Rorug06G0160600

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015193
Physical Location & Seq
Forward (+)
22906124 .. 22911242
5119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0160600.1

Sequence Viewer

Length: 4671 bp
ATGAGGATCACTCAAATAGACGAGCTTTGCATGAGTATGACCATCGAATGTCCAGGAAAGAAACAGGAATTAAGGACTGAAGAAGATATTGATAAAGCCATCTTATGGAAGAAAGGGAGTGTTGATAAAGAGGGTAACTATTTAAGTGAGACAACCAAACAACGTGCTGAGAAAATTGATGCTTTAACGAAAGAAGTTCAAGAGGGAATTGTGTCCGATGTTGGTAGGAACGACATTTTGACACAAACTTTGGAGACGCCTAAACAATCAAGCCGTGTAAGAGGTGTTGGAGCATTTGTTACACACAAGGTATACTTCAATACATCTAGTTGCTCAGTGGACAAGGAGAAAAATGAGTCTTCTAAGGAGGAGGTATCTAGGATGGCATCCAAGAAAGAATTTGAGAAAAAAACTACTGCACAAAAGGTTCGAAAAGTGGATGACATTCCATCTCCAATTGACAGCAGTAGGGAGAGCAAGAAGGCAAAACAACAATACGATGGGGAAACCCTAGCAGAGCGCCGCTCTGAGCACCTAAACAGCGGCGAAGCAACGGCGGGCAGAGAAGGCATGGCTTTCAGGATTTTGGCATGGAATTGTCGGGGCATTGTTGGTAGCTACAGACGTAGGGCATTGATTGACATTATTAGACAGGAAAAACCTCGGATCCTATTCTTATCGGAGACCCTCTGTGATGCTAAGCAGATGGAGACTTTGCGAGTTAAAGTCGGTTTTGATAACTGTCTCTGCTCAAAGGAGGATGATTCACGTGGAGTCGCCATGTTATGGATGAATGATGTTCCTTTGCATGTCCGTAACTATTCGACGAGGCATGTGGATGTATCGATTGGTGCTAGGGGGTCGGCGGAGGAGTGGAGGCTGACGGGCATCTATGGATATGCACAGACTGGGGATCGAAGAGAGACATGGGCATTGATGAGACGACTGGCCAGTCAGTCATCCCTACCATGGCTGATCTGCGGGGATTTTAATGAGATCCTTGAAAGATCGGAGAAAACTGGAGGAGGAGAACGAAGACCGCTCCAGATGCAAGCTTTTAGGGAGTGTGTGATGGACTGCTCTCTGATCGATATGGGCTATAGAGGTAGTCCGTATACGTGGTCCGATTATCAGACCAAGGAGAGGCTCGATCGTAGCTTTTGGACGGATGCACTACGACGTATGTTTAGTCACTCTACCACTACGCATTTGCATCCCAGTTCTTCAGATCATAGTCCGTTATTAGTTGAGGTATGTGTCTCTCCTAACCTGCAAGCTGGCCGGAAGAAACGTTTCTTCCGGTTTGAACAGTTCTGGGCACAACATGCGGCATGTGAGGAGATCGTGAAGACTGGTTGGCAGGGAGATATAGTGGGCGATCCTATGCACAGGGTTTGTCAAAAAATACGAAATACCAGTACGACGTTGTATAATTGGCAACGTGATACTTTTCAGTTTCGCCAGACGGAGATGAGGGCAGTTAGGGCACGTTTGAATGAATTGTTGGAGGCACCATTTGACTCGGCCCTGAATCAAGAAAAACATGATCTGAATGTTCGCCTACAGGAGTTATTGACTCAAGAAGAAACTTATTGGAGTCAACGCTCCAAAGAGATGTGGTTGAAGTCGGGTGACAGCAACACCGCATACTTCCACCGTAGGGCCTCAAATCGAAGGCAGCGTAACTTGATTAAAGGTTTATTCGATGATGATGGAAGGTGGGTAACAGAGGCTGATCAAATTGAGCAGGTTGTTTGTAGTTTCTATCGTAACATGTTCTCAACAGAAGGTGTCAATGAAGTAGCTATGAATTTGATATTTGATACGGTGAGGCCAAAAGTAACACCGGAAATGAACATGGAATTGTTAGCTGAGTACACTGATGAGGAGATCCGAGCAGCGGCTTTCCATATGCATCCATCGAAGTCGCCTGGCCCTGATGGTATGTCACCATTCTTTTTTCAAAAGTATTGGAGCACTGTGGGCACTGACGTCTGTAATGCGGTACGATCTTTCTTCACCTCTGGCAATCTTACACGTGAGGTGAATTTCACCCACATAACTTTGATTCCAAAGGTACAGGAGCCGAAACTTATGACAGATTTACGTCCCATAGCATTGTGCAATGTTCTGTATAGGATTTGCTCGAAAGTTATGGCAAACAGATTAAAAGGTATGTTGGGAAAGGTGATTTCTCCATTACAAAGTGCCTTTGTGCCAGGACGATTGATTTCAGATAATACTTTAGTGGCAACGGAAGTGGCTCACTTTCTCCATAATGAACGAAGGGGGGCAACAAAGGAGCACTTCTCTTTGAAGCTGGACATTAGTAAGGCTTATGACAGGCTTGAGTGGAGTTTTCTTAGAGGTATCCTTTTAAAGCTGGGATTTGCTGAGGCATGGGTGGAGTTGGTAATGATCAGCATTCAAACAGTAAGCTATTCTTTTCTCTTAAATGGAGAAGTTCGGGGTTATGTGAAGCCAACTAGGGGAATAAGGCAAGGCGATCCTTTGTCTCCATATTTATTCATTTTGTGTGGAGAGGGTTTGACGTCACTAATGGAACATTTTGTGGACCGTCAATGGCTGCAAGGTCTTCAAATTAATTCTCAAGCGCCGGTGTTACACCATTTACTATTCGCGGATGATAGTTTTTTGTTTGGGACTGCAACTGAGGAAGAGTGTCAGCAGATCCGCAACATTCTGCATACATATGAGATTGCTTCAGGTCAAAGGGTAAATTTACAAAAGAGTGAAGTTGCTTTTAGTCGGGGAATGGACATGAGGAGGCAAGAGTTTCTTGCACACATGTTGGGAGTGAAAAGGGTCGACAAACATGATCGTTATCTGGGATTACCAACACATGTAGGTCTAAAAAAATCAGCTGCATTCAACTATTTGAAGGAGAAACTCACGAAGAAGGTGGTGAGCTGGAGAGCTAAGTTACTAAGTGGTGCAGGAAAGGAAATCTTAATAAAGGCGGTGGGGCAATCAGTCCCGATGTATGTGATGAATTGTTATAAGTTGCCTTTAGGTCTGTGCGACGATCTACATCAGTTATGTGCAGGTTTCTGGTGGGGCGATTTGGAAGAAAAGAACAAAATCCATTGGAGGTCATGGGACCGTCTGTGTATGTCTAAACAAGATGGTGGTATGGGGTTTAAAAACCTTCACTGGTTTAACATGGCTATGCTTGCGAAACAAAGTTGGAGATTACTCCAACATCCAGACTCTCTGATAGCGCAGCTGTACAAGGCTATCTATTTTTATAATTGTGATTTGGAGCAAGCTGAGGTAGGGAGCTCACCTTCCTTTTCTTGGCGTAGTATATGGGAAGCAAGGCAAGTCCTATTTCGAGGACTTCGGTGGCAGATCGGGGATGGAACTAAAGTGGAGATTTGGAAGCATAATTGGATTCCTGATGTGTATCCTCGCTGCCCTTCATCTCCACCTTCACATGATGCGCCCAGATTTGTAGCAGAGCTTATTGATCCTATTACTCGGATGTGGGATATAGACATGTTGCAGCGACATTTTAGTCCAGCGGATGTGGAGTTAATACTCACCATACCACTCAGCCAGGTTCCAAGAGATGATACACAGGTCTGGCATTTTAATAAGAAAGGTTTTTTTACAACTAACAGTGCTTATTATGTTGCGAAAGATTTAGCTTTGGGTTTGGTTCTTGCACCTAAACCCCCTATAGATCCTTTGAGGGTAGTTTGGAAGTCAATTTGGAGTGCAAACGTGCCTGGTAAAGTGGCTCTACATGCTTGGAGATTGTTCAGCAATATTCTGCCTACTCGGGTGAGTCTTAGCGCAAAAGGATATACAGGTCCATTGGACTGCAGCTTATGTGTGCATAATTACGAGGACAGTAGACATTTATTTATGGACTGCCCTCATGCTACTGAGATCTGGACTCAGGCTGGTGTGCGTTGGTCGGGCGGGAATGTTGATTCTATCCAAGCGTGGGTTATGGCTTTAATACCTACTTTATCCAAAGAAGAATTGAATAAGGTGCTGATGTTGTTGTGGGGTATATGGAAGAATAGAAATGCTCAAGTTTGGGAACAGAAAGGTAAGCATGCTTCTGACACATTGTTGTTGACGTTGGGATGGTATGAAGAGTACAAGAAGTATCATGTGGTTTCAAGAAGACAGAGGCCCCCACCTTCACATTGGATTGTTCCAGCTCCAGGCCAGTACAAAATGAATTGTGATGGAGCTTATCAAGCATCTTCCAGACGTGGTGGAGGAGGCTATGTCATGCGTGACAGTGCAGGCAGCTTTATAGCGGGACAGACGAAGCCATATGGCTTGCTTACATCACCTTTTCATGCTGAATTGCTAGCTCTTAGGGACGGTATATTGCTAGCTCGATCGCTGCATCACGAAGAGGTGGTGTTTGAGAGTGATTGCTCACTGCTAGTCCAAGCTATCCACTCTCCTTCGCAGGATCTAACCACTATGAATATACTAATTGAGGAAGTTAGACAACTGCTGCAAGATCACCGGGGATACAGTGTCCTGCATGTCCGGCGCGAAGCTAATATAGTTGCACATACCTTAGCGACCCATGCGCTAAGGTCTAGAGAAAGTCAAGTGTGGTTTGTACTAGCTCCCGAATTGATAAGGGATGCCATTCGAAACGATGTAATCGTTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

