Rh4BG448100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014901
Physical Location & Seq
Forward (+)
58632748 .. 58634689
1942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG448100.1

Sequence Viewer

Length: 1440 bp
ATGGAGGGGATGCTATTACTCTGCGTGGCTCTAGTCATAACAAGTTTCGGTATTAGCGGCACCGCCACCACTTACCCCGATTTCCAACAGTTGCACGTCAAACAAACAATCGCTTCTGCTTCTGCTTCTGCTGCCACCAAGCTATACAAGGAAGAAGATGATGATGGTGGTGGTAGCGAAAGCGGAGGAGCAAGATGGAAGGTGAAGGTGGTTCATAGAGATAAGATATTCCCACAAGCACCATACAACAAGAGCAGCAATTCACATCATCTGTTTCATGCACGCATCAACAGAGACGTGAAAAGGGTTGATAGCCTTATGCGTCGCCTCGGTAATAGTAAACTAGTCGTCAATTCCGCCACGGACTTTGGATCGGAGGTGGTTTCGGGGATGGCGCAAGGCAGCGGTGAATACTTTGTTAGGATCGGCGTTGGCAGCCCTCCCAGAAGCCAATACGTGGTCATTGACTCCGGCAGCGATATTGTGTGGGTCCAGTGTCAACCTTGCAGCCAGTGTTATCACCAGTCCGACCCGATCTTCGACCCGGCACACTCTGCTTCTTACTCCGGCGTGTCTTGCACCTCCACCGTGTGTGGCCGCCTCCAGAACCCGGGCTGCCACGCAGGTCGGTGCCGGTACGAGGCTTCCTACGGCGACGGCTCCTACACGCAGGGCACTCTCGCCCTCGAAACAGTCACCTTCGGCCGCGCTCTCATCCGCAACGTGGCCATCGGCTGCGGCCACATGAACCGGGGCATGTTCGTCGGAGCCGCAGGTCTTTTGGGCCTCGGGAGTGGGCCCATGTCATTCGTGGGCCAGCTGGGCGGCCAGACTGGCGGTGCATTTTCCTACTGTTTGGTCAGCCGGGGTGACGGTGACGCCACCACCGGCTCACTCGAGTTCGGCCGTACCGCGATGCCCGTGGGTGCTGCGTGGGTCCCTTTGATCCGGAACCAACGGGCCCCGAGTTTCTACTACGTTGGCCTTGCGGGCCTTGGTGTCGGAGGCATGCGGGTCCCAATATCCGAAGACGTGTTCCGGCAGACGGAGCTCGGTGACGGGGGCGTTGTTATGGACACCGGAACCGCCGTCACCAGGTTCCCGACGTTGGCTTATCAGGCATTCCGCGACGCGTTTCTTCAGCAAACCGCAAACCTCCCTCGGGTCTCCGGAGTCTCCATCTTCGACACGTGTTATGACCTGAACGGGTTTGTGTCGGTTCGGGTACCGACCGTTTCCTTTTATTTCTCGGGCGGGCCCATCCTGACCCTTCCGGCAAGGAACTTTCTGATTCCGGTGGATGACAAGGGTACGTTTTGTTTGGCATTTGCTCCTTCCGCTGCAGGGCTTTCCATAATAGGGAATATTCAGCAAGAGGGCATTCAAATCTCATTTGATGGAGCTAATGGTTTTGTAGGATTTGGACCCAATGTCTGCTAG

Protein Analysis

479

Amino Acids

50.67

Weight (kDa)

7.07

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 137 - 189 1.7e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 138 - 302 1.1e-47 Xylanase inhibitor N-terminal
Asp PF00026 214 - 475 2.5e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 325 - 475 3.4e-31 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20015
fragaria_vesca FvH4_4g37260
malus_domestica MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica Prupe.1G350500_v2.0.a1
pyrus_communis pycom13g00180 pycom16g00070
rosa_chinensis RchiOBHm_Chr4g0447081
rosa_laevigata RLG00000005607
