Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014306

Physical Location & Seq

Genomic Coordinates Reverse (-)

7202748 .. 7208547

Gene Structure 5800 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G20630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2394 bp

ATGGAGCTCCTCCGATCCAACTTGTCTAGGGTTCAGATCCCTGAACCTACTCATCGCATCTACAAGCATGAGTGTTGCATCTCCTTCGATACTCCGAGATCCGAAGGAGGATTGTTCGTTGATATGAATAGTTTTCTTGCTTTCGGGAAGGATTATGTTTCTTGGAACTATGAGAAGACTGGAAACCCTGTTTATCTTCATATTAAGCAGACTAGGAAGTCTATTCCCGAGGATCGGCCTCTCAAGAAACCGACTCTGCTCGCTATAGGTGTTGATGGAGGCTTTGATAACAATGAGCCAGAGTACGAGGAGTCTTATAGCATAGTCATACTTCCGGATTTTGTTTCACTCCCGTTTCCTTCTGTTGAGCTACCAGAGAAGGTGAGGATTGCTGTCGATACTGTAGTGAATGCCGTTGGTGCTGAGCGGAAAGAGCAAGTTGCAGCTTGGACAGCTGAGAAAAAGTTAATTAGTGAACATGCATTGACACTGCAGCAAATCAAGAGTGGCATTGTCATTCCTCCCTCTGGTTGGAAATGTTCTAAGTGTGATAAGACGGAGAATCTCTGGCTTAATCTTACCGATGGAATGATTCTCTGTGGAAGAAAAAACTGGGATGGAACTGGTGGAAACAACCATGCTGTTGAGCACTACAAAGAGACAGCCTACCCTCTTGCTGTAAAGCTTGGAACTATCACTGCTGACTTAGAAGCAGCAGATGTTTATTCCTATCCGGAAGATGACAGTGTTTTGGACCCGCTCCTGGCTGAGCATCTGGCCCATTTTGGAATTGACTTCTCGTCGATGCAGAAGACTGAAATGACAACTGCTGAAAGAGAACTTGATCAAAATACAAATTTTGATTGGAACCGTATACAAGAGAGTGGAAAAGAATTGGTGCCAGTTTTTGGACCTGGATATACCGGACTTGTCAATCTTGGGAACAGTTGCTACTTGGCAGCTACGATGCAGATTGTTTTCTCTACCCATTCATTTATTTCAAGATACTTTTCACATCAGAGCTTAAAGATGGCTTTTGAGATGGCTCCTGCTGATCCAACTTTGGACCTCAATATGCAATTAACAAAGCTTGGACACGGCTTACTATCTGGGAAGTACTCAATGCCTGCAACTCAGAAGGATGCTACAACTGGAGATCCTAGACAAGAAGGAATACCTCCTCGCATGTTCAAAAACGTAATTGCCGCGAGCCACGCAGAATTCTCTTCTATGAGACAGCAGGATGCTCTCGACTTTTTCCTCCATCTGGTAGGCAAGGTTGAGCGTGCTAGCAACACGACACCAGATTTAGACCCCTCGAGGAGCTTCAAGTTTGGAATAGAAGAGAAGATCCTTTGTCCGTCTGGAAAGGTTGGATATAATAAGAGGGAAGACTGTATTCTTTCTTTGAACATTCCGCTACATGAGGCAACTAATAAAGATGAATTAGAAGCCTTTCACAAGCAAAAAGCAGGAAAAGGATTGGAAGAGAATGATATGAGGTCAAGTGATGAAATAGTACGCCCACGAGTTCCTTTAGAAGCCTGTCTAGCAAATTTTGCATCATCAGAGCCGATTGAAGACTACTATAGCTCTGCTTTGAAGGGAATGACAACAGCTATCAAGACAACTGGTTTGACATCTTTCCCAGATTATTTGGTCTTGCACATGCGGAAATTTGTTATGGAGGAAGGCTGGGTGCCAAAGAAACTTGATGTATACATTGATGTTCCGGATGTTATTGATATCAGCCACATGCGTAGCAAAGGACTCCAACCTGGGGAAGAACTGTTGCCAGATGGCGTTCCAGAAGAAGTGATGGAATCAGCGCAGCCCGTGGCAAATGAGGAGATAGTTGCACAGCTAGTCTCAATGGGATTTAGCCAGCTTCACTGCCAGAAAGCTGCCATAAATACTTCCAATGCTGGGGTTGAAGAGGCAATGAACTGGTTACTTTCTCACATGGATGATCCAGACATCGATGCACCAATCTCCCACCAGACATCTGACATTGATCAATCAAGCGTTGATACCTTACTCTCCTTTGGTTTTGCTGAAGATGTTGCTCGGAAGGCACTAAAAGCCTCGGGAGGAGACATTGAGAAAGCAACAGACTGGGTATTCAACAACCCTAATGCATCTGTTTCAGACATGGATGTATCCTCTAGCAATTCAGCGCAGACTCCGGCTCAAAGTGGATTACCAGATGGAGGAGGGAAATACAAGCTGTTTGGAATAGTAAGTCACATGGGAACATCAGTGCACTGTGGTCATTACGTGGCTCACATATTGAAAGAAGGCCGCTGGGTAATTTTCAATGACGACAAAGTTGGTATCTCGACTGATCCTCCTAAAGACATGGGTTATGTCTACTTCTTTCAGCGGCTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0016049 GO:0016579 GO:0016787 GO:0019538 GO:0019783 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0036459 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070011 GO:0070646 GO:0070647 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0101005 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA UCH zf-UBP

