pycom02g22790

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011631
Physical Location & Seq
Reverse (-)
20966301 .. 20967257
957 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22790.1

Sequence Viewer

Length: 957 bp
ATGTACACGCGCATCCCTGCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATAAAAGAGATCCCCAACACACATTTTCATACTCAACTCAACCTAGAGCCCCCCGAAAAACAATACATTTTCACACTCAAAATGCCACGGCTACCCTCCTCTCCTTTCTTCCTCCTCCTCCTTTCTCTATCCCTCTTTTCACTACTTCTGCTAACCTCTGGTGCTGCTGCATCATCCCCCTCATCAGCCTCTTCAGCATGCAAGTCCACGCTCTACCCAAAGCTCTGCCGTTCGATGCTCTCCACCATTCGCTCCTCACCGTCCGATCCCTACAACTACGGCAAGTTCTCCATCAAACAATGCCTGAAACAGGCACGCAAAACCTCCACAACAATCAACCACTTCCTCGGAAAAATCAAGCACAAGTCGTTTTTCATAAACGACAGCGAGTCGAACGCGCTGGAGGACTGCCAGCAGTTCTCGGAGCTGAACGTAGAGCACTTGGAGGCGATCTCGATAGAGCTCAAGTCTGCCGAGACGCTGAATGAGGAACTGGTGGACAAGGTACAGACATTGCTAAGCGGCATTGTGACCAACCAGCAGACTTGTTACGATGAGCTTGTGGTGTCTAAGAGCAGCGTTTTGGGAAACACCACGGCTTTGGCCGTGGCCTTCTCTAATGTTACGCAGCTCTATAGTGTGTCGCTTGGGTTGGTCACGCAATCCTTGGCTCGTAATCTCAAAAAGCATAAGAGAGGCAGTGAGGGCTCCGGCACAAAAAATGTGCACAAATTCCGGGAACCACTTGATATTAAGGTAAAGCTTTTGAGTTCATTTTTCTTGACATTTCTGGTTTTTTTTTTTTTTTTTTTTTTTTTCACAAAAATGTTATTTTACCATTCATTTGCACTATTTTTCTAA

Protein Analysis

319

Amino Acids

35.75

Weight (kDa)

