Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0010975

Physical Location & Seq

Genomic Coordinates Forward (+)

24938069 .. 24943072

Gene Structure 5004 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00185540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGAAAGGTAGGAAACAAGAGTCACTACCTAGAGATTGGAAAATGTCTTCACTTCTTTTCAAACCTAAACTAAAGGTGTCGTCGTTACACAAACTCCGGTCATTGGAAAGCATTCAATCAATCCGCTCCCAGACAAGACTCCGAAGCCGGCTCTCAGAACTTCTCTATAAAACCGCCCACAACTCCAGAACCTCCCAAATTTCTGGTAACCCCAAGTCGTCCTCTACCATGGATCAAGCAGAACCTCAGCGCTGCGGCGACGACGCCGTTTCGGTGCCTAGGTTCAATTTCCAGCGCCTTAAAGTCATCTCGCCGGTCGGCCGCGGAGCCAAGGGAGTCGTGTTTTTGGTGAAAGACCGAGAGACGGAGGAGCTCATGGCCTTGAAGGTAATTTCCAAGGACTTGATCCACCGCAAGGGCAAGGACGGCGACGGCGACGAGTACCGCCGAGTCTGCTTCGAGCAGCAAGTACTACGTCGTTTTGATCACCCGCTCCTCCCGAAACTGCATGGAGTCCTCGACACTGAGAAGCTCGTCGGTTACGCCATGGATTACTGCTCCGGCCGCGATCTCAATTCCCTCCGGAAGAGGCAGACGGAGAGGATGTTCTCCGATGACGTCATCAGATTTTACGCGGCGGAATTGGTTCTGGCATTGGAGTATTTGCACGGTTTAGGAGTGGTTTACCGGGATTTGAAGCCGGAAAACGTTATGATCCAAGGAAACGGCCACATTATGCTCGTGGATTTCGATCTTTCAACCAAACTCTCGCCGCCAATCAAGACTCCCCAACCAGCTCCGAGATCGAATTCCGTATCCAAATCCGCTTCCGTCCACAAGAAGAACAAGACGAAGAAGAAGCGCTCGTCACTCTTCCACAGCTTCTGCAATTCGGGAATTTCGCCGGACGATTTGATCGCACCGCCAGAAACCGAAACCGATGCGAATCCGGATGACAAAACCGATTCGGACTCGTCCGGGAAGTCGAACTCGTTCGTCGGAACGGAGGAGTACGTGGCGCCGGAGATCATATCCGCCAAAGGCCACGGCTTCGAAGTCGACTGGTGGTCCCTGGGAGTCTTACTATACGAAATGCTGTACGGTACGACGCCGTTCAGAGGCTCCAACCGGAAGGAGACGTTTTACCGGATACTGACCAAGGCCCCGGAGCTGACGGGCGAAGCGACGCCGTTAAGGGACCTGATCAAGAACCTGCTGGAGAAGGATCCGAAGCAGAGAATCGGGTTGGATGAAATCAAGGGCCACGAGTTTTTCAGAGGAATCGGCGGACGGTGGGATTCGGTGGCGCAACTCCCGAGGCCGCCGTATATTCCCGAGGAGGTGGCCGAGAGTACGGATGGCACTAAGAAAATAGACGTGGAGTCCGTGGTGGAGGGAATATTCGGAAATGGCGGTGGAGAGGAGGAGAATAAGAATAACCATAAATATGTGGAGGAGGAGAATGTGAATAAAAGGGTGTGGAAGGAAGAACTTGATCACCACCACCCCACTCAACCTGATGCCTTCTCCGTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

512

Amino Acids

57.94

Weight (kDa)

8.7

Isoelectric Point (pI)

47.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	101 - 256	1.8e-13	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	102 - 260	2.1e-32	Protein kinase domain
Pkinase	PF00069	325 - 425	5.8e-24	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	330 - 420	7.7e-08	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25250 AT4G13000
fragaria_vesca	FvH4_2g18040