Protein Analysis

797

Amino Acids

88.37

Weight (kDa)

5.06

Isoelectric Point (pI)

37.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP_var	PF17807	11 - 73	8.2e-22	Variant UBP zinc finger
zf-UBP	PF02148	180 - 253	1e-18	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	308 - 793	1.4e-43	Ubiquitin carboxyl-terminal hydrolase
UBA_7	PF22562	615 - 662	1.9e-21	UBA-like domain
UBA	PF00627	615 - 651	8.7e-09	UBA/TS-N domain
UBA	PF00627	671 - 706	2.1e-09	UBA/TS-N domain
UBA_7	PF22562	672 - 714	9.1e-11	UBA-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20630
fragaria_vesca	FvH4_3g21160 FvH4_3g21160
malus_domestica	MD03G1217200.v1.1 MD11G1234900.v1.1
prunus_persica	Prupe.4G192200_v2.0.a1
pyrus_communis	pycom11g20650
rosa_chinensis	RchiOBHm_Chr5g0036241
rosa_laevigata	RLG00000033681
rosa_multiflora	Rmu_sc0000247.1_g000007
rosa_roxburghii	Rroxscaffold_1G00044540
rosa_rugosa	Rorug05G0154300
rosa_samantha	Rh5AG248000 Rh5BG248600 Rh5CG279900 Rh5DG256900
rosa_wichuraiana	Rw5G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	217
AbsI	CCTCGAGG	1 cut(s)	1318
AccB1I	GGYRCC	2 cut(s)	898, 1699
AccB7I	CCANNNNNTGG	1 cut(s)	908
AccBSI	CCGCTC	2 cut(s)	427, 760
AccI	GTMKAC	3 cut(s)	874, 1719, 2370
AccII	CGCG	1 cut(s)	1208
AccIII	TCCGGA	3 cut(s)	334, 733, 1732
AciI	CCGC	7 cut(s)	427, 758, 1206, 1418, 1672, 2302, 2383
AclWI	GGATC	9 cut(s)	9, 31, 93, 240, 1049, 1151, 1345, 1964, 2339
AcsI	RAATTY	4 cut(s)	856, 1220, 1555, 1676
AcuI	CTGAAG	1 cut(s)	2076
AfaI	GTAC	3 cut(s)	305, 1118, 1521
AfiI	CCNNNNNNNGG	9 cut(s)	234, 527, 531, 763, 908, 1267, 1780, 1926, 2210
AhdI	GACNNNNNGTC	1 cut(s)	799
AjnI	CCWGG	3 cut(s)	762, 913, 1777
Alw21I	GWGCWC	3 cut(s)	9, 651, 2265
Alw26I	GTCTC	4 cut(s)	653, 1228, 1873, 2088
Alw44I	GTGCAC	1 cut(s)	2261
AlwI	GGATC	9 cut(s)	9, 31, 93, 240, 1049, 1151, 1345, 1964, 2339
Ama87I	CYCGRG	3 cut(s)	227, 1318, 2086
Aor13HI	TCCGGA	3 cut(s)	334, 733, 1732
AoxI	GGCC	3 cut(s)	236, 777, 2299
ApaLI	GTGCAC	1 cut(s)	2261
ApeKI	GCWGC	6 cut(s)	443, 493, 713, 959, 1831, 1904
ApoI	RAATTY	4 cut(s)	856, 1220, 1555, 1676
ArsI	GACNNNNNNTTYG	2 cut(s)	1618, 1650
Asp700I	GAANNNNTTC	1 cut(s)	1455
AspLEI	GCGC	2 cut(s)	1831, 2179
AspS9I	GGNCC	4 cut(s)	754, 778, 911, 1066
