Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0014901

Physical Location & Seq

Genomic Coordinates Forward (+)

64601409 .. 64603392

Gene Structure 1984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG446600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGAGGGGATACTATTACTCTGCGTGGCTCTAGTCATAATTACAGGTTTCGGTATTAGCGGCACCGCCACCACCATCACCACTTACCCCGATTTCCAAGAGTTGCACGTCAAACAAACAATCGCTTCTGCTTCTGCTGCCACCAAGCTATACAAGGAAGAAGATGATGATGGTGGTAGCGAAAGCGGAGGAGCAAGATGGAAGGTGAAGGTGGTTCATAGAGATAAGATATTCCCGCAAGCGCCATACAACAAGAGCAGCGGCAGCAATTCACATCATCATCATCTGTTTCATGCACGCATCAACAGAGACGTGAAAAGGGTTGATAGCCTTATGCGTCGCCTCGGAAATAGTAAACTAGTCGTCAATTCCGCCACGGACTTTGGAACGGAGGTGGTTTCGGGGATGGCGCAAGGCAGCGGTGAATACTTTGTTAGGATCGGCGTTGGCAGCCCTCCCAGAAGCCAATACGTGGTCATTGACTCCGGCAGCGATATTGTGTGGGTCCAGTGTCAACCTTGTAGCCAGTGTTATCAGCAGTCCGACCCGATCTTCGACCCGGCACACTCTGCTTCTTACTCCGGCGTGTCTTGCACCTCCACCGTGTGTGGCCGCCTCCAGAACCCGGGCTGCCACGCAGGTCGGTGCCGGTACGAGGCTTCCTACGGCGACGGCTCCTACACGCAGGGCACTCTCGCCCTCGAAACAGTCACCTTCGGCCGCGCTCTCATCCGCAACGTGGCCATCGGCTGCGGCCACATGAACCGGGGCATGTTCGTCGGAGCCGCAGGTCTTTTGGGCCTCGGGAGTGGGCCCATGTCATTCGTCGGCCAGCTGGGCGGCCAGACTGGCGGTGCATTTTCCTACTGCTTGGTCAGCCGGGGTGACGTCGGCGACTCCACCACCGGCTCACTCGAGTTCGGGCGTGCCGCGATGCCCGTGGGTGCTGCGTGGGTCCCTCTGATCCGGAACCAACGGGCCCCGAGTTTCTACTACGTTGGCCTCGCGGGCCTTGGTGTCGGAGGCATGCGGGTCCCAATATCCGAAGACGTGTTCCGGCAGACGGAGCTCGGTGACGGAGGCGTTGTTATGGACACCGGAACGGCCGTCACCAGGTTCCCGACGTTGGCTTATCAGGCATTCCGCGACGCGTTTCTTCAGCAAACCGCAAACCTCCCTCGGGTCTCCGGAGTCTCCATCTTTGACACGTGTTATGACCTGAACGGCTTTGTGTCGGTTCGGGTACCGACCGTTTCCTTTTATTTCTCGGGCGGGCCCATCCTGACCCTTCCGGCAAGGAACTTTCTGATTCCGGTGGATGACAAGGGTACGTTTTGTTTGGCATTTGCTCCTTCCGCTGCGGGGCTTTCCATAATAGGGAATATTCAGCAAGAGGGCATTCAAATCTCATTTGATGGAGCTAATGGTTTTGTAGGATTTGGACCCAATGTCTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

485

Amino Acids

51.34

Weight (kDa)

6.78

Isoelectric Point (pI)

