MD02G1266500.v1.1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011631
Physical Location & Seq
Reverse (-)
32056283 .. 32061797
5515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1266500.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGCCACGGCTACGCTCCTCTCCTTTCTTCCACCTCCTTTCTCTATCCCTCTTTTCACTACTTCTGCTAACCTCTGGTGCTGCTGCATCATCCTCCTCATCATCCTCTTCAGCATGCAAGTCCACCCTCTACCCAAAGCTCTGCCGTTCGATGCTCTCCACCATTCGCTCCTCACCGTCCGATCCCTACAGCTACGGCAAGTTCTCCATCAAACAATGCCTGAAACAGGCACGCAAAACCTCCACAACAATTAACCACTTCCTTGGAAAAACCAAGCACAAGTCGTATTTCATAAGCGACAGCGAGTCAAACGCGCTGGAGGACTGCCAGCATTTCTCGGAGCTGAACGTGGAGCACTTGGAGGCGATCTCGACAGAGCTCAAGTCCGCCGAGACGCTGAACGAGGAACTGGTGGACAAGGTGCAGACGTTGCTGAGCGGCATTGTGACCAACCAGCAGACTTGTTACGATGGGCTTGTGGTGTCTAAGAGCAGCGTTTTGGGAAACACCACGGCTCTGGCCGTGGCCTTCTCTAATGTTACGCAGCTCTATAGTGTGTCGCTTGGGTTGGTCACGCATTCCTTGGCTCGTAATCTCAAAAAGCATAAGAAAGGCAGTGAGGGCTCCGGCACCAAAAATGTGCACAAATTCCGGGTATCACTTGAAACTTTCATTAAGGCACTTCGGAAAAGTTCTGGCAAAAACTCGACGACTTCCGAAAGAGGTGAGAGGATTCTTGAAGAAATGGAGAGCAGTGGCATTCTGGTTAACGACACAGTGATTGTGGGTCCATATGGGACGGACAACTTTACATCGATCGGAGATGCTATTACTTCTGCTCCCGAGAAGTTGAAGCCTGAAGATGGCTACTTTGTGATCTATGTTAGAGGAGGATGCTATCATGAGTACATTGTCATCGCCAAACACAAGACTAACATAATGCTGCTCGGAGATGGTATTAATAGCACTGTCATTACCGGAAACCATAGTTTCGTCGATGATTGGACTACCTTCAACTCCTCAACCTTTGCTGTTTCTGCGGAACGATTTGTGGCCGTAGATATTACATTCAGGAACACTGCAGGTCCAGAAAAGCACCAAGCGGTAGCTGTAAGAAACAATGCTGACCTCTCCACATTTTACAGGTGCAGCTTCGAAGGCTATCAGGACACTCTATACGTACATTCCCTGAGGCAGTTCTATAAGGAATGTGACATATATGGAACTGTAGATTTCATTTTTGGAAATGCTGCTGCTGTCTTCCAGAGTTGCAATTTATACGCTCGAAAACCGATGCCCAACCAGAAAAACGCCTTCACAGCCCAAGGACGAACTGACCCGAATCAGAACACCGGAATCTCCATCCACAACTGCACAATTGAAGCTGCGCCAGACTTGGCAGCGGACCTCAACTCCACGTTAAATTTTCTGGGAAGGCCTTGGAAGGTGTTTTCTAGGACTGTCATCATGCAGTCTTATATTGGCTCTCTGATCAGCTCAGTTGGATGGTTGGAATGGAATGGGACGGTTGGATTGGACACACTGTTTTACGGTGAGTTTCAGAATCACGGGCCGGGGGCAAACACGACGAAGAGAGTACAATGGCCTGGTTATAGTCTGATGAATGCTACTCAAGCCTTGAATTTCACTGTCAGTAATTTCACATTGGGTGATACTTGGTTGCCCGACACCGATATACCCTTCTATGGAGGGTTGCTGAATTAG

Protein Analysis

575

Amino Acids

63.14

Weight (kDa)

7.95

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 36 - 189 6.7e-23 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 260 - 557 1.1e-135 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1073
AccB1I GGYRCC 1 cut(s) 629
AccBSI CCGCTC 1 cut(s) 438
AccII CGCG 1 cut(s) 314
AciI CCGC 5 cut(s) 387, 438, 1040, 1103, 1403
