AT3G25250

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010975
Physical Location & Seq
Forward (+)
9195334 .. 9197126
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25250.1

Sequence Viewer

Length: 1266 bp
ATGCTAGAGGGAGATGAGAAACAGAGCCGAGCTCTGGATTTCAATCGACTCGAGGTTTTGTCATTACTAGGTCGTGGAGCTAAAGGAGTTGTGTTTCTTGTCCGCGACGACGACGCTAAATTGCTTGCTTTAAAAGTTATACTTAAGGAAGCCATCGAGAAGAAAAAGAAGGGTAGAGAAAGTGAAGATGATGAGTATAAACGCGTGAGTTTCGAGCAAGGAGTATTAAGCAGATTCGACCATCCTCTCTTTCCGTCTCTTCACGGTGTTTTAGCTACCGATAAAGTCATCGGTTATGCGATCGATTATTGTCCGGGACAGAATCTCAATTCTTTGAGGAAAATGCAATCGGAAAGTATGTTTTCCGACGAGATTATCAGATTTTACGCGGCGGAGCTTGTATTAGCACTTGACTATTTACATAATCAAGGAATTGTGTATAGAGATTTGAAGCCAGACAATGTGATGATCCAAGAAAATGGACATTTAATGCTTATAGATTTCGATCTTTCTACAAATCTAGCTCCAAGAACGCCGCAACCGTCACCGTCACTGTCTAAACCATCGCCGACGATGAAGAGAAAGAAACGTCTCTTCCGCTTCACCAGTTTCTGCAACTCAGGAATCTCGCCGCAAGAATCTATCTCGGTTCACTCATCATCTACATTGGCCGTGTCAGATTCTTCAGGTGAGAAGTCAAATTCTTTCGTCGGAACAGAGGAATACGTTGCGCCGGAGGTTATAAGCGGGGACGGTCACGATTTTGCCGTCGATTGGTGGTCGTTAGGGGTTGTTTTATATGAGATGTTGTATGGAGCGACGCCGTTTAGAGGATCGAACCGGAAAGAGACATTTTACCGGATTTTATCTAAACCGCCGAATCTAACTGGTGAAACGACGTCGTTGCGTGATTTGATTAGAAGATTGTTGGAGAAAGATCCTAGCCGTAGGATCAACGTTGAGGAAATCAAGGGTCATGATTTCTTCAGAGGAGTAGATTGGGAAAAGGTGATTTTGGTTTCTAGACCGCCGTATATCCCGGCGCCGGACGACGGTGGTGATAAGGGAACCGATGTAAATACGAAGATGGATGTGGAGAATATCGTCCAAGAGATTTTTGCTGCAAGACAAGAACGTGAGAAACAGAGTGGTGATAACAATAAAAACGCTAATATGAAAATTAAAGATAATACTAGTGGGGAATGGGTTAAGGGGTTGAATAATAATCACGATTTAGAAAGTGATAACAATTTTTTGGTATTTTAA

Protein Analysis

421

Amino Acids

47.56

Weight (kDa)

