pycom05g15820

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015193
Physical Location & Seq
Forward (+)
19315977 .. 19317684
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15820.4

Sequence Viewer

Length: 684 bp
ATGTTTTCAGTTTTCATTTTTTTTTTCACCCAAAATTATAAAAATAAGCTCTCAGGCGAAGAGCAAAAGCAAATTTCACATCTTTTATTACAATCATGGCTTCCGATCGATCCCAAAGCCATAGAAGCAGAGCCACCGCCGTCGTCTTGGAAGGAGCGCATCGTCTTTCCCACTCTCCTAGCTGGGATTCTCGGCGGAGGAGCTGGGTTGGTCTCCAAGCATCGAAAAGTCGTGGGTCTCCCGACCAGTTGCGCCACCTATGCTGCCAATTTCGCCATTGTTACCGCTTGTTACTGCGGGGCTCGTGAATATGTGGGAGTAACTCGAAAAACTGGACCTGATGATCTTGTAAACTCTGCAATTGCTGGGTTTGGCACTGGTGCTATTCTTGGGCGTCTTCAGGGTGGTCGAATTGGTGCTGTACGGTACTCAATCATCTTTACTCTTGTTGGGACAACTGTGGATTATGCTACGATTAGACTAAGACCTGTGTTAAAGAGCTACAAAGAATCTATGCTTGGGAGTAATGACGGGTCACAGAAGAATGACGGTTGGTTGAAAATGCCCGACTGGTCTCCTATCAAAGTACTTGACGAGGAAGCCCTCGCTGCAAAACAGGCTCGTGAACAAAAGATGTATGCACAGAGGGCAGCACTTGGTAACCTAAGCAAAAAAGAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

228

Amino Acids

24.73

Weight (kDa)

