AT3G20015

Protein ASPARTIC PROTEASE IN GUARD CELL

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014901
Physical Location & Seq
Reverse (-)
6978477 .. 6980523
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20015.1

Sequence Viewer

Length: 1413 bp
ATGCTCCTCCCTCTCTTCTTCTTCTTTCTCCATCTCCATCTCCATCTCTCTTCTTCTTCTTCAATCTCCTTCCCTGATTTTCAAATCATCGACGTTTTACAACCTCCTCTCACCGTCACTGCAACTCTTCCCGATTTCAACAACACCCATTTCTCCGATGAATCCAGTTCTAAGTACACTCTCCGTCTTCTCCACCGCGACAGATTCCCTTCAGTCACTTACCGTAACCACCACCACCGTCTCCATGCTCGTATGCGCAGAGACACAGACAGAGTCTCCGCCATTCTCCGCCGCATTTCTGGGAAAGTTATACCTTCTTCCGATTCTAGATACGAGGTTAACGATTTTGGGTCCGATATTGTTTCCGGTATGGATCAAGGAAGTGGTGAGTATTTCGTCAGAATCGGCGTCGGAAGTCCTCCGAGAGATCAGTACATGGTCATTGATTCCGGTAGCGATATGGTTTGGGTTCAATGTCAGCCTTGTAAGCTCTGTTACAAACAATCCGACCCGGTTTTTGACCCGGCTAAATCCGGGTCTTACACTGGAGTCTCTTGTGGCTCTTCCGTCTGTGACCGGATTGAAAACTCCGGTTGTCACTCCGGTGGGTGCCGTTACGAGGTCATGTACGGAGACGGGTCTTACACGAAGGGAACGTTAGCTCTCGAAACGCTGACGTTTGCTAAGACGGTGGTTAGAAACGTTGCGATGGGATGTGGTCACCGGAATAGAGGAATGTTTATTGGAGCTGCCGGTTTATTGGGTATCGGAGGCGGTTCAATGTCGTTTGTGGGTCAATTAAGCGGTCAAACCGGTGGTGCGTTCGGTTATTGTTTAGTGAGTCGTGGCACTGACTCGACCGGTTCATTGGTATTCGGGCGGGAAGCGTTGCCCGTTGGTGCCTCGTGGGTCCCGTTGGTCCGGAACCCGCGGGCTCCAAGTTTTTACTACGTTGGGCTCAAGGGCCTTGGAGTAGGAGGCGTCCGGATTCCGTTACCGGATGGTGTTTTTGACCTGACTGAAACCGGTGACGGTGGTGTGGTTATGGATACCGGAACAGCCGTCACAAGACTTCCCACGGCGGCTTACGTCGCTTTTCGAGATGGATTCAAGTCGCAGACAGCGAATTTGCCGCGGGCTTCCGGTGTATCGATATTCGACACGTGTTATGATCTGTCAGGGTTTGTTTCGGTACGAGTCCCGACCGTGTCGTTCTATTTCACGGAAGGTCCGGTTTTGACATTACCGGCGAGGAATTTTCTGATGCCGGTGGATGATTCGGGAACGTATTGTTTTGCGTTTGCGGCGTCTCCGACGGGTTTGTCAATAATAGGGAACATACAACAAGAAGGGATTCAAGTATCTTTCGACGGAGCCAATGGCTTTGTTGGTTTTGGCCCCAACGTTTGCTAG

Protein Analysis

470

Amino Acids

50.54

Weight (kDa)

