MD00G1127200.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014901
Physical Location & Seq
Forward (+)
26957634 .. 26959277
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1127200.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGGCAGCCGTACGCGTGAAAGAAGCAGTAGTACTATTACTACTACTAACGGTGGCTGCTCTCCTCCTCCTAATTAGCAGCTGCGCCGCCGCCACCATCACCGCCGCCGCCGCCACCACCATCACCAATTCTACAACTACTTCCCCAACGTACCCTGATTTTCAAGAGCTGAACGTGAAAGAAACAATCATGGCGTTTCAAATAAATAGCAGGGCACCTAGGGCTGCATACAACTTCACCAGAAGTACCACCACCGGCAGCAAATTAAATGATGATGATGATAAAGATGAATTAGATCATGAAGAAGAAGAAGATGAAAGAAGCACTAATGGCGGATCCGGAGGAGGAAAATGGAAGGTGAAGGTGGTTCACAGAGATAAGATGTCGTCGCAATATTCAAACAGGAATGGGAATGGCGGCAGCCATACGTCTCATAGGCACCTTTTCCGTGCACGCATGAAAAGGGATGCCAAAAGGGTTGCTAGCCTCACGAGGCACCTCCTCAACTGCTCAAACTCAGAGTTAGAGTCGTTGGATTTTGGGTCGGGGGTGGTGTCGGGTATGGAGCAAGGGAGCGGAGAATACTTCGTGAGGATAGGAGTGGGGAGCCCGCCACGAAACCAATACATGGTGATTGACTCCGGCAGTGACATCGTGTGGGTTCAGTGCCAGCCTTGTACCGAGTGTTACCACCAGTCCGACCCTATCTTTGACCCCGCCCGCTCTGCTTCCTATTCTGGCGTCTCCTGTGCCTCCTCCCTCTGCGGCCGCCTCCAGAACCCCGGTTGTCACTCCGGTCGATGCCGCTACGAGGCCTCCTACGGCGACGGATCCTACACCCAGGGCACGCTTGCCCTGGAAACTCTTACCTTGGGCCGTGCGCTCATCCGAAACGTAGCCATCGGCTGCGGCCACATGAATCGCGGCATGTTTGTGGGAGCCGCTGGTCTCTTGGGCCTTGGAGGCGGGCCCATGTCTTTTGTCGGTCAGCTCGCCGGCCTGACCGGAGGTGCCTTCACTTACTGTTTAGTCAGCCGTGGCGGCCGCTCTAGTGCTACAAGTACTAACGTAGCCGGCGGAGGAGGAGGAGGGTCCCTGGAGTTCGGTCGTGGGGCGATGCCCGTGGGTGCTTCGTGGGTCCCGCTGATCCGGAACCCGCAGGCCCCGAGTTTCTATTTTGTGGGGCTGGCGGGTCTTGGAGTTGGAGGGATGCGGGTACCCATATCCGGAGATGTATTCGGCCAAACCGAGTTGGGGTACGGAGGGGTTGTGATGGACACGGGTACCGCCGTGACGAGGTTCCCTACGTTGGCTTACGAGGCCTTCCGCGATACCTTTCTTCAGCAGACGGCCAGCCTCCCTCGGCTGTCGACGGGAGTGTCCATTTTTGACACATGCTATGACCTGAACGGCTTCGTGTCGGTTCGGGTGCCGACCGTGTCGTTTTATTTTTCGGGTGGGCCCATTCTAACGCTTCCGGCGAGCAATTTTCTGATTCCGGTGGACGACAAAGGCACTTTTTGCTTTGCATTTGCTCCTTCGCCATCCGGACTTTCCATAATAGGCAACATCCAGCAAGAGGGCATCCAAATTTCATTTGATGGAGCCAATGGTTTTGTAGGTTTTGGACCTAATGTCTGCTAA

Protein Analysis

548

Amino Acids

57.62

Weight (kDa)

6.51

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 194 - 246 4.4e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 195 - 349 1.7e-47 Xylanase inhibitor N-terminal
Asp PF00026 271 - 543 2e-06 Eukaryotic aspartyl protease
TAXi_C PF14541 392 - 543 4.9e-28 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20015
fragaria_vesca FvH4_4g37260
malus_domestica MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica Prupe.1G350500_v2.0.a1
pyrus_communis pycom13g00180 pycom16g00070
rosa_chinensis RchiOBHm_Chr4g0447081
rosa_laevigata RLG00000005607
rosa_roxburghii Rroxscaffold_5G00387390
