Rh1AG113000

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0011631
Physical Location & Seq
Forward (+)
19526745 .. 19531246
4502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG113000.1

Sequence Viewer

Length: 1722 bp
ATGCCGAGACTACCACAATCCTTGCCGCTGTTCCTCCTCCTTGTTGTTCCTCTTCTCTTCTTTTTCTCGGCCGAAGCTGAGTTGCCACCATCACCATCACCCTCCTCCTCTGCTTCCGCCGCGTGCAAGTCCACCCTCTACCCCAAGCTCTGCCGTTCGATTCTCTCCACCATCAGATCCTCCCCCTCCGACCCCTACAACTACGGCAAGTTCTCCATCAAGCAATGCCTCAAAAAGGCACAAAGACTCTCTAAAGCCCTCGACCAATTCCTCCACCACAACAAACGCTCCTCCTCCATGACCGACGCCGAGGCCAACGCTTTCAAGGACTGCCAGCAGTTGTCGGAGCTGAACGTCGACTACTTGGAGGCCATCTCGGCGGAGCTCAAGTCGGCCGAGCTGCTTAACCAGGAGATGGTGGATAAGGTGCAGACTCTACTGAGTGGGATAGTGACAAACCAGCAGACCTGCTACGATGGGCTGGTTGTGGCCAAGAGCAGCATTGTCGACACTAGCACGAGTCCCTTGTCGGTAGCCTTGTCCAATGCCACGCAGCTCTACAGTGTATCGCTTGGGTTGGTCACTCATTCTTTGGCTCGAAACCTCAAGAAGCATAAGAAACGAAACGGCTTTGTTTCTCATGAGGGTCACAAAATCCGGGAGCCACTTGAAACATTCATCGAGGGTCTAAGGAAGAGTTCTTCAAAAAATTTGAGGGGTGAGAGAATTCTCGAGGAAATGGAGAGCAGTGGCATTATAGTTAATGACACTGTCATTGTGAGTCCACATGGTACGGACAACTTCACTTCCATTGGAGCTGCCATTGAGTCTGCACCTAACAACACAGAGCCAGAAGATGGATATTTTGTAATCTATGCAAGAAAAGGGCACTATAAGGAATATGTTGTCGTCCCCAAATATAAGAAGAACATAATGCTGCTCGGAGATGGAATTAATAAGACTATCATTACTGGAAAACACAATTTTGTCGATGGCTGGACTACTTTCAATTCCTCAACCTTTGCTGTTTCGGGAGAGCGATTTGTGGCCATAGATGTTACCTTCAAGAACACCGCAGGTCCAGAAAAGCACCAAGCAGTGGCTGTAAGAAACAATGCAGACTTCTCTACATTTTATAGATGCAGTTTCGAAGGGTATCAGGACACTCTATATGCACATTCTCTAAGGCAATTCTACAAAGAATGTGACATATACGGAACTGTAGACTTCATCTTCGGCAATGCTGCTGCCGTCTTCCAGAGTTGCAACTTGTACGCCCGAAAACCCATGCTGAACCAGAAAAACGCATTCACTGCACAGGGACGAACTGACCCGAATCAAAACACGGGAATCTCAATCCACAACTGCACTATTCAAGCTGCACCAGACTTGGCAATGGACTTGAACTCAACTCTAAATTTTTTGGGAAGGCCTTGGAAGGTGTACTCTAGGACTGTGATCATGCAGTCTTATATTGGCCCTCTGATCAGTGCTGTTGGGTGGTTGGAATGGAATGGGACTGTTGGATTAGACACCTTATATTATGGTGAGTTTGAGAATCATGGACCTGGAGCTAATACTAGTATGAGAGTACATTGGCCTGGTTACAGTCTCATGAATGCTACACAAGCTCTCAATTTTACAGTGCTCAACTTCACATTGGGGGACACTTGGTTGCCTGACACAGATATACCCTTCTCTCCAGGTTTAATTGGGAATTAA

Protein Analysis

573

Amino Acids

63.42

Weight (kDa)

