Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009334

Physical Location & Seq

Genomic Coordinates Forward (+)

13675703 .. 13678597

Gene Structure 2895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1169000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGGATTCGAGCAGCTCGGGCTCCACGAAGCTTGATTACTTCGACGACACGTGGAAGCTCCAATCCACCGCCACATTGCTATCCGTATCTAAAGGGGAAGATGGACGAACCGCCTTGGTATTGGACCGGACGATCTTCTATCCCCAAGGTGGCGGGCAACCTGCCGACACCGGCTTCATCGCCGCTTCCGATTCCGGGGTCAAATTCGTAGTAGAAGATGTGCGGTCCCGAGACGGCATAGTTTACCACTACGGTTTCATTGAAAATTCCTGTGAGGAATCGGAGTCGAAATTCGAGAAAGGGAAAGAGGTGCTGTTGTGTGTGGATGAGTCTAGGCGCAGGCTTAATGCTAGGCTTCACTCAGCTGGGCATTTGCTGGATACATGTATGAGAAATATGGGATTGGGTGATTTAGAGCCAACCAAAGGCTACCATTTCCCTCAGGGGCCATATGTGGAATATAAAGGCACAGTTCCACAAAAAGATACAGAAAGTAAGCGAAAGGAGTTGGAGGTAGAAGCTAATGTGTTAATATCGAGAGGAGGAAAAGTTTCTGCTGCCTTGTTACCATATGAAGAAGCGTGTAAGCTGTGTGGTGATTGCCTTCCCGATTATATTGCAAAGGACAGCACTCCTCGCATTGTGAAGCTAGGGGACAGCCTTGGCTGCCCATGTGGCGGTACCCATGTTTCTGATATATCAGAGATCAAGAGCATTACGGTTACTCAAGTACGGACAAAGAAAGGATCGACAAAGGTCTTTTACAATATCGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000049 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0031406 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

tRNA-synt_2c tRNA_SAD

Protein Analysis

259

Amino Acids

28.18

Weight (kDa)

5.81

Isoelectric Point (pI)

