MD13G1008400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008096
Physical Location & Seq
Reverse (-)
514055 .. 518195
4141 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1008400.v1.1.491

Sequence Viewer

Length: 1686 bp
ATGGTCGTGATCATGATGCAGCAGCACAACTCTGCAGCACCCGCGTCGCACTCAATCACTACTCCCCCCTCCTCCGCCACCACCCCCACCGCCATTAGATCACCCACGGCAACCCCTACACACTCCCAGCAGCACCCCTCGCCTCTCCCCCGCCATGCTCTTCCTGTTCCGCCGCCTCTCGACCACGTCCTGCCGATTGCGGCCGCCCACCCGTCCGGCGAGCCTGCGCCGACAATCCAATGCCAGCTTGCGAGGGTCCGGCTCTCCGATATTACCCCGTACCACGGGGCACCTGGCGGGTCGTATGTGAAGGCGGTGGATGCGCTCTCGGGGTCTCTGACGAGGCACAATGCGGCCGTCATCGAATTGGGCAGCGACGACACGGCGCTCCTTAGGTGCGGCTTGGAAGCCTCCAGGTTGTATTTTAGGAGTAGAGCTCAGCTCGGTGTTGGGAAAGGAAGCCGTGGAGTTCACATGTACAGAGCTGGCAGGGCTTTGGAAGATTGGGACTCATCCCCGCCATGCATGGCGGATATTTTTAGGTGCATGGGCAAGGCGGCCCACGTGGCTTTGTGTGCAATAGCTAGGCATCTTCGTTTGCGAAGCAATGTTTTCAACCATTTACTTGACGATACTCCATTGCCCGCGAATGAGGTGTCCTCATCAGTTCTTGTTGCAACCTACTCAAATGCTTCCTTGCAAAATGGAAAAGGTGCTATTGGGGGAGGGAAGCCAGGAATGAATGCTGAAGTTGAGAAGGGATTGTTAACATTGGTTTCCTCAGACAGTCCTGGTATACAGGTATGTGACCCCAATGGTCGTTGGTACATAGCAGATGGTGGGTCAGCTCCTGGGGATCTTTTACTGCTTACAGGCAAGGCACTCAGCCATGCTACTGCTGGCCTTCGCCCTGCTGCACCATATAAAGCTGCACCTGATTACTCCTCTGGCATTAGTAGTGGTGGGAGGGCATCGCTTGCATTTAGGCTCATGCCGCAAGGTAATGCTATATTAGATTGTTCTCCAATTGCAGCGGCTGGTCATGTCATTCCCCACAGCTATGTCCCAATATCTGTAAGCCAGTTTATGGATGATCTTTCTGCTGAGGAAGATGTATTGGGCAGTCGTTCAGATAACACTTATGTAGCTCGAAACAGTTTAAACAAGGAACCATCTTTAAGAAGTGTTCTCTCAGATCCCGTATCTGGGTCATTCCTCGAAGATGCTATGTTAGTTTCTTGTGGGCATTCATTCGGTGGTCTCATGTTGAGAAGAGTCATTGAAACATCAAGATGCACCCTCTGCTGTGCAGAAATTGAGACCGGGTCTTTGGTTCCTAATCTTGCGCTTAGAGCTGCAGCTGCAGCAGTGAAGCATGAGGATGATCGAAGGCTATTTCATAATGCGACTGTCCGAAAGCGTCGGAAAGAATTGGTTGACCAAATGGCTTCAAGAAGACCAAACAAGGAAAATGGAGATATTGGTGATGATGGGATTCGTGGGGTGCAGTACCCGTTTTCTGTGAATGAAAAAGTGGTAATTAAGGGGAACAGGAGGACTCCAGAAAAATTTGTTGGCAAGGAAGCGGTCATCACATCCCAGTGTCTGAATGGCTGGTACTTGCTCAAGATTATTGCAACTGGAGAGAATGTTCGCTTGCAATACCGTTCCCTGCGGAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

562

Amino Acids

59.79

Weight (kDa)

9.01

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB62-63_C PF23112 502 - 560 3.9e-36 U-box protein 62/63 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 1087
AccI GTMKAC 1 cut(s) 796
AccII CGCG 2 cut(s) 44, 647
AclWI GGATC 2 cut(s) 864, 1190
AcoI YGGCCR 2 cut(s) 201, 354
AcsI RAATTY 1 cut(s) 1568
AcuI CTGAAG 1 cut(s) 768
AcvI CACGTG 1 cut(s) 565
AfaI GTAC 5 cut(s) 281, 479, 827, 1511, 1619
AfiI CCNNNNNNNGG 4 cut(s) 284, 1087, 1205, 1206
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 3 cut(s) 616, 1283, 1452
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 5 cut(s) 292, 413, 733, 790, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 1557, 1589
AloI GAACNNNNNNTCC 2 cut(s) 1635, 1667
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 3 cut(s) 339, 1265, 1313
AlwI GGATC 2 cut(s) 864, 1190