33.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	142 - 194	1e-07	Eukaryotic aspartyl protease
TAXi_N	PF14543	143 - 308	1.9e-47	Xylanase inhibitor N-terminal
Asp	PF00026	219 - 481	6.3e-08	Eukaryotic aspartyl protease
TAXi_C	PF14541	331 - 481	3.5e-31	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20015
fragaria_vesca	FvH4_4g37260
malus_domestica	MD00G1127200.v1.1 MD13G1002100.v1.1
prunus_persica	Prupe.1G350500_v2.0.a1
pyrus_communis	pycom13g00180 pycom16g00070
rosa_chinensis	RchiOBHm_Chr4g0447081
rosa_laevigata	RLG00000005607
rosa_roxburghii	Rroxscaffold_5G00387390
rosa_rugosa	Rorug04G0377800
rosa_samantha	Rh4AG439100 Rh4BG448100 Rh4CG465200 Rh4DG446600
rosa_wichuraiana	Rw4G037560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	891
Acc36I	ACCTGC	2 cut(s)	629, 779
Acc65I	GGTACC	1 cut(s)	1243
AccB1I	GGYRCC	3 cut(s)	62, 645, 1243
AccB7I	CCANNNNNTGG	1 cut(s)	472
AccII	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
AccIII	TCCGGA	2 cut(s)	966, 1187
AclWI	GGATC	2 cut(s)	446, 958
AcoI	YGGCCR	7 cut(s)	610, 718, 741, 754, 829, 841, 1104
AcuI	CTGAAG	1 cut(s)	1142
AcvI	CACGTG	1 cut(s)	1209
AcyI	GRCGYC	1 cut(s)	888
AdeI	CACNNNGTG	1 cut(s)	606
AfaI	GTAC	3 cut(s)	653, 1245, 1330
AflIII	ACRYGT	4 cut(s)	1050, 1149, 1206, 1208
AgsI	TTSAA	1 cut(s)	1403
AhdI	GACNNNNNGTC	1 cut(s)	1448
AhlI	ACTAGT	1 cut(s)	358
AjiI	CACGTC	3 cut(s)	109, 313, 1051
AjnI	CCWGG	1 cut(s)	1112
AluBI	AGCT	4 cut(s)	148, 835, 1069, 1421
AluI	AGCT	4 cut(s)	148, 835, 1069, 1421
Alw21I	GWGCWC	1 cut(s)	1071
Alw26I	GTCTC	3 cut(s)	303, 1189, 1198
AlwI	GGATC	2 cut(s)	446, 958
Ama87I	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
Aor13HI	TCCGGA	2 cut(s)	966, 1187
ApaI	GGGCCC	3 cut(s)	816, 982, 1278
ArsI	GACNNNNNNTTYG	2 cut(s)	536, 568
Asp718I	GGTACC	1 cut(s)	1243
AspLEI	GCGC	3 cut(s)	244, 412, 725
AsuC2I	CCSGG	5 cut(s)	560, 626, 627, 767, 881
AsuHPI	GGTGA	7 cut(s)	70, 217, 434, 703, 896, 1085, 1102
AvaI	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
AvaII	GGWCC	4 cut(s)	505, 955, 1033, 1442
BaeGI	GKGCMC	4 cut(s)	692, 816, 982, 1278
BalI	TGGCCA	1 cut(s)	743
BanI	GGYRCC	3 cut(s)	62, 645, 1243
BanII	GRGCYC	4 cut(s)	816, 982, 1071, 1278
BbrPI	CACGTG	1 cut(s)	1209
BbsI	GAAGAC	1 cut(s)	1053
Bbv12I	GWGCWC	1 cut(s)	1071
BccI	CCATC	8 cut(s)	83, 164, 192, 400, 752, 1205, 1286, 1409
BceAI	ACGGC	5 cut(s)	682, 688, 1091, 1119, 1240
