FvH4_2g18530

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015193
Physical Location & Seq
Forward (+)
15903139 .. 15905032
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18530.t1

Sequence Viewer

Length: 597 bp
ATGGCTTCCGACGATCCCAAACCCTCACAATCAGAATCAGAATCAGAGCCTCCATCTTCGTCTTGGAAGGACCGCATCGTCTTCCCCACACTCGTCGCCGGAATCGTCGGCGCCGGAGCTGGGTTGGTCTCCAAACACCGGAAAGTCGTCGGCCTCCCAACCAGCTCCGCCACTTACGCCGCTAATTTCGCCATCGTCACTGCTTGTTACTGCGGGGCGCGTGAATATGTGGGAATAACGAGAAAGACGGGGCCTGATGATCTGCTCAACTCTGCTATCGCCGGCTTTGGCAGTGGTGCTATTCTTGGCCGGCTTTACGGCGGTCCAGTCTCTGCTGTCCGCTACTCAATACTCTTTACCATTGCTGGGACAACAGTGGACTATGCTACAATTAAACTACGACCTGTCTTGAAGAGCTACAAAGAATCTATGCTTGGGAGTAATGACGGTTCAAAGAAGAATGATGGTTGGCTGAAACTGCCCGACTGGTCCCCTATCAAAGTACTTGATGAAGAAGCCCTTGCTGCAAAGCAGGCTCGAGAAAAGCAGTTGTATGCACAGAGGGGACTTGGGAACTTCACCAAAGAAGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

199

Amino Acids

21.06

Weight (kDa)

