AT3G24560

PP-loop family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008644
Physical Location & Seq
Forward (+)
8963770 .. 8966616
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24560.2

Sequence Viewer

Length: 1389 bp
ATGCAGATGACCAGTTGGATGCAGGCTGAGTTATTCATTCTCAGGTTATCTCGTAGTAGCGGTGTACTTGGTCTTGCGGGTACAGCTTTTGCTTCCGAGATTTTCTCCAGAAATCTGCAGTTAGATGCAAAACATATGAAGAACCAATCTATTCGCTTAGTGCGTCCGCTACTAGATTTATGGAAAGAAGACATGTACAAGATATGTCAATGGGGTAGACAAGATTGGGTTGAAGATCCAACTAATCGTAGTCAGTTGTTTGTTCGGAATAGGATTCGAACATCAATAGGAAATCTAGAATCAGGTAGCTTCAAGTCAGAGCTGCAAGCAGTCATTTCTGAATGCAGGAGAACACGTTCATTTGTTGATAAAGTTTGTACAGACTTAATACATCAGACCGTAACAGTGACAGATAAAGGGTATGCTATTCTTGATCTAGAGAGACTTAATCCTTCAGGAGTTAAAGACATTTGCCTCTCAAAGTATCTCTTTGCGGTCCTGCAGTTTATCTCTCAACGGCAAAGACCAATCAGAGGAAACACTTCAAAGTTGCTTTTGAACTATATCCGCGCAATTCCGTGCAGGACATCTCTAACAGCTGCTGGTTGCTACCTTAGTCCTGCTCCAGGGTCGAAGGGTACCAAAATTATAGTCTCATGTTCTGTTGACTGTCCTTTACCATCAAAGACAGAACTCTTGAACATTTCCTTCAATGAAACTCCATCTGATGACCTTGGACAAATCATAGCAGACGCAAAATCGTTTTCTGACCATGTGGCTCCAACCAGCTTGTTTGAAGTCCAGTTTCTGGATGTTGCATCTGAATCCGTATTGAGTAAAGCCAGAGAGCTCAATCTTCTCAGCGAATCGACGTATACAACCATTGGTCTACTGCAGAGGGATGAAACTAAACGGTTTTTAACTAAAACAGAAGAGAAATCAGTGGAATTAGAGCATGGAACCAACATTGCAGCTTCCTCTGATAAAGTACATCTTTGTCTAGGGCAAAATCTCTACTTCATGAACCGTTTTCTCATCAGATGGAACCTAAGTGACCACCAATGTAATGAAGCAGACTGCAGAAATTGTCCAGTGAGTACTGCTACATCAATGGAGGTTCGGCACATGGTTGAACCCGACTGGTTATATCTAGCTGAGCTTTCAAAATGCTCAACCTCAAATCATTCTATTTCATCCTCACAAAAAGCTCTCAGGTCCTTAAAGTTGATCCCAGCCGCTGCAAGAAAAAGCCTTCCTGTCTTGGTCAATCACTGTGGATTACTACTCTGCATACCAGCTATTGGCTTCAGTTATTGTTCATGCCTTGAGGCATCTGCAGTGTTTCTTCCAAGAATACCACTCGGAGGCGGTCATAGCTCATTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATP_bind_3

Protein Analysis

462

Amino Acids

51.56

Weight (kDa)