6.82

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 130 - 466 5.2e-18 Eukaryotic aspartyl protease
TAXi_N PF14543 131 - 293 1.5e-47 Xylanase inhibitor N-terminal
TAXi_C PF14541 316 - 466 7.1e-33 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20015
fragaria_vesca FvH4_4g37260
malus_domestica MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica Prupe.1G350500_v2.0.a1
pyrus_communis pycom13g00180 pycom16g00070
rosa_chinensis RchiOBHm_Chr4g0447081
rosa_laevigata RLG00000005607
rosa_roxburghii Rroxscaffold_5G00387390
rosa_rugosa Rorug04G0377800
rosa_samantha Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana Rw4G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1321
Acc16I TGCGCA 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 609, 899
AccII CGCG 3 cut(s) 198, 931, 1135
AccIII TCCGGA 2 cut(s) 921, 984
AclI AACGTT 3 cut(s) 656, 702, 1404
AclWI GGATC 1 cut(s) 381
AcsI RAATTY 2 cut(s) 1126, 1255
AcuI CTGAAG 1 cut(s) 195
AcvI CACGTG 1 cut(s) 1164
AcyI GRCGYC 3 cut(s) 408, 981, 1307
AfaI GTAC 4 cut(s) 176, 434, 629, 1194
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 2 cut(s) 1161, 1163
AgeI ACCGGT 3 cut(s) 812, 860, 1025
AgsI TTSAA 8 cut(s) 63, 83, 139, 473, 584, 780, 1111, 1358
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 3 cut(s) 490, 662, 749
AluI AGCT 3 cut(s) 490, 662, 749
Alw26I GTCTC 6 cut(s) 245, 255, 280, 556, 627, 1314
AlwI GGATC 1 cut(s) 381
Aor13HI TCCGGA 2 cut(s) 921, 984
AoxI GGCC 2 cut(s) 964, 1396
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 2 cut(s) 1126, 1255
ArsI GACNNNNNNTTYG 4 cut(s) 500, 532, 1111, 1143
AsiGI ACCGGT 3 cut(s) 812, 860, 1025
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 6 cut(s) 351, 910, 919, 964, 1229, 1397
AsuC2I CCSGG 3 cut(s) 512, 524, 535
AsuHPI GGTGA 4 cut(s) 103, 398, 713, 1040
AvaII GGWCC 4 cut(s) 351, 910, 919, 1229
BanI GGYRCC 2 cut(s) 609, 899
BanII GRGCYC 2 cut(s) 937, 960
BauI CACGAG 1 cut(s) 904
BbrPI CACGTG 1 cut(s) 1164
BbsI GAAGAC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 736
BccI CCATC 6 cut(s) 39, 45, 51, 703, 995, 1097
BceAI ACGGC 3 cut(s) 597, 1046, 1095
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 3 cut(s) 512, 524, 535
BcoDI GTCTC 6 cut(s) 245, 255, 280, 556, 627, 1314
BfaI CTAG 2 cut(s) 327, 1411
BfuI GTATCC 1 cut(s) 1042
BisI GCNGC 5 cut(s) 292, 750, 1083, 1133, 1305
BlsI GCNGC 5 cut(s) 293, 751, 1084, 1134, 1306
Bme1390I CCNGG 3 cut(s) 512, 524, 535
Bme18I GGWCC 4 cut(s) 351, 910, 919, 1229
BmgT120I GGNCC 6 cut(s) 351, 910, 919, 964, 1229, 1397
BmiI GGNNCC 9 cut(s) 352, 611, 901, 911, 912, 926, 936, 1375, 1399
BmrFI CCNGG 3 cut(s) 512, 524, 535
BmsI GCATC 1 cut(s) 1254
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 3 cut(s) 512, 524, 535
Bsa29I ATCGAT 1 cut(s) 1151
BsaAI YACGTR 1 cut(s) 1164
BsaHI GRCGYC 3 cut(s) 408, 981, 1307
BsaJI CCNNGG 4 cut(s) 929, 967, 1077, 1133
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 6 cut(s) 752, 812, 860, 1025, 1246, 1267
