Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014306

Physical Location & Seq

Genomic Coordinates Forward (+)

30140378 .. 30149102

Gene Structure 8725 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1217200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGCTGGACCCCATGGACCTGCTCCGATCCAATCTCTCCCGGGTTCGGATCCCGGAACCCACCAACCGCATCTACAAGCAAGAATGCTGCATCTCTTTCGACACTCCGAGATCAGAAGGTGGTTTGTTTGTGGACATGAACACATTTCTTGCTTTTGGGAAGGATTGCGTGGCCTGGAACTATGAAAAGACTGGAAATCCAGTTTATTTGCATATAAAGCAAACAAAAGTGTTGGTTCCTGAAGATAGGCCTTTGAAGAAACCCACACTCGTGGCTATAGGTGTCGATGGGGGATTTGATAACAATGAACCTGAATATGAAGAAACACACAATATAGTCCTACTGCCTGATTATGTAACTCTTCCATTTCCTTCTGTCGAATTACCAGAGAAGGTAAGATTGGCTGTTGATGCCATTTTATTGGCTGAGGGTGCTGAGAGGAAAGAACAACTTGAAGCATGGACGGCTGAGAAGAAGCAAGTTAGTGCTTATGCAACGAATTTGCAACAGATTGACAATGGTATTATTATTCCTTCATCTGGCTGGAAATGTTGCAAGTGTGATAAAAGAGAAAACCTTTGGTTAAATCTAACTGATGGAATGATCCTTTGTGGGAGAAAAAATTGGGATGGAACTGGTGGCAACAACCATGCCATTGAACACTATAAGGAAACGGGCTATCCTCTTGCTGTAAAGCTGGGGACAATTACAGCTGATCTGGAAGGAGCAGATGTTTTCTCATACCCAGAGGATGATAGTGTATTAGACCCACTTTTGGCGCAACATCTGGCATTTTTTGGCATTGATTTTTCATCATTACTGAAGACAGAAATGACAACTGCTGAAAGAGAGCTTGACCAGAATACCAATTTTGACTGGAACCGGATTCAAGAAAGTGGACAGGAGGTTGAACTGATATTTGGACCTGGTTACACTGGACTTGTCAATCTTGGAAACAGCTGTTATATGGCAGCGACTATGCAAGTTGTGTTTTCAACACACTCTTTCTATTCCGGATACTTCAAGAATCAAAGTTTGAAAATGGCTTTTGAGAAAGCACCAGCTGACCCCACTGTAGACCTGAACATGCAGTTAACAAAGCTGGCGCATGGTTTGCTGTCTGGTAAATACTCTGTACCTGTAACCGAGGAAAAAGATCATACAAATGCCATTGCCCCTACAACAAAATTAAAACAGGAAGGGATACCTCCTCGTATGTTTAAAGCGCTTATAGCTGCCAGCCATCCTGAATTTTCCTCCATGAGACAACAGGATGCCTTGGAGTTCTTCCTTCATTTTCTTGACCAAGTTGAACGAGTTCATTCTGGAAGACCTGAAGTGGATCCTGCGAGGAGTTTTAAGTTTGGCATTGAAGATCGTATTATGTGTTCGTCTGGGAAAGTTGCTTACAATAGAAGGCTTGACTACATTCTCTCATTAAACATCCCACTTCATGCAGCCACTAATAAAGAAGAACTTGAAGCCTTTCAAAAATTGAAAGCAGAAAAGATTGCGGAAGGCAAGGAAGTATCCAGTGATGAGATTGTACGTCCAAGGGTTCCTCTAGAAGCATGCCTTGCAAGCTTTTCATCTCCCGAAGAGATACAGGATTTTTACAGCACTGGTTTGAAGGCCAAGACAACGGCTGTGAAGAGTGCAGGTCTAACTTCGTTCCCTGATTATCTGGTGTTGCACATGCGAAAGTTTGTAATGGAAGTGGGCTGGGTGCCCAAAAAACTAGATGTATATATAGATGTTCCTGATATCATAGATATCAGTCACATGCGCAGCAAGGGACTCCAACCTGGTGAAGAGTTGTTGCCGGAGGGCGGGGAAGAGGAGGAACCAAATGAGCCTGTTGCCGATGAGAGTATTGTTTTCCAGCTTGTTTCAATGGGTTTTAATCAGTTACACTGTCAGAAAGCTGCCATTAATACGTCAAATACTGGAGTGGAAGAGGCAATGAATTGGCTACTTTCACACATGGATGATGCAGATATAGATGCTCCTATTTCTCAAGGGGGACAGTGTGCGGTTGATCAATCAAAAGTTGATATGTTGATTTCATTTGGTTTTCAAGAAGACACTGCTCGGAAGGCACTGAAAGCATCGGGTGGTGACATCGAGAAAGCCACAGATTGGATATTCAACAATCCTGATGCTTCTGCTTCATCGGACATGGATGCGACAACATCTGACACTGCACCTACTGCGATTGATGCTGGACTGCCTGATGGAGCAGGGAGATATAGACTCATTGGAATTGTAAGCCACATCGGAACATCTACTCAATGTGGTCATTATGTTGCTCACATCTTAAAAGATGGCAGATGGGTTATTTTCAATGACGACAAAGTTGGAGCTTCCGTTAATCCTCCCAAGGACATGGGATACCTTTACTTCTTTGAGAGACTTAGTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004843 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010154 GO:0016049 GO:0016579 GO:0016787 GO:0019538 GO:0019783 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0036459 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0060560 GO:0061458 GO:0070011 GO:0070646 GO:0070647 GO:0071695 GO:0071704 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0101005 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UBA UCH zf-UBP

Protein Analysis

808

Amino Acids

89.62

Weight (kDa)

4.91

Isoelectric Point (pI)

37.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UBP_var	PF17807	15 - 76	4.7e-22	Variant UBP zinc finger
zf-UBP	PF02148	184 - 257	1.1e-17	Zn-finger in ubiquitin-hydrolases and other protein
UCH	PF00443	312 - 802	5.1e-45	Ubiquitin carboxyl-terminal hydrolase
UBA_7	PF22562	622 - 669	1.8e-19	UBA-like domain
UBA	PF00627	622 - 658	6e-06	UBA/TS-N domain
UBA	PF00627	679 - 715	2.6e-09	UBA/TS-N domain
UBA_7	PF22562	680 - 722	6e-11	UBA-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20630
fragaria_vesca	FvH4_3g21160 FvH4_3g21160
malus_domestica	MD03G1217200.v1.1 MD11G1234900.v1.1
prunus_persica	Prupe.4G192200_v2.0.a1
pyrus_communis	pycom11g20650
rosa_chinensis	RchiOBHm_Chr5g0036241
rosa_laevigata	RLG00000033681
rosa_multiflora	Rmu_sc0000247.1_g000007
rosa_roxburghii	Rroxscaffold_1G00044540
rosa_rugosa	Rorug05G0154300
rosa_samantha	Rh5AG248000 Rh5BG248600 Rh5CG279900 Rh5DG256900
rosa_wichuraiana	Rw5G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1787
Acc36I	ACCTGC	2 cut(s)	27, 1649
AccB1I	GGYRCC	1 cut(s)	1726
AccB7I	CCANNNNNTGG	1 cut(s)	2139
AccI	GTMKAC	1 cut(s)	1077
AccIII	TCCGGA	1 cut(s)	1013
AciI	CCGC	4 cut(s)	67, 1514, 1830, 2033
AclWI	GGATC	6 cut(s)	21, 43, 56, 598, 1337, 1350
