Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009334

Physical Location & Seq

Genomic Coordinates Reverse (-)

5639704 .. 5642774

Gene Structure 3071 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G16565.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 762 bp

ATGGATTTTCCTCCGACGAAGCTTGATTATCATGTAGACATGTTCAATCTTCAGTCTCATTCCAGATTTCTCTCCTTATTTAAGGCGCAAGATGGACGCATAGCTCTAATACTAGAATCAACGGTGTTTCATCCACAAGGTGGTGGCCAGCCGTCAGACACCGGTTTAATTGTTTTCTCCGGTTCGGATTTGAAATTTTCCGTTCAAGATGTTCGATCGAAAGACGGAATTGTTCTCCATTACGGAGTTTTCGAAGGTTCGAATCCTGAAAGTGGAATTGATAGTGAGAAAGGGAAAGAAGTTTACTTAACTGTTGATGAATCAAGGCGTAAACTCAATTCCAGGTTGCACTCAGCTGGACACTTGCTAGATATGTGTATGCAGAAAGTTGGGTTAGGACATTTGGAGCCTGGAAAAGGGTACCATTTTCCTGACGGTCCTTTTGTGGAATACAAAGGAAGCGTCCCACAGGAGGAGTTTCAGGTGAAGCAGAAAGAGTTGGAGGCAGAAGCTAACGAACTGATATCCAAAGGAGGAAAGGTTTATGCTGCTATATTGCCCTATGAAGAGGCATCTGTGCTCTGTGGTGGCAGTCTTCCTGATTATATTTCCAAGGGCAGCACTCCCCGGATCATAAAATTAGGTGACAGCCCCGGGTGTCCATGTGGTGGAACCCATGTCTCCAATTTATCCGATATCATAAGCATGAAGATGAGAACAAAGAAAGGAATGACGAAAGTTTTCTACACCATTGCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

tRNA-synt_2c tRNA_SAD

Protein Analysis

253

Amino Acids

27.68

Weight (kDa)

