Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010242

Physical Location & Seq

Genomic Coordinates Reverse (-)

9156822 .. 9160100

Gene Structure 3279 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G25150.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGCGATGTTAGGTGCACAGCAAGTTCCAGCAGCAGCTTGTACTCCAGATATGGTTGGGAATGCTTTTGTGCCCCAGTATTATCACATATTGCATCAATCACCTGAGCATGTTCACAGATTTTACCAAGAGATTAGCAAGTTAGGTCGTCCTGAAGAGAATGGTTTAATGAGCATCACTTCTACCTTGCAAGCTATTGACAAGAAGATAATGGCGCTTGGTTACGGTGTAATCAGTGCAGAGATAGCTACTGTGGACACACAAGAATCTCATGGAGGTGGTTATATTGTACTGGTGACTGGGTATTTGACGGGAAAAGACAGTGTCAGGAGGACGTTTAGTCAGACCTTCTTCCTTGCTCCACAGGAGACAGGATACTTTGTCTTGAATGATATGTTTCGATTCATTGATGAAGGCACTGTCGTACATGGAAATCAGATTCCAGTGAACAACGTCCAAGCTCCTGTCAACACTTACCAGGACACAGCTGCTGCGAAGGAAATTCCAGATGACTTTGTTCAGGAGAAATATGTCCAAGAGAATCATGCTGTTAAGCAAACCGAGGTGTTGTCCAAGAGCATTAATGAGCCTGAAAAAGTGTTCACGCCCTCTGAAGATGAACAAGTATCAGCTGCAGAAGAAGCTCTGGTGACTGAAACAGTTAATGAAGCACCAATTGAAGTGCAAAAGGTTGGAGAATCTGATTCTAGGACTGGCGAAATTCCAAAGAGATCTTATGCATCAATTGTGAAGGTTATGAAAGAAAATGCTGCACCAATGTCTGCTTCGAGAACTCCAACAAAGGTGGAACCAAAGAAACAAGAAGATCAAGCCATTCATATCCCTCTACCAACACCATTGTCTGAGAAATCAGATTCAGGAGCAAATGTTGCTGTAAATGAGAACAATCAAGAGAATGAAAGAGCTCTAGGTCCATCCATCTATCTAAAGGGTTTACCCCTTGATGCAACACCTGCCTTGCTTGAGAATGAGTTCCAGAAATTTGGACTTATTAGGACCAATGGAATTCAAGTGAGAAGCCAGAAGGGATTCTGTTTTGGTTTTGTTGAGTTTGAATCCGCAAGTTCCATGCAAAGCGCTATCGAGGCATCACCTGTCATGCTCAATGGACACAAAGTTGTTGTGGAGGAAAAGCGATCTACCGCAAGAGGGAACTATAGAGGACGTTCGACGTTTGGTGTAAACACAGGCTACAGAAACGAAGGAGGAAGGGGTCGTGGGAGCTTTGGAGGTGGAAGAGGAGGATATGGCCGGACCGATTTCAACGGATATGGTAATAACAGGGGAAACAATAGAGGCGGATACGCAAACCGAGCAAATGGTGATGGTGGTGGGTTCCCGAGGGCCAATGGTAACAATGGACGAGTAAGACGTGGTGGCGGAAATGATGCTAACAGAGCTACGAAACCCGTGGATGATGCTCCCCGTGTGTCTGTTGCTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006807 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0010494 GO:0016236 GO:0016579 GO:0019538 GO:0032386 GO:0032879 GO:0032991 GO:0034517 GO:0035770 GO:0035973 GO:0036211 GO:0036464 GO:0043021 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051049 GO:0060341 GO:0060627 GO:0060628 GO:0061912 GO:0061919 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1905538 GO:1990861 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTF2 RRM_1

Protein Analysis

488

Amino Acids

52.87

Weight (kDa)

6.07

Isoelectric Point (pI)

