AT3G19895

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008096
Physical Location & Seq
Reverse (-)
6917242 .. 6921600
4359 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19895.1

Sequence Viewer

Length: 1665 bp
ATGCACCGGCAGGGCTCGTCTCCGATTACGCCTTCAAACTATAACGCCGCCGTTTCAATTGCTTCTGATGTACCTACTGGTTTAACCAAGTCTCCTTTATCTCACGCGATTCCAGTTTCGCCGATTTCTGCTGCTTACCCTTCCGGTGATCCTATTTTGATGATGTCTGGGACTCCTCTCGCTAGGGTTCGATTATCTGAAATTTTGCCTTATGAAGGAGCTCCTTCTCCGGTGTATGCAAAAGCTGTGGAGGCTTTGTCTGTGTCTTTGATGAGGTATAATGCGTCTGTGATCGAGATAGGGAGTGAAGACACTGCACTGATGCGATGTGGCCTTGAAGCTGCTCGTTTGTATTTTAGAACTAGAAGCCTTACTGTTTCTGGTAAAGGCAATCGTGGCCTCTCTATGTATAGAGCCGGAAGGTCTGTTGAAGATTTAGATTCTTCACCTCCATGCATGGCTGAGATTTTCAGGTGTTTGGGTAAAGTAGCACGAGCTGCTTTATCTGCAATAGCAAGGCATCTACGTTTAAGAAGCGATGTTTTTAACCATATGCTTGATGACTTTCCACTAGCTCCGAACGAGGTTTCCTCCTCGGTGCTACTGGCTTCCTATGCTCATGCATCGATACAAAATGGAAAACATGCTTCTGGAGGTGGGAACCTATCTGCTAAGATTGAAGTTGAGAAGGGTTTGTTGACACTGTTCTGCTCAGATGGCACTGGCATTCAGGTATGTGACCCAAATGGTCGTTGGTACACAGCAGATAATGGATGTGGGGTGGGTGATCTCTTACTGATTACCGGCAAAGCGCTTAGTCATGCTACTGCAGGTCTCCGTCCAGCTGCCTCTTACAGAACTACTACCGATCATTTGTCTGCCACTGACACTCGAGGAAGGGCATCATTAGCTTTTAGGCTTATGCCGAAAAGTAATGCTATATTAGATTGTTCCCCAATAGAAGCAGCCGGCCATGTAATTCCTCAAAGCTATGTGCCAGTATCTGTTAGTCAGTTTATGGATAACCTCTTAGCCGAGAATGACACTCTAGTTAATCCGCCTGTGAAAACCAATGTTCCACGGGATGATGTTTGCAAAGAGCCTTCCTTAAGAAGTGTTCTCTCGGATCCAATTTCTGGTGCTTTTCTTGAAGATGCAATGGTTGTCTCTTGTGGACATTCATTTGGTGGCCTTATGCTTAGAAGAGTCCTTGAAATGTCTAGATGTACGCTCTGCAATACAGAGATCGAGTCTGGGTCTCTGGTTCCTAACCATGCACTTAGAGCTGCCGCTTCAGCTATCAAGCAACAGGATGATAAACGACTTTTCCACAACGCAGCCATGAGAAGGCGTAGAAAAGAAATGAGTGATCAAATGGATGTGGAAAATGGAGATCCAGCTACGGATGATGGTATGCATCGAGTGGTGCATTATCCATTTGCTGTAAATGAGAAAGTTCTTATCAAGGGAAACCGGCGAACTCCAGAAAAGTTTGTTGGAAAAGAAGCAATCGTGACATCTCAGTGTCTCAATGGCTGGTATCTATTAAAGATTGTCGAAAGCGGAGACAATGTACGGCTGCAATACCGATCCCTAAAAAAGATGGTGAACGATGACAGAGGAGGAGGCCTACAGGCTCAACCGGTTGAGAGCAATAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

554

Amino Acids

59.69

Weight (kDa)

