Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010975

Physical Location & Seq

Genomic Coordinates Reverse (-)

24193421 .. 24194910

Gene Structure 1490 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1154200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGCCACCCGAAACAGAGCTTTCAACCATGGCCGACCAAGACGACGGCGTCACGGTTCCCAGCTTGGATTTCCAGCGCCTGAGAGTGATCTCGGTGCTGGGCCGCGGAGCCAAGGGCGTGGTGTTTCTGGTGAAGGGCGACGAGGCGGCAGAGGAGCTCATGGCCTTAAAGGTAATTTCCAAGGACTTGATCGAGCGGAGGAGTAAAGACGCGAAGAGCGACGGCAGCGAGTACCGAAGAGTCAGCTTCGAGCAGCAAGTACTACGTCGTTTTGACCACCCGCTCTTGCCGAAGCTGCATGGCGTTCTCGACACTGAAAAGCTCGCCGGCTACGCCATTGATTACTGTCCCGGCCGCGATCTCAATTGCCTCAGAAAACGACAGACCGAGCGGATGTTCTCCGATGAGGTCATCAGATTTTACGCGGCGGAATTGGTTCTGGTATTGGATTATCTGCACGGATTAGGAGTGGTTTACAGAGATTTGAAACCGGAGAACATCATGATCCAAGAGAACGGTCACATTATGCTCGTCGATTTCGATCTCTCCACCGAGCTCTCTCCGATAAGGACTCCTCCTCGATCAGCTCAATCAGCTCAATCGGATCGGATTATATCAAATTCCATAGCAAAATCAATCCCAGTGCAAAAGAAGCGGCGCTCGCCGTTCCACCGCTTCTGCAACTCGGGGATTTCGCCGGACGAAACGGTGGCGCCGCCCGAACTCCGCGTCAACTCGGAAAGCCCCTCCGGTTCGGATTTGTCCGAGAAGTCGAACTCGTTTGTTGGGACGGAGGAGTACGTGGCGCCCGAAATTGTATCGGGCCATGGCCATGACTTCGGCGTCGATTGGTGGTCCCTCGGGGTGCTTCTTTACGAAATGCTGTACGGTACGACACCGTTCCGGGGATCTAACAGAAAAGAGACGTTTTATCGGATACTGAAAAAAACCCCTGAACTAAGAGGCCAAACGACGGCTTTAAGGGACCTGATAAAAAATTTGCTCGAGAAGGATCCGAAGCAGAGAATCGGGTCGGTGGAGATCAAGGGCCACGATTTCTTTAAAGAGGTTGAGTGGGATTCGGTTTTGCGAATCTCGAGGCCGCCGTACATTCCCGAAATTGCGCCGGCGGAGGTTACGGACGGAATTGAAAAGAAAATTGATGTGGAGACGGTGGTGAAAGGAATATTCGGTGGTGAAGATGGTGGTGGGGAGGAGGAGGAGAATAAAGGCAAGGAAAGTGAGGAGAATAATAGAAAAAGGAATAATAATGGGGAGGAAGGAAATAAGGAGGATGTAAATAAAAGGGTTTGTGTGGAAGGATTGAATTTGAATGACAACCCCACCCAAGATTGTGATGCATTTTTGGTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

459

Amino Acids

51.57

Weight (kDa)

5.24

Isoelectric Point (pI)