1556

Amino Acids

178.49

Weight (kDa)

8.76

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 197 - 411 2.7e-11 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 691 - 931 8.1e-26 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 1241 - 1307 7e-14 zinc-binding in reverse transcriptase
RVT_3 PF13456 1406 - 1528 6.2e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3021, 3270
AasI GACNNNNNNGTC 2 cut(s) 951, 3537
AatII GACGTC 2 cut(s) 1995, 2555
Acc36I ACCTGC 3 cut(s) 1278, 1738, 3056
AccB1I GGYRCC 1 cut(s) 1510
AccB7I CCANNNNNTGG 2 cut(s) 105, 786
AccBSI CCGCTC 2 cut(s) 525, 1042
AccI GTMKAC 4 cut(s) 312, 1115, 2829, 3880
AccII CGCG 2 cut(s) 2642, 4545
AclI AACGTT 1 cut(s) 1291
AcoI YGGCCR 2 cut(s) 948, 1279
AcsI RAATTY 4 cut(s) 398, 1810, 2047, 2740
AcuI CTGAAG 3 cut(s) 99, 1209, 2709
AcvI CACGTG 2 cut(s) 770, 2039
AcyI GRCGYC 3 cut(s) 257, 1992, 2552
AfaI GTAC 8 cut(s) 1420, 1877, 2007, 2079, 3251, 4133, 4208, 4617
AflIII ACRYGT 5 cut(s) 1773, 2036, 2808, 2863, 3519
AjiI CACGTC 1 cut(s) 4250
AjnI CCWGG 6 cut(s) 52, 1930, 2218, 3579, 3751, 4198
AjuI GAANNNNNNNTTGG 2 cut(s) 1487, 1519
Alw21I GWGCWC 4 cut(s) 534, 1979, 2307, 3305
Alw26I GTCTC 9 cut(s) 143, 248, 677, 704, 749, 917, 934, 1264, 2520
AlwNI CAGNNNCTG 1 cut(s) 1733
Ama87I CYCGRG 1 cut(s) 3804
AoxI GGCC 8 cut(s) 948, 1279, 1524, 1662, 1832, 1933, 4166, 4201
ApoI RAATTY 4 cut(s) 398, 1810, 2047, 2740
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AseI ATTAAT 1 cut(s) 2604
Asp700I GAANNNNTTC 1 cut(s) 1292
AspLEI GCGC 7 cut(s) 522, 2617, 3244, 3466, 3821, 4545, 4585
AspS9I GGNCC 8 cut(s) 1122, 1525, 1662, 1934, 2575, 3121, 3836, 4167
AsuC2I CCSGG 1 cut(s) 4517
AsuII TTCGAA 2 cut(s) 430, 4648
AsuNHI GCTAGC 2 cut(s) 4351, 4375
AvaI CYCGRG 1 cut(s) 3804
AvaII GGWCC 4 cut(s) 1122, 2575, 3121, 3836
BaeGI GKGCMC 3 cut(s) 1321, 1489, 1988
BaeI ACNNNNGTAYC 2 cut(s) 3588, 3621
BalI TGGCCA 1 cut(s) 950
BamHI GGATCC 1 cut(s) 666
BanI GGYRCC 1 cut(s) 1510
BanII GRGCYC 1 cut(s) 3305
BarI GAAGNNNNNNTAC 2 cut(s) 4075, 4107
BbrPI CACGTG 2 cut(s) 770, 2039
BbsI GAAGAC 5 cut(s) 351, 1042, 1355, 2588, 4165
Bbv12I GWGCWC 4 cut(s) 534, 1979, 2307, 3305
