Rh4AG439100

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014901
Physical Location & Seq
Forward (+)
73077645 .. 73079634
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG439100.1

Sequence Viewer

Length: 1464 bp
ATGGAGGGGATACTATTACTCTGCGTGGCTCTAGTCATAATTACAGGTTTCGGTATTAGCGGCACCGCCACCACCATCACCACTTACCCCGATTTCCAAGAGTTGTACGTCAAACAAACAATCGCTTCCGCTTCTGCTGCCACCAAGCTATACAAGGAAGAAGAAGAAGATGATGATGATGGTGGTAGCGAAAGCGGAGGAGCAAGATGGAAGGTGAAGGTGGTTCATAGAGATAAGATATTCCCGCAAGCACCATACAACAAGAGCAGCGGCAGCAATTCACATCATCATCATCTGTTTCATGCACGCATCAACAGAGACGTGAAAAGGGTTGATAGCCTTATGCGTCGCCTCGGAAATAGTAAACTAGTCGTCAATTCCGCCACGGACTTTGGAACGGAGGTGGTTTCGGGGATGGCGCAAGGCAGCGGTGAATACTTTGTTAGGATCGGCGTTGGCAGCCCTCCCAGAAGCCAATACGTGGTCATTGACTCCGGCAGCGATATTGTGTGGGTCCAGTGTCAACCTTGTAGCCAGTGTTATCAGCAGTCCGACCCGATCTTCGACCCGGCACACTCTGCTTCTTACTCCGGCGTGTCTTGCACCTCCACCGTGTGTGGCCGCCTCCAGAACCCGGGCTGCCACGCAGGTCGGTGCCGGTACGAGGCTTCCTACGGCGACGGCTCCTACACGCAGGGCACTCTCGCCCTCGAAACAGTCACCTTCGGCCGCGCTCTCATCCGCAACGTGGCCATCGGCTGCGGCCACATGAACCGGGGCATGTTCGTCGGAGCCGCAGGTCTTTTGGGCCTCGGGAGTGGGCCCATGTCATTCGTCGGCCAGCTGGGCGGCCAGACTGGCGGTGCATTTTCCTACTGCTTGGTCAGCCGGGGTGACGGTGACTCCACCACCGGCTCACTCGAGTTCGGGCGTGCCGCGATGCCCGTGGGAGCTGCGTGGGTCCCTCTGATCCGGAACCAACGGGCCCCGAGTTTCTACTACGTTGGCCTCGCGGGCCTTGGTGTCGGAGGCATGCGGGTCCCAATATCCGAAGACGTGTTCCGGCAGACGGAGCTCGGTGACGGAGGCGTTGTTATGGACACCGGAACGGCCGTCACCAGGTTCCCGACGTTGGCTTATCAGGCATTCCGCGACGCGTTTCTTCAGCAAACTGCAAACCTCCCTCGGGTCTCCGGAGTCTCCATCTTTGACACGTGTTTTGACCTGAACGGCTTTGTGTCGGTTCGGGTACCGACCGTTTCCTTTTATTTCTCGGGCGGGCCCATCCTGACCCTTCCGGCAAGGAACTTTCTGATTCCGGTGGATGACAAGGGTACGTTTTGTTTGGCATTTGCTCCTTCCGCTGCGGGGCTTTCCATAATAGGGAATATTCAGCAAGAGGGCATTCAAATCTCATTTGATGGAGCTAATGGTTTTGTAGGATTTGGACCCAATGTCTGCTAG

Protein Analysis

487

Amino Acids

51.63

Weight (kDa)

6.15

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 145 - 197 1e-07 Eukaryotic aspartyl protease
TAXi_N PF14543 146 - 310 8.2e-48 Xylanase inhibitor N-terminal
Asp PF00026 222 - 483 6e-08 Eukaryotic aspartyl protease
TAXi_C PF14541 333 - 483 5.1e-31 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014901)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20015
fragaria_vesca FvH4_4g37260
malus_domestica MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica Prupe.1G350500_v2.0.a1
pyrus_communis pycom13g00180 pycom16g00070
rosa_chinensis RchiOBHm_Chr4g0447081
rosa_laevigata RLG00000005607
rosa_roxburghii Rroxscaffold_5G00387390
rosa_rugosa Rorug04G0377800
rosa_samantha Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana Rw4G037560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 638, 788
Acc65I GGTACC 1 cut(s) 1249
AccB1I GGYRCC 3 cut(s) 62, 654, 1249
AccB7I CCANNNNNTGG 1 cut(s) 481
AccII CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
AccIII TCCGGA 2 cut(s) 972, 1193
AclWI GGATC 2 cut(s) 455, 964
AcoI YGGCCR 7 cut(s) 619, 727, 750, 763, 838, 850, 1110
AcuI CTGAAG 1 cut(s) 1148
AcvI CACGTG 1 cut(s) 1215
AdeI CACNNNGTG 1 cut(s) 615
AfaI GTAC 4 cut(s) 107, 662, 1251, 1336
AflIII ACRYGT 4 cut(s) 1056, 1155, 1212, 1214
AgsI TTSAA 1 cut(s) 1409
AhdI GACNNNNNGTC 1 cut(s) 1454
AhlI ACTAGT 1 cut(s) 367
AjiI CACGTC 2 cut(s) 322, 1057
AjnI CCWGG 1 cut(s) 1118
AluBI AGCT 5 cut(s) 148, 844, 953, 1075, 1427
AluI AGCT 5 cut(s) 148, 844, 953, 1075, 1427
Alw21I GWGCWC 1 cut(s) 1077
Alw26I GTCTC 3 cut(s) 312, 1195, 1204
AlwI GGATC 2 cut(s) 455, 964
Ama87I CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
Aor13HI TCCGGA 2 cut(s) 972, 1193
ApaI GGGCCC 3 cut(s) 825, 988, 1284
ArsI GACNNNNNNTTYG 4 cut(s) 545, 577, 1202, 1234
Asp718I GGTACC 1 cut(s) 1249
AspLEI GCGC 2 cut(s) 421, 734
AsuC2I CCSGG 5 cut(s) 569, 635, 636, 776, 890
AsuHPI GGTGA 8 cut(s) 70, 226, 443, 712, 905, 911, 1091, 1108
AvaI CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
AvaII GGWCC 4 cut(s) 514, 961, 1039, 1448
BaeGI GKGCMC 4 cut(s) 701, 825, 988, 1284
BalI TGGCCA 1 cut(s) 752
BanI GGYRCC 3 cut(s) 62, 654, 1249
BanII GRGCYC 4 cut(s) 825, 988, 1077, 1284
BbrPI CACGTG 1 cut(s) 1215
BbsI GAAGAC 1 cut(s) 1059
Bbv12I GWGCWC 1 cut(s) 1077
BccI CCATC 8 cut(s) 83, 173, 201, 409, 761, 1211, 1292, 1415
BceAI ACGGC 5 cut(s) 691, 697, 1097, 1125, 1246
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 1 cut(s) 1120
BciVI GTATCC 1 cut(s) 3
BcnI CCSGG 5 cut(s) 569, 635, 636, 776, 890
BcoDI GTCTC 3 cut(s) 312, 1195, 1204
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 3 cut(s) 32, 368, 1462
BfuAI ACCTGC 2 cut(s) 638, 788
BfuI GTATCC 1 cut(s) 3
BglI GCCNNNNNGGC 3 cut(s) 846, 858, 1014
Bme1390I CCNGG 6 cut(s) 569, 635, 636, 776, 890, 1120
Bme18I GGWCC 4 cut(s) 514, 961, 1039, 1448
BmeRI GACNNNNNGTC 1 cut(s) 1454
BmeT110I CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
BmgBI CACGTC 2 cut(s) 322, 1057
BmrFI CCNGG 6 cut(s) 569, 635, 636, 776, 890, 1120