6.31

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	33 - 107	7e-09	tRNA synthetases class II (A)
tRNA_SAD	PF07973	210 - 245	7.4e-06	Threonyl and Alanyl tRNA synthetase second additional domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009334)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16565 AT3G16565 AT3G16565 AT3G16565 AT3G16565 AT3G16565
fragaria_vesca	FvH4_4g17960
malus_domestica	MD13G1169000.v1.1 MD16G1169600.v1.1
prunus_persica	Prupe.1G137400_v2.0.a1
pyrus_communis	pycom13g14460
rosa_chinensis	RchiOBHm_Chr4g0422131
rosa_laevigata	RLG00000007632
rosa_multiflora	Rmu_sc0004902.1_g000006 Rmu_sc0036261.1_g000001
rosa_roxburghii	Rroxscaffold_5G00364210
rosa_rugosa	Rorug04G0177600
rosa_samantha	Rh4AG237100 Rh4BG239900 Rh4CG251800 Rh4DG236000
rosa_wichuraiana	Rw4G020510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	420
AccB1I	GGYRCC	1 cut(s)	420
AccB7I	CCANNNNNTGG	2 cut(s)	140, 668
AccI	GTMKAC	1 cut(s)	36
AclWI	GGATC	1 cut(s)	638
AcoI	YGGCCR	1 cut(s)	145
AcsI	RAATTY	1 cut(s)	194
AcuI	CTGAAG	1 cut(s)	35
AdeI	CACNNNGTG	1 cut(s)	140
AfaI	GTAC	1 cut(s)	422
AfiI	CCNNNNNNNGG	5 cut(s)	140, 272, 416, 472, 668
AflIII	ACRYGT	1 cut(s)	39
AgeI	ACCGGT	1 cut(s)	161
AgsI	TTSAA	3 cut(s)	46, 193, 206
AjnI	CCWGG	2 cut(s)	341, 409
AluBI	AGCT	4 cut(s)	22, 104, 356, 512
AluI	AGCT	4 cut(s)	22, 104, 356, 512
Alw21I	GWGCWC	1 cut(s)	582
Alw26I	GTCTC	2 cut(s)	60, 685
AlwI	GGATC	1 cut(s)	638
Ama87I	CYCGRG	1 cut(s)	653
AoxI	GGCC	1 cut(s)	145
ApeKI	GCWGC	2 cut(s)	548, 618
ApoI	RAATTY	1 cut(s)	194
ArsI	GACNNNNNNTTYG	4 cut(s)	425, 447, 457, 479
AsiGI	ACCGGT	1 cut(s)	161
Asp700I	GAANNNNTTC	1 cut(s)	740
Asp718I	GGTACC	1 cut(s)	420
AspLEI	GCGC	1 cut(s)	88
AspS9I	GGNCC	1 cut(s)	437
AsuC2I	CCSGG	3 cut(s)	628, 654, 655
AsuHPI	GGTGA	2 cut(s)	496, 656
AsuII	TTCGAA	2 cut(s)	252, 260
AvaI	CYCGRG	1 cut(s)	653
AvaII	GGWCC	1 cut(s)	437
BalI	TGGCCA	1 cut(s)	147
BanI	GGYRCC	1 cut(s)	420
BbsI	GAAGAC	1 cut(s)	587
Bbv12I	GWGCWC	1 cut(s)	582
BbvI	GCAGC	2 cut(s)	535, 630
BccI	CCATC	1 cut(s)	86
BceAI	ACGGC	1 cut(s)	136
BciT130I	CCWGG	2 cut(s)	343, 411
BcnI	CCSGG	3 cut(s)	628, 654, 655
BcoDI	GTCTC	2 cut(s)	60, 685
BfaI	CTAG	2 cut(s)	113, 368
BisI	GCNGC	2 cut(s)	549, 619
BlsI	GCNGC	2 cut(s)	550, 620
Bme1390I	CCNGG	5 cut(s)	343, 411, 628, 654, 655
