Prupe.1G137400_v2.0.a1

tRNA synthetases class II (A)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009334
Physical Location & Seq
Forward (+)
10758561 .. 10760949
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G137400.1

Sequence Viewer

Length: 780 bp
ATGGCTTCGAGCAGCTTGAGCCCCACGAAGCTCGATTATTTCGACGACATGGGGAAGCTCCAGTCCAGAGCCATATTGCTATCTTTATCCAAAGGTGAAGATGGACGAACCGCTTTGGTATTGGACCGTACAATTTTCCATCCACAAGGTGGTGGCCAACCTGCCGACACAGGCTTCATCGCCGTCGCAGATTCCGGTCTCAAATTCGTAGCAGAAGATGTACGGTCCAAAGACGGCATAGTTTACCACTACGGTTTTGTTGAAAATTCTGTTGAGGAAATGGAATCAAAATTTGAGAAAGGGAAAGAGGTGTTCTTGCGTGTTGATGAGGCTAGGCGCAAGCTAAATTCTAGGCTTCACTCAGCTGGGCATTTGCTGGATGCATGTATGAGAAATGTGGGATTGGGTGATTTAGAGCCAACCAAAGGCTACCATTTCCCTCAGGGGCCATATGTGGAATACAAAGGCACCATTCCACAAAAAGATATGGAAATTAAGCAAAAGGAGTTGGAGCTAGAAGCTAATGCATTAATCTCGAGAGGAGGGAAAGTTTCTGGTGCCTTGTTACCATATGAAGAAGCGTGTAAACTGTGTGGTGATTTCCTTCCCGATTACATTCCCAAGGGCAGCACTCCTCGCATTGTGAAGCTAGGGGACAGTCCTGGCTGTCCGTGTGGTGGTACCCATGTTTCTGATATTTCAGAGATCAAGAGTCTTACGGTTTCTCAAATACGAACAAAGAAAGGATCCACAAAGGTGTTCTACAATGTTGGGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA-synt_2c tRNA_SAD

Protein Analysis

260

Amino Acids

28.24

Weight (kDa)