AlwNI CAGNNNCTG 3 cut(s) 851, 1037, 1606
Ama87I CYCGRG 1 cut(s) 328
AoxI GGCC 4 cut(s) 201, 354, 558, 901
ApoI RAATTY 1 cut(s) 1568
ArsI GACNNNNNNTTYG 4 cut(s) 641, 673, 1312, 1344
AspLEI GCGC 4 cut(s) 229, 325, 388, 1348
AspS9I GGNCC 2 cut(s) 256, 559
AsuC2I CCSGG 1 cut(s) 1324
AsuHPI GGTGA 2 cut(s) 93, 1496
AvaI CYCGRG 1 cut(s) 328
AvaII GGWCC 1 cut(s) 256
AxyI CCTNAGG 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 439
BbrPI CACGTG 1 cut(s) 565
BbsI GAAGAC 1 cut(s) 1462
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 1104
BccI CCATC 3 cut(s) 830, 1180, 1484
BceAI ACGGC 4 cut(s) 123, 341, 399, 447
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 5 cut(s) 294, 415, 735, 792, 852
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 1 cut(s) 1324
BcoDI GTCTC 3 cut(s) 339, 1265, 1313
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 3 cut(s) 33, 1356, 1362
BfoI RGCGCY 1 cut(s) 389
BglI GCCNNNNNGGC 1 cut(s) 566
BlpI GCTNAGC 1 cut(s) 438
Bme1390I CCNGG 6 cut(s) 294, 415, 735, 792, 852, 1324
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 328
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 256, 559
BmiI GGNNCC 4 cut(s) 257, 291, 1170, 1335
BmrFI CCNGG 6 cut(s) 294, 415, 735, 792, 852, 1324
BmrI ACTGGG 1 cut(s) 1594
BmsI GCATC 6 cut(s) 6, 310, 598, 980, 1213, 1283
BmuI ACTGGG 1 cut(s) 1594
BpiI GAAGAC 1 cut(s) 1462
BplI GAGNNNNNCTC 6 cut(s) 421, 453, 426, 458, 644, 676
BpmI CTGGAG 3 cut(s) 397, 1545, 1662
Bpu10I CCTNAGC 1 cut(s) 1104
Bpu1102I GCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 1610
BpuMI CCSGG 1 cut(s) 1324
BsaAI YACGTR 1 cut(s) 565
BsaI GGTCTC 3 cut(s) 339, 1265, 1313
BsaJI CCNNGG 4 cut(s) 105, 283, 463, 851
BsaXI ACNNNNNCTCC 2 cut(s) 1635, 1665
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 1087, 1205, 1206
Bse1I ACTGG 3 cut(s) 1081, 1600, 1645
Bse21I CCTNAGG 1 cut(s) 392
Bse3DI GCAATG 2 cut(s) 613, 638
BseBI CCWGG 5 cut(s) 294, 415, 735, 792, 852
BseDI CCNNGG 4 cut(s) 105, 283, 463, 851
BseGI GGATG 5 cut(s) 325, 512, 1096, 1387, 1595
BseLI CCNNNNNNNGG 4 cut(s) 284, 1087, 1205, 1206
BseMI GCAATG 2 cut(s) 613, 638
BseMII CTCAG 5 cut(s) 452, 795, 898, 1095, 1206
BseNI ACTGG 3 cut(s) 1081, 1600, 1645
BseRI GAGGAG 2 cut(s) 61, 934
BseSI GKGCMC 1 cut(s) 292
BseX3I CGGCCG 2 cut(s) 201, 354
BseYI CCCAGC 1 cut(s) 126
BsgI GTGCAG 4 cut(s) 900, 915, 1329, 1526
Bsh1236I CGCG 2 cut(s) 44, 647
Bsh1285I CGRYCG 2 cut(s) 204, 357
BshFI GGCC 4 cut(s) 203, 356, 560, 903
BshNI GGYRCC 1 cut(s) 289
BsiEI CGRYCG 2 cut(s) 204, 357
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 328
BsiSI CCGG 3 cut(s) 216, 259, 1323
BslFI GGGAC 2 cut(s) 521, 1049
BslI CCNNNNNNNGG 4 cut(s) 284, 1087, 1205, 1206
BsmAI GTCTC 3 cut(s) 339, 1265, 1313
BsmFI GGGAC 2 cut(s) 521, 1049
BsmI GAATGC 2 cut(s) 748, 1246
BsnI GGCC 4 cut(s) 203, 356, 560, 903