rosa_roxburghii Rroxscaffold_5G00387390
rosa_rugosa Rorug04G0377800
rosa_samantha Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana Rw4G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 614, 764
Acc65I GGTACC 1 cut(s) 1225
AccB1I GGYRCC 3 cut(s) 59, 630, 1225
AccB7I CCANNNNNTGG 1 cut(s) 457
AccII CGCG 4 cut(s) 708, 914, 1128, 1133
AccIII TCCGGA 2 cut(s) 948, 1169
AclWI GGATC 3 cut(s) 379, 431, 940
AcoI YGGCCR 6 cut(s) 595, 703, 726, 739, 826, 904
AcuI CTGAAG 1 cut(s) 1124
AcvI CACGTG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 879
AdeI CACNNNGTG 1 cut(s) 591
AfaI GTAC 4 cut(s) 638, 910, 1227, 1312
AflIII ACRYGT 4 cut(s) 1032, 1131, 1188, 1190
AgsI TTSAA 1 cut(s) 1385
AhdI GACNNNNNGTC 1 cut(s) 1430
AhlI ACTAGT 1 cut(s) 343
AjiI CACGTC 3 cut(s) 97, 298, 1033
AjnI CCWGG 1 cut(s) 1094
AluBI AGCT 4 cut(s) 142, 820, 1051, 1403
AluI AGCT 4 cut(s) 142, 820, 1051, 1403
Alw21I GWGCWC 1 cut(s) 1053
Alw26I GTCTC 3 cut(s) 288, 1171, 1180
AlwI GGATC 3 cut(s) 379, 431, 940
Ama87I CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
Aor13HI TCCGGA 2 cut(s) 948, 1169
ApaI GGGCCC 3 cut(s) 801, 964, 1260
ArsI GACNNNNNNTTYG 2 cut(s) 521, 553
Asp718I GGTACC 1 cut(s) 1225
AspLEI GCGC 2 cut(s) 397, 710
AsuC2I CCSGG 5 cut(s) 545, 611, 612, 752, 866
AsuHPI GGTGA 8 cut(s) 214, 419, 512, 688, 881, 887, 1067, 1084
AvaI CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
AvaII GGWCC 4 cut(s) 490, 937, 1015, 1424
BaeGI GKGCMC 4 cut(s) 677, 801, 964, 1260
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 3 cut(s) 59, 630, 1225
BanII GRGCYC 4 cut(s) 801, 964, 1053, 1260
BbrPI CACGTG 1 cut(s) 1191
BbsI GAAGAC 1 cut(s) 1035
Bbv12I GWGCWC 1 cut(s) 1053
BccI CCATC 7 cut(s) 158, 189, 385, 737, 1187, 1268, 1391
BceAI ACGGC 4 cut(s) 667, 673, 891, 1073
BcgI CGANNNNNNTGC 2 cut(s) 745, 779
BciT130I CCWGG 1 cut(s) 1096
BcnI CCSGG 5 cut(s) 545, 611, 612, 752, 866
BcoDI GTCTC 3 cut(s) 288, 1171, 1180
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 3 cut(s) 32, 344, 1438
BfmI CTRYAG 1 cut(s) 1341
BfuAI ACCTGC 2 cut(s) 614, 764
BglI GCCNNNNNGGC 3 cut(s) 822, 834, 990
Bme1390I CCNGG 6 cut(s) 545, 611, 612, 752, 866, 1096
Bme18I GGWCC 4 cut(s) 490, 937, 1015, 1424
BmeRI GACNNNNNGTC 1 cut(s) 1430
BmeT110I CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