AcsI	RAATTY	2 cut(s)	499, 1250
AcuI	CTGAAG	3 cut(s)	261, 842, 1356
AfaI	GTAC	2 cut(s)	1137, 1548
AfeI	AGCGCT	1 cut(s)	1227
AfiI	CCNNNNNNNGG	5 cut(s)	45, 539, 775, 1829, 2139
AjnI	CCWGG	3 cut(s)	173, 925, 1804
AjuI	GAANNNNNNNTTGG	4 cut(s)	383, 415, 903, 935
AleI	CACNNNNGTG	1 cut(s)	269
Alw26I	GTCTC	2 cut(s)	1258, 2404
AlwI	GGATC	6 cut(s)	21, 43, 56, 598, 1337, 1350
Ama87I	CYCGRG	1 cut(s)	39
Aor13HI	TCCGGA	1 cut(s)	1013
Aor51HI	AGCGCT	1 cut(s)	1227
AoxI	GGCC	3 cut(s)	171, 248, 1632
ApeKI	GCWGC	6 cut(s)	87, 971, 1235, 1457, 1788, 1925
ApoI	RAATTY	2 cut(s)	499, 1250
AseI	ATTAAT	1 cut(s)	1932
Asp700I	GAANNNNTTC	2 cut(s)	1317, 1485
AspLEI	GCGC	4 cut(s)	781, 1108, 1228, 1788
AspS9I	GGNCC	3 cut(s)	7, 16, 923
AsuC2I	CCSGG	3 cut(s)	40, 41, 53
AsuHPI	GGTGA	2 cut(s)	1820, 2129
AvaI	CYCGRG	1 cut(s)	39
AvaII	GGWCC	3 cut(s)	7, 16, 923
BaeGI	GKGCMC	1 cut(s)	1731
BamHI	GGATCC	2 cut(s)	48, 1342
BanI	GGYRCC	1 cut(s)	1726
BauI	CACGAG	1 cut(s)	269
BbsI	GAAGAC	3 cut(s)	830, 1336, 2088
BbvCI	CCTCAGC	1 cut(s)	426
BbvI	GCAGC	6 cut(s)	74, 983, 1222, 1469, 1800, 1912
BccI	CCATC	7 cut(s)	281, 590, 623, 1251, 2228, 2318, 2325
BceAI	ACGGC	2 cut(s)	480, 1659
BcgI	CGANNNNNNTGC	2 cut(s)	79, 113
BciT130I	CCWGG	3 cut(s)	175, 927, 1806
BciVI	GTATCC	4 cut(s)	1010, 1197, 1540, 2384
BclI	TGATCA	1 cut(s)	2038
BcnI	CCSGG	3 cut(s)	40, 41, 53
BcoDI	GTCTC	2 cut(s)	1258, 2404
BfaI	CTAG	2 cut(s)	1565, 1739
BfmI	CTRYAG	2 cut(s)	276, 1074
BfoI	RGCGCY	1 cut(s)	1229
BfuAI	ACCTGC	2 cut(s)	27, 1649
BfuI	GTATCC	4 cut(s)	1010, 1197, 1540, 2384
BisI	GCNGC	6 cut(s)	88, 972, 1236, 1458, 1789, 1926
BlsI	GCNGC	6 cut(s)	89, 973, 1237, 1459, 1790, 1927
Bme1390I	CCNGG	6 cut(s)	40, 41, 53, 175, 927, 1806
Bme18I	GGWCC	3 cut(s)	7, 16, 923
BmeT110I	CYCGRG	1 cut(s)	39
BmgT120I	GGNCC	3 cut(s)	7, 16, 923
BmiI	GGNNCC	9 cut(s)	9, 50, 57, 237, 881, 1344, 1560, 1728, 1845
BmrFI	CCNGG	6 cut(s)	40, 41, 53, 175, 927, 1806
BpiI	GAAGAC	3 cut(s)	830, 1336, 2088
BpmI	CTGGAG	1 cut(s)	1968
Bpu10I	CCTNAGC	1 cut(s)	426
BpuEI	CTTGAG	1 cut(s)	2001
BpuMI	CCSGG	3 cut(s)	40, 41, 53
BsaJI	CCNNGG	6 cut(s)	12, 39, 1146, 1278, 1553, 2379
BsaWI	WCCGGW	2 cut(s)	882, 1013
BsaXI	ACNNNNNCTCC	4 cut(s)	717, 747, 2352, 2382
Bsc4I	CCNNNNNNNGG	5 cut(s)	45, 539, 775, 1829, 2139