7.6

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 192 1.8e-22 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 258 - 555 2.1e-134 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 476, 1067
AccB7I CCANNNNNTGG 3 cut(s) 415, 857, 1099
AccI GTMKAC 3 cut(s) 357, 507, 1224
AccII CGCG 1 cut(s) 122
AciI CCGC 5 cut(s) 26, 117, 120, 380, 1074
AclWI GGATC 1 cut(s) 171
AcoI YGGCCR 4 cut(s) 69, 393, 489, 1047
AcsI RAATTY 3 cut(s) 709, 726, 1417
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 4 cut(s) 793, 1274, 1445, 1593
AfiI CCNNNNNNNGG 4 cut(s) 235, 415, 857, 1099
AgsI TTSAA 7 cut(s) 325, 671, 705, 1009, 1066, 1376, 1405
AhlI ACTAGT 1 cut(s) 1580
AjnI CCWGG 4 cut(s) 408, 1567, 1600, 1702
Alw21I GWGCWC 2 cut(s) 387, 1650
Alw26I GTCTC 1 cut(s) 1616
AlwI GGATC 1 cut(s) 171
AlwNI CAGNNNCTG 1 cut(s) 1103
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 9 cut(s) 69, 312, 369, 393, 489, 1047, 1430, 1477, 1598
ApeKI GCWGC 8 cut(s) 400, 498, 553, 818, 937, 1244, 1247, 1379
ApoI RAATTY 3 cut(s) 709, 726, 1417
ArsI GACNNNNNNTTYG 2 cut(s) 1217, 1249
AseI ATTAAT 1 cut(s) 954
AspS9I GGNCC 3 cut(s) 1079, 1478, 1565
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 4 cut(s) 84, 90, 731, 1559
AsuII TTCGAA 1 cut(s) 1149
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 2 cut(s) 1079, 1565
BaeGI GKGCMC 1 cut(s) 891
BalI TGGCCA 2 cut(s) 491, 1049
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 1246
Bbv12I GWGCWC 2 cut(s) 387, 1650
BbvI GCAGC 8 cut(s) 387, 510, 565, 805, 924, 1231, 1234, 1366
BceAI ACGGC 4 cut(s) 138, 220, 643, 1235
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BciT130I CCWGG 4 cut(s) 410, 1569, 1602, 1704
BclI TGATCA 2 cut(s) 1458, 1485
BcnI CCSGG 1 cut(s) 659
BcoDI GTCTC 1 cut(s) 1616
BcuI ACTAGT 1 cut(s) 1580
BfaI CTAG 3 cut(s) 513, 1449, 1581
BfmI CTRYAG 2 cut(s) 559, 1221
BfuAI ACCTGC 2 cut(s) 476, 1067
BglI GCCNNNNNGGC 1 cut(s) 377
Bme1390I CCNGG 5 cut(s) 410, 659, 1569, 1602, 1704
Bme18I GGWCC 2 cut(s) 1079, 1565
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 1079, 1478, 1565
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 5 cut(s) 410, 659, 1569, 1602, 1704
BmsI GCATC 1 cut(s) 1130
BpiI GAAGAC 1 cut(s) 1246
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 2 cut(s) 1590, 1686
Bpu14I TTCGAA 1 cut(s) 1149
BpuEI CTTGAG 2 cut(s) 371, 590
BpuMI CCSGG 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 309, 1433
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 374, 404
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 415, 857, 1099
Bse1I ACTGG 1 cut(s) 976
Bse3DI GCAATG 3 cut(s) 230, 1246, 1401
BseBI CCWGG 4 cut(s) 410, 1569, 1602, 1704
BseDI CCNNGG 2 cut(s) 309, 1433
BseLI CCNNNNNNNGG 4 cut(s) 235, 415, 857, 1099
BseMI GCAATG 3 cut(s) 230, 1246, 1401
BseMII CTCAG 2 cut(s) 69, 431
BseNI ACTGG 1 cut(s) 976
BseRI GAGGAG 5 cut(s) 26, 94, 97, 280, 283
BseSI GKGCMC 1 cut(s) 891
BseX3I CGGCCG 2 cut(s) 69, 393