BcgI	CGANNNNNNTGC	2 cut(s)	760, 794
BciT130I	CCWGG	1 cut(s)	1114
BciVI	GTATCC	1 cut(s)	3
BcnI	CCSGG	5 cut(s)	560, 626, 627, 767, 881
BcoDI	GTCTC	3 cut(s)	303, 1189, 1198
BcuI	ACTAGT	1 cut(s)	358
BfaI	CTAG	3 cut(s)	32, 359, 1456
BfoI	RGCGCY	1 cut(s)	245
BfuAI	ACCTGC	2 cut(s)	629, 779
BfuI	GTATCC	1 cut(s)	3
BglI	GCCNNNNNGGC	3 cut(s)	837, 849, 1008
Bme1390I	CCNGG	6 cut(s)	560, 626, 627, 767, 881, 1114
Bme18I	GGWCC	4 cut(s)	505, 955, 1033, 1442
BmeRI	GACNNNNNGTC	1 cut(s)	1448
BmeT110I	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
BmgBI	CACGTC	3 cut(s)	109, 313, 1051
BmrFI	CCNGG	6 cut(s)	560, 626, 627, 767, 881, 1114
BmsI	GCATC	2 cut(s)	309, 924
BpiI	GAAGAC	1 cut(s)	1053
BpmI	CTGGAG	1 cut(s)	602
BpuMI	CCSGG	5 cut(s)	560, 626, 627, 767, 881
BsaAI	YACGTR	2 cut(s)	472, 1209
BsaHI	GRCGYC	1 cut(s)	888
BsaI	GGTCTC	1 cut(s)	1189
BsaJI	CCNNGG	9 cut(s)	343, 375, 625, 766, 802, 880, 939, 1012, 1178
BsaWI	WCCGGW	4 cut(s)	966, 1097, 1187, 1312
BsaXI	ACNNNNNCTCC	2 cut(s)	1071, 1101
Bse118I	RCCGGY	2 cut(s)	648, 905
Bse1I	ACTGG	3 cut(s)	508, 526, 853
BseAI	TCCGGA	2 cut(s)	966, 1187
BseBI	CCWGG	1 cut(s)	1114
BseDI	CCNNGG	9 cut(s)	343, 375, 625, 766, 802, 880, 939, 1012, 1178
BseGI	GGATG	4 cut(s)	411, 729, 1278, 1324
BseNI	ACTGG	3 cut(s)	508, 526, 853
BseRI	GAGGAG	1 cut(s)	204
BseSI	GKGCMC	4 cut(s)	692, 816, 982, 1278
BseX3I	CGGCCG	2 cut(s)	718, 1104
BseYI	CCCAGC	1 cut(s)	835
Bsh1236I	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
Bsh1285I	CGRYCG	3 cut(s)	721, 1107, 1251
BshNI	GGYRCC	3 cut(s)	62, 645, 1243
BsiEI	CGRYCG	3 cut(s)	721, 1107, 1251
BsiHKAI	GWGCWC	1 cut(s)	1071
BsiHKCI	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
BslFI	GGGAC	2 cut(s)	941, 1019
BsmAI	GTCTC	3 cut(s)	303, 1189, 1198
BsmBI	CGTCTC	1 cut(s)	303
BsmFI	GGGAC	2 cut(s)	941, 1019
BsmI	GAATGC	2 cut(s)	1139, 1398
Bso31I	GGTCTC	1 cut(s)	1189
BsoBI	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
Bsp120I	GGGCCC	3 cut(s)	812, 978, 1274
Bsp1286I	GDGCHC	5 cut(s)	692, 816, 982, 1071, 1278
Bsp13I	TCCGGA	2 cut(s)	966, 1187
Bsp143I	GATC	3 cut(s)	438, 549, 963
BspEI	TCCGGA	2 cut(s)	966, 1187
BspFNI	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
BspMI	ACCTGC	2 cut(s)	629, 779
BspPI	GGATC	2 cut(s)	446, 958
BspT107I	GGYRCC	3 cut(s)	62, 645, 1243
BspTNI	GGTCTC	1 cut(s)	1189
BsrFI	RCCGGY	2 cut(s)	648, 905