AclWI GGATC 1 cut(s) 176
AcoI YGGCCR 2 cut(s) 519, 1053
AcsI RAATTY 3 cut(s) 647, 1423, 1642
AcuI CTGAAG 2 cut(s) 93, 879
AfaI GTAC 3 cut(s) 908, 1182, 1599
AfiI CCNNNNNNNGG 3 cut(s) 226, 863, 1706
AgsI TTSAA 6 cut(s) 665, 740, 853, 1015, 1382, 1642
AhdI GACNNNNNGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 1606
AluBI AGCT 9 cut(s) 139, 192, 343, 379, 547, 1109, 1152, 1385, 1497
AluI AGCT 9 cut(s) 139, 192, 343, 379, 547, 1109, 1152, 1385, 1497
Alw21I GWGCWC 3 cut(s) 357, 381, 645
Alw26I GTCTC 1 cut(s) 386
Alw44I GTGCAC 1 cut(s) 641
AlwI GGATC 1 cut(s) 176
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 6 cut(s) 519, 525, 1053, 1436, 1571, 1604
ApaLI GTGCAC 1 cut(s) 641
ApoI RAATTY 3 cut(s) 647, 1423, 1642
AseI ATTAAT 1 cut(s) 960
AspLEI GCGC 2 cut(s) 316, 1390
AspS9I GGNCC 4 cut(s) 788, 1085, 1405, 1571
AsuC2I CCSGG 2 cut(s) 653, 1575
AsuHPI GGTGA 4 cut(s) 165, 737, 1565, 1682
AsuII TTCGAA 1 cut(s) 1155
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 3 cut(s) 788, 1085, 1405
AxyI CCTNAGG 1 cut(s) 1190
BaeGI GKGCMC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 2 cut(s) 381, 626
BbsI GAAGAC 1 cut(s) 1252
Bbv12I GWGCWC 3 cut(s) 357, 381, 645
BccI CCATC 6 cut(s) 215, 464, 857, 947, 1370, 1500
BceAI ACGGC 6 cut(s) 23, 129, 211, 506, 528, 1040
BciT130I CCWGG 1 cut(s) 1608
BclI TGATCA 1 cut(s) 1491
BcnI CCSGG 2 cut(s) 653, 1575
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 1 cut(s) 1455
BfmI CTRYAG 4 cut(s) 187, 550, 1080, 1227
BfuAI ACCTGC 1 cut(s) 1073
BlpI GCTNAGC 1 cut(s) 434
Bme1390I CCNGG 3 cut(s) 653, 1575, 1608
Bme18I GGWCC 3 cut(s) 788, 1085, 1405
BmeRI GACNNNNNGTC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 4 cut(s) 788, 1085, 1405, 1571
BmiI GGNNCC 3 cut(s) 625, 631, 789
BmrFI CCNGG 3 cut(s) 653, 1575, 1608
BmsI GCATC 5 cut(s) 95, 141, 814, 884, 1284
BpiI GAAGAC 1 cut(s) 1252
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 338
Bpu1102I GCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 1 cut(s) 1155
BpuEI CTTGAG 2 cut(s) 365, 1617
BpuMI CCSGG 2 cut(s) 653, 1575
Bsa29I ATCGAT 1 cut(s) 815
BsaAI YACGTR 1 cut(s) 1180
BsaJI CCNNGG 8 cut(s) 5, 262, 510, 522, 582, 1324, 1439, 1574
BsaWI WCCGGW 2 cut(s) 977, 1352
BsaXI ACNNNNNCTCC 4 cut(s) 823, 853, 1397, 1427
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 863, 1706
Bse1I ACTGG 1 cut(s) 414
Bse21I CCTNAGG 1 cut(s) 1190
