Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0014306

Physical Location & Seq

Genomic Coordinates Reverse (-)

14182436 .. 14190499

Gene Structure 8064 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g21160.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGCTTGACCCAATGGACCTGCTCCGATCCAACCTCTCCCGGGTTCGGATCCCGGAACCGACTAACCGCATCTACAAGCAAGAATGCTGCGTCTCTTTCGACACTCCGAGATCGGAAGGTGGTTTGTTCGTTGACATGAACACCTTTCTTGCTTTTGGGAAGGATTACATAGGATGGCACTATGAAAAGACTGGAAACCCAGTTTATCTGCATATAAAGCAAAACAAAGTGTTGGTTCCTGAAGATAGGCCTTTAAAGAAACCAACGCTCATGGCTATAGGTATTGATGGGGGATTTGACAATAATGAACCGGAATATGAAGAATCTCACAGTATTGTTGTGTTGCCTGATTATGTGACTCTACCATTTCCTTCTGTTGAGCTGCCAGAGAAGGTAAGATTGGCGGTTGATAGCATTTTAATGGCTGAGGGTGCAGAGAAGAAAGAACAAATAGCAGCATGGACAGCCGATAAGAAGCAAACGAGTGCATATGCAATGAATTTGCAACAGGTTGACAATGGTACCATCGTTCCTTCTTCTGGGTGGAAATGTTCCAAGTGTGATAAAAGAGACAATCTTTGGATGAATCTAACTGATGGAACGATCCTTTGTGGGAGAAAAAATTGGGATGGTACTGGTGGAAATAACCATGCTGTCGAGTACTATAACGATACAGGATATCCTCTTGCTGTAAAGCTAGGGACAATTACAGCTGATCTAGAAGGAGCAGATGTTTTCTCTTATCCAGAGGATGATAGTGTTGTAGATCCACTTTTAGCACAACATCTGGCATTTTTTGGCATTGACTTCTCCTCATTACAGAAGACGGAGATGACTACTGCTGAAAGAGAGCTTGACCAGAATACCAATTTTGACTGGCACCGTATTCAAGAAAGTGGACAGGAAGTCGAACCGATATTTGGACCTGGTTACACTGGCCTTGTCAATCTTGGAAATAGCTGTTACATGGCAGCAACCATGCAAGTAGTGTTCTCTACGCACTCTTTCTATTCCGGGTACTACAAAAATCAAAGTTTGAAAACAGCATTTGAGAAGGCGCCAGCTGACCCAACTGTAGATCTGAACATGCAGCTAACAAAGTTGGCACATGGTTTGCTATCTGGCAAATACTCTGTTCCTGTATCAGAGGAAAAAGATCAGACAAATGCTATTACCCCAACAACAAATGTGAAACAAGATGGGATACCTCCTCGTATGTTCAAGGCTGTTATAGCTGCCAGTCATCCTGAATTTTCTTCCATGAGACAACAGGATGCCTTGGAGTTCTTCTTGCACTTTCTTGACCAAGTTGAACGGGCTCATGGTGGGAGACCTGAAATTGATCCTGCGAGGAGTTTCAAGTTTGGTATTGAAGATCGTATTATGTGTTCATCTGGAAAAGTTGCGTACAATAGGAGACTGGACTACATTCTTTCCTTGAACATTCCGCTGCATGAAGCTACTAATAAAGAAGAGCTTGCAGCATTTCAGAAACTGAAAGCTGAAAAGGCTTCAGAAGGCAAGGAAGTATCCAGTGATGAAATTGTACGTCCAAGGGTTCCCCTAGAAGCATGCCTTGCAAGCTTTTCGTCACCAGAGGAGATACAGGATTTCTACAGCACTGCCTTGAAGGCCAAGACAACAGCAGTAAAGAGTGCAGGTCTAACTTCATTCCCTGACTATTTGGTGTTGCATATGCGAAAATTCGTAATGGAGGAAGGCTGGGTGCCAAAAAAGCTTGATGTCTATATAGATGTTCCTGATATCATAGATATTAGTCACATGCGCAGTAAGGGACTTCAACATGGAGAGGAGTTGCTACCAGAGAGCGTTCCTGGAGAAGAGGAGGAACCAACTAAGCCTGTGGCGGATGAGAATATTGTTACTCAGCTTGTTTCAATGGGATTTAATAATCTTCACTGTCAGAAAGCTGCCATTAATACCTCAAATGCTGGAGTAGAAGAGGCAATGAATTGGCTACTTTCTCACATGGATGATCCTGATATAGACACACCTATCTCTCAAGGCGGAGGACAAATTGTGGTTGACCAATCAAAAGTGGATATGTTGATTTCATTTGGTTTTCAGGAAGATATTGCTCGGGCTGCATTGAAAGCATCGGGTGGTGACATTGAGAAAGCCACAGATTGGATATTCAACAATCCTGGTGCTTCTGTCTCAACTGATATGGATGCAACAACATCAGAGACAACACCTACTGCTGCTGATTTGGGGCTACCTGATGGAGGAGGAAGATATAGACTTATTGGAATTGTAAGCCATATTGGAACCTCTACACTATGTGGTCACTACGTTGCACACATCTTTAAAGATGGTAGATGGGTAATTTTCAATGATGCGAAGGTTGGGGCTTCCGTCAATCCTCCGAAGGACATGGGTTATCTTTATTTCTTTGAGAGACTAAACAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0016049 GO:0016579 GO:0016787 GO:0019538 GO:0019783 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0036459 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070011 GO:0070646 GO:0070647 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0101005 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA UCH zf-UBP

Protein Analysis

811

Amino Acids

89.8

Weight (kDa)

4.94

Isoelectric Point (pI)

39.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP	PF02148	44 - 118	1.4e-16	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	172 - 665	5.5e-44	Ubiquitin carboxyl-terminal hydrolase
UBA_7	PF22562	484 - 530	9.2e-21	UBA-like domain
UBA	PF00627	484 - 520	1.5e-06	UBA/TS-N domain
UBA	PF00627	542 - 578	1.1e-08	UBA/TS-N domain
UBA_7	PF22562	543 - 582	2.2e-08	UBA-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20630
fragaria_vesca	FvH4_3g21160 FvH4_3g21160
malus_domestica	MD03G1217200.v1.1 MD11G1234900.v1.1
prunus_persica	Prupe.4G192200_v2.0.a1
pyrus_communis	pycom11g20650
rosa_chinensis	RchiOBHm_Chr5g0036241
rosa_laevigata	RLG00000033681
rosa_multiflora	Rmu_sc0000247.1_g000007
rosa_roxburghii	Rroxscaffold_1G00044540
rosa_rugosa	Rorug05G0154300
rosa_samantha	Rh5AG248000 Rh5BG248600 Rh5CG279900 Rh5DG256900
rosa_wichuraiana	Rw5G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1787
Acc36I	ACCTGC	2 cut(s)	27, 1649
Acc65I	GGTACC	1 cut(s)	521
AccB1I	GGYRCC	4 cut(s)	521, 879, 1057, 1726
AccB7I	CCANNNNNTGG	1 cut(s)	2148