53.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	28 - 186	1.2e-11	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	29 - 188	6.9e-28	Protein kinase domain
Pkinase	PF00069	256 - 354	3.3e-23	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	265 - 344	4.9e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010975)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25250 AT4G13000
fragaria_vesca	FvH4_2g18040
malus_domestica	MD05G1164900.v1.1 MD10G1154200.v1.1
prunus_persica	Prupe.8G193100_v2.0.a1
pyrus_communis	pycom05g15350 pycom10g13330
rosa_chinensis	RchiOBHm_Chr6g0283431
rosa_laevigata	RLG00000012827
rosa_multiflora	Rmu_sc0003166.1_g000017 Rmu_sc0004959.1_g000020 Rmu_sc0039373.1_g000001
rosa_roxburghii	Rroxscaffold_7G00185540
rosa_rugosa	Rorug06G0154100
rosa_samantha	Rh6AG266400 Rh6BG268900 Rh6CG268800 Rh6DG262200
rosa_wichuraiana	Rw6G023060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	47, 842
AccB1I	GGYRCC	2 cut(s)	712, 805
AccBSI	CCGCTC	3 cut(s)	196, 283, 391
AccII	CGCG	5 cut(s)	105, 212, 357, 425, 729
AclWI	GGATC	5 cut(s)	499, 612, 916, 1007, 1020
AcoI	YGGCCR	3 cut(s)	30, 352, 829
AcsI	RAATTY	3 cut(s)	619, 997, 1327
AcyI	GRCGYC	4 cut(s)	48, 713, 806, 843
AfaI	GTAC	6 cut(s)	233, 261, 800, 887, 892, 1109
AfiI	CCNNNNNNNGG	2 cut(s)	905, 973
AgsI	TTSAA	5 cut(s)	24, 487, 1151, 1327, 1333
AluBI	AGCT	9 cut(s)	19, 63, 157, 246, 295, 322, 556, 587, 596
AluI	AGCT	9 cut(s)	19, 63, 157, 246, 295, 322, 556, 587, 596
Alw21I	GWGCWC	2 cut(s)	159, 558
Alw26I	GTCTC	2 cut(s)	917, 1163
AlwI	GGATC	5 cut(s)	499, 612, 916, 1007, 1020
AlwNI	CAGNNNCTG	1 cut(s)	79
Ama87I	CYCGRG	4 cut(s)	685, 860, 1004, 1096
AoxI	GGCC	9 cut(s)	30, 100, 162, 352, 823, 829, 964, 1048, 1100
ApeKI	GCWGC	3 cut(s)	225, 253, 295
ApoI	RAATTY	3 cut(s)	619, 997, 1327
ArsI	GACNNNNNNTTYG	2 cut(s)	714, 746
Asp700I	GAANNNNTTC	1 cut(s)	435
AspLEI	GCGC	5 cut(s)	78, 660, 715, 808, 1126
AspS9I	GGNCC	5 cut(s)	100, 823, 855, 985, 1048
AsuC2I	CCSGG	2 cut(s)	351, 905
AsuHPI	GGTGA	3 cut(s)	142, 1189, 1208
AvaI	CYCGRG	4 cut(s)	685, 860, 1004, 1096
AvaII	GGWCC	2 cut(s)	855, 985
BalI	TGGCCA	1 cut(s)	831
BamHI	GGATCC	1 cut(s)	1012
BanI	GGYRCC	2 cut(s)	712, 805