BmsI GCATC 2 cut(s) 318, 930
BpiI GAAGAC 1 cut(s) 1059
BpmI CTGGAG 1 cut(s) 611
BpuMI CCSGG 5 cut(s) 569, 635, 636, 776, 890
BsaAI YACGTR 2 cut(s) 481, 1215
BsaI GGTCTC 1 cut(s) 1195
BsaJI CCNNGG 9 cut(s) 352, 384, 634, 775, 811, 889, 945, 1018, 1184
BsaWI WCCGGW 4 cut(s) 972, 1103, 1193, 1318
BsaXI ACNNNNNCTCC 4 cut(s) 887, 917, 1077, 1107
Bse118I RCCGGY 2 cut(s) 657, 911
Bse1I ACTGG 3 cut(s) 517, 535, 862
BseAI TCCGGA 2 cut(s) 972, 1193
BseBI CCWGG 1 cut(s) 1120
BseDI CCNNGG 9 cut(s) 352, 384, 634, 775, 811, 889, 945, 1018, 1184
BseGI GGATG 4 cut(s) 420, 738, 1284, 1330
BseNI ACTGG 3 cut(s) 517, 535, 862
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 4 cut(s) 701, 825, 988, 1284
BseX3I CGGCCG 2 cut(s) 727, 1110
BseYI CCCAGC 1 cut(s) 844
Bsh1236I CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
Bsh1285I CGRYCG 3 cut(s) 730, 1113, 1257
BshNI GGYRCC 3 cut(s) 62, 654, 1249
BsiEI CGRYCG 3 cut(s) 730, 1113, 1257
BsiHKAI GWGCWC 1 cut(s) 1077
BsiHKCI CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
BslFI GGGAC 2 cut(s) 947, 1025
BsmAI GTCTC 3 cut(s) 312, 1195, 1204
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 2 cut(s) 947, 1025
BsmI GAATGC 2 cut(s) 1145, 1404
Bso31I GGTCTC 1 cut(s) 1195
BsoBI CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
Bsp120I GGGCCC 3 cut(s) 821, 984, 1280
Bsp1286I GDGCHC 5 cut(s) 701, 825, 988, 1077, 1284
Bsp13I TCCGGA 2 cut(s) 972, 1193
Bsp143I GATC 3 cut(s) 447, 558, 969
BspEI TCCGGA 2 cut(s) 972, 1193
BspFNI CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
BspMI ACCTGC 2 cut(s) 638, 788
BspPI GGATC 2 cut(s) 455, 964
BspT107I GGYRCC 3 cut(s) 62, 654, 1249
BspTNI GGTCTC 1 cut(s) 1195
BsrFI RCCGGY 2 cut(s) 657, 911
BsrI ACTGG 3 cut(s) 517, 535, 862
BssAI RCCGGY 2 cut(s) 657, 911
BssECI CCNNGG 9 cut(s) 352, 384, 634, 775, 811, 889, 945, 1018, 1184
BssMI GATC 3 cut(s) 447, 558, 969
BssT1I CCWWGG 1 cut(s) 1018
Bst2UI CCWGG 1 cut(s) 1120
Bst4CI ACNGT 4 cut(s) 613, 718, 899, 1258
BstAPI GCANNNNNTGC 1 cut(s) 578
BstBAI YACGTR 2 cut(s) 481, 1215
BstC8I GCNNGC 7 cut(s) 249, 307, 842, 933, 1015, 1034, 1280
BstDSI CCRYGG 2 cut(s) 384, 945
BstF5I GGATG 4 cut(s) 420, 738, 1284, 1330
BstFNI CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
BstHHI GCGC 2 cut(s) 421, 734
BstKTI GATC 3 cut(s) 450, 561, 972
BstMAI GTCTC 3 cut(s) 312, 1195, 1204
BstMBI GATC 3 cut(s) 447, 558, 969
BstMCI CGRYCG 3 cut(s) 730, 1113, 1257
BstNI CCWGG 1 cut(s) 1120
BstNSI RCATGY 2 cut(s) 784, 1036
BstSCI CCNGG 6 cut(s) 567, 633, 634, 774, 888, 1118
BstSLI GKGCMC 4 cut(s) 701, 825, 988, 1284
BstUI CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
BstV2I GAAGAC 1 cut(s) 1059
BstZI CGGCCG 2 cut(s) 727, 1110
BsuI GTATCC 1 cut(s) 3
BtgI CCRYGG 2 cut(s) 384, 945
BtgZI GCGATG 1 cut(s) 953
BtrI CACGTC 2 cut(s) 322, 1057
BtsCI GGATG 4 cut(s) 420, 738, 1284, 1330
BtsIMutI CAGTG 2 cut(s) 524, 542
BveI ACCTGC 2 cut(s) 638, 788
Cac8I GCNNGC 7 cut(s) 249, 307, 842, 933, 1015, 1034, 1280
CfoI GCGC 2 cut(s) 421, 734
Cfr10I RCCGGY 2 cut(s) 657, 911
Cfr9I CCCGGG 1 cut(s) 634
CseI GACGC 2 cut(s) 335, 1163
CsiI ACCWGGT 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 106, 661, 1250, 1335
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 5 cut(s) 302, 769, 781, 826, 1033
CviQI GTAC 4 cut(s) 106, 661, 1250, 1335
DpnI GATC 3 cut(s) 449, 560, 971
DpnII GATC 3 cut(s) 447, 558, 969
DraIII CACNNNGTG 1 cut(s) 615
DriI GACNNNNNGTC 1 cut(s) 1454
EaeI YGGCCR 7 cut(s) 619, 727, 750, 763, 838, 850, 1110
EagI CGGCCG 2 cut(s) 727, 1110
Eam1105I GACNNNNNGTC 1 cut(s) 1454
EciI GGCGGA 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 1075
EclXI CGGCCG 2 cut(s) 727, 1110
Eco130I CCWWGG 1 cut(s) 1018
Eco24I GRGCYC 4 cut(s) 825, 988, 1077, 1284
Eco31I GGTCTC 1 cut(s) 1195
Eco47I GGWCC 4 cut(s) 514, 961, 1039, 1448
Eco52I CGGCCG 2 cut(s) 727, 1110
Eco53kI GAGCTC 1 cut(s) 1075
Eco57I CTGAAG 1 cut(s) 1148
Eco72I CACGTG 1 cut(s) 1215
Eco88I CYCGRG 6 cut(s) 634, 812, 920, 988, 1185, 1273
EcoICRI GAGCTC 1 cut(s) 1075
EcoO109I RGGNCCY 3 cut(s) 961, 985, 1039
EcoRII CCWGG 1 cut(s) 1118
EcoT14I CCWWGG 1 cut(s) 1018
EcoT38I GRGCYC 4 cut(s) 825, 988, 1077, 1284
ErhI CCWWGG 1 cut(s) 1018
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 5 cut(s) 305, 772, 784, 829, 1036
FaqI GGGAC 2 cut(s) 947, 1025
FatI CATG 5 cut(s) 301, 768, 780, 825, 1032
FauI CCCGC 5 cut(s) 252, 1006, 1029, 1271, 1360
FokI GGATG 4 cut(s) 427, 725, 1271, 1337
FriOI GRGCYC 4 cut(s) 825, 988, 1077, 1284
FspBI CTAG 3 cut(s) 32, 368, 1462
GlaI GCGC 2 cut(s) 420, 733
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 1 cut(s) 611
HgaI GACGC 2 cut(s) 335, 1163
HhaI GCGC 2 cut(s) 421, 734
Hin1II CATG 5 cut(s) 305, 772, 784, 829, 1036
Hin6I GCGC 2 cut(s) 419, 732
HinP1I GCGC 2 cut(s) 419, 732
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HinfI GANTC 4 cut(s) 491, 902, 1197, 1315
HphI GGTGA 8 cut(s) 70, 226, 443, 712, 905, 911, 1091, 1108
Hpy166II GTNNAC 2 cut(s) 365, 524
Hpy188I TCNGA 7 cut(s) 356, 553, 791, 969, 1028, 1051, 1314
Hpy188III TCNNGA 6 cut(s) 628, 814, 973, 1126, 1194, 1288
Hpy8I GTNNAC 2 cut(s) 365, 524
Hpy99I CGWCG 6 cut(s) 351, 683, 791, 839, 1132, 1157
HpyAV CCTTC 5 cut(s) 205, 211, 733, 1304, 1368
HpyCH4III ACNGT 4 cut(s) 613, 718, 899, 1258
HpyCH4IV ACGT 9 cut(s) 108, 321, 480, 747, 1002, 1056, 1130, 1214, 1337
HpyCH4V TGCA 4 cut(s) 305, 603, 866, 1175
HpySE526I ACGT 9 cut(s) 108, 321, 480, 747, 1002, 1056, 1130, 1214, 1337
Hsp92II CATG 5 cut(s) 305, 772, 784, 829, 1036
HspAI GCGC 2 cut(s) 419, 732
KflI GGGWCCC 2 cut(s) 961, 1039
Kpn2I TCCGGA 2 cut(s) 972, 1193
KpnI GGTACC 1 cut(s) 1253
Kzo9I GATC 3 cut(s) 447, 558, 969
LmnI GCTCC 7 cut(s) 200, 689, 791, 950, 1072, 1360, 1424
LweI GCATC 2 cut(s) 318, 930
MabI ACCWGGT 1 cut(s) 1118
MaeI CTAG 3 cut(s) 32, 368, 1462
MaeII ACGT 9 cut(s) 108, 321, 480, 747, 1002, 1056, 1130, 1214, 1337
MaeIII GTNAC 5 cut(s) 718, 893, 899, 1079, 1114
MalI GATC 3 cut(s) 449, 560, 971
MboI GATC 3 cut(s) 447, 558, 969