43.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTF2	PF02136	118 - 234	1.7e-34	Nuclear transport factor 2 (NTF2) domain
RRM_1	PF00076	413 - 470	3.8e-09	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010242)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25150 AT3G25150
fragaria_vesca	FvH4_2g18460 FvH4_2g18460 FvH4_2g18460
malus_domestica	MD05G1170400.v1.1 MD10G1158600.v1.1
prunus_persica	Prupe.8G197600_v2.0.a1
pyrus_communis	pycom05g15760 pycom10g13710
rosa_chinensis	RchiOBHm_Chr6g0283941
rosa_laevigata	RLG00000012785
rosa_multiflora	Rmu_ssc0000442.1_g000025
rosa_roxburghii	Rroxscaffold_7G00185040
rosa_rugosa	Rorug06G0158600 Rorug06G0158700
rosa_samantha	Rh6AG271900 Rh6BG272800 Rh6CG274000 Rh6DG267000
rosa_wichuraiana	Rw6G023430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	982
Acc36I	ACCTGC	1 cut(s)	982
AciI	CCGC	4 cut(s)	1080, 1164, 1320, 1401
AcoI	YGGCCR	1 cut(s)	1270
AcsI	RAATTY	4 cut(s)	501, 720, 1001, 1026
AcuI	CTGAAG	2 cut(s)	174, 633
AfaI	GTAC	3 cut(s)	43, 291, 426
AfeI	AGCGCT	1 cut(s)	1099
AfiI	CCNNNNNNNGG	1 cut(s)	1170
AgsI	TTSAA	5 cut(s)	388, 680, 1031, 1076, 1285
AhdI	GACNNNNNGTC	1 cut(s)	339
AjiI	CACGTC	1 cut(s)	1394
AjnI	CCWGG	1 cut(s)	477
AjuI	GAANNNNNNNTTGG	2 cut(s)	769, 801
Alw21I	GWGCWC	2 cut(s)	19, 928
Alw26I	GTCTC	1 cut(s)	362
Alw44I	GTGCAC	1 cut(s)	15
AlwNI	CAGNNNCTG	1 cut(s)	491
Ama87I	CYCGRG	1 cut(s)	1360
Aor51HI	AGCGCT	1 cut(s)	1099
AoxI	GGCC	2 cut(s)	1270, 1365
ApaLI	GTGCAC	1 cut(s)	15
ApeKI	GCWGC	7 cut(s)	32, 35, 488, 491, 632, 770, 1460
ApoI	RAATTY	4 cut(s)	501, 720, 1001, 1026
ArsI	GACNNNNNNTTYG	2 cut(s)	289, 321
AseI	ATTAAT	1 cut(s)	582
Asp700I	GAANNNNTTC	2 cut(s)	992, 1049
AspLEI	GCGC	2 cut(s)	217, 1100
AspS9I	GGNCC	4 cut(s)	932, 1017, 1275, 1365
AsuHPI	GGTGA	5 cut(s)	93, 307, 661, 1104, 1355
AvaI	CYCGRG	1 cut(s)	1360
AvaII	GGWCC	3 cut(s)	932, 1017, 1275
BaeGI	GKGCMC	2 cut(s)	19, 75
BanII	GRGCYC	1 cut(s)	928
Bbv12I	GWGCWC	2 cut(s)	19, 928
BbvI	GCAGC	7 cut(s)	44, 47, 475, 478, 619, 757, 1447
BccI	CCATC	3 cut(s)	943, 947, 1340
BciT130I	CCWGG	1 cut(s)	479
BciVI	GTATCC	2 cut(s)	368, 1316
BcoDI	GTCTC	1 cut(s)	362
BfaI	CTAG	2 cut(s)	708, 929
BfmI	CTRYAG	3 cut(s)	633, 1177, 1213