malus_domestica	MD05G1164900.v1.1 MD10G1154200.v1.1
prunus_persica	Prupe.8G193100_v2.0.a1
pyrus_communis	pycom05g15350 pycom10g13330
rosa_chinensis	RchiOBHm_Chr6g0283431
rosa_laevigata	RLG00000012827
rosa_multiflora	Rmu_sc0003166.1_g000017 Rmu_sc0004959.1_g000020 Rmu_sc0039373.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185540
rosa_rugosa	Rorug06G0154100
rosa_samantha	Rh6AG266400 Rh6BG268900 Rh6CG268800 Rh6DG262200
rosa_wichuraiana	Rw6G023060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	621
Acc36I	ACCTGC	1 cut(s)	1221
AccB1I	GGYRCC	2 cut(s)	274, 1018
AccBSI	CCGCTC	2 cut(s)	126, 493
AccI	GTMKAC	1 cut(s)	1059
AccII	CGCG	3 cut(s)	324, 567, 635
AccIII	TCCGGA	2 cut(s)	582, 949
AclI	AACGTT	1 cut(s)	708
AclWI	GGATC	5 cut(s)	240, 400, 709, 1220, 1233
AcoI	YGGCCR	4 cut(s)	319, 562, 727, 1344
AcsI	RAATTY	3 cut(s)	198, 808, 897
AcyI	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
AfaI	GTAC	6 cut(s)	443, 471, 1013, 1100, 1105, 1354
AfeI	AGCGCT	2 cut(s)	251, 863
AfiI	CCNNNNNNNGG	4 cut(s)	103, 364, 415, 1118
AgsI	TTSAA	6 cut(s)	61, 116, 286, 385, 697, 759
AhdI	GACNNNNNGTC	2 cut(s)	1066, 1381
AjiI	CACGTC	1 cut(s)	1378
AjnI	CCWGG	1 cut(s)	1071
AloI	GAACNNNNNNTCC	2 cut(s)	590, 622
AluBI	AGCT	5 cut(s)	373, 532, 797, 882, 1171
AluI	AGCT	5 cut(s)	373, 532, 797, 882, 1171
Alw21I	GWGCWC	1 cut(s)	375
Alw26I	GTCTC	2 cut(s)	356, 1130
AlwI	GGATC	5 cut(s)	240, 400, 709, 1220, 1233
AlwNI	CAGNNNCTG	1 cut(s)	885
Ama87I	CYCGRG	2 cut(s)	1315, 1334
Aor13HI	TCCGGA	2 cut(s)	582, 949
Aor51HI	AGCGCT	2 cut(s)	251, 863
AoxI	GGCC	9 cut(s)	319, 378, 562, 727, 1042, 1161, 1261, 1319, 1344
ApeKI	GCWGC	2 cut(s)	252, 463
ApoI	RAATTY	3 cut(s)	198, 808, 897
Asp700I	GAANNNNTTC	3 cut(s)	111, 645, 992
AspA2I	CCTAGG	1 cut(s)	278
AspLEI	GCGC	5 cut(s)	252, 297, 864, 1021, 1309
AspS9I	GGNCC	4 cut(s)	1068, 1162, 1198, 1261
AsuC2I	CCSGG	3 cut(s)	689, 979, 1166
AsuHPI	GGTGA	3 cut(s)	361, 479, 1490
AsuII	TTCGAA	1 cut(s)	1053
AvaI	CYCGRG	2 cut(s)	1315, 1334
AvaII	GGWCC	2 cut(s)	1068, 1198
AvrII	CCTAGG	1 cut(s)	278
BamHI	GGATCC	1 cut(s)	1225
BanI	GGYRCC	2 cut(s)	274, 1018
BanII	GRGCYC	1 cut(s)	375
BauI	CACGAG	2 cut(s)	740, 1265
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	1 cut(s)	375
BbvCI	CCTCAGC	1 cut(s)	246
BbvI	GCAGC	2 cut(s)	239, 475
BccI	CCATC	1 cut(s)	1352
BceAI	ACGGC	8 cut(s)	251, 442, 448, 742, 1063, 1096, 1174, 1309
BcgI	CGANNNNNNTGC	2 cut(s)	923, 957
BciT130I	CCWGG	1 cut(s)	1073
BciVI	GTATCC	2 cut(s)	826, 1143
BclI	TGATCA	3 cut(s)	484, 1203, 1495
BcnI	CCSGG	3 cut(s)	689, 979, 1166
BcoDI	GTCTC	2 cut(s)	356, 1130
BfaI	CTAG	2 cut(s)	30, 279
BfoI	RGCGCY	4 cut(s)	253, 298, 865, 1022
BfuAI	ACCTGC	1 cut(s)	1221
BfuI	GTATCC	2 cut(s)	826, 1143
BisI	GCNGC	8 cut(s)	253, 256, 322, 464, 565, 636, 773, 1322
BlnI	CCTAGG	1 cut(s)	278
BlsI	GCNGC	8 cut(s)	254, 257, 323, 465, 566, 637, 774, 1323
BmcAI	AGTACT	1 cut(s)	471
Bme1390I	CCNGG	4 cut(s)	689, 979, 1073, 1166
Bme18I	GGWCC	2 cut(s)	1068, 1198
BmeRI	GACNNNNNGTC	2 cut(s)	1066, 1381
BmeT110I	CYCGRG	2 cut(s)	1315, 1334
BmgBI	CACGTC	1 cut(s)	1378
BmgT120I	GGNCC	4 cut(s)	1068, 1162, 1198, 1261
BmiI	GGNNCC	8 cut(s)	276, 328, 1020, 1070, 1123, 1164, 1199, 1227
BmrFI	CCNGG	4 cut(s)	689, 979, 1073, 1166
BmsI	GCATC	2 cut(s)	931, 1510
BpiI	GAAGAC	1 cut(s)	39
BpmI	CTGGAG	2 cut(s)	169, 1238
Bpu10I	CCTNAGC	1 cut(s)	246
Bpu14I	TTCGAA	1 cut(s)	1053
BpuMI	CCSGG	3 cut(s)	689, 979, 1166
BsaAI	YACGTR	1 cut(s)	1015
BsaBI	GATNNNNATC	2 cut(s)	750, 945
BsaHI	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
BsaWI	WCCGGW	5 cut(s)	96, 582, 949, 1128, 1146
BsaXI	ACNNNNNCTCC	4 cut(s)	78, 108, 590, 620
Bsc4I	CCNNNNNNNGG	4 cut(s)	103, 364, 415, 1118
Bse118I	RCCGGY	2 cut(s)	147, 313
Bse1I	ACTGG	1 cut(s)	1067
Bse8I	GATNNNNATC	2 cut(s)	750, 945
BseAI	TCCGGA	2 cut(s)	582, 949
BseBI	CCWGG	1 cut(s)	1073
BseGI	GGATG	4 cut(s)	609, 958, 1255, 1363
BseJI	GATNNNNATC	2 cut(s)	750, 945
BseLI	CCNNNNNNNGG	4 cut(s)	103, 364, 415, 1118
BseMII	CTCAG	3 cut(s)	168, 260, 516
BseNI	ACTGG	1 cut(s)	1067
BseRI	GAGGAG	8 cut(s)	383, 485, 1022, 1352, 1436, 1439, 1469, 1472
BseX3I	CGGCCG	2 cut(s)	319, 562
BseXI	GCAGC	2 cut(s)	239, 475
Bsh1236I	CGCG	3 cut(s)	324, 567, 635
Bsh1285I	CGRYCG	3 cut(s)	318, 322, 565
BshFI	GGCC	9 cut(s)	321, 380, 564, 729, 1044, 1163, 1263, 1321, 1346
BshNI	GGYRCC	2 cut(s)	274, 1018
BsiEI	CGRYCG	3 cut(s)	318, 322, 565
BsiHKAI	GWGCWC	1 cut(s)	375
BsiHKCI	CYCGRG	2 cut(s)	1315, 1334
BslFI	GGGAC	2 cut(s)	1054, 1211
BslI	CCNNNNNNNGG	4 cut(s)	103, 364, 415, 1118
BsmAI	GTCTC	2 cut(s)	356, 1130
BsmBI	CGTCTC	2 cut(s)	356, 1130
BsmFI	GGGAC	2 cut(s)	1054, 1211
BsmI	GAATGC	1 cut(s)	111
BsnI	GGCC	9 cut(s)	321, 380, 564, 729, 1044, 1163, 1263, 1321, 1346
BsoBI	CYCGRG	2 cut(s)	1315, 1334