AsuHPI	GGTGA	1 cut(s)	394
AsuNHI	GCTAGC	1 cut(s)	1289
AvaI	CYCGRG	3 cut(s)	227, 1318, 2086
AvaII	GGWCC	3 cut(s)	754, 911, 1066
BaeGI	GKGCMC	1 cut(s)	2265
BaeI	ACNNNNGTAYC	2 cut(s)	390, 423
BanI	GGYRCC	2 cut(s)	898, 1699
BanII	GRGCYC	1 cut(s)	9
BauI	CACGAG	1 cut(s)	1527
BbsI	GAAGAC	4 cut(s)	182, 818, 1398, 1587
Bbv12I	GWGCWC	3 cut(s)	9, 651, 2265
BbvI	GCAGC	6 cut(s)	455, 505, 725, 971, 1843, 1891
BccI	CCATC	9 cut(s)	269, 578, 611, 1024, 1036, 1272, 1793, 1813, 2201
BceAI	ACGGC	2 cut(s)	398, 1114
BcgI	CGANNNNNNTGC	2 cut(s)	67, 101
BciT130I	CCWGG	3 cut(s)	764, 915, 1779
BciVI	GTATCC	1 cut(s)	2170
BclI	TGATCA	2 cut(s)	844, 2014
BcoDI	GTCTC	4 cut(s)	653, 1228, 1873, 2088
BfaI	CTAG	7 cut(s)	27, 213, 1161, 1290, 1550, 1865, 2166
BfmI	CTRYAG	4 cut(s)	264, 402, 491, 1588
BfuI	GTATCC	1 cut(s)	2170
BisI	GCNGC	9 cut(s)	444, 494, 714, 960, 1206, 1832, 1905, 2302, 2384
BlpI	GCTNAGC	2 cut(s)	423, 768
BlsI	GCNGC	9 cut(s)	445, 495, 715, 961, 1207, 1833, 1906, 2303, 2385
BmcAI	AGTACT	1 cut(s)	1118
Bme1390I	CCNGG	3 cut(s)	764, 915, 1779
Bme18I	GGWCC	3 cut(s)	754, 911, 1066
BmeRI	GACNNNNNGTC	1 cut(s)	799
BmeT110I	CYCGRG	3 cut(s)	227, 1318, 2086
BmgT120I	GGNCC	4 cut(s)	754, 778, 911, 1066
BmiI	GGNNCC	5 cut(s)	756, 869, 900, 1047, 1701
BmrFI	CCNGG	3 cut(s)	764, 915, 1779
BmrI	ACTGGG	2 cut(s)	622, 2125
BmtI	GCTAGC	1 cut(s)	1293
BmuI	ACTGGG	2 cut(s)	622, 2125
BpiI	GAAGAC	4 cut(s)	182, 818, 1398, 1587
BpmI	CTGGAG	1 cut(s)	1173
Bpu1102I	GCTNAGC	2 cut(s)	423, 768
BpuEI	CTTGAG	1 cut(s)	227
Bsa29I	ATCGAT	1 cut(s)	1980
BsaAI	YACGTR	1 cut(s)	2278
BsaJI	CCNNGG	4 cut(s)	228, 1778, 1836, 2085
BsaWI	WCCGGW	4 cut(s)	334, 733, 923, 1732
BsaXI	ACNNNNNCTCC	2 cut(s)	2332, 2362
Bsc4I	CCNNNNNNNGG	9 cut(s)	234, 527, 531, 763, 908, 1267, 1780, 1926, 2210
Bse1I	ACTGG	8 cut(s)	184, 617, 628, 902, 1156, 1636, 1952, 2120
Bse3DI	GCAATG	1 cut(s)	1947
BseAI	TCCGGA	3 cut(s)	334, 733, 1732
BseBI	CCWGG	3 cut(s)	764, 915, 1779
BseCI	ATCGAT	1 cut(s)	1980
BseDI	CCNNGG	4 cut(s)	228, 1778, 1836, 2085
BseGI	GGATG	6 cut(s)	622, 1147, 1249, 1741, 1972, 2161
BseLI	CCNNNNNNNGG	9 cut(s)	234, 527, 531, 763, 908, 1267, 1780, 1926, 2210
BseMI	GCAATG	1 cut(s)	1947
BseMII	CTCAG	4 cut(s)	414, 447, 759, 1148