5.66

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 18 - 178 3.3e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 174 1.5e-12 Protein tyrosine and serine/threonine kinase
YrbL-PhoP_reg PF10707 21 - 164 1.7e-06 PhoP regulatory network protein YrbL
Pkinase PF00069 229 - 329 2.2e-23 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 238 - 324 2.9e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010975)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25250 AT4G13000
fragaria_vesca FvH4_2g18040
malus_domestica MD05G1164900.v1.1 MD10G1154200.v1.1
prunus_persica Prupe.8G193100_v2.0.a1
pyrus_communis pycom05g15350 pycom10g13330
rosa_chinensis RchiOBHm_Chr6g0283431
rosa_laevigata RLG00000012827
rosa_multiflora Rmu_sc0003166.1_g000017 Rmu_sc0004959.1_g000020 Rmu_sc0039373.1_g000001
rosa_roxburghii Rroxscaffold_7G00185540
rosa_rugosa Rorug06G0154100
rosa_samantha Rh6AG266400 Rh6BG268900 Rh6CG268800 Rh6DG262200
rosa_wichuraiana Rw6G023060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AatII GACGTC 1 cut(s) 902
AccB1I GGYRCC 1 cut(s) 1042
AccII CGCG 3 cut(s) 105, 204, 389
AciI CCGC 9 cut(s) 103, 389, 392, 536, 598, 632, 747, 875, 1028
AclI AACGTT 1 cut(s) 957
AclWI GGATC 4 cut(s) 463, 841, 932, 959
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 2 cut(s) 669, 970
AcyI GRCGYC 3 cut(s) 821, 899, 1043
AfiI CCNNNNNNNGG 5 cut(s) 34, 774, 830, 1045, 1052
AflII CTTAAG 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 3 cut(s) 43, 451, 1219
AhlI ACTAGT 1 cut(s) 1193
AluBI AGCT 5 cut(s) 32, 80, 275, 397, 524
AluI AGCT 5 cut(s) 32, 80, 275, 397, 524
Alw21I GWGCWC 1 cut(s) 34
Alw26I GTCTC 3 cut(s) 261, 596, 842
AlwI GGATC 4 cut(s) 463, 841, 932, 959
AlwNI CAGNNNCTG 1 cut(s) 612
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 1 cut(s) 669
ApeKI GCWGC 1 cut(s) 1121
ApoI RAATTY 1 cut(s) 700
AspLEI GCGC 2 cut(s) 733, 1045
AsuC2I CCSGG 2 cut(s) 315, 1040
AsuHPI GGTGA 7 cut(s) 537, 595, 701, 902, 1021, 1070, 1163
AvaI CYCGRG 1 cut(s) 50
BanI GGYRCC 1 cut(s) 1042
BanII GRGCYC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 1108
BccI CCATC 4 cut(s) 161, 249, 571, 1081
BceAI ACGGC 5 cut(s) 656, 752, 808, 930, 1015
BcgI CGANNNNNNTGC 2 cut(s) 886, 920
BcnI CCSGG 2 cut(s) 315, 1040
BcoDI GTCTC 3 cut(s) 261, 596, 842
BcuI ACTAGT 1 cut(s) 1193
BfaI CTAG 6 cut(s) 5, 68, 521, 942, 1023, 1194
BfoI RGCGCY 1 cut(s) 1046
BfrI CTTAAG 1 cut(s) 143
BisI GCNGC 4 cut(s) 390, 536, 632, 1122
BlsI GCNGC 4 cut(s) 391, 537, 633, 1123
Bme1390I CCNGG 2 cut(s) 315, 1040
BmeT110I CYCGRG 1 cut(s) 50
BmiI GGNNCC 2 cut(s) 1044, 1069
BmrFI CCNGG 2 cut(s) 315, 1040
BplI GAGNNNNNCTC 2 cut(s) 16, 48
BpuMI CCSGG 2 cut(s) 315, 1040
Bsa29I ATCGAT 1 cut(s) 303
BsaBI GATNNNNATC 2 cut(s) 42, 504
BsaHI GRCGYC 3 cut(s) 821, 899, 1043
BsaWI WCCGGW 2 cut(s) 840, 858
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 774, 830, 1045, 1052
Bse1I ACTGG 2 cut(s) 606, 892
Bse8I GATNNNNATC 2 cut(s) 42, 504
BseCI ATCGAT 1 cut(s) 303
BseGI GGATG 2 cut(s) 241, 1096
BseJI GATNNNNATC 2 cut(s) 42, 504
BseLI CCNNNNNNNGG 5 cut(s) 34, 774, 830, 1045, 1052
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 2 cut(s) 606, 892
BseRI GAGGAG 1 cut(s) 1005
BseXI GCAGC 1 cut(s) 1108
Bsh1236I CGCG 3 cut(s) 105, 204, 389
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 1 cut(s) 671
BshNI GGYRCC 1 cut(s) 1042
BshVI ATCGAT 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 6 cut(s) 314, 734, 841, 859, 1040, 1046
BslFI GGGAC 2 cut(s) 330, 764