8.98

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 53 - 121 9.8e-08 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 110
AccII CGCG 1 cut(s) 220
AciI CCGC 6 cut(s) 73, 168, 180, 213, 321, 340
AclWI GGATC 1 cut(s) 8
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 3 cut(s) 120, 138, 366
AgsI TTSAA 2 cut(s) 412, 453
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AluBI AGCT 3 cut(s) 119, 165, 417
AluI AGCT 3 cut(s) 119, 165, 417
Alw26I GTCTC 2 cut(s) 133, 334
AlwI GGATC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 537
AoxI GGCC 3 cut(s) 151, 251, 307
ApeKI GCWGC 1 cut(s) 524
AspLEI GCGC 2 cut(s) 113, 220
AspS9I GGNCC 4 cut(s) 70, 251, 323, 489
AsuHPI GGTGA 1 cut(s) 571
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 3 cut(s) 70, 323, 489
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 61, 200, 458
BceAI ACGGC 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 133, 334
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 2 cut(s) 180, 525
BlsI GCNGC 2 cut(s) 181, 526
BmcAI AGTACT 1 cut(s) 504
Bme18I GGWCC 3 cut(s) 70, 323, 489
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 4 cut(s) 70, 251, 323, 489
BmiI GGNNCC 3 cut(s) 112, 252, 491
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 138, 366
Bse118I RCCGGY 2 cut(s) 281, 309
Bse1I ACTGG 2 cut(s) 326, 491
Bse3DI GCAATG 1 cut(s) 360
BseLI CCNNNNNNNGG 3 cut(s) 120, 138, 366
BseMI GCAATG 1 cut(s) 360
BseNI ACTGG 2 cut(s) 326, 491
BseXI GCAGC 1 cut(s) 511
BseYI CCCAGC 2 cut(s) 119, 365
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 153, 253, 309
BshNI GGYRCC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 5 cut(s) 99, 114, 139, 282, 310
BslFI GGGAC 3 cut(s) 382, 475, 579
BslI CCNNNNNNNGG 3 cut(s) 120, 138, 366
BsmAI GTCTC 2 cut(s) 133, 334
BsmFI GGGAC 3 cut(s) 382, 475, 579
BsnI GGCC 3 cut(s) 153, 253, 309
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 537
Bsp143I GATC 2 cut(s) 13, 259
BspACI CCGC 6 cut(s) 73, 168, 180, 213, 321, 340
BspANI GGCC 3 cut(s) 153, 253, 309
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 112, 252, 491
BspPI GGATC 1 cut(s) 8
BspQI GCTCTTC 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 360
BsrFI RCCGGY 2 cut(s) 281, 309
BsrI ACTGG 2 cut(s) 326, 491
BssAI RCCGGY 2 cut(s) 281, 309
BssMI GATC 2 cut(s) 13, 259
BssNI GRCGYC 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 376, 449
Bst6I CTCTTC 2 cut(s) 407, 582
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 283, 311, 534
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 113, 220
BstKTI GATC 2 cut(s) 16, 262
BstMAI GTCTC 2 cut(s) 133, 334
BstMBI GATC 2 cut(s) 13, 259
BstMWI GCNNNNNNNGC 5 cut(s) 176, 188, 478, 524, 533
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 153, 253, 309
BtsI GCAGTG 2 cut(s) 198, 298
BtsIMutI CAGTG 3 cut(s) 198, 298, 381
Cac8I GCNNGC 3 cut(s) 283, 311, 534
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 2 cut(s) 113, 220
Cfr10I RCCGGY 2 cut(s) 281, 309
Cfr13I GGNCC 4 cut(s) 70, 251, 323, 489
Csp6I GTAC 1 cut(s) 503
CviQI GTAC 1 cut(s) 503
DinI GGCGCC 1 cut(s) 112
DpnI GATC 2 cut(s) 15, 261
DpnII GATC 2 cut(s) 13, 259
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 407, 582
EarI CTCTTC 2 cut(s) 407, 582
EciI GGCGGA 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 133
Eco47I GGWCC 3 cut(s) 70, 323, 489
Eco88I CYCGRG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 251
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
FaiI YATR 4 cut(s) 228, 384, 431, 555
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FaqI GGGAC 3 cut(s) 382, 475, 579
FauI CCCGC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 180, 525
Fsp4HI GCNGC 2 cut(s) 180, 525
GlaI GCGC 2 cut(s) 112, 219
GluI GCNGC 2 cut(s) 180, 525
GsaI CCCAGC 2 cut(s) 123, 369
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 3 cut(s) 153, 253, 309
HapII CCGG 5 cut(s) 99, 114, 139, 282, 310
HhaI GCGC 2 cut(s) 113, 220
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 2 cut(s) 111, 218
HinP1I GCGC 2 cut(s) 111, 218
HinfI GANTC 5 cut(s) 35, 41, 102, 425, 590
HpaII CCGG 5 cut(s) 99, 114, 139, 282, 310
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 10, 34, 40, 46
Hpy188III TCNNGA 2 cut(s) 409, 539
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 4 cut(s) 14, 98, 110, 152
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 376, 449
HpyCH4V TGCA 2 cut(s) 527, 557
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 188, 478, 524, 533
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 2 cut(s) 111, 218
KasI GGCGCC 1 cut(s) 110
KroI GCCGGC 2 cut(s) 281, 309
KroNI GCCGGC 2 cut(s) 283, 311
Kzo9I GATC 2 cut(s) 13, 259
LguI GCTCTTC 1 cut(s) 407
LmnI GCTCC 2 cut(s) 116, 170
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 196, 206
MalI GATC 2 cut(s) 15, 261
MboI GATC 2 cut(s) 13, 259
MboII GAAGA 5 cut(s) 48, 73, 424, 469, 524
MluCI AATT 2 cut(s) 184, 390
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 4 cut(s) 34, 60, 164, 555
MroNI GCCGGC 2 cut(s) 281, 309
MseI TTAA 2 cut(s) 393, 595
MspI CCGG 5 cut(s) 99, 114, 139, 282, 310
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 5 cut(s) 176, 188, 478, 524, 533
NaeI GCCGGC 2 cut(s) 283, 311
NarI GGCGCC 1 cut(s) 111
NdeII GATC 2 cut(s) 13, 259
NgoMIV GCCGGC 2 cut(s) 281, 309
NlaIV GGNNCC 3 cut(s) 112, 252, 491
NmuCI GTSAC 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 537
PciSI GCTCTTC 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 2 cut(s) 283, 311
PfeI GAWTC 4 cut(s) 35, 41, 102, 425
PkrI GCNGC 2 cut(s) 181, 526
PluTI GGCGCC 1 cut(s) 114
PspFI CCCAGC 2 cut(s) 119, 365
PspN4I GGNNCC 3 cut(s) 112, 252, 491
PspPI GGNCC 4 cut(s) 70, 251, 323, 489
PsrI GAACNNNNNNTAC 2 cut(s) 433, 465
PstNI CAGNNNCTG 1 cut(s) 332
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 407
SaqAI TTAA 2 cut(s) 393, 595
SatI GCNGC 2 cut(s) 180, 525
Sau3AI GATC 2 cut(s) 13, 259
Sau96I GGNCC 4 cut(s) 70, 251, 323, 489
ScaI AGTACT 1 cut(s) 504
SetI ASST 4 cut(s) 121, 167, 406, 419
SfaNI GCATC 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 537
SinI GGWCC 3 cut(s) 70, 323, 489
SlaI CTCGAG 1 cut(s) 537
SmlI CTYRAG 1 cut(s) 537
SmoI CTYRAG 1 cut(s) 537
Sse9I AATT 2 cut(s) 184, 390
SsiI CCGC 6 cut(s) 73, 168, 180, 213, 321, 340
SspDI GGCGCC 1 cut(s) 110
TaaI ACNGT 2 cut(s) 376, 449
TaqI TCGA 1 cut(s) 538
TasI AATT 2 cut(s) 184, 390
TatI WGTACW 1 cut(s) 502
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 4 cut(s) 35, 41, 102, 425
Tru1I TTAA 2 cut(s) 393, 595
Tru9I TTAA 2 cut(s) 393, 595
TscAI CASTG 3 cut(s) 205, 298, 381
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 525
TspRI CASTG 3 cut(s) 205, 298, 381
VpaK11BI GGWCC 3 cut(s) 70, 323, 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XhoI CTCGAG 1 cut(s) 537
ZrmI AGTACT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.