BseXI GCAGC 8 cut(s) 387, 510, 565, 805, 924, 1231, 1234, 1366
BsgI GTGCAG 5 cut(s) 449, 816, 1299, 1351, 1365
Bsh1236I CGCG 1 cut(s) 122
Bsh1285I CGRYCG 2 cut(s) 72, 396
BshFI GGCC 9 cut(s) 71, 314, 371, 395, 491, 1049, 1432, 1479, 1600
BsiEI CGRYCG 2 cut(s) 72, 396
BsiHKAI GWGCWC 2 cut(s) 387, 1650
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 1 cut(s) 658
BslFI GGGAC 5 cut(s) 507, 896, 1335, 1531, 1679
BslI CCNNNNNNNGG 4 cut(s) 235, 415, 857, 1099
BsmAI GTCTC 1 cut(s) 1616
BsmFI GGGAC 5 cut(s) 507, 896, 1335, 1531, 1679
BsmI GAATGC 2 cut(s) 1307, 1624
BsnI GGCC 9 cut(s) 71, 314, 371, 395, 491, 1049, 1432, 1479, 1600
BsoBI CYCGRG 1 cut(s) 731
Bsp119I TTCGAA 1 cut(s) 1149
Bsp1286I GDGCHC 3 cut(s) 387, 891, 1650
Bsp143I GATC 3 cut(s) 176, 1458, 1485
BspACI CCGC 5 cut(s) 26, 117, 120, 380, 1074
BspANI GGCC 9 cut(s) 71, 314, 371, 395, 491, 1049, 1432, 1479, 1600
BspCNI CTCAG 2 cut(s) 70, 432
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 2 cut(s) 640, 1614
BspLI GGNNCC 1 cut(s) 663
BspMI ACCTGC 2 cut(s) 476, 1067
BspPI GGATC 1 cut(s) 171
BspT104I TTCGAA 1 cut(s) 1149
BsrDI GCAATG 3 cut(s) 230, 1246, 1401
BsrI ACTGG 1 cut(s) 976
BssECI CCNNGG 2 cut(s) 309, 1433
BssMI GATC 3 cut(s) 176, 1458, 1485
BssNI GRCGYC 1 cut(s) 306
BssSI CACGAG 1 cut(s) 517
BssT1I CCWWGG 1 cut(s) 1433
Bst2BI CACGAG 1 cut(s) 517
Bst2UI CCWGG 4 cut(s) 410, 1569, 1602, 1704
Bst4CI ACNGT 7 cut(s) 563, 772, 1222, 1456, 1522, 1610, 1645
Bst6I CTCTTC 3 cut(s) 57, 62, 689
BstACI GRCGYC 1 cut(s) 306
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBI TTCGAA 1 cut(s) 1149
BstC8I GCNNGC 2 cut(s) 124, 335
BstDEI CTNAG 4 cut(s) 78, 440, 689, 1184
BstENI CCTNNNNNAGG 1 cut(s) 233
BstFNI CGCG 1 cut(s) 122
BstKTI GATC 3 cut(s) 179, 1461, 1488
BstMAI GTCTC 1 cut(s) 1616
BstMBI GATC 3 cut(s) 176, 1458, 1485
BstMCI CGRYCG 2 cut(s) 72, 396
BstMWI GCNNNNNNNGC 4 cut(s) 119, 377, 1313, 1628
BstNI CCWGG 4 cut(s) 410, 1569, 1602, 1704
BstSCI CCNGG 5 cut(s) 408, 657, 1567, 1600, 1702
BstSFI CTRYAG 2 cut(s) 559, 1221
BstSLI GKGCMC 1 cut(s) 891
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 8 cut(s) 387, 510, 565, 805, 924, 1231, 1234, 1366
BstV2I GAAGAC 1 cut(s) 1246
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BstZI CGGCCG 2 cut(s) 69, 393
BsuRI GGCC 9 cut(s) 71, 314, 371, 395, 491, 1049, 1432, 1479, 1600
BtsI GCAGTG 3 cut(s) 754, 1104, 1311
BtsIMutI CAGTG 7 cut(s) 568, 754, 768, 1104, 1311, 1495, 1650
BveI ACCTGC 2 cut(s) 476, 1067
Cac8I GCNNGC 2 cut(s) 124, 335
CaiI CAGNNNCTG 1 cut(s) 1103
CciI TCATGA 2 cut(s) 640, 1614
Cfr13I GGNCC 3 cut(s) 1079, 1478, 1565
CseI GACGC 1 cut(s) 314
Csp6I GTAC 4 cut(s) 792, 1273, 1444, 1592
CspCI CAANNNNNGTGG 1 cut(s) 38
CviAII CATG 7 cut(s) 298, 641, 788, 1288, 1462, 1562, 1615
CviQI GTAC 4 cut(s) 792, 1273, 1444, 1592
DdeI CTNAG 4 cut(s) 78, 440, 689, 1184
DpnI GATC 3 cut(s) 178, 1460, 1487
DpnII GATC 3 cut(s) 176, 1458, 1485
EaeI YGGCCR 4 cut(s) 69, 393, 489, 1047