BanII	GRGCYC	2 cut(s)	159, 558
Bbv12I	GWGCWC	2 cut(s)	159, 558
BbvI	GCAGC	3 cut(s)	237, 265, 282
BccI	CCATC	1 cut(s)	1196
BceAI	ACGGC	5 cut(s)	61, 238, 649, 990, 1090
BciVI	GTATCC	1 cut(s)	930
BcnI	CCSGG	2 cut(s)	351, 905
BcoDI	GTCTC	2 cut(s)	917, 1163
BfoI	RGCGCY	4 cut(s)	79, 661, 716, 809
BfuI	GTATCC	1 cut(s)	930
BmcAI	AGTACT	1 cut(s)	261
Bme1390I	CCNGG	2 cut(s)	351, 905
Bme18I	GGWCC	2 cut(s)	855, 985
BmeT110I	CYCGRG	4 cut(s)	685, 860, 1004, 1096
BmgT120I	GGNCC	5 cut(s)	100, 823, 855, 985, 1048
BmiI	GGNNCC	7 cut(s)	57, 109, 714, 807, 857, 986, 1014
BmrFI	CCNGG	2 cut(s)	351, 905
BmrI	ACTGGG	1 cut(s)	635
BmsI	GCATC	1 cut(s)	1348
BmuI	ACTGGG	1 cut(s)	635
BpuMI	CCSGG	2 cut(s)	351, 905
BsaAI	YACGTR	1 cut(s)	802
BsaBI	GATNNNNATC	1 cut(s)	540
BsaHI	GRCGYC	4 cut(s)	48, 713, 806, 843
BsaJI	CCNNGG	7 cut(s)	27, 103, 111, 180, 826, 859, 904
BsaWI	WCCGGW	2 cut(s)	490, 749
Bsc4I	CCNNNNNNNGG	2 cut(s)	905, 973
Bse118I	RCCGGY	2 cut(s)	326, 1126
Bse1I	ACTGG	1 cut(s)	641
Bse8I	GATNNNNATC	1 cut(s)	540
BseDI	CCNNGG	7 cut(s)	27, 103, 111, 180, 826, 859, 904
BseGI	GGATG	2 cut(s)	399, 1300
BseJI	GATNNNNATC	1 cut(s)	540
BseLI	CCNNNNNNNGG	2 cut(s)	905, 973
BseMII	CTCAG	2 cut(s)	71, 385
BseNI	ACTGG	1 cut(s)	641
BseRI	GAGGAG	9 cut(s)	167, 214, 564, 567, 809, 1229, 1232, 1235, 1259
BseX3I	CGGCCG	1 cut(s)	352
BseXI	GCAGC	3 cut(s)	237, 265, 282
BseYI	CCCAGC	2 cut(s)	59, 97
BsgI	GTGCAG	1 cut(s)	440
Bsh1236I	CGCG	5 cut(s)	105, 212, 357, 425, 729
Bsh1285I	CGRYCG	1 cut(s)	355
BshFI	GGCC	9 cut(s)	32, 102, 164, 354, 825, 831, 966, 1050, 1102
BshNI	GGYRCC	2 cut(s)	712, 805
BsiEI	CGRYCG	1 cut(s)	355
BsiHKAI	GWGCWC	2 cut(s)	159, 558
BsiHKCI	CYCGRG	4 cut(s)	685, 860, 1004, 1096
BsiSI	CCGG	7 cut(s)	327, 351, 491, 698, 750, 904, 1127
BslFI	GGGAC	4 cut(s)	333, 802, 841, 998
BslI	CCNNNNNNNGG	2 cut(s)	905, 973
BsmAI	GTCTC	2 cut(s)	917, 1163
BsmBI	CGTCTC	2 cut(s)	917, 1163
BsmFI	GGGAC	4 cut(s)	333, 802, 841, 998
BsnI	GGCC	9 cut(s)	32, 102, 164, 354, 825, 831, 966, 1050, 1102
BsoBI	CYCGRG	4 cut(s)	685, 860, 1004, 1096
Bsp1286I	GDGCHC	2 cut(s)	159, 558
Bsp19I	CCATGG	2 cut(s)	27, 826
BspANI	GGCC	9 cut(s)	32, 102, 164, 354, 825, 831, 966, 1050, 1102