BseBI CCWGG 1 cut(s) 1608
BseCI ATCGAT 1 cut(s) 815
BseDI CCNNGG 8 cut(s) 5, 262, 510, 522, 582, 1324, 1439, 1574
BseGI GGATG 5 cut(s) 89, 101, 899, 1362, 1511
BseLI CCNNNNNNNGG 3 cut(s) 226, 863, 1706
BseMII CTCAG 3 cut(s) 425, 1181, 1512
BseNI ACTGG 1 cut(s) 414
BseRI GAGGAG 5 cut(s) 7, 85, 160, 903, 1009
BseSI GKGCMC 1 cut(s) 645
BsgI GTGCAG 3 cut(s) 443, 1168, 1357
Bsh1236I CGCG 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 819
BshFI GGCC 6 cut(s) 521, 527, 1055, 1438, 1573, 1606
BshNI GGYRCC 1 cut(s) 629
BshVI ATCGAT 1 cut(s) 815
BsiEI CGRYCG 1 cut(s) 819
BsiHKAI GWGCWC 3 cut(s) 357, 381, 645
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 5 cut(s) 627, 652, 978, 1353, 1574
BslFI GGGAC 2 cut(s) 811, 1537
BslI CCNNNNNNNGG 3 cut(s) 226, 863, 1706
BsmAI GTCTC 1 cut(s) 386
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 811, 1537
BsmI GAATGC 3 cut(s) 577, 759, 1630
BsnI GGCC 6 cut(s) 521, 527, 1055, 1438, 1573, 1606
BsoBI CYCGRG 1 cut(s) 842
Bsp119I TTCGAA 1 cut(s) 1155
Bsp1286I GDGCHC 4 cut(s) 357, 381, 626, 645
Bsp143I GATC 5 cut(s) 181, 366, 816, 876, 1491
Bsp1720I GCTNAGC 1 cut(s) 434
BspACI CCGC 5 cut(s) 387, 438, 1040, 1103, 1403
BspANI GGCC 6 cut(s) 521, 527, 1055, 1438, 1573, 1606
BspCNI CTCAG 3 cut(s) 426, 1182, 1511
BspDI ATCGAT 1 cut(s) 815
BspFNI CGCG 1 cut(s) 314
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 3 cut(s) 625, 631, 789
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 1 cut(s) 1073
BspPI GGATC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 1155
BspT107I GGYRCC 1 cut(s) 629
BsrBI CCGCTC 1 cut(s) 438
BsrI ACTGG 1 cut(s) 414
BssECI CCNNGG 8 cut(s) 5, 262, 510, 522, 582, 1324, 1439, 1574
BssMI GATC 5 cut(s) 181, 366, 816, 876, 1491
BssT1I CCWWGG 4 cut(s) 262, 582, 1324, 1439
Bst2UI CCWGG 1 cut(s) 1608
Bst4CI ACNGT 9 cut(s) 177, 778, 970, 1228, 1462, 1528, 1545, 1553, 1651
Bst6I CTCTTC 2 cut(s) 112, 1586
BstAPI GCANNNNNTGC 1 cut(s) 430
BstBAI YACGTR 1 cut(s) 1180
BstBI TTCGAA 1 cut(s) 1155
BstC8I GCNNGC 3 cut(s) 115, 232, 329
BstDEI CTNAG 4 cut(s) 434, 486, 1190, 1498
BstDSI CCRYGG 3 cut(s) 5, 510, 522
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 5 cut(s) 89, 101, 899, 1362, 1511
BstFNI CGCG 1 cut(s) 314
BstHHI GCGC 2 cut(s) 316, 1390
BstKTI GATC 5 cut(s) 184, 369, 819, 879, 1494
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 5 cut(s) 181, 366, 816, 876, 1491