8.6

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP_bind_3 PF01171 87 - 292 2.3e-54 PP-loop domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 638
AccB7I CCANNNNNTGG 2 cut(s) 808, 1301
AccI GTMKAC 3 cut(s) 217, 875, 889
AccII CGCG 1 cut(s) 570
AciI CCGC 7 cut(s) 60, 77, 167, 494, 568, 1236, 1368
AclWI GGATC 2 cut(s) 230, 1222
AcuI CTGAAG 2 cut(s) 438, 1291
AfaI GTAC 7 cut(s) 66, 82, 197, 379, 640, 990, 1099
AfiI CCNNNNNNNGG 5 cut(s) 533, 626, 808, 1301, 1364
AflIII ACRYGT 2 cut(s) 192, 353
AgsI TTSAA 9 cut(s) 233, 313, 546, 559, 700, 712, 797, 1133, 1164
AjnI CCWGG 1 cut(s) 625
AloI GAACNNNNNNTCC 2 cut(s) 340, 372
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 2 cut(s) 436, 658
AlwI GGATC 2 cut(s) 230, 1222
AlwNI CAGNNNCTG 3 cut(s) 602, 808, 1238
ApeKI GCWGC 4 cut(s) 322, 599, 971, 1238
ArsI GACNNNNNNTTYG 2 cut(s) 636, 668
Asp700I GAANNNNTTC 2 cut(s) 355, 541
Asp718I GGTACC 1 cut(s) 638
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 2 cut(s) 496, 1215
AsuII TTCGAA 1 cut(s) 277
AvaII GGWCC 2 cut(s) 496, 1215
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 309, 586, 983, 1225
BccI CCATC 3 cut(s) 688, 730, 1035
BceAI ACGGC 1 cut(s) 533
BciT130I CCWGG 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 436, 658
BfaI CTAG 6 cut(s) 173, 296, 437, 1001, 1151, 1387
BfmI CTRYAG 5 cut(s) 116, 500, 893, 1078, 1335
BisI GCNGC 5 cut(s) 323, 600, 972, 1236, 1239
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 5 cut(s) 324, 601, 973, 1237, 1240
BmcAI AGTACT 1 cut(s) 1099
Bme1390I CCNGG 1 cut(s) 627
Bme18I GGWCC 2 cut(s) 496, 1215
BmgT120I GGNCC 2 cut(s) 496, 1215
BmiI GGNNCC 4 cut(s) 640, 780, 961, 1046
BmrFI CCNGG 1 cut(s) 627
BmsI GCATC 4 cut(s) 9, 115, 827, 1340
BpiI GAAGAC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 89, 121
BpmI CTGGAG 2 cut(s) 91, 609
Bpu1102I GCTNAGC 1 cut(s) 1155
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 1346
BsaJI CCNNGG 2 cut(s) 626, 733
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bsc4I CCNNNNNNNGG 5 cut(s) 533, 626, 808, 1301, 1364
Bse1I ACTGG 4 cut(s) 12, 802, 1091, 1145
Bse3DI GCAATG 1 cut(s) 966
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 2 cut(s) 626, 733
BseGI GGATG 4 cut(s) 24, 817, 907, 1193
BseLI CCNNNNNNNGG 5 cut(s) 533, 626, 808, 1301, 1364
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 5 cut(s) 18, 55, 874, 1146, 1225
BseNI ACTGG 4 cut(s) 12, 802, 1091, 1145
BseXI GCAGC 4 cut(s) 309, 586, 983, 1225
BseYI CCCAGC 1 cut(s) 1231
BsgI GTGCAG 1 cut(s) 601
Bsh1236I CGCG 1 cut(s) 570
BshNI GGYRCC 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 852
BslI CCNNNNNNNGG 5 cut(s) 533, 626, 808, 1301, 1364
BsmAI GTCTC 2 cut(s) 436, 658
BsmI GAATGC 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 852
Bsp1407I TGTACA 2 cut(s) 195, 377
Bsp143I GATC 3 cut(s) 235, 433, 1227
Bsp1720I GCTNAGC 1 cut(s) 1155
BspACI CCGC 7 cut(s) 60, 77, 167, 494, 568, 1236, 1368
BspCNI CTCAG 5 cut(s) 19, 54, 873, 1147, 1224
BspFNI CGCG 1 cut(s) 570
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 4 cut(s) 640, 780, 961, 1046
BspMAI CTGCAG 5 cut(s) 120, 504, 897, 1082, 1339
BspPI GGATC 2 cut(s) 230, 1222
BspT104I TTCGAA 1 cut(s) 277
BspT107I GGYRCC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 966
BsrGI TGTACA 2 cut(s) 195, 377
BsrI ACTGG 4 cut(s) 12, 802, 1091, 1145
BssECI CCNNGG 2 cut(s) 626, 733
BssMI GATC 3 cut(s) 235, 433, 1227