AciI	CCGC	5 cut(s)	67, 404, 1448, 1868, 2028
AclWI	GGATC	7 cut(s)	21, 43, 56, 598, 761, 1337, 1991
AcsI	RAATTY	3 cut(s)	499, 1250, 1703
AcuI	CTGAAG	2 cut(s)	261, 1497
AcyI	GRCGYC	1 cut(s)	1058
AdeI	CACNNNGTG	1 cut(s)	2303
AfaI	GTAC	6 cut(s)	523, 634, 662, 1019, 1409, 1548
AfiI	CCNNNNNNNGG	6 cut(s)	40, 45, 539, 920, 2148, 2246
AhdI	GACNNNNNGTC	1 cut(s)	905
AjnI	CCWGG	3 cut(s)	925, 1834, 2164
AjuI	GAANNNNNNNTTGG	4 cut(s)	383, 415, 903, 935
Alw26I	GTCTC	8 cut(s)	97, 564, 1258, 1324, 1411, 2182, 2201, 2413
AlwI	GGATC	7 cut(s)	21, 43, 56, 598, 761, 1337, 1991
AlwNI	CAGNNNCTG	1 cut(s)	1495
Ama87I	CYCGRG	2 cut(s)	39, 2100
AoxI	GGCC	3 cut(s)	248, 937, 1632
ApoI	RAATTY	3 cut(s)	499, 1250, 1703
AseI	ATTAAT	1 cut(s)	1938
Asp718I	GGTACC	1 cut(s)	521
AspLEI	GCGC	2 cut(s)	1060, 1788
AspS9I	GGNCC	2 cut(s)	16, 923
AsuC2I	CCSGG	4 cut(s)	40, 41, 53, 1015
AsuHPI	GGTGA	2 cut(s)	1584, 2138
AvaI	CYCGRG	2 cut(s)	39, 2100
AvaII	GGWCC	2 cut(s)	16, 923
BaeI	ACNNNNGTAYC	4 cut(s)	505, 513, 538, 546
BamHI	GGATCC	1 cut(s)	48
BanI	GGYRCC	4 cut(s)	521, 879, 1057, 1726
BanII	GRGCYC	1 cut(s)	1321
BbsI	GAAGAC	1 cut(s)	830
BbvCI	CCTCAGC	1 cut(s)	426
BccI	CCATC	9 cut(s)	168, 281, 533, 590, 623, 1193, 2237, 2327, 2334
BcgI	CGANNNNNNTGC	2 cut(s)	1569, 1603
BciT130I	CCWGG	3 cut(s)	927, 1836, 2166
BciVI	GTATCC	2 cut(s)	1197, 1540
BcnI	CCSGG	4 cut(s)	40, 41, 53, 1015
BcoDI	GTCTC	8 cut(s)	97, 564, 1258, 1324, 1411, 2182, 2201, 2413
BfaI	CTAG	3 cut(s)	698, 719, 1565
BfmI	CTRYAG	3 cut(s)	276, 1074, 1615
BfoI	RGCGCY	1 cut(s)	1061
BfuAI	ACCTGC	2 cut(s)	27, 1649
BfuI	GTATCC	2 cut(s)	1197, 1540
BglI	GCCNNNNNGGC	1 cut(s)	1631
BglII	AGATCT	1 cut(s)	1078
BmcAI	AGTACT	1 cut(s)	662
Bme1390I	CCNGG	7 cut(s)	40, 41, 53, 927, 1015, 1836, 2166
Bme18I	GGWCC	2 cut(s)	16, 923
BmeRI	GACNNNNNGTC	1 cut(s)	905
BmeT110I	CYCGRG	2 cut(s)	39, 2100
BmgT120I	GGNCC	2 cut(s)	16, 923
BmrFI	CCNGG	7 cut(s)	40, 41, 53, 927, 1015, 1836, 2166
BmrI	ACTGGG	1 cut(s)	194
BmsI	GCATC	5 cut(s)	78, 1264, 2126, 2182, 2347
BmuI	ACTGGG	1 cut(s)	194
BpiI	GAAGAC	1 cut(s)	830
BpmI	CTGGAG	2 cut(s)	1857, 1974
Bpu10I	CCTNAGC	1 cut(s)	426
BpuEI	CTTGAG	1 cut(s)	2007