Bse1I	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1533, 1627, 1951
Bse3DI	GCAATG	2 cut(s)	1170, 1968
BseAI	TCCGGA	1 cut(s)	1013
BseBI	CCWGG	3 cut(s)	175, 927, 1806
BseDI	CCNNGG	6 cut(s)	12, 39, 1146, 1278, 1553, 2379
BseGI	GGATG	7 cut(s)	634, 757, 1243, 1279, 1443, 1993, 2188
BseLI	CCNNNNNNNGG	5 cut(s)	45, 539, 775, 1829, 2139
BseMI	GCAATG	2 cut(s)	1170, 1968
BseMII	CTCAG	3 cut(s)	417, 426, 459
BseNI	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1533, 1627, 1951
BseRI	GAGGAG	3 cut(s)	1200, 1366, 1853
BseSI	GKGCMC	1 cut(s)	1731
BseXI	GCAGC	6 cut(s)	74, 983, 1222, 1469, 1800, 1912
BseYI	CCCAGC	2 cut(s)	697, 1722
BsgI	GTGCAG	2 cut(s)	1677, 2187
BshFI	GGCC	3 cut(s)	173, 250, 1634
BshNI	GGYRCC	1 cut(s)	1726
BsiHKCI	CYCGRG	1 cut(s)	39
BsiSI	CCGG	5 cut(s)	40, 53, 883, 1014, 1823
BslFI	GGGAC	3 cut(s)	715, 1809, 2037
BslI	CCNNNNNNNGG	5 cut(s)	45, 539, 775, 1829, 2139
BsmAI	GTCTC	2 cut(s)	1258, 2404
BsmFI	GGGAC	3 cut(s)	715, 1809, 2037
BsmI	GAATGC	1 cut(s)	89
BsnI	GGCC	3 cut(s)	173, 250, 1634
BsoBI	CYCGRG	1 cut(s)	39
Bsp1286I	GDGCHC	1 cut(s)	1731
Bsp13I	TCCGGA	1 cut(s)	1013
Bsp143I	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
Bsp19I	CCATGG	1 cut(s)	12
BspACI	CCGC	4 cut(s)	67, 1514, 1830, 2033
BspANI	GGCC	3 cut(s)	173, 250, 1634
BspCNI	CTCAG	3 cut(s)	418, 427, 460
BspEI	TCCGGA	1 cut(s)	1013
BspLI	GGNNCC	9 cut(s)	9, 50, 57, 237, 881, 1344, 1560, 1728, 1845
BspMI	ACCTGC	2 cut(s)	27, 1649
BspPI	GGATC	6 cut(s)	21, 43, 56, 598, 1337, 1350
BspT107I	GGYRCC	1 cut(s)	1726
BsrDI	GCAATG	2 cut(s)	1170, 1968
BsrI	ACTGG	8 cut(s)	196, 200, 640, 881, 940, 1533, 1627, 1951
BssECI	CCNNGG	6 cut(s)	12, 39, 1146, 1278, 1553, 2379
BssMI	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
BssSI	CACGAG	1 cut(s)	269
BssT1I	CCWWGG	4 cut(s)	12, 1278, 1553, 2379
Bst2BI	CACGAG	1 cut(s)	269
Bst2UI	CCWGG	3 cut(s)	175, 927, 1806
Bst4CI	ACNGT	3 cut(s)	1075, 1916, 2028
Bst6I	CTCTTC	6 cut(s)	366, 1593, 1646, 1806, 1830, 1950
BstAPI	GCANNNNNTGC	3 cut(s)	1114, 1577, 2210
BstC8I	GCNNGC	4 cut(s)	1104, 1240, 1573, 1582
BstDEI	CTNAG	4 cut(s)	426, 435, 468, 2414
BstDSI	CCRYGG	1 cut(s)	12
BstF5I	GGATG	7 cut(s)	634, 757, 1243, 1279, 1443, 1993, 2188
BstH2I	RGCGCY	1 cut(s)	1229
BstHHI	GCGC	4 cut(s)	781, 1108, 1228, 1788