9.3

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 95 - 249 5.8e-22 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011631)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10720 AT3G10720 AT5G04970
fragaria_vesca FvH4_7g04220
malus_domestica MD02G1266500.v1.1 MD07G1053200.v1.1
prunus_persica Prupe.2G058200_v2.0.a1
pyrus_communis pycom02g22780 pycom02g22790
rosa_chinensis RchiOBHm_Chr1g0330991
rosa_laevigata RLG00000029762
rosa_multiflora Rmu_sc0002001.1_g000004
rosa_roxburghii Rroxscaffold_4G00319570
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113000 Rh1BG087000 Rh1CG109900 Rh1DG118700
rosa_wichuraiana Rw1G009210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 10, 494
AciI CCGC 1 cut(s) 618
AclWI GGATC 2 cut(s) 100, 356
AcoI YGGCCR 1 cut(s) 699
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 2 cut(s) 5, 603
AfiI CCNNNNNNNGG 1 cut(s) 406
AhdI GACNNNNNGTC 1 cut(s) 484
AluBI AGCT 6 cut(s) 319, 523, 559, 655, 727, 859
AluI AGCT 6 cut(s) 319, 523, 559, 655, 727, 859
Alw21I GWGCWC 3 cut(s) 537, 561, 825
Alw26I GTCTC 1 cut(s) 566
Alw44I GTGCAC 1 cut(s) 821
AlwI GGATC 2 cut(s) 100, 356
AoxI GGCC 2 cut(s) 699, 705
ApaLI GTGCAC 1 cut(s) 821
ApeKI GCWGC 4 cut(s) 260, 263, 672, 724
ApoI RAATTY 1 cut(s) 827
AspLEI GCGC 2 cut(s) 12, 496
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 825
BanII GRGCYC 3 cut(s) 147, 561, 806
Bbv12I GWGCWC 3 cut(s) 537, 561, 825
BbvI GCAGC 4 cut(s) 247, 250, 684, 736
BccI CCATC 1 cut(s) 395
BceAI ACGGC 5 cut(s) 200, 309, 391, 686, 708
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 730
BisI GCNGC 5 cut(s) 261, 264, 619, 673, 725
BlpI GCTNAGC 1 cut(s) 614
BlsI GCNGC 5 cut(s) 262, 265, 620, 674, 726
Bme1390I CCNGG 1 cut(s) 833
BmeRI GACNNNNNGTC 1 cut(s) 484
BmiI GGNNCC 2 cut(s) 805, 837
BmrFI CCNGG 1 cut(s) 833
BmsI GCATC 4 cut(s) 21, 29, 275, 321
BplI GAGNNNNNCTC 2 cut(s) 533, 565
BpmI CTGGAG 1 cut(s) 518
Bpu1102I GCTNAGC 1 cut(s) 614
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 833
BsaJI CCNNGG 5 cut(s) 182, 442, 690, 702, 762
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 594
Bse3DI GCAATG 1 cut(s) 608
BseDI CCNNGG 5 cut(s) 182, 442, 690, 702, 762
BseGI GGATG 2 cut(s) 12, 269
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMI GCAATG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 594
BseRI GAGGAG 4 cut(s) 184, 200, 203, 340
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 4 cut(s) 247, 250, 684, 736
Bsh1236I CGCG 2 cut(s) 10, 494
BshFI GGCC 2 cut(s) 701, 707
BsiHKAI GWGCWC 3 cut(s) 537, 561, 825
BsiSI CCGG 2 cut(s) 807, 832
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 566
BsmBI CGTCTC 1 cut(s) 566
BsnI GGCC 2 cut(s) 701, 707
Bsp1286I GDGCHC 5 cut(s) 147, 537, 561, 806, 825
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 105, 361, 546
Bsp1720I GCTNAGC 1 cut(s) 614
BspACI CCGC 1 cut(s) 618
BspANI GGCC 2 cut(s) 701, 707
BspFNI CGCG 2 cut(s) 10, 494
BspLI GGNNCC 2 cut(s) 805, 837
BspPI GGATC 2 cut(s) 100, 356
BsrDI GCAATG 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 594
BssECI CCNNGG 5 cut(s) 182, 442, 690, 702, 762
BssMI GATC 3 cut(s) 105, 361, 546
BssT1I CCWWGG 1 cut(s) 762
Bst4CI ACNGT 1 cut(s) 357
Bst6I CTCTTC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 295, 412, 509
BstDEI CTNAG 2 cut(s) 614, 666
BstDSI CCRYGG 3 cut(s) 182, 690, 702
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 12, 269
BstFNI CGCG 2 cut(s) 10, 494
BstHHI GCGC 2 cut(s) 12, 496
BstKTI GATC 3 cut(s) 108, 364, 549
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 3 cut(s) 105, 361, 546
BstMWI GCNNNNNNNGC 2 cut(s) 290, 801
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 831
BstSFI CTRYAG 1 cut(s) 730
BstSLI GKGCMC 1 cut(s) 825
BstUI CGCG 2 cut(s) 10, 494
BstV1I GCAGC 4 cut(s) 247, 250, 684, 736
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 2 cut(s) 701, 707
BtgI CCRYGG 3 cut(s) 182, 690, 702
BtsCI GGATG 2 cut(s) 12, 269
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 1 cut(s) 802