BslI CCNNNNNNNGG 5 cut(s) 34, 774, 830, 1045, 1052
BsmAI GTCTC 3 cut(s) 261, 596, 842
BsmBI CGTCTC 2 cut(s) 261, 596
BsmFI GGGAC 2 cut(s) 330, 764
BsnI GGCC 1 cut(s) 671
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 34
Bsp143I GATC 6 cut(s) 300, 468, 505, 833, 937, 951
BspACI CCGC 9 cut(s) 103, 389, 392, 536, 598, 632, 747, 875, 1028
BspANI GGCC 1 cut(s) 671
BspCNI CTCAG 1 cut(s) 632
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 3 cut(s) 105, 204, 389
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 2 cut(s) 1044, 1069
BspPI GGATC 4 cut(s) 463, 841, 932, 959
BspT107I GGYRCC 1 cut(s) 1042
BspTI CTTAAG 1 cut(s) 143
BsrI ACTGG 2 cut(s) 606, 892
BssMI GATC 6 cut(s) 300, 468, 505, 833, 937, 951
BssNI GRCGYC 3 cut(s) 821, 899, 1043
Bst4CI ACNGT 6 cut(s) 266, 543, 549, 555, 755, 1055
Bst6I CTCTTC 3 cut(s) 264, 572, 599
BstACI GRCGYC 3 cut(s) 821, 899, 1043
BstAFI CTTAAG 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 619
BstF5I GGATG 2 cut(s) 241, 1096
BstFNI CGCG 3 cut(s) 105, 204, 389
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 2 cut(s) 733, 1045
BstKTI GATC 6 cut(s) 303, 471, 508, 836, 940, 954
BstMAI GTCTC 3 cut(s) 261, 596, 842
BstMBI GATC 6 cut(s) 300, 468, 505, 833, 937, 951
BstMCI CGRYCG 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 313, 1038
BstUI CGCG 3 cut(s) 105, 204, 389
BstV1I GCAGC 1 cut(s) 1108
BstX2I RGATCY 1 cut(s) 937
BstXI CCANNNNNNTGG 1 cut(s) 479
BstYI RGATCY 1 cut(s) 937
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 1 cut(s) 671
BsuTUI ATCGAT 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 241, 1096
BtsIMutI CAGTG 1 cut(s) 551
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 612
CciI TCATGA 1 cut(s) 976
CfoI GCGC 2 cut(s) 733, 1045
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 2 cut(s) 122, 829
CviAII CATG 1 cut(s) 977
DdeI CTNAG 1 cut(s) 619
DinI GGCGCC 1 cut(s) 1044
DpnI GATC 6 cut(s) 302, 470, 507, 835, 939, 953
DpnII GATC 6 cut(s) 300, 468, 505, 833, 937, 951
DraI TTTAAA 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 3 cut(s) 264, 572, 599
EarI CTCTTC 3 cut(s) 264, 572, 599
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 2 cut(s) 669, 970
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 32
EcoT38I GRGCYC 1 cut(s) 34
EgeI GGCGCC 1 cut(s) 1044
EheI GGCGCC 1 cut(s) 1044
Esp3I CGTCTC 2 cut(s) 261, 596
FaeI CATG 1 cut(s) 980
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 2 cut(s) 330, 764
FatI CATG 1 cut(s) 976
FauI CCCGC 1 cut(s) 740
Fnu4HI GCNGC 4 cut(s) 390, 536, 632, 1122
FokI GGATG 2 cut(s) 228, 1103
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 4 cut(s) 390, 536, 632, 1122
FspBI CTAG 6 cut(s) 5, 68, 521, 942, 1023, 1194
GlaI GCGC 2 cut(s) 732, 1044
GluI GCNGC 4 cut(s) 390, 536, 632, 1122
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 1 cut(s) 671
HapII CCGG 6 cut(s) 314, 734, 841, 859, 1040, 1046
HgaI GACGC 2 cut(s) 122, 829
HhaI GCGC 2 cut(s) 733, 1045
Hin1I GRCGYC 3 cut(s) 821, 899, 1043
Hin1II CATG 1 cut(s) 980
Hin6I GCGC 2 cut(s) 731, 1043
HinP1I GCGC 2 cut(s) 731, 1043
HinfI GANTC 7 cut(s) 48, 234, 322, 624, 638, 680, 880
HpaII CCGG 6 cut(s) 314, 734, 841, 859, 1040, 1046
HphI GGTGA 7 cut(s) 537, 595, 701, 902, 1021, 1070, 1163
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 6 cut(s) 352, 367, 380, 679, 713, 989
Hpy188III TCNNGA 7 cut(s) 35, 157, 621, 758, 977, 1023, 1229
Hpy8I GTNNAC 1 cut(s) 652
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 6 cut(s) 266, 543, 549, 555, 755, 1055