BmgBI CACGTC 3 cut(s) 97, 298, 1033
BmrFI CCNGG 6 cut(s) 545, 611, 612, 752, 866, 1096
BmsI GCATC 2 cut(s) 294, 906
BpiI GAAGAC 1 cut(s) 1035
BpmI CTGGAG 1 cut(s) 587
BpuMI CCSGG 5 cut(s) 545, 611, 612, 752, 866
BsaAI YACGTR 2 cut(s) 457, 1191
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 1171
BsaJI CCNNGG 9 cut(s) 328, 360, 610, 751, 787, 865, 921, 994, 1160
BsaWI WCCGGW 4 cut(s) 948, 1079, 1169, 1294
Bse118I RCCGGY 2 cut(s) 633, 887
Bse1I ACTGG 4 cut(s) 493, 511, 523, 838
BseAI TCCGGA 2 cut(s) 948, 1169
BseBI CCWGG 1 cut(s) 1096
BseDI CCNNGG 9 cut(s) 328, 360, 610, 751, 787, 865, 921, 994, 1160
BseGI GGATG 5 cut(s) 15, 396, 714, 1260, 1306
BseNI ACTGG 4 cut(s) 493, 511, 523, 838
BseRI GAGGAG 1 cut(s) 201
BseSI GKGCMC 4 cut(s) 677, 801, 964, 1260
BseX3I CGGCCG 2 cut(s) 703, 904
BseYI CCCAGC 1 cut(s) 820
Bsh1236I CGCG 4 cut(s) 708, 914, 1128, 1133
Bsh1285I CGRYCG 3 cut(s) 706, 907, 1233
BshNI GGYRCC 3 cut(s) 59, 630, 1225
BsiEI CGRYCG 3 cut(s) 706, 907, 1233
BsiHKAI GWGCWC 1 cut(s) 1053
BsiHKCI CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
BslFI GGGAC 2 cut(s) 923, 1001
BsmAI GTCTC 3 cut(s) 288, 1171, 1180
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 2 cut(s) 923, 1001
BsmI GAATGC 2 cut(s) 1121, 1380
Bso31I GGTCTC 1 cut(s) 1171
BsoBI CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
Bsp120I GGGCCC 3 cut(s) 797, 960, 1256
Bsp1286I GDGCHC 5 cut(s) 677, 801, 964, 1053, 1260
Bsp13I TCCGGA 2 cut(s) 948, 1169
Bsp143I GATC 4 cut(s) 371, 423, 534, 945
BspEI TCCGGA 2 cut(s) 948, 1169
BspFNI CGCG 4 cut(s) 708, 914, 1128, 1133
BspMAI CTGCAG 1 cut(s) 1345
BspMI ACCTGC 2 cut(s) 614, 764
BspPI GGATC 3 cut(s) 379, 431, 940
BspT107I GGYRCC 3 cut(s) 59, 630, 1225
BspTNI GGTCTC 1 cut(s) 1171
BsrFI RCCGGY 2 cut(s) 633, 887
BsrI ACTGG 4 cut(s) 493, 511, 523, 838
BssAI RCCGGY 2 cut(s) 633, 887
BssECI CCNNGG 9 cut(s) 328, 360, 610, 751, 787, 865, 921, 994, 1160
BssMI GATC 4 cut(s) 371, 423, 534, 945
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 1 cut(s) 994
Bst2UI CCWGG 1 cut(s) 1096
Bst4CI ACNGT 6 cut(s) 90, 589, 694, 854, 875, 1234
BstACI GRCGYC 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 554
BstBAI YACGTR 2 cut(s) 457, 1191
BstC8I GCNNGC 5 cut(s) 283, 818, 991, 1010, 1256
BstDSI CCRYGG 2 cut(s) 360, 921
BstF5I GGATG 5 cut(s) 15, 396, 714, 1260, 1306
