RchiOBHm_Chr6g0284091

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015193
Physical Location & Seq
Forward (+)
47384016 .. 47385711
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25477

Sequence Viewer

Length: 594 bp
ATGGCTTCCGACGACCCCAGAGCTTCAGACGCAGAATCAGAGGCTCCTCCATCCTCGTCTTGGAAGGACCGCATCGTCGTCCCCACACTCGTTGCCGGAATCGTTGGCGCCGGAGCTGGGTTGGTCTCCAAGCATCGGAAAGTCGTCGGCCTCCCAACCAGCTCCGCCACTTACGCTGCTAATTTCGCCATTGTCACAGCTTGTTACTGCGGGGCGCGTGAGTATGTAGGAATAACTAGAAAAACAGGGCCTGATGATCTGCTCAACTCTGCAATTGCCGGTTTTGGCAGTGGGGCTATTCTTGGGCGGCTTTACGGTGGTCCAGTTGCTGCTGTCCGCTACTCAATACTCTTTACTATTGCTGGGACAACATTGGACTATGGTACAGTTAAACTACGACCTGTCTTGAAGAGCTACAAAGAATCTATGCTTGGGAGTAATGATGATTCAAAGAAGAATGATGGCTGGCTGAAACTGCCTGACTGGTCCCCTATCAAAGTACTTGATGAAGAAGCCCTTGCTGCAAAGCAGGCTCGAGAAAAGCAGTTATATGCACAGAGGGGACTTGGGAACTTCACCAAAGAAGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

197

Amino Acids

20.87

Weight (kDa)