Cac8I GCNNGC 3 cut(s) 295, 412, 509
CfoI GCGC 2 cut(s) 12, 496
CseI GACGC 1 cut(s) 583
Csp6I GTAC 2 cut(s) 4, 602
CviAII CATG 1 cut(s) 294
CviQI GTAC 2 cut(s) 4, 602
DdeI CTNAG 2 cut(s) 614, 666
DpnI GATC 3 cut(s) 107, 363, 548
DpnII GATC 3 cut(s) 105, 361, 546
DriI GACNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 1 cut(s) 292
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 3 cut(s) 147, 561, 806
Eco53kI GAGCTC 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 559
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 3 cut(s) 147, 561, 806
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 1 cut(s) 297
FatI CATG 1 cut(s) 293
Fnu4HI GCNGC 5 cut(s) 261, 264, 619, 673, 725
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 3 cut(s) 147, 561, 806
Fsp4HI GCNGC 5 cut(s) 261, 264, 619, 673, 725
FspBI CTAG 1 cut(s) 140
GlaI GCGC 2 cut(s) 11, 495
GluI GCNGC 5 cut(s) 261, 264, 619, 673, 725
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 701, 707
HapII CCGG 2 cut(s) 807, 832
HgaI GACGC 1 cut(s) 583
HhaI GCGC 2 cut(s) 12, 496
Hin1II CATG 1 cut(s) 297
Hin6I GCGC 2 cut(s) 10, 494
HinP1I GCGC 2 cut(s) 10, 494
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 1 cut(s) 485
HpaII CCGG 2 cut(s) 807, 832
HphI GGTGA 1 cut(s) 345
Hpy166II GTNNAC 4 cut(s) 6, 303, 595, 823
Hpy188I TCNGA 3 cut(s) 361, 446, 520
Hpy188III TCNNGA 2 cut(s) 550, 877
Hpy8I GTNNAC 4 cut(s) 6, 303, 595, 823
HpyAV CCTTC 1 cut(s) 718
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 5 cut(s) 20, 266, 297, 823, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 801
HpyF3I CTNAG 2 cut(s) 614, 666
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 1 cut(s) 297
HspAI GCGC 2 cut(s) 10, 494
Kzo9I GATC 3 cut(s) 105, 361, 546
LmnI GCTCC 3 cut(s) 353, 520, 809
Lsp1109I GCAGC 4 cut(s) 247, 250, 684, 736
LweI GCATC 4 cut(s) 21, 29, 275, 321
MaeI CTAG 1 cut(s) 140
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 4 cut(s) 625, 644, 718, 751
MalI GATC 3 cut(s) 107, 363, 548
MboI GATC 3 cut(s) 105, 361, 546
MboII GAAGA 2 cut(s) 196, 279
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 5 cut(s) 147, 537, 561, 806, 825
MluCI AATT 1 cut(s) 827
MlyI GAGTC 1 cut(s) 494
MseI TTAA 1 cut(s) 849
MslI CAYNNNNRTG 1 cut(s) 920
MspI CCGG 2 cut(s) 807, 832
MspR9I CCNGG 1 cut(s) 833
MvnI CGCG 2 cut(s) 10, 494
MwoI GCNNNNNNNGC 2 cut(s) 290, 801
NciI CCSGG 1 cut(s) 833
NdeII GATC 3 cut(s) 105, 361, 546
NlaIII CATG 1 cut(s) 297
NlaIV GGNNCC 2 cut(s) 805, 837
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 2 cut(s) 625, 751
NspI RCATGY 1 cut(s) 297
PaeI GCATGC 1 cut(s) 297
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 5 cut(s) 262, 265, 620, 674, 726
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
Psp124BI GAGCTC 1 cut(s) 561
PspN4I GGNNCC 2 cut(s) 805, 837
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 2 cut(s) 5, 603
RsaNI GTAC 2 cut(s) 4, 602
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 1 cut(s) 561
SaqAI TTAA 1 cut(s) 849
SatI GCNGC 5 cut(s) 261, 264, 619, 673, 725
Sau3AI GATC 3 cut(s) 105, 361, 546
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 5 cut(s) 147, 537, 561, 806, 825
SfaNI GCATC 4 cut(s) 21, 29, 275, 321
SfcI CTRYAG 1 cut(s) 730
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
SphI GCATGC 1 cut(s) 297
Sse9I AATT 1 cut(s) 827
SsiI CCGC 1 cut(s) 618
SspMI CTAG 1 cut(s) 140
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 831
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 357
TaiI ACGT 1 cut(s) 531
TaqI TCGA 3 cut(s) 329, 488, 551
TasI AATT 1 cut(s) 827
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 621
Tru1I TTAA 1 cut(s) 849
Tru9I TTAA 1 cut(s) 849
TscAI CASTG 1 cut(s) 802
TseFI GTSAC 2 cut(s) 625, 751
TseI GCWGC 4 cut(s) 260, 263, 672, 724
Tsp45I GTSAC 2 cut(s) 625, 751
TspDTI ATGAA 4 cut(s) 113, 460, 858, 927
TspRI CASTG 1 cut(s) 802
VneI GTGCAC 1 cut(s) 821
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 297
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.