BpuMI	CCSGG	4 cut(s)	40, 41, 53, 1015
BsaHI	GRCGYC	1 cut(s)	1058
BsaI	GGTCTC	1 cut(s)	1324
BsaJI	CCNNGG	3 cut(s)	39, 1278, 1553
BsaWI	WCCGGW	1 cut(s)	310
BsaXI	ACNNNNNCTCC	2 cut(s)	717, 747
Bsc4I	CCNNNNNNNGG	6 cut(s)	40, 45, 539, 920, 2148, 2246
Bse1I	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1239, 1425, 1533
Bse3DI	GCAATG	2 cut(s)	501, 1974
BseBI	CCWGG	3 cut(s)	927, 1836, 2166
BseDI	CCNNGG	3 cut(s)	39, 1278, 1553
BseGI	GGATG	9 cut(s)	179, 588, 634, 757, 1243, 1279, 1876, 1999, 2197
BseLI	CCNNNNNNNGG	6 cut(s)	40, 45, 539, 920, 2148, 2246
BseMI	GCAATG	2 cut(s)	501, 1974
BseMII	CTCAG	2 cut(s)	417, 1901
BseNI	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1239, 1425, 1533
BseRI	GAGGAG	7 cut(s)	802, 1200, 1366, 1613, 1826, 1859, 2262
BseYI	CCCAGC	1 cut(s)	1722
BsgI	GTGCAG	2 cut(s)	453, 1677
BshFI	GGCC	3 cut(s)	250, 939, 1634
BshNI	GGYRCC	4 cut(s)	521, 879, 1057, 1726
BsiHKCI	CYCGRG	2 cut(s)	39, 2100
BsiSI	CCGG	4 cut(s)	40, 53, 311, 1014
BslFI	GGGAC	2 cut(s)	715, 1809
BslI	CCNNNNNNNGG	6 cut(s)	40, 45, 539, 920, 2148, 2246
BsmAI	GTCTC	8 cut(s)	97, 564, 1258, 1324, 1411, 2182, 2201, 2413
BsmBI	CGTCTC	1 cut(s)	97
BsmFI	GGGAC	2 cut(s)	715, 1809
BsmI	GAATGC	1 cut(s)	89
BsnI	GGCC	3 cut(s)	250, 939, 1634
Bso31I	GGTCTC	1 cut(s)	1324
BsoBI	CYCGRG	2 cut(s)	39, 2100
Bsp1286I	GDGCHC	1 cut(s)	1321
BspACI	CCGC	5 cut(s)	67, 404, 1448, 1868, 2028
BspANI	GGCC	3 cut(s)	250, 939, 1634
BspCNI	CTCAG	2 cut(s)	418, 1900
BspMI	ACCTGC	2 cut(s)	27, 1649
BspPI	GGATC	7 cut(s)	21, 43, 56, 598, 761, 1337, 1991
BspQI	GCTCTTC	1 cut(s)	1467
BspT107I	GGYRCC	4 cut(s)	521, 879, 1057, 1726
BspTNI	GGTCTC	1 cut(s)	1324
BsrDI	GCAATG	2 cut(s)	501, 1974
BsrI	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1239, 1425, 1533
BssECI	CCNNGG	3 cut(s)	39, 1278, 1553
BssNI	GRCGYC	1 cut(s)	1058
BssT1I	CCWWGG	2 cut(s)	1278, 1553
Bst2UI	CCWGG	3 cut(s)	927, 1836, 2166
Bst4CI	ACNGT	5 cut(s)	332, 884, 1075, 1922, 2429
Bst6I	CTCTTC	3 cut(s)	1467, 1836, 1956
BstACI	GRCGYC	1 cut(s)	1058
BstAPI	GCANNNNNTGC	1 cut(s)	1577
BstC8I	GCNNGC	4 cut(s)	1062, 1479, 1573, 1582
BstDEI	CTNAG	3 cut(s)	426, 1857, 1887
BstENI	CCTNNNNNAGG	1 cut(s)	2244
