Rroxscaffold_4G00319570

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011631
Physical Location & Seq
Reverse (-)
47566173 .. 47571128
4956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319570.1

Sequence Viewer

Length: 1263 bp
ATGACCGACGCCGAGGCCAACGCTTTCAAGGACTGCCAGCAGTTGTCGGAGCTGAACGTCGACTACTTGGAGGCCATCTCGACGGAGCTCAAGTCGGCCGAGCTGCTCAACCAGGAGCTGGTGGACAAGGTCCAGACTCTGCTGAGTGGGATAGTGACAAACCAGCAGACCTGCTACGATGGGCTGGTTGTGGCCAAGAGCAGCATTGTCGACACTAGCACGAGTCCCTTGTCCGTAGCCTTGTCCAATGCCACGCAGCTCTACAGTGTATCGCTTGGGTTGGTCACTCATTCTTTGGCTCGAAACCTCAAGAAGCATAAGAAACGAAACGGCTTTGTTTCTCAGGGTCACAAAATCCGGGAGCCACTTGAAACATTCATCGAGGCTCTAAGGAAGAGTTCTTCAAAAAATTCGAGGGGTGAGAGAATTCTCGAGGAAATGGAGAGCAGTGGCATTATAGTTAATGACACTGTCATTGTGAGTCCACATGGTACGGACAACTTCACTTCCATTGGAGCTGCCATTGAGTCTGCACCTAACAACACAGAGCCAGAAGATGGATATTTTGTAATCTATACAAGAAAAGGGCACTATAAGGAATATGTTGTCGTCCCCAAATACAAGAAGAACATAATGCTGCTCGGAGATGGAATTAATAAGACTATCATTACTGGAAAACACAATTTTGTCGATGGCTGGACTACTTTCAATTCCTCAACCTTTGCTGTTTCGGGAGAGCGATTTGTGGCCATAGATGTTACCTTCAAGAACACCGCAGGCCCAGAAAAGCACCAAGCAGTGGCTGTAAGAAACAATGCAGACTTCTCCACATTTTACAGATGCAGTTTCGAAGGGTATCAGGACACTCTATATGCACATTCTCTAAGGCAATTCTACAAAGAATGTGACATATACGGAACTGTAGACTTCATCTTCGGCAATGCTGCTGCTGTCTTCCAGAGTTGCAACTTGCCTTGGAAGGTGTACTCTAGGACTGTGGTCATGCAGTCTTATATTGGCCCTCTGATCAGTGCTGTTGGGTGGTTGGAATGGAATGGGACTGTTGGATTAGACACCTTATATTATGGTGAGTTTGAGAATCATGGACCTGGAGCTAATACTAGTATGAGAGTACATTGGCCTGGTTACAGTCTCATGAATGCTACACAAGCTCTGAATTTTACAGTGCTCAACTTCACATTGGGGGACACTTGGTTGCCTGACACAGATATACCCTTCTCTGCAGGTTTAATTGGGAATTAA

Protein Analysis

420

Amino Acids

46.5

Weight (kDa)

