Rh6BG274100

Mitochondrial import inner membrane translocase subunit Tim17 Tim22 Tim23 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015193
Physical Location & Seq
Forward (+)
49487940 .. 49503536
15597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG274100.1

Sequence Viewer

Length: 717 bp
ATGGTTTGCACCGCTAATACCCGCATTTCCCTTAACAAAGCGAGGCCAGAGTGTTGGGGTGAGCTCAGGCGAGTCAAACAGCAAACACAATTTCAGAGAGCCCCTTCTTTCACTCTTGTTACTATGGCTTCCGACGATCCCAGAGCTTCAGACGCAGAATCAGAGGCTCCTCCATCTTCGTCTTGGAAGGACCGCATCGTCGTCCCCACACTCGTCGCCGGAATCGTTGGCGCCGGAGCTGGGTTGGTCTCCAAGCATCGGAAAGTCGTCGGCCTCCCAACCAGCTCCGCCACTTACGCTGCTAATTTCGCCATTGTCACAGCTTGTTACTGCGGGGCGCGTGAGTATGTAGGAATAACTAGAAAAACAGGGCCTGATGATCTGCTCAACTCTGCAATTGCTGGCTTTGGCAGTGGGGCTATTCTTGGGCGGCTTTACGGTGGTCCAGTTGCTGCTGTCCGCTACTCAATACTCTTTACTATTGCTGGGACAACATTGGACTATGCTACAATTAAACTACGACCTGTCTTGAAGAGCTACAAAGAATCTATGCTTGGGAGTAACGATGATTCAAAGAAGAATGATGGCTGGCTGAAACTGCCTGACTGGTCCCCTATCAAAGTACTTGATGAAGAAGCCCTTGCTGCAAAGCAGGCTCGAGAAAAGCAGTTATATGCACAGAGGGGACTTGGGAACTTCACCAAAGAAGAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

238

Amino Acids

25.65

Weight (kDa)