Bme18I	GGWCC	1 cut(s)	437
BmeT110I	CYCGRG	1 cut(s)	653
BmgT120I	GGNCC	1 cut(s)	437
BmiI	GGNNCC	3 cut(s)	408, 422, 673
BmrFI	CCNGG	5 cut(s)	343, 411, 628, 654, 655
BmsI	GCATC	1 cut(s)	581
BpiI	GAAGAC	1 cut(s)	587
Bpu14I	TTCGAA	2 cut(s)	252, 260
BpuMI	CCSGG	3 cut(s)	628, 654, 655
BsaJI	CCNNGG	4 cut(s)	612, 626, 652, 653
BsaWI	WCCGGW	2 cut(s)	161, 179
BsaXI	ACNNNNNCTCC	2 cut(s)	525, 555
Bsc4I	CCNNNNNNNGG	5 cut(s)	140, 272, 416, 472, 668
Bse118I	RCCGGY	1 cut(s)	161
Bse3DI	GCAATG	1 cut(s)	750
BseBI	CCWGG	2 cut(s)	343, 411
BseDI	CCNNGG	4 cut(s)	612, 626, 652, 653
BseGI	GGATG	1 cut(s)	130
BseLI	CCNNNNNNNGG	5 cut(s)	140, 272, 416, 472, 668
BseMI	GCAATG	1 cut(s)	750
BseMII	CTCAG	1 cut(s)	366
BseRI	GAGGAG	1 cut(s)	488
BseXI	GCAGC	2 cut(s)	535, 630
Bsh1285I	CGRYCG	1 cut(s)	218
BshFI	GGCC	1 cut(s)	147
BshNI	GGYRCC	1 cut(s)	420
BshTI	ACCGGT	1 cut(s)	161
BsiEI	CGRYCG	1 cut(s)	218
BsiHKAI	GWGCWC	1 cut(s)	582
BsiHKCI	CYCGRG	1 cut(s)	653
BsiSI	CCGG	4 cut(s)	162, 180, 628, 654
BslFI	GGGAC	1 cut(s)	449
BslI	CCNNNNNNNGG	5 cut(s)	140, 272, 416, 472, 668
BsmAI	GTCTC	2 cut(s)	60, 685
BsmFI	GGGAC	1 cut(s)	449
BsnI	GGCC	1 cut(s)	147
BsoBI	CYCGRG	1 cut(s)	653
Bsp119I	TTCGAA	2 cut(s)	252, 260
Bsp1286I	GDGCHC	1 cut(s)	582
Bsp143I	GATC	2 cut(s)	215, 630
BspANI	GGCC	1 cut(s)	147
BspCNI	CTCAG	1 cut(s)	365
BspLI	GGNNCC	3 cut(s)	408, 422, 673
BspPI	GGATC	1 cut(s)	638
BspT104I	TTCGAA	2 cut(s)	252, 260
BspT107I	GGYRCC	1 cut(s)	420
BsrDI	GCAATG	1 cut(s)	750
BsrFI	RCCGGY	1 cut(s)	161
BssAI	RCCGGY	1 cut(s)	161
BssECI	CCNNGG	4 cut(s)	612, 626, 652, 653
BssMI	GATC	2 cut(s)	215, 630
BssT1I	CCWWGG	1 cut(s)	612
Bst2UI	CCWGG	2 cut(s)	343, 411
Bst4CI	ACNGT	3 cut(s)	124, 313, 437
Bst6I	CTCTTC	1 cut(s)	561
BstBI	TTCGAA	2 cut(s)	252, 260
BstC8I	GCNNGC	1 cut(s)	149
BstDEI	CTNAG	1 cut(s)	352
BstENI	CCTNNNNNAGG	1 cut(s)	414
BstF5I	GGATG	1 cut(s)	130
BstHHI	GCGC	1 cut(s)	88
BstKTI	GATC	2 cut(s)	218, 633
BstMAI	GTCTC	2 cut(s)	60, 685
BstMBI	GATC	2 cut(s)	215, 630
BstMCI	CGRYCG	1 cut(s)	218
BstNI	CCWGG	2 cut(s)	343, 411
BstNSI	RCATGY	1 cut(s)	43
BstSCI	CCNGG	5 cut(s)	341, 409, 626, 652, 653
BstV1I	GCAGC	2 cut(s)	535, 630
BstV2I	GAAGAC	1 cut(s)	587
BsuRI	GGCC	1 cut(s)	147
BtsCI	GGATG	1 cut(s)	130
Cac8I	GCNNGC	1 cut(s)	149
CfoI	GCGC	1 cut(s)	88
Cfr10I	RCCGGY	1 cut(s)	161