5.62

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 7 - 93 8.2e-11 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 158 - 324 7.2e-74 Pectinesterase
Pectinesterase PF01095 325 - 402 9.7e-29 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 179, 1235
AccB7I CCANNNNNTGG 3 cut(s) 118, 557, 799
AccI GTMKAC 3 cut(s) 60, 210, 924
AciI CCGC 1 cut(s) 774
AcoI YGGCCR 3 cut(s) 96, 192, 747
AcsI RAATTY 3 cut(s) 409, 426, 1177
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 493, 986, 1134
AfiI CCNNNNNNNGG 3 cut(s) 118, 557, 799
AgsI TTSAA 5 cut(s) 28, 371, 405, 709, 766
AhlI ACTAGT 1 cut(s) 1121
AjnI CCWGG 3 cut(s) 111, 1108, 1141
AluBI AGCT 8 cut(s) 52, 88, 103, 118, 259, 518, 1115, 1172
AluI AGCT 8 cut(s) 52, 88, 103, 118, 259, 518, 1115, 1172
Alw21I GWGCWC 2 cut(s) 90, 1191
Alw26I GTCTC 1 cut(s) 1157
AlwNI CAGNNNCTG 3 cut(s) 118, 139, 803
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 8 cut(s) 15, 72, 96, 192, 747, 778, 1018, 1139
ApeKI GCWGC 7 cut(s) 103, 201, 256, 518, 637, 944, 947
ApoI RAATTY 3 cut(s) 409, 426, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
AseI ATTAAT 1 cut(s) 654
AspS9I GGNCC 4 cut(s) 130, 779, 1019, 1106
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 431, 1100
AsuII TTCGAA 1 cut(s) 849
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 2 cut(s) 130, 1106
BaeGI GKGCMC 1 cut(s) 591
BalI TGGCCA 2 cut(s) 194, 749
BanII GRGCYC 1 cut(s) 90
BauI CACGAG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 90, 1191
BbvI GCAGC 7 cut(s) 90, 213, 268, 505, 624, 931, 934
BccI CCATC 5 cut(s) 83, 173, 551, 641, 686
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 113, 1110, 1143
BclI TGATCA 1 cut(s) 1026
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 1157
BcuI ACTAGT 1 cut(s) 1121
BfaI CTAG 3 cut(s) 216, 990, 1122
BfmI CTRYAG 3 cut(s) 262, 921, 1242
BfuAI ACCTGC 2 cut(s) 179, 1235
BisI GCNGC 7 cut(s) 104, 202, 257, 519, 638, 945, 948
BlsI GCNGC 7 cut(s) 105, 203, 258, 520, 639, 946, 949
Bme1390I CCNGG 4 cut(s) 113, 359, 1110, 1143
Bme18I GGWCC 2 cut(s) 130, 1106
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 4 cut(s) 130, 779, 1019, 1106
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 4 cut(s) 113, 359, 1110, 1143
BmsI GCATC 1 cut(s) 830
BoxI GACNNNNGTC 1 cut(s) 998
BpiI GAAGAC 1 cut(s) 946
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 1 cut(s) 1131
Bpu14I TTCGAA 1 cut(s) 849
BpuEI CTTGAG 2 cut(s) 74, 293
BpuMI CCSGG 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 2 cut(s) 12, 974
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 557, 799
Bse1I ACTGG 1 cut(s) 676
Bse3DI GCAATG 1 cut(s) 946
BseBI CCWGG 3 cut(s) 113, 1110, 1143
BseDI CCNNGG 2 cut(s) 12, 974
BseLI CCNNNNNNNGG 3 cut(s) 118, 557, 799
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 2 cut(s) 134, 356
BseNI ACTGG 1 cut(s) 676
BseSI GKGCMC 1 cut(s) 591
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 7 cut(s) 90, 213, 268, 505, 624, 931, 934
BsgI GTGCAG 1 cut(s) 516
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 8 cut(s) 17, 74, 98, 194, 749, 780, 1020, 1141
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 90, 1191
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 358
BslFI GGGAC 4 cut(s) 210, 596, 1072, 1220
BslI CCNNNNNNNGG 3 cut(s) 118, 557, 799
BsmAI GTCTC 1 cut(s) 1157
BsmFI GGGAC 4 cut(s) 210, 596, 1072, 1220
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 8 cut(s) 17, 74, 98, 194, 749, 780, 1020, 1141
BsoBI CYCGRG 1 cut(s) 431
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 3 cut(s) 90, 591, 1191
Bsp143I GATC 1 cut(s) 1026
BspACI CCGC 1 cut(s) 774
BspANI GGCC 8 cut(s) 17, 74, 98, 194, 749, 780, 1020, 1141