BstMCI CGRYCG 1 cut(s) 819
BstMWI GCNNNNNNNGC 6 cut(s) 430, 621, 1037, 1158, 1319, 1634
BstNI CCWGG 1 cut(s) 1608
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 3 cut(s) 651, 1573, 1606
BstSFI CTRYAG 4 cut(s) 187, 550, 1080, 1227
BstSLI GKGCMC 1 cut(s) 645
BstSNI TACGTA 1 cut(s) 1180
BstUI CGCG 1 cut(s) 314
BstV2I GAAGAC 1 cut(s) 1252
BstXI CCANNNNNNTGG 2 cut(s) 263, 517
Bsu15I ATCGAT 1 cut(s) 815
Bsu36I CCTNAGG 1 cut(s) 1190
BsuRI GGCC 6 cut(s) 521, 527, 1055, 1438, 1573, 1606
BsuTUI ATCGAT 1 cut(s) 815
BtgI CCRYGG 3 cut(s) 5, 510, 522
BtgZI GCGATG 1 cut(s) 901
BtsCI GGATG 5 cut(s) 89, 101, 899, 1362, 1511
BtsI GCAGTG 3 cut(s) 622, 760, 1077
BtsIMutI CAGTG 7 cut(s) 622, 760, 783, 966, 1077, 1541, 1647
BveI ACCTGC 1 cut(s) 1073
Cac8I GCNNGC 3 cut(s) 115, 232, 329
CciI TCATGA 1 cut(s) 901
CfoI GCGC 2 cut(s) 316, 1390
Cfr13I GGNCC 4 cut(s) 788, 1085, 1405, 1571
ClaI ATCGAT 1 cut(s) 815
CseI GACGC 1 cut(s) 403
Csp6I GTAC 3 cut(s) 907, 1181, 1598
CviAII CATG 3 cut(s) 114, 902, 1468
CviQI GTAC 3 cut(s) 907, 1181, 1598
DdeI CTNAG 4 cut(s) 434, 486, 1190, 1498
DpnI GATC 5 cut(s) 183, 368, 818, 878, 1493
DpnII GATC 5 cut(s) 181, 366, 816, 876, 1491
DriI GACNNNNNGTC 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 519, 1053
Eam1104I CTCTTC 2 cut(s) 112, 1586
Eam1105I GACNNNNNGTC 1 cut(s) 304
EarI CTCTTC 2 cut(s) 112, 1586
EciI GGCGGA 1 cut(s) 376
Ecl136II GAGCTC 1 cut(s) 379
Eco105I TACGTA 1 cut(s) 1180
Eco130I CCWWGG 4 cut(s) 262, 582, 1324, 1439
Eco147I AGGCCT 1 cut(s) 1438
Eco24I GRGCYC 2 cut(s) 381, 626
Eco47I GGWCC 3 cut(s) 788, 1085, 1405
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 2 cut(s) 93, 879
Eco81I CCTNAGG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 842
EcoICRI GAGCTC 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 1606
EcoT14I CCWWGG 4 cut(s) 262, 582, 1324, 1439
EcoT38I GRGCYC 2 cut(s) 381, 626
ErhI CCWWGG 4 cut(s) 262, 582, 1324, 1439
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 3 cut(s) 117, 905, 1471
FaqI GGGAC 2 cut(s) 811, 1537
FatI CATG 3 cut(s) 113, 901, 1467
FauNDI CATATG 1 cut(s) 793
FbaI TGATCA 1 cut(s) 1491
FokI GGATG 5 cut(s) 76, 88, 906, 1349, 1518
FriOI GRGCYC 2 cut(s) 381, 626
FspBI CTAG 1 cut(s) 1455
GlaI GCGC 2 cut(s) 315, 1389
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 6 cut(s) 521, 527, 1055, 1438, 1573, 1606
HapII CCGG 5 cut(s) 627, 652, 978, 1353, 1574