BfoI	RGCGCY	2 cut(s)	218, 1101
BfuAI	ACCTGC	1 cut(s)	982
BfuI	GTATCC	2 cut(s)	368, 1316
BglII	AGATCT	1 cut(s)	731
BisI	GCNGC	7 cut(s)	33, 36, 489, 492, 633, 771, 1461
BlsI	GCNGC	7 cut(s)	34, 37, 490, 493, 634, 772, 1462
Bme1390I	CCNGG	1 cut(s)	479
Bme18I	GGWCC	3 cut(s)	932, 1017, 1275
BmeRI	GACNNNNNGTC	1 cut(s)	339
BmeT110I	CYCGRG	1 cut(s)	1360
BmgBI	CACGTC	1 cut(s)	1394
BmgT120I	GGNCC	4 cut(s)	932, 1017, 1275, 1365
BmiI	GGNNCC	2 cut(s)	810, 1358
BmrFI	CCNGG	1 cut(s)	479
BmrI	ACTGGG	2 cut(s)	70, 309
BmsI	GCATC	7 cut(s)	103, 183, 749, 955, 1118, 1399, 1429
BmuI	ACTGGG	2 cut(s)	70, 309
BpmI	CTGGAG	1 cut(s)	30
Bpu10I	CCTNAGC	1 cut(s)	105
BpuEI	CTTGAG	1 cut(s)	1004
BsaJI	CCNNGG	3 cut(s)	561, 1361, 1431
BsaXI	ACNNNNNCTCC	2 cut(s)	873, 903
Bsc4I	CCNNNNNNNGG	1 cut(s)	1170
Bse1I	ACTGG	5 cut(s)	76, 297, 304, 443, 718
BseBI	CCWGG	1 cut(s)	479
BseDI	CCNNGG	3 cut(s)	561, 1361, 1431
BseGI	GGATG	2 cut(s)	935, 1441
BseLI	CCNNNNNNNGG	1 cut(s)	1170
BseMII	CTCAG	2 cut(s)	96, 855
BseNI	ACTGG	5 cut(s)	76, 297, 304, 443, 718
BseRI	GAGGAG	1 cut(s)	1275
BseSI	GKGCMC	2 cut(s)	19, 75
BseXI	GCAGC	7 cut(s)	44, 47, 475, 478, 619, 757, 1447
BsgI	GTGCAG	2 cut(s)	258, 756
BshFI	GGCC	2 cut(s)	1272, 1367
BsiHKAI	GWGCWC	2 cut(s)	19, 928
BsiHKCI	CYCGRG	1 cut(s)	1360
BsiSI	CCGG	1 cut(s)	1273
BslI	CCNNNNNNNGG	1 cut(s)	1170
BsmAI	GTCTC	1 cut(s)	362
BsmI	GAATGC	1 cut(s)	67
BsnI	GGCC	2 cut(s)	1272, 1367
BsoBI	CYCGRG	1 cut(s)	1360
Bsp1286I	GDGCHC	3 cut(s)	19, 75, 928
Bsp143I	GATC	3 cut(s)	731, 826, 1157
BspACI	CCGC	4 cut(s)	1080, 1164, 1320, 1401
BspANI	GGCC	2 cut(s)	1272, 1367
BspCNI	CTCAG	2 cut(s)	97, 856
BspLI	GGNNCC	2 cut(s)	810, 1358
BspMAI	CTGCAG	1 cut(s)	637
BspMI	ACCTGC	1 cut(s)	982
BsrI	ACTGG	5 cut(s)	76, 297, 304, 443, 718
BssECI	CCNNGG	3 cut(s)	561, 1361, 1431
BssMI	GATC	3 cut(s)	731, 826, 1157
Bst2UI	CCWGG	1 cut(s)	479
Bst4CI	ACNGT	5 cut(s)	227, 253, 323, 421, 661
Bst6I	CTCTTC	2 cut(s)	150, 1252
BstAPI	GCANNNNNTGC	2 cut(s)	890, 974
BstC8I	GCNNGC	1 cut(s)	192
BstDEI	CTNAG	2 cut(s)	105, 864
BstDSI	CCRYGG	1 cut(s)	1431
BstF5I	GGATG	2 cut(s)	935, 1441
BstH2I	RGCGCY	2 cut(s)	218, 1101
BstHHI	GCGC	2 cut(s)	217, 1100
BstKTI	GATC	3 cut(s)	734, 829, 1160
BstMAI	GTCTC	1 cut(s)	362