Cfr13I	GGNCC	1 cut(s)	437
Cfr9I	CCCGGG	1 cut(s)	653
CseI	GACGC	2 cut(s)	105, 451
Csp6I	GTAC	1 cut(s)	421
CspAI	ACCGGT	1 cut(s)	161
CviAII	CATG	5 cut(s)	32, 40, 663, 677, 706
CviJI	RGCY	8 cut(s)	22, 104, 147, 151, 356, 409, 512, 651
CviKI_1	RGCY	8 cut(s)	22, 104, 147, 151, 356, 409, 512, 651
CviQI	GTAC	1 cut(s)	421
DdeI	CTNAG	1 cut(s)	352
DpnI	GATC	2 cut(s)	217, 632
DpnII	GATC	2 cut(s)	215, 630
DraIII	CACNNNGTG	1 cut(s)	140
EaeI	YGGCCR	1 cut(s)	145
Eam1104I	CTCTTC	1 cut(s)	561
EarI	CTCTTC	1 cut(s)	561
Eco130I	CCWWGG	1 cut(s)	612
Eco32I	GATATC	2 cut(s)	525, 697
Eco47I	GGWCC	1 cut(s)	437
Eco57I	CTGAAG	1 cut(s)	35
Eco88I	CYCGRG	1 cut(s)	653
EcoNI	CCTNNNNNAGG	1 cut(s)	414
EcoRII	CCWGG	2 cut(s)	341, 409
EcoRV	GATATC	2 cut(s)	525, 697
EcoT14I	CCWWGG	1 cut(s)	612
ErhI	CCWWGG	1 cut(s)	612
FaeI	CATG	5 cut(s)	35, 43, 666, 680, 709
FaqI	GGGAC	1 cut(s)	449
FatI	CATG	5 cut(s)	31, 39, 662, 676, 705
FblI	GTMKAC	1 cut(s)	36
Fnu4HI	GCNGC	2 cut(s)	549, 619
FokI	GGATG	1 cut(s)	117
Fsp4HI	GCNGC	2 cut(s)	549, 619
FspBI	CTAG	2 cut(s)	113, 368
GlaI	GCGC	1 cut(s)	87
GluI	GCNGC	2 cut(s)	549, 619
HaeIII	GGCC	1 cut(s)	147
HapII	CCGG	4 cut(s)	162, 180, 628, 654
HgaI	GACGC	2 cut(s)	105, 451
HhaI	GCGC	1 cut(s)	88
Hin1II	CATG	5 cut(s)	35, 43, 666, 680, 709
Hin6I	GCGC	1 cut(s)	86
HinP1I	GCGC	1 cut(s)	86
HindIII	AAGCTT	1 cut(s)	20
HinfI	GANTC	3 cut(s)	116, 262, 320
HpaII	CCGG	4 cut(s)	162, 180, 628, 654
HphI	GGTGA	2 cut(s)	496, 656
Hpy166II	GTNNAC	3 cut(s)	37, 304, 332
Hpy188I	TCNGA	4 cut(s)	15, 157, 187, 694
Hpy188III	TCNNGA	6 cut(s)	63, 206, 266, 431, 599, 759
Hpy8I	GTNNAC	3 cut(s)	37, 304, 332
Hpy99I	CGWCG	1 cut(s)	19
HpyAV	CCTTC	1 cut(s)	248
HpyCH4III	ACNGT	3 cut(s)	124, 313, 437
HpyCH4V	TGCA	3 cut(s)	349, 382, 755
HpyF3I	CTNAG	1 cut(s)	352
Hsp92II	CATG	5 cut(s)	35, 43, 666, 680, 709
HspAI	GCGC	1 cut(s)	86
KpnI	GGTACC	1 cut(s)	424
Kzo9I	GATC	2 cut(s)	215, 630
LmnI	GCTCC	1 cut(s)	406
Lsp1109I	GCAGC	2 cut(s)	535, 630
LweI	GCATC	1 cut(s)	581
MaeI	CTAG	2 cut(s)	113, 368
MaeIII	GTNAC	1 cut(s)	644
MalI	GATC	2 cut(s)	217, 632
MboI	GATC	2 cut(s)	215, 630
MboII	GAAGA	4 cut(s)	41, 578, 587, 721
MhlI	GDGCHC	1 cut(s)	582
MlsI	TGGCCA	1 cut(s)	147
MluCI	AATT	7 cut(s)	168, 194, 228, 276, 337, 638, 685
MluNI	TGGCCA	1 cut(s)	147
MmeI	TCCRAC	2 cut(s)	38, 480
MnlI	CCTC	5 cut(s)	21, 466, 496, 527, 562