MboII GAAGA 7 cut(s) 170, 173, 176, 179, 553, 1064, 1154
MhlI GDGCHC 5 cut(s) 701, 825, 988, 1077, 1284
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 3 cut(s) 39, 277, 376
MluI ACGCGT 1 cut(s) 1155
MluNI TGGCCA 1 cut(s) 752
MlyI GAGTC 3 cut(s) 485, 896, 1206
MmeI TCCRAC 3 cut(s) 576, 769, 1006
Mox20I TGGCCA 1 cut(s) 752
MroI TCCGGA 2 cut(s) 972, 1193
MscI TGGCCA 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 752
MspA1I CMGCKG 4 cut(s) 270, 429, 844, 1364
MspR9I CCNGG 6 cut(s) 569, 635, 636, 776, 890, 1120
Mva1269I GAATGC 2 cut(s) 1145, 1404
MvaI CCWGG 1 cut(s) 1120
MvnI CGCG 5 cut(s) 732, 938, 1013, 1152, 1157
NciI CCSGG 5 cut(s) 569, 635, 636, 776, 890
NdeII GATC 3 cut(s) 447, 558, 969
NlaIII CATG 5 cut(s) 305, 772, 784, 829, 1036
NmuCI GTSAC 5 cut(s) 718, 893, 899, 1079, 1114
NspI RCATGY 2 cut(s) 784, 1036
PaeI GCATGC 1 cut(s) 1036
PaeR7I CTCGAG 1 cut(s) 920
PctI GAATGC 2 cut(s) 1145, 1404
PfeI GAWTC 1 cut(s) 1315
PflMI CCANNNNNTGG 1 cut(s) 481
PleI GAGTC 3 cut(s) 485, 896, 1205
PmaCI CACGTG 1 cut(s) 1215
PmlI CACGTG 1 cut(s) 1215
PpsI GAGTC 3 cut(s) 485, 896, 1205
Ppu21I YACGTR 2 cut(s) 481, 1215
PpuMI RGGWCCY 2 cut(s) 961, 1039
Psp124BI GAGCTC 1 cut(s) 1077
Psp5II RGGWCCY 2 cut(s) 961, 1039
Psp6I CCWGG 1 cut(s) 1118
PspCI CACGTG 1 cut(s) 1215
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 1 cut(s) 1118
PspOMI GGGCCC 3 cut(s) 821, 984, 1280
PspPPI RGGWCCY 2 cut(s) 961, 1039
PspXI VCTCGAGB 1 cut(s) 920
PvuII CAGCTG 1 cut(s) 844
RsaI GTAC 4 cut(s) 107, 662, 1251, 1336
RsaNI GTAC 4 cut(s) 106, 661, 1250, 1335
SacI GAGCTC 1 cut(s) 1077
Sau3AI GATC 3 cut(s) 447, 558, 969
SchI GAGTC 3 cut(s) 485, 896, 1206
ScrFI CCNGG 6 cut(s) 569, 635, 636, 776, 890, 1120
SduI GDGCHC 5 cut(s) 701, 825, 988, 1077, 1284
SexAI ACCWGGT 1 cut(s) 1118
SfaNI GCATC 2 cut(s) 318, 930
SfiI GGCCNNNNNGGCC 1 cut(s) 1014
Sfr274I CTCGAG 1 cut(s) 920
SinI GGWCC 4 cut(s) 514, 961, 1039, 1448
SlaI CTCGAG 1 cut(s) 920
SmaI CCCGGG 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
SpeI ACTAGT 1 cut(s) 367
SphI GCATGC 1 cut(s) 1036
Sse9I AATT 3 cut(s) 39, 277, 376
SspI AATATT 1 cut(s) 1390
SspMI CTAG 3 cut(s) 32, 368, 1462
SstI GAGCTC 1 cut(s) 1077
StyD4I CCNGG 6 cut(s) 567, 633, 634, 774, 888, 1118
StyI CCWWGG 1 cut(s) 1018
TaaI ACNGT 4 cut(s) 613, 718, 899, 1258
TaiI ACGT 9 cut(s) 111, 324, 483, 750, 1005, 1059, 1133, 1217, 1340
TaqI TCGA 3 cut(s) 564, 711, 921
TasI AATT 3 cut(s) 39, 277, 376
TauI GCSGC 8 cut(s) 63, 273, 624, 732, 765, 797, 852, 938
TfiI GAWTC 1 cut(s) 1315
TscAI CASTG 2 cut(s) 524, 542
TseFI GTSAC 5 cut(s) 718, 893, 899, 1079, 1114
Tsp45I GTSAC 5 cut(s) 718, 893, 899, 1079, 1114
TspDTI ATGAA 3 cut(s) 215, 290, 785
TspGWI ACGGA 4 cut(s) 401, 413, 1085, 1098
TspMI CCCGGG 1 cut(s) 634
TspRI CASTG 2 cut(s) 524, 542
Van91I CCANNNNNTGG 1 cut(s) 481
VpaK11BI GGWCC 4 cut(s) 514, 961, 1039, 1448
XceI RCATGY 2 cut(s) 784, 1036
XhoI CTCGAG 1 cut(s) 920
XmaI CCCGGG 1 cut(s) 634
XspI CTAG 3 cut(s) 32, 368, 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.