Bso31I GGTCTC 3 cut(s) 339, 1265, 1313
BsoBI CYCGRG 1 cut(s) 328
Bsp1286I GDGCHC 2 cut(s) 292, 439
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
Bsp1720I GCTNAGC 1 cut(s) 438
BspANI GGCC 4 cut(s) 203, 356, 560, 903
BspCNI CTCAG 5 cut(s) 451, 794, 897, 1096, 1205
BspFNI CGCG 2 cut(s) 44, 647
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 4 cut(s) 257, 291, 1170, 1335
BspMAI CTGCAG 3 cut(s) 37, 1360, 1366
BspPI GGATC 2 cut(s) 864, 1190
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 289
BspTNI GGTCTC 3 cut(s) 339, 1265, 1313
BsrDI GCAATG 2 cut(s) 613, 638
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 3 cut(s) 1081, 1600, 1645
BssECI CCNNGG 4 cut(s) 105, 283, 463, 851
BssMI GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
BssNAI GTATAC 1 cut(s) 797
Bst1107I GTATAC 1 cut(s) 797
Bst2UI CCWGG 5 cut(s) 294, 415, 735, 792, 852
Bst4CI ACNGT 4 cut(s) 788, 1157, 1411, 1667
Bst6I CTCTTC 2 cut(s) 165, 1267
BstAPI GCANNNNNTGC 2 cut(s) 977, 1302
BstAUI TGTACA 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 565
BstC8I GCNNGC 9 cut(s) 221, 225, 245, 249, 487, 645, 901, 978, 1658
BstDEI CTNAG 7 cut(s) 392, 438, 781, 884, 1104, 1192, 1349
BstDSI CCRYGG 3 cut(s) 105, 283, 463
BstF5I GGATG 5 cut(s) 325, 512, 1096, 1387, 1595
BstFNI CGCG 2 cut(s) 44, 647
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 4 cut(s) 229, 325, 388, 1348
BstKTI GATC 6 cut(s) 12, 101, 859, 1096, 1198, 1387
BstMAI GTCTC 3 cut(s) 339, 1265, 1313
BstMBI GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
BstMCI CGRYCG 2 cut(s) 204, 357
BstNI CCWGG 5 cut(s) 294, 415, 735, 792, 852
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 6 cut(s) 292, 413, 733, 790, 850, 1322
BstSFI CTRYAG 3 cut(s) 33, 1356, 1362
BstSLI GKGCMC 1 cut(s) 292
BstUI CGCG 2 cut(s) 44, 647
BstV2I GAAGAC 1 cut(s) 1462
BstX2I RGATCY 2 cut(s) 856, 1195
BstYI RGATCY 2 cut(s) 856, 1195
BstZ17I GTATAC 1 cut(s) 797
BstZI CGGCCG 2 cut(s) 201, 354
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 4 cut(s) 203, 356, 560, 903
BtgI CCRYGG 3 cut(s) 105, 283, 463
BtgZI GCGATG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 5 cut(s) 325, 512, 1096, 1387, 1595
BtsI GCAGTG 1 cut(s) 1374
BtsIMutI CAGTG 2 cut(s) 1374, 1607
Cac8I GCNNGC 9 cut(s) 221, 225, 245, 249, 487, 645, 901, 978, 1658
CaiI CAGNNNCTG 3 cut(s) 851, 1037, 1606
CciI TCATGA 1 cut(s) 12
CciNI GCGGCCGC 1 cut(s) 201
CfoI GCGC 4 cut(s) 229, 325, 388, 1348
Cfr13I GGNCC 2 cut(s) 256, 559
CseI GACGC 2 cut(s) 33, 1409
Csp6I GTAC 5 cut(s) 280, 478, 826, 1510, 1618
CviQI GTAC 5 cut(s) 280, 478, 826, 1510, 1618
DdeI CTNAG 7 cut(s) 392, 438, 781, 884, 1104, 1192, 1349
DpnI GATC 6 cut(s) 11, 100, 858, 1095, 1197, 1386
DpnII GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
DraI TTTAAA 1 cut(s) 1161
EaeI YGGCCR 2 cut(s) 201, 354