BbvCI CCTCAGC 2 cut(s) 2394, 3291
BceAI ACGGC 2 cut(s) 258, 570
BciT130I CCWGG 6 cut(s) 54, 1932, 2220, 3581, 3753, 4200
BciVI GTATCC 3 cut(s) 2381, 3438, 4514
BclI TGATCA 2 cut(s) 1735, 2418
BcnI CCSGG 1 cut(s) 4517
BcoDI GTCTC 9 cut(s) 143, 248, 677, 704, 749, 917, 934, 1264, 2520
BfmI CTRYAG 5 cut(s) 619, 1099, 1562, 3702, 3847
BfoI RGCGCY 2 cut(s) 523, 2618
BfuAI ACCTGC 3 cut(s) 1278, 1738, 3056
BfuI GTATCC 3 cut(s) 2381, 3438, 4514
BglII AGATCT 1 cut(s) 3915
BlpI GCTNAGC 1 cut(s) 699
Bme1390I CCNGG 7 cut(s) 54, 1932, 2220, 3581, 3753, 4200, 4517
Bme18I GGWCC 4 cut(s) 1122, 2575, 3121, 3836
BmeT110I CYCGRG 1 cut(s) 3804
BmgBI CACGTC 1 cut(s) 4250
BmgT120I GGNCC 8 cut(s) 1122, 1525, 1662, 1934, 2575, 3121, 3836, 4167
BmiI GGNNCC 6 cut(s) 668, 1512, 2085, 3122, 3585, 4169
BmrFI CCNGG 7 cut(s) 54, 1932, 2220, 3581, 3753, 4200, 4517
BmrI ACTGGG 2 cut(s) 918, 1212
BmtI GCTAGC 2 cut(s) 4355, 4379
BmuI ACTGGG 2 cut(s) 918, 1212
BpiI GAAGAC 5 cut(s) 351, 1042, 1355, 2588, 4165
BplI GAGNNNNNCTC 5 cut(s) 27, 509, 541, 2294, 2326
BpmI CTGGAG 4 cut(s) 1028, 1041, 2953, 4182
Bpu10I CCTNAGC 4 cut(s) 2394, 3291, 4570, 4586
Bpu1102I GCTNAGC 1 cut(s) 699
Bpu14I TTCGAA 2 cut(s) 430, 4648
BpuEI CTTGAG 4 cut(s) 1563, 2369, 2595, 4047
BpuMI CCSGG 1 cut(s) 4517
Bsa29I ATCGAT 2 cut(s) 845, 1089
BsaAI YACGTR 3 cut(s) 770, 1119, 2039
BsaBI GATNNNNATC 3 cut(s) 2844, 3051, 3426
BsaHI GRCGYC 3 cut(s) 257, 1992, 2552
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 4 cut(s) 662, 968, 1137, 4516
BsaWI WCCGGW 2 cut(s) 1299, 1846
BsaXI ACNNNNNCTCC 6 cut(s) 282, 312, 1356, 1386, 4251, 4281
Bse118I RCCGGY 1 cut(s) 2617
Bse1I ACTGG 9 cut(s) 913, 951, 951, 1024, 1218, 1357, 1416, 3179, 4204
Bse3DI GCAATG 1 cut(s) 2131
Bse8I GATNNNNATC 3 cut(s) 2844, 3051, 3426
BseBI CCWGG 6 cut(s) 54, 1932, 2220, 3581, 3753, 4200
BseCI ATCGAT 2 cut(s) 845, 1089
BseDI CCNNGG 4 cut(s) 662, 968, 1137, 4516
BseJI GATNNNNATC 3 cut(s) 2844, 3051, 3426
BseMI GCAATG 1 cut(s) 2131
BseNI ACTGG 9 cut(s) 913, 951, 951, 1024, 1218, 1357, 1416, 3179, 4204
BseRI GAGGAG 8 cut(s) 383, 884, 1038, 1041, 1352, 1901, 2800, 4272
BseSI GKGCMC 3 cut(s) 1321, 1489, 1988
BseYI CCCAGC 1 cut(s) 2383
BsgI GTGCAG 4 cut(s) 402, 2976, 3084, 4302
Bsh1236I CGCG 2 cut(s) 2642, 4545
Bsh1285I CGRYCG 2 cut(s) 1153, 4385
BshFI GGCC 8 cut(s) 950, 1281, 1526, 1664, 1834, 1935, 4168, 4203
BshNI GGYRCC 1 cut(s) 1510