8.78

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 50 - 120 1.1e-06 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 107
AccII CGCG 1 cut(s) 217
AciI CCGC 5 cut(s) 70, 165, 210, 307, 337
AcuI CTGAAG 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 2 cut(s) 385, 501
AfiI CCNNNNNNNGG 3 cut(s) 60, 117, 135
AgsI TTSAA 2 cut(s) 409, 450
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AluBI AGCT 5 cut(s) 23, 116, 162, 200, 414
AluI AGCT 5 cut(s) 23, 116, 162, 200, 414
Alw26I GTCTC 1 cut(s) 130
AlwNI CAGNNNCTG 2 cut(s) 251, 329
Ama87I CYCGRG 1 cut(s) 534
AoxI GGCC 2 cut(s) 148, 248
ApeKI GCWGC 3 cut(s) 176, 329, 521
AspLEI GCGC 2 cut(s) 110, 217
AspS9I GGNCC 4 cut(s) 67, 248, 320, 486
AsuHPI GGTGA 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 534
AvaII GGWCC 3 cut(s) 67, 320, 486
BanI GGYRCC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 163, 316, 508
BccI CCATC 2 cut(s) 58, 455
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 1 cut(s) 237
BfoI RGCGCY 1 cut(s) 111
BisI GCNGC 4 cut(s) 177, 308, 330, 522
BlsI GCNGC 4 cut(s) 178, 309, 331, 523
BmcAI AGTACT 1 cut(s) 501
Bme18I GGWCC 3 cut(s) 67, 320, 486
BmeT110I CYCGRG 1 cut(s) 534
BmgT120I GGNCC 4 cut(s) 67, 248, 320, 486
BmiI GGNNCC 3 cut(s) 45, 109, 488
BmsI GCATC 2 cut(s) 81, 142
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 130
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 117, 135
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 323, 488
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 3 cut(s) 60, 117, 135
BseNI ACTGG 2 cut(s) 323, 488
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 3 cut(s) 163, 316, 508
BseYI CCCAGC 2 cut(s) 116, 362
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 150, 250
BshNI GGYRCC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 534
BsiSI CCGG 3 cut(s) 96, 111, 279
BslFI GGGAC 4 cut(s) 65, 379, 472, 576
BslI CCNNNNNNNGG 3 cut(s) 60, 117, 135
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 4 cut(s) 65, 379, 472, 576
BsnI GGCC 2 cut(s) 150, 250
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 534
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 5 cut(s) 70, 165, 210, 307, 337
BspANI GGCC 2 cut(s) 150, 250
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 3 cut(s) 45, 109, 488
BspQI GCTCTTC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 323, 488
BssAI RCCGGY 1 cut(s) 278
BssMI GATC 1 cut(s) 256
BssNI GRCGYC 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 317, 388
Bst6I CTCTTC 2 cut(s) 404, 579
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 2 cut(s) 467, 531
BstF5I GGATG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 217
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 6 cut(s) 29, 173, 185, 475, 521, 530
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 3 cut(s) 163, 316, 508
BsuRI GGCC 2 cut(s) 150, 250
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 467, 531
CaiI CAGNNNCTG 2 cut(s) 251, 329
CfoI GCGC 2 cut(s) 110, 217
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 4 cut(s) 67, 248, 320, 486
CseI GACGC 1 cut(s) 38
Csp6I GTAC 2 cut(s) 384, 500
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviQI GTAC 2 cut(s) 384, 500
DinI GGCGCC 1 cut(s) 109
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 404, 579
EarI CTCTTC 2 cut(s) 404, 579
EciI GGCGGA 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 3 cut(s) 67, 320, 486
Eco57I CTGAAG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 248
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
FaiI YATR 5 cut(s) 225, 381, 428, 550, 552
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FaqI GGGAC 4 cut(s) 65, 379, 472, 576
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 4 cut(s) 177, 308, 330, 522
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 4 cut(s) 177, 308, 330, 522
FspBI CTAG 1 cut(s) 237
GlaI GCGC 2 cut(s) 109, 216
GluI GCNGC 4 cut(s) 177, 308, 330, 522
GsaI CCCAGC 2 cut(s) 120, 366
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 2 cut(s) 150, 250
HapII CCGG 3 cut(s) 96, 111, 279
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 110, 217
Hin1I GRCGYC 1 cut(s) 108
Hin6I GCGC 2 cut(s) 108, 215
HinP1I GCGC 2 cut(s) 108, 215
HinfI GANTC 5 cut(s) 35, 99, 422, 446, 587
HpaII CCGG 3 cut(s) 96, 111, 279
HphI GGTGA 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 10, 28, 40, 138
Hpy188III TCNNGA 2 cut(s) 406, 536
Hpy99I CGWCG 3 cut(s) 14, 80, 149
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 317, 388
HpyCH4V TGCA 3 cut(s) 272, 524, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 173, 185, 475, 521, 530
Hsp92I GRCGYC 1 cut(s) 108
HspAI GCGC 2 cut(s) 108, 215
KasI GGCGCC 1 cut(s) 107
Kzo9I GATC 1 cut(s) 256
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 3 cut(s) 49, 113, 167
Lsp1109I GCAGC 3 cut(s) 163, 316, 508
LweI GCATC 2 cut(s) 81, 142
MaeI CTAG 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 193, 203
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 3 cut(s) 421, 466, 521
MfeI CAATTG 1 cut(s) 273
MluCI AATT 2 cut(s) 181, 273
Mly113I GGCGCC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 5 cut(s) 34, 57, 64, 161, 552
MseI TTAA 2 cut(s) 390, 592
MspI CCGG 3 cut(s) 96, 111, 279
MunI CAATTG 1 cut(s) 273
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 6 cut(s) 29, 173, 185, 475, 521, 530
NarI GGCGCC 1 cut(s) 108
NdeII GATC 1 cut(s) 256
NlaIV GGNNCC 3 cut(s) 45, 109, 488
NmuCI GTSAC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 404
PfeI GAWTC 4 cut(s) 35, 99, 422, 446
PkrI GCNGC 4 cut(s) 178, 309, 331, 523
PluTI GGCGCC 1 cut(s) 111
PspFI CCCAGC 2 cut(s) 116, 362
PspN4I GGNNCC 3 cut(s) 45, 109, 488
PspPI GGNCC 4 cut(s) 67, 248, 320, 486
PstNI CAGNNNCTG 2 cut(s) 251, 329
RsaI GTAC 2 cut(s) 385, 501
RsaNI GTAC 2 cut(s) 384, 500
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 2 cut(s) 390, 592
SatI GCNGC 4 cut(s) 177, 308, 330, 522
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 4 cut(s) 67, 248, 320, 486
ScaI AGTACT 1 cut(s) 501
SetI ASST 6 cut(s) 25, 118, 164, 202, 403, 416
SfaNI GCATC 2 cut(s) 81, 142
SfoI GGCGCC 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 534
SinI GGWCC 3 cut(s) 67, 320, 486
SlaI CTCGAG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
Sse9I AATT 2 cut(s) 181, 273
SsiI CCGC 5 cut(s) 70, 165, 210, 307, 337
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 237
TaaI ACNGT 2 cut(s) 317, 388
TaqI TCGA 1 cut(s) 535
TasI AATT 2 cut(s) 181, 273
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 4 cut(s) 35, 99, 422, 446
Tru1I TTAA 2 cut(s) 390, 592
Tru9I TTAA 2 cut(s) 390, 592
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 3 cut(s) 176, 329, 521
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 522
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 3 cut(s) 67, 320, 486
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XhoI CTCGAG 1 cut(s) 534
XspI CTAG 1 cut(s) 237
ZrmI AGTACT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.