EagI CGGCCG 2 cut(s) 201, 354
Eam1104I CTCTTC 2 cut(s) 165, 1267
EarI CTCTTC 2 cut(s) 165, 1267
EciI GGCGGA 3 cut(s) 64, 159, 545
Ecl136II GAGCTC 1 cut(s) 437
EclXI CGGCCG 2 cut(s) 201, 354
Eco24I GRGCYC 1 cut(s) 439
Eco31I GGTCTC 3 cut(s) 339, 1265, 1313
Eco47I GGWCC 1 cut(s) 256
Eco52I CGGCCG 2 cut(s) 201, 354
Eco53kI GAGCTC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 768
Eco72I CACGTG 1 cut(s) 565
Eco81I CCTNAGG 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 437
EcoRII CCWGG 5 cut(s) 292, 413, 733, 790, 850
EcoT22I ATGCAT 1 cut(s) 527
EcoT38I GRGCYC 1 cut(s) 439
FaqI GGGAC 2 cut(s) 521, 1049
FauI CCCGC 5 cut(s) 49, 158, 290, 525, 652
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 796
FokI GGATG 5 cut(s) 332, 499, 1103, 1394, 1582
FriOI GRGCYC 1 cut(s) 439
FspBI CTAG 1 cut(s) 585
GlaI GCGC 4 cut(s) 228, 324, 387, 1347
GsaI CCCAGC 1 cut(s) 130
GsuI CTGGAG 3 cut(s) 397, 1545, 1662
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 4 cut(s) 203, 356, 560, 903
HapII CCGG 3 cut(s) 216, 259, 1323
HgaI GACGC 2 cut(s) 33, 1409
HhaI GCGC 4 cut(s) 229, 325, 388, 1348
Hin6I GCGC 4 cut(s) 227, 323, 386, 1346
HinP1I GCGC 4 cut(s) 227, 323, 386, 1346
HincII GTYRAC 2 cut(s) 768, 1438
HindII GTYRAC 2 cut(s) 768, 1438
HinfI GANTC 4 cut(s) 509, 1275, 1495, 1558
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 3 cut(s) 216, 259, 1323
HphI GGTGA 2 cut(s) 93, 1496
Hpy166II GTNNAC 4 cut(s) 472, 768, 797, 1438
Hpy188I TCNGA 8 cut(s) 268, 339, 784, 1132, 1195, 1415, 1425, 1608
Hpy188III TCNNGA 7 cut(s) 7, 13, 179, 1290, 1452, 1562, 1627
Hpy8I GTNNAC 4 cut(s) 472, 768, 797, 1438
Hpy99I CGWCG 3 cut(s) 49, 380, 1425
HpyAV CCTTC 4 cut(s) 304, 751, 914, 1383
HpyCH4III ACNGT 4 cut(s) 788, 1157, 1411, 1667
HpyCH4IV ACGT 2 cut(s) 186, 564
HpyF3I CTNAG 7 cut(s) 392, 438, 781, 884, 1104, 1192, 1349
HpySE526I ACGT 2 cut(s) 186, 564
HspAI GCGC 4 cut(s) 227, 323, 386, 1346
Ksp22I TGATCA 1 cut(s) 9
KspAI GTTAAC 1 cut(s) 768
Kzo9I GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 393, 853
LweI GCATC 6 cut(s) 6, 310, 598, 980, 1213, 1283
MaeI CTAG 1 cut(s) 585
MaeII ACGT 2 cut(s) 186, 564
MaeIII GTNAC 1 cut(s) 806
MalI GATC 6 cut(s) 11, 100, 858, 1095, 1197, 1386
MboI GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
MboII GAAGA 7 cut(s) 152, 512, 584, 1121, 1232, 1284, 1467
MfeI CAATTG 1 cut(s) 1026
MflI RGATCY 2 cut(s) 856, 1195
MhlI GDGCHC 2 cut(s) 292, 439
MluCI AATT 6 cut(s) 365, 1026, 1314, 1430, 1539, 1568
MlyI GAGTC 3 cut(s) 503, 1284, 1552
MmeI TCCRAC 1 cut(s) 1403
Mph1103I ATGCAT 1 cut(s) 527
MseI TTAA 4 cut(s) 767, 1160, 1178, 1542
MslI CAYNNNNRTG 4 cut(s) 1059, 1291, 1380, 1600
MspA1I CMGCKG 2 cut(s) 1034, 1361
MspI CCGG 3 cut(s) 216, 259, 1323
MspR9I CCNGG 6 cut(s) 294, 415, 735, 792, 852, 1324
MssI GTTTAAAC 1 cut(s) 1161
MunI CAATTG 1 cut(s) 1026
Mva1269I GAATGC 2 cut(s) 748, 1246