BstMBI	GATC	3 cut(s)	731, 826, 1157
BstMWI	GCNNNNNNNGC	7 cut(s)	245, 641, 890, 974, 1106, 1334, 1418
BstNI	CCWGG	1 cut(s)	479
BstNSI	RCATGY	1 cut(s)	113
BstSCI	CCNGG	1 cut(s)	477
BstSFI	CTRYAG	3 cut(s)	633, 1177, 1213
BstSLI	GKGCMC	2 cut(s)	19, 75
BstV1I	GCAGC	7 cut(s)	44, 47, 475, 478, 619, 757, 1447
BstX2I	RGATCY	1 cut(s)	731
BstXI	CCANNNNNNTGG	1 cut(s)	1004
BstYI	RGATCY	1 cut(s)	731
BsuI	GTATCC	2 cut(s)	368, 1316
BsuRI	GGCC	2 cut(s)	1272, 1367
BtgI	CCRYGG	1 cut(s)	1431
BtgZI	GCGATG	1 cut(s)	20
BtrI	CACGTC	1 cut(s)	1394
BtsCI	GGATG	2 cut(s)	935, 1441
BtsIMutI	CAGTG	4 cut(s)	241, 328, 417, 450
BveI	ACCTGC	1 cut(s)	982
Cac8I	GCNNGC	1 cut(s)	192
CaiI	CAGNNNCTG	1 cut(s)	491
CfoI	GCGC	2 cut(s)	217, 1100
Cfr13I	GGNCC	4 cut(s)	932, 1017, 1275, 1365
CpoI	CGGWCCG	1 cut(s)	1275
Csp6I	GTAC	3 cut(s)	42, 290, 425
CspCI	CAANNNNNGTGG	2 cut(s)	786, 821
CspI	CGGWCCG	1 cut(s)	1275
CviAII	CATG	6 cut(s)	110, 272, 428, 545, 1090, 1120
CviQI	GTAC	3 cut(s)	42, 290, 425
DdeI	CTNAG	2 cut(s)	105, 864
DpnI	GATC	3 cut(s)	733, 828, 1159
DpnII	GATC	3 cut(s)	731, 826, 1157
DriI	GACNNNNNGTC	1 cut(s)	339
EaeI	YGGCCR	1 cut(s)	1270
Eam1104I	CTCTTC	2 cut(s)	150, 1252
Eam1105I	GACNNNNNGTC	1 cut(s)	339
EarI	CTCTTC	2 cut(s)	150, 1252
EciI	GGCGGA	2 cut(s)	1335, 1416
Ecl136II	GAGCTC	1 cut(s)	926
Eco24I	GRGCYC	1 cut(s)	928
Eco47I	GGWCC	3 cut(s)	932, 1017, 1275
Eco47III	AGCGCT	1 cut(s)	1099
Eco53kI	GAGCTC	1 cut(s)	926
Eco57I	CTGAAG	2 cut(s)	174, 633
Eco88I	CYCGRG	1 cut(s)	1360
EcoICRI	GAGCTC	1 cut(s)	926
EcoRI	GAATTC	1 cut(s)	1026
EcoRII	CCWGG	1 cut(s)	477
EcoT22I	ATGCAT	1 cut(s)	742
EcoT38I	GRGCYC	1 cut(s)	928
FaeI	CATG	6 cut(s)	113, 275, 431, 548, 1093, 1123
FatI	CATG	6 cut(s)	109, 271, 427, 544, 1089, 1119
Fnu4HI	GCNGC	7 cut(s)	33, 36, 489, 492, 633, 771, 1461
FokI	GGATG	2 cut(s)	922, 1448
FriOI	GRGCYC	1 cut(s)	928
Fsp4HI	GCNGC	7 cut(s)	33, 36, 489, 492, 633, 771, 1461
FspBI	CTAG	2 cut(s)	708, 929
GlaI	GCGC	2 cut(s)	216, 1099
GluI	GCNGC	7 cut(s)	33, 36, 489, 492, 633, 771, 1461
GsuI	CTGGAG	1 cut(s)	30
HaeII	RGCGCY	2 cut(s)	218, 1101
HaeIII	GGCC	2 cut(s)	1272, 1367
HapII	CCGG	1 cut(s)	1273
HhaI	GCGC	2 cut(s)	217, 1100
Hin1II	CATG	6 cut(s)	113, 275, 431, 548, 1093, 1123
Hin6I	GCGC	2 cut(s)	215, 1098