BshVI ATCGAT 2 cut(s) 845, 1089
BsiEI CGRYCG 2 cut(s) 1153, 4385
BsiHKAI GWGCWC 4 cut(s) 534, 1979, 2307, 3305
BsiHKCI CYCGRG 1 cut(s) 3804
BsiSI CCGG 6 cut(s) 1282, 1300, 1847, 2618, 4516, 4540
BslFI GGGAC 6 cut(s) 2094, 2677, 2981, 3134, 4314, 4376
BsmAI GTCTC 9 cut(s) 143, 248, 677, 704, 749, 917, 934, 1264, 2520
BsmBI CGTCTC 2 cut(s) 248, 934
BsmFI GGGAC 6 cut(s) 2094, 2677, 2981, 3134, 4314, 4376
BsmI GAATGC 2 cut(s) 2424, 2888
BsnI GGCC 8 cut(s) 950, 1281, 1526, 1664, 1834, 1935, 4168, 4203
Bso31I GGTCTC 1 cut(s) 677
BsoBI CYCGRG 1 cut(s) 3804
Bsp119I TTCGAA 2 cut(s) 430, 4648
Bsp1286I GDGCHC 7 cut(s) 534, 1321, 1489, 1979, 1988, 2307, 3305
Bsp1407I TGTACA 1 cut(s) 3249
Bsp1720I GCTNAGC 1 cut(s) 699
Bsp19I CCATGG 1 cut(s) 968
BspANI GGCC 8 cut(s) 950, 1281, 1526, 1664, 1834, 1935, 4168, 4203
BspDI ATCGAT 2 cut(s) 845, 1089
BspFNI CGCG 2 cut(s) 2642, 4545
BspLI GGNNCC 6 cut(s) 668, 1512, 2085, 3122, 3585, 4169
BspMAI CTGCAG 1 cut(s) 3851
BspMI ACCTGC 3 cut(s) 1278, 1738, 3056
BspOI GCTAGC 2 cut(s) 4355, 4379
BspT104I TTCGAA 2 cut(s) 430, 4648
BspT107I GGYRCC 1 cut(s) 1510
BspTNI GGTCTC 1 cut(s) 677
BsrBI CCGCTC 2 cut(s) 525, 1042
BsrDI GCAATG 1 cut(s) 2131
BsrFI RCCGGY 1 cut(s) 2617
BsrGI TGTACA 1 cut(s) 3249
BsrI ACTGG 9 cut(s) 913, 951, 951, 1024, 1218, 1357, 1416, 3179, 4204
BssAI RCCGGY 1 cut(s) 2617
BssECI CCNNGG 4 cut(s) 662, 968, 1137, 4516
BssNAI GTATAC 2 cut(s) 313, 1116
BssNI GRCGYC 3 cut(s) 257, 1992, 2552
BssT1I CCWWGG 2 cut(s) 968, 1137
Bst1107I GTATAC 2 cut(s) 313, 1116
Bst2UI CCWGG 6 cut(s) 54, 1932, 2220, 3581, 3753, 4200
Bst6I CTCTTC 4 cut(s) 913, 2673, 4122, 4392
BstACI GRCGYC 3 cut(s) 257, 1992, 2552
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstAUI TGTACA 1 cut(s) 3249
BstBAI YACGTR 3 cut(s) 770, 1119, 2039
BstBI TTCGAA 2 cut(s) 430, 4648
BstDSI CCRYGG 1 cut(s) 968
BstFNI CGCG 2 cut(s) 2642, 4545
BstH2I RGCGCY 2 cut(s) 523, 2618
BstHHI GCGC 7 cut(s) 522, 2617, 3244, 3466, 3821, 4545, 4585
BstMAI GTCTC 9 cut(s) 143, 248, 677, 704, 749, 917, 934, 1264, 2520
BstMCI CGRYCG 2 cut(s) 1153, 4385
BstNI CCWGG 6 cut(s) 54, 1932, 2220, 3581, 3753, 4200
BstSCI CCNGG 7 cut(s) 52, 1930, 2218, 3579, 3751, 4198, 4515
BstSFI CTRYAG 5 cut(s) 619, 1099, 1562, 3702, 3847
BstSLI GKGCMC 3 cut(s) 1321, 1489, 1988
BstUI CGCG 2 cut(s) 2642, 4545
BstV2I GAAGAC 5 cut(s) 351, 1042, 1355, 2588, 4165