9.47

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 39
AciI CCGC 4 cut(s) 137, 195, 285, 297
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 394
AfaI GTAC 3 cut(s) 423, 428, 588
AgsI TTSAA 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 501, 533
AluBI AGCT 4 cut(s) 49, 182, 203, 501
AluI AGCT 4 cut(s) 49, 182, 203, 501
Alw26I GTCTC 3 cut(s) 217, 242, 579
AlwI GGATC 1 cut(s) 104
ApeKI GCWGC 3 cut(s) 263, 608, 650
ApoI RAATTY 1 cut(s) 72
AspLEI GCGC 2 cut(s) 159, 254
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 304
BauI CACGAG 2 cut(s) 303, 621
BbsI GAAGAC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 250, 595, 662
BceAI ACGGC 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 217, 242, 579
BfaI CTAG 1 cut(s) 179
BisI GCNGC 3 cut(s) 264, 609, 651
BlsI GCNGC 3 cut(s) 265, 610, 652
BmcAI AGTACT 1 cut(s) 588
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 335
BmsI GCATC 2 cut(s) 168, 229
BpiI GAAGAC 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 665
Bsa29I ATCGAT 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 394
BsaI GGTCTC 3 cut(s) 217, 242, 579
Bse1I ACTGG 4 cut(s) 246, 337, 382, 575
BseCI ATCGAT 1 cut(s) 108
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 4 cut(s) 246, 337, 382, 575
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 3 cut(s) 250, 595, 662
BseYI CCCAGC 3 cut(s) 182, 203, 365
Bsh1285I CGRYCG 1 cut(s) 108
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 466
BsmAI GTCTC 3 cut(s) 217, 242, 579
BsmFI GGGAC 1 cut(s) 466
Bso31I GGTCTC 3 cut(s) 217, 242, 579
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 3 cut(s) 105, 109, 343
BspACI CCGC 4 cut(s) 137, 195, 285, 297
BspCNI CTCAG 1 cut(s) 65
BspDI ATCGAT 1 cut(s) 108
BspPI GGATC 1 cut(s) 104
BspQI GCTCTTC 1 cut(s) 54
BspTNI GGTCTC 3 cut(s) 217, 242, 579
BsrI ACTGG 4 cut(s) 246, 337, 382, 575
BssMI GATC 3 cut(s) 105, 109, 343
BssNI GRCGYC 1 cut(s) 394
BssSI CACGAG 2 cut(s) 303, 621
Bst2BI CACGAG 2 cut(s) 303, 621
Bst4CI ACNGT 3 cut(s) 426, 460, 551
Bst6I CTCTTC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 52, 482, 665
BstEII GGTNACC 1 cut(s) 659
BstHHI GCGC 2 cut(s) 159, 254
BstKTI GATC 3 cut(s) 108, 112, 346
BstMAI GTCTC 3 cut(s) 217, 242, 579
BstMBI GATC 3 cut(s) 105, 109, 343
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 6 cut(s) 125, 260, 272, 608, 617, 647
BstPI GGTNACC 1 cut(s) 659
BstV1I GCAGC 3 cut(s) 250, 595, 662
BstV2I GAAGAC 1 cut(s) 389
Bsu15I ATCGAT 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 375
CfoI GCGC 2 cut(s) 159, 254
Cfr13I GGNCC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 108
CseI GACGC 1 cut(s) 383
Csp6I GTAC 3 cut(s) 422, 427, 587
CviAII CATG 1 cut(s) 96
CviQI GTAC 3 cut(s) 422, 427, 587
DdeI CTNAG 3 cut(s) 52, 482, 665
DpnI GATC 3 cut(s) 107, 111, 345
DpnII GATC 3 cut(s) 105, 109, 343
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
EciI GGCGGA 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 304
Eco31I GGTCTC 3 cut(s) 217, 242, 579
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 659
EcoO65I GGTNACC 1 cut(s) 659
EcoT38I GRGCYC 1 cut(s) 304
FaeI CATG 1 cut(s) 99
FaiI YATR 8 cut(s) 39, 97, 122, 261, 312, 468, 515, 639
FaqI GGGAC 1 cut(s) 466
FatI CATG 1 cut(s) 95
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 3 cut(s) 264, 609, 651
FriOI GRGCYC 1 cut(s) 304
Fsp4HI GCNGC 3 cut(s) 264, 609, 651
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 158, 253
GluI GCNGC 3 cut(s) 264, 609, 651
GsaI CCCAGC 3 cut(s) 186, 207, 369
HgaI GACGC 1 cut(s) 383
HhaI GCGC 2 cut(s) 159, 254
Hin1I GRCGYC 1 cut(s) 394
Hin1II CATG 1 cut(s) 99
Hin6I GCGC 2 cut(s) 157, 252
HinP1I GCGC 2 cut(s) 157, 252
HinfI GANTC 3 cut(s) 187, 509, 677
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 352, 626
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 3 cut(s) 241, 305, 623
Hpy8I GTNNAC 2 cut(s) 352, 626
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 3 cut(s) 426, 460, 551
HpyCH4V TGCA 3 cut(s) 359, 611, 641
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 260, 272, 608, 617, 647
HpyF3I CTNAG 3 cut(s) 52, 482, 665
Hsp92I GRCGYC 1 cut(s) 394
Hsp92II CATG 1 cut(s) 99
HspAI GCGC 2 cut(s) 157, 252
Kzo9I GATC 3 cut(s) 105, 109, 343
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 154, 200
Lsp1109I GCAGC 3 cut(s) 250, 595, 662
LweI GCATC 2 cut(s) 168, 229
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 5 cut(s) 280, 290, 319, 534, 659
MalI GATC 3 cut(s) 107, 111, 345
MboI GATC 3 cut(s) 105, 109, 343
MboII GAAGA 3 cut(s) 71, 389, 553
MfeI CAATTG 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 5 cut(s) 34, 72, 268, 360, 411
MnlI CCTC 4 cut(s) 191, 589, 614, 639
MseI TTAA 1 cut(s) 494
MunI CAATTG 1 cut(s) 360
MwoI GCNNNNNNNGC 6 cut(s) 125, 260, 272, 608, 617, 647
NdeII GATC 3 cut(s) 105, 109, 343
NlaIII CATG 1 cut(s) 99
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 187, 509
PkrI GCNGC 3 cut(s) 265, 610, 652
Ple19I CGATCG 1 cut(s) 108
PsiI TTATAA 1 cut(s) 39
PspEI GGTNACC 1 cut(s) 659
PspFI CCCAGC 3 cut(s) 182, 203, 365
PspPI GGNCC 1 cut(s) 335
PvuI CGATCG 1 cut(s) 108
RsaI GTAC 3 cut(s) 423, 428, 588
RsaNI GTAC 3 cut(s) 422, 427, 587
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 3 cut(s) 264, 609, 651
Sau3AI GATC 3 cut(s) 105, 109, 343
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 1 cut(s) 588
SduI GDGCHC 1 cut(s) 304
SetI ASST 8 cut(s) 51, 184, 205, 260, 340, 490, 503, 666
SfaNI GCATC 2 cut(s) 168, 229
SinI GGWCC 1 cut(s) 335
Sse9I AATT 5 cut(s) 34, 72, 268, 360, 411
SsiI CCGC 4 cut(s) 137, 195, 285, 297
SspMI CTAG 1 cut(s) 179
TaaI ACNGT 3 cut(s) 426, 460, 551
TaqI TCGA 4 cut(s) 108, 223, 325, 409
TasI AATT 5 cut(s) 34, 72, 268, 360, 411
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 2 cut(s) 187, 509
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 534
TseI GCWGC 3 cut(s) 263, 608, 650
Tsp45I GTSAC 1 cut(s) 534
TspDTI ATGAA 1 cut(s) 4
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 72
XspI CTAG 1 cut(s) 179
ZrmI AGTACT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.