AT5G04970

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011631
Physical Location & Seq
Reverse (-)
1464079 .. 1467161
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04970.1

Sequence Viewer

Length: 1875 bp
ATGCAAACTCATTCTTCATCTCTCGTGTTCTTGGCTCTTCTCTGTCTGTCATGGGCTTTACTAGTCTCACCAACACGTCCTCCGTCACAACCTCCATCACATCCTCCAATACAGCCTTCGTCACAGCCGCCAACACAGCCTCCGTCACAGCCACCAACGCAGCCTCCAACACAGCCTCCGTCACATCCACCAACACAGCCTCCAACACCGCCACCATCACAGTCTCCATCGCAGCCTTCTCCACTACCACCAAACATCGCTTGTAAATCCACTCCTTACCCAAAGCTCTGCCGCACGATCCTCTCCGCCGTTAAATCATCACCTTCCGATCCTTACCATTACGGAAAGTTCACAATGAAGCAATGCCTCAAACAAGCTCGTCGCCTCTCCAAAGTCATCAACCGCTTTGCCCAGCGCGTGGAAGCTGATCCAGGTACGTCGACGGTGGAAGAGGTCAGTGCGGTGGCTGATTGCGGTGAGCTAGCAGAGCTTAGCGTCGAATACCTTGAAACGGTTACGGAGGAGCTGAAAGCGGCGGAGCTGATGACGGCTGCGCTCGTTGATCGTGTCACGAGTCTTCTCGGTGGCGTTGTGACTAATCAGCAGACGTGCCTCGACGGTCTTGTGGACGCTAAGAGCGGATTTGCGACGGCGATTGGAACGCCGTTAGGGAATCTAACGCGCCTCTACAGTGTGTCGTTGGGGCTTGTGAGTCACGCGCTTAACCGTAATTTGAAGAGGTATAAAGGTTCCAAGGGGAAAATCTTTGGTGGTGGTAACAAACCCGTTCGTGAACCTCTCGAGACTTTGATTAAGGTTTTACGCAAAACATGCGACAAGGGCAAAGATTGTCGGAAAGCTAACAGAAATTTAGGTGAGCTAGGAGAGACGAGCGGTGGATCGATCCTCGTAAGGGAAGCAGTGACCGTCGGTCCATATGAAACCGATAACTTCCCCACCATAACTGAGGCCGTTGCTGCCGCACCTAATCACACATTCCCGGAGCAAGGCTACTTCGTAATCTATGCAAGGGCAGGTCTTTACGAAGAATATGTTGTCATTTCAAATAAAAAAAGAAACATAATGCTTATTGGGGACGGAATCAATAAAACTATCATTTCTGGAAACCACAGTTTTATTGATGGTTGGACAACGTATAATTCTTCGACCTTTGCGGTGGTGGGAGACCGATTTGTTGCGGTTGATGTGACATTCCGAAACACAGCTGGACCAGAGAAGCACCAGGCTGTGGCCGTAAGAAACAATGCGGATGGATCGACATTTTATCGATGTAGTTTTGAAGGCTATCAAGATACTCTTTACGTTCATTCTTTGAGACAGTTCTATCGTGAATGTGATATATATGGGACCATAGATTTTATATTCGGAAATGCAGCTGCCATATTTCAAAACTGCAACATATATGCCCGAAAACCGATGGCAAATCAGAAGAATGCAGTGACAGCCCATGGAAGAACTGACCCAAACCAGAAAACTGGAATCTCCATCATTAATTGTACTATTGGAGCTGCCCCGGACCTCGCTGCTGACCCCAAGTCGACCATGACATTCCTTGGGCGGCCATGGAAGCCTTACTCGAGGACGGTCTACATACAATCATACATCAGCGATGTTGTCCAACCGGTAGGGTGGCTGGAATGGAATGGTACAACCGGGCTAGACACGATCTCCTATGGTGAATATGACAATTTTGGACCGGGGGCTGATACAAGTAAAAGGGTCCAATGGTCAGGATATAGCCTATTGAACTTGGTACAAGCCATGAACTTCACCGTATATAATTTCACTTTGGGAGACACTTGGTTGCCTCAGACTGACATTCCCTTCTACGGCGGTTTGCTTCACACCGAATGA

Protein Analysis

624

Amino Acids

68.15

Weight (kDa)

8.44

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 87 - 235 4.3e-21 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 308 - 605 2.5e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1025