EagI CGGCCG 2 cut(s) 69, 393
Eam1104I CTCTTC 3 cut(s) 57, 62, 689
EarI CTCTTC 3 cut(s) 57, 62, 689
EciI GGCGGA 2 cut(s) 106, 395
Ecl136II GAGCTC 1 cut(s) 385
EclXI CGGCCG 2 cut(s) 69, 393
Eco130I CCWWGG 1 cut(s) 1433
Eco147I AGGCCT 1 cut(s) 1432
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 1079, 1565
Eco52I CGGCCG 2 cut(s) 69, 393
Eco53kI GAGCTC 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 4 cut(s) 408, 1567, 1600, 1702
EcoT14I CCWWGG 1 cut(s) 1433
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 1433
FaeI CATG 7 cut(s) 301, 644, 791, 1291, 1465, 1565, 1618
FaqI GGGAC 5 cut(s) 507, 896, 1335, 1531, 1679
FatI CATG 7 cut(s) 297, 640, 787, 1287, 1461, 1561, 1614
FbaI TGATCA 2 cut(s) 1458, 1485
FblI GTMKAC 3 cut(s) 357, 507, 1224
FriOI GRGCYC 1 cut(s) 387
FspBI CTAG 3 cut(s) 513, 1449, 1581
GsuI CTGGAG 2 cut(s) 1590, 1686
HaeIII GGCC 9 cut(s) 71, 314, 371, 395, 491, 1049, 1432, 1479, 1600
HapII CCGG 1 cut(s) 658
HgaI GACGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 7 cut(s) 301, 644, 791, 1291, 1465, 1565, 1618
HincII GTYRAC 2 cut(s) 358, 508
HindII GTYRAC 2 cut(s) 358, 508
HinfI GANTC 9 cut(s) 160, 246, 433, 520, 781, 827, 1336, 1350, 1558
HpaII CCGG 1 cut(s) 658
HphI GGTGA 4 cut(s) 84, 90, 731, 1559
Hpy166II GTNNAC 6 cut(s) 132, 358, 508, 785, 1225, 1444
Hpy188I TCNGA 5 cut(s) 176, 190, 346, 944, 1485
Hpy188III TCNNGA 9 cut(s) 607, 641, 731, 1032, 1066, 1082, 1160, 1258, 1615
Hpy8I GTNNAC 6 cut(s) 132, 358, 508, 785, 1225, 1444
Hpy99I CGWCG 2 cut(s) 308, 359
HpyAV CCTTC 5 cut(s) 1072, 1145, 1422, 1432, 1705
HpyCH4III ACNGT 7 cut(s) 563, 772, 1222, 1456, 1522, 1610, 1645
HpyCH4IV ACGT 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 377, 1313, 1628
HpyF3I CTNAG 4 cut(s) 78, 440, 689, 1184
HpySE526I ACGT 1 cut(s) 354
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 7 cut(s) 301, 644, 791, 1291, 1465, 1565, 1618
Ksp22I TGATCA 2 cut(s) 1458, 1485
Kzo9I GATC 3 cut(s) 176, 1458, 1485
LmnI GCTCC 6 cut(s) 293, 346, 382, 661, 815, 1571
Lsp1109I GCAGC 8 cut(s) 387, 510, 565, 805, 924, 1231, 1234, 1366
LweI GCATC 1 cut(s) 1130
MaeI CTAG 3 cut(s) 513, 1449, 1581
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 6 cut(s) 451, 580, 647, 1057, 1205, 1604
MalI GATC 3 cut(s) 178, 1460, 1487
MboI GATC 3 cut(s) 176, 1458, 1485
MboII GAAGA 8 cut(s) 44, 49, 693, 706, 866, 937, 1225, 1246
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 3 cut(s) 387, 891, 1650
MlsI TGGCCA 2 cut(s) 491, 1049
MluNI TGGCCA 2 cut(s) 491, 1049
MlyI GAGTC 5 cut(s) 240, 427, 529, 790, 836
MmeI TCCRAC 4 cut(s) 213, 324, 1485, 1504
Mox20I TGGCCA 2 cut(s) 491, 1049
MscI TGGCCA 2 cut(s) 491, 1049
MseI TTAA 5 cut(s) 405, 762, 954, 1709, 1720
Msp20I TGGCCA 2 cut(s) 491, 1049
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 1 cut(s) 658
MspR9I CCNGG 5 cut(s) 410, 659, 1569, 1602, 1704
Mva1269I GAATGC 2 cut(s) 1307, 1624
MvaI CCWGG 4 cut(s) 410, 1569, 1602, 1704