BstX2I RGATCY 7 cut(s) 666, 996, 1890, 2691, 3706, 3915, 4459
BstYI RGATCY 7 cut(s) 666, 996, 1890, 2691, 3706, 3915, 4459
BstZ17I GTATAC 2 cut(s) 313, 1116
Bsu15I ATCGAT 2 cut(s) 845, 1089
BsuI GTATCC 3 cut(s) 2381, 3438, 4514
BsuRI GGCC 8 cut(s) 950, 1281, 1526, 1664, 1834, 1935, 4168, 4203
BsuTUI ATCGAT 2 cut(s) 845, 1089
BtgI CCRYGG 1 cut(s) 968
BtrI CACGTC 1 cut(s) 4250
BtsI GCAGTG 1 cut(s) 4424
BtsIMutI CAGTG 9 cut(s) 342, 1878, 1977, 1986, 3172, 3649, 4285, 4424, 4531
BveI ACCTGC 3 cut(s) 1278, 1738, 3056
CaiI CAGNNNCTG 1 cut(s) 1733
CfoI GCGC 7 cut(s) 522, 2617, 3244, 3466, 3821, 4545, 4585
Cfr10I RCCGGY 1 cut(s) 2617
Cfr13I GGNCC 8 cut(s) 1122, 1525, 1662, 1934, 2575, 3121, 3836, 4167
ClaI ATCGAT 2 cut(s) 845, 1089
CseI GACGC 1 cut(s) 265
Csp6I GTAC 8 cut(s) 1419, 1876, 2006, 2078, 3250, 4132, 4207, 4616
CspCI CAANNNNNGTGG 2 cut(s) 2241, 2276
CviQI GTAC 8 cut(s) 1419, 1876, 2006, 2078, 3250, 4132, 4207, 4616
DraI TTTAAA 2 cut(s) 2379, 3163
DrdI GACNNNNNNGTC 2 cut(s) 951, 3537
DseDI GACNNNNNNGTC 2 cut(s) 951, 3537
EaeI YGGCCR 2 cut(s) 948, 1279
Eam1104I CTCTTC 4 cut(s) 913, 2673, 4122, 4392
EarI CTCTTC 4 cut(s) 913, 2673, 4122, 4392
EciI GGCGGA 1 cut(s) 881
Ecl136II GAGCTC 1 cut(s) 3303
Eco130I CCWWGG 2 cut(s) 968, 1137
Eco24I GRGCYC 1 cut(s) 3305
Eco31I GGTCTC 1 cut(s) 677
Eco47I GGWCC 4 cut(s) 1122, 2575, 3121, 3836
Eco53kI GAGCTC 1 cut(s) 3303
Eco57I CTGAAG 3 cut(s) 99, 1209, 2709
Eco72I CACGTG 2 cut(s) 770, 2039
Eco88I CYCGRG 1 cut(s) 3804
EcoICRI GAGCTC 1 cut(s) 3303
EcoO109I RGGNCCY 2 cut(s) 1662, 4167
EcoRII CCWGG 6 cut(s) 52, 1930, 2218, 3579, 3751, 4198
EcoT14I CCWWGG 2 cut(s) 968, 1137
EcoT22I ATGCAT 1 cut(s) 1917
EcoT38I GRGCYC 1 cut(s) 3305
ErhI CCWWGG 2 cut(s) 968, 1137
Esp3I CGTCTC 2 cut(s) 248, 934
FaqI GGGAC 6 cut(s) 2094, 2677, 2981, 3134, 4314, 4376
FauI CCCGC 4 cut(s) 550, 974, 3941, 4291
FauNDI CATATG 3 cut(s) 1911, 2713, 4315
FbaI TGATCA 2 cut(s) 1735, 2418
FblI GTMKAC 4 cut(s) 312, 1115, 2829, 3880
FriOI GRGCYC 1 cut(s) 3305
GlaI GCGC 7 cut(s) 521, 2616, 3243, 3465, 3820, 4544, 4584
GsaI CCCAGC 1 cut(s) 2387
GsuI CTGGAG 4 cut(s) 1028, 1041, 2953, 4182
HaeII RGCGCY 2 cut(s) 523, 2618
HaeIII GGCC 8 cut(s) 950, 1281, 1526, 1664, 1834, 1935, 4168, 4203
HapII CCGG 6 cut(s) 1282, 1300, 1847, 2618, 4516, 4540
HgaI GACGC 1 cut(s) 265
HhaI GCGC 7 cut(s) 522, 2617, 3244, 3466, 3821, 4545, 4585
Hin1I GRCGYC 3 cut(s) 257, 1992, 2552
