Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014901

Physical Location & Seq

Genomic Coordinates Reverse (-)

131487 .. 133106

Gene Structure 1620 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1002100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGTAGCCGTACGCGTGAAGGAAGCAGAAGCACCATTACTACTGCTAACGGTGTCTGCTCTTCTCCTCCTAATAATTAGCAGCTGCGCCGTGGCCACCACCATCACCAATACTACAACTACTTCCCTTACGTACCCTGAGTTTCAAGAGCTGAACGTGAAGGAAACAATCACGGCGATTCAAAGTAATGACAGGGCACCTAGGGCTGCATACAACGTCACCAGCAGCACCACCACCGGCAGCAAATTAAATGATGATGATGATGAAGATGATATAGATCATGAAGAATATGAAAGAAGCACTAATGGCGGATCCGGAGTAGGAAAATGGAAGGTGAAGGTGGTCCACAGGGATAAGATGTCGTCGCAATATGCAAACAGGAATGGGAATGAAGGCAGCCATACTTCTCATAGCCACCTTTTCCGCGCACGCATGAAAAGGGATGCCAAAAGGTTTGCTAGCCTCACGCGGCGCCTCCTCAACTACTCAGACTCAGAGTTAGAGTCGTTGGATTTTGGGTCAGGGGTGGTGTCGGGTATGGAGCAAGGGAGTGGAGAATACTTCGTGAGGATCGGAGTGGGGAGCCCACCACGAAGCCAATACATGGTGATTGACTCCGGCAGTGACATCGTGTGGGTTCAGTGCCAGCCTTGCACCCATTGTTACCACCAGTCCGACCCCATCTTTGACCCCGCCCGCTCTGCTTCGTATTCCAGCGTCTCCTGTGCCTCCTCCCTCTGCGGCCGCCTCCAGAACCCCGGTTGCCACTCCGGTCGATGCCTCTACGAGGCCTCCTACGGCGACGGATCCTACACCCAGGGCACGCTTGCCCTCGAAACTCTTACCTTGGGACGCGCCCTCCTCCGAAACGTAGCCATCGGCTGCGGGCACATGAATCGCGGCATGTTTGTGGGAGCCGCTGGTCTCTTGGGCCTTGGAGGTGGGGCCATGTCATTTGTTGGTCAGCTCGCCGGCCTGACCGGAGGTGCCTTCACTTATTGTTTGGTCAGCCGTGGCGGCCGCTCTAGTACTACAAGTACCAACGCAGCCGGCGGAGGAGGAGGAGGGTCCCTGGAGTTCGGTCGTGGGGCGATGCCCGTGGGTGCTGCATGGGTCCCGCTGATCCGGAACCCGCGGGCCCCGAGTTTGTATTTTGTGGGGCTGGCGGGCCTTGGAGTTGGAGGGATGCGGGTGCCCATATCCGGAGACGTATTCCGGCAAACCGAGATGGGGTACGGAGGGGTTGTGATGGACACGGGCACCGCCGTGACAAGGTTCCCGACGTTGGCTTACGAGGCCTTCCGCGATACCTTTCTTCAGCAGACGGCCAGCCTCCCTCGGCTGTCGTCGGGAGTGTCCATCTTTGACACATGCTATGACCTGAACGGCTTCGTGTCGGTTCGGGTGCCGACCGTGTCGTTTTATTTTTCGGGTGGGCCCATCCTGACGCTTCCGGCGAGCAATTTTCTGATTCCGGTGGACGAGAAGGGGACCTTTTGCTTTGCATTTGCTCCTTCGGCCTCCGGACTTTCCATAATAGGCAACATCCAGCAAGAGGGCATCCAAATCTCATTTGATGGAGCCAATGGTTTTGTGGGTTTTGGACCCAATGTCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

540

Amino Acids

57.0

Weight (kDa)

6.16

Isoelectric Point (pI)

36.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	186 - 240	3.9e-07	Eukaryotic aspartyl protease
TAXi_N	PF14543	187 - 341	1.1e-46	Xylanase inhibitor N-terminal
Asp	PF00026	264 - 535	3.3e-06	Eukaryotic aspartyl protease
TAXi_C	PF14541	384 - 535	3.8e-28	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20015
