Rw4G020510

tRNA synthetases class II (A)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009334
Physical Location & Seq
Reverse (-)
44520513 .. 44523055
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020510.1

Sequence Viewer

Length: 774 bp
ATGGGCACGATCATCCCCACGCAGCTTGATTACTACGAAGACATGTGGAAGCTTGAATCCGATGCCACACTGGTAGCATTGGTCAAAGGTGAAGATGGACGGACGGCTTTGGTGCTGGACCGGACGGTGTTCCATCCGCAAGGCGGCGGTCAACCGGCGGACACAGGCTTCGTCGCCGTCGCCGGCTCGGAGGTCAAGTTCGTCGTGCAAGATGTGCGGTCCAAGGACGGTGTAGTTTACCACTACGGTTTGGTTGAGAATTGCGGTGAGGGATGGGAAGCCGAGTTTGAGAAAGGGAGAGAGGTGGTGTTGCATGTTGATGAGTCTAGGCGCAAGCTTAATTCTAGGCTTCACTCAGCTGGGCATTTGCTAGATGTGTGTATGAAGAATGTGGGATTGGGTGATTTAAAGCCAGGCAAAGCCTACCATTTCCCTCAAGGGCCATATGTGGAATATATAGGCATAGTTCCACAGAAAGATGCGCAAAGCAAGCAGAAAGAGTTGGAGTTAGAAGCTAATGCATTGATATCGAGAGGAGGGAAGGTCTTTGTTGCATCAGTACCATATGAAGAAGCATGTAAGCTGTGTGGTGAATGTCTTCCCGATTACATTCCAAAGGACAGCACTCCTCGTATTGTGAAGATGGGGACCAATCCGGGCTGCCCGTGTGGTGGTACTCATGTTTCTGATGTTTCAGAGATCATCAGTGTTACAGTTTCTCAAATACGGACCAAAAAAGGATTCACGAAGGTCTTTTACAATGTTGGTACCTAA

Protein Analysis

257

Amino Acids

28.06

Weight (kDa)