Bse1I ACTGG 2 cut(s) 165, 550
BseAI TCCGGA 2 cut(s) 921, 984
BseCI ATCGAT 1 cut(s) 1151
BseDI CCNNGG 4 cut(s) 929, 967, 1077, 1133
BseGI GGATG 3 cut(s) 719, 1006, 1279
BseLI CCNNNNNNNGG 1 cut(s) 619
BseNI ACTGG 2 cut(s) 165, 550
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 736
Bsh1236I CGCG 3 cut(s) 198, 931, 1135
Bsh1285I CGRYCG 2 cut(s) 861, 1206
BshFI GGCC 2 cut(s) 966, 1398
BshNI GGYRCC 2 cut(s) 609, 899
BshTI ACCGGT 3 cut(s) 812, 860, 1025
BshVI ATCGAT 1 cut(s) 1151
BsiEI CGRYCG 2 cut(s) 861, 1206
BslFI GGGAC 2 cut(s) 896, 1184
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 6 cut(s) 245, 255, 280, 556, 627, 1314
BsmBI CGTCTC 3 cut(s) 245, 627, 1314
BsmFI GGGAC 2 cut(s) 896, 1184
BsnI GGCC 2 cut(s) 966, 1398
Bsp1286I GDGCHC 2 cut(s) 937, 960
Bsp13I TCCGGA 2 cut(s) 921, 984
Bsp143I GATC 3 cut(s) 373, 427, 1171
BspANI GGCC 2 cut(s) 966, 1398
BspDI ATCGAT 1 cut(s) 1151
BspEI TCCGGA 2 cut(s) 921, 984
BspFNI CGCG 3 cut(s) 198, 931, 1135
BspLI GGNNCC 9 cut(s) 352, 611, 901, 911, 912, 926, 936, 1375, 1399
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 568
BspT107I GGYRCC 2 cut(s) 609, 899
BsrFI RCCGGY 6 cut(s) 752, 812, 860, 1025, 1246, 1267
BsrI ACTGG 2 cut(s) 165, 550
BssAI RCCGGY 6 cut(s) 752, 812, 860, 1025, 1246, 1267
BssECI CCNNGG 4 cut(s) 929, 967, 1077, 1133
BssMI GATC 3 cut(s) 373, 427, 1171
BssNI GRCGYC 3 cut(s) 408, 981, 1307
BssSI CACGAG 1 cut(s) 904
BssT1I CCWWGG 1 cut(s) 967
Bst2BI CACGAG 1 cut(s) 904
Bst4CI ACNGT 6 cut(s) 115, 224, 239, 691, 1034, 1207
Bst6I CTCTTC 4 cut(s) 20, 55, 132, 568
BstACI GRCGYC 3 cut(s) 408, 981, 1307
BstBAI YACGTR 1 cut(s) 1164
BstC8I GCNNGC 2 cut(s) 933, 1137
BstDEI CTNAG 2 cut(s) 171, 684
BstDSI CCRYGG 3 cut(s) 929, 1077, 1133
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 3 cut(s) 719, 1006, 1279
BstFNI CGCG 3 cut(s) 198, 931, 1135
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 3 cut(s) 376, 430, 1174
BstMAI GTCTC 6 cut(s) 245, 255, 280, 556, 627, 1314
BstMBI GATC 3 cut(s) 373, 427, 1171
BstMCI CGRYCG 2 cut(s) 861, 1206
BstMWI GCNNNNNNNGC 3 cut(s) 487, 1091, 1304
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 510, 522, 533
BstUI CGCG 3 cut(s) 198, 931, 1135
BstV1I GCAGC 1 cut(s) 736
BstV2I GAAGAC 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 1151
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 2 cut(s) 966, 1398
BsuTUI ATCGAT 1 cut(s) 1151
BtgI CCRYGG 3 cut(s) 929, 1077, 1133
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 3 cut(s) 719, 1006, 1279
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 117, 543, 849
Cac8I GCNNGC 2 cut(s) 933, 1137
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 6 cut(s) 752, 812, 860, 1025, 1246, 1267
Cfr13I GGNCC 6 cut(s) 351, 910, 919, 964, 1229, 1397
Cfr42I CCGCGG 2 cut(s) 932, 1136
ClaI ATCGAT 1 cut(s) 1151
CseI GACGC 3 cut(s) 397, 970, 1296
Csp6I GTAC 4 cut(s) 175, 433, 628, 1193