47.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	36 - 111	5.6e-12	tRNA synthetases class II (A)
tRNA_SAD	PF07973	213 - 248	3.1e-06	Threonyl and Alanyl tRNA synthetase second additional domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009334)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16565 AT3G16565 AT3G16565 AT3G16565 AT3G16565 AT3G16565
fragaria_vesca	FvH4_4g17960
malus_domestica	MD13G1169000.v1.1 MD16G1169600.v1.1
prunus_persica	Prupe.1G137400_v2.0.a1
pyrus_communis	pycom13g14460
rosa_chinensis	RchiOBHm_Chr4g0422131
rosa_laevigata	RLG00000007632
rosa_multiflora	Rmu_sc0004902.1_g000006 Rmu_sc0036261.1_g000001
rosa_roxburghii	Rroxscaffold_5G00364210
rosa_rugosa	Rorug04G0177600
rosa_samantha	Rh4AG237100 Rh4BG239900 Rh4CG251800 Rh4DG236000
rosa_wichuraiana	Rw4G020510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	169
Acc65I	GGTACC	1 cut(s)	680
AccB1I	GGYRCC	1 cut(s)	680
AciI	CCGC	6 cut(s)	69, 111, 153, 183, 223, 678
AclWI	GGATC	1 cut(s)	754
AcsI	RAATTY	3 cut(s)	203, 265, 290
AcvI	CACGTG	1 cut(s)	51
AfaI	GTAC	2 cut(s)	682, 732
AfiI	CCNNNNNNNGG	4 cut(s)	149, 195, 425, 677
AflIII	ACRYGT	2 cut(s)	48, 383
AgsI	TTSAA	1 cut(s)	263
AluBI	AGCT	7 cut(s)	15, 31, 58, 365, 521, 589, 649
AluI	AGCT	7 cut(s)	15, 31, 58, 365, 521, 589, 649
Alw26I	GTCTC	1 cut(s)	225
AlwI	GGATC	1 cut(s)	754
Ama87I	CYCGRG	2 cut(s)	16, 228
AoxI	GGCC	1 cut(s)	446
ApeKI	GCWGC	3 cut(s)	12, 557, 666
ApoI	RAATTY	3 cut(s)	203, 265, 290
Asp700I	GAANNNNTTC	1 cut(s)	550
Asp718I	GGTACC	1 cut(s)	680
AspLEI	GCGC	1 cut(s)	339
AspS9I	GGNCC	3 cut(s)	124, 225, 446
AsuC2I	CCSGG	1 cut(s)	196
AsuHPI	GGTGA	2 cut(s)	419, 608
AvaI	CYCGRG	2 cut(s)	16, 228
AvaII	GGWCC	2 cut(s)	124, 225
AxyI	CCTNAGG	1 cut(s)	441
BaeI	ACNNNNGTAYC	2 cut(s)	672, 705
BanI	GGYRCC	1 cut(s)	680
BanII	GRGCYC	1 cut(s)	23
BarI	GAAGNNNNNNTAC	2 cut(s)	20, 52
BbrPI	CACGTG	1 cut(s)	51
BbvI	GCAGC	3 cut(s)	24, 544, 653
BccI	CCATC	1 cut(s)	95
BceAI	ACGGC	1 cut(s)	250
BcgI	CGANNNNNNTGC	2 cut(s)	599, 633
BciVI	GTATCC	1 cut(s)	373
BcnI	CCSGG	1 cut(s)	196
BcoDI	GTCTC	1 cut(s)	225
BfaI	CTAG	3 cut(s)	333, 351, 650
BfuAI	ACCTGC	1 cut(s)	169
BfuI	GTATCC	1 cut(s)	373
BglI	GCCNNNNNGGC	1 cut(s)	675
BisI	GCNGC	4 cut(s)	13, 183, 558, 667
BlsI	GCNGC	4 cut(s)	14, 184, 559, 668
Bme1390I	CCNGG	1 cut(s)	196
Bme18I	GGWCC	2 cut(s)	124, 225
BmeT110I	CYCGRG	2 cut(s)	16, 228
BmgT120I	GGNCC	3 cut(s)	124, 225, 446
BmiI	GGNNCC	4 cut(s)	22, 227, 447, 682
BmrFI	CCNGG	1 cut(s)	196
BoxI	GACNNNNGTC	1 cut(s)	755
BpuEI	CTTGAG	1 cut(s)	711
BpuMI	CCSGG	1 cut(s)	196
BsaAI	YACGTR	1 cut(s)	51
BsaJI	CCNNGG	4 cut(s)	114, 145, 195, 661
BsaWI	WCCGGW	1 cut(s)	126
BsaXI	ACNNNNNCTCC	2 cut(s)	534, 564
Bsc4I	CCNNNNNNNGG	4 cut(s)	149, 195, 425, 677
Bse118I	RCCGGY	1 cut(s)	170
Bse21I	CCTNAGG	1 cut(s)	441
Bse3DI	GCAATG	1 cut(s)	74
BseDI	CCNNGG	4 cut(s)	114, 145, 195, 661
BseGI	GGATG	1 cut(s)	331
BseLI	CCNNNNNNNGG	4 cut(s)	149, 195, 425, 677
BseMI	GCAATG	1 cut(s)	74
BseMII	CTCAG	2 cut(s)	375, 455
