TatI WGTACW 1 cut(s) 434
TauI GCSGC 2 cut(s) 317, 449
TfiI GAWTC 1 cut(s) 417
TscAI CASTG 2 cut(s) 171, 668
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 2 cut(s) 311, 461
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 3 cut(s) 100, 517, 716
TspRI CASTG 2 cut(s) 171, 668
VpaK11BI GGWCC 2 cut(s) 146, 632
XhoI CTCGAG 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 714
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.