AccB7I CCANNNNNTGG 2 cut(s) 418, 1249
AccBSI CCGCTC 2 cut(s) 639, 894
AccI GTMKAC 3 cut(s) 440, 1559, 1608
AccII CGCG 3 cut(s) 417, 682, 719
AclWI GGATC 6 cut(s) 292, 323, 422, 898, 907, 1282
AcoI YGGCCR 2 cut(s) 1251, 1580
AcsI RAATTY 1 cut(s) 868
AfaI GTAC 4 cut(s) 436, 1519, 1669, 1776
AfiI CCNNNNNNNGG 6 cut(s) 418, 511, 913, 1007, 1249, 1850
AflIII ACRYGT 1 cut(s) 74
AgeI ACCGGT 1 cut(s) 1642
AgsI TTSAA 6 cut(s) 509, 736, 1065, 1301, 1409, 1768
AhdI GACNNNNNGTC 2 cut(s) 930, 1555
AhlI ACTAGT 1 cut(s) 61
AjiI CACGTC 2 cut(s) 77, 609
AjnI CCWGG 2 cut(s) 430, 1242
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
Alw26I GTCTC 7 cut(s) 70, 228, 797, 881, 1179, 1330, 1809
AlwI GGATC 6 cut(s) 292, 323, 422, 898, 907, 1282
Ama87I CYCGRG 2 cut(s) 800, 1597
AoxI GGCC 3 cut(s) 969, 1251, 1580
ApeKI GCWGC 8 cut(s) 160, 232, 551, 977, 1394, 1397, 1529, 1544
ApoI RAATTY 1 cut(s) 868
AseI ATTAAT 1 cut(s) 1512
AsiGI ACCGGT 1 cut(s) 1642
AspLEI GCGC 4 cut(s) 417, 556, 684, 721
AspS9I GGNCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
AsuC2I CCSGG 4 cut(s) 1001, 1535, 1675, 1719
AsuHPI GGTGA 6 cut(s) 60, 312, 488, 887, 1709, 1783
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 2 cut(s) 800, 1597
AvaII GGWCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
BauI CACGAG 2 cut(s) 23, 571
BbsI GAAGAC 1 cut(s) 569
BbvI GCAGC 8 cut(s) 172, 244, 538, 964, 1384, 1406, 1516, 1531
BccI CCATC 7 cut(s) 103, 223, 235, 1136, 1265, 1432, 1514
BceAI ACGGC 7 cut(s) 293, 564, 649, 666, 956, 1238, 1867
BciT130I CCWGG 2 cut(s) 432, 1244
BcnI CCSGG 4 cut(s) 1001, 1535, 1675, 1719
BcoDI GTCTC 7 cut(s) 70, 228, 797, 881, 1179, 1330, 1809
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 4 cut(s) 62, 482, 881, 1679
BfmI CTRYAG 1 cut(s) 688
BfuAI ACCTGC 1 cut(s) 1025
BlpI GCTNAGC 1 cut(s) 491
Bme1390I CCNGG 6 cut(s) 432, 1001, 1244, 1535, 1675, 1719
Bme18I GGWCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
BmeRI GACNNNNNGTC 2 cut(s) 930, 1555
BmeT110I CYCGRG 2 cut(s) 800, 1597
BmgBI CACGTC 2 cut(s) 77, 609
BmgT120I GGNCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
BmiI GGNNCC 3 cut(s) 751, 1369, 1742
BmrFI CCNGG 6 cut(s) 432, 1001, 1244, 1535, 1675, 1719
BmtI GCTAGC 1 cut(s) 485
BpiI GAAGAC 1 cut(s) 569
Bpu1102I GCTNAGC 1 cut(s) 491
BpuMI CCSGG 4 cut(s) 1001, 1535, 1675, 1719
Bsa29I ATCGAT 2 cut(s) 902, 1288
BsaI GGTCTC 1 cut(s) 1179