BstFNI CGCG 4 cut(s) 708, 914, 1128, 1133
BstHHI GCGC 2 cut(s) 397, 710
BstKTI GATC 4 cut(s) 374, 426, 537, 948
BstMAI GTCTC 3 cut(s) 288, 1171, 1180
BstMBI GATC 4 cut(s) 371, 423, 534, 945
BstMCI CGRYCG 3 cut(s) 706, 907, 1233
BstNI CCWGG 1 cut(s) 1096
BstNSI RCATGY 2 cut(s) 760, 1012
BstSCI CCNGG 6 cut(s) 543, 609, 610, 750, 864, 1094
BstSFI CTRYAG 1 cut(s) 1341
BstSLI GKGCMC 4 cut(s) 677, 801, 964, 1260
BstUI CGCG 4 cut(s) 708, 914, 1128, 1133
BstV2I GAAGAC 1 cut(s) 1035
BstZI CGGCCG 2 cut(s) 703, 904
BtgI CCRYGG 2 cut(s) 360, 921
BtgZI GCGATG 1 cut(s) 929
BtrI CACGTC 3 cut(s) 97, 298, 1033
BtsCI GGATG 5 cut(s) 15, 396, 714, 1260, 1306
BtsIMutI CAGTG 2 cut(s) 500, 518
BveI ACCTGC 2 cut(s) 614, 764
Cac8I GCNNGC 5 cut(s) 283, 818, 991, 1010, 1256
CfoI GCGC 2 cut(s) 397, 710
Cfr10I RCCGGY 2 cut(s) 633, 887
Cfr9I CCCGGG 1 cut(s) 610
CseI GACGC 3 cut(s) 311, 887, 1139
CsiI ACCWGGT 1 cut(s) 1094
Csp6I GTAC 4 cut(s) 637, 909, 1226, 1311
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 5 cut(s) 278, 745, 757, 802, 1009
CviQI GTAC 4 cut(s) 637, 909, 1226, 1311
DpnI GATC 4 cut(s) 373, 425, 536, 947
DpnII GATC 4 cut(s) 371, 423, 534, 945
DraIII CACNNNGTG 1 cut(s) 591
DriI GACNNNNNGTC 1 cut(s) 1430
EaeI YGGCCR 6 cut(s) 595, 703, 726, 739, 826, 904
EagI CGGCCG 2 cut(s) 703, 904
Eam1105I GACNNNNNGTC 1 cut(s) 1430
EciI GGCGGA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 1051
EclXI CGGCCG 2 cut(s) 703, 904
Eco130I CCWWGG 1 cut(s) 994
Eco24I GRGCYC 4 cut(s) 801, 964, 1053, 1260
Eco31I GGTCTC 1 cut(s) 1171
Eco47I GGWCC 4 cut(s) 490, 937, 1015, 1424
Eco52I CGGCCG 2 cut(s) 703, 904
Eco53kI GAGCTC 1 cut(s) 1051
Eco57I CTGAAG 1 cut(s) 1124
Eco72I CACGTG 1 cut(s) 1191
Eco88I CYCGRG 6 cut(s) 610, 788, 896, 964, 1161, 1249
EcoICRI GAGCTC 1 cut(s) 1051
EcoO109I RGGNCCY 3 cut(s) 937, 961, 1015
EcoRII CCWGG 1 cut(s) 1094
EcoT14I CCWWGG 1 cut(s) 994
EcoT38I GRGCYC 4 cut(s) 801, 964, 1053, 1260
ErhI CCWWGG 1 cut(s) 994
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 5 cut(s) 281, 748, 760, 805, 1012
FaqI GGGAC 2 cut(s) 923, 1001
FatI CATG 5 cut(s) 277, 744, 756, 801, 1008
FauI CCCGC 3 cut(s) 982, 1005, 1247
FokI GGATG 5 cut(s) 22, 403, 701, 1247, 1313
FriOI GRGCYC 4 cut(s) 801, 964, 1053, 1260
FspBI CTAG 3 cut(s) 32, 344, 1438
GlaI GCGC 2 cut(s) 396, 709