fragaria_vesca	FvH4_4g37260
malus_domestica	MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica	Prupe.1G350500_v2.0.a1
pyrus_communis	pycom13g00180 pycom16g00070
rosa_chinensis	RchiOBHm_Chr4g0447081
rosa_laevigata	RLG00000005607
rosa_roxburghii	Rroxscaffold_5G00387390
rosa_rugosa	Rorug04G0377800
rosa_samantha	Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana	Rw4G037560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	6 cut(s)	196, 471, 986, 1192, 1259, 1405
AccB7I	CCANNNNNTGG	1 cut(s)	604
AccBSI	CCGCTC	2 cut(s)	699, 1023
AccII	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
AccIII	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
AclWI	GGATC	6 cut(s)	306, 319, 578, 801, 814, 1117
AcoI	YGGCCR	4 cut(s)	93, 742, 1018, 1326
AcuI	CTGAAG	1 cut(s)	1301
AcyI	GRCGYC	1 cut(s)	472
AfaI	GTAC	5 cut(s)	12, 134, 1030, 1039, 1235
AfiI	CCNNNNNNNGG	7 cut(s)	604, 787, 1202, 1230, 1272, 1285, 1555
AflIII	ACRYGT	1 cut(s)	13
AgsI	TTSAA	2 cut(s)	146, 182
AhdI	GACNNNNNGTC	1 cut(s)	1610
AjnI	CCWGG	2 cut(s)	816, 1071
AleI	CACNNNNGTG	1 cut(s)	1265
AluBI	AGCT	3 cut(s)	84, 151, 967
AluI	AGCT	3 cut(s)	84, 151, 967
Alw26I	GTCTC	3 cut(s)	724, 929, 1200
AlwI	GGATC	6 cut(s)	306, 319, 578, 801, 814, 1117
Ama87I	CYCGRG	1 cut(s)	1141
Aor13HI	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
ApaI	GGGCCC	2 cut(s)	1141, 1440
ApeKI	GCWGC	9 cut(s)	81, 84, 206, 225, 240, 396, 882, 1046, 1106
ArsI	GACNNNNNNTTYG	2 cut(s)	668, 700
Asp700I	GAANNNNTTC	1 cut(s)	1388
AspA2I	CCTAGG	1 cut(s)	200
AspLEI	GCGC	4 cut(s)	89, 428, 474, 857
AsuC2I	CCSGG	1 cut(s)	759
AsuHPI	GGTGA	4 cut(s)	97, 211, 346, 619
AsuNHI	GCTAGC	1 cut(s)	458
AvaI	CYCGRG	1 cut(s)	1141
AvaII	GGWCC	5 cut(s)	343, 1068, 1114, 1491, 1604
AvrII	CCTAGG	1 cut(s)	200
BaeGI	GKGCMC	7 cut(s)	199, 824, 891, 1141, 1197, 1262, 1440
BalI	TGGCCA	1 cut(s)	95
BamHI	GGATCC	2 cut(s)	311, 806
BanI	GGYRCC	6 cut(s)	196, 471, 986, 1192, 1259, 1405
BanII	GRGCYC	3 cut(s)	587, 1141, 1440
BarI	GAAGNNNNNNTAC	2 cut(s)	21, 53
BbvI	GCAGC	9 cut(s)	71, 93, 193, 237, 252, 408, 869, 1058, 1093
BccI	CCATC	8 cut(s)	110, 689, 884, 1222, 1243, 1367, 1448, 1571
BceAI	ACGGC	7 cut(s)	74, 189, 814, 996, 1250, 1341, 1402
BcgI	CGANNNNNNTGC	4 cut(s)	610, 644, 878, 912
BciT130I	CCWGG	2 cut(s)	818, 1073