BspCNI CTCAG 2 cut(s) 135, 355
BspHI TCATGA 1 cut(s) 1155
BspLI GGNNCC 1 cut(s) 363
BspMAI CTGCAG 1 cut(s) 1246
BspMI ACCTGC 2 cut(s) 179, 1235
BspT104I TTCGAA 1 cut(s) 849
BsrDI GCAATG 1 cut(s) 946
BsrI ACTGG 1 cut(s) 676
BssECI CCNNGG 2 cut(s) 12, 974
BssMI GATC 1 cut(s) 1026
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 974
Bst2BI CACGAG 1 cut(s) 220
Bst2UI CCWGG 3 cut(s) 113, 1110, 1143
Bst4CI ACNGT 7 cut(s) 266, 472, 922, 997, 1063, 1151, 1186
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 2 cut(s) 38, 778
BstDEI CTNAG 4 cut(s) 143, 342, 389, 884
BstKTI GATC 1 cut(s) 1029
BstMAI GTCTC 1 cut(s) 1157
BstMBI GATC 1 cut(s) 1026
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 1 cut(s) 1169
BstNI CCWGG 3 cut(s) 113, 1110, 1143
BstPAI GACNNNNGTC 1 cut(s) 998
BstSCI CCNGG 4 cut(s) 111, 357, 1108, 1141
BstSFI CTRYAG 3 cut(s) 262, 921, 1242
BstSLI GKGCMC 1 cut(s) 591
BstV1I GCAGC 7 cut(s) 90, 213, 268, 505, 624, 931, 934
BstV2I GAAGAC 1 cut(s) 946
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 8 cut(s) 17, 74, 98, 194, 749, 780, 1020, 1141
BtsI GCAGTG 2 cut(s) 454, 804
BtsIMutI CAGTG 6 cut(s) 271, 454, 468, 804, 1036, 1191
BveI ACCTGC 2 cut(s) 179, 1235
Cac8I GCNNGC 2 cut(s) 38, 778
CaiI CAGNNNCTG 3 cut(s) 118, 139, 803
CciI TCATGA 1 cut(s) 1155
Cfr13I GGNCC 4 cut(s) 130, 779, 1019, 1106
CseI GACGC 1 cut(s) 17
Csp6I GTAC 3 cut(s) 492, 985, 1133
CviAII CATG 4 cut(s) 488, 1003, 1103, 1156
CviQI GTAC 3 cut(s) 492, 985, 1133
DdeI CTNAG 4 cut(s) 143, 342, 389, 884
DpnI GATC 1 cut(s) 1028
DpnII GATC 1 cut(s) 1026
EaeI YGGCCR 3 cut(s) 96, 192, 747
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Ecl136II GAGCTC 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 974
Eco24I GRGCYC 1 cut(s) 90
Eco47I GGWCC 2 cut(s) 130, 1106
Eco52I CGGCCG 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 3 cut(s) 111, 1108, 1141
EcoT14I CCWWGG 1 cut(s) 974
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 4 cut(s) 491, 1006, 1106, 1159
FaqI GGGAC 4 cut(s) 210, 596, 1072, 1220
FatI CATG 4 cut(s) 487, 1002, 1102, 1155
FbaI TGATCA 1 cut(s) 1026
FblI GTMKAC 3 cut(s) 60, 210, 924
Fnu4HI GCNGC 7 cut(s) 104, 202, 257, 519, 638, 945, 948
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 7 cut(s) 104, 202, 257, 519, 638, 945, 948
FspBI CTAG 3 cut(s) 216, 990, 1122
GluI GCNGC 7 cut(s) 104, 202, 257, 519, 638, 945, 948
GsuI CTGGAG 1 cut(s) 1131
HaeIII GGCC 8 cut(s) 17, 74, 98, 194, 749, 780, 1020, 1141
HapII CCGG 1 cut(s) 358
HgaI GACGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 4 cut(s) 491, 1006, 1106, 1159
HincII GTYRAC 2 cut(s) 61, 211
HindII GTYRAC 2 cut(s) 61, 211
HinfI GANTC 5 cut(s) 136, 223, 481, 527, 1099
HpaII CCGG 1 cut(s) 358
HphI GGTGA 2 cut(s) 431, 1100
Hpy166II GTNNAC 6 cut(s) 61, 124, 211, 485, 925, 985
Hpy188I TCNGA 4 cut(s) 49, 644, 1026, 1176
Hpy188III TCNNGA 9 cut(s) 79, 133, 310, 431, 732, 766, 860, 958, 1156
Hpy8I GTNNAC 6 cut(s) 61, 124, 211, 485, 925, 985
Hpy99I CGWCG 3 cut(s) 11, 62, 85
HpyAV CCTTC 4 cut(s) 772, 845, 973, 1246
HpyCH4III ACNGT 7 cut(s) 266, 472, 922, 997, 1063, 1151, 1186
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 7 cut(s) 533, 818, 843, 875, 966, 1006, 1244
HpyF10VI GCNNNNNNNGC 1 cut(s) 1169
HpyF3I CTNAG 4 cut(s) 143, 342, 389, 884
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 4 cut(s) 491, 1006, 1106, 1159
Ksp22I TGATCA 1 cut(s) 1026
Kzo9I GATC 1 cut(s) 1026
LmnI GCTCC 6 cut(s) 49, 85, 115, 361, 515, 1112
Lsp1109I GCAGC 7 cut(s) 90, 213, 268, 505, 624, 931, 934
LweI GCATC 1 cut(s) 830
MaeI CTAG 3 cut(s) 216, 990, 1122
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 6 cut(s) 154, 283, 347, 757, 905, 1145