Bsp119I	TTCGAA	1 cut(s)	1053
Bsp1286I	GDGCHC	1 cut(s)	375
Bsp13I	TCCGGA	2 cut(s)	582, 949
Bsp19I	CCATGG	2 cut(s)	228, 546
BspANI	GGCC	9 cut(s)	321, 380, 564, 729, 1044, 1163, 1263, 1321, 1346
BspCNI	CTCAG	3 cut(s)	167, 259, 517
BspEI	TCCGGA	2 cut(s)	582, 949
BspFNI	CGCG	3 cut(s)	324, 567, 635
BspLI	GGNNCC	8 cut(s)	276, 328, 1020, 1070, 1123, 1164, 1199, 1227
BspMI	ACCTGC	1 cut(s)	1221
BspPI	GGATC	5 cut(s)	240, 400, 709, 1220, 1233
BspT104I	TTCGAA	1 cut(s)	1053
BspT107I	GGYRCC	2 cut(s)	274, 1018
BsrBI	CCGCTC	2 cut(s)	126, 493
BsrFI	RCCGGY	2 cut(s)	147, 313
BsrI	ACTGG	1 cut(s)	1067
BssAI	RCCGGY	2 cut(s)	147, 313
BssNI	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
BssSI	CACGAG	2 cut(s)	740, 1265
BssT1I	CCWWGG	7 cut(s)	228, 278, 330, 396, 546, 718, 1158
Bst2BI	CACGAG	2 cut(s)	740, 1265
Bst2UI	CCWGG	1 cut(s)	1073
Bst4CI	ACNGT	3 cut(s)	671, 1103, 1293
Bst6I	CTCTTC	2 cut(s)	581, 878
BstACI	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
BstBAI	YACGTR	1 cut(s)	1015
BstBI	TTCGAA	1 cut(s)	1053
BstC8I	GCNNGC	1 cut(s)	149
BstDEI	CTNAG	4 cut(s)	154, 246, 525, 1365
BstDSI	CCRYGG	5 cut(s)	228, 322, 546, 1045, 1386
BstEII	GGTNACC	1 cut(s)	206
BstF5I	GGATG	4 cut(s)	609, 958, 1255, 1363
BstFNI	CGCG	3 cut(s)	324, 567, 635
BstH2I	RGCGCY	4 cut(s)	253, 298, 865, 1022
BstHHI	GCGC	5 cut(s)	252, 297, 864, 1021, 1309
BstMAI	GTCTC	2 cut(s)	356, 1130
BstMCI	CGRYCG	3 cut(s)	318, 322, 565
BstMWI	GCNNNNNNNGC	3 cut(s)	426, 453, 564
BstNI	CCWGG	1 cut(s)	1073
BstPI	GGTNACC	1 cut(s)	206
BstSCI	CCNGG	4 cut(s)	687, 977, 1071, 1164
BstUI	CGCG	3 cut(s)	324, 567, 635
BstV1I	GCAGC	2 cut(s)	239, 475
BstV2I	GAAGAC	1 cut(s)	39
BstX2I	RGATCY	1 cut(s)	1225
BstXI	CCANNNNNNTGG	1 cut(s)	203
BstYI	RGATCY	1 cut(s)	1225
BstZI	CGGCCG	2 cut(s)	319, 562
BsuI	GTATCC	2 cut(s)	826, 1143
BsuRI	GGCC	9 cut(s)	321, 380, 564, 729, 1044, 1163, 1263, 1321, 1346
BtgI	CCRYGG	5 cut(s)	228, 322, 546, 1045, 1386
BtrI	CACGTC	1 cut(s)	1378
BtsCI	GGATG	4 cut(s)	609, 958, 1255, 1363
BtsIMutI	CAGTG	1 cut(s)	522
BveI	ACCTGC	1 cut(s)	1221
Cac8I	GCNNGC	1 cut(s)	149
CaiI	CAGNNNCTG	1 cut(s)	885
CfoI	GCGC	5 cut(s)	252, 297, 864, 1021, 1309
Cfr10I	RCCGGY	2 cut(s)	147, 313
Cfr13I	GGNCC	4 cut(s)	1068, 1162, 1198, 1261
Cfr42I	CCGCGG	1 cut(s)	325
CseI	GACGC	3 cut(s)	272, 1117, 1195
Csp6I	GTAC	6 cut(s)	442, 470, 1012, 1099, 1104, 1353