BstKTI	GATC	9 cut(s)	29, 51, 113, 606, 718, 1159, 1345, 1378, 2041
BstMAI	GTCTC	2 cut(s)	1258, 2404
BstMBI	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
BstNI	CCWGG	3 cut(s)	175, 927, 1806
BstNSI	RCATGY	4 cut(s)	1090, 1575, 1699, 1786
BstSCI	CCNGG	6 cut(s)	38, 39, 51, 173, 925, 1804
BstSFI	CTRYAG	2 cut(s)	276, 1074
BstSLI	GKGCMC	1 cut(s)	1731
BstV1I	GCAGC	6 cut(s)	74, 983, 1222, 1469, 1800, 1912
BstV2I	GAAGAC	3 cut(s)	830, 1336, 2088
BstX2I	RGATCY	2 cut(s)	48, 1342
BstXI	CCANNNNNNTGG	3 cut(s)	271, 421, 2386
BstYI	RGATCY	2 cut(s)	48, 1342
BsuI	GTATCC	4 cut(s)	1010, 1197, 1540, 2384
BsuRI	GGCC	3 cut(s)	173, 250, 1634
BtgI	CCRYGG	1 cut(s)	12
BtsCI	GGATG	7 cut(s)	634, 757, 1243, 1279, 1443, 1993, 2188
BtsI	GCAGTG	2 cut(s)	2085, 2199
BtsIMutI	CAGTG	9 cut(s)	933, 1071, 1540, 1620, 1912, 2033, 2085, 2099, 2199
BveI	ACCTGC	2 cut(s)	27, 1649
Cac8I	GCNNGC	4 cut(s)	1104, 1240, 1573, 1582
CfoI	GCGC	4 cut(s)	781, 1108, 1228, 1788
Cfr13I	GGNCC	3 cut(s)	7, 16, 923
Cfr9I	CCCGGG	1 cut(s)	39
CsiI	ACCWGGT	2 cut(s)	925, 1804
Csp6I	GTAC	2 cut(s)	1136, 1547
CviQI	GTAC	2 cut(s)	1136, 1547
DdeI	CTNAG	4 cut(s)	426, 435, 468, 2414
DpnI	GATC	9 cut(s)	28, 50, 112, 605, 717, 1158, 1344, 1377, 2040
DpnII	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
DraI	TTTAAA	1 cut(s)	1222
Eam1104I	CTCTTC	6 cut(s)	366, 1593, 1646, 1806, 1830, 1950
EarI	CTCTTC	6 cut(s)	366, 1593, 1646, 1806, 1830, 1950
Eco130I	CCWWGG	4 cut(s)	12, 1278, 1553, 2379
Eco147I	AGGCCT	1 cut(s)	250
Eco32I	GATATC	2 cut(s)	1765, 1774
Eco47I	GGWCC	3 cut(s)	7, 16, 923
Eco47III	AGCGCT	1 cut(s)	1227
Eco57I	CTGAAG	3 cut(s)	261, 842, 1356
Eco88I	CYCGRG	1 cut(s)	39
EcoRII	CCWGG	3 cut(s)	173, 925, 1804
EcoRV	GATATC	2 cut(s)	1765, 1774
EcoT14I	CCWWGG	4 cut(s)	12, 1278, 1553, 2379
ErhI	CCWWGG	4 cut(s)	12, 1278, 1553, 2379
FalI	AAGNNNNNCTT	6 cut(s)	435, 467, 1461, 1493, 1560, 1592
FaqI	GGGAC	3 cut(s)	715, 1809, 2037
FauI	CCCGC	1 cut(s)	1823
FbaI	TGATCA	1 cut(s)	2038
FblI	GTMKAC	1 cut(s)	1077
Fnu4HI	GCNGC	6 cut(s)	88, 972, 1236, 1458, 1789, 1926
FokI	GGATG	7 cut(s)	641, 764, 1230, 1286, 1430, 2000, 2195
Fsp4HI	GCNGC	6 cut(s)	88, 972, 1236, 1458, 1789, 1926
FspBI	CTAG	2 cut(s)	1565, 1739
FspI	TGCGCA	1 cut(s)	1787
GlaI	GCGC	4 cut(s)	780, 1107, 1227, 1787