Hin6I GCGC 7 cut(s) 520, 2615, 3242, 3464, 3819, 4543, 4583
HinP1I GCGC 7 cut(s) 520, 2615, 3242, 3464, 3819, 4543, 4583
HincII GTYRAC 3 cut(s) 1601, 2830, 4110
HindII GTYRAC 3 cut(s) 1601, 2830, 4110
HindIII AAGCTT 1 cut(s) 1053
HpaII CCGG 6 cut(s) 1282, 1300, 1847, 2618, 4516, 4540
Hpy166II GTNNAC 9 cut(s) 313, 340, 1116, 1601, 1878, 2575, 2830, 3881, 4110
Hpy8I GTNNAC 9 cut(s) 313, 340, 1116, 1601, 1878, 2575, 2830, 3881, 4110
Hpy99I CGWCG 4 cut(s) 829, 1182, 1426, 3047
Hsp92I GRCGYC 3 cut(s) 257, 1992, 2552
HspAI GCGC 7 cut(s) 520, 2615, 3242, 3464, 3819, 4543, 4583
Ksp22I TGATCA 2 cut(s) 1735, 2418
MbiI CCGCTC 2 cut(s) 525, 1042
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 7 cut(s) 666, 996, 1890, 2691, 3706, 3915, 4459
MhlI GDGCHC 7 cut(s) 534, 1321, 1489, 1979, 1988, 2307, 3305
MlsI TGGCCA 1 cut(s) 950
MluNI TGGCCA 1 cut(s) 950
MlyI GAGTC 8 cut(s) 365, 783, 1514, 1570, 1606, 3224, 3820, 3916
MmeI TCCRAC 4 cut(s) 268, 1485, 3188, 3244
Mox20I TGGCCA 1 cut(s) 950
Mph1103I ATGCAT 1 cut(s) 1917
MroXI GAANNNNTTC 1 cut(s) 1292
MscI TGGCCA 1 cut(s) 950
MslI CAYNNNNRTG 4 cut(s) 35, 837, 2712, 3188
Msp20I TGGCCA 1 cut(s) 950
MspA1I CMGCKG 5 cut(s) 543, 1901, 2885, 3247, 3545
MspI CCGG 6 cut(s) 1282, 1300, 1847, 2618, 4516, 4540
MspR9I CCNGG 7 cut(s) 54, 1932, 2220, 3581, 3753, 4200, 4517
MunI CAATTG 1 cut(s) 456
Mva1269I GAATGC 2 cut(s) 2424, 2888
MvaI CCWGG 6 cut(s) 54, 1932, 2220, 3581, 3753, 4200
MvnI CGCG 2 cut(s) 2642, 4545
NciI CCSGG 1 cut(s) 4517
NcoI CCATGG 1 cut(s) 968
NdeI CATATG 3 cut(s) 1911, 2713, 4315
NheI GCTAGC 2 cut(s) 4351, 4375
NlaIV GGNNCC 6 cut(s) 668, 1512, 2085, 3122, 3585, 4169
NmeAIII GCCGAG 1 cut(s) 1502
NmuCI GTSAC 5 cut(s) 1190, 1631, 1947, 2553, 4274
NsiI ATGCAT 1 cut(s) 1917
NspV TTCGAA 2 cut(s) 430, 4648
PaeI GCATGC 1 cut(s) 4091
PciI ACATGT 4 cut(s) 1773, 2808, 2863, 3519
PcsI WCGNNNNNNNCGW 2 cut(s) 1678, 4659
PctI GAATGC 2 cut(s) 2424, 2888
PdmI GAANNNNTTC 1 cut(s) 1292
PfeI GAWTC 5 cut(s) 764, 1531, 2068, 3415, 3959
PflMI CCANNNNNTGG 2 cut(s) 105, 786
PfoI TCCNGGA 1 cut(s) 52
Ple19I CGATCG 2 cut(s) 1153, 4385
PleI GAGTC 8 cut(s) 364, 782, 1514, 1570, 1605, 3224, 3819, 3916
PmaCI CACGTG 2 cut(s) 770, 2039
PmlI CACGTG 2 cut(s) 770, 2039
PpsI GAGTC 8 cut(s) 364, 782, 1514, 1570, 1605, 3224, 3819, 3916
Ppu21I YACGTR 3 cut(s) 770, 1119, 2039
PscI ACATGT 4 cut(s) 1773, 2808, 2863, 3519
PshBI ATTAAT 1 cut(s) 2604
PsiI TTATAA 2 cut(s) 3021, 3270
Psp124BI GAGCTC 1 cut(s) 3305
Psp1406I AACGTT 1 cut(s) 1291
Psp6I CCWGG 6 cut(s) 52, 1930, 2218, 3579, 3751, 4198
PspCI CACGTG 2 cut(s) 770, 2039
PspFI CCCAGC 1 cut(s) 2383
PspGI CCWGG 6 cut(s) 52, 1930, 2218, 3579, 3751, 4198
PspN4I GGNNCC 6 cut(s) 668, 1512, 2085, 3122, 3585, 4169
PspPI GGNCC 8 cut(s) 1122, 1525, 1662, 1934, 2575, 3121, 3836, 4167
PstI CTGCAG 1 cut(s) 3851
PstNI CAGNNNCTG 1 cut(s) 1733
PsuI RGATCY 7 cut(s) 666, 996, 1890, 2691, 3706, 3915, 4459
PvuI CGATCG 2 cut(s) 1153, 4385
PvuII CAGCTG 2 cut(s) 2885, 3247
RsaI GTAC 8 cut(s) 1420, 1877, 2007, 2079, 3251, 4133, 4208, 4617
RsaNI GTAC 8 cut(s) 1419, 1876, 2006, 2078, 3250, 4132, 4207, 4616
RseI CAYNNNNRTG 4 cut(s) 35, 837, 2712, 3188
SacI GAGCTC 1 cut(s) 3305
SalI GTCGAC 1 cut(s) 2828
Sau96I GGNCC 8 cut(s) 1122, 1525, 1662, 1934, 2575, 3121, 3836, 4167
SchI GAGTC 8 cut(s) 365, 783, 1514, 1570, 1606, 3224, 3820, 3916
ScrFI CCNGG 7 cut(s) 54, 1932, 2220, 3581, 3753, 4200, 4517
SduI GDGCHC 7 cut(s) 534, 1321, 1489, 1979, 1988, 2307, 3305
SfcI CTRYAG 5 cut(s) 619, 1099, 1562, 3702, 3847
SfuI TTCGAA 2 cut(s) 430, 4648
SgrAI CRCCGGYG 1 cut(s) 2617
SinI GGWCC 4 cut(s) 1122, 2575, 3121, 3836
SmiMI CAYNNNNRTG 4 cut(s) 35, 837, 2712, 3188
SmlI CTYRAG 4 cut(s) 1578, 2348, 2610, 4062
SmoI CTYRAG 4 cut(s) 1578, 2348, 2610, 4062
SphI GCATGC 1 cut(s) 4091
SspI AATATT 1 cut(s) 3793
SstI GAGCTC 1 cut(s) 3305
StyD4I CCNGG 7 cut(s) 52, 1930, 2218, 3579, 3751, 4198, 4515
StyI CCWWGG 2 cut(s) 968, 1137
TatI WGTACW 5 cut(s) 1875, 3249, 4131, 4206, 4615
TauI GCSGC 4 cut(s) 525, 546, 1331, 1904
TfiI GAWTC 5 cut(s) 764, 1531, 2068, 3415, 3959
TscAI CASTG 9 cut(s) 342, 1885, 1984, 1993, 3179, 3649, 4285, 4431, 4531
TseFI GTSAC 5 cut(s) 1190, 1631, 1947, 2553, 4274
Tsp45I GTSAC 5 cut(s) 1190, 1631, 1947, 2553, 4274
TspGWI ACGGA 6 cut(s) 803, 1101, 1181, 1227, 1481, 2270
TspRI CASTG 9 cut(s) 342, 1885, 1984, 1993, 3179, 3649, 4285, 4431, 4531
Van91I CCANNNNNTGG 2 cut(s) 105, 786
VpaK11BI GGWCC 4 cut(s) 1122, 2575, 3121, 3836
VspI ATTAAT 1 cut(s) 2604
XapI RAATTY 4 cut(s) 398, 1810, 2047, 2740
XbaI TCTAGA 1 cut(s) 4592
XcmI CCANNNNNNNNNTGG 1 cut(s) 4179
XmiI GTMKAC 4 cut(s) 312, 1115, 2829, 3880
XmnI GAANNNNTTC 1 cut(s) 1292
ZraI GACGTC 2 cut(s) 1993, 2553
Zsp2I ATGCAT 1 cut(s) 1917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.