GsaI CCCAGC 1 cut(s) 824
GsuI CTGGAG 1 cut(s) 587
HgaI GACGC 3 cut(s) 311, 887, 1139
HhaI GCGC 2 cut(s) 397, 710
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 5 cut(s) 281, 748, 760, 805, 1012
Hin6I GCGC 2 cut(s) 395, 708
HinP1I GCGC 2 cut(s) 395, 708
HincII GTYRAC 1 cut(s) 500
HindII GTYRAC 1 cut(s) 500
HinfI GANTC 3 cut(s) 467, 1173, 1291
HphI GGTGA 8 cut(s) 214, 419, 512, 688, 881, 887, 1067, 1084
Hpy166II GTNNAC 2 cut(s) 341, 500
Hpy188I TCNGA 6 cut(s) 376, 529, 767, 1004, 1027, 1290
Hpy188III TCNNGA 6 cut(s) 604, 790, 949, 1102, 1170, 1264
Hpy8I GTNNAC 2 cut(s) 341, 500
Hpy99I CGWCG 5 cut(s) 327, 659, 767, 1108, 1133
HpyAV CCTTC 5 cut(s) 193, 199, 709, 1280, 1344
HpyCH4III ACNGT 6 cut(s) 90, 589, 694, 854, 875, 1234
HpyCH4IV ACGT 9 cut(s) 96, 297, 456, 723, 978, 1032, 1106, 1190, 1313
HpyCH4V TGCA 6 cut(s) 94, 281, 507, 579, 842, 1343
HpySE526I ACGT 9 cut(s) 96, 297, 456, 723, 978, 1032, 1106, 1190, 1313
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 5 cut(s) 281, 748, 760, 805, 1012
HspAI GCGC 2 cut(s) 395, 708
KflI GGGWCCC 2 cut(s) 937, 1015
Kpn2I TCCGGA 2 cut(s) 948, 1169
KpnI GGTACC 1 cut(s) 1229
Kzo9I GATC 4 cut(s) 371, 423, 534, 945
LmnI GCTCC 6 cut(s) 188, 665, 767, 1048, 1336, 1400
LweI GCATC 2 cut(s) 294, 906
MabI ACCWGGT 1 cut(s) 1094
MaeI CTAG 3 cut(s) 32, 344, 1438
MaeII ACGT 9 cut(s) 96, 297, 456, 723, 978, 1032, 1106, 1190, 1313
MaeIII GTNAC 5 cut(s) 694, 869, 875, 1055, 1090
MalI GATC 4 cut(s) 373, 425, 536, 947
MboI GATC 4 cut(s) 371, 423, 534, 945
MboII GAAGA 6 cut(s) 164, 167, 529, 1040, 1130, 1174
MhlI GDGCHC 5 cut(s) 677, 801, 964, 1053, 1260
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 2 cut(s) 259, 352
MluI ACGCGT 1 cut(s) 1131
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 2 cut(s) 461, 1182
MmeI TCCRAC 4 cut(s) 109, 552, 745, 982
Mox20I TGGCCA 1 cut(s) 728
MroI TCCGGA 2 cut(s) 948, 1169
MscI TGGCCA 1 cut(s) 728
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 3 cut(s) 405, 820, 1340
MspR9I CCNGG 6 cut(s) 545, 611, 612, 752, 866, 1096
Mva1269I GAATGC 2 cut(s) 1121, 1380
MvaI CCWGG 1 cut(s) 1096
MvnI CGCG 4 cut(s) 708, 914, 1128, 1133
NciI CCSGG 5 cut(s) 545, 611, 612, 752, 866
NdeII GATC 4 cut(s) 371, 423, 534, 945
NlaIII CATG 5 cut(s) 281, 748, 760, 805, 1012