Mox20I	TGGCCA	1 cut(s)	147
MroXI	GAANNNNTTC	1 cut(s)	740
MscI	TGGCCA	1 cut(s)	147
MseI	TTAA	3 cut(s)	81, 167, 308
MslI	CAYNNNNRTG	2 cut(s)	704, 710
Msp20I	TGGCCA	1 cut(s)	147
MspA1I	CMGCKG	1 cut(s)	356
MspI	CCGG	4 cut(s)	162, 180, 628, 654
MspR9I	CCNGG	5 cut(s)	343, 411, 628, 654, 655
MvaI	CCWGG	2 cut(s)	343, 411
NciI	CCSGG	3 cut(s)	628, 654, 655
NdeII	GATC	2 cut(s)	215, 630
NlaIII	CATG	5 cut(s)	35, 43, 666, 680, 709
NlaIV	GGNNCC	3 cut(s)	408, 422, 673
NmuCI	GTSAC	1 cut(s)	644
NspI	RCATGY	1 cut(s)	43
NspV	TTCGAA	2 cut(s)	252, 260
PciI	ACATGT	1 cut(s)	39
PcsI	WCGNNNNNNNCGW	1 cut(s)	249
PdmI	GAANNNNTTC	1 cut(s)	740
PfeI	GAWTC	3 cut(s)	116, 262, 320
PflMI	CCANNNNNTGG	2 cut(s)	140, 668
PinAI	ACCGGT	1 cut(s)	161
PkrI	GCNGC	2 cut(s)	550, 620
Ple19I	CGATCG	1 cut(s)	218
PscI	ACATGT	1 cut(s)	39
Psp6I	CCWGG	2 cut(s)	341, 409
PspGI	CCWGG	2 cut(s)	341, 409
PspN4I	GGNNCC	3 cut(s)	408, 422, 673
PspPI	GGNCC	1 cut(s)	437
PvuI	CGATCG	1 cut(s)	218
PvuII	CAGCTG	1 cut(s)	356
RsaI	GTAC	1 cut(s)	422
RsaNI	GTAC	1 cut(s)	421
RseI	CAYNNNNRTG	2 cut(s)	704, 710
SaqAI	TTAA	3 cut(s)	81, 167, 308
SatI	GCNGC	2 cut(s)	549, 619
Sau3AI	GATC	2 cut(s)	215, 630
Sau96I	GGNCC	1 cut(s)	437
ScrFI	CCNGG	5 cut(s)	343, 411, 628, 654, 655
SduI	GDGCHC	1 cut(s)	582
SfaNI	GCATC	1 cut(s)	581
SfuI	TTCGAA	2 cut(s)	252, 260
SinI	GGWCC	1 cut(s)	437
SmaI	CCCGGG	1 cut(s)	655
SmiMI	CAYNNNNRTG	2 cut(s)	704, 710
Sse9I	AATT	7 cut(s)	168, 194, 228, 276, 337, 638, 685
SspMI	CTAG	2 cut(s)	113, 368
StyD4I	CCNGG	5 cut(s)	341, 409, 626, 652, 653
StyI	CCWWGG	1 cut(s)	612
TaaI	ACNGT	3 cut(s)	124, 313, 437
TaqI	TCGA	4 cut(s)	214, 218, 252, 260
TasI	AATT	7 cut(s)	168, 194, 228, 276, 337, 638, 685
TfiI	GAWTC	3 cut(s)	116, 262, 320
Tru1I	TTAA	3 cut(s)	81, 167, 308
Tru9I	TTAA	3 cut(s)	81, 167, 308
TseFI	GTSAC	1 cut(s)	644
TseI	GCWGC	2 cut(s)	548, 618
Tsp45I	GTSAC	1 cut(s)	644
TspDTI	ATGAA	4 cut(s)	119, 333, 579, 722
TspGWI	ACGGA	3 cut(s)	190, 240, 258
TspMI	CCCGGG	1 cut(s)	653
Van91I	CCANNNNNTGG	2 cut(s)	140, 668
VpaK11BI	GGWCC	1 cut(s)	437
XagI	CCTNNNNNAGG	1 cut(s)	414
XapI	RAATTY	1 cut(s)	194
XceI	RCATGY	1 cut(s)	43
XmaI	CCCGGG	1 cut(s)	653
XmiI	GTMKAC	1 cut(s)	36
XmnI	GAANNNNTTC	1 cut(s)	740
XspI	CTAG	2 cut(s)	113, 368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.