CviAII	CATG	4 cut(s)	229, 376, 509, 547
CviQI	GTAC	6 cut(s)	442, 470, 1012, 1099, 1104, 1353
DdeI	CTNAG	4 cut(s)	154, 246, 525, 1365
DinI	GGCGCC	1 cut(s)	1020
DriI	GACNNNNNGTC	2 cut(s)	1066, 1381
EaeI	YGGCCR	4 cut(s)	319, 562, 727, 1344
EagI	CGGCCG	2 cut(s)	319, 562
Eam1104I	CTCTTC	2 cut(s)	581, 878
Eam1105I	GACNNNNNGTC	2 cut(s)	1066, 1381
EarI	CTCTTC	2 cut(s)	581, 878
EciI	GGCGGA	3 cut(s)	653, 1024, 1302
Ecl136II	GAGCTC	1 cut(s)	373
EclXI	CGGCCG	2 cut(s)	319, 562
Eco130I	CCWWGG	7 cut(s)	228, 278, 330, 396, 546, 718, 1158
Eco24I	GRGCYC	1 cut(s)	375
Eco47I	GGWCC	2 cut(s)	1068, 1198
Eco47III	AGCGCT	2 cut(s)	251, 863
Eco52I	CGGCCG	2 cut(s)	319, 562
Eco53kI	GAGCTC	1 cut(s)	373
Eco88I	CYCGRG	2 cut(s)	1315, 1334
Eco91I	GGTNACC	1 cut(s)	206
EcoICRI	GAGCTC	1 cut(s)	373
EcoO109I	RGGNCCY	2 cut(s)	1162, 1198
EcoO65I	GGTNACC	1 cut(s)	206
EcoRI	GAATTC	1 cut(s)	808
EcoRII	CCWGG	1 cut(s)	1071
EcoT14I	CCWWGG	7 cut(s)	228, 278, 330, 396, 546, 718, 1158
EcoT38I	GRGCYC	1 cut(s)	375
EgeI	GGCGCC	1 cut(s)	1020
EheI	GGCGCC	1 cut(s)	1020
ErhI	CCWWGG	7 cut(s)	228, 278, 330, 396, 546, 718, 1158
Esp3I	CGTCTC	2 cut(s)	356, 1130
FaeI	CATG	4 cut(s)	232, 379, 512, 550
FaqI	GGGAC	2 cut(s)	1054, 1211
FatI	CATG	4 cut(s)	228, 375, 508, 546
FauI	CCCGC	1 cut(s)	498
FbaI	TGATCA	3 cut(s)	484, 1203, 1495
FblI	GTMKAC	1 cut(s)	1059
Fnu4HI	GCNGC	8 cut(s)	253, 256, 322, 464, 565, 636, 773, 1322
FokI	GGATG	4 cut(s)	616, 965, 1262, 1370
FriOI	GRGCYC	1 cut(s)	375
Fsp4HI	GCNGC	8 cut(s)	253, 256, 322, 464, 565, 636, 773, 1322
FspBI	CTAG	2 cut(s)	30, 279
GlaI	GCGC	5 cut(s)	251, 296, 863, 1020, 1308
GluI	GCNGC	8 cut(s)	253, 256, 322, 464, 565, 636, 773, 1322
GsuI	CTGGAG	2 cut(s)	169, 1238
HaeII	RGCGCY	4 cut(s)	253, 298, 865, 1022
HaeIII	GGCC	9 cut(s)	321, 380, 564, 729, 1044, 1163, 1263, 1321, 1346
HgaI	GACGC	3 cut(s)	272, 1117, 1195
HhaI	GCGC	5 cut(s)	252, 297, 864, 1021, 1309
Hin1I	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
Hin1II	CATG	4 cut(s)	232, 379, 512, 550
Hin6I	GCGC	5 cut(s)	250, 295, 862, 1019, 1307
HinP1I	GCGC	5 cut(s)	250, 295, 862, 1019, 1307
HincII	GTYRAC	1 cut(s)	1060
HindII	GTYRAC	1 cut(s)	1060
HphI	GGTGA	3 cut(s)	361, 479, 1490
Hpy166II	GTNNAC	3 cut(s)	685, 835, 1060
Hpy188III	TCNNGA	9 cut(s)	186, 499, 583, 781, 894, 950, 1207, 1315, 1334
Hpy8I	GTNNAC	3 cut(s)	685, 835, 1060
HpyAV	CCTTC	5 cut(s)	379, 1126, 1216, 1477, 1534