BspCNI	CTCAG	2 cut(s)	72, 384
BspFNI	CGCG	5 cut(s)	105, 212, 357, 425, 729
BspHI	TCATGA	1 cut(s)	501
BspLI	GGNNCC	7 cut(s)	57, 109, 714, 807, 857, 986, 1014
BspPI	GGATC	5 cut(s)	499, 612, 916, 1007, 1020
BspQI	GCTCTTC	1 cut(s)	209
BspT107I	GGYRCC	2 cut(s)	712, 805
BsrBI	CCGCTC	3 cut(s)	196, 283, 391
BsrFI	RCCGGY	2 cut(s)	326, 1126
BsrI	ACTGG	1 cut(s)	641
BssAI	RCCGGY	2 cut(s)	326, 1126
BssECI	CCNNGG	7 cut(s)	27, 103, 111, 180, 826, 859, 904
BssNI	GRCGYC	4 cut(s)	48, 713, 806, 843
BssT1I	CCWWGG	4 cut(s)	27, 111, 180, 826
Bst4CI	ACNGT	7 cut(s)	55, 347, 518, 709, 890, 900, 1174
Bst6I	CTCTTC	2 cut(s)	209, 232
BstACI	GRCGYC	4 cut(s)	48, 713, 806, 843
BstBAI	YACGTR	1 cut(s)	802
BstC8I	GCNNGC	4 cut(s)	324, 328, 662, 1128
BstDEI	CTNAG	3 cut(s)	80, 371, 959
BstDSI	CCRYGG	3 cut(s)	27, 103, 826
BstF5I	GGATG	2 cut(s)	399, 1300
BstFNI	CGCG	5 cut(s)	105, 212, 357, 425, 729
BstH2I	RGCGCY	4 cut(s)	79, 661, 716, 809
BstHHI	GCGC	5 cut(s)	78, 660, 715, 808, 1126
BstMAI	GTCTC	2 cut(s)	917, 1163
BstMCI	CGRYCG	1 cut(s)	355
BstMWI	GCNNNNNNNGC	6 cut(s)	225, 295, 332, 593, 652, 661
BstSCI	CCNGG	2 cut(s)	349, 903
BstUI	CGCG	5 cut(s)	105, 212, 357, 425, 729
BstV1I	GCAGC	3 cut(s)	237, 265, 282
BstX2I	RGATCY	2 cut(s)	908, 1012
BstXI	CCANNNNNNTGG	1 cut(s)	118
BstYI	RGATCY	2 cut(s)	908, 1012
BstZI	CGGCCG	1 cut(s)	352
BsuI	GTATCC	1 cut(s)	930
BsuRI	GGCC	9 cut(s)	32, 102, 164, 354, 825, 831, 966, 1050, 1102
BtgI	CCRYGG	3 cut(s)	27, 103, 826
BtsCI	GGATG	2 cut(s)	399, 1300
BtsIMutI	CAGTG	2 cut(s)	312, 648
Cac8I	GCNNGC	4 cut(s)	324, 328, 662, 1128
CaiI	CAGNNNCTG	1 cut(s)	79
CciI	TCATGA	1 cut(s)	501
CfoI	GCGC	5 cut(s)	78, 660, 715, 808, 1126
Cfr10I	RCCGGY	2 cut(s)	326, 1126
Cfr13I	GGNCC	5 cut(s)	100, 823, 855, 985, 1048
Cfr42I	CCGCGG	1 cut(s)	106
CseI	GACGC	4 cut(s)	37, 218, 718, 832
Csp6I	GTAC	6 cut(s)	232, 260, 799, 886, 891, 1108
CviAII	CATG	6 cut(s)	28, 160, 299, 502, 827, 833
CviQI	GTAC	6 cut(s)	232, 260, 799, 886, 891, 1108
DdeI	CTNAG	3 cut(s)	80, 371, 959
DinI	GGCGCC	2 cut(s)	714, 807
DraI	TTTAAA	1 cut(s)	1063
DrdI	GACNNNNNNGTC	2 cut(s)	47, 842
DseDI	GACNNNNNNGTC	2 cut(s)	47, 842
EaeI	YGGCCR	3 cut(s)	30, 352, 829