rosa_rugosa Rorug04G0377800
rosa_samantha Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana Rw4G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1378
Acc65I GGTACC 2 cut(s) 1216, 1283
AccB1I GGYRCC 7 cut(s) 214, 438, 495, 1010, 1216, 1283, 1429
AccB7I CCANNNNNTGG 1 cut(s) 628
AccBSI CCGCTC 3 cut(s) 576, 723, 1047
AccI GTMKAC 1 cut(s) 1370
AccII CGCG 3 cut(s) 15, 924, 1329
AccIII TCCGGA 4 cut(s) 338, 1149, 1226, 1547
AclWI GGATC 5 cut(s) 330, 343, 825, 838, 1141
AcoI YGGCCR 5 cut(s) 766, 910, 1042, 1240, 1350
AcsI RAATTY 1 cut(s) 1590
AcuI CTGAAG 1 cut(s) 1325
AcyI GRCGYC 1 cut(s) 741
AfaI GTAC 9 cut(s) 12, 33, 152, 247, 679, 1063, 1218, 1259, 1285
AfiI CCNNNNNNNGG 7 cut(s) 628, 811, 1226, 1254, 1296, 1309, 1579
AflIII ACRYGT 1 cut(s) 13
AgsI TTSAA 3 cut(s) 164, 200, 399
AhdI GACNNNNNGTC 1 cut(s) 1634
AjnI CCWGG 3 cut(s) 840, 855, 1095
AluBI AGCT 3 cut(s) 81, 169, 991
AluI AGCT 3 cut(s) 81, 169, 991
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 3 cut(s) 435, 748, 953
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 5 cut(s) 330, 343, 825, 838, 1141
Ama87I CYCGRG 1 cut(s) 1165
Aor13HI TCCGGA 4 cut(s) 338, 1149, 1226, 1547
ApaI GGGCCC 2 cut(s) 972, 1464
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 8 cut(s) 5, 56, 78, 81, 224, 258, 420, 906
ApoI RAATTY 1 cut(s) 1590
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
Asp700I GAANNNNTTC 1 cut(s) 1412
Asp718I GGTACC 2 cut(s) 1216, 1283
AspA2I CCTAGG 1 cut(s) 218
AspLEI GCGC 2 cut(s) 86, 883
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 5 cut(s) 91, 115, 229, 370, 643
AsuNHI GCTAGC 1 cut(s) 482
AvaI CYCGRG 1 cut(s) 1165
AvaII GGWCC 3 cut(s) 1092, 1138, 1628
AvrII CCTAGG 1 cut(s) 218
BaeGI GKGCMC 5 cut(s) 217, 454, 848, 972, 1464
BaeI ACNNNNGTAYC 4 cut(s) 1267, 1275, 1300, 1308
BamHI GGATCC 2 cut(s) 335, 830
BanI GGYRCC 7 cut(s) 214, 438, 495, 1010, 1216, 1283, 1429
BanII GRGCYC 3 cut(s) 611, 972, 1464
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BauI CACGAG 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 8 cut(s) 17, 43, 68, 90, 211, 270, 432, 893
BccI CCATC 6 cut(s) 104, 128, 908, 1267, 1552, 1595
BceAI ACGGC 6 cut(s) 838, 861, 1020, 1274, 1365, 1426
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 3 cut(s) 842, 857, 1097
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 3 cut(s) 435, 748, 953
BfaI CTAG 3 cut(s) 219, 483, 1050
BglI GCCNNNNNGGC 2 cut(s) 963, 1041
BlnI CCTAGG 1 cut(s) 218
BmcAI AGTACT 2 cut(s) 33, 1063
Bme1390I CCNGG 4 cut(s) 783, 842, 857, 1097