8.37

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB62-63_C PF23112 476 - 534 7.7e-36 U-box protein 62/63 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 1136
AccII CGCG 1 cut(s) 107
AciI CCGC 4 cut(s) 48, 1058, 1290, 1563
AclWI GGATC 5 cut(s) 143, 1121, 1134, 1388, 1584
AcoI YGGCCR 1 cut(s) 970
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 1278
AfaI GTAC 4 cut(s) 72, 758, 1228, 1575
AfeI AGCGCT 1 cut(s) 813
AfiI CCNNNNNNNGG 3 cut(s) 215, 1136, 1347
AflII CTTAAG 1 cut(s) 1108
AgeI ACCGGT 1 cut(s) 1642
AgsI TTSAA 7 cut(s) 36, 57, 338, 431, 680, 1151, 1214
AjuI GAANNNNNNNTTGG 2 cut(s) 1479, 1511
AloI GAACNNNNNNTCC 2 cut(s) 572, 604
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 7 cut(s) 24, 96, 839, 1171, 1263, 1532, 1560
AlwI GGATC 5 cut(s) 143, 1121, 1134, 1388, 1584
AlwNI CAGNNNCTG 1 cut(s) 1004
Ama87I CYCGRG 1 cut(s) 891
Aor51HI AGCGCT 1 cut(s) 813
AoxI GGCC 5 cut(s) 331, 397, 970, 1189, 1627
ApeKI GCWGC 8 cut(s) 131, 341, 497, 845, 965, 1286, 1337, 1579
ApoI RAATTY 1 cut(s) 201
AsiGI ACCGGT 1 cut(s) 1642
AspLEI GCGC 1 cut(s) 814
AsuHPI GGTGA 4 cut(s) 158, 438, 797, 1618
AvaI CYCGRG 1 cut(s) 891
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 2 cut(s) 17, 223
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 8 cut(s) 118, 328, 484, 832, 977, 1273, 1349, 1566
BccI CCATC 3 cut(s) 710, 1403, 1597
BceAI ACGGC 2 cut(s) 35, 1592
BclI TGATCA 1 cut(s) 1369
BcoDI GTCTC 7 cut(s) 24, 96, 839, 1171, 1263, 1532, 1560
BfaI CTAG 5 cut(s) 183, 363, 572, 1049, 1221
BfmI CTRYAG 2 cut(s) 828, 1631
BfoI RGCGCY 1 cut(s) 815
BfrI CTTAAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 821
BmeT110I CYCGRG 1 cut(s) 891
BmiI GGNNCC 3 cut(s) 662, 1128, 1266
BmsI GCATC 6 cut(s) 312, 529, 632, 911, 1144, 1426
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 575, 607
BpmI CTGGAG 2 cut(s) 672, 1467
Bsa29I ATCGAT 1 cut(s) 626
BsaI GGTCTC 2 cut(s) 839, 1263
BsaJI CCNNGG 2 cut(s) 594, 1079
BsaWI WCCGGW 3 cut(s) 143, 229, 1642
BsaXI ACNNNNNCTCC 6 cut(s) 76, 106, 242, 272, 1557, 1587
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 1136, 1347
Bse118I RCCGGY 5 cut(s) 6, 803, 968, 1473, 1642
Bse1I ACTGG 5 cut(s) 82, 113, 609, 727, 998
Bse3DI GCAATG 1 cut(s) 1164
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 2 cut(s) 594, 1079
BseGI GGATG 5 cut(s) 779, 1090, 1318, 1384, 1411
BseLI CCNNNNNNNGG 3 cut(s) 215, 1136, 1347
BseMI GCAATG 1 cut(s) 1164
BseMII CTCAG 3 cut(s) 453, 726, 1535
BseNI ACTGG 5 cut(s) 82, 113, 609, 727, 998
BseRI GAGGAG 4 cut(s) 165, 583, 1635, 1638
BseXI GCAGC 8 cut(s) 118, 328, 484, 832, 977, 1273, 1349, 1566
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 5 cut(s) 333, 399, 972, 1191, 1629
BshTI ACCGGT 1 cut(s) 1642
BshVI ATCGAT 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 891
BsiSI CCGG 8 cut(s) 7, 144, 230, 417, 804, 969, 1474, 1643
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 215, 1136, 1347
BsmAI GTCTC 7 cut(s) 24, 96, 839, 1171, 1263, 1532, 1560
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 5 cut(s) 333, 399, 972, 1191, 1629
Bso31I GGTCTC 2 cut(s) 839, 1263
BsoBI CYCGRG 1 cut(s) 891
Bsp1286I GDGCHC 2 cut(s) 17, 223
Bsp143I GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
BspACI CCGC 4 cut(s) 48, 1058, 1290, 1563
BspANI GGCC 5 cut(s) 333, 399, 972, 1191, 1629
BspCNI CTCAG 3 cut(s) 454, 725, 1534
BspDI ATCGAT 1 cut(s) 626
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 662, 1128, 1266
BspMAI CTGCAG 1 cut(s) 832
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 5 cut(s) 143, 1121, 1134, 1388, 1584
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 2 cut(s) 839, 1263
BsrDI GCAATG 1 cut(s) 1164
BsrFI RCCGGY 5 cut(s) 6, 803, 968, 1473, 1642
BsrI ACTGG 5 cut(s) 82, 113, 609, 727, 998
BssAI RCCGGY 5 cut(s) 6, 803, 968, 1473, 1642
BssECI CCNNGG 2 cut(s) 594, 1079
BssMI GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
BssSI CACGAG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 376, 705
Bst6I CTCTTC 1 cut(s) 1198
BstAFI CTTAAG 1 cut(s) 1108
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 970
BstDEI CTNAG 8 cut(s) 462, 672, 712, 815, 1030, 1199, 1280, 1521
BstDSI CCRYGG 1 cut(s) 1079
BstENI CCTNNNNNAGG 1 cut(s) 213
BstF5I GGATG 5 cut(s) 779, 1090, 1318, 1384, 1411
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 815
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 9 cut(s) 151, 294, 790, 871, 1129, 1248, 1372, 1396, 1592
BstMAI GTCTC 7 cut(s) 24, 96, 839, 1171, 1263, 1532, 1560
BstMBI GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
BstMWI GCNNNNNNNGC 9 cut(s) 251, 396, 497, 506, 614, 717, 908, 1283, 1295
BstNSI RCATGY 1 cut(s) 647
BstSFI CTRYAG 2 cut(s) 828, 1631
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 8 cut(s) 118, 328, 484, 832, 977, 1273, 1349, 1566
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 2 cut(s) 1126, 1393
BstYI RGATCY 2 cut(s) 1126, 1393
Bsu15I ATCGAT 1 cut(s) 626
BsuRI GGCC 5 cut(s) 333, 399, 972, 1191, 1629
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 1079
BtgZI GCGATG 2 cut(s) 340, 552
BtsCI GGATG 5 cut(s) 779, 1090, 1318, 1384, 1411
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 6 cut(s) 312, 317, 701, 720, 882, 1529
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 1 cut(s) 970
CaiI CAGNNNCTG 1 cut(s) 1004
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 5 cut(s) 6, 803, 968, 1473, 1642
ClaI ATCGAT 1 cut(s) 626
CseI GACGC 1 cut(s) 273
Csp6I GTAC 4 cut(s) 71, 757, 1227, 1574
CspAI ACCGGT 1 cut(s) 1642
CspCI CAANNNNNGTGG 2 cut(s) 228, 263
CviAII CATG 8 cut(s) 453, 457, 620, 644, 821, 974, 1274, 1342
CviQI GTAC 4 cut(s) 71, 757, 1227, 1574
DdeI CTNAG 8 cut(s) 462, 672, 712, 815, 1030, 1199, 1280, 1521
DpnI GATC 9 cut(s) 150, 293, 789, 870, 1128, 1247, 1371, 1395, 1591
DpnII GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