9.33

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 91 - 161 1.5e-06 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25120
fragaria_vesca FvH4_2g18530
malus_domestica MD05G1171500.v1.1 MD10G1159700.v1.1
prunus_persica Prupe.8G198800_v2.0.a1
pyrus_communis pycom05g15820 pycom10g13790
rosa_chinensis RchiOBHm_Chr6g0284091
rosa_laevigata RLG00000012770
rosa_roxburghii Rroxscaffold_7G00184940
rosa_rugosa Rorug06G0160600
rosa_samantha Rh6AG273400 Rh6BG274100 Rh6CG275500 Rh6DG268600
rosa_wichuraiana Rw6G023530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 230
AccII CGCG 1 cut(s) 340
AciI CCGC 7 cut(s) 12, 22, 193, 288, 333, 430, 460
AclWI GGATC 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 132
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 624
AfiI CCNNNNNNNGG 2 cut(s) 240, 258
AgsI TTSAA 2 cut(s) 532, 573
AjuI GAANNNNNNNTTGG 2 cut(s) 537, 569
AluBI AGCT 6 cut(s) 64, 146, 239, 285, 323, 537
AluI AGCT 6 cut(s) 64, 146, 239, 285, 323, 537
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 1 cut(s) 131
AlwNI CAGNNNCTG 2 cut(s) 374, 452
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 3 cut(s) 44, 271, 371
ApeKI GCWGC 3 cut(s) 299, 452, 644
AspLEI GCGC 2 cut(s) 233, 340
AspS9I GGNCC 4 cut(s) 190, 371, 443, 609
AsuHPI GGTGA 2 cut(s) 71, 691
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 3 cut(s) 190, 443, 609
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 2 cut(s) 66, 103
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 3 cut(s) 286, 439, 631
BccI CCATC 2 cut(s) 181, 578
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 234
BisI GCNGC 4 cut(s) 300, 431, 453, 645
BlsI GCNGC 4 cut(s) 301, 432, 454, 646
BmcAI AGTACT 1 cut(s) 624
Bme18I GGWCC 3 cut(s) 190, 443, 609
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 190, 371, 443, 609
BmiI GGNNCC 3 cut(s) 168, 232, 611
BmsI GCATC 2 cut(s) 204, 265
Bpu10I CCTNAGC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 253
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 258
Bse1I ACTGG 2 cut(s) 446, 611
BseLI CCNNNNNNNGG 2 cut(s) 240, 258
BseMII CTCAG 1 cut(s) 79
BseNI ACTGG 2 cut(s) 446, 611
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 3 cut(s) 286, 439, 631
BseYI CCCAGC 2 cut(s) 239, 485
Bsh1236I CGCG 1 cut(s) 340
BshFI GGCC 3 cut(s) 46, 273, 373
BshNI GGYRCC 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 2 cut(s) 219, 234
BslFI GGGAC 4 cut(s) 188, 502, 595, 699
BslI CCNNNNNNNGG 2 cut(s) 240, 258
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 4 cut(s) 188, 502, 595, 699
BsnI GGCC 3 cut(s) 46, 273, 373
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 2 cut(s) 66, 103
Bsp143I GATC 2 cut(s) 136, 379
BspACI CCGC 7 cut(s) 12, 22, 193, 288, 333, 430, 460
BspANI GGCC 3 cut(s) 46, 273, 373
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 168, 232, 611
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 253
BsrI ACTGG 2 cut(s) 446, 611
BssMI GATC 2 cut(s) 136, 379
BssNI GRCGYC 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 2 cut(s) 527, 702
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 3 cut(s) 403, 590, 654
BstDEI CTNAG 1 cut(s) 65
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 233, 340
BstKTI GATC 2 cut(s) 139, 382
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 2 cut(s) 136, 379
BstMWI GCNNNNNNNGC 6 cut(s) 152, 296, 308, 598, 644, 653
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 286, 439, 631
BstXI CCANNNNNNTGG 1 cut(s) 54
BsuRI GGCC 3 cut(s) 46, 273, 373
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 418
Cac8I GCNNGC 3 cut(s) 403, 590, 654
CaiI CAGNNNCTG 2 cut(s) 374, 452
CfoI GCGC 2 cut(s) 233, 340
Cfr13I GGNCC 4 cut(s) 190, 371, 443, 609
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 623
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 1 cut(s) 65
DinI GGCGCC 1 cut(s) 232
DpnI GATC 2 cut(s) 138, 381
DpnII GATC 2 cut(s) 136, 379
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 527, 702
EarI CTCTTC 2 cut(s) 527, 702
EciI GGCGGA 1 cut(s) 277
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 66, 103
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 3 cut(s) 190, 443, 609
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 657
EcoICRI GAGCTC 1 cut(s) 64
EcoO109I RGGNCCY 1 cut(s) 371
EcoT38I GRGCYC 2 cut(s) 66, 103
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
FaiI YATR 6 cut(s) 125, 348, 504, 551, 673, 675
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FaqI GGGAC 4 cut(s) 188, 502, 595, 699
FauI CCCGC 2 cut(s) 29, 326
Fnu4HI GCNGC 4 cut(s) 300, 431, 453, 645
FriOI GRGCYC 2 cut(s) 66, 103
Fsp4HI GCNGC 4 cut(s) 300, 431, 453, 645
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 232, 339
GluI GCNGC 4 cut(s) 300, 431, 453, 645
GsaI CCCAGC 2 cut(s) 243, 489
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 3 cut(s) 46, 273, 373
HapII CCGG 2 cut(s) 219, 234
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 233, 340
Hin1I GRCGYC 1 cut(s) 231
Hin6I GCGC 2 cut(s) 231, 338
HinP1I GCGC 2 cut(s) 231, 338
HinfI GANTC 6 cut(s) 72, 158, 222, 545, 569, 710
HpaII CCGG 2 cut(s) 219, 234
HphI GGTGA 2 cut(s) 71, 691
Hpy188I TCNGA 5 cut(s) 96, 133, 151, 163, 261
Hpy188III TCNNGA 2 cut(s) 529, 659
Hpy99I CGWCG 4 cut(s) 137, 203, 218, 272
HpyAV CCTTC 2 cut(s) 114, 181
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4V TGCA 4 cut(s) 9, 395, 647, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 296, 308, 598, 644, 653
HpyF3I CTNAG 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 231
HspAI GCGC 2 cut(s) 231, 338
KasI GGCGCC 1 cut(s) 230
Kzo9I GATC 2 cut(s) 136, 379
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 3 cut(s) 172, 236, 290
Lsp1109I GCAGC 3 cut(s) 286, 439, 631
LweI GCATC 2 cut(s) 204, 265
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 118, 316, 326, 560
MalI GATC 2 cut(s) 138, 381
MboI GATC 2 cut(s) 136, 379
MboII GAAGA 4 cut(s) 168, 544, 589, 644
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 2 cut(s) 66, 103
MluCI AATT 4 cut(s) 89, 304, 396, 510
Mly113I GGCGCC 1 cut(s) 231
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 5 cut(s) 36, 157, 180, 284, 675
MseI TTAA 3 cut(s) 33, 513, 715
MspI CCGG 2 cut(s) 219, 234
MunI CAATTG 1 cut(s) 396
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 6 cut(s) 152, 296, 308, 598, 644, 653
NarI GGCGCC 1 cut(s) 231
NdeII GATC 2 cut(s) 136, 379
NlaIV GGNNCC 3 cut(s) 168, 232, 611
NmuCI GTSAC 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 657
PciSI GCTCTTC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 4 cut(s) 158, 222, 545, 569
PkrI GCNGC 4 cut(s) 301, 432, 454, 646
PleI GAGTC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 239, 485
PspN4I GGNNCC 3 cut(s) 168, 232, 611
PspPI GGNCC 4 cut(s) 190, 371, 443, 609
PstNI CAGNNNCTG 2 cut(s) 374, 452
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 3 cut(s) 33, 513, 715
SatI GCNGC 4 cut(s) 300, 431, 453, 645
Sau3AI GATC 2 cut(s) 136, 379
Sau96I GGNCC 4 cut(s) 190, 371, 443, 609
ScaI AGTACT 1 cut(s) 624
SchI GAGTC 1 cut(s) 81
SduI GDGCHC 2 cut(s) 66, 103
SetI ASST 7 cut(s) 66, 148, 241, 287, 325, 526, 539
SfaNI GCATC 2 cut(s) 204, 265
SfoI GGCGCC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 3 cut(s) 190, 443, 609
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 4 cut(s) 89, 304, 396, 510
SsiI CCGC 7 cut(s) 12, 22, 193, 288, 333, 430, 460
SspDI GGCGCC 1 cut(s) 230
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 66
TaaI ACNGT 1 cut(s) 440
TaqI TCGA 1 cut(s) 658
TasI AATT 4 cut(s) 89, 304, 396, 510
TatI WGTACW 1 cut(s) 622
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 4 cut(s) 158, 222, 545, 569
Tru1I TTAA 3 cut(s) 33, 513, 715
Tru9I TTAA 3 cut(s) 33, 513, 715
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 299, 452, 644
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 645
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 3 cut(s) 190, 443, 609
XcmI CCANNNNNNNNNTGG 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 657
XspI CTAG 1 cut(s) 360
ZrmI AGTACT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.