BssNAI GTATAC 1 cut(s) 876
BssT1I CCWWGG 1 cut(s) 733
Bst1107I GTATAC 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 6 cut(s) 400, 406, 671, 915, 1028, 1274
Bst6I CTCTTC 1 cut(s) 927
BstAUI TGTACA 2 cut(s) 195, 377
BstBI TTCGAA 1 cut(s) 277
BstC8I GCNNGC 2 cut(s) 24, 327
BstDEI CTNAG 8 cut(s) 27, 41, 157, 614, 860, 1049, 1155, 1211
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 4 cut(s) 24, 817, 907, 1193
BstFNI CGCG 1 cut(s) 570
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 3 cut(s) 238, 436, 1230
BstMAI GTCTC 2 cut(s) 436, 658
BstMBI GATC 3 cut(s) 235, 433, 1227
BstMWI GCNNNNNNNGC 2 cut(s) 83, 1374
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 1 cut(s) 625
BstSFI CTRYAG 5 cut(s) 116, 500, 893, 1078, 1335
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 4 cut(s) 309, 586, 983, 1225
BstV2I GAAGAC 1 cut(s) 195
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 876
BtsCI GGATG 4 cut(s) 24, 817, 907, 1193
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 5 cut(s) 411, 948, 1098, 1270, 1344
Cac8I GCNNGC 2 cut(s) 24, 327
CaiI CAGNNNCTG 3 cut(s) 602, 808, 1238
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 1 cut(s) 572
Cfr13I GGNCC 2 cut(s) 496, 1215
CseI GACGC 2 cut(s) 152, 761
Csp6I GTAC 7 cut(s) 65, 81, 196, 378, 639, 989, 1098
CspCI CAANNNNNGTGG 2 cut(s) 1255, 1290
CviAII CATG 7 cut(s) 193, 657, 773, 956, 1021, 1126, 1320
CviQI GTAC 7 cut(s) 65, 81, 196, 378, 639, 989, 1098
DdeI CTNAG 8 cut(s) 27, 41, 157, 614, 860, 1049, 1155, 1211
DpnI GATC 3 cut(s) 237, 435, 1229
DpnII GATC 3 cut(s) 235, 433, 1227
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 852
Eco47I GGWCC 2 cut(s) 496, 1215
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 2 cut(s) 438, 1291
EcoICRI GAGCTC 1 cut(s) 850
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 1215
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 7 cut(s) 196, 660, 776, 959, 1024, 1129, 1323
FalI AAGNNNNNCTT 4 cut(s) 473, 505, 978, 1010
FatI CATG 7 cut(s) 192, 656, 772, 955, 1020, 1125, 1319
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 135
FblI GTMKAC 3 cut(s) 217, 875, 889
Fnu4HI GCNGC 5 cut(s) 323, 600, 972, 1236, 1239
FokI GGATG 4 cut(s) 31, 824, 914, 1180
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 5 cut(s) 323, 600, 972, 1236, 1239
FspBI CTAG 6 cut(s) 173, 296, 437, 1001, 1151, 1387
GlaI GCGC 1 cut(s) 571
GluI GCNGC 5 cut(s) 323, 600, 972, 1236, 1239
GsaI CCCAGC 1 cut(s) 1235
GsuI CTGGAG 2 cut(s) 91, 609
HgaI GACGC 2 cut(s) 152, 761
HhaI GCGC 1 cut(s) 572
Hin1II CATG 7 cut(s) 196, 660, 776, 959, 1024, 1129, 1323
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HinfI GANTC 4 cut(s) 274, 299, 824, 866
Hpy166II GTNNAC 5 cut(s) 65, 218, 667, 876, 890
Hpy188III TCNNGA 8 cut(s) 108, 296, 431, 437, 456, 697, 809, 1021
Hpy8I GTNNAC 5 cut(s) 65, 218, 667, 876, 890
Hpy99I CGWCG 1 cut(s) 874
HpyAV CCTTC 4 cut(s) 462, 628, 718, 1262
HpyCH4III ACNGT 6 cut(s) 400, 406, 671, 915, 1028, 1274
HpyCH4IV ACGT 2 cut(s) 355, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 1374
HpyF3I CTNAG 8 cut(s) 27, 41, 157, 614, 860, 1049, 1155, 1211
HpySE526I ACGT 2 cut(s) 355, 872
Hsp92II CATG 7 cut(s) 196, 660, 776, 959, 1024, 1129, 1323
HspAI GCGC 1 cut(s) 570
KpnI GGTACC 1 cut(s) 642
Kzo9I GATC 3 cut(s) 235, 433, 1227
LmnI GCTCC 2 cut(s) 628, 784
Lsp1109I GCAGC 4 cut(s) 309, 586, 983, 1225