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 119, 377, 1313, 1628
NciI CCSGG 1 cut(s) 659
NdeII GATC 3 cut(s) 176, 1458, 1485
NlaIII CATG 7 cut(s) 301, 644, 791, 1291, 1465, 1565, 1618
NlaIV GGNNCC 1 cut(s) 663
NmeAIII GCCGAG 5 cut(s) 30, 47, 334, 356, 421
NmuCI GTSAC 4 cut(s) 451, 580, 647, 1205
NspV TTCGAA 1 cut(s) 1149
PaeR7I CTCGAG 1 cut(s) 731
PagI TCATGA 2 cut(s) 640, 1614
PceI AGGCCT 1 cut(s) 1432
PctI GAATGC 2 cut(s) 1307, 1624
PfeI GAWTC 4 cut(s) 160, 1336, 1350, 1558
PflFI GACNNNGTC 1 cut(s) 770
PflMI CCANNNNNTGG 3 cut(s) 415, 857, 1099
PfoI TCCNGGA 1 cut(s) 657
PleI GAGTC 5 cut(s) 240, 427, 528, 789, 835
PpsI GAGTC 5 cut(s) 240, 427, 528, 789, 835
PshBI ATTAAT 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 4 cut(s) 408, 1567, 1600, 1702
PspGI CCWGG 4 cut(s) 408, 1567, 1600, 1702
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 3 cut(s) 1079, 1478, 1565
PsrI GAACNNNNNNTAC 2 cut(s) 344, 376
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 770
RsaI GTAC 4 cut(s) 793, 1274, 1445, 1593
RsaNI GTAC 4 cut(s) 792, 1273, 1444, 1592
SacI GAGCTC 1 cut(s) 387
SalI GTCGAC 2 cut(s) 356, 506
SaqAI TTAA 5 cut(s) 405, 762, 954, 1709, 1720
Sau3AI GATC 3 cut(s) 176, 1458, 1485
Sau96I GGNCC 3 cut(s) 1079, 1478, 1565
SchI GAGTC 5 cut(s) 240, 427, 529, 790, 836
ScrFI CCNGG 5 cut(s) 410, 659, 1569, 1602, 1704
SduI GDGCHC 3 cut(s) 387, 891, 1650
SfaNI GCATC 1 cut(s) 1130
SfcI CTRYAG 2 cut(s) 559, 1221
Sfr274I CTCGAG 1 cut(s) 731
SfuI TTCGAA 1 cut(s) 1149
SinI GGWCC 2 cut(s) 1079, 1565
SlaI CTCGAG 1 cut(s) 731
SmlI CTYRAG 3 cut(s) 386, 605, 731
SmoI CTYRAG 3 cut(s) 386, 605, 731
SpeI ACTAGT 1 cut(s) 1580
SseBI AGGCCT 1 cut(s) 1432
SsiI CCGC 5 cut(s) 26, 117, 120, 380, 1074
SspMI CTAG 3 cut(s) 513, 1449, 1581
SstI GAGCTC 1 cut(s) 387
StuI AGGCCT 1 cut(s) 1432
StyD4I CCNGG 5 cut(s) 408, 657, 1567, 1600, 1702
StyI CCWWGG 1 cut(s) 1433
TaaI ACNGT 7 cut(s) 563, 772, 1222, 1456, 1522, 1610, 1645
TaiI ACGT 1 cut(s) 357
TaqI TCGA 9 cut(s) 158, 261, 357, 507, 598, 681, 732, 990, 1149
TaqII GACCGA 1 cut(s) 317
TatI WGTACW 2 cut(s) 1443, 1591
TauI GCSGC 2 cut(s) 28, 122
TfiI GAWTC 4 cut(s) 160, 1336, 1350, 1558
Tru1I TTAA 5 cut(s) 405, 762, 954, 1709, 1720
Tru9I TTAA 5 cut(s) 405, 762, 954, 1709, 1720
TscAI CASTG 7 cut(s) 568, 754, 775, 1104, 1318, 1495, 1650
TseFI GTSAC 4 cut(s) 451, 580, 647, 1205
TseI GCWGC 8 cut(s) 400, 498, 553, 818, 937, 1244, 1247, 1379
Tsp45I GTSAC 4 cut(s) 451, 580, 647, 1205
TspDTI ATGAA 3 cut(s) 667, 1219, 1631
TspGWI ACGGA 2 cut(s) 809, 1230
TspRI CASTG 7 cut(s) 568, 754, 775, 1104, 1318, 1495, 1650
Tth111I GACNNNGTC 1 cut(s) 770
Van91I CCANNNNNTGG 3 cut(s) 415, 857, 1099
VpaK11BI GGWCC 2 cut(s) 1079, 1565
VspI ATTAAT 1 cut(s) 954
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 3 cut(s) 709, 726, 1417
XhoI CTCGAG 1 cut(s) 731
XmiI GTMKAC 3 cut(s) 357, 507, 1224
XspI CTAG 3 cut(s) 513, 1449, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.