BstF5I	GGATG	9 cut(s)	179, 588, 634, 757, 1243, 1279, 1876, 1999, 2197
BstH2I	RGCGCY	1 cut(s)	1061
BstHHI	GCGC	2 cut(s)	1060, 1788
BstMAI	GTCTC	8 cut(s)	97, 564, 1258, 1324, 1411, 2182, 2201, 2413
BstNI	CCWGG	3 cut(s)	927, 1836, 2166
BstNSI	RCATGY	3 cut(s)	1090, 1575, 1786
BstSCI	CCNGG	7 cut(s)	38, 39, 51, 925, 1013, 1834, 2164
BstSFI	CTRYAG	3 cut(s)	276, 1074, 1615
BstV2I	GAAGAC	1 cut(s)	830
BstX2I	RGATCY	3 cut(s)	48, 766, 1078
BstYI	RGATCY	3 cut(s)	48, 766, 1078
BsuI	GTATCC	2 cut(s)	1197, 1540
BsuRI	GGCC	3 cut(s)	250, 939, 1634
BtsCI	GGATG	9 cut(s)	179, 588, 634, 757, 1243, 1279, 1876, 1999, 2197
BtsI	GCAGTG	1 cut(s)	1620
BtsIMutI	CAGTG	4 cut(s)	933, 1540, 1620, 1918
BveI	ACCTGC	2 cut(s)	27, 1649
Cac8I	GCNNGC	4 cut(s)	1062, 1479, 1573, 1582
CaiI	CAGNNNCTG	1 cut(s)	1495
CfoI	GCGC	2 cut(s)	1060, 1788
Cfr13I	GGNCC	2 cut(s)	16, 923
Cfr9I	CCCGGG	1 cut(s)	39
CseI	GACGC	1 cut(s)	79
CsiI	ACCWGGT	1 cut(s)	925
Csp6I	GTAC	6 cut(s)	522, 633, 661, 1018, 1408, 1547
CviQI	GTAC	6 cut(s)	522, 633, 661, 1018, 1408, 1547
DdeI	CTNAG	3 cut(s)	426, 1857, 1887
DinI	GGCGCC	1 cut(s)	1059
DraI	TTTAAA	2 cut(s)	255, 2329
DraIII	CACNNNGTG	1 cut(s)	2303
DriI	GACNNNNNGTC	1 cut(s)	905
Eam1104I	CTCTTC	3 cut(s)	1467, 1836, 1956
Eam1105I	GACNNNNNGTC	1 cut(s)	905
EarI	CTCTTC	3 cut(s)	1467, 1836, 1956
EciI	GGCGGA	2 cut(s)	1883, 2043
Eco130I	CCWWGG	2 cut(s)	1278, 1553
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	1 cut(s)	1321
Eco31I	GGTCTC	1 cut(s)	1324
Eco32I	GATATC	2 cut(s)	680, 1765
Eco47I	GGWCC	2 cut(s)	16, 923
Eco57I	CTGAAG	2 cut(s)	261, 1497
Eco88I	CYCGRG	2 cut(s)	39, 2100
EcoNI	CCTNNNNNAGG	1 cut(s)	2244
EcoRII	CCWGG	3 cut(s)	925, 1834, 2164
EcoRV	GATATC	2 cut(s)	680, 1765
EcoT14I	CCWWGG	2 cut(s)	1278, 1553
EcoT38I	GRGCYC	1 cut(s)	1321
EgeI	GGCGCC	1 cut(s)	1059
EheI	GGCGCC	1 cut(s)	1059
ErhI	CCWWGG	2 cut(s)	1278, 1553
Esp3I	CGTCTC	1 cut(s)	97
FalI	AAGNNNNNCTT	4 cut(s)	1461, 1493, 1560, 1592
FaqI	GGGAC	2 cut(s)	715, 1809
FauNDI	CATATG	2 cut(s)	490, 1695
FokI	GGATG	9 cut(s)	186, 595, 641, 764, 1230, 1286, 1883, 2006, 2204
FriOI	GRGCYC	1 cut(s)	1321
FspBI	CTAG	3 cut(s)	698, 719, 1565