LweI GCATC 4 cut(s) 9, 115, 827, 1340
MaeI CTAG 6 cut(s) 173, 296, 437, 1001, 1151, 1387
MaeII ACGT 2 cut(s) 355, 872
MaeIII GTNAC 3 cut(s) 400, 406, 1052
MalI GATC 3 cut(s) 237, 435, 1229
MboI GATC 3 cut(s) 235, 433, 1227
MboII GAAGA 6 cut(s) 151, 200, 245, 848, 944, 1337
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 852
MluCI AATT 4 cut(s) 573, 645, 947, 1084
MmeI TCCRAC 2 cut(s) 263, 806
MnlI CCTC 9 cut(s) 485, 527, 891, 988, 1108, 1186, 1207, 1321, 1358
MroXI GAANNNNTTC 2 cut(s) 355, 541
MseI TTAA 5 cut(s) 386, 447, 462, 920, 1220
MspA1I CMGCKG 2 cut(s) 599, 1238
MspR9I CCNGG 1 cut(s) 627
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 2 cut(s) 83, 1374
NdeI CATATG 1 cut(s) 135
NdeII GATC 3 cut(s) 235, 433, 1227
NlaIII CATG 7 cut(s) 196, 660, 776, 959, 1024, 1129, 1323
NlaIV GGNNCC 4 cut(s) 640, 780, 961, 1046
NmuCI GTSAC 2 cut(s) 406, 1052
NspI RCATGY 1 cut(s) 196
NspV TTCGAA 1 cut(s) 277
PagI TCATGA 1 cut(s) 1020
PciI ACATGT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PctI GAATGC 1 cut(s) 347
PdmI GAANNNNTTC 2 cut(s) 355, 541
PfeI GAWTC 4 cut(s) 274, 299, 824, 866
PflMI CCANNNNNTGG 2 cut(s) 808, 1301
PkrI GCNGC 5 cut(s) 324, 601, 973, 1237, 1240
PpuMI RGGWCCY 1 cut(s) 1215
PscI ACATGT 1 cut(s) 192
Psp124BI GAGCTC 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 1215
Psp6I CCWGG 1 cut(s) 625
PspFI CCCAGC 1 cut(s) 1231
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 4 cut(s) 640, 780, 961, 1046
PspPI GGNCC 2 cut(s) 496, 1215
PspPPI RGGWCCY 1 cut(s) 1215
PstI CTGCAG 5 cut(s) 120, 504, 897, 1082, 1339
PstNI CAGNNNCTG 3 cut(s) 602, 808, 1238
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 7 cut(s) 66, 82, 197, 379, 640, 990, 1099
RsaNI GTAC 7 cut(s) 65, 81, 196, 378, 639, 989, 1098
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 5 cut(s) 386, 447, 462, 920, 1220
SatI GCNGC 5 cut(s) 323, 600, 972, 1236, 1239
Sau3AI GATC 3 cut(s) 235, 433, 1227
Sau96I GGNCC 2 cut(s) 496, 1215
ScaI AGTACT 1 cut(s) 1099
ScrFI CCNGG 1 cut(s) 627
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 4 cut(s) 9, 115, 827, 1340
SfcI CTRYAG 5 cut(s) 116, 500, 893, 1078, 1335
SfuI TTCGAA 1 cut(s) 277
SinI GGWCC 2 cut(s) 496, 1215
SmlI CTYRAG 1 cut(s) 1325
SmoI CTYRAG 1 cut(s) 1325
Sse9I AATT 4 cut(s) 573, 645, 947, 1084
SsiI CCGC 7 cut(s) 60, 77, 167, 494, 568, 1236, 1368
SspMI CTAG 6 cut(s) 173, 296, 437, 1001, 1151, 1387
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 6 cut(s) 400, 406, 671, 915, 1028, 1274
TaiI ACGT 2 cut(s) 358, 875
TaqI TCGA 3 cut(s) 277, 632, 869
TasI AATT 4 cut(s) 573, 645, 947, 1084
TatI WGTACW 5 cut(s) 64, 195, 377, 988, 1097
TauI GCSGC 1 cut(s) 1238
TfiI GAWTC 4 cut(s) 274, 299, 824, 866
Tru1I TTAA 5 cut(s) 386, 447, 462, 920, 1220
Tru9I TTAA 5 cut(s) 386, 447, 462, 920, 1220
TscAI CASTG 5 cut(s) 411, 948, 1098, 1277, 1344
TseFI GTSAC 2 cut(s) 406, 1052
TseI GCWGC 4 cut(s) 322, 599, 971, 1238
Tsp45I GTSAC 2 cut(s) 406, 1052
TspGWI ACGGA 2 cut(s) 567, 817
TspRI CASTG 5 cut(s) 411, 948, 1098, 1277, 1344
Van91I CCANNNNNTGG 2 cut(s) 808, 1301
VpaK11BI GGWCC 2 cut(s) 496, 1215
XagI CCTNNNNNAGG 1 cut(s) 624
XbaI TCTAGA 2 cut(s) 295, 436
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 3 cut(s) 217, 875, 889
XmnI GAANNNNTTC 2 cut(s) 355, 541
XspI CTAG 6 cut(s) 173, 296, 437, 1001, 1151, 1387
ZrmI AGTACT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.