NmuCI GTSAC 5 cut(s) 694, 869, 875, 1055, 1090
NspI RCATGY 2 cut(s) 760, 1012
PaeI GCATGC 1 cut(s) 1012
PaeR7I CTCGAG 1 cut(s) 896
PctI GAATGC 2 cut(s) 1121, 1380
PfeI GAWTC 1 cut(s) 1291
PflMI CCANNNNNTGG 1 cut(s) 457
PleI GAGTC 2 cut(s) 461, 1181
PmaCI CACGTG 1 cut(s) 1191
PmlI CACGTG 1 cut(s) 1191
PpsI GAGTC 2 cut(s) 461, 1181
Ppu21I YACGTR 2 cut(s) 457, 1191
PpuMI RGGWCCY 2 cut(s) 937, 1015
Psp124BI GAGCTC 1 cut(s) 1053
Psp5II RGGWCCY 2 cut(s) 937, 1015
Psp6I CCWGG 1 cut(s) 1094
PspCI CACGTG 1 cut(s) 1191
PspFI CCCAGC 1 cut(s) 820
PspGI CCWGG 1 cut(s) 1094
PspOMI GGGCCC 3 cut(s) 797, 960, 1256
PspPPI RGGWCCY 2 cut(s) 937, 1015
PspXI VCTCGAGB 1 cut(s) 896
PstI CTGCAG 1 cut(s) 1345
PvuII CAGCTG 1 cut(s) 820
RsaI GTAC 4 cut(s) 638, 910, 1227, 1312
RsaNI GTAC 4 cut(s) 637, 909, 1226, 1311
SacI GAGCTC 1 cut(s) 1053
Sau3AI GATC 4 cut(s) 371, 423, 534, 945
SchI GAGTC 2 cut(s) 461, 1182
ScrFI CCNGG 6 cut(s) 545, 611, 612, 752, 866, 1096
SduI GDGCHC 5 cut(s) 677, 801, 964, 1053, 1260
SexAI ACCWGGT 1 cut(s) 1094
SfaNI GCATC 2 cut(s) 294, 906
SfcI CTRYAG 1 cut(s) 1341
SfiI GGCCNNNNNGGCC 1 cut(s) 990
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 4 cut(s) 490, 937, 1015, 1424
SlaI CTCGAG 1 cut(s) 896
SmaI CCCGGG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SpeI ACTAGT 1 cut(s) 343
SphI GCATGC 1 cut(s) 1012
Sse9I AATT 2 cut(s) 259, 352
SspI AATATT 1 cut(s) 1366
SspMI CTAG 3 cut(s) 32, 344, 1438
SstI GAGCTC 1 cut(s) 1053
StyD4I CCNGG 6 cut(s) 543, 609, 610, 750, 864, 1094
StyI CCWWGG 1 cut(s) 994
TaaI ACNGT 6 cut(s) 90, 589, 694, 854, 875, 1234
TaiI ACGT 9 cut(s) 99, 300, 459, 726, 981, 1035, 1109, 1193, 1316
TaqI TCGA 4 cut(s) 540, 687, 897, 1185
TasI AATT 2 cut(s) 259, 352
TauI GCSGC 6 cut(s) 60, 600, 708, 741, 773, 828
TfiI GAWTC 1 cut(s) 1291
TscAI CASTG 2 cut(s) 500, 518
TseFI GTSAC 5 cut(s) 694, 869, 875, 1055, 1090
Tsp45I GTSAC 5 cut(s) 694, 869, 875, 1055, 1090
TspDTI ATGAA 3 cut(s) 203, 266, 761
TspGWI ACGGA 2 cut(s) 377, 1061
TspMI CCCGGG 1 cut(s) 610
TspRI CASTG 2 cut(s) 500, 518
Van91I CCANNNNNTGG 1 cut(s) 457
VpaK11BI GGWCC 4 cut(s) 490, 937, 1015, 1424
XceI RCATGY 2 cut(s) 760, 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XhoI CTCGAG 1 cut(s) 896
XmaI CCCGGG 1 cut(s) 610
XspI CTAG 3 cut(s) 32, 344, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.