HgaI GACGC 1 cut(s) 403
HhaI GCGC 2 cut(s) 316, 1390
Hin1II CATG 3 cut(s) 117, 905, 1471
Hin6I GCGC 2 cut(s) 314, 1388
HinP1I GCGC 2 cut(s) 314, 1388
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 5 cut(s) 305, 733, 1342, 1356, 1564
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 5 cut(s) 627, 652, 978, 1353, 1574
HphI GGTGA 4 cut(s) 165, 737, 1565, 1682
Hpy166II GTNNAC 4 cut(s) 123, 415, 643, 769
Hpy188III TCNNGA 8 cut(s) 370, 737, 842, 902, 1072, 1088, 1166, 1264
Hpy8I GTNNAC 4 cut(s) 123, 415, 643, 769
Hpy99I CGWCG 3 cut(s) 712, 998, 1591
HpyAV CCTTC 7 cut(s) 538, 1021, 1151, 1324, 1428, 1438, 1711
HpyCH4III ACNGT 9 cut(s) 177, 778, 970, 1228, 1462, 1528, 1545, 1553, 1651
HpyCH4IV ACGT 4 cut(s) 348, 428, 1179, 1418
HpyCH4V TGCA 9 cut(s) 86, 117, 424, 643, 1082, 1149, 1272, 1374, 1471
HpyF10VI GCNNNNNNNGC 6 cut(s) 430, 621, 1037, 1158, 1319, 1634
HpyF3I CTNAG 4 cut(s) 434, 486, 1190, 1498
HpySE526I ACGT 4 cut(s) 348, 428, 1179, 1418
Hsp92II CATG 3 cut(s) 117, 905, 1471
HspAI GCGC 2 cut(s) 314, 1388
Ksp22I TGATCA 1 cut(s) 1491
KspAI GTTAAC 1 cut(s) 769
Kzo9I GATC 5 cut(s) 181, 366, 816, 876, 1491
LmnI GCTCC 6 cut(s) 20, 173, 340, 352, 629, 844
LweI GCATC 5 cut(s) 95, 141, 814, 884, 1284
MaeI CTAG 1 cut(s) 1455
MaeII ACGT 4 cut(s) 348, 428, 1179, 1418
MaeIII GTNAC 5 cut(s) 445, 464, 538, 571, 1211
MalI GATC 5 cut(s) 183, 368, 818, 878, 1493
MbiI CCGCTC 1 cut(s) 438
MboI GATC 5 cut(s) 181, 366, 816, 876, 1491
MboII GAAGA 6 cut(s) 19, 99, 752, 872, 1252, 1603
MfeI CAATTG 1 cut(s) 1377
MhlI GDGCHC 4 cut(s) 357, 381, 626, 645
MluCI AATT 8 cut(s) 249, 647, 1273, 1377, 1423, 1642, 1657, 1720
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 1483, 1491, 1510
MseI TTAA 5 cut(s) 252, 675, 768, 960, 1421
MspA1I CMGCKG 1 cut(s) 1403
MspI CCGG 5 cut(s) 627, 652, 978, 1353, 1574
MspR9I CCNGG 3 cut(s) 653, 1575, 1608
MunI CAATTG 1 cut(s) 1377
Mva1269I GAATGC 3 cut(s) 577, 759, 1630
MvaI CCWGG 1 cut(s) 1608
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 6 cut(s) 430, 621, 1037, 1158, 1319, 1634
NciI CCSGG 2 cut(s) 653, 1575
NdeI CATATG 1 cut(s) 793
NdeII GATC 5 cut(s) 181, 366, 816, 876, 1491
NlaIII CATG 3 cut(s) 117, 905, 1471
NlaIV GGNNCC 3 cut(s) 625, 631, 789
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 3 cut(s) 445, 571, 1211
NspI RCATGY 1 cut(s) 117
NspV TTCGAA 1 cut(s) 1155