GluI	GCNGC	6 cut(s)	88, 972, 1236, 1458, 1789, 1926
GsaI	CCCAGC	2 cut(s)	701, 1726
GsuI	CTGGAG	1 cut(s)	1968
HaeII	RGCGCY	1 cut(s)	1229
HaeIII	GGCC	3 cut(s)	173, 250, 1634
HapII	CCGG	5 cut(s)	40, 53, 883, 1014, 1823
HhaI	GCGC	4 cut(s)	781, 1108, 1228, 1788
Hin6I	GCGC	4 cut(s)	779, 1106, 1226, 1786
HinP1I	GCGC	4 cut(s)	779, 1106, 1226, 1786
HincII	GTYRAC	1 cut(s)	1095
HindII	GTYRAC	1 cut(s)	1095
HindIII	AAGCTT	1 cut(s)	1582
HinfI	GANTC	4 cut(s)	886, 1027, 1797, 2253
HpaI	GTTAAC	1 cut(s)	1095
HpaII	CCGG	5 cut(s)	40, 53, 883, 1014, 1823
HphI	GGTGA	2 cut(s)	1820, 2129
Hpy166II	GTNNAC	4 cut(s)	133, 899, 1078, 1095
Hpy188I	TCNGA	9 cut(s)	26, 48, 108, 115, 1920, 2094, 2176, 2197, 2279
Hpy8I	GTNNAC	4 cut(s)	133, 899, 1078, 1095
HpyCH4III	ACNGT	3 cut(s)	1075, 1916, 2028
HpyCH4IV	ACGT	2 cut(s)	1549, 1937
HpyF3I	CTNAG	4 cut(s)	426, 435, 468, 2414
HpySE526I	ACGT	2 cut(s)	1549, 1937
HspAI	GCGC	4 cut(s)	779, 1106, 1226, 1786
Kpn2I	TCCGGA	1 cut(s)	1013
Ksp22I	TGATCA	1 cut(s)	2038
KspAI	GTTAAC	1 cut(s)	1095
Kzo9I	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
LmnI	GCTCC	5 cut(s)	27, 725, 2011, 2237, 2360
Lsp1109I	GCAGC	6 cut(s)	74, 983, 1222, 1469, 1800, 1912
MabI	ACCWGGT	2 cut(s)	925, 1804
MaeI	CTAG	2 cut(s)	1565, 1739
MaeII	ACGT	2 cut(s)	1549, 1937
MaeIII	GTNAC	6 cut(s)	355, 929, 1141, 1778, 1908, 2117
MalI	GATC	9 cut(s)	28, 50, 112, 605, 717, 1158, 1344, 1377, 2040
MboI	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
MflI	RGATCY	2 cut(s)	48, 1342
MhlI	GDGCHC	1 cut(s)	1731
MlyI	GAGTC	2 cut(s)	1791, 2247
MmeI	TCCRAC	2 cut(s)	1825, 2338
MroI	TCCGGA	1 cut(s)	1013
MroXI	GAANNNNTTC	2 cut(s)	1317, 1485
MslI	CAYNNNNRTG	3 cut(s)	269, 1164, 1986
MspA1I	CMGCKG	3 cut(s)	713, 960, 1064
MspI	CCGG	5 cut(s)	40, 53, 883, 1014, 1823
MspR9I	CCNGG	6 cut(s)	40, 41, 53, 175, 927, 1806
Mva1269I	GAATGC	1 cut(s)	89
MvaI	CCWGG	3 cut(s)	175, 927, 1806
NciI	CCSGG	3 cut(s)	40, 41, 53
NcoI	CCATGG	1 cut(s)	12
NdeII	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
NlaIV	GGNNCC	9 cut(s)	9, 50, 57, 237, 881, 1344, 1560, 1728, 1845
NmuCI	GTSAC	2 cut(s)	1778, 2117
NsbI	TGCGCA	1 cut(s)	1787
NspI	RCATGY	4 cut(s)	1090, 1575, 1699, 1786
OliI	CACNNNNGTG	1 cut(s)	269
PaeI	GCATGC	1 cut(s)	1575
PceI	AGGCCT	1 cut(s)	250
PctI	GAATGC	1 cut(s)	89