BsrI	ACTGG	3 cut(s)	508, 526, 853
BssAI	RCCGGY	2 cut(s)	648, 905
BssECI	CCNNGG	9 cut(s)	343, 375, 625, 766, 802, 880, 939, 1012, 1178
BssMI	GATC	3 cut(s)	438, 549, 963
BssNI	GRCGYC	1 cut(s)	888
BssT1I	CCWWGG	1 cut(s)	1012
Bst2UI	CCWGG	1 cut(s)	1114
Bst4CI	ACNGT	3 cut(s)	604, 709, 1252
BstACI	GRCGYC	1 cut(s)	888
BstAPI	GCANNNNNTGC	1 cut(s)	569
BstBAI	YACGTR	2 cut(s)	472, 1209
BstC8I	GCNNGC	7 cut(s)	240, 298, 833, 927, 1009, 1028, 1274
BstDSI	CCRYGG	2 cut(s)	375, 939
BstF5I	GGATG	4 cut(s)	411, 729, 1278, 1324
BstFNI	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
BstH2I	RGCGCY	1 cut(s)	245
BstHHI	GCGC	3 cut(s)	244, 412, 725
BstKTI	GATC	3 cut(s)	441, 552, 966
BstMAI	GTCTC	3 cut(s)	303, 1189, 1198
BstMBI	GATC	3 cut(s)	438, 549, 963
BstMCI	CGRYCG	3 cut(s)	721, 1107, 1251
BstNI	CCWGG	1 cut(s)	1114
BstNSI	RCATGY	2 cut(s)	775, 1030
BstSCI	CCNGG	6 cut(s)	558, 624, 625, 765, 879, 1112
BstSLI	GKGCMC	4 cut(s)	692, 816, 982, 1278
BstUI	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
BstV2I	GAAGAC	1 cut(s)	1053
BstZI	CGGCCG	2 cut(s)	718, 1104
BsuI	GTATCC	1 cut(s)	3
BtgI	CCRYGG	2 cut(s)	375, 939
BtgZI	GCGATG	1 cut(s)	947
BtrI	CACGTC	3 cut(s)	109, 313, 1051
BtsCI	GGATG	4 cut(s)	411, 729, 1278, 1324
BtsIMutI	CAGTG	2 cut(s)	515, 533
BveI	ACCTGC	2 cut(s)	629, 779
Cac8I	GCNNGC	7 cut(s)	240, 298, 833, 927, 1009, 1028, 1274
CfoI	GCGC	3 cut(s)	244, 412, 725
Cfr10I	RCCGGY	2 cut(s)	648, 905
Cfr9I	CCCGGG	1 cut(s)	625
CseI	GACGC	2 cut(s)	326, 1157
CsiI	ACCWGGT	1 cut(s)	1112
Csp6I	GTAC	3 cut(s)	652, 1244, 1329
CspCI	CAANNNNNGTGG	2 cut(s)	364, 399
CviAII	CATG	5 cut(s)	293, 760, 772, 817, 1027
CviQI	GTAC	3 cut(s)	652, 1244, 1329
DpnI	GATC	3 cut(s)	440, 551, 965
DpnII	GATC	3 cut(s)	438, 549, 963
DraIII	CACNNNGTG	1 cut(s)	606
DriI	GACNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	7 cut(s)	610, 718, 741, 754, 829, 841, 1104
EagI	CGGCCG	2 cut(s)	718, 1104
Eam1105I	GACNNNNNGTC	1 cut(s)	1448
EciI	GGCGGA	1 cut(s)	361
Ecl136II	GAGCTC	1 cut(s)	1069
EclXI	CGGCCG	2 cut(s)	718, 1104
Eco130I	CCWWGG	1 cut(s)	1012
Eco24I	GRGCYC	4 cut(s)	816, 982, 1071, 1278
Eco31I	GGTCTC	1 cut(s)	1189
Eco47I	GGWCC	4 cut(s)	505, 955, 1033, 1442
Eco52I	CGGCCG	2 cut(s)	718, 1104
Eco53kI	GAGCTC	1 cut(s)	1069
Eco57I	CTGAAG	1 cut(s)	1142
Eco72I	CACGTG	1 cut(s)	1209
Eco88I	CYCGRG	6 cut(s)	625, 803, 914, 982, 1179, 1267
EcoICRI	GAGCTC	1 cut(s)	1069