FspI	TGCGCA	1 cut(s)	1787
GlaI	GCGC	2 cut(s)	1059, 1787
GsaI	CCCAGC	1 cut(s)	1726
GsuI	CTGGAG	2 cut(s)	1857, 1974
HaeII	RGCGCY	1 cut(s)	1061
HaeIII	GGCC	3 cut(s)	250, 939, 1634
HapII	CCGG	4 cut(s)	40, 53, 311, 1014
HgaI	GACGC	1 cut(s)	79
HhaI	GCGC	2 cut(s)	1060, 1788
Hin1I	GRCGYC	1 cut(s)	1058
Hin6I	GCGC	2 cut(s)	1058, 1786
HinP1I	GCGC	2 cut(s)	1058, 1786
HincII	GTYRAC	3 cut(s)	133, 514, 2047
HindII	GTYRAC	3 cut(s)	133, 514, 2047
HindIII	AAGCTT	2 cut(s)	1582, 1736
HinfI	GANTC	3 cut(s)	323, 358, 586
HpaII	CCGG	4 cut(s)	40, 53, 311, 1014
HphI	GGTGA	2 cut(s)	1584, 2138
Hpy166II	GTNNAC	4 cut(s)	133, 514, 899, 2047
Hpy8I	GTNNAC	4 cut(s)	133, 514, 899, 2047
HpyCH4III	ACNGT	5 cut(s)	332, 884, 1075, 1922, 2429
HpyCH4IV	ACGT	2 cut(s)	1549, 2313
HpyF3I	CTNAG	3 cut(s)	426, 1857, 1887
HpySE526I	ACGT	2 cut(s)	1549, 2313
Hsp92I	GRCGYC	1 cut(s)	1058
HspAI	GCGC	2 cut(s)	1058, 1786
KasI	GGCGCC	1 cut(s)	1057
KpnI	GGTACC	1 cut(s)	525
LguI	GCTCTTC	1 cut(s)	1467
LmnI	GCTCC	2 cut(s)	27, 725
LweI	GCATC	5 cut(s)	78, 1264, 2126, 2182, 2347
MabI	ACCWGGT	1 cut(s)	925
MaeI	CTAG	3 cut(s)	698, 719, 1565
MaeII	ACGT	2 cut(s)	1549, 2313
MaeIII	GTNAC	8 cut(s)	355, 929, 962, 1590, 1778, 1882, 2126, 2306
MflI	RGATCY	3 cut(s)	48, 766, 1078
MhlI	GDGCHC	1 cut(s)	1321
Mly113I	GGCGCC	1 cut(s)	1058
MlyI	GAGTC	1 cut(s)	352
MmeI	TCCRAC	1 cut(s)	54
MseI	TTAA	5 cut(s)	254, 419, 1908, 1938, 2328
MslI	CAYNNNNRTG	2 cut(s)	419, 1992
MspA1I	CMGCKG	3 cut(s)	713, 1064, 1450
MspI	CCGG	4 cut(s)	40, 53, 311, 1014
MspR9I	CCNGG	7 cut(s)	40, 41, 53, 927, 1015, 1836, 2166
Mva1269I	GAATGC	1 cut(s)	89
MvaI	CCWGG	3 cut(s)	927, 1836, 2166
NarI	GGCGCC	1 cut(s)	1058
NciI	CCSGG	4 cut(s)	40, 41, 53, 1015
NdeI	CATATG	2 cut(s)	490, 1695
NmuCI	GTSAC	5 cut(s)	355, 1590, 1778, 2126, 2306
NsbI	TGCGCA	1 cut(s)	1787
NspI	RCATGY	3 cut(s)	1090, 1575, 1786
PaeI	GCATGC	1 cut(s)	1575
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	1 cut(s)	1467
PctI	GAATGC	1 cut(s)	89
PfeI	GAWTC	2 cut(s)	323, 586
PflMI	CCANNNNNTGG	1 cut(s)	2148
PfoI	TCCNGGA	2 cut(s)	51, 1834
PleI	GAGTC	1 cut(s)	352
PluTI	GGCGCC	1 cut(s)	1061
PpsI	GAGTC	1 cut(s)	352
PshBI	ATTAAT	1 cut(s)	1938