BsaJI CCNNGG 6 cut(s) 753, 1468, 1533, 1573, 1583, 1718
BsaWI WCCGGW 1 cut(s) 1642
Bsc4I CCNNNNNNNGG 6 cut(s) 418, 511, 913, 1007, 1249, 1850
Bse118I RCCGGY 1 cut(s) 1642
Bse1I ACTGG 1 cut(s) 1501
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 2 cut(s) 432, 1244
BseCI ATCGAT 2 cut(s) 902, 1288
BseDI CCNNGG 6 cut(s) 753, 1468, 1533, 1573, 1583, 1718
BseGI GGATG 3 cut(s) 100, 184, 1276
BseLI CCNNNNNNNGG 6 cut(s) 418, 511, 913, 1007, 1249, 1850
BseMI GCAATG 1 cut(s) 368
BseMII CTCAG 2 cut(s) 957, 1844
BseNI ACTGG 1 cut(s) 1501
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 8 cut(s) 172, 244, 538, 964, 1384, 1406, 1516, 1531
BseYI CCCAGC 1 cut(s) 411
Bsh1236I CGCG 3 cut(s) 417, 682, 719
BshFI GGCC 3 cut(s) 971, 1253, 1582
BshTI ACCGGT 1 cut(s) 1642
BshVI ATCGAT 2 cut(s) 902, 1288
BsiHKCI CYCGRG 2 cut(s) 800, 1597
BsiSI CCGG 5 cut(s) 1001, 1535, 1643, 1674, 1718
BslFI GGGAC 2 cut(s) 1109, 1381
BslI CCNNNNNNNGG 6 cut(s) 418, 511, 913, 1007, 1249, 1850
BsmAI GTCTC 7 cut(s) 70, 228, 797, 881, 1179, 1330, 1809
BsmBI CGTCTC 1 cut(s) 881
BsmFI GGGAC 2 cut(s) 1109, 1381
BsmI GAATGC 1 cut(s) 1459
BsnI GGCC 3 cut(s) 971, 1253, 1582
Bso31I GGTCTC 1 cut(s) 1179
BsoBI CYCGRG 2 cut(s) 800, 1597
Bsp143I GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
Bsp1720I GCTNAGC 1 cut(s) 491
Bsp19I CCATGG 2 cut(s) 1468, 1583
BspANI GGCC 3 cut(s) 971, 1253, 1582
BspCNI CTCAG 2 cut(s) 958, 1843
BspDI ATCGAT 2 cut(s) 902, 1288
BspFNI CGCG 3 cut(s) 417, 682, 719
BspLI GGNNCC 3 cut(s) 751, 1369, 1742
BspMI ACCTGC 1 cut(s) 1025
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 6 cut(s) 292, 323, 422, 898, 907, 1282
BspQI GCTCTTC 1 cut(s) 42
BspTNI GGTCTC 1 cut(s) 1179
BsrBI CCGCTC 2 cut(s) 639, 894
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 1642
BsrI ACTGG 1 cut(s) 1501
BssAI RCCGGY 1 cut(s) 1642
BssECI CCNNGG 6 cut(s) 753, 1468, 1533, 1573, 1583, 1718
BssMI GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
BssSI CACGAG 2 cut(s) 23, 571
BssT1I CCWWGG 4 cut(s) 753, 1468, 1573, 1583
Bst2BI CACGAG 2 cut(s) 23, 571
Bst2UI CCWGG 2 cut(s) 432, 1244
Bst6I CTCTTC 3 cut(s) 42, 444, 731
BstAPI GCANNNNNTGC 1 cut(s) 831
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 4 cut(s) 491, 633, 966, 1830
BstDSI CCRYGG 2 cut(s) 1468, 1583
BstF5I GGATG 3 cut(s) 100, 184, 1276
BstFNI CGCG 3 cut(s) 417, 682, 719
BstHHI GCGC 4 cut(s) 417, 556, 684, 721