MalI GATC 1 cut(s) 1028
MboI GATC 1 cut(s) 1026
MboII GAAGA 6 cut(s) 393, 406, 566, 637, 925, 946
MhlI GDGCHC 3 cut(s) 90, 591, 1191
MlsI TGGCCA 2 cut(s) 194, 749
MluCI AATT 9 cut(s) 409, 426, 651, 682, 709, 890, 1177, 1251, 1258
MluNI TGGCCA 2 cut(s) 194, 749
MlyI GAGTC 4 cut(s) 130, 232, 490, 536
MmeI TCCRAC 3 cut(s) 27, 1026, 1045
MnlI CCTC 8 cut(s) 7, 64, 317, 376, 408, 427, 724, 1032
Mox20I TGGCCA 2 cut(s) 194, 749
MscI TGGCCA 2 cut(s) 194, 749
MseI TTAA 4 cut(s) 462, 654, 1250, 1261
Msp20I TGGCCA 2 cut(s) 194, 749
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 4 cut(s) 113, 359, 1110, 1143
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 3 cut(s) 113, 1110, 1143
MwoI GCNNNNNNNGC 1 cut(s) 1169
NciI CCSGG 1 cut(s) 359
NdeII GATC 1 cut(s) 1026
NlaIII CATG 4 cut(s) 491, 1006, 1106, 1159
NlaIV GGNNCC 1 cut(s) 363
NmeAIII GCCGAG 2 cut(s) 37, 124
NmuCI GTSAC 4 cut(s) 154, 283, 347, 905
NspV TTCGAA 1 cut(s) 849
PaeR7I CTCGAG 1 cut(s) 431
PagI TCATGA 1 cut(s) 1155
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 1 cut(s) 1099
PflFI GACNNNGTC 2 cut(s) 128, 470
PflMI CCANNNNNTGG 3 cut(s) 118, 557, 799
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 7 cut(s) 105, 203, 258, 520, 639, 946, 949
PleI GAGTC 4 cut(s) 130, 231, 489, 535
PpsI GAGTC 4 cut(s) 130, 231, 489, 535
PshAI GACNNNNGTC 1 cut(s) 998
PshBI ATTAAT 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 3 cut(s) 111, 1108, 1141
PspGI CCWGG 3 cut(s) 111, 1108, 1141
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 4 cut(s) 130, 779, 1019, 1106
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 3 cut(s) 118, 139, 803
PsyI GACNNNGTC 2 cut(s) 128, 470
RsaI GTAC 3 cut(s) 493, 986, 1134
RsaNI GTAC 3 cut(s) 492, 985, 1133
SacI GAGCTC 1 cut(s) 90
SalI GTCGAC 2 cut(s) 59, 209
SaqAI TTAA 4 cut(s) 462, 654, 1250, 1261
SatI GCNGC 7 cut(s) 104, 202, 257, 519, 638, 945, 948
Sau3AI GATC 1 cut(s) 1026
Sau96I GGNCC 4 cut(s) 130, 779, 1019, 1106
SchI GAGTC 4 cut(s) 130, 232, 490, 536
ScrFI CCNGG 4 cut(s) 113, 359, 1110, 1143
SduI GDGCHC 3 cut(s) 90, 591, 1191
SfaNI GCATC 1 cut(s) 830
SfcI CTRYAG 3 cut(s) 262, 921, 1242
Sfr274I CTCGAG 1 cut(s) 431
SfuI TTCGAA 1 cut(s) 849
SinI GGWCC 2 cut(s) 130, 1106
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 3 cut(s) 89, 308, 431
SmoI CTYRAG 3 cut(s) 89, 308, 431
SpeI ACTAGT 1 cut(s) 1121
Sse9I AATT 9 cut(s) 409, 426, 651, 682, 709, 890, 1177, 1251, 1258
SsiI CCGC 1 cut(s) 774
SspMI CTAG 3 cut(s) 216, 990, 1122
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 4 cut(s) 111, 357, 1108, 1141
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 7 cut(s) 266, 472, 922, 997, 1063, 1151, 1186
TaiI ACGT 1 cut(s) 60
TaqI TCGA 9 cut(s) 60, 80, 210, 301, 381, 413, 432, 690, 849
TaqII GACCGA 1 cut(s) 20
TasI AATT 9 cut(s) 409, 426, 651, 682, 709, 890, 1177, 1251, 1258
TatI WGTACW 2 cut(s) 984, 1132
TfiI GAWTC 1 cut(s) 1099
Tru1I TTAA 4 cut(s) 462, 654, 1250, 1261
Tru9I TTAA 4 cut(s) 462, 654, 1250, 1261
TscAI CASTG 6 cut(s) 271, 454, 475, 804, 1036, 1191
TseFI GTSAC 4 cut(s) 154, 283, 347, 905
TseI GCWGC 7 cut(s) 103, 201, 256, 518, 637, 944, 947
Tsp45I GTSAC 4 cut(s) 154, 283, 347, 905
TspDTI ATGAA 3 cut(s) 367, 919, 1172
TspGWI ACGGA 4 cut(s) 98, 223, 509, 930
TspRI CASTG 6 cut(s) 271, 454, 475, 804, 1036, 1191
Tth111I GACNNNGTC 2 cut(s) 128, 470
Van91I CCANNNNNTGG 3 cut(s) 118, 557, 799
VpaK11BI GGWCC 2 cut(s) 130, 1106
VspI ATTAAT 1 cut(s) 654
XapI RAATTY 3 cut(s) 409, 426, 1177
XhoI CTCGAG 1 cut(s) 431
XmiI GTMKAC 3 cut(s) 60, 210, 924
XspI CTAG 3 cut(s) 216, 990, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.