5.84

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2c PF01411 33 - 109 1.6e-10 tRNA synthetases class II (A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 483
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 767
AciI CCGC 6 cut(s) 137, 144, 147, 158, 217, 264
AfaI GTAC 3 cut(s) 561, 676, 769
AfiI CCNNNNNNNGG 2 cut(s) 143, 671
AflIII ACRYGT 1 cut(s) 42
AgsI TTSAA 1 cut(s) 56
AjnI CCWGG 1 cut(s) 412
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 412, 725, 757
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 6 cut(s) 25, 52, 337, 359, 515, 583
AluI AGCT 6 cut(s) 25, 52, 337, 359, 515, 583
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 22, 660
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
Asp700I GAANNNNTTC 1 cut(s) 597
Asp718I GGTACC 1 cut(s) 767
AspLEI GCGC 2 cut(s) 333, 484
AspS9I GGNCC 5 cut(s) 118, 219, 440, 648, 729
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 4 cut(s) 101, 278, 413, 602
AvaII GGWCC 4 cut(s) 118, 219, 648, 729
BaeGI GKGCMC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BanI GGYRCC 1 cut(s) 767
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BbsI GAAGAC 2 cut(s) 45, 590
BbvI GCAGC 2 cut(s) 34, 647
BccI CCATC 4 cut(s) 89, 141, 267, 637
BceAI ACGGC 2 cut(s) 120, 161
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 1 cut(s) 414
BcnI CCSGG 1 cut(s) 657
BfaI CTAG 3 cut(s) 327, 345, 371
BisI GCNGC 3 cut(s) 23, 145, 661
BlsI GCNGC 3 cut(s) 24, 146, 662
Bme1390I CCNGG 2 cut(s) 414, 657
Bme18I GGWCC 4 cut(s) 118, 219, 648, 729
BmgT120I GGNCC 5 cut(s) 118, 219, 440, 648, 729
BmiI GGNNCC 2 cut(s) 649, 769
BmrFI CCNGG 2 cut(s) 414, 657
BmsI GCATC 3 cut(s) 52, 469, 563
BpiI GAAGAC 2 cut(s) 45, 590
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 1 cut(s) 657
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 671
Bse118I RCCGGY 2 cut(s) 154, 182
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 12, 133, 278
BseLI CCNNNNNNNGG 2 cut(s) 143, 671
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 2 cut(s) 549, 618
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 34, 647
BseYI CCCAGC 1 cut(s) 359
BshFI GGCC 1 cut(s) 442
BshNI GGYRCC 1 cut(s) 767
BsiSI CCGG 4 cut(s) 121, 155, 183, 656
BslFI GGGAC 1 cut(s) 661
BslI CCNNNNNNNGG 2 cut(s) 143, 671
BsmFI GGGAC 1 cut(s) 661
BsnI GGCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 9, 699
BspACI CCGC 6 cut(s) 137, 144, 147, 158, 217, 264
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 649, 769
BspT107I GGYRCC 1 cut(s) 767
BsrFI RCCGGY 2 cut(s) 154, 182
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 2 cut(s) 154, 182
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 9, 699
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 127, 230, 248, 715
BstAPI GCANNNNNTGC 1 cut(s) 214
BstC8I GCNNGC 3 cut(s) 184, 335, 491
BstDEI CTNAG 1 cut(s) 355
BstF5I GGATG 3 cut(s) 12, 133, 278
BstHHI GCGC 2 cut(s) 333, 484
BstKTI GATC 2 cut(s) 12, 702
BstMBI GATC 2 cut(s) 9, 699
BstMWI GCNNNNNNNGC 2 cut(s) 214, 490
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 3 cut(s) 46, 317, 579
BstSCI CCNGG 2 cut(s) 412, 655
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 34, 647
BstV2I GAAGAC 2 cut(s) 45, 590
BsuRI GGCC 1 cut(s) 442
BtsCI GGATG 3 cut(s) 12, 133, 278
BtsIMutI CAGTG 2 cut(s) 68, 712
Cac8I GCNNGC 3 cut(s) 184, 335, 491
CfoI GCGC 2 cut(s) 333, 484
Cfr10I RCCGGY 2 cut(s) 154, 182
Cfr13I GGNCC 5 cut(s) 118, 219, 440, 648, 729
Csp6I GTAC 3 cut(s) 560, 675, 768
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 4 cut(s) 43, 314, 576, 680
CviQI GTAC 3 cut(s) 560, 675, 768
DdeI CTNAG 1 cut(s) 355
DpnI GATC 2 cut(s) 11, 701
DpnII GATC 2 cut(s) 9, 699
DraI TTTAAA 1 cut(s) 408
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 222
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 4 cut(s) 118, 219, 648, 729
EcoRII CCWGG 1 cut(s) 412
EcoRV GATATC 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 4 cut(s) 46, 317, 579, 683