BstKTI GATC 8 cut(s) 300, 331, 430, 565, 902, 906, 1277, 1689
BstMAI GTCTC 7 cut(s) 70, 228, 797, 881, 1179, 1330, 1809
BstMBI GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
BstMWI GCNNNNNNNGC 8 cut(s) 136, 157, 487, 831, 840, 977, 1463, 1588
BstNI CCWGG 2 cut(s) 432, 1244
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 6 cut(s) 430, 999, 1242, 1533, 1673, 1717
BstSFI CTRYAG 1 cut(s) 688
BstUI CGCG 3 cut(s) 417, 682, 719
BstV1I GCAGC 8 cut(s) 172, 244, 538, 964, 1384, 1406, 1516, 1531
BstV2I GAAGAC 1 cut(s) 569
BstXI CCANNNNNNTGG 1 cut(s) 1496
Bsu15I ATCGAT 2 cut(s) 902, 1288
BsuRI GGCC 3 cut(s) 971, 1253, 1582
BsuTUI ATCGAT 2 cut(s) 902, 1288
BtgI CCRYGG 2 cut(s) 1468, 1583
BtgZI GCGATG 3 cut(s) 213, 241, 1644
BtrI CACGTC 2 cut(s) 77, 609
BtsCI GGATG 3 cut(s) 100, 184, 1276
BtsI GCAGTG 2 cut(s) 927, 1464
BtsIMutI CAGTG 4 cut(s) 463, 697, 927, 1464
BveI ACCTGC 1 cut(s) 1025
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 4 cut(s) 417, 556, 684, 721
Cfr10I RCCGGY 1 cut(s) 1642
Cfr13I GGNCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
ClaI ATCGAT 2 cut(s) 902, 1288
CseI GACGC 2 cut(s) 484, 638
Csp6I GTAC 4 cut(s) 435, 1518, 1668, 1775
CspAI ACCGGT 1 cut(s) 1642
CviAII CATG 6 cut(s) 51, 831, 1469, 1564, 1584, 1783
CviQI GTAC 4 cut(s) 435, 1518, 1668, 1775
DdeI CTNAG 4 cut(s) 491, 633, 966, 1830
DpnI GATC 8 cut(s) 299, 330, 429, 564, 901, 905, 1276, 1688
DpnII GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
DriI GACNNNNNGTC 2 cut(s) 930, 1555
EaeI YGGCCR 2 cut(s) 1251, 1580
Eam1104I CTCTTC 3 cut(s) 42, 444, 731
Eam1105I GACNNNNNGTC 2 cut(s) 930, 1555
EarI CTCTTC 3 cut(s) 42, 444, 731
EciI GGCGGA 2 cut(s) 295, 551
Eco130I CCWWGG 4 cut(s) 753, 1468, 1573, 1583
Eco31I GGTCTC 1 cut(s) 1179
Eco47I GGWCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
Eco88I CYCGRG 2 cut(s) 800, 1597
EcoRII CCWGG 2 cut(s) 430, 1242
EcoT14I CCWWGG 4 cut(s) 753, 1468, 1573, 1583
ErhI CCWWGG 4 cut(s) 753, 1468, 1573, 1583
Esp3I CGTCTC 1 cut(s) 881
FaeI CATG 6 cut(s) 54, 834, 1472, 1567, 1587, 1786
FalI AAGNNNNNCTT 2 cut(s) 1302, 1334
FaqI GGGAC 2 cut(s) 1109, 1381
FatI CATG 6 cut(s) 50, 830, 1468, 1563, 1583, 1782
FauNDI CATATG 1 cut(s) 937
FblI GTMKAC 3 cut(s) 440, 1559, 1608
FokI GGATG 3 cut(s) 87, 171, 1283
FspBI CTAG 4 cut(s) 62, 482, 881, 1679
GlaI GCGC 4 cut(s) 416, 555, 683, 720
GsaI CCCAGC 1 cut(s) 415