Bme18I GGWCC 3 cut(s) 1092, 1138, 1628
BmeRI GACNNNNNGTC 1 cut(s) 1634
BmeT110I CYCGRG 1 cut(s) 1165
BmrFI CCNGG 4 cut(s) 783, 842, 857, 1097
BmsI GCATC 5 cut(s) 457, 791, 1107, 1200, 1593
BmtI GCTAGC 1 cut(s) 486
BpmI CTGGAG 2 cut(s) 758, 1118
BpuMI CCSGG 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 741
BsaI GGTCTC 1 cut(s) 953
BsaWI WCCGGW 7 cut(s) 338, 794, 1004, 1149, 1226, 1498, 1547
BsaXI ACNNNNNCTCC 4 cut(s) 800, 830, 1254, 1284
Bsc4I CCNNNNNNNGG 7 cut(s) 628, 811, 1226, 1254, 1296, 1309, 1579
Bse118I RCCGGY 3 cut(s) 254, 995, 1073
Bse1I ACTGG 1 cut(s) 694
BseAI TCCGGA 4 cut(s) 338, 1149, 1226, 1547
BseBI CCWGG 3 cut(s) 842, 857, 1097
BseGI GGATG 6 cut(s) 472, 885, 1215, 1544, 1569, 1584
BseLI CCNNNNNNNGG 7 cut(s) 628, 811, 1226, 1254, 1296, 1309, 1579
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 694
BseRI GAGGAG 8 cut(s) 53, 56, 357, 491, 745, 1095, 1098, 1101
BseSI GKGCMC 5 cut(s) 217, 454, 848, 972, 1464
BseX3I CGGCCG 2 cut(s) 766, 1042
BseXI GCAGC 8 cut(s) 17, 43, 68, 90, 211, 270, 432, 893
Bsh1236I CGCG 3 cut(s) 15, 924, 1329
Bsh1285I CGRYCG 5 cut(s) 769, 799, 1045, 1108, 1437
BshNI GGYRCC 7 cut(s) 214, 438, 495, 1010, 1216, 1283, 1429
BsiEI CGRYCG 5 cut(s) 769, 799, 1045, 1108, 1437
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 1165
BsiWI CGTACG 1 cut(s) 10
BslFI GGGAC 2 cut(s) 1078, 1124
BslI CCNNNNNNNGG 7 cut(s) 628, 811, 1226, 1254, 1296, 1309, 1579
BsmAI GTCTC 3 cut(s) 435, 748, 953
BsmBI CGTCTC 2 cut(s) 435, 748
BsmFI GGGAC 2 cut(s) 1078, 1124
Bso31I GGTCTC 1 cut(s) 953
BsoBI CYCGRG 1 cut(s) 1165
Bsp120I GGGCCC 2 cut(s) 968, 1460
Bsp1286I GDGCHC 6 cut(s) 217, 454, 611, 848, 972, 1464
Bsp13I TCCGGA 4 cut(s) 338, 1149, 1226, 1547
Bsp143I GATC 4 cut(s) 295, 335, 830, 1146
BspCNI CTCAG 1 cut(s) 530
BspEI TCCGGA 4 cut(s) 338, 1149, 1226, 1547
BspFNI CGCG 3 cut(s) 15, 924, 1329
BspHI TCATGA 1 cut(s) 298
BspOI GCTAGC 1 cut(s) 486
BspPI GGATC 5 cut(s) 330, 343, 825, 838, 1141
BspT107I GGYRCC 7 cut(s) 214, 438, 495, 1010, 1216, 1283, 1429
BspTNI GGTCTC 1 cut(s) 953
BsrBI CCGCTC 3 cut(s) 576, 723, 1047
BsrFI RCCGGY 3 cut(s) 254, 995, 1073
BsrI ACTGG 1 cut(s) 694
BssAI RCCGGY 3 cut(s) 254, 995, 1073
BssMI GATC 4 cut(s) 295, 335, 830, 1146
BssNI GRCGYC 1 cut(s) 741
BssSI CACGAG 1 cut(s) 490
BssT1I CCWWGG 3 cut(s) 218, 870, 958
Bst2BI CACGAG 1 cut(s) 490
Bst2UI CCWGG 3 cut(s) 842, 857, 1097
Bst4CI ACNGT 3 cut(s) 52, 1025, 1438
BstACI GRCGYC 1 cut(s) 741
BstAPI GCANNNNNTGC 1 cut(s) 1521
BstDEI CTNAG 1 cut(s) 517
BstDSI CCRYGG 2 cut(s) 1036, 1122
BstF5I GGATG 6 cut(s) 472, 885, 1215, 1544, 1569, 1584
BstFNI CGCG 3 cut(s) 15, 924, 1329
BstHHI GCGC 2 cut(s) 86, 883
BstKTI GATC 4 cut(s) 298, 338, 833, 1149
BstMAI GTCTC 3 cut(s) 435, 748, 953
BstMBI GATC 4 cut(s) 295, 335, 830, 1146
BstMCI CGRYCG 5 cut(s) 769, 799, 1045, 1108, 1437
BstMWI GCNNNNNNNGC 8 cut(s) 110, 221, 330, 725, 963, 1041, 1319, 1521
BstNI CCWGG 3 cut(s) 842, 857, 1097
BstNSI RCATGY 2 cut(s) 931, 1398
BstSCI CCNGG 4 cut(s) 781, 840, 855, 1095
BstSLI GKGCMC 5 cut(s) 217, 454, 848, 972, 1464
BstUI CGCG 3 cut(s) 15, 924, 1329
BstV1I GCAGC 8 cut(s) 17, 43, 68, 90, 211, 270, 432, 893
BstX2I RGATCY 2 cut(s) 335, 830
BstYI RGATCY 2 cut(s) 335, 830
BstZI CGGCCG 2 cut(s) 766, 1042
BtgI CCRYGG 2 cut(s) 1036, 1122
BtgZI GCGATG 1 cut(s) 1130
BtsCI GGATG 6 cut(s) 472, 885, 1215, 1544, 1569, 1584
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 652, 671
CciI TCATGA 1 cut(s) 298
CciNI GCGGCCGC 2 cut(s) 766, 1042
CfoI GCGC 2 cut(s) 86, 883
Cfr10I RCCGGY 3 cut(s) 254, 995, 1073
CseI GACGC 1 cut(s) 730
Csp6I GTAC 9 cut(s) 11, 32, 151, 246, 678, 1062, 1217, 1258, 1284
CviAII CATG 8 cut(s) 190, 299, 457, 628, 916, 928, 973, 1395
CviQI GTAC 9 cut(s) 11, 32, 151, 246, 678, 1062, 1217, 1258, 1284
DdeI CTNAG 1 cut(s) 517
DpnI GATC 4 cut(s) 297, 337, 832, 1148
DpnII GATC 4 cut(s) 295, 335, 830, 1146
DrdI GACNNNNNNGTC 1 cut(s) 1378
DriI GACNNNNNGTC 1 cut(s) 1634
DseDI GACNNNNNNGTC 1 cut(s) 1378
EaeI YGGCCR 5 cut(s) 766, 910, 1042, 1240, 1350
EagI CGGCCG 2 cut(s) 766, 1042
Eam1105I GACNNNNNGTC 1 cut(s) 1634
EciI GGCGGA 2 cut(s) 348, 1092
EclXI CGGCCG 2 cut(s) 766, 1042
Eco130I CCWWGG 3 cut(s) 218, 870, 958
Eco147I AGGCCT 2 cut(s) 815, 1322
Eco24I GRGCYC 3 cut(s) 611, 972, 1464
Eco31I GGTCTC 1 cut(s) 953
Eco47I GGWCC 3 cut(s) 1092, 1138, 1628
Eco52I CGGCCG 2 cut(s) 766, 1042
Eco57I CTGAAG 1 cut(s) 1325
Eco88I CYCGRG 1 cut(s) 1165
EcoO109I RGGNCCY 3 cut(s) 1092, 1138, 1162
EcoRII CCWGG 3 cut(s) 840, 855, 1095
EcoT14I CCWWGG 3 cut(s) 218, 870, 958
EcoT38I GRGCYC 3 cut(s) 611, 972, 1464
ErhI CCWWGG 3 cut(s) 218, 870, 958
Esp3I CGTCTC 2 cut(s) 435, 748
FaeI CATG 8 cut(s) 193, 302, 460, 631, 919, 931, 976, 1398
FaqI GGGAC 2 cut(s) 1078, 1124
FatI CATG 8 cut(s) 189, 298, 456, 627, 915, 927, 972, 1394
FauI CCCGC 8 cut(s) 618, 724, 728, 959, 1149, 1164, 1183, 1206
FblI GTMKAC 1 cut(s) 1370
FokI GGATG 6 cut(s) 479, 872, 1222, 1531, 1556, 1571
FriOI GRGCYC 3 cut(s) 611, 972, 1464
FspBI CTAG 3 cut(s) 219, 483, 1050
GlaI GCGC 2 cut(s) 85, 882
GsuI CTGGAG 2 cut(s) 758, 1118
HgaI GACGC 1 cut(s) 730
