AT3G21865

PEX22 PEX22 (peroxin 22)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016180
Physical Location & Seq
Reverse (-)
7701003 .. 7703504
2502 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21865.1

Sequence Viewer

Length: 852 bp
ATGGCGGAATCGTCGTCGCCGTCGCCTACTGAAGAGATCGTTCGGTTGATTAAGCGTTTAAGCGCTTACGTTGCTTTCAAAATGTCAAGCCTTTTCTCCACTACATCGATTCGAAATCTGGATTCAAGATCAATTGGTGCGATTGCGGGGCTTGCGATTGCGGTGATATTTACGTGGAGGGCGATAAGAACGCCCGGAGAGCAACGTCAGAGAAGGCAGCCGAAGCGTAGGATTCACAATGCTGAAACGTCTAGTGCTGCTGCTGCTGCTTCTCAGTCGAATCTAGCTTCTTCGGTTGCTCCGGAAGTTTCTTCGCCTCGTGAGGATAATGCGGTGCAAGATGTTGTTGATCAGTTCTTTCAACCTGTTAAACCTACTTTGGGGCAAATAGTTAGACAGAAGCTTAGTGAAGGAAGGAAGGTAACATGCCGTCTTCTTGGAGTAATTCTTGAGGAAACAAGTCCAGAAGAACTCCAAAAACAAGCCACAGTGAGGTCATCTGTTTTGGAAGTTCTACTAGAGATTACAAAGTATAGTGATTTGTATCTTATGGAAAGAGTTCTTGACGATGAAAGCGAGGCCAAAGTTTTACAGGCTTTAGAGAATGCAGGAGTTTTTACATCTGGTGGTTTAGTCAAAGATAAGGTGCTCTTTTGTAGTACAGAGATAGGAAGAACTTCCTTTGTCAGACAGCTAGAACCTGACTGGCATATTGATACAAACCCGGAAATCAGCACACAACTAGCTAGGTTCATCAAATATCAGCTTCATGTCGCTACGGTGAAACCCGAGCGAACAGCTCCAAATGTGTTCACCTCGCAGTCAATAGAACAGTTCTTTGGAAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007031 GO:0008150 GO:0009987 GO:0016043 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

31.4

Weight (kDa)