HaeIII GGCC 3 cut(s) 971, 1253, 1582
HapII CCGG 5 cut(s) 1001, 1535, 1643, 1674, 1718
HgaI GACGC 2 cut(s) 484, 638
HhaI GCGC 4 cut(s) 417, 556, 684, 721
Hin1II CATG 6 cut(s) 54, 834, 1472, 1567, 1587, 1786
Hin6I GCGC 4 cut(s) 415, 554, 682, 719
HinP1I GCGC 4 cut(s) 415, 554, 682, 719
HincII GTYRAC 2 cut(s) 441, 1560
HindII GTYRAC 2 cut(s) 441, 1560
HinfI GANTC 5 cut(s) 574, 673, 712, 1101, 1500
HpaII CCGG 5 cut(s) 1001, 1535, 1643, 1674, 1718
HphI GGTGA 6 cut(s) 60, 312, 488, 887, 1709, 1783
Hpy166II GTNNAC 6 cut(s) 351, 441, 628, 794, 1560, 1609
Hpy188I TCNGA 6 cut(s) 328, 855, 1217, 1388, 1449, 1833
Hpy188III TCNNGA 8 cut(s) 571, 791, 800, 802, 1122, 1310, 1349, 1752
Hpy8I GTNNAC 6 cut(s) 351, 441, 628, 794, 1560, 1609
Hpy99I CGWCG 7 cut(s) 384, 442, 445, 500, 620, 652, 932
HpyAV CCTTC 5 cut(s) 126, 246, 333, 1295, 1855
HpyCH4IV ACGT 5 cut(s) 76, 437, 608, 1154, 1323
HpyCH4V TGCA 5 cut(s) 4, 1028, 1394, 1416, 1457
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 157, 487, 831, 840, 977, 1463, 1588
HpyF3I CTNAG 4 cut(s) 491, 633, 966, 1830
HpySE526I ACGT 5 cut(s) 76, 437, 608, 1154, 1323
Hsp92II CATG 6 cut(s) 54, 834, 1472, 1567, 1587, 1786
HspAI GCGC 4 cut(s) 415, 554, 682, 719
Kzo9I GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 4 cut(s) 523, 538, 1003, 1526
Lsp1109I GCAGC 8 cut(s) 172, 244, 538, 964, 1384, 1406, 1516, 1531
MaeI CTAG 4 cut(s) 62, 482, 881, 1679
MaeII ACGT 5 cut(s) 76, 437, 608, 1154, 1323
MalI GATC 8 cut(s) 299, 330, 429, 564, 901, 905, 1276, 1688
MbiI CCGCTC 2 cut(s) 639, 894
MboI GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
MboII GAAGA 9 cut(s) 6, 29, 461, 569, 748, 1058, 1155, 1462, 1485
MluCI AATT 6 cut(s) 730, 868, 1159, 1513, 1708, 1801
MlyI GAGTC 2 cut(s) 583, 721
MmeI TCCRAC 5 cut(s) 191, 227, 833, 1127, 1663
MseI TTAA 4 cut(s) 312, 723, 813, 1512
MspA1I CMGCKG 2 cut(s) 1226, 1397
MspI CCGG 5 cut(s) 1001, 1535, 1643, 1674, 1718
MspR9I CCNGG 6 cut(s) 432, 1001, 1244, 1535, 1675, 1719
Mva1269I GAATGC 1 cut(s) 1459
MvaI CCWGG 2 cut(s) 432, 1244
MvnI CGCG 3 cut(s) 417, 682, 719
MwoI GCNNNNNNNGC 8 cut(s) 136, 157, 487, 831, 840, 977, 1463, 1588
NciI CCSGG 4 cut(s) 1001, 1535, 1675, 1719
NcoI CCATGG 2 cut(s) 1468, 1583
NdeI CATATG 1 cut(s) 937
NdeII GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 6 cut(s) 54, 834, 1472, 1567, 1587, 1786
NlaIV GGNNCC 3 cut(s) 751, 1369, 1742