HinP1I	GCGC	2 cut(s)	215, 1098
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HinfI	GANTC	9 cut(s)	266, 402, 439, 541, 698, 704, 875, 1050, 1076
HpaII	CCGG	1 cut(s)	1273
HphI	GGTGA	5 cut(s)	93, 307, 661, 1104, 1355
Hpy166II	GTNNAC	8 cut(s)	17, 115, 256, 448, 469, 603, 956, 1204
Hpy188I	TCNGA	6 cut(s)	345, 438, 613, 703, 865, 874
Hpy8I	GTNNAC	8 cut(s)	17, 115, 256, 448, 469, 603, 956, 1204
Hpy99I	CGWCG	1 cut(s)	1195
HpyAV	CCTTC	7 cut(s)	358, 407, 490, 745, 1039, 1217, 1224
HpyCH4III	ACNGT	5 cut(s)	227, 253, 323, 421, 661
HpyCH4IV	ACGT	5 cut(s)	335, 453, 1186, 1193, 1393
HpyF10VI	GCNNNNNNNGC	7 cut(s)	245, 641, 890, 974, 1106, 1334, 1418
HpyF3I	CTNAG	2 cut(s)	105, 864
HpySE526I	ACGT	5 cut(s)	335, 453, 1186, 1193, 1393
Hsp92II	CATG	6 cut(s)	113, 275, 431, 548, 1093, 1123
HspAI	GCGC	2 cut(s)	215, 1098
Kzo9I	GATC	3 cut(s)	731, 826, 1157
LmnI	GCTCC	5 cut(s)	364, 466, 881, 1242, 1447
Lsp1109I	GCAGC	7 cut(s)	44, 47, 475, 478, 619, 757, 1447
LweI	GCATC	7 cut(s)	103, 183, 749, 955, 1118, 1399, 1429
MaeI	CTAG	2 cut(s)	708, 929
MaeII	ACGT	5 cut(s)	335, 453, 1186, 1193, 1393
MaeIII	GTNAC	4 cut(s)	221, 295, 649, 1373
MalI	GATC	3 cut(s)	733, 828, 1159
MboI	GATC	3 cut(s)	731, 826, 1157
MboII	GAAGA	7 cut(s)	167, 217, 343, 626, 650, 836, 1269
MfeI	CAATTG	2 cut(s)	675, 744
MflI	RGATCY	1 cut(s)	731
MhlI	GDGCHC	3 cut(s)	19, 75, 928
MluCI	AATT	6 cut(s)	501, 675, 720, 744, 1001, 1026
MmeI	TCCRAC	2 cut(s)	673, 821
Mph1103I	ATGCAT	1 cut(s)	742
MroXI	GAANNNNTTC	2 cut(s)	992, 1049
MseI	TTAA	4 cut(s)	167, 552, 582, 663
MslI	CAYNNNNRTG	1 cut(s)	276
MspA1I	CMGCKG	2 cut(s)	488, 632
MspI	CCGG	1 cut(s)	1273
MspR9I	CCNGG	1 cut(s)	479
MunI	CAATTG	2 cut(s)	675, 744
Mva1269I	GAATGC	1 cut(s)	67
MvaI	CCWGG	1 cut(s)	479
MwoI	GCNNNNNNNGC	7 cut(s)	245, 641, 890, 974, 1106, 1334, 1418
NdeII	GATC	3 cut(s)	731, 826, 1157
NlaIII	CATG	6 cut(s)	113, 275, 431, 548, 1093, 1123
NlaIV	GGNNCC	2 cut(s)	810, 1358
NmuCI	GTSAC	2 cut(s)	295, 649
NsiI	ATGCAT	1 cut(s)	742
NspI	RCATGY	1 cut(s)	113
PaqCI	CACCTGC	1 cut(s)	982
PcsI	WCGNNNNNNNCGW	1 cut(s)	1390
PctI	GAATGC	1 cut(s)	67
PdmI	GAANNNNTTC	2 cut(s)	992, 1049
PfeI	GAWTC	9 cut(s)	266, 402, 439, 541, 698, 704, 875, 1050, 1076