HpyCH4IV ACGT 5 cut(s) 589, 726, 899, 957, 1135
HpyCH4V TGCA 3 cut(s) 346, 615, 1124
HpyF3I CTNAG 1 cut(s) 619
HpySE526I ACGT 5 cut(s) 589, 726, 899, 957, 1135
Hsp92I GRCGYC 3 cut(s) 821, 899, 1043
Hsp92II CATG 1 cut(s) 980
HspAI GCGC 2 cut(s) 731, 1043
KasI GGCGCC 1 cut(s) 1042
Kzo9I GATC 6 cut(s) 300, 468, 505, 833, 937, 951
LmnI GCTCC 4 cut(s) 77, 394, 529, 815
Lsp1109I GCAGC 1 cut(s) 1108
MaeI CTAG 6 cut(s) 5, 68, 521, 942, 1023, 1194
MaeII ACGT 5 cut(s) 589, 726, 899, 957, 1135
MaeIII GTNAC 3 cut(s) 543, 549, 755
MalI GATC 6 cut(s) 302, 470, 507, 835, 939, 953
MboI GATC 6 cut(s) 300, 468, 505, 833, 937, 951
MboII GAAGA 9 cut(s) 172, 197, 251, 586, 589, 675, 933, 976, 1096
MflI RGATCY 1 cut(s) 937
MhlI GDGCHC 1 cut(s) 34
MluCI AATT 6 cut(s) 119, 328, 432, 700, 1179, 1249
MluI ACGCGT 1 cut(s) 202
Mly113I GGCGCC 1 cut(s) 1043
MlyI GAGTC 1 cut(s) 42
MmeI TCCRAC 3 cut(s) 390, 691, 909
MnlI CCTC 8 cut(s) 46, 255, 330, 712, 730, 824, 955, 983
MseI TTAA 7 cut(s) 131, 144, 227, 488, 1182, 1209, 1264
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 6 cut(s) 314, 734, 841, 859, 1040, 1046
MspR9I CCNGG 2 cut(s) 315, 1040
MvnI CGCG 3 cut(s) 105, 204, 389
NarI GGCGCC 1 cut(s) 1043
NciI CCSGG 2 cut(s) 315, 1040
NdeII GATC 6 cut(s) 300, 468, 505, 833, 937, 951
NlaIII CATG 1 cut(s) 980
NlaIV GGNNCC 2 cut(s) 1044, 1069
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 3 cut(s) 543, 549, 755
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 1 cut(s) 976
PcsI WCGNNNNNNNCGW 2 cut(s) 539, 765
PfeI GAWTC 6 cut(s) 234, 322, 624, 638, 680, 880
PfoI TCCNGGA 1 cut(s) 313
PkrI GCNGC 4 cut(s) 391, 537, 633, 1123
Ple19I CGATCG 1 cut(s) 303
PleI GAGTC 1 cut(s) 42
PluTI GGCGCC 1 cut(s) 1046
PpsI GAGTC 1 cut(s) 42
PsiI TTATAA 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 34
Psp1406I AACGTT 1 cut(s) 957
PspN4I GGNNCC 2 cut(s) 1044, 1069
PspXI VCTCGAGB 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 937
PvuI CGATCG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 34
SaqAI TTAA 7 cut(s) 131, 144, 227, 488, 1182, 1209, 1264
SatI GCNGC 4 cut(s) 390, 536, 632, 1122
Sau3AI GATC 6 cut(s) 300, 468, 505, 833, 937, 951
SchI GAGTC 1 cut(s) 42
ScrFI CCNGG 2 cut(s) 315, 1040
SduI GDGCHC 1 cut(s) 34
SfoI GGCGCC 1 cut(s) 1044
Sfr274I CTCGAG 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 50
SmlI CTYRAG 2 cut(s) 50, 143
SmoI CTYRAG 2 cut(s) 50, 143
SpeI ACTAGT 1 cut(s) 1193
Sse9I AATT 6 cut(s) 119, 328, 432, 700, 1179, 1249
SsiI CCGC 9 cut(s) 103, 389, 392, 536, 598, 632, 747, 875, 1028
SspDI GGCGCC 1 cut(s) 1042
SspMI CTAG 6 cut(s) 5, 68, 521, 942, 1023, 1194
SstI GAGCTC 1 cut(s) 34
StyD4I CCNGG 2 cut(s) 313, 1038
TaaI ACNGT 6 cut(s) 266, 543, 549, 555, 755, 1055
TaiI ACGT 5 cut(s) 592, 729, 902, 960, 1138
TaqI TCGA 9 cut(s) 46, 51, 156, 213, 237, 303, 504, 771, 836
TasI AATT 6 cut(s) 119, 328, 432, 700, 1179, 1249
TauI GCSGC 3 cut(s) 392, 538, 634
TfiI GAWTC 6 cut(s) 234, 322, 624, 638, 680, 880
Tru1I TTAA 7 cut(s) 131, 144, 227, 488, 1182, 1209, 1264
Tru9I TTAA 7 cut(s) 131, 144, 227, 488, 1182, 1209, 1264
TscAI CASTG 1 cut(s) 558
TseFI GTSAC 3 cut(s) 543, 549, 755
TseI GCWGC 1 cut(s) 1121
Tsp45I GTSAC 3 cut(s) 543, 549, 755
TspDTI ATGAA 2 cut(s) 590, 1190
TspGWI ACGGA 1 cut(s) 243
TspRI CASTG 1 cut(s) 558
Vha464I CTTAAG 1 cut(s) 143
XapI RAATTY 1 cut(s) 700
XbaI TCTAGA 1 cut(s) 1022
XhoI CTCGAG 1 cut(s) 50
XspI CTAG 6 cut(s) 5, 68, 521, 942, 1023, 1194
ZraI GACGTC 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.