EaeI YGGCCR 1 cut(s) 970
Eam1104I CTCTTC 1 cut(s) 1198
EarI CTCTTC 1 cut(s) 1198
EciI GGCGGA 1 cut(s) 1047
Ecl136II GAGCTC 1 cut(s) 221
Eco147I AGGCCT 1 cut(s) 1629
Eco24I GRGCYC 2 cut(s) 17, 223
Eco31I GGTCTC 2 cut(s) 839, 1263
Eco47III AGCGCT 1 cut(s) 813
Eco53kI GAGCTC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 1278
Eco88I CYCGRG 1 cut(s) 891
EcoICRI GAGCTC 1 cut(s) 221
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoT22I ATGCAT 3 cut(s) 458, 625, 1419
EcoT38I GRGCYC 2 cut(s) 17, 223
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 8 cut(s) 456, 460, 623, 647, 824, 977, 1277, 1345
FaqI GGGAC 1 cut(s) 184
FatI CATG 8 cut(s) 452, 456, 619, 643, 820, 973, 1273, 1341
FauNDI CATATG 1 cut(s) 552
FbaI TGATCA 1 cut(s) 1369
FokI GGATG 5 cut(s) 786, 1097, 1325, 1391, 1418
FriOI GRGCYC 2 cut(s) 17, 223
FspBI CTAG 5 cut(s) 183, 363, 572, 1049, 1221
GlaI GCGC 1 cut(s) 813
GsuI CTGGAG 2 cut(s) 672, 1467
HaeII RGCGCY 1 cut(s) 815
HaeIII GGCC 5 cut(s) 333, 399, 972, 1191, 1629
HapII CCGG 8 cut(s) 7, 144, 230, 417, 804, 969, 1474, 1643
HgaI GACGC 1 cut(s) 273
HhaI GCGC 1 cut(s) 814
Hin1II CATG 8 cut(s) 456, 460, 623, 647, 824, 977, 1277, 1345
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HinfI GANTC 5 cut(s) 109, 172, 440, 1206, 1250
HpaII CCGG 8 cut(s) 7, 144, 230, 417, 804, 969, 1474, 1643
HphI GGTGA 4 cut(s) 158, 438, 797, 1618
Hpy166II GTNNAC 4 cut(s) 699, 759, 1175, 1609
Hpy188I TCNGA 6 cut(s) 24, 67, 199, 579, 715, 1126
Hpy188III TCNNGA 6 cut(s) 295, 651, 1148, 1221, 1484, 1513
Hpy8I GTNNAC 4 cut(s) 699, 759, 1175, 1609
HpyAV CCTTC 9 cut(s) 42, 150, 209, 234, 414, 682, 891, 1113, 1341
HpyCH4III ACNGT 2 cut(s) 376, 705
HpyCH4IV ACGT 1 cut(s) 526
HpyF10VI GCNNNNNNNGC 9 cut(s) 251, 396, 497, 506, 614, 717, 908, 1283, 1295
HpyF3I CTNAG 8 cut(s) 462, 672, 712, 815, 1030, 1199, 1280, 1521
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 8 cut(s) 456, 460, 623, 647, 824, 977, 1277, 1345
HspAI GCGC 1 cut(s) 812
KroI GCCGGC 1 cut(s) 968
KroNI GCCGGC 1 cut(s) 970
Ksp22I TGATCA 1 cut(s) 1369
Kzo9I GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
LmnI GCTCC 3 cut(s) 218, 226, 580
Lsp1109I GCAGC 8 cut(s) 118, 328, 484, 832, 977, 1273, 1349, 1566
LweI GCATC 6 cut(s) 312, 529, 632, 911, 1144, 1426
MaeI CTAG 5 cut(s) 183, 363, 572, 1049, 1221
MaeII ACGT 1 cut(s) 526
MaeIII GTNAC 2 cut(s) 737, 1513
MalI GATC 9 cut(s) 150, 293, 789, 870, 1128, 1247, 1371, 1395, 1591
MboI GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
MboII GAAGA 5 cut(s) 320, 435, 443, 1163, 1215
MfeI CAATTG 1 cut(s) 57
MflI RGATCY 2 cut(s) 1126, 1393
MhlI GDGCHC 2 cut(s) 17, 223
MluCI AATT 4 cut(s) 57, 201, 978, 1131
MlyI GAGTC 3 cut(s) 166, 1215, 1259
MmeI TCCRAC 1 cut(s) 1477
Mph1103I ATGCAT 3 cut(s) 458, 625, 1419
MroNI GCCGGC 1 cut(s) 968
MseI TTAA 6 cut(s) 83, 530, 546, 1053, 1109, 1547
MslI CAYNNNNRTG 2 cut(s) 451, 1522