BseNI	ACTGG	8 cut(s)	184, 617, 628, 902, 1156, 1636, 1952, 2120
BseRI	GAGGAG	6 cut(s)	323, 1170, 1336, 1862, 2106, 2226
BseSI	GKGCMC	1 cut(s)	2265
BseXI	GCAGC	6 cut(s)	455, 505, 725, 971, 1843, 1891
BseYI	CCCAGC	3 cut(s)	1695, 1925, 2304
Bsh1236I	CGCG	1 cut(s)	1208
BshFI	GGCC	3 cut(s)	238, 779, 2301
BshNI	GGYRCC	2 cut(s)	898, 1699
BshVI	ATCGAT	1 cut(s)	1980
BsiHKAI	GWGCWC	3 cut(s)	9, 651, 2265
BsiHKCI	CYCGRG	3 cut(s)	227, 1318, 2086
BsiSI	CCGG	5 cut(s)	335, 734, 924, 1733, 2186
BslI	CCNNNNNNNGG	9 cut(s)	234, 527, 531, 763, 908, 1267, 1780, 1926, 2210
BsmAI	GTCTC	4 cut(s)	653, 1228, 1873, 2088
BsmI	GAATGC	1 cut(s)	415
BsnI	GGCC	3 cut(s)	238, 779, 2301
BsoBI	CYCGRG	3 cut(s)	227, 1318, 2086
Bsp1286I	GDGCHC	3 cut(s)	9, 651, 2265
Bsp13I	TCCGGA	3 cut(s)	334, 733, 1732
Bsp1720I	GCTNAGC	2 cut(s)	423, 768
BspACI	CCGC	7 cut(s)	427, 758, 1206, 1418, 1672, 2302, 2383
BspANI	GGCC	3 cut(s)	238, 779, 2301
BspCNI	CTCAG	4 cut(s)	415, 448, 760, 1147
BspDI	ATCGAT	1 cut(s)	1980
BspEI	TCCGGA	3 cut(s)	334, 733, 1732
BspFNI	CGCG	1 cut(s)	1208
BspLI	GGNNCC	5 cut(s)	756, 869, 900, 1047, 1701
BspMAI	CTGCAG	1 cut(s)	495
BspOI	GCTAGC	1 cut(s)	1293
BspPI	GGATC	9 cut(s)	9, 31, 93, 240, 1049, 1151, 1345, 1964, 2339
BspT107I	GGYRCC	2 cut(s)	898, 1699
BsrBI	CCGCTC	2 cut(s)	427, 760
BsrDI	GCAATG	1 cut(s)	1947
BsrI	ACTGG	8 cut(s)	184, 617, 628, 902, 1156, 1636, 1952, 2120
BssECI	CCNNGG	4 cut(s)	228, 1778, 1836, 2085
BssNAI	GTATAC	2 cut(s)	875, 1720
BssSI	CACGAG	1 cut(s)	1527
Bst1107I	GTATAC	2 cut(s)	875, 1720
Bst2BI	CACGAG	1 cut(s)	1527
Bst2UI	CCWGG	3 cut(s)	764, 915, 1779
Bst4CI	ACNGT	7 cut(s)	403, 746, 872, 947, 1397, 1791, 2267
Bst6I	CTCTTC	4 cut(s)	1231, 1338, 1482, 1929
BstAPI	GCANNNNNTGC	1 cut(s)	1559
BstBAI	YACGTR	1 cut(s)	2278
BstC8I	GCNNGC	6 cut(s)	261, 1128, 1210, 1287, 1291, 1886
BstDEI	CTNAG	6 cut(s)	423, 456, 543, 706, 768, 1134
BstDSI	CCRYGG	1 cut(s)	1836
BstF5I	GGATG	6 cut(s)	622, 1147, 1249, 1741, 1972, 2161
BstFNI	CGCG	1 cut(s)	1208
BstHHI	GCGC	2 cut(s)	1831, 2179
BstMAI	GTCTC	4 cut(s)	653, 1228, 1873, 2088
BstMWI	GCNNNNNNNGC	8 cut(s)	419, 433, 452, 1214, 1550, 1559, 2072, 2081
BstNI	CCWGG	3 cut(s)	764, 915, 1779
BstNSI	RCATGY	4 cut(s)	482, 1189, 1672, 1759