6.61

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 169
Acc65I GGTACC 1 cut(s) 680
AccB1I GGYRCC 3 cut(s) 467, 557, 680
AccB7I CCANNNNNTGG 1 cut(s) 149
AciI CCGC 1 cut(s) 111
AclWI GGATC 2 cut(s) 741, 754
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 4 cut(s) 203, 265, 290, 346
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 3 cut(s) 130, 222, 682
AfiI CCNNNNNNNGG 3 cut(s) 149, 425, 677
AgsI TTSAA 1 cut(s) 263
AjnI CCWGG 1 cut(s) 661
AleI CACNNNNGTG 1 cut(s) 755
AluBI AGCT 8 cut(s) 15, 31, 58, 343, 365, 514, 521, 649
AluI AGCT 8 cut(s) 15, 31, 58, 343, 365, 514, 521, 649
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 2 cut(s) 741, 754
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 2 cut(s) 154, 446
ApeKI GCWGC 2 cut(s) 12, 627
ApoI RAATTY 4 cut(s) 203, 265, 290, 346
AseI ATTAAT 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 680
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 4 cut(s) 124, 225, 446, 774
AsuHPI GGTGA 3 cut(s) 107, 419, 608
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 3 cut(s) 124, 225, 774
AxyI CCTNAGG 1 cut(s) 441
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BalI TGGCCA 1 cut(s) 156
BamHI GGATCC 1 cut(s) 746
BanI GGYRCC 3 cut(s) 467, 557, 680
BanII GRGCYC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 24, 639
BccI CCATC 2 cut(s) 95, 147
BceAI ACGGC 2 cut(s) 167, 250
BcgI CGANNNNNNTGC 2 cut(s) 516, 550
BciT130I CCWGG 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 4 cut(s) 333, 351, 515, 650
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 2 cut(s) 13, 628
BlsI GCNGC 2 cut(s) 14, 629
Bme1390I CCNGG 1 cut(s) 663
Bme18I GGWCC 3 cut(s) 124, 225, 774
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 4 cut(s) 124, 225, 446, 774
BmiI GGNNCC 6 cut(s) 447, 469, 559, 682, 748, 775
BmrFI CCNGG 1 cut(s) 663
BmsI GCATC 1 cut(s) 370
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 37
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 621
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 534, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 425, 677
Bse1I ACTGG 1 cut(s) 61
Bse21I CCTNAGG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 2 cut(s) 139, 385
BseLI CCNNNNNNNGG 3 cut(s) 149, 425, 677
BseMII CTCAG 2 cut(s) 375, 455
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 2 cut(s) 555, 624
BseXI GCAGC 2 cut(s) 24, 639
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 2 cut(s) 156, 448
BshNI GGYRCC 3 cut(s) 467, 557, 680
BsiHKCI CYCGRG 1 cut(s) 535
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 3 cut(s) 149, 425, 677
BsmAI GTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 2 cut(s) 156, 448
Bso31I GGTCTC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 2 cut(s) 705, 746
BspACI CCGC 1 cut(s) 111
BspANI GGCC 2 cut(s) 156, 448
BspCNI CTCAG 2 cut(s) 374, 454
BspLI GGNNCC 6 cut(s) 447, 469, 559, 682, 748, 775
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 2 cut(s) 741, 754
BspT107I GGYRCC 3 cut(s) 467, 557, 680
BspTNI GGTCTC 1 cut(s) 203
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 2 cut(s) 705, 746
BssT1I CCWWGG 1 cut(s) 621
Bst2UI CCWGG 1 cut(s) 663
Bst4CI ACNGT 6 cut(s) 128, 225, 254, 591, 659, 721
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 361, 441
BstF5I GGATG 2 cut(s) 139, 385
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 2 cut(s) 708, 749
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 2 cut(s) 705, 746
BstMWI GCNNNNNNNGC 2 cut(s) 18, 636
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 661
BstV1I GCAGC 2 cut(s) 24, 639
BstX2I RGATCY 1 cut(s) 746
BstYI RGATCY 1 cut(s) 746
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 2 cut(s) 156, 448
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 2 cut(s) 139, 385
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 341
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 4 cut(s) 124, 225, 446, 774
Csp6I GTAC 3 cut(s) 129, 221, 681
CviAII CATG 3 cut(s) 49, 384, 686
CviQI GTAC 3 cut(s) 129, 221, 681
DdeI CTNAG 2 cut(s) 361, 441
DpnI GATC 2 cut(s) 707, 748
DpnII GATC 2 cut(s) 705, 746
DraIII CACNNNGTG 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 3 cut(s) 124, 225, 774
Eco81I CCTNAGG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 774