BcnI	CCSGG	1 cut(s)	759
BcoDI	GTCTC	3 cut(s)	724, 929, 1200
BfaI	CTAG	3 cut(s)	201, 459, 1026
BfoI	RGCGCY	1 cut(s)	475
BglI	GCCNNNNNGGC	1 cut(s)	1017
BlnI	CCTAGG	1 cut(s)	200
BmcAI	AGTACT	1 cut(s)	1030
Bme1390I	CCNGG	3 cut(s)	759, 818, 1073
Bme18I	GGWCC	5 cut(s)	343, 1068, 1114, 1491, 1604
BmeRI	GACNNNNNGTC	1 cut(s)	1610
BmeT110I	CYCGRG	1 cut(s)	1141
BmrFI	CCNGG	3 cut(s)	759, 818, 1073
BmsI	GCATC	5 cut(s)	433, 767, 1083, 1176, 1569
BmtI	GCTAGC	1 cut(s)	462
BpmI	CTGGAG	2 cut(s)	734, 1094
BpuMI	CCSGG	1 cut(s)	759
BsaAI	YACGTR	1 cut(s)	132
BsaBI	GATNNNNATC	1 cut(s)	276
BsaHI	GRCGYC	1 cut(s)	472
BsaI	GGTCTC	1 cut(s)	929
BsaWI	WCCGGW	7 cut(s)	314, 770, 980, 1125, 1202, 1474, 1523
BsaXI	ACNNNNNCTCC	6 cut(s)	776, 806, 843, 873, 1230, 1260
Bsc4I	CCNNNNNNNGG	7 cut(s)	604, 787, 1202, 1230, 1272, 1285, 1555
Bse118I	RCCGGY	3 cut(s)	236, 971, 1049
Bse1I	ACTGG	1 cut(s)	670
Bse8I	GATNNNNATC	1 cut(s)	276
BseAI	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
BseBI	CCWGG	2 cut(s)	818, 1073
BseGI	GGATG	5 cut(s)	448, 1191, 1440, 1545, 1560
BseJI	GATNNNNATC	1 cut(s)	276
BseLI	CCNNNNNNNGG	7 cut(s)	604, 787, 1202, 1230, 1272, 1285, 1555
BseMII	CTCAG	3 cut(s)	129, 501, 507
BseNI	ACTGG	1 cut(s)	670
BseRI	GAGGAG	7 cut(s)	56, 467, 721, 851, 1071, 1074, 1077
BseSI	GKGCMC	7 cut(s)	199, 824, 891, 1141, 1197, 1262, 1440
BseX3I	CGGCCG	2 cut(s)	742, 1018
BseXI	GCAGC	9 cut(s)	71, 93, 193, 237, 252, 408, 869, 1058, 1093
Bsh1236I	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
Bsh1285I	CGRYCG	5 cut(s)	745, 775, 1021, 1084, 1413
BshNI	GGYRCC	6 cut(s)	196, 471, 986, 1192, 1259, 1405
BsiEI	CGRYCG	5 cut(s)	745, 775, 1021, 1084, 1413
BsiHKCI	CYCGRG	1 cut(s)	1141
BsiWI	CGTACG	1 cut(s)	10
BslFI	GGGAC	4 cut(s)	864, 1054, 1100, 1504
BslI	CCNNNNNNNGG	7 cut(s)	604, 787, 1202, 1230, 1272, 1285, 1555
BsmAI	GTCTC	3 cut(s)	724, 929, 1200
BsmBI	CGTCTC	2 cut(s)	724, 1200
BsmFI	GGGAC	4 cut(s)	864, 1054, 1100, 1504
Bso31I	GGTCTC	1 cut(s)	929
BsoBI	CYCGRG	1 cut(s)	1141
Bsp120I	GGGCCC	2 cut(s)	1137, 1436
Bsp1286I	GDGCHC	8 cut(s)	199, 587, 824, 891, 1141, 1197, 1262, 1440
Bsp13I	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
Bsp143I	GATC	5 cut(s)	277, 311, 570, 806, 1122
BspCNI	CTCAG	3 cut(s)	130, 500, 506
BspEI	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