CspAI ACCGGT 3 cut(s) 812, 860, 1025
CviAII CATG 3 cut(s) 245, 436, 625
CviQI GTAC 4 cut(s) 175, 433, 628, 1193
DdeI CTNAG 2 cut(s) 171, 684
DpnI GATC 3 cut(s) 375, 429, 1173
DpnII GATC 3 cut(s) 373, 427, 1171
DrdI GACNNNNNNGTC 1 cut(s) 1321
DseDI GACNNNNNNGTC 1 cut(s) 1321
Eam1104I CTCTTC 4 cut(s) 20, 55, 132, 568
EarI CTCTTC 4 cut(s) 20, 55, 132, 568
EciI GGCGGA 2 cut(s) 268, 278
Eco130I CCWWGG 1 cut(s) 967
Eco24I GRGCYC 2 cut(s) 937, 960
Eco47I GGWCC 4 cut(s) 351, 910, 919, 1229
Eco57I CTGAAG 1 cut(s) 195
Eco72I CACGTG 1 cut(s) 1164
Eco91I GGTNACC 1 cut(s) 719
EcoO109I RGGNCCY 2 cut(s) 910, 964
EcoO65I GGTNACC 1 cut(s) 719
EcoT14I CCWWGG 1 cut(s) 967
EcoT38I GRGCYC 2 cut(s) 937, 960
ErhI CCWWGG 1 cut(s) 967
Esp3I CGTCTC 3 cut(s) 245, 627, 1314
FaeI CATG 3 cut(s) 248, 439, 628
FaqI GGGAC 2 cut(s) 896, 1184
FatI CATG 3 cut(s) 244, 435, 624
FauI CCCGC 4 cut(s) 873, 924, 936, 1128
Fnu4HI GCNGC 5 cut(s) 292, 750, 1083, 1133, 1305
FokI GGATG 3 cut(s) 726, 1013, 1286
FriOI GRGCYC 2 cut(s) 937, 960
Fsp4HI GCNGC 5 cut(s) 292, 750, 1083, 1133, 1305
FspBI CTAG 2 cut(s) 327, 1411
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 1 cut(s) 257
GluI GCNGC 5 cut(s) 292, 750, 1083, 1133, 1305
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 2 cut(s) 966, 1398
HgaI GACGC 3 cut(s) 397, 970, 1296
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 3 cut(s) 408, 981, 1307
Hin1II CATG 3 cut(s) 248, 439, 628
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HpaI GTTAAC 1 cut(s) 340
HphI GGTGA 4 cut(s) 103, 398, 713, 1040
Hpy166II GTNNAC 2 cut(s) 177, 340
Hpy188III TCNNGA 8 cut(s) 131, 327, 665, 922, 985, 1100, 1201, 1281
Hpy8I GTNNAC 2 cut(s) 177, 340
Hpy99I CGWCG 5 cut(s) 95, 413, 1094, 1318, 1373
HpyAV CCTTC 6 cut(s) 79, 219, 324, 643, 1220, 1343
HpyCH4III ACNGT 6 cut(s) 115, 224, 239, 691, 1034, 1207
HpyCH4IV ACGT 9 cut(s) 93, 656, 677, 702, 951, 1089, 1163, 1286, 1404
HpyCH4V TGCA 1 cut(s) 122
HpyF10VI GCNNNNNNNGC 3 cut(s) 487, 1091, 1304
HpyF3I CTNAG 2 cut(s) 171, 684
HpySE526I ACGT 9 cut(s) 93, 656, 677, 702, 951, 1089, 1163, 1286, 1404
Hsp92I GRCGYC 3 cut(s) 408, 981, 1307
Hsp92II CATG 3 cut(s) 248, 439, 628
HspAI GCGC 1 cut(s) 256
KflI GGGWCCC 1 cut(s) 910
Kpn2I TCCGGA 2 cut(s) 921, 984
KspAI GTTAAC 1 cut(s) 340
KspI CCGCGG 2 cut(s) 932, 1136
Kzo9I GATC 3 cut(s) 373, 427, 1171
LguI GCTCTTC 1 cut(s) 568
LmnI GCTCC 4 cut(s) 9, 746, 940, 1373
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 1 cut(s) 1254
MaeI CTAG 2 cut(s) 327, 1411
MaeII ACGT 9 cut(s) 93, 656, 677, 702, 951, 1089, 1163, 1286, 1404
MalI GATC 3 cut(s) 375, 429, 1173
MboI GATC 3 cut(s) 373, 427, 1171
MhlI GDGCHC 2 cut(s) 937, 960
MluCI AATT 3 cut(s) 797, 1126, 1255
MlyI GAGTC 5 cut(s) 282, 558, 848, 850, 1206
MmeI TCCRAC 3 cut(s) 391, 531, 1337
MroI TCCGGA 2 cut(s) 921, 984
MroXI GAANNNNTTC 1 cut(s) 1353
MseI TTAA 2 cut(s) 339, 800
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 2 cut(s) 931, 1135