HhaI GCGC 2 cut(s) 86, 883
Hin1I GRCGYC 1 cut(s) 741
Hin1II CATG 8 cut(s) 193, 302, 460, 631, 919, 931, 976, 1398
Hin6I GCGC 2 cut(s) 84, 881
HinP1I GCGC 2 cut(s) 84, 881
HincII GTYRAC 1 cut(s) 1371
HindII GTYRAC 1 cut(s) 1371
HinfI GANTC 4 cut(s) 527, 638, 919, 1495
HphI GGTGA 5 cut(s) 91, 115, 229, 370, 643
Hpy166II GTNNAC 4 cut(s) 370, 452, 1371, 1504
Hpy188I TCNGA 4 cut(s) 520, 700, 890, 1494
Hpy188III TCNNGA 9 cut(s) 164, 299, 339, 490, 589, 775, 1150, 1227, 1548
Hpy8I GTNNAC 4 cut(s) 370, 452, 1371, 1504
Hpy99I CGWCG 3 cut(s) 391, 830, 1375
HpyAV CCTTC 5 cut(s) 349, 355, 1024, 1333, 1548
HpyCH4III ACNGT 3 cut(s) 52, 1025, 1438
HpyCH4IV ACGT 6 cut(s) 149, 174, 428, 894, 1068, 1307
HpyCH4V TGCA 3 cut(s) 227, 452, 1529
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 221, 330, 725, 963, 1041, 1319, 1521
HpyF3I CTNAG 1 cut(s) 517
HpySE526I ACGT 6 cut(s) 149, 174, 428, 894, 1068, 1307
Hsp92I GRCGYC 1 cut(s) 741
Hsp92II CATG 8 cut(s) 193, 302, 460, 631, 919, 931, 976, 1398
HspAI GCGC 2 cut(s) 84, 881
KflI GGGWCCC 2 cut(s) 1092, 1138
Kpn2I TCCGGA 4 cut(s) 338, 1149, 1226, 1547
KpnI GGTACC 2 cut(s) 1220, 1287
KroI GCCGGC 2 cut(s) 995, 1073
KroNI GCCGGC 2 cut(s) 997, 1075
Kzo9I GATC 4 cut(s) 295, 335, 830, 1146
LmnI GCTCC 6 cut(s) 565, 573, 606, 938, 1540, 1604
Lsp1109I GCAGC 8 cut(s) 17, 43, 68, 90, 211, 270, 432, 893
LweI GCATC 5 cut(s) 457, 791, 1107, 1200, 1593
MaeI CTAG 3 cut(s) 219, 483, 1050
MaeII ACGT 6 cut(s) 149, 174, 428, 894, 1068, 1307
MaeIII GTNAC 4 cut(s) 647, 686, 788, 1291
MalI GATC 4 cut(s) 297, 337, 832, 1148
MbiI CCGCTC 3 cut(s) 576, 723, 1047
MboI GATC 4 cut(s) 295, 335, 830, 1146
MboII GAAGA 5 cut(s) 314, 317, 320, 323, 1331
MflI RGATCY 2 cut(s) 335, 830
MhlI GDGCHC 6 cut(s) 217, 454, 611, 848, 972, 1464
MluCI AATT 6 cut(s) 72, 127, 263, 290, 1486, 1590
MluI ACGCGT 1 cut(s) 13
MlyI GAGTC 2 cut(s) 536, 632
MmeI TCCRAC 3 cut(s) 513, 723, 1183
MroI TCCGGA 4 cut(s) 338, 1149, 1226, 1547
MroNI GCCGGC 2 cut(s) 995, 1073
MroXI GAANNNNTTC 1 cut(s) 1412
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 3 cut(s) 81, 944, 1144
MspR9I CCNGG 4 cut(s) 783, 842, 857, 1097
MvaI CCWGG 3 cut(s) 842, 857, 1097
MvnI CGCG 3 cut(s) 15, 924, 1329
MwoI GCNNNNNNNGC 8 cut(s) 110, 221, 330, 725, 963, 1041, 1319, 1521
NaeI GCCGGC 2 cut(s) 997, 1075
NciI CCSGG 1 cut(s) 783
NdeII GATC 4 cut(s) 295, 335, 830, 1146
NgoMIV GCCGGC 2 cut(s) 995, 1073
NheI GCTAGC 1 cut(s) 482
NlaIII CATG 8 cut(s) 193, 302, 460, 631, 919, 931, 976, 1398
NmeAIII GCCGAG 1 cut(s) 1342
NmuCI GTSAC 3 cut(s) 647, 788, 1291