BspFNI	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
BspHI	TCATGA	1 cut(s)	280
BspOI	GCTAGC	1 cut(s)	462
BspPI	GGATC	6 cut(s)	306, 319, 578, 801, 814, 1117
BspQI	GCTCTTC	1 cut(s)	66
BspT107I	GGYRCC	6 cut(s)	196, 471, 986, 1192, 1259, 1405
BspTNI	GGTCTC	1 cut(s)	929
BsrBI	CCGCTC	2 cut(s)	699, 1023
BsrFI	RCCGGY	3 cut(s)	236, 971, 1049
BsrI	ACTGG	1 cut(s)	670
BssAI	RCCGGY	3 cut(s)	236, 971, 1049
BssMI	GATC	5 cut(s)	277, 311, 570, 806, 1122
BssNI	GRCGYC	1 cut(s)	472
BssT1I	CCWWGG	4 cut(s)	200, 846, 934, 1171
Bst2UI	CCWGG	2 cut(s)	818, 1073
Bst4CI	ACNGT	2 cut(s)	52, 1414
Bst6I	CTCTTC	1 cut(s)	66
BstACI	GRCGYC	1 cut(s)	472
BstBAI	YACGTR	1 cut(s)	132
BstDEI	CTNAG	3 cut(s)	138, 487, 493
BstDSI	CCRYGG	4 cut(s)	90, 1012, 1098, 1133
BstENI	CCTNNNNNAGG	1 cut(s)	785
BstF5I	GGATG	5 cut(s)	448, 1191, 1440, 1545, 1560
BstFNI	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
BstH2I	RGCGCY	1 cut(s)	475
BstHHI	GCGC	4 cut(s)	89, 428, 474, 857
BstKTI	GATC	5 cut(s)	280, 314, 573, 809, 1125
BstMAI	GTCTC	3 cut(s)	724, 929, 1200
BstMBI	GATC	5 cut(s)	277, 311, 570, 806, 1122
BstMCI	CGRYCG	5 cut(s)	745, 775, 1021, 1084, 1413
BstMWI	GCNNNNNNNGC	7 cut(s)	203, 306, 651, 701, 1017, 1295, 1517
BstNI	CCWGG	2 cut(s)	818, 1073
BstNSI	RCATGY	2 cut(s)	907, 1374
BstSCI	CCNGG	3 cut(s)	757, 816, 1071
BstSLI	GKGCMC	7 cut(s)	199, 824, 891, 1141, 1197, 1262, 1440
BstSNI	TACGTA	1 cut(s)	132
BstUI	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
BstV1I	GCAGC	9 cut(s)	71, 93, 193, 237, 252, 408, 869, 1058, 1093
BstX2I	RGATCY	2 cut(s)	311, 806
BstYI	RGATCY	2 cut(s)	311, 806
BstZI	CGGCCG	2 cut(s)	742, 1018
BtgI	CCRYGG	4 cut(s)	90, 1012, 1098, 1133
BtgZI	GCGATG	1 cut(s)	1106
BtsCI	GGATG	5 cut(s)	448, 1191, 1440, 1545, 1560
BtsI	GCAGTG	1 cut(s)	628
BtsIMutI	CAGTG	2 cut(s)	628, 647
CciI	TCATGA	1 cut(s)	280
CciNI	GCGGCCGC	2 cut(s)	742, 1018
CfoI	GCGC	4 cut(s)	89, 428, 474, 857
Cfr10I	RCCGGY	3 cut(s)	236, 971, 1049
Cfr42I	CCGCGG	1 cut(s)	1136
CseI	GACGC	3 cut(s)	706, 861, 1456
Csp6I	GTAC	5 cut(s)	11, 133, 1029, 1038, 1234
CviAII	CATG	8 cut(s)	281, 433, 604, 892, 904, 949, 1110, 1371
CviQI	GTAC	5 cut(s)	11, 133, 1029, 1038, 1234
DdeI	CTNAG	3 cut(s)	138, 487, 493
DinI	GGCGCC	1 cut(s)	473
DpnI	GATC	5 cut(s)	279, 313, 572, 808, 1124
DpnII	GATC	5 cut(s)	277, 311, 570, 806, 1122
DriI	GACNNNNNGTC	1 cut(s)	1610