7.76

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_Pex22 PF22978 129 - 280 1.5e-44 Peroxisome biogenesis protein 22, Haloacid dehydrogenase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21865
fragaria_vesca FvH4_6g43550 FvH4_6g43550
malus_domestica MD09G1105000.v1.1 MD17G1092700.v1.1
prunus_persica Prupe.3G220500_v2.0.a1 Prupe.3G220500_v2.0.a1
pyrus_communis pycom09g02810 pycom17g09030
rosa_chinensis RchiOBHm_Chr2g0160081
rosa_laevigata RLG00000021196
rosa_rugosa Rorug02G0480900
rosa_samantha Rh2AG546800 Rh2BG560200
rosa_wichuraiana Rw2G045220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 4 cut(s) 5, 146, 161, 332
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 1 cut(s) 661
AfeI AGCGCT 1 cut(s) 64
AfiI CCNNNNNNNGG 2 cut(s) 380, 492
AgsI TTSAA 3 cut(s) 79, 126, 362
AjuI GAANNNNNNNTTGG 1 cut(s) 822
AluBI AGCT 6 cut(s) 287, 403, 694, 746, 766, 800
AluI AGCT 6 cut(s) 287, 403, 694, 746, 766, 800
Alw21I GWGCWC 1 cut(s) 651
Ama87I CYCGRG 1 cut(s) 788
Aor13HI TCCGGA 1 cut(s) 301
Aor51HI AGCGCT 1 cut(s) 64
AoxI GGCC 1 cut(s) 579
ApeKI GCWGC 5 cut(s) 217, 257, 260, 263, 266
Asp700I GAANNNNTTC 2 cut(s) 558, 676
AspLEI GCGC 1 cut(s) 65
AsuC2I CCSGG 2 cut(s) 195, 725
AsuHPI GGTGA 3 cut(s) 175, 793, 805
AsuII TTCGAA 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 788
BauI CACGAG 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 5 cut(s) 229, 244, 247, 250, 253
BceAI ACGGC 2 cut(s) 4, 414
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 2 cut(s) 195, 725
BfaI CTAG 6 cut(s) 252, 284, 518, 695, 743, 747
BfoI RGCGCY 1 cut(s) 66
BisI GCNGC 5 cut(s) 218, 258, 261, 264, 267
BlsI GCNGC 5 cut(s) 219, 259, 262, 265, 268
Bme1390I CCNGG 2 cut(s) 195, 725
BmeT110I CYCGRG 1 cut(s) 788
BmrFI CCNGG 2 cut(s) 195, 725
BpiI GAAGAC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 2 cut(s) 195, 725
Bsa29I ATCGAT 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 380, 492
Bse1I ACTGG 1 cut(s) 710
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 2 cut(s) 380, 492
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 710
BseXI GCAGC 5 cut(s) 229, 244, 247, 250, 253
BshFI GGCC 1 cut(s) 581
BshVI ATCGAT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 651
BsiHKCI CYCGRG 1 cut(s) 788
BsiSI CCGG 3 cut(s) 195, 302, 725
BslI CCNNNNNNNGG 2 cut(s) 380, 492
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 1 cut(s) 581
BsoBI CYCGRG 1 cut(s) 788
Bsp119I TTCGAA 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 651
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 3 cut(s) 36, 128, 349
BspACI CCGC 4 cut(s) 5, 146, 161, 332
BspANI GGCC 1 cut(s) 581
BspCNI CTCAG 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 107
BspEI TCCGGA 1 cut(s) 301
BspT104I TTCGAA 1 cut(s) 112
BsrI ACTGG 1 cut(s) 710
BssMI GATC 3 cut(s) 36, 128, 349
BssSI CACGAG 1 cut(s) 318
Bst2BI CACGAG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 490, 781, 834
Bst6I CTCTTC 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 2 cut(s) 273, 404
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 3 cut(s) 39, 131, 352
BstMBI GATC 3 cut(s) 36, 128, 349
BstMWI GCNNNNNNNGC 6 cut(s) 71, 152, 199, 223, 263, 266
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 2 cut(s) 193, 723
BstV1I GCAGC 5 cut(s) 229, 244, 247, 250, 253
BstV2I GAAGAC 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 107
BsuRI GGCC 1 cut(s) 581
BsuTUI ATCGAT 1 cut(s) 107
BtsIMutI CAGTG 1 cut(s) 495
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 107
Csp6I GTAC 1 cut(s) 660
CviAII CATG 2 cut(s) 426, 770
CviQI GTAC 1 cut(s) 660
DdeI CTNAG 2 cut(s) 273, 404