EcoO109I	RGGNCCY	3 cut(s)	955, 979, 1033
EcoRII	CCWGG	1 cut(s)	1112
EcoT14I	CCWWGG	1 cut(s)	1012
EcoT38I	GRGCYC	4 cut(s)	816, 982, 1071, 1278
ErhI	CCWWGG	1 cut(s)	1012
Esp3I	CGTCTC	1 cut(s)	303
FaeI	CATG	5 cut(s)	296, 763, 775, 820, 1030
FaqI	GGGAC	2 cut(s)	941, 1019
FatI	CATG	5 cut(s)	292, 759, 771, 816, 1026
FauI	CCCGC	5 cut(s)	243, 1000, 1023, 1265, 1354
FokI	GGATG	4 cut(s)	418, 716, 1265, 1331
FriOI	GRGCYC	4 cut(s)	816, 982, 1071, 1278
FspBI	CTAG	3 cut(s)	32, 359, 1456
GlaI	GCGC	3 cut(s)	243, 411, 724
GsaI	CCCAGC	1 cut(s)	839
GsuI	CTGGAG	1 cut(s)	602
HaeII	RGCGCY	1 cut(s)	245
HgaI	GACGC	2 cut(s)	326, 1157
HhaI	GCGC	3 cut(s)	244, 412, 725
Hin1I	GRCGYC	1 cut(s)	888
Hin1II	CATG	5 cut(s)	296, 763, 775, 820, 1030
Hin6I	GCGC	3 cut(s)	242, 410, 723
HinP1I	GCGC	3 cut(s)	242, 410, 723
HincII	GTYRAC	1 cut(s)	515
HindII	GTYRAC	1 cut(s)	515
HinfI	GANTC	4 cut(s)	482, 896, 1191, 1309
HphI	GGTGA	7 cut(s)	70, 217, 434, 703, 896, 1085, 1102
Hpy166II	GTNNAC	2 cut(s)	356, 515
Hpy188I	TCNGA	7 cut(s)	347, 544, 782, 963, 1022, 1045, 1308
Hpy188III	TCNNGA	6 cut(s)	619, 805, 967, 1120, 1188, 1282
Hpy8I	GTNNAC	2 cut(s)	356, 515
Hpy99I	CGWCG	7 cut(s)	342, 674, 782, 830, 893, 1126, 1151
HpyAV	CCTTC	5 cut(s)	196, 202, 724, 1298, 1362
HpyCH4III	ACNGT	3 cut(s)	604, 709, 1252
HpyCH4V	TGCA	4 cut(s)	106, 296, 594, 857
Hsp92I	GRCGYC	1 cut(s)	888
Hsp92II	CATG	5 cut(s)	296, 763, 775, 820, 1030
HspAI	GCGC	3 cut(s)	242, 410, 723
KflI	GGGWCCC	2 cut(s)	955, 1033
Kpn2I	TCCGGA	2 cut(s)	966, 1187
KpnI	GGTACC	1 cut(s)	1247
Kzo9I	GATC	3 cut(s)	438, 549, 963
LmnI	GCTCC	6 cut(s)	191, 680, 782, 1066, 1354, 1418
LweI	GCATC	2 cut(s)	309, 924
MabI	ACCWGGT	1 cut(s)	1112
MaeI	CTAG	3 cut(s)	32, 359, 1456
MaeIII	GTNAC	4 cut(s)	709, 884, 1073, 1108
MalI	GATC	3 cut(s)	440, 551, 965
MboI	GATC	3 cut(s)	438, 549, 963
MboII	GAAGA	5 cut(s)	170, 173, 544, 1058, 1148
MhlI	GDGCHC	5 cut(s)	692, 816, 982, 1071, 1278
MlsI	TGGCCA	1 cut(s)	743
MluCI	AATT	3 cut(s)	39, 268, 367
MluI	ACGCGT	1 cut(s)	1149
MluNI	TGGCCA	1 cut(s)	743
MlyI	GAGTC	3 cut(s)	476, 890, 1200
MmeI	TCCRAC	3 cut(s)	567, 760, 1000
Mox20I	TGGCCA	1 cut(s)	743
MroI	TCCGGA	2 cut(s)	966, 1187
MscI	TGGCCA	1 cut(s)	743
Msp20I	TGGCCA	1 cut(s)	743
MspA1I	CMGCKG	4 cut(s)	261, 420, 835, 1358
MspR9I	CCNGG	6 cut(s)	560, 626, 627, 767, 881, 1114
Mva1269I	GAATGC	2 cut(s)	1139, 1398
MvaI	CCWGG	1 cut(s)	1114