NotI GCGGCCGC 2 cut(s) 766, 1042
NspI RCATGY 2 cut(s) 931, 1398
PagI TCATGA 1 cut(s) 298
PasI CCCWGGG 1 cut(s) 841
PceI AGGCCT 2 cut(s) 815, 1322
PcsI WCGNNNNNNNCGW 2 cut(s) 1245, 1478
PdiI GCCGGC 2 cut(s) 997, 1075
PdmI GAANNNNTTC 1 cut(s) 1412
PfeI GAWTC 2 cut(s) 919, 1495
Pfl23II CGTACG 1 cut(s) 10
PflFI GACNNNGTC 1 cut(s) 1438
PflMI CCANNNNNTGG 1 cut(s) 628
PleI GAGTC 2 cut(s) 535, 632
PpsI GAGTC 2 cut(s) 535, 632
PpuMI RGGWCCY 2 cut(s) 1092, 1138
Psp5II RGGWCCY 2 cut(s) 1092, 1138
Psp6I CCWGG 3 cut(s) 840, 855, 1095
PspGI CCWGG 3 cut(s) 840, 855, 1095
PspLI CGTACG 1 cut(s) 10
PspOMI GGGCCC 2 cut(s) 968, 1460
PspPPI RGGWCCY 2 cut(s) 1092, 1138
PsuI RGATCY 2 cut(s) 335, 830
PsyI GACNNNGTC 1 cut(s) 1438
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 9 cut(s) 12, 33, 152, 247, 679, 1063, 1218, 1259, 1285
RsaNI GTAC 9 cut(s) 11, 32, 151, 246, 678, 1062, 1217, 1258, 1284
RseI CAYNNNNRTG 1 cut(s) 932
SalI GTCGAC 1 cut(s) 1369
SaqAI TTAA 1 cut(s) 266
Sau3AI GATC 4 cut(s) 295, 335, 830, 1146
ScaI AGTACT 2 cut(s) 33, 1063
SchI GAGTC 2 cut(s) 536, 632
ScrFI CCNGG 4 cut(s) 783, 842, 857, 1097
SduI GDGCHC 6 cut(s) 217, 454, 611, 848, 972, 1464
SfaNI GCATC 5 cut(s) 457, 791, 1107, 1200, 1593
SinI GGWCC 3 cut(s) 1092, 1138, 1628
SmiMI CAYNNNNRTG 1 cut(s) 932
Sse9I AATT 6 cut(s) 72, 127, 263, 290, 1486, 1590
SseBI AGGCCT 2 cut(s) 815, 1322
SspI AATATT 1 cut(s) 395
SspMI CTAG 3 cut(s) 219, 483, 1050
StuI AGGCCT 2 cut(s) 815, 1322
StyD4I CCNGG 4 cut(s) 781, 840, 855, 1095
StyI CCWWGG 3 cut(s) 218, 870, 958
TaaI ACNGT 3 cut(s) 52, 1025, 1438
TaiI ACGT 6 cut(s) 152, 177, 431, 897, 1071, 1310
TaqI TCGA 2 cut(s) 799, 1370
TaqII GACCGA 2 cut(s) 974, 1094
TasI AATT 6 cut(s) 72, 127, 263, 290, 1486, 1590
TatI WGTACW 2 cut(s) 31, 1061
TfiI GAWTC 2 cut(s) 919, 1495
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TscAI CASTG 2 cut(s) 652, 671
TseFI GTSAC 3 cut(s) 647, 788, 1291
TseI GCWGC 8 cut(s) 5, 56, 78, 81, 224, 258, 420, 906
Tsp45I GTSAC 3 cut(s) 647, 788, 1291
TspDTI ATGAA 6 cut(s) 303, 315, 330, 473, 932, 1584
TspGWI ACGGA 3 cut(s) 437, 843, 1275
TspRI CASTG 2 cut(s) 652, 671
Tth111I GACNNNGTC 1 cut(s) 1438
Van91I CCANNNNNTGG 1 cut(s) 628
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 3 cut(s) 1092, 1138, 1628
XapI RAATTY 1 cut(s) 1590
XceI RCATGY 2 cut(s) 931, 1398
XmaJI CCTAGG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 1370
XmnI GAANNNNTTC 1 cut(s) 1412
XspI CTAG 3 cut(s) 219, 483, 1050
ZrmI AGTACT 2 cut(s) 33, 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.