MspA1I CMGCKG 1 cut(s) 845
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 8 cut(s) 7, 144, 230, 417, 804, 969, 1474, 1643
MunI CAATTG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 726
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 9 cut(s) 251, 396, 497, 506, 614, 717, 908, 1283, 1295
NaeI GCCGGC 1 cut(s) 970
NdeI CATATG 1 cut(s) 552
NdeII GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
NgoMIV GCCGGC 1 cut(s) 968
NlaIII CATG 8 cut(s) 456, 460, 623, 647, 824, 977, 1277, 1345
NlaIV GGNNCC 3 cut(s) 662, 1128, 1266
NmeAIII GCCGAG 1 cut(s) 1060
NmuCI GTSAC 2 cut(s) 737, 1513
NsiI ATGCAT 3 cut(s) 458, 625, 1419
NspI RCATGY 1 cut(s) 647
PaeR7I CTCGAG 1 cut(s) 891
PceI AGGCCT 1 cut(s) 1629
PctI GAATGC 1 cut(s) 726
PdiI GCCGGC 1 cut(s) 970
PfeI GAWTC 2 cut(s) 109, 440
PflMI CCANNNNNTGG 1 cut(s) 1136
PinAI ACCGGT 1 cut(s) 1642
PleI GAGTC 3 cut(s) 166, 1214, 1258
PpsI GAGTC 3 cut(s) 166, 1214, 1258
Psp124BI GAGCTC 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 662, 1128, 1266
PspXI VCTCGAGB 1 cut(s) 891
PstI CTGCAG 1 cut(s) 832
PstNI CAGNNNCTG 1 cut(s) 1004
PsuI RGATCY 2 cut(s) 1126, 1393
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 4 cut(s) 72, 758, 1228, 1575
RsaNI GTAC 4 cut(s) 71, 757, 1227, 1574
RseI CAYNNNNRTG 2 cut(s) 451, 1522
SacI GAGCTC 1 cut(s) 223
SaqAI TTAA 6 cut(s) 83, 530, 546, 1053, 1109, 1547
Sau3AI GATC 9 cut(s) 148, 291, 787, 868, 1126, 1245, 1369, 1393, 1589
SchI GAGTC 3 cut(s) 166, 1215, 1259
SduI GDGCHC 2 cut(s) 17, 223
SfaNI GCATC 6 cut(s) 312, 529, 632, 911, 1144, 1426
SfcI CTRYAG 2 cut(s) 828, 1631
Sfr274I CTCGAG 1 cut(s) 891
SlaI CTCGAG 1 cut(s) 891
SmiMI CAYNNNNRTG 2 cut(s) 451, 1522
SmlI CTYRAG 2 cut(s) 891, 1108
SmoI CTYRAG 2 cut(s) 891, 1108
Sse9I AATT 4 cut(s) 57, 201, 978, 1131
SseBI AGGCCT 1 cut(s) 1629
SsiI CCGC 4 cut(s) 48, 1058, 1290, 1563
SspMI CTAG 5 cut(s) 183, 363, 572, 1049, 1221
SstI GAGCTC 1 cut(s) 223
StuI AGGCCT 1 cut(s) 1629
TaaI ACNGT 2 cut(s) 376, 705
TaiI ACGT 1 cut(s) 529
TaqI TCGA 7 cut(s) 190, 294, 626, 892, 1248, 1420, 1557
TasI AATT 4 cut(s) 57, 201, 978, 1131
TauI GCSGC 2 cut(s) 50, 1292
TfiI GAWTC 2 cut(s) 109, 440
Tru1I TTAA 6 cut(s) 83, 530, 546, 1053, 1109, 1547
Tru9I TTAA 6 cut(s) 83, 530, 546, 1053, 1109, 1547
TscAI CASTG 6 cut(s) 319, 324, 708, 727, 889, 1529
TseFI GTSAC 2 cut(s) 737, 1513
TseI GCWGC 8 cut(s) 131, 341, 497, 845, 965, 1286, 1337, 1579
Tsp45I GTSAC 2 cut(s) 737, 1513
TspDTI ATGAA 2 cut(s) 228, 1170
TspGWI ACGGA 2 cut(s) 827, 1418
TspRI CASTG 6 cut(s) 319, 324, 708, 727, 889, 1529
Van91I CCANNNNNTGG 1 cut(s) 1136
Vha464I CTTAAG 1 cut(s) 1108
XagI CCTNNNNNAGG 1 cut(s) 213
XapI RAATTY 1 cut(s) 201
XbaI TCTAGA 1 cut(s) 1220
XceI RCATGY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 750
XhoI CTCGAG 1 cut(s) 891
XspI CTAG 5 cut(s) 183, 363, 572, 1049, 1221
Zsp2I ATGCAT 3 cut(s) 458, 625, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.