MspR9I CCNGG 3 cut(s) 512, 524, 535
MvnI CGCG 3 cut(s) 198, 931, 1135
MwoI GCNNNNNNNGC 3 cut(s) 487, 1091, 1304
NciI CCSGG 3 cut(s) 512, 524, 535
NdeII GATC 3 cut(s) 373, 427, 1171
NlaIII CATG 3 cut(s) 248, 439, 628
NlaIV GGNNCC 9 cut(s) 352, 611, 901, 911, 912, 926, 936, 1375, 1399
NmuCI GTSAC 7 cut(s) 115, 214, 572, 596, 719, 1028, 1063
NsbI TGCGCA 1 cut(s) 257
OliI CACNNNNGTG 1 cut(s) 603
PciSI GCTCTTC 1 cut(s) 568
PcsI WCGNNNNNNNCGW 4 cut(s) 339, 653, 911, 1121
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 9 cut(s) 161, 204, 323, 402, 446, 988, 1107, 1277, 1354
PflFI GACNNNGTC 2 cut(s) 272, 1207
PinAI ACCGGT 3 cut(s) 812, 860, 1025
PkrI GCNGC 5 cut(s) 293, 751, 1084, 1134, 1306
PleI GAGTC 5 cut(s) 281, 557, 848, 849, 1205
PmaCI CACGTG 1 cut(s) 1164
PmlI CACGTG 1 cut(s) 1164
PpsI GAGTC 5 cut(s) 281, 557, 848, 849, 1205
Ppu21I YACGTR 1 cut(s) 1164
PpuMI RGGWCCY 1 cut(s) 910
Psp1406I AACGTT 3 cut(s) 656, 702, 1404
Psp5II RGGWCCY 1 cut(s) 910
PspCI CACGTG 1 cut(s) 1164
PspEI GGTNACC 1 cut(s) 719
PspN4I GGNNCC 9 cut(s) 352, 611, 901, 911, 912, 926, 936, 1375, 1399
PspPI GGNCC 6 cut(s) 351, 910, 919, 964, 1229, 1397
PspPPI RGGWCCY 1 cut(s) 910
PsyI GACNNNGTC 2 cut(s) 272, 1207
RsaI GTAC 4 cut(s) 176, 434, 629, 1194
RsaNI GTAC 4 cut(s) 175, 433, 628, 1193
RseI CAYNNNNRTG 1 cut(s) 603
SacII CCGCGG 2 cut(s) 932, 1136
SapI GCTCTTC 1 cut(s) 568
SaqAI TTAA 2 cut(s) 339, 800
SatI GCNGC 5 cut(s) 292, 750, 1083, 1133, 1305
Sau3AI GATC 3 cut(s) 373, 427, 1171
Sau96I GGNCC 6 cut(s) 351, 910, 919, 964, 1229, 1397
SchI GAGTC 5 cut(s) 282, 558, 848, 850, 1206
ScrFI CCNGG 3 cut(s) 512, 524, 535
SduI GDGCHC 2 cut(s) 937, 960
SfaNI GCATC 1 cut(s) 1254
Sfr303I CCGCGG 2 cut(s) 932, 1136
SgrBI CCGCGG 2 cut(s) 932, 1136
SinI GGWCC 4 cut(s) 351, 910, 919, 1229
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
Sse9I AATT 3 cut(s) 797, 1126, 1255
SspMI CTAG 2 cut(s) 327, 1411
StyD4I CCNGG 3 cut(s) 510, 522, 533
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 6 cut(s) 115, 224, 239, 691, 1034, 1207
TaiI ACGT 9 cut(s) 96, 659, 680, 705, 954, 1092, 1166, 1289, 1407
TaqI TCGA 7 cut(s) 90, 666, 857, 1099, 1151, 1158, 1368
TasI AATT 3 cut(s) 797, 1126, 1255
TatI WGTACW 2 cut(s) 174, 432
TauI GCSGC 4 cut(s) 294, 1085, 1135, 1307
TfiI GAWTC 9 cut(s) 161, 204, 323, 402, 446, 988, 1107, 1277, 1354
Tru1I TTAA 2 cut(s) 339, 800
Tru9I TTAA 2 cut(s) 339, 800
TscAI CASTG 3 cut(s) 124, 550, 856
TseFI GTSAC 7 cut(s) 115, 214, 572, 596, 719, 1028, 1063
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 7 cut(s) 115, 214, 572, 596, 719, 1028, 1063
TspDTI ATGAA 2 cut(s) 174, 855
TspGWI ACGGA 6 cut(s) 173, 556, 645, 981, 1238, 1386
TspRI CASTG 3 cut(s) 124, 550, 856
Tth111I GACNNNGTC 2 cut(s) 272, 1207
VpaK11BI GGWCC 4 cut(s) 351, 910, 919, 1229
XapI RAATTY 2 cut(s) 1126, 1255
XbaI TCTAGA 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 2 cut(s) 327, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.