PflFI	GACNNNGTC	1 cut(s)	323
PkrI	GCNGC	7 cut(s)	34, 37, 490, 493, 634, 772, 1462
PshBI	ATTAAT	1 cut(s)	582
Psp124BI	GAGCTC	1 cut(s)	928
Psp6I	CCWGG	1 cut(s)	477
PspGI	CCWGG	1 cut(s)	477
PspN4I	GGNNCC	2 cut(s)	810, 1358
PspPI	GGNCC	4 cut(s)	932, 1017, 1275, 1365
PstI	CTGCAG	1 cut(s)	637
PstNI	CAGNNNCTG	1 cut(s)	491
PsuI	RGATCY	1 cut(s)	731
PsyI	GACNNNGTC	1 cut(s)	323
PvuII	CAGCTG	2 cut(s)	488, 632
RsaI	GTAC	3 cut(s)	43, 291, 426
RsaNI	GTAC	3 cut(s)	42, 290, 425
RseI	CAYNNNNRTG	1 cut(s)	276
Rsr2I	CGGWCCG	1 cut(s)	1275
RsrII	CGGWCCG	1 cut(s)	1275
SacI	GAGCTC	1 cut(s)	928
SaqAI	TTAA	4 cut(s)	167, 552, 582, 663
SatI	GCNGC	7 cut(s)	33, 36, 489, 492, 633, 771, 1461
Sau3AI	GATC	3 cut(s)	731, 826, 1157
Sau96I	GGNCC	4 cut(s)	932, 1017, 1275, 1365
ScrFI	CCNGG	1 cut(s)	479
SduI	GDGCHC	3 cut(s)	19, 75, 928
SfaNI	GCATC	7 cut(s)	103, 183, 749, 955, 1118, 1399, 1429
SfcI	CTRYAG	3 cut(s)	633, 1177, 1213
SinI	GGWCC	3 cut(s)	932, 1017, 1275
SmiMI	CAYNNNNRTG	1 cut(s)	276
SmlI	CTYRAG	1 cut(s)	983
SmoI	CTYRAG	1 cut(s)	983
Sse9I	AATT	6 cut(s)	501, 675, 720, 744, 1001, 1026
SsiI	CCGC	4 cut(s)	1080, 1164, 1320, 1401
SspMI	CTAG	2 cut(s)	708, 929
SstI	GAGCTC	1 cut(s)	928
StyD4I	CCNGG	1 cut(s)	477
TaaI	ACNGT	5 cut(s)	227, 253, 323, 421, 661
TaiI	ACGT	5 cut(s)	338, 456, 1189, 1196, 1396
TaqI	TCGA	4 cut(s)	400, 788, 1104, 1190
TaqII	GACCGA	1 cut(s)	1292
TasI	AATT	6 cut(s)	501, 675, 720, 744, 1001, 1026
TatI	WGTACW	2 cut(s)	41, 289
TfiI	GAWTC	9 cut(s)	266, 402, 439, 541, 698, 704, 875, 1050, 1076
Tru1I	TTAA	4 cut(s)	167, 552, 582, 663
Tru9I	TTAA	4 cut(s)	167, 552, 582, 663
TscAI	CASTG	4 cut(s)	241, 328, 424, 450
TseFI	GTSAC	2 cut(s)	295, 649
TseI	GCWGC	7 cut(s)	32, 35, 488, 491, 632, 770, 1460
Tsp45I	GTSAC	2 cut(s)	295, 649
TspDTI	ATGAA	7 cut(s)	394, 426, 633, 681, 773, 827, 933
TspGWI	ACGGA	1 cut(s)	1302
TspRI	CASTG	4 cut(s)	241, 328, 424, 450
Tth111I	GACNNNGTC	1 cut(s)	323
VneI	GTGCAC	1 cut(s)	15
VpaK11BI	GGWCC	3 cut(s)	932, 1017, 1275
VspI	ATTAAT	1 cut(s)	582
XapI	RAATTY	4 cut(s)	501, 720, 1001, 1026
XceI	RCATGY	1 cut(s)	113
XmnI	GAANNNNTTC	2 cut(s)	992, 1049
XspI	CTAG	2 cut(s)	708, 929
Zsp2I	ATGCAT	1 cut(s)	742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.