EaeI	YGGCCR	4 cut(s)	93, 742, 1018, 1326
EagI	CGGCCG	2 cut(s)	742, 1018
Eam1104I	CTCTTC	1 cut(s)	66
Eam1105I	GACNNNNNGTC	1 cut(s)	1610
EarI	CTCTTC	1 cut(s)	66
EciI	GGCGGA	2 cut(s)	324, 1068
EclXI	CGGCCG	2 cut(s)	742, 1018
Eco105I	TACGTA	1 cut(s)	132
Eco130I	CCWWGG	4 cut(s)	200, 846, 934, 1171
Eco147I	AGGCCT	2 cut(s)	791, 1298
Eco24I	GRGCYC	3 cut(s)	587, 1141, 1440
Eco31I	GGTCTC	1 cut(s)	929
Eco47I	GGWCC	5 cut(s)	343, 1068, 1114, 1491, 1604
Eco52I	CGGCCG	2 cut(s)	742, 1018
Eco57I	CTGAAG	1 cut(s)	1301
Eco88I	CYCGRG	1 cut(s)	1141
EcoNI	CCTNNNNNAGG	1 cut(s)	785
EcoO109I	RGGNCCY	4 cut(s)	1068, 1114, 1138, 1491
EcoRII	CCWGG	2 cut(s)	816, 1071
EcoT14I	CCWWGG	4 cut(s)	200, 846, 934, 1171
EcoT38I	GRGCYC	3 cut(s)	587, 1141, 1440
EgeI	GGCGCC	1 cut(s)	473
EheI	GGCGCC	1 cut(s)	473
ErhI	CCWWGG	4 cut(s)	200, 846, 934, 1171
Esp3I	CGTCTC	2 cut(s)	724, 1200
FaeI	CATG	8 cut(s)	284, 436, 607, 895, 907, 952, 1113, 1374
FalI	AAGNNNNNCTT	2 cut(s)	1478, 1510
FaqI	GGGAC	4 cut(s)	864, 1054, 1100, 1504
FatI	CATG	8 cut(s)	280, 432, 603, 891, 903, 948, 1109, 1370
FauI	CCCGC	8 cut(s)	700, 704, 878, 1125, 1128, 1140, 1159, 1182
FokI	GGATG	5 cut(s)	455, 1198, 1427, 1532, 1547
FriOI	GRGCYC	3 cut(s)	587, 1141, 1440
FspBI	CTAG	3 cut(s)	201, 459, 1026
GlaI	GCGC	4 cut(s)	88, 427, 473, 856
GsuI	CTGGAG	2 cut(s)	734, 1094
HaeII	RGCGCY	1 cut(s)	475
HgaI	GACGC	3 cut(s)	706, 861, 1456
HhaI	GCGC	4 cut(s)	89, 428, 474, 857
Hin1I	GRCGYC	1 cut(s)	472
Hin1II	CATG	8 cut(s)	284, 436, 607, 895, 907, 952, 1113, 1374
Hin6I	GCGC	4 cut(s)	87, 426, 472, 855
HinP1I	GCGC	4 cut(s)	87, 426, 472, 855
HinfI	GANTC	6 cut(s)	178, 491, 503, 614, 895, 1471
HphI	GGTGA	4 cut(s)	97, 211, 346, 619
Hpy166II	GTNNAC	2 cut(s)	346, 1480
Hpy188I	TCNGA	6 cut(s)	490, 496, 575, 676, 866, 1470
Hpy8I	GTNNAC	2 cut(s)	346, 1480
Hpy99I	CGWCG	4 cut(s)	367, 806, 1285, 1351
HpyAV	CCTTC	9 cut(s)	13, 154, 325, 331, 386, 1000, 1309, 1480, 1524
HpyCH4III	ACNGT	2 cut(s)	52, 1414
HpyCH4IV	ACGT	6 cut(s)	131, 156, 216, 870, 1209, 1283
HpyCH4V	TGCA	5 cut(s)	209, 374, 654, 1109, 1505
HpyF10VI	GCNNNNNNNGC	7 cut(s)	203, 306, 651, 701, 1017, 1295, 1517
HpyF3I	CTNAG	3 cut(s)	138, 487, 493
HpySE526I	ACGT	6 cut(s)	131, 156, 216, 870, 1209, 1283
Hsp92I	GRCGYC	1 cut(s)	472
Hsp92II	CATG	8 cut(s)	284, 436, 607, 895, 907, 952, 1113, 1374
HspAI	GCGC	4 cut(s)	87, 426, 472, 855