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 621
EcoT22I ATGCAT 2 cut(s) 385, 529
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 3 cut(s) 52, 387, 689
FaqI GGGAC 1 cut(s) 668
FatI CATG 3 cut(s) 48, 383, 685
FauNDI CATATG 2 cut(s) 451, 571
Fnu4HI GCNGC 2 cut(s) 13, 628
FokI GGATG 2 cut(s) 126, 392
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 13, 628
FspBI CTAG 4 cut(s) 333, 351, 515, 650
GlaI GCGC 1 cut(s) 338
GluI GCNGC 2 cut(s) 13, 628
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 2 cut(s) 156, 448
HapII CCGG 1 cut(s) 195
HhaI GCGC 1 cut(s) 339
Hin1II CATG 3 cut(s) 52, 387, 689
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HinfI GANTC 3 cut(s) 191, 284, 712
HpaII CCGG 1 cut(s) 195
HphI GGTGA 3 cut(s) 107, 419, 608
Hpy166II GTNNAC 2 cut(s) 244, 587
Hpy188I TCNGA 2 cut(s) 694, 703
Hpy188III TCNNGA 5 cut(s) 66, 535, 537, 608, 709
Hpy8I GTNNAC 2 cut(s) 244, 587
Hpy99I CGWCG 2 cut(s) 47, 188
HpyAV CCTTC 1 cut(s) 614
HpyCH4III ACNGT 6 cut(s) 128, 225, 254, 591, 659, 721
HpyCH4V TGCA 2 cut(s) 383, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 636
HpyF3I CTNAG 2 cut(s) 361, 441
Hsp92II CATG 3 cut(s) 52, 387, 689
HspAI GCGC 1 cut(s) 337
KpnI GGTACC 1 cut(s) 684
Kzo9I GATC 2 cut(s) 705, 746
LmnI GCTCC 2 cut(s) 63, 511
Lsp1109I GCAGC 2 cut(s) 24, 639
LweI GCATC 1 cut(s) 370
MaeI CTAG 4 cut(s) 333, 351, 515, 650
MaeIII GTNAC 1 cut(s) 564
MalI GATC 2 cut(s) 707, 748
MboI GATC 2 cut(s) 705, 746
MboII GAAGA 3 cut(s) 110, 227, 587
MflI RGATCY 1 cut(s) 746
MhlI GDGCHC 1 cut(s) 23
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 6 cut(s) 132, 203, 265, 290, 346, 492
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 1 cut(s) 721
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 7 cut(s) 268, 301, 322, 450, 533, 536, 645
Mox20I TGGCCA 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 385, 529
MscI TGGCCA 1 cut(s) 156
MseI TTAA 2 cut(s) 495, 530
MslI CAYNNNNRTG 1 cut(s) 755
Msp20I TGGCCA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 1 cut(s) 663
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 2 cut(s) 18, 636
NdeI CATATG 2 cut(s) 451, 571
NdeII GATC 2 cut(s) 705, 746
NlaIII CATG 3 cut(s) 52, 387, 689
NlaIV GGNNCC 6 cut(s) 447, 469, 559, 682, 748, 775
NsiI ATGCAT 2 cut(s) 385, 529
NspI RCATGY 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 755
PaeR7I CTCGAG 1 cut(s) 535
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 2 cut(s) 191, 284
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 2 cut(s) 14, 629
PleI GAGTC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 720
PpuMI RGGWCCY 1 cut(s) 774
PshBI ATTAAT 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 774
Psp6I CCWGG 1 cut(s) 661
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 661
PspN4I GGNNCC 6 cut(s) 447, 469, 559, 682, 748, 775
PspPI GGNCC 4 cut(s) 124, 225, 446, 774
PspPPI RGGWCCY 1 cut(s) 774
PsuI RGATCY 1 cut(s) 746
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 3 cut(s) 130, 222, 682
RsaNI GTAC 3 cut(s) 129, 221, 681
RseI CAYNNNNRTG 1 cut(s) 755
SaqAI TTAA 2 cut(s) 495, 530
SatI GCNGC 2 cut(s) 13, 628
Sau3AI GATC 2 cut(s) 705, 746
Sau96I GGNCC 4 cut(s) 124, 225, 446, 774
SchI GAGTC 1 cut(s) 721
ScrFI CCNGG 1 cut(s) 663
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 370
Sfr274I CTCGAG 1 cut(s) 535
SinI GGWCC 3 cut(s) 124, 225, 774
SlaI CTCGAG 1 cut(s) 535
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 2 cut(s) 16, 535
SmoI CTYRAG 2 cut(s) 16, 535
Sse9I AATT 6 cut(s) 132, 203, 265, 290, 346, 492
SsiI CCGC 1 cut(s) 111
SspMI CTAG 4 cut(s) 333, 351, 515, 650
StyD4I CCNGG 1 cut(s) 661
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 6 cut(s) 128, 225, 254, 591, 659, 721
TaqI TCGA 4 cut(s) 8, 33, 42, 536
TasI AATT 6 cut(s) 132, 203, 265, 290, 346, 492
TfiI GAWTC 2 cut(s) 191, 284
Tru1I TTAA 2 cut(s) 495, 530
Tru9I TTAA 2 cut(s) 495, 530
TseI GCWGC 2 cut(s) 12, 627
TspDTI ATGAA 2 cut(s) 166, 588
TspGWI ACGGA 1 cut(s) 660
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 124, 225, 774
VspI ATTAAT 1 cut(s) 530
XapI RAATTY 4 cut(s) 203, 265, 290, 346
XceI RCATGY 1 cut(s) 387
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 535
XspI CTAG 4 cut(s) 333, 351, 515, 650
Zsp2I ATGCAT 2 cut(s) 385, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.