FaqI GGGAC 1 cut(s) 661
FatI CATG 4 cut(s) 42, 313, 575, 679
FauNDI CATATG 2 cut(s) 445, 565
Fnu4HI GCNGC 3 cut(s) 23, 145, 661
FokI GGATG 2 cut(s) 120, 285
Fsp4HI GCNGC 3 cut(s) 23, 145, 661
FspBI CTAG 3 cut(s) 327, 345, 371
FspI TGCGCA 1 cut(s) 483
GlaI GCGC 2 cut(s) 332, 483
GluI GCNGC 3 cut(s) 23, 145, 661
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 442
HapII CCGG 4 cut(s) 121, 155, 183, 656
HhaI GCGC 2 cut(s) 333, 484
Hin1II CATG 4 cut(s) 46, 317, 579, 683
Hin6I GCGC 2 cut(s) 331, 482
HinP1I GCGC 2 cut(s) 331, 482
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HindIII AAGCTT 2 cut(s) 50, 335
HinfI GANTC 3 cut(s) 56, 323, 741
HpaII CCGG 4 cut(s) 121, 155, 183, 656
HphI GGTGA 4 cut(s) 101, 278, 413, 602
Hpy166II GTNNAC 2 cut(s) 152, 238
Hpy188I TCNGA 4 cut(s) 61, 190, 688, 697
Hpy188III TCNNGA 3 cut(s) 531, 602, 745
Hpy8I GTNNAC 2 cut(s) 152, 238
Hpy99I CGWCG 3 cut(s) 176, 182, 206
HpyAV CCTTC 2 cut(s) 535, 742
HpyCH4III ACNGT 4 cut(s) 127, 230, 248, 715
HpyCH4V TGCA 4 cut(s) 208, 313, 521, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 490
HpyF3I CTNAG 1 cut(s) 355
Hsp92II CATG 4 cut(s) 46, 317, 579, 683
HspAI GCGC 2 cut(s) 331, 482
KpnI GGTACC 1 cut(s) 771
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 9, 699
Lsp1109I GCAGC 2 cut(s) 34, 647
LweI GCATC 3 cut(s) 52, 469, 563
MaeI CTAG 3 cut(s) 327, 345, 371
MaeIII GTNAC 1 cut(s) 709
MalI GATC 2 cut(s) 11, 701
MboI GATC 2 cut(s) 9, 699
MboII GAAGA 6 cut(s) 50, 104, 397, 581, 590, 652
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 259, 340
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 7 cut(s) 184, 262, 295, 444, 527, 530, 639
Mph1103I ATGCAT 1 cut(s) 523
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 2 cut(s) 339, 407
MslI CAYNNNNRTG 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 4 cut(s) 121, 155, 183, 656
MspR9I CCNGG 2 cut(s) 414, 657
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 214, 490
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 1 cut(s) 657
NdeI CATATG 2 cut(s) 445, 565
NdeII GATC 2 cut(s) 9, 699
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 4 cut(s) 46, 317, 579, 683
NlaIV GGNNCC 2 cut(s) 649, 769
NmeAIII GCCGAG 1 cut(s) 307
NsbI TGCGCA 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 523
NspI RCATGY 3 cut(s) 46, 317, 579
PciI ACATGT 1 cut(s) 42
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 2 cut(s) 56, 741
PkrI GCNGC 3 cut(s) 24, 146, 662
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 412
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 649, 769
PspPI GGNCC 5 cut(s) 118, 219, 440, 648, 729
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 561, 676, 769
RsaNI GTAC 3 cut(s) 560, 675, 768
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 2 cut(s) 339, 407
SatI GCNGC 3 cut(s) 23, 145, 661
Sau3AI GATC 2 cut(s) 9, 699
Sau96I GGNCC 5 cut(s) 118, 219, 440, 648, 729
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 414, 657
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 3 cut(s) 52, 469, 563
SinI GGWCC 4 cut(s) 118, 219, 648, 729
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
Sse9I AATT 2 cut(s) 259, 340
SsiI CCGC 6 cut(s) 137, 144, 147, 158, 217, 264
SspMI CTAG 3 cut(s) 327, 345, 371
StyD4I CCNGG 2 cut(s) 412, 655
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 4 cut(s) 127, 230, 248, 715
TaqI TCGA 1 cut(s) 530
TasI AATT 2 cut(s) 259, 340
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 56, 741
Tru1I TTAA 2 cut(s) 339, 407
Tru9I TTAA 2 cut(s) 339, 407
TscAI CASTG 2 cut(s) 75, 712
TseI GCWGC 2 cut(s) 22, 660
TspDTI ATGAA 2 cut(s) 398, 582
TspGWI ACGGA 2 cut(s) 115, 742
TspRI CASTG 2 cut(s) 75, 712
VpaK11BI GGWCC 4 cut(s) 118, 219, 648, 729
XceI RCATGY 3 cut(s) 46, 317, 579
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 3 cut(s) 327, 345, 371
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.