EagI	CGGCCG	1 cut(s)	352
Eam1104I	CTCTTC	2 cut(s)	209, 232
EarI	CTCTTC	2 cut(s)	209, 232
EciI	GGCGGA	2 cut(s)	443, 1145
Ecl136II	GAGCTC	2 cut(s)	157, 556
EclXI	CGGCCG	1 cut(s)	352
Eco130I	CCWWGG	4 cut(s)	27, 111, 180, 826
Eco24I	GRGCYC	2 cut(s)	159, 558
Eco47I	GGWCC	2 cut(s)	855, 985
Eco52I	CGGCCG	1 cut(s)	352
Eco53kI	GAGCTC	2 cut(s)	157, 556
Eco88I	CYCGRG	4 cut(s)	685, 860, 1004, 1096
EcoICRI	GAGCTC	2 cut(s)	157, 556
EcoO109I	RGGNCCY	1 cut(s)	985
EcoT14I	CCWWGG	4 cut(s)	27, 111, 180, 826
EcoT22I	ATGCAT	1 cut(s)	1363
EcoT38I	GRGCYC	2 cut(s)	159, 558
EgeI	GGCGCC	2 cut(s)	714, 807
EheI	GGCGCC	2 cut(s)	714, 807
ErhI	CCWWGG	4 cut(s)	27, 111, 180, 826
Esp3I	CGTCTC	2 cut(s)	917, 1163
FaeI	CATG	6 cut(s)	31, 163, 302, 505, 830, 836
FaiI	YATR	9 cut(s)	29, 161, 300, 503, 527, 614, 626, 828, 834
FaqI	GGGAC	4 cut(s)	333, 802, 841, 998
FatI	CATG	6 cut(s)	27, 159, 298, 501, 826, 832
FauI	CCCGC	1 cut(s)	288
FokI	GGATG	2 cut(s)	406, 1307
FriOI	GRGCYC	2 cut(s)	159, 558
GlaI	GCGC	5 cut(s)	77, 659, 714, 807, 1125
GsaI	CCCAGC	2 cut(s)	63, 101
HaeII	RGCGCY	4 cut(s)	79, 661, 716, 809
HaeIII	GGCC	9 cut(s)	32, 102, 164, 354, 825, 831, 966, 1050, 1102
HapII	CCGG	7 cut(s)	327, 351, 491, 698, 750, 904, 1127
HgaI	GACGC	4 cut(s)	37, 218, 718, 832
HhaI	GCGC	5 cut(s)	78, 660, 715, 808, 1126
Hin1I	GRCGYC	4 cut(s)	48, 713, 806, 843
Hin1II	CATG	6 cut(s)	31, 163, 302, 505, 830, 836
Hin6I	GCGC	5 cut(s)	76, 658, 713, 806, 1124
HinP1I	GCGC	5 cut(s)	76, 658, 713, 806, 1124
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HinfI	GANTC	5 cut(s)	240, 571, 1026, 1079, 1092
HpaII	CCGG	7 cut(s)	327, 351, 491, 698, 750, 904, 1127
HphI	GGTGA	3 cut(s)	142, 1189, 1208
Hpy166II	GTNNAC	2 cut(s)	475, 733
Hpy188III	TCNNGA	5 cut(s)	308, 502, 1006, 1096, 1115
Hpy8I	GTNNAC	2 cut(s)	475, 733
Hpy99I	CGWCG	7 cut(s)	47, 143, 224, 270, 536, 848, 976
HpyAV	CCTTC	4 cut(s)	127, 1003, 1274, 1313
HpyCH4III	ACNGT	7 cut(s)	55, 347, 518, 709, 890, 900, 1174
HpyCH4IV	ACGT	3 cut(s)	265, 801, 926
HpyCH4V	TGCA	5 cut(s)	298, 457, 646, 681, 1361
HpyF10VI	GCNNNNNNNGC	6 cut(s)	225, 295, 332, 593, 652, 661
HpyF3I	CTNAG	3 cut(s)	80, 371, 959
HpySE526I	ACGT	3 cut(s)	265, 801, 926