KasI	GGCGCC	1 cut(s)	471
KflI	GGGWCCC	2 cut(s)	1068, 1114
Kpn2I	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
KroI	GCCGGC	2 cut(s)	971, 1049
KroNI	GCCGGC	2 cut(s)	973, 1051
KspI	CCGCGG	1 cut(s)	1136
Kzo9I	GATC	5 cut(s)	277, 311, 570, 806, 1122
LguI	GCTCTTC	1 cut(s)	66
LmnI	GCTCC	5 cut(s)	541, 582, 914, 1516, 1580
Lsp1109I	GCAGC	9 cut(s)	71, 93, 193, 237, 252, 408, 869, 1058, 1093
LweI	GCATC	5 cut(s)	433, 767, 1083, 1176, 1569
MaeI	CTAG	3 cut(s)	201, 459, 1026
MaeII	ACGT	6 cut(s)	131, 156, 216, 870, 1209, 1283
MaeIII	GTNAC	4 cut(s)	217, 623, 662, 1267
MalI	GATC	5 cut(s)	279, 313, 572, 808, 1124
MbiI	CCGCTC	2 cut(s)	699, 1023
MboI	GATC	5 cut(s)	277, 311, 570, 806, 1122
MboII	GAAGA	4 cut(s)	53, 278, 296, 1307
MflI	RGATCY	2 cut(s)	311, 806
MhlI	GDGCHC	8 cut(s)	199, 587, 824, 891, 1141, 1197, 1262, 1440
MlsI	TGGCCA	1 cut(s)	95
MluCI	AATT	3 cut(s)	75, 245, 1462
MluI	ACGCGT	1 cut(s)	13
MluNI	TGGCCA	1 cut(s)	95
Mly113I	GGCGCC	1 cut(s)	472
MlyI	GAGTC	3 cut(s)	485, 512, 608
MmeI	TCCRAC	3 cut(s)	489, 699, 1159
Mox20I	TGGCCA	1 cut(s)	95
MroI	TCCGGA	4 cut(s)	314, 1125, 1202, 1523
MroNI	GCCGGC	2 cut(s)	971, 1049
MroXI	GAANNNNTTC	1 cut(s)	1388
MscI	TGGCCA	1 cut(s)	95
MseI	TTAA	1 cut(s)	248
MslI	CAYNNNNRTG	2 cut(s)	908, 1265
Msp20I	TGGCCA	1 cut(s)	95
MspA1I	CMGCKG	4 cut(s)	84, 920, 1120, 1135
MspR9I	CCNGG	3 cut(s)	759, 818, 1073
MvaI	CCWGG	2 cut(s)	818, 1073
MvnI	CGCG	7 cut(s)	15, 426, 469, 855, 900, 1135, 1305
MwoI	GCNNNNNNNGC	7 cut(s)	203, 306, 651, 701, 1017, 1295, 1517
NaeI	GCCGGC	2 cut(s)	973, 1051
NarI	GGCGCC	1 cut(s)	472
NciI	CCSGG	1 cut(s)	759
NdeII	GATC	5 cut(s)	277, 311, 570, 806, 1122
NgoMIV	GCCGGC	2 cut(s)	971, 1049
NheI	GCTAGC	1 cut(s)	458
NlaIII	CATG	8 cut(s)	284, 436, 607, 895, 907, 952, 1113, 1374
NmeAIII	GCCGAG	1 cut(s)	1318
NmuCI	GTSAC	3 cut(s)	217, 623, 1267
NotI	GCGGCCGC	2 cut(s)	742, 1018
NspI	RCATGY	2 cut(s)	907, 1374
OliI	CACNNNNGTG	1 cut(s)	1265
PagI	TCATGA	1 cut(s)	280
PasI	CCCWGGG	1 cut(s)	817
PceI	AGGCCT	2 cut(s)	791, 1298
PciSI	GCTCTTC	1 cut(s)	66
PcsI	WCGNNNNNNNCGW	1 cut(s)	1454
PdiI	GCCGGC	2 cut(s)	973, 1051
PdmI	GAANNNNTTC	1 cut(s)	1388
PfeI	GAWTC	3 cut(s)	178, 895, 1471
Pfl23II	CGTACG	1 cut(s)	10
PflFI	GACNNNGTC	1 cut(s)	1414
PflMI	CCANNNNNTGG	1 cut(s)	604
PleI	GAGTC	3 cut(s)	485, 511, 608
PluTI	GGCGCC	1 cut(s)	475