BstSCI	CCNGG	3 cut(s)	762, 913, 1777
BstSFI	CTRYAG	4 cut(s)	264, 402, 491, 1588
BstSLI	GKGCMC	1 cut(s)	2265
BstUI	CGCG	1 cut(s)	1208
BstV1I	GCAGC	6 cut(s)	455, 505, 725, 971, 1843, 1891
BstV2I	GAAGAC	4 cut(s)	182, 818, 1398, 1587
BstX2I	RGATCY	4 cut(s)	36, 98, 1156, 1350
BstYI	RGATCY	4 cut(s)	36, 98, 1156, 1350
BstZ17I	GTATAC	2 cut(s)	875, 1720
Bsu15I	ATCGAT	1 cut(s)	1980
BsuI	GTATCC	1 cut(s)	2170
BsuRI	GGCC	3 cut(s)	238, 779, 2301
BsuTUI	ATCGAT	1 cut(s)	1980
BtgI	CCRYGG	1 cut(s)	1836
BtgZI	GCGATG	1 cut(s)	38
BtsCI	GGATG	6 cut(s)	622, 1147, 1249, 1741, 1972, 2161
BtsI	GCAGTG	3 cut(s)	488, 696, 1891
BtsIMutI	CAGTG	6 cut(s)	488, 696, 751, 1891, 2263, 2265
Cac8I	GCNNGC	6 cut(s)	261, 1128, 1210, 1287, 1291, 1886
CfoI	GCGC	2 cut(s)	1831, 2179
Cfr13I	GGNCC	4 cut(s)	754, 778, 911, 1066
ClaI	ATCGAT	1 cut(s)	1980
Csp6I	GTAC	3 cut(s)	304, 1117, 1520
CviQI	GTAC	3 cut(s)	304, 1117, 1520
DdeI	CTNAG	6 cut(s)	423, 456, 543, 706, 768, 1134
DrdI	GACNNNNNNGTC	1 cut(s)	217
DriI	GACNNNNNGTC	1 cut(s)	799
DseDI	GACNNNNNNGTC	1 cut(s)	217
Eam1104I	CTCTTC	4 cut(s)	1231, 1338, 1482, 1929
Eam1105I	GACNNNNNGTC	1 cut(s)	799
EarI	CTCTTC	4 cut(s)	1231, 1338, 1482, 1929
Ecl136II	GAGCTC	1 cut(s)	7
Eco24I	GRGCYC	1 cut(s)	9
Eco32I	GATATC	1 cut(s)	1747
Eco47I	GGWCC	3 cut(s)	754, 911, 1066
Eco53kI	GAGCTC	1 cut(s)	7
Eco57I	CTGAAG	1 cut(s)	2076
Eco88I	CYCGRG	3 cut(s)	227, 1318, 2086
EcoICRI	GAGCTC	1 cut(s)	7
EcoRI	GAATTC	1 cut(s)	1220
EcoRII	CCWGG	3 cut(s)	762, 913, 1777
EcoRV	GATATC	1 cut(s)	1747
EcoT22I	ATGCAT	2 cut(s)	484, 2140
EcoT38I	GRGCYC	1 cut(s)	9
FauI	CCCGC	1 cut(s)	765
FbaI	TGATCA	2 cut(s)	844, 2014
FblI	GTMKAC	3 cut(s)	874, 1719, 2370
Fnu4HI	GCNGC	9 cut(s)	444, 494, 714, 960, 1206, 1832, 1905, 2302, 2384
FokI	GGATG	6 cut(s)	629, 1154, 1256, 1748, 1979, 2168
FriOI	GRGCYC	1 cut(s)	9
Fsp4HI	GCNGC	9 cut(s)	444, 494, 714, 960, 1206, 1832, 1905, 2302, 2384
FspBI	CTAG	7 cut(s)	27, 213, 1161, 1290, 1550, 1865, 2166
GlaI	GCGC	2 cut(s)	1830, 2178
GluI	GCNGC	9 cut(s)	444, 494, 714, 960, 1206, 1832, 1905, 2302, 2384
GsaI	CCCAGC	3 cut(s)	1699, 1929, 2308
GsuI	CTGGAG	1 cut(s)	1173
HaeIII	GGCC	3 cut(s)	238, 779, 2301
HapII	CCGG	5 cut(s)	335, 734, 924, 1733, 2186
HhaI	GCGC	2 cut(s)	1831, 2179