DpnI GATC 3 cut(s) 38, 130, 351
DpnII GATC 3 cut(s) 36, 128, 349
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 20
Eco47III AGCGCT 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 788
FaeI CATG 2 cut(s) 429, 773
FaiI YATR 5 cut(s) 427, 534, 551, 711, 771
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FatI CATG 2 cut(s) 425, 769
FauI CCCGC 1 cut(s) 139
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 5 cut(s) 218, 258, 261, 264, 267
Fsp4HI GCNGC 5 cut(s) 218, 258, 261, 264, 267
FspBI CTAG 6 cut(s) 252, 284, 518, 695, 743, 747
GlaI GCGC 1 cut(s) 64
GluI GCNGC 5 cut(s) 218, 258, 261, 264, 267
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 1 cut(s) 581
HapII CCGG 3 cut(s) 195, 302, 725
HhaI GCGC 1 cut(s) 65
Hin1II CATG 2 cut(s) 429, 773
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 5 cut(s) 8, 109, 122, 232, 280
HpaII CCGG 3 cut(s) 195, 302, 725
HphI GGTGA 3 cut(s) 175, 793, 805
Hpy166II GTNNAC 1 cut(s) 813
Hpy188I TCNGA 2 cut(s) 210, 689
Hpy188III TCNNGA 7 cut(s) 119, 126, 302, 320, 449, 464, 563
Hpy8I GTNNAC 1 cut(s) 813
Hpy99I CGWCG 3 cut(s) 16, 19, 25
HpyAV CCTTC 4 cut(s) 207, 404, 408, 412
HpyCH4III ACNGT 3 cut(s) 490, 781, 834
HpyCH4IV ACGT 4 cut(s) 69, 173, 205, 248
HpyCH4V TGCA 2 cut(s) 337, 608
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 152, 199, 223, 263, 266
HpyF3I CTNAG 2 cut(s) 273, 404
HpySE526I ACGT 4 cut(s) 69, 173, 205, 248
Hsp92II CATG 2 cut(s) 429, 773
HspAI GCGC 1 cut(s) 63
Kpn2I TCCGGA 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 3 cut(s) 36, 128, 349
LmnI GCTCC 2 cut(s) 304, 805
Lsp1109I GCAGC 5 cut(s) 229, 244, 247, 250, 253
MaeI CTAG 6 cut(s) 252, 284, 518, 695, 743, 747
MaeII ACGT 4 cut(s) 69, 173, 205, 248
MaeIII GTNAC 1 cut(s) 421
MalI GATC 3 cut(s) 38, 130, 351
MboI GATC 3 cut(s) 36, 128, 349
MboII GAAGA 6 cut(s) 44, 282, 303, 425, 479, 684
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 2 cut(s) 132, 444
MnlI CCTC 7 cut(s) 171, 316, 327, 445, 486, 571, 826
MroI TCCGGA 1 cut(s) 301
MroXI GAANNNNTTC 2 cut(s) 558, 676
MseI TTAA 4 cut(s) 51, 59, 369, 850
MspI CCGG 3 cut(s) 195, 302, 725
MspR9I CCNGG 2 cut(s) 195, 725
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 610
MwoI GCNNNNNNNGC 6 cut(s) 71, 152, 199, 223, 263, 266
NciI CCSGG 2 cut(s) 195, 725
NdeII GATC 3 cut(s) 36, 128, 349
NlaIII CATG 2 cut(s) 429, 773
NspI RCATGY 1 cut(s) 429
NspV TTCGAA 1 cut(s) 112
PcsI WCGNNNNNNNCGW 3 cut(s) 17, 179, 573
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 2 cut(s) 558, 676
PfeI GAWTC 5 cut(s) 8, 109, 122, 232, 280
PkrI GCNGC 5 cut(s) 219, 259, 262, 265, 268
Ppu21I YACGTR 1 cut(s) 174
RsaI GTAC 1 cut(s) 661
RsaNI GTAC 1 cut(s) 660
SaqAI TTAA 4 cut(s) 51, 59, 369, 850
SatI GCNGC 5 cut(s) 218, 258, 261, 264, 267
Sau3AI GATC 3 cut(s) 36, 128, 349
ScrFI CCNGG 2 cut(s) 195, 725
SduI GDGCHC 1 cut(s) 651
SfuI TTCGAA 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 132, 444
SsiI CCGC 4 cut(s) 5, 146, 161, 332
SspMI CTAG 6 cut(s) 252, 284, 518, 695, 743, 747
StyD4I CCNGG 2 cut(s) 193, 723
TaaI ACNGT 3 cut(s) 490, 781, 834
TaiI ACGT 4 cut(s) 72, 176, 208, 251
TaqI TCGA 3 cut(s) 107, 112, 278
TasI AATT 2 cut(s) 132, 444
TatI WGTACW 1 cut(s) 659
TfiI GAWTC 5 cut(s) 8, 109, 122, 232, 280
Tru1I TTAA 4 cut(s) 51, 59, 369, 850
Tru9I TTAA 4 cut(s) 51, 59, 369, 850
TscAI CASTG 1 cut(s) 495
TseI GCWGC 5 cut(s) 217, 257, 260, 263, 266
TspDTI ATGAA 3 cut(s) 585, 742, 758
TspRI CASTG 1 cut(s) 495
XceI RCATGY 1 cut(s) 429
XmnI GAANNNNTTC 2 cut(s) 558, 676
XspI CTAG 6 cut(s) 252, 284, 518, 695, 743, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.