NspI RCATGY 1 cut(s) 834
PaeR7I CTCGAG 2 cut(s) 800, 1597
PciSI GCTCTTC 1 cut(s) 42
PctI GAATGC 1 cut(s) 1459
PfeI GAWTC 3 cut(s) 673, 1101, 1500
PflMI CCANNNNNTGG 2 cut(s) 418, 1249
PfoI TCCNGGA 1 cut(s) 999
PinAI ACCGGT 1 cut(s) 1642
PleI GAGTC 2 cut(s) 582, 720
PpsI GAGTC 2 cut(s) 582, 720
PshBI ATTAAT 1 cut(s) 1512
Psp6I CCWGG 2 cut(s) 430, 1242
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 2 cut(s) 430, 1242
PspN4I GGNNCC 3 cut(s) 751, 1369, 1742
PspPI GGNCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
PspXI VCTCGAGB 1 cut(s) 1597
PvuII CAGCTG 2 cut(s) 1226, 1397
RsaI GTAC 4 cut(s) 436, 1519, 1669, 1776
RsaNI GTAC 4 cut(s) 435, 1518, 1668, 1775
SalI GTCGAC 2 cut(s) 439, 1558
SapI GCTCTTC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 312, 723, 813, 1512
Sau3AI GATC 8 cut(s) 297, 328, 427, 562, 899, 903, 1274, 1686
Sau96I GGNCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
SchI GAGTC 2 cut(s) 583, 721
ScrFI CCNGG 6 cut(s) 432, 1001, 1244, 1535, 1675, 1719
SfcI CTRYAG 1 cut(s) 688
Sfr274I CTCGAG 2 cut(s) 800, 1597
SgrDI CGTCGACG 1 cut(s) 439
SinI GGWCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
SlaI CTCGAG 2 cut(s) 800, 1597
SmlI CTYRAG 2 cut(s) 800, 1597
SmoI CTYRAG 2 cut(s) 800, 1597
SpeI ACTAGT 1 cut(s) 61
Sse9I AATT 6 cut(s) 730, 868, 1159, 1513, 1708, 1801
SspMI CTAG 4 cut(s) 62, 482, 881, 1679
StyD4I CCNGG 6 cut(s) 430, 999, 1242, 1533, 1673, 1717
StyI CCWWGG 4 cut(s) 753, 1468, 1573, 1583
TaiI ACGT 5 cut(s) 79, 440, 611, 1157, 1326
TaqII GACCGA 2 cut(s) 920, 1203
TasI AATT 6 cut(s) 730, 868, 1159, 1513, 1708, 1801
TatI WGTACW 1 cut(s) 1517
TauI GCSGC 5 cut(s) 130, 294, 536, 983, 1582
TfiI GAWTC 3 cut(s) 673, 1101, 1500
Tru1I TTAA 4 cut(s) 312, 723, 813, 1512
Tru9I TTAA 4 cut(s) 312, 723, 813, 1512
TscAI CASTG 4 cut(s) 463, 697, 927, 1464
TseI GCWGC 8 cut(s) 160, 232, 551, 977, 1394, 1397, 1529, 1544
TspDTI ATGAA 5 cut(s) 6, 371, 954, 1316, 1799
TspGWI ACGGA 6 cut(s) 72, 132, 168, 357, 533, 1113
TspRI CASTG 4 cut(s) 463, 697, 927, 1464
Van91I CCANNNNNTGG 2 cut(s) 418, 1249
VpaK11BI GGWCC 6 cut(s) 932, 1229, 1368, 1537, 1715, 1741
VspI ATTAAT 1 cut(s) 1512
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 834
XhoI CTCGAG 2 cut(s) 800, 1597
XmiI GTMKAC 3 cut(s) 440, 1559, 1608
XspI CTAG 4 cut(s) 62, 482, 881, 1679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.