Hin6I	GCGC	2 cut(s)	1829, 2177
HinP1I	GCGC	2 cut(s)	1829, 2177
HindIII	AAGCTT	2 cut(s)	683, 1088
HinfI	GANTC	7 cut(s)	253, 311, 562, 592, 1770, 1823, 2182
HpaII	CCGG	5 cut(s)	335, 734, 924, 1733, 2186
HphI	GGTGA	1 cut(s)	394
Hpy166II	GTNNAC	5 cut(s)	476, 875, 1720, 2263, 2371
Hpy8I	GTNNAC	5 cut(s)	476, 875, 1720, 2263, 2371
Hpy99I	CGWCG	1 cut(s)	805
HpyCH4III	ACNGT	7 cut(s)	403, 746, 872, 947, 1397, 1791, 2267
HpyCH4IV	ACGT	2 cut(s)	1197, 2277
HpyF10VI	GCNNNNNNNGC	8 cut(s)	419, 433, 452, 1214, 1550, 1559, 2072, 2081
HpyF3I	CTNAG	6 cut(s)	423, 456, 543, 706, 768, 1134
HpySE526I	ACGT	2 cut(s)	1197, 2277
HspAI	GCGC	2 cut(s)	1829, 2177
Kpn2I	TCCGGA	3 cut(s)	334, 733, 1732
Ksp22I	TGATCA	2 cut(s)	844, 2014
LmnI	GCTCC	5 cut(s)	4, 12, 765, 1051, 1323
Lsp1109I	GCAGC	6 cut(s)	455, 505, 725, 971, 1843, 1891
MaeI	CTAG	7 cut(s)	27, 213, 1161, 1290, 1550, 1865, 2166
MaeII	ACGT	2 cut(s)	1197, 2277
MaeIII	GTNAC	2 cut(s)	1950, 2243
MbiI	CCGCTC	2 cut(s)	427, 760
MflI	RGATCY	4 cut(s)	36, 98, 1156, 1350
MhlI	GDGCHC	3 cut(s)	9, 651, 2265
MlyI	GAGTC	4 cut(s)	247, 320, 1764, 2176
MmeI	TCCRAC	5 cut(s)	42, 512, 1082, 1354, 1798
Mph1103I	ATGCAT	2 cut(s)	484, 2140
MroI	TCCGGA	3 cut(s)	334, 733, 1732
MroXI	GAANNNNTTC	1 cut(s)	1455
MseI	TTAA	5 cut(s)	204, 467, 573, 1025, 1082
MslI	CAYNNNNRTG	1 cut(s)	1965
MspA1I	CMGCKG	3 cut(s)	455, 2304, 2383
MspI	CCGG	5 cut(s)	335, 734, 924, 1733, 2186
MspR9I	CCNGG	3 cut(s)	764, 915, 1779
Mva1269I	GAATGC	1 cut(s)	415
MvaI	CCWGG	3 cut(s)	764, 915, 1779
MvnI	CGCG	1 cut(s)	1208
MwoI	GCNNNNNNNGC	8 cut(s)	419, 433, 452, 1214, 1550, 1559, 2072, 2081
NheI	GCTAGC	1 cut(s)	1289
NlaIV	GGNNCC	5 cut(s)	756, 869, 900, 1047, 1701
NmuCI	GTSAC	1 cut(s)	2243
NsiI	ATGCAT	2 cut(s)	484, 2140
NspI	RCATGY	4 cut(s)	482, 1189, 1672, 1759
PaeR7I	CTCGAG	1 cut(s)	1318
PctI	GAATGC	1 cut(s)	415
PdmI	GAANNNNTTC	1 cut(s)	1455
PfeI	GAWTC	3 cut(s)	562, 592, 1823
PflMI	CCANNNNNTGG	1 cut(s)	908
PkrI	GCNGC	9 cut(s)	445, 495, 715, 961, 1207, 1833, 1906, 2303, 2385
PleI	GAGTC	4 cut(s)	247, 319, 1764, 2176
PpsI	GAGTC	4 cut(s)	247, 319, 1764, 2176
Ppu21I	YACGTR	1 cut(s)	2278
Psp124BI	GAGCTC	1 cut(s)	9
Psp6I	CCWGG	3 cut(s)	762, 913, 1777
PspFI	CCCAGC	3 cut(s)	1695, 1925, 2304