PaeI GCATGC 1 cut(s) 117
PagI TCATGA 1 cut(s) 901
PceI AGGCCT 1 cut(s) 1438
PctI GAATGC 3 cut(s) 577, 759, 1630
PfeI GAWTC 4 cut(s) 733, 1342, 1356, 1564
Ple19I CGATCG 1 cut(s) 819
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Ppu21I YACGTR 1 cut(s) 1180
PshBI ATTAAT 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 1606
PspGI CCWGG 1 cut(s) 1606
PspN4I GGNNCC 3 cut(s) 625, 631, 789
PspPI GGNCC 4 cut(s) 788, 1085, 1405, 1571
PstI CTGCAG 1 cut(s) 1084
PvuI CGATCG 1 cut(s) 819
RsaI GTAC 3 cut(s) 908, 1182, 1599
RsaNI GTAC 3 cut(s) 907, 1181, 1598
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 5 cut(s) 252, 675, 768, 960, 1421
Sau3AI GATC 5 cut(s) 181, 366, 816, 876, 1491
Sau96I GGNCC 4 cut(s) 788, 1085, 1405, 1571
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 653, 1575, 1608
SduI GDGCHC 4 cut(s) 357, 381, 626, 645
SfaNI GCATC 5 cut(s) 95, 141, 814, 884, 1284
SfcI CTRYAG 4 cut(s) 187, 550, 1080, 1227
SfuI TTCGAA 1 cut(s) 1155
SinI GGWCC 3 cut(s) 788, 1085, 1405
SmlI CTYRAG 2 cut(s) 380, 1632
SmoI CTYRAG 2 cut(s) 380, 1632
SnaBI TACGTA 1 cut(s) 1180
SphI GCATGC 1 cut(s) 117
Sse9I AATT 8 cut(s) 249, 647, 1273, 1377, 1423, 1642, 1657, 1720
SseBI AGGCCT 1 cut(s) 1438
SsiI CCGC 5 cut(s) 387, 438, 1040, 1103, 1403
SspMI CTAG 1 cut(s) 1455
SstI GAGCTC 1 cut(s) 381
StuI AGGCCT 1 cut(s) 1438
StyD4I CCNGG 3 cut(s) 651, 1573, 1606
StyI CCWWGG 4 cut(s) 262, 582, 1324, 1439
TaaI ACNGT 9 cut(s) 177, 778, 970, 1228, 1462, 1528, 1545, 1553, 1651
TaiI ACGT 4 cut(s) 351, 431, 1182, 1421
TaqI TCGA 7 cut(s) 149, 371, 707, 815, 996, 1155, 1285
TasI AATT 8 cut(s) 249, 647, 1273, 1377, 1423, 1642, 1657, 1720
TatI WGTACW 2 cut(s) 906, 1597
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 4 cut(s) 733, 1342, 1356, 1564
Tru1I TTAA 5 cut(s) 252, 675, 768, 960, 1421
Tru9I TTAA 5 cut(s) 252, 675, 768, 960, 1421
TscAI CASTG 7 cut(s) 622, 760, 783, 973, 1084, 1548, 1654
TseFI GTSAC 3 cut(s) 445, 571, 1211
Tsp45I GTSAC 3 cut(s) 445, 571, 1211
TspDTI ATGAA 4 cut(s) 280, 661, 1225, 1637
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 7 cut(s) 622, 760, 783, 973, 1084, 1548, 1654
VneI GTGCAC 1 cut(s) 641
VpaK11BI GGWCC 3 cut(s) 788, 1085, 1405
VspI ATTAAT 1 cut(s) 960
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 3 cut(s) 647, 1423, 1642
XceI RCATGY 1 cut(s) 117
XspI CTAG 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.