BseRI	GAGGAG	2 cut(s)	555, 624
BseXI	GCAGC	3 cut(s)	24, 544, 653
BseYI	CCCAGC	1 cut(s)	365
BshFI	GGCC	1 cut(s)	448
BshNI	GGYRCC	1 cut(s)	680
BsiHKCI	CYCGRG	2 cut(s)	16, 228
BsiSI	CCGG	3 cut(s)	127, 171, 195
BslFI	GGGAC	2 cut(s)	211, 668
BslI	CCNNNNNNNGG	4 cut(s)	149, 195, 425, 677
BsmAI	GTCTC	1 cut(s)	225
BsmBI	CGTCTC	1 cut(s)	225
BsmFI	GGGAC	2 cut(s)	211, 668
BsnI	GGCC	1 cut(s)	448
BsoBI	CYCGRG	2 cut(s)	16, 228
Bsp1286I	GDGCHC	1 cut(s)	23
Bsp143I	GATC	3 cut(s)	132, 705, 746
BspACI	CCGC	6 cut(s)	69, 111, 153, 183, 223, 678
BspANI	GGCC	1 cut(s)	448
BspCNI	CTCAG	2 cut(s)	374, 454
BspLI	GGNNCC	4 cut(s)	22, 227, 447, 682
BspMI	ACCTGC	1 cut(s)	169
BspPI	GGATC	1 cut(s)	754
BspT107I	GGYRCC	1 cut(s)	680
BsrDI	GCAATG	1 cut(s)	74
BsrFI	RCCGGY	1 cut(s)	170
BssAI	RCCGGY	1 cut(s)	170
BssECI	CCNNGG	4 cut(s)	114, 145, 195, 661
BssMI	GATC	3 cut(s)	132, 705, 746
BssT1I	CCWWGG	3 cut(s)	114, 145, 661
Bst4CI	ACNGT	3 cut(s)	254, 472, 721
BstBAI	YACGTR	1 cut(s)	51
BstC8I	GCNNGC	2 cut(s)	155, 341
BstDEI	CTNAG	2 cut(s)	361, 441
BstF5I	GGATG	1 cut(s)	331
BstHHI	GCGC	1 cut(s)	339
BstKTI	GATC	3 cut(s)	135, 708, 749
BstMAI	GTCTC	1 cut(s)	225
BstMBI	GATC	3 cut(s)	132, 705, 746
BstMWI	GCNNNNNNNGC	4 cut(s)	18, 636, 666, 675
BstNSI	RCATGY	1 cut(s)	387
BstPAI	GACNNNNGTC	1 cut(s)	755
BstSCI	CCNGG	1 cut(s)	194
BstV1I	GCAGC	3 cut(s)	24, 544, 653
Bsu36I	CCTNAGG	1 cut(s)	441
BsuI	GTATCC	1 cut(s)	373
BsuRI	GGCC	1 cut(s)	448
BtgZI	GCGATG	1 cut(s)	163
BtsCI	GGATG	1 cut(s)	331
BveI	ACCTGC	1 cut(s)	169
Cac8I	GCNNGC	2 cut(s)	155, 341
CfoI	GCGC	1 cut(s)	339
Cfr10I	RCCGGY	1 cut(s)	170
Cfr13I	GGNCC	3 cut(s)	124, 225, 446
Csp6I	GTAC	2 cut(s)	681, 731
CspCI	CAANNNNNGTGG	2 cut(s)	13, 48
CviAII	CATG	3 cut(s)	384, 672, 686
CviQI	GTAC	2 cut(s)	681, 731
DdeI	CTNAG	2 cut(s)	361, 441
DpnI	GATC	3 cut(s)	134, 707, 748
DpnII	GATC	3 cut(s)	132, 705, 746
Eco130I	CCWWGG	3 cut(s)	114, 145, 661
Eco24I	GRGCYC	1 cut(s)	23
Eco47I	GGWCC	2 cut(s)	124, 225
Eco72I	CACGTG	1 cut(s)	51
Eco81I	CCTNAGG	1 cut(s)	441
Eco88I	CYCGRG	2 cut(s)	16, 228
EcoT14I	CCWWGG	3 cut(s)	114, 145, 661
EcoT38I	GRGCYC	1 cut(s)	23
ErhI	CCWWGG	3 cut(s)	114, 145, 661
Esp3I	CGTCTC	1 cut(s)	225
FaeI	CATG	3 cut(s)	387, 675, 689
FaqI	GGGAC	2 cut(s)	211, 668
FatI	CATG	3 cut(s)	383, 671, 685
FauI	CCCGC	1 cut(s)	146
FauNDI	CATATG	2 cut(s)	451, 571
Fnu4HI	GCNGC	4 cut(s)	13, 183, 558, 667
FokI	GGATG	1 cut(s)	338
FriOI	GRGCYC	1 cut(s)	23
Fsp4HI	GCNGC	4 cut(s)	13, 183, 558, 667
FspBI	CTAG	3 cut(s)	333, 351, 650
GlaI	GCGC	1 cut(s)	338
GluI	GCNGC	4 cut(s)	13, 183, 558, 667
GsaI	CCCAGC	1 cut(s)	369
HaeIII	GGCC	1 cut(s)	448
HapII	CCGG	3 cut(s)	127, 171, 195
HhaI	GCGC	1 cut(s)	339
Hin1II	CATG	3 cut(s)	387, 675, 689
Hin6I	GCGC	1 cut(s)	337
HinP1I	GCGC	1 cut(s)	337
HindIII	AAGCTT	1 cut(s)	29
HinfI	GANTC	5 cut(s)	5, 191, 278, 284, 329
HpaII	CCGG	3 cut(s)	127, 171, 195
HphI	GGTGA	2 cut(s)	419, 608
Hpy166II	GTNNAC	1 cut(s)	244
Hpy188I	TCNGA	4 cut(s)	190, 283, 694, 703