MvnI	CGCG	5 cut(s)	723, 932, 1007, 1146, 1151
NciI	CCSGG	5 cut(s)	560, 626, 627, 767, 881
NdeII	GATC	3 cut(s)	438, 549, 963
NlaIII	CATG	5 cut(s)	296, 763, 775, 820, 1030
NmuCI	GTSAC	4 cut(s)	709, 884, 1073, 1108
NspI	RCATGY	2 cut(s)	775, 1030
PaeI	GCATGC	1 cut(s)	1030
PaeR7I	CTCGAG	1 cut(s)	914
PctI	GAATGC	2 cut(s)	1139, 1398
PfeI	GAWTC	1 cut(s)	1309
PflMI	CCANNNNNTGG	1 cut(s)	472
PleI	GAGTC	3 cut(s)	476, 890, 1199
PmaCI	CACGTG	1 cut(s)	1209
PmlI	CACGTG	1 cut(s)	1209
PpsI	GAGTC	3 cut(s)	476, 890, 1199
Ppu21I	YACGTR	2 cut(s)	472, 1209
PpuMI	RGGWCCY	2 cut(s)	955, 1033
Psp124BI	GAGCTC	1 cut(s)	1071
Psp5II	RGGWCCY	2 cut(s)	955, 1033
Psp6I	CCWGG	1 cut(s)	1112
PspCI	CACGTG	1 cut(s)	1209
PspFI	CCCAGC	1 cut(s)	835
PspGI	CCWGG	1 cut(s)	1112
PspOMI	GGGCCC	3 cut(s)	812, 978, 1274
PspPPI	RGGWCCY	2 cut(s)	955, 1033
PspXI	VCTCGAGB	1 cut(s)	914
PvuII	CAGCTG	1 cut(s)	835
RsaI	GTAC	3 cut(s)	653, 1245, 1330
RsaNI	GTAC	3 cut(s)	652, 1244, 1329
SacI	GAGCTC	1 cut(s)	1071
Sau3AI	GATC	3 cut(s)	438, 549, 963
SchI	GAGTC	3 cut(s)	476, 890, 1200
ScrFI	CCNGG	6 cut(s)	560, 626, 627, 767, 881, 1114
SduI	GDGCHC	5 cut(s)	692, 816, 982, 1071, 1278
SexAI	ACCWGGT	1 cut(s)	1112
SfaNI	GCATC	2 cut(s)	309, 924
SfiI	GGCCNNNNNGGCC	1 cut(s)	1008
Sfr274I	CTCGAG	1 cut(s)	914
SinI	GGWCC	4 cut(s)	505, 955, 1033, 1442
SlaI	CTCGAG	1 cut(s)	914
SmaI	CCCGGG	1 cut(s)	627
SmlI	CTYRAG	1 cut(s)	914
SmoI	CTYRAG	1 cut(s)	914
SpeI	ACTAGT	1 cut(s)	358
SphI	GCATGC	1 cut(s)	1030
Sse9I	AATT	3 cut(s)	39, 268, 367
SspI	AATATT	1 cut(s)	1384
SspMI	CTAG	3 cut(s)	32, 359, 1456
SstI	GAGCTC	1 cut(s)	1071
StyD4I	CCNGG	6 cut(s)	558, 624, 625, 765, 879, 1112
StyI	CCWWGG	1 cut(s)	1012
TaaI	ACNGT	3 cut(s)	604, 709, 1252
TaqI	TCGA	3 cut(s)	555, 702, 915
TasI	AATT	3 cut(s)	39, 268, 367
TauI	GCSGC	8 cut(s)	63, 264, 615, 723, 756, 788, 843, 932
TfiI	GAWTC	1 cut(s)	1309
TscAI	CASTG	2 cut(s)	515, 533
TseFI	GTSAC	4 cut(s)	709, 884, 1073, 1108
Tsp45I	GTSAC	4 cut(s)	709, 884, 1073, 1108
TspDTI	ATGAA	3 cut(s)	206, 281, 776
TspGWI	ACGGA	4 cut(s)	392, 404, 1079, 1092
TspMI	CCCGGG	1 cut(s)	625
TspRI	CASTG	2 cut(s)	515, 533
Van91I	CCANNNNNTGG	1 cut(s)	472
VpaK11BI	GGWCC	4 cut(s)	505, 955, 1033, 1442
XceI	RCATGY	2 cut(s)	775, 1030
XhoI	CTCGAG	1 cut(s)	914
XmaI	CCCGGG	1 cut(s)	625
XspI	CTAG	3 cut(s)	32, 359, 1456
ZraI	GACGTC	1 cut(s)	889

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.