Psp6I	CCWGG	3 cut(s)	925, 1834, 2164
PspFI	CCCAGC	1 cut(s)	1722
PspGI	CCWGG	3 cut(s)	925, 1834, 2164
PspPI	GGNCC	2 cut(s)	16, 923
PsrI	GAACNNNNNNTAC	2 cut(s)	1372, 1404
PstNI	CAGNNNCTG	1 cut(s)	1495
PsuI	RGATCY	3 cut(s)	48, 766, 1078
PvuII	CAGCTG	2 cut(s)	713, 1064
RsaI	GTAC	6 cut(s)	523, 634, 662, 1019, 1409, 1548
RsaNI	GTAC	6 cut(s)	522, 633, 661, 1018, 1408, 1547
RseI	CAYNNNNRTG	2 cut(s)	419, 1992
SapI	GCTCTTC	1 cut(s)	1467
SaqAI	TTAA	5 cut(s)	254, 419, 1908, 1938, 2328
Sau96I	GGNCC	2 cut(s)	16, 923
ScaI	AGTACT	1 cut(s)	662
SchI	GAGTC	1 cut(s)	352
ScrFI	CCNGG	7 cut(s)	40, 41, 53, 927, 1015, 1836, 2166
SduI	GDGCHC	1 cut(s)	1321
SexAI	ACCWGGT	1 cut(s)	925
SfaNI	GCATC	5 cut(s)	78, 1264, 2126, 2182, 2347
SfcI	CTRYAG	3 cut(s)	276, 1074, 1615
SfoI	GGCGCC	1 cut(s)	1059
SinI	GGWCC	2 cut(s)	16, 923
SmaI	CCCGGG	1 cut(s)	41
SmiMI	CAYNNNNRTG	2 cut(s)	419, 1992
SmlI	CTYRAG	1 cut(s)	2022
SmoI	CTYRAG	1 cut(s)	2022
SphI	GCATGC	1 cut(s)	1575
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	5 cut(s)	67, 404, 1448, 1868, 2028
SspDI	GGCGCC	1 cut(s)	1057
SspI	AATATT	1 cut(s)	1879
SspMI	CTAG	3 cut(s)	698, 719, 1565
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	7 cut(s)	38, 39, 51, 925, 1013, 1834, 2164
StyI	CCWWGG	2 cut(s)	1278, 1553
TaaI	ACNGT	5 cut(s)	332, 884, 1075, 1922, 2429
TaiI	ACGT	2 cut(s)	1552, 2316
TaqI	TCGA	3 cut(s)	99, 657, 909
TatI	WGTACW	1 cut(s)	660
TfiI	GAWTC	2 cut(s)	323, 586
Tru1I	TTAA	5 cut(s)	254, 419, 1908, 1938, 2328
Tru9I	TTAA	5 cut(s)	254, 419, 1908, 1938, 2328
TscAI	CASTG	4 cut(s)	940, 1540, 1627, 1925
TseFI	GTSAC	5 cut(s)	355, 1590, 1778, 2126, 2306
Tsp45I	GTSAC	5 cut(s)	355, 1590, 1778, 2126, 2306
TspGWI	ACGGA	2 cut(s)	842, 2365
TspMI	CCCGGG	1 cut(s)	39
TspRI	CASTG	4 cut(s)	940, 1540, 1627, 1925
Van91I	CCANNNNNTGG	1 cut(s)	2148
VpaK11BI	GGWCC	2 cut(s)	16, 923
VspI	ATTAAT	1 cut(s)	1938
XagI	CCTNNNNNAGG	1 cut(s)	2244
XapI	RAATTY	3 cut(s)	499, 1250, 1703
XbaI	TCTAGA	1 cut(s)	718
XceI	RCATGY	3 cut(s)	1090, 1575, 1786
XmaI	CCCGGG	1 cut(s)	39
XspI	CTAG	3 cut(s)	698, 719, 1565
ZrmI	AGTACT	1 cut(s)	662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.