PdmI	GAANNNNTTC	2 cut(s)	1317, 1485
PfeI	GAWTC	2 cut(s)	886, 1027
PflMI	CCANNNNNTGG	1 cut(s)	2139
PfoI	TCCNGGA	1 cut(s)	51
PkrI	GCNGC	6 cut(s)	89, 973, 1237, 1459, 1790, 1927
PleI	GAGTC	2 cut(s)	1791, 2247
PpsI	GAGTC	2 cut(s)	1791, 2247
PshBI	ATTAAT	1 cut(s)	1932
Psp6I	CCWGG	3 cut(s)	173, 925, 1804
PspFI	CCCAGC	2 cut(s)	697, 1722
PspGI	CCWGG	3 cut(s)	173, 925, 1804
PspN4I	GGNNCC	9 cut(s)	9, 50, 57, 237, 881, 1344, 1560, 1728, 1845
PspPI	GGNCC	3 cut(s)	7, 16, 923
PsrI	GAACNNNNNNTAC	2 cut(s)	1372, 1404
PsuI	RGATCY	2 cut(s)	48, 1342
PvuII	CAGCTG	3 cut(s)	713, 960, 1064
RsaI	GTAC	2 cut(s)	1137, 1548
RsaNI	GTAC	2 cut(s)	1136, 1547
RseI	CAYNNNNRTG	3 cut(s)	269, 1164, 1986
SatI	GCNGC	6 cut(s)	88, 972, 1236, 1458, 1789, 1926
Sau3AI	GATC	9 cut(s)	26, 48, 110, 603, 715, 1156, 1342, 1375, 2038
Sau96I	GGNCC	3 cut(s)	7, 16, 923
SchI	GAGTC	2 cut(s)	1791, 2247
ScrFI	CCNGG	6 cut(s)	40, 41, 53, 175, 927, 1806
SduI	GDGCHC	1 cut(s)	1731
SexAI	ACCWGGT	2 cut(s)	925, 1804
SfcI	CTRYAG	2 cut(s)	276, 1074
SinI	GGWCC	3 cut(s)	7, 16, 923
SmaI	CCCGGG	1 cut(s)	41
SmiMI	CAYNNNNRTG	3 cut(s)	269, 1164, 1986
SmlI	CTYRAG	1 cut(s)	2016
SmoI	CTYRAG	1 cut(s)	2016
SphI	GCATGC	1 cut(s)	1575
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	4 cut(s)	67, 1514, 1830, 2033
SspMI	CTAG	2 cut(s)	1565, 1739
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	6 cut(s)	38, 39, 51, 173, 925, 1804
StyI	CCWWGG	4 cut(s)	12, 1278, 1553, 2379
TaaI	ACNGT	3 cut(s)	1075, 1916, 2028
TaiI	ACGT	2 cut(s)	1552, 1940
TaqI	TCGA	4 cut(s)	99, 285, 378, 2124
TfiI	GAWTC	2 cut(s)	886, 1027
TscAI	CASTG	9 cut(s)	940, 1078, 1540, 1627, 1919, 2033, 2092, 2106, 2206
TseFI	GTSAC	2 cut(s)	1778, 2117
TseI	GCWGC	6 cut(s)	87, 971, 1235, 1457, 1788, 1925
Tsp45I	GTSAC	2 cut(s)	1778, 2117
TspGWI	ACGGA	1 cut(s)	2356
TspMI	CCCGGG	1 cut(s)	39
TspRI	CASTG	9 cut(s)	940, 1078, 1540, 1627, 1919, 2033, 2092, 2106, 2206
Van91I	CCANNNNNTGG	1 cut(s)	2139
VpaK11BI	GGWCC	3 cut(s)	7, 16, 923
VspI	ATTAAT	1 cut(s)	1932
XapI	RAATTY	2 cut(s)	499, 1250
XbaI	TCTAGA	1 cut(s)	1564
XceI	RCATGY	4 cut(s)	1090, 1575, 1699, 1786
XmaI	CCCGGG	1 cut(s)	39
XmiI	GTMKAC	1 cut(s)	1077
XmnI	GAANNNNTTC	2 cut(s)	1317, 1485
XspI	CTAG	2 cut(s)	1565, 1739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.