PspGI	CCWGG	3 cut(s)	762, 913, 1777
PspN4I	GGNNCC	5 cut(s)	756, 869, 900, 1047, 1701
PspPI	GGNCC	4 cut(s)	754, 778, 911, 1066
PspXI	VCTCGAGB	1 cut(s)	1318
PsrI	GAACNNNNNNTAC	2 cut(s)	935, 967
PstI	CTGCAG	1 cut(s)	495
PsuI	RGATCY	4 cut(s)	36, 98, 1156, 1350
PvuII	CAGCTG	1 cut(s)	455
RsaI	GTAC	3 cut(s)	305, 1118, 1521
RsaNI	GTAC	3 cut(s)	304, 1117, 1520
RseI	CAYNNNNRTG	1 cut(s)	1965
SacI	GAGCTC	1 cut(s)	9
SaqAI	TTAA	5 cut(s)	204, 467, 573, 1025, 1082
SatI	GCNGC	9 cut(s)	444, 494, 714, 960, 1206, 1832, 1905, 2302, 2384
Sau96I	GGNCC	4 cut(s)	754, 778, 911, 1066
ScaI	AGTACT	1 cut(s)	1118
SchI	GAGTC	4 cut(s)	247, 320, 1764, 2176
ScrFI	CCNGG	3 cut(s)	764, 915, 1779
SduI	GDGCHC	3 cut(s)	9, 651, 2265
SfcI	CTRYAG	4 cut(s)	264, 402, 491, 1588
Sfr274I	CTCGAG	1 cut(s)	1318
SinI	GGWCC	3 cut(s)	754, 911, 1066
SlaI	CTCGAG	1 cut(s)	1318
SmiMI	CAYNNNNRTG	1 cut(s)	1965
SmlI	CTYRAG	2 cut(s)	242, 1318
SmoI	CTYRAG	2 cut(s)	242, 1318
SsiI	CCGC	7 cut(s)	427, 758, 1206, 1418, 1672, 2302, 2383
SspMI	CTAG	7 cut(s)	27, 213, 1161, 1290, 1550, 1865, 2166
SstI	GAGCTC	1 cut(s)	9
StyD4I	CCNGG	3 cut(s)	762, 913, 1777
TaaI	ACNGT	7 cut(s)	403, 746, 872, 947, 1397, 1791, 2267
TaiI	ACGT	2 cut(s)	1200, 2280
TaqI	TCGA	7 cut(s)	87, 396, 803, 1251, 1319, 1980, 2339
TatI	WGTACW	1 cut(s)	1116
TauI	GCSGC	3 cut(s)	1208, 2304, 2386
TfiI	GAWTC	3 cut(s)	562, 592, 1823
Tru1I	TTAA	5 cut(s)	204, 467, 573, 1025, 1082
Tru9I	TTAA	5 cut(s)	204, 467, 573, 1025, 1082
TscAI	CASTG	6 cut(s)	495, 703, 751, 1898, 2265, 2270
TseFI	GTSAC	1 cut(s)	2243
TseI	GCWGC	6 cut(s)	443, 493, 713, 959, 1831, 1904
Tsp45I	GTSAC	1 cut(s)	2243
TspDTI	ATGAA	6 cut(s)	140, 188, 981, 1458, 1527, 1958
TspGWI	ACGGA	2 cut(s)	572, 1350
TspRI	CASTG	6 cut(s)	495, 703, 751, 1898, 2265, 2270
Van91I	CCANNNNNTGG	1 cut(s)	908
VneI	GTGCAC	1 cut(s)	2261
VpaK11BI	GGWCC	3 cut(s)	754, 911, 1066
XapI	RAATTY	4 cut(s)	856, 1220, 1555, 1676
XceI	RCATGY	4 cut(s)	482, 1189, 1672, 1759
XhoI	CTCGAG	1 cut(s)	1318
XmiI	GTMKAC	3 cut(s)	874, 1719, 2370
XmnI	GAANNNNTTC	1 cut(s)	1455
XspI	CTAG	7 cut(s)	27, 213, 1161, 1290, 1550, 1865, 2166
ZrmI	AGTACT	1 cut(s)	1118
Zsp2I	ATGCAT	2 cut(s)	484, 2140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.