PpsI	GAGTC	3 cut(s)	485, 511, 608
Ppu21I	YACGTR	1 cut(s)	132
PpuMI	RGGWCCY	3 cut(s)	1068, 1114, 1491
Psp5II	RGGWCCY	3 cut(s)	1068, 1114, 1491
Psp6I	CCWGG	2 cut(s)	816, 1071
PspGI	CCWGG	2 cut(s)	816, 1071
PspLI	CGTACG	1 cut(s)	10
PspOMI	GGGCCC	2 cut(s)	1137, 1436
PspPPI	RGGWCCY	3 cut(s)	1068, 1114, 1491
PsuI	RGATCY	2 cut(s)	311, 806
PsyI	GACNNNGTC	1 cut(s)	1414
PvuII	CAGCTG	1 cut(s)	84
RsaI	GTAC	5 cut(s)	12, 134, 1030, 1039, 1235
RsaNI	GTAC	5 cut(s)	11, 133, 1029, 1038, 1234
RseI	CAYNNNNRTG	2 cut(s)	908, 1265
SacII	CCGCGG	1 cut(s)	1136
SapI	GCTCTTC	1 cut(s)	66
SaqAI	TTAA	1 cut(s)	248
Sau3AI	GATC	5 cut(s)	277, 311, 570, 806, 1122
ScaI	AGTACT	1 cut(s)	1030
SchI	GAGTC	3 cut(s)	485, 512, 608
ScrFI	CCNGG	3 cut(s)	759, 818, 1073
SduI	GDGCHC	8 cut(s)	199, 587, 824, 891, 1141, 1197, 1262, 1440
SfaNI	GCATC	5 cut(s)	433, 767, 1083, 1176, 1569
SfoI	GGCGCC	1 cut(s)	473
Sfr303I	CCGCGG	1 cut(s)	1136
SgrBI	CCGCGG	1 cut(s)	1136
SinI	GGWCC	5 cut(s)	343, 1068, 1114, 1491, 1604
SmiMI	CAYNNNNRTG	2 cut(s)	908, 1265
SnaBI	TACGTA	1 cut(s)	132
Sse9I	AATT	3 cut(s)	75, 245, 1462
SseBI	AGGCCT	2 cut(s)	791, 1298
SspDI	GGCGCC	1 cut(s)	471
SspMI	CTAG	3 cut(s)	201, 459, 1026
StuI	AGGCCT	2 cut(s)	791, 1298
StyD4I	CCNGG	3 cut(s)	757, 816, 1071
StyI	CCWWGG	4 cut(s)	200, 846, 934, 1171
TaaI	ACNGT	2 cut(s)	52, 1414
TaiI	ACGT	6 cut(s)	134, 159, 219, 873, 1212, 1286
TaqI	TCGA	2 cut(s)	775, 834
TaqII	GACCGA	1 cut(s)	1070
TasI	AATT	3 cut(s)	75, 245, 1462
TatI	WGTACW	1 cut(s)	1028
TauI	GCSGC	7 cut(s)	472, 744, 747, 903, 920, 1020, 1023
TfiI	GAWTC	3 cut(s)	178, 895, 1471
Tru1I	TTAA	1 cut(s)	248
Tru9I	TTAA	1 cut(s)	248
TscAI	CASTG	2 cut(s)	628, 647
TseFI	GTSAC	3 cut(s)	217, 623, 1267
TseI	GCWGC	9 cut(s)	81, 84, 206, 225, 240, 396, 882, 1046, 1106
Tsp45I	GTSAC	3 cut(s)	217, 623, 1267
TspDTI	ATGAA	6 cut(s)	279, 297, 306, 405, 449, 908
TspGWI	ACGGA	2 cut(s)	819, 1251
TspRI	CASTG	2 cut(s)	628, 647
Tth111I	GACNNNGTC	1 cut(s)	1414
Van91I	CCANNNNNTGG	1 cut(s)	604
VpaK11BI	GGWCC	5 cut(s)	343, 1068, 1114, 1491, 1604
XagI	CCTNNNNNAGG	1 cut(s)	785
XceI	RCATGY	2 cut(s)	907, 1374
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1591
XmaJI	CCTAGG	1 cut(s)	200
XmnI	GAANNNNTTC	1 cut(s)	1388
XspI	CTAG	3 cut(s)	201, 459, 1026
ZrmI	AGTACT	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.