HpyCH4III	ACNGT	3 cut(s)	671, 1103, 1293
HpyCH4IV	ACGT	6 cut(s)	475, 618, 708, 1014, 1139, 1377
HpyCH4V	TGCA	3 cut(s)	508, 667, 888
HpyF10VI	GCNNNNNNNGC	3 cut(s)	426, 453, 564
HpyF3I	CTNAG	4 cut(s)	154, 246, 525, 1365
HpySE526I	ACGT	6 cut(s)	475, 618, 708, 1014, 1139, 1377
Hsp92I	GRCGYC	5 cut(s)	264, 618, 1019, 1109, 1187
Hsp92II	CATG	4 cut(s)	232, 379, 512, 550
HspAI	GCGC	5 cut(s)	250, 295, 862, 1019, 1307
KasI	GGCGCC	1 cut(s)	1018
Kpn2I	TCCGGA	2 cut(s)	582, 949
KroI	GCCGGC	1 cut(s)	147
KroNI	GCCGGC	1 cut(s)	149
Ksp22I	TGATCA	3 cut(s)	484, 1203, 1495
KspI	CCGCGG	1 cut(s)	325
LmnI	GCTCC	8 cut(s)	131, 326, 370, 498, 563, 802, 1127, 1168
Lsp1109I	GCAGC	2 cut(s)	239, 475
LweI	GCATC	2 cut(s)	931, 1510
MaeI	CTAG	2 cut(s)	30, 279
MaeII	ACGT	6 cut(s)	475, 618, 708, 1014, 1139, 1377
MaeIII	GTNAC	5 cut(s)	21, 84, 206, 539, 867
MbiI	CCGCTC	2 cut(s)	126, 493
MboII	GAAGA	7 cut(s)	39, 598, 853, 865, 865, 868, 1499
MflI	RGATCY	1 cut(s)	1225
MhlI	GDGCHC	1 cut(s)	375
MluCI	AATT	8 cut(s)	198, 286, 390, 574, 641, 808, 889, 897
Mly113I	GGCGCC	1 cut(s)	1019
MlyI	GAGTC	9 cut(s)	29, 132, 345, 459, 522, 778, 965, 1086, 1391
MmeI	TCCRAC	3 cut(s)	979, 1149, 1227
MroI	TCCGGA	2 cut(s)	582, 949
MroNI	GCCGGC	1 cut(s)	147
MroXI	GAANNNNTTC	3 cut(s)	111, 645, 992
MseI	TTAA	2 cut(s)	300, 1193
MslI	CAYNNNNRTG	1 cut(s)	1446
MspA1I	CMGCKG	1 cut(s)	324
MspR9I	CCNGG	4 cut(s)	689, 979, 1073, 1166
Mva1269I	GAATGC	1 cut(s)	111
MvaI	CCWGG	1 cut(s)	1073
MvnI	CGCG	3 cut(s)	324, 567, 635
MwoI	GCNNNNNNNGC	3 cut(s)	426, 453, 564
NaeI	GCCGGC	1 cut(s)	149
NarI	GGCGCC	1 cut(s)	1019
NciI	CCSGG	3 cut(s)	689, 979, 1166
NcoI	CCATGG	2 cut(s)	228, 546
NgoMIV	GCCGGC	1 cut(s)	147
NlaIII	CATG	4 cut(s)	232, 379, 512, 550
NlaIV	GGNNCC	8 cut(s)	276, 328, 1020, 1070, 1123, 1164, 1199, 1227
NmeAIII	GCCGAG	2 cut(s)	473, 1372
NmuCI	GTSAC	2 cut(s)	21, 867
NspV	TTCGAA	1 cut(s)	1053
PasI	CCCWGGG	1 cut(s)	1072
PcsI	WCGNNNNNNNCGW	4 cut(s)	432, 747, 1181, 1383
PctI	GAATGC	1 cut(s)	111
PdiI	GCCGGC	1 cut(s)	149
PdmI	GAANNNNTTC	3 cut(s)	111, 645, 992
PfeI	GAWTC	5 cut(s)	946, 965, 1239, 1281, 1298
PfoI	TCCNGGA	1 cut(s)	977
PkrI	GCNGC	8 cut(s)	254, 257, 323, 465, 566, 637, 774, 1323
PleI	GAGTC	9 cut(s)	28, 132, 344, 458, 521, 778, 965, 1085, 1390
PluTI	GGCGCC	1 cut(s)	1022
PpsI	GAGTC	9 cut(s)	28, 132, 344, 458, 521, 778, 965, 1085, 1390