MvaI CCWGG 5 cut(s) 294, 415, 735, 792, 852
MvnI CGCG 2 cut(s) 44, 647
NciI CCSGG 1 cut(s) 1324
NdeII GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
NlaIV GGNNCC 4 cut(s) 257, 291, 1170, 1335
NmuCI GTSAC 1 cut(s) 806
NotI GCGGCCGC 1 cut(s) 201
NsiI ATGCAT 1 cut(s) 527
NspI RCATGY 1 cut(s) 478
PagI TCATGA 1 cut(s) 12
PciI ACATGT 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 2 cut(s) 748, 1246
PfeI GAWTC 1 cut(s) 1495
PflFI GACNNNGTC 2 cut(s) 185, 1324
PflMI CCANNNNNTGG 1 cut(s) 1087
PleI GAGTC 3 cut(s) 503, 1283, 1552
PmaCI CACGTG 1 cut(s) 565
PmeI GTTTAAAC 1 cut(s) 1161
PmlI CACGTG 1 cut(s) 565
PpsI GAGTC 3 cut(s) 503, 1283, 1552
Ppu21I YACGTR 1 cut(s) 565
PscI ACATGT 1 cut(s) 474
Psp124BI GAGCTC 1 cut(s) 439
Psp6I CCWGG 5 cut(s) 292, 413, 733, 790, 850
PspCI CACGTG 1 cut(s) 565
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 5 cut(s) 292, 413, 733, 790, 850
PspN4I GGNNCC 4 cut(s) 257, 291, 1170, 1335
PspPI GGNCC 2 cut(s) 256, 559
PstI CTGCAG 3 cut(s) 37, 1360, 1366
PstNI CAGNNNCTG 3 cut(s) 851, 1037, 1606
PsuI RGATCY 2 cut(s) 856, 1195
PsyI GACNNNGTC 2 cut(s) 185, 1324
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 5 cut(s) 281, 479, 827, 1511, 1619
RsaNI GTAC 5 cut(s) 280, 478, 826, 1510, 1618
RseI CAYNNNNRTG 4 cut(s) 1059, 1291, 1380, 1600
SacI GAGCTC 1 cut(s) 439
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 4 cut(s) 767, 1160, 1178, 1542
Sau3AI GATC 6 cut(s) 9, 98, 856, 1093, 1195, 1384
Sau96I GGNCC 2 cut(s) 256, 559
SchI GAGTC 3 cut(s) 503, 1284, 1552
ScrFI CCNGG 6 cut(s) 294, 415, 735, 792, 852, 1324
SduI GDGCHC 2 cut(s) 292, 439
SfaNI GCATC 6 cut(s) 6, 310, 598, 980, 1213, 1283
SfcI CTRYAG 3 cut(s) 33, 1356, 1362
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 4 cut(s) 1059, 1291, 1380, 1600
SmlI CTYRAG 1 cut(s) 1625
SmoI CTYRAG 1 cut(s) 1625
Sse9I AATT 6 cut(s) 365, 1026, 1314, 1430, 1539, 1568
SspMI CTAG 1 cut(s) 585
SstI GAGCTC 1 cut(s) 439
StyD4I CCNGG 6 cut(s) 292, 413, 733, 790, 850, 1322
TaaI ACNGT 4 cut(s) 788, 1157, 1411, 1667
TaiI ACGT 2 cut(s) 189, 567
TaqI TCGA 5 cut(s) 180, 363, 1150, 1218, 1387
TasI AATT 6 cut(s) 365, 1026, 1314, 1430, 1539, 1568
TatI WGTACW 1 cut(s) 477
TauI GCSGC 8 cut(s) 175, 203, 206, 356, 402, 560, 997, 1037
TfiI GAWTC 1 cut(s) 1495
Tru1I TTAA 4 cut(s) 767, 1160, 1178, 1542
Tru9I TTAA 4 cut(s) 767, 1160, 1178, 1542
TscAI CASTG 2 cut(s) 1374, 1607
TseFI GTSAC 1 cut(s) 806
Tsp45I GTSAC 1 cut(s) 806
TspDTI ATGAA 4 cut(s) 755, 1239, 1388, 1542
TspRI CASTG 2 cut(s) 1374, 1607
Tth111I GACNNNGTC 2 cut(s) 185, 1324
Van91I CCANNNNNTGG 1 cut(s) 1087
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 1568
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 3 cut(s) 290, 896, 1607
XmiI GTMKAC 1 cut(s) 796
XspI CTAG 1 cut(s) 585
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.