Hsp92I	GRCGYC	4 cut(s)	48, 713, 806, 843
Hsp92II	CATG	6 cut(s)	31, 163, 302, 505, 830, 836
HspAI	GCGC	5 cut(s)	76, 658, 713, 806, 1124
KasI	GGCGCC	2 cut(s)	712, 805
KroI	GCCGGC	2 cut(s)	326, 1126
KroNI	GCCGGC	2 cut(s)	328, 1128
KspI	CCGCGG	1 cut(s)	106
LguI	GCTCTTC	1 cut(s)	209
LmnI	GCTCC	2 cut(s)	107, 154
Lsp1109I	GCAGC	3 cut(s)	237, 265, 282
LweI	GCATC	1 cut(s)	1348
MaeII	ACGT	3 cut(s)	265, 801, 926
MaeIII	GTNAC	3 cut(s)	49, 518, 1135
MbiI	CCGCTC	3 cut(s)	196, 283, 391
MboII	GAAGA	3 cut(s)	226, 249, 1211
MfeI	CAATTG	1 cut(s)	364
MflI	RGATCY	2 cut(s)	908, 1012
MhlI	GDGCHC	2 cut(s)	159, 558
MlsI	TGGCCA	1 cut(s)	831
MluNI	TGGCCA	1 cut(s)	831
Mly113I	GGCGCC	2 cut(s)	713, 806
MlyI	GAGTC	2 cut(s)	249, 565
Mox20I	TGGCCA	1 cut(s)	831
Mph1103I	ATGCAT	1 cut(s)	1363
MreI	CGCCGGCG	1 cut(s)	1126
MroNI	GCCGGC	2 cut(s)	326, 1126
MroXI	GAANNNNTTC	1 cut(s)	435
MscI	TGGCCA	1 cut(s)	831
MseI	TTAA	3 cut(s)	167, 980, 1062
MslI	CAYNNNNRTG	1 cut(s)	831
Msp20I	TGGCCA	1 cut(s)	831
MspA1I	CMGCKG	1 cut(s)	105
MspI	CCGG	7 cut(s)	327, 351, 491, 698, 750, 904, 1127
MspR9I	CCNGG	2 cut(s)	351, 905
MunI	CAATTG	1 cut(s)	364
MvnI	CGCG	5 cut(s)	105, 212, 357, 425, 729
MwoI	GCNNNNNNNGC	6 cut(s)	225, 295, 332, 593, 652, 661
NaeI	GCCGGC	2 cut(s)	328, 1128
NarI	GGCGCC	2 cut(s)	713, 806
NciI	CCSGG	2 cut(s)	351, 905
NcoI	CCATGG	2 cut(s)	27, 826
NgoMIV	GCCGGC	2 cut(s)	326, 1126
NlaIII	CATG	6 cut(s)	31, 163, 302, 505, 830, 836
NlaIV	GGNNCC	7 cut(s)	57, 109, 714, 807, 857, 986, 1014
NmuCI	GTSAC	2 cut(s)	49, 518
NsiI	ATGCAT	1 cut(s)	1363
PaeR7I	CTCGAG	2 cut(s)	1004, 1096
PagI	TCATGA	1 cut(s)	501
PciSI	GCTCTTC	1 cut(s)	209
PcsI	WCGNNNNNNNCGW	4 cut(s)	216, 537, 807, 1103
PdiI	GCCGGC	2 cut(s)	328, 1128
PdmI	GAANNNNTTC	1 cut(s)	435
PfeI	GAWTC	3 cut(s)	1026, 1079, 1092
PflFI	GACNNNGTC	1 cut(s)	47
PleI	GAGTC	2 cut(s)	248, 565
PluTI	GGCGCC	2 cut(s)	716, 809
PpsI	GAGTC	2 cut(s)	248, 565
Ppu21I	YACGTR	1 cut(s)	802
PpuMI	RGGWCCY	1 cut(s)	985
Psp124BI	GAGCTC	2 cut(s)	159, 558
Psp5II	RGGWCCY	1 cut(s)	985
PspFI	CCCAGC	2 cut(s)	59, 97
PspN4I	GGNNCC	7 cut(s)	57, 109, 714, 807, 857, 986, 1014
PspPI	GGNCC	5 cut(s)	100, 823, 855, 985, 1048
PspPPI	RGGWCCY	1 cut(s)	985
PstNI	CAGNNNