AT3G25120

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015193
Physical Location & Seq
Reverse (-)
9149528 .. 9151334
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25120.1

Sequence Viewer

Length: 570 bp
ATGGCGTTGGGTGATCGGAAATCCCCAGAACAAACAAATCAGGCGTTATCTCCTCCGACGCCTATTGTGCAGGAAAATGGAACTCCGACGAAGCGTGTGTTGATCACTTCCCTTTTAGCAGGAGTAATTGGTGGAGGAGCTGGTTTAGTGTCTAAACACCGGATAGCTCATCCCAATATTCCTACTGTTTACGCTGCTAATTTTGCTATTGTCGCCGGTTGCTATTGCGGAGCTCGTGAATCTGTGAGAATAACTCGAAGATCAGAACACGATGATTTAATGAACTCAGCTATTGGAGGACTTTTTAGTGGTGCTTTGCTTGGAAGACTTCAAGGAGGTCCTAAGGGTGCGATTCGCTACTCTCTAGTTTTTGCTGCTGTAGGCACAGCATTTGATTATGCTACCCTTAAAGGAAAACCAATGTTAGAGAGCTACCGTAACATGGAGTCATTCAAGTTACCTGAATGGTCTCCTATTAAAGTCCTCGACGAAGAAGCCTTAGCAAAGAAGAAAGCTCATGAAGAGAAGATATTCCCTGAAAGAGTCCTCGGCAAATTGAACAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

189

Amino Acids

20.37

Weight (kDa)

9.49

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 64 - 127 1.1e-08 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 442
AgsI TTSAA 3 cut(s) 332, 454, 559
AluBI AGCT 6 cut(s) 140, 167, 233, 290, 432, 515
AluI AGCT 6 cut(s) 140, 167, 233, 290, 432, 515
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 1 cut(s) 474
ApeKI GCWGC 2 cut(s) 194, 374
Asp700I GAANNNNTTC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 23
AvaII GGWCC 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 342
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 331
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 2 cut(s) 181, 361
BclI TGATCA 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 474
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 1 cut(s) 378
BisI GCNGC 2 cut(s) 195, 375
BlsI GCNGC 2 cut(s) 196, 376
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 331
BplI GAGNNNNNCTC 2 cut(s) 238, 270
Bpu10I CCTNAGC 1 cut(s) 499
BsaHI GRCGYC 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 547
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 215
Bse21I CCTNAGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 547
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMII CTCAG 1 cut(s) 300
BseRI GAGGAG 2 cut(s) 42, 150
BseXI GCAGC 2 cut(s) 181, 361
BsgI GTGCAG 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 235
BsiSI CCGG 2 cut(s) 160, 216
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 3 cut(s) 13, 102, 260
BspACI CCGC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 299
BspHI TCATGA 1 cut(s) 517
BspTNI GGTCTC 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 1 cut(s) 547
BssMI GATC 3 cut(s) 13, 102, 260
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 187, 437
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 59
BstDEI CTNAG 3 cut(s) 286, 342, 499
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 3 cut(s) 16, 105, 263
BstMAI GTCTC 1 cut(s) 474
BstMBI GATC 3 cut(s) 13, 102, 260
BstMWI GCNNNNNNNGC 3 cut(s) 67, 203, 212
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 181, 361
BstV2I GAAGAC 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 169
CciI TCATGA 1 cut(s) 517
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 338
CseI GACGC 1 cut(s) 67
CviAII CATG 2 cut(s) 442, 518
CviJI RGCY 7 cut(s) 140, 167, 233, 290, 432, 497, 515
CviKI_1 RGCY 7 cut(s) 140, 167, 233, 290, 432, 497, 515
DdeI CTNAG 3 cut(s) 286, 342, 499
DpnI GATC 3 cut(s) 15, 104, 262
DpnII GATC 3 cut(s) 13, 102, 260
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 233
Eco81I CCTNAGG 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 2 cut(s) 445, 521
FaiI YATR 3 cut(s) 399, 443, 519
FatI CATG 2 cut(s) 441, 517
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 195, 375
FokI GGATG 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 195, 375
FspBI CTAG 1 cut(s) 365
GluI GCNGC 2 cut(s) 195, 375
HapII CCGG 2 cut(s) 160, 216
HgaI GACGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 2 cut(s) 445, 521
HinfI GANTC 4 cut(s) 239, 352, 446, 543
HpaII CCGG 2 cut(s) 160, 216
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 4 cut(s) 18, 57, 87, 265
Hpy188III TCNNGA 2 cut(s) 236, 518
Hpy8I GTNNAC 1 cut(s) 190
Hpy99I CGWCG 3 cut(s) 61, 91, 491
HpyCH4III ACNGT 2 cut(s) 187, 437
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 203, 212
HpyF3I CTNAG 3 cut(s) 286, 342, 499
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 2 cut(s) 445, 521
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 3 cut(s) 13, 102, 260
LmnI GCTCC 2 cut(s) 137, 230
LpnPI CCDG 9 cut(s) 26, 39, 56, 105, 126, 173, 229, 474, 549
Lsp1109I GCAGC 2 cut(s) 181, 361
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 2 cut(s) 437, 456
MalI GATC 3 cut(s) 15, 104, 262
MboI GATC 3 cut(s) 13, 102, 260
MboII GAAGA 6 cut(s) 270, 336, 503, 520, 533, 538
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 3 cut(s) 126, 199, 554
MlyI GAGTC 2 cut(s) 455, 552
MmeI TCCRAC 2 cut(s) 80, 110
MnlI CCTC 6 cut(s) 63, 128, 290, 329, 494, 557
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 3 cut(s) 278, 408, 477
MspI CCGG 2 cut(s) 160, 216
MwoI GCNNNNNNNGC 3 cut(s) 67, 203, 212
NdeII GATC 3 cut(s) 13, 102, 260
NlaIII CATG 2 cut(s) 445, 521
NmeAIII GCCGAG 1 cut(s) 528
PagI TCATGA 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 239, 352
PkrI GCNGC 2 cut(s) 196, 376
PleI GAGTC 2 cut(s) 454, 551
PpsI GAGTC 2 cut(s) 454, 551
PpuMI RGGWCCY 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 338
PspPI GGNCC 1 cut(s) 338
PspPPI RGGWCCY 1 cut(s) 338
SacI GAGCTC 1 cut(s) 235
SaqAI TTAA 3 cut(s) 278, 408, 477
SatI GCNGC 2 cut(s) 195, 375
Sau3AI GATC 3 cut(s) 13, 102, 260
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 2 cut(s) 455, 552
SduI GDGCHC 1 cut(s) 235
SetI ASST 8 cut(s) 142, 169, 235, 292, 340, 434, 463, 517
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 338
Sse9I AATT 3 cut(s) 126, 199, 554
SsiI CCGC 1 cut(s) 228
SspI AATATT 1 cut(s) 178
SspMI CTAG 1 cut(s) 365
SstI GAGCTC 1 cut(s) 235
TaaI ACNGT 2 cut(s) 187, 437
TaqI TCGA 2 cut(s) 256, 486
TasI AATT 3 cut(s) 126, 199, 554
TfiI GAWTC 2 cut(s) 239, 352
Tru1I TTAA 3 cut(s) 278, 408, 477
Tru9I TTAA 3 cut(s) 278, 408, 477
TseI GCWGC 2 cut(s) 194, 374
TspDTI ATGAA 2 cut(s) 296, 534
VpaK11BI GGWCC 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.