Hpy188III	TCNNGA	5 cut(s)	228, 295, 537, 608, 709
Hpy8I	GTNNAC	1 cut(s)	244
Hpy99I	CGWCG	1 cut(s)	47
HpyAV	CCTTC	1 cut(s)	614
HpyCH4III	ACNGT	3 cut(s)	254, 472, 721
HpyCH4IV	ACGT	1 cut(s)	50
HpyCH4V	TGCA	1 cut(s)	620
HpyF10VI	GCNNNNNNNGC	4 cut(s)	18, 636, 666, 675
HpyF3I	CTNAG	2 cut(s)	361, 441
HpySE526I	ACGT	1 cut(s)	50
Hsp92II	CATG	3 cut(s)	387, 675, 689
HspAI	GCGC	1 cut(s)	337
KpnI	GGTACC	1 cut(s)	684
Kzo9I	GATC	3 cut(s)	132, 705, 746
LmnI	GCTCC	2 cut(s)	26, 63
LpnPI	CCDG	9 cut(s)	140, 174, 184, 208, 283, 325, 351, 362, 428
Lsp1109I	GCAGC	3 cut(s)	24, 544, 653
MaeI	CTAG	3 cut(s)	333, 351, 650
MaeII	ACGT	1 cut(s)	50
MaeIII	GTNAC	2 cut(s)	564, 721
MalI	GATC	3 cut(s)	134, 707, 748
MboI	GATC	3 cut(s)	132, 705, 746
MboII	GAAGA	4 cut(s)	110, 127, 227, 587
MhlI	GDGCHC	1 cut(s)	23
MluCI	AATT	3 cut(s)	203, 265, 290
MlyI	GAGTC	2 cut(s)	293, 338
MmeI	TCCRAC	1 cut(s)	489
MnlI	CCTC	7 cut(s)	268, 301, 450, 505, 533, 536, 645
MroXI	GAANNNNTTC	1 cut(s)	550
MseI	TTAA	2 cut(s)	345, 530
MspA1I	CMGCKG	1 cut(s)	365
MspI	CCGG	3 cut(s)	127, 171, 195
MspR9I	CCNGG	1 cut(s)	196
MwoI	GCNNNNNNNGC	4 cut(s)	18, 636, 666, 675
NciI	CCSGG	1 cut(s)	196
NdeI	CATATG	2 cut(s)	451, 571
NdeII	GATC	3 cut(s)	132, 705, 746
NlaIII	CATG	3 cut(s)	387, 675, 689
NlaIV	GGNNCC	4 cut(s)	22, 227, 447, 682
NspI	RCATGY	1 cut(s)	387
PciI	ACATGT	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	2 cut(s)	23, 186
PdmI	GAANNNNTTC	1 cut(s)	550
PfeI	GAWTC	3 cut(s)	5, 191, 278
PkrI	GCNGC	4 cut(s)	14, 184, 559, 668
PleI	GAGTC	2 cut(s)	292, 337
PmaCI	CACGTG	1 cut(s)	51
PmlI	CACGTG	1 cut(s)	51
PpsI	GAGTC	2 cut(s)	292, 337
Ppu21I	YACGTR	1 cut(s)	51
PscI	ACATGT	1 cut(s)	383
PshAI	GACNNNNGTC	1 cut(s)	755
PspCI	CACGTG	1 cut(s)	51
PspFI	CCCAGC	1 cut(s)	365
PspN4I	GGNNCC	4 cut(s)	22, 227, 447, 682
PspPI	GGNCC	3 cut(s)	124, 225, 446
PvuII	CAGCTG	1 cut(s)	365
RsaI	GTAC	2 cut(s)	682, 732
RsaNI	GTAC	2 cut(s)	681, 731
SaqAI	TTAA	2 cut(s)	345, 530
SatI	GCNGC	4 cut(s)	13, 183, 558, 667
Sau3AI	GATC	3 cut(s)	132, 705, 746
Sau96I	GGNCC	3 cut(s)	124, 225, 446
SchI	GAGTC	2 cut(s)	293, 338
ScrFI	CCNGG	1 cut(s)	196
SduI	GDGCHC	1 cut(s)	23
SinI	GGWCC	2 cut(s)	124, 225
SmlI	CTYRAG	1 cut(s)	726
SmoI	CTYRAG	1 cut(s)	726
Sse9I	AATT	3 cut(s)	203, 265, 290
SsiI	CCGC	6 cut(s)	69, 111, 153, 183, 223, 678
SspMI	CTAG	3 cut(s)	333, 351, 650
StyD4I	CCNGG	1 cut(s)	194
StyI	CCWWGG	3 cut(s)	114, 145, 661
TaaI	ACNGT	3 cut(s)	254, 472, 721
TaiI	ACGT	1 cut(s)	53
TaqI	TCGA	6 cut(s)	8, 42, 287, 294, 536, 749
TasI	AATT	3 cut(s)	203, 265, 290
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	3 cut(s)	5, 191, 278
Tru1I	TTAA	2 cut(s)	345, 530
Tru9I	TTAA	2 cut(s)	345, 530
TseI	GCWGC	3 cut(s)	12, 557, 666
TspDTI	ATGAA	3 cut(s)	166, 247, 588
TspGWI	ACGGA	2 cut(s)	73, 748
VpaK11BI	GGWCC	2 cut(s)	124, 225
XapI	RAATTY	3 cut(s)	203, 265, 290
XceI	RCATGY	1 cut(s)	387
XmnI	GAANNNNTTC	1 cut(s)	550
XspI	CTAG	3 cut(s)	333, 351, 650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.