Ppu21I	YACGTR	1 cut(s)	1015
PpuMI	RGGWCCY	1 cut(s)	1198
Psp124BI	GAGCTC	1 cut(s)	375
Psp1406I	AACGTT	1 cut(s)	708
Psp5II	RGGWCCY	1 cut(s)	1198
Psp6I	CCWGG	1 cut(s)	1071
PspEI	GGTNACC	1 cut(s)	206
PspGI	CCWGG	1 cut(s)	1071
PspN4I	GGNNCC	8 cut(s)	276, 328, 1020, 1070, 1123, 1164, 1199, 1227
PspPI	GGNCC	4 cut(s)	1068, 1162, 1198, 1261
PspPPI	RGGWCCY	1 cut(s)	1198
PstNI	CAGNNNCTG	1 cut(s)	885
PsuI	RGATCY	1 cut(s)	1225
RsaI	GTAC	6 cut(s)	443, 471, 1013, 1100, 1105, 1354
RsaNI	GTAC	6 cut(s)	442, 470, 1012, 1099, 1104, 1353
RseI	CAYNNNNRTG	1 cut(s)	1446
SacI	GAGCTC	1 cut(s)	375
SacII	CCGCGG	1 cut(s)	325
SalI	GTCGAC	1 cut(s)	1058
SaqAI	TTAA	2 cut(s)	300, 1193
SatI	GCNGC	8 cut(s)	253, 256, 322, 464, 565, 636, 773, 1322
Sau96I	GGNCC	4 cut(s)	1068, 1162, 1198, 1261
ScaI	AGTACT	1 cut(s)	471
SchI	GAGTC	9 cut(s)	29, 132, 345, 459, 522, 778, 965, 1086, 1391
ScrFI	CCNGG	4 cut(s)	689, 979, 1073, 1166
SduI	GDGCHC	1 cut(s)	375
SfaNI	GCATC	2 cut(s)	931, 1510
SfoI	GGCGCC	1 cut(s)	1020
Sfr303I	CCGCGG	1 cut(s)	325
SfuI	TTCGAA	1 cut(s)	1053
SgrBI	CCGCGG	1 cut(s)	325
SinI	GGWCC	2 cut(s)	1068, 1198
SmiMI	CAYNNNNRTG	1 cut(s)	1446
Sse9I	AATT	8 cut(s)	198, 286, 390, 574, 641, 808, 889, 897
SspDI	GGCGCC	1 cut(s)	1018
SspI	AATATT	1 cut(s)	1401
SspMI	CTAG	2 cut(s)	30, 279
SstI	GAGCTC	1 cut(s)	375
StyD4I	CCNGG	4 cut(s)	687, 977, 1071, 1164
StyI	CCWWGG	7 cut(s)	228, 278, 330, 396, 546, 718, 1158
TaaI	ACNGT	3 cut(s)	671, 1103, 1293
TaiI	ACGT	6 cut(s)	478, 621, 711, 1017, 1142, 1380
TaqI	TCGA	7 cut(s)	459, 519, 750, 806, 986, 1053, 1059
TaqII	GACCGA	1 cut(s)	372
TasI	AATT	8 cut(s)	198, 286, 390, 574, 641, 808, 889, 897
TatI	WGTACW	1 cut(s)	469
TauI	GCSGC	6 cut(s)	258, 324, 567, 638, 775, 1324
TfiI	GAWTC	5 cut(s)	946, 965, 1239, 1281, 1298
Tru1I	TTAA	2 cut(s)	300, 1193
Tru9I	TTAA	2 cut(s)	300, 1193
TscAI	CASTG	1 cut(s)	529
TseFI	GTSAC	2 cut(s)	21, 867
TseI	GCWGC	2 cut(s)	252, 463
Tsp45I	GTSAC	2 cut(s)	21, 867
TspDTI	ATGAA	2 cut(s)	17, 1266
TspGWI	ACGGA	8 cut(s)	380, 611, 802, 820, 1019, 1370, 1375, 1519
TspRI	CASTG	1 cut(s)	529
VpaK11BI	GGWCC	2 cut(s)	1068, 1198
XapI	RAATTY	3 cut(s)	198, 808, 897
XmaJI	CCTAGG	1 cut(s)	278
XmiI	GTMKAC	1 cut(s)	1059
XmnI	GAANNNNTTC	3 cut(s)	111, 645, 992
XspI	CTAG	2 cut(s)	30, 279
ZraI	GACGTC	1 cut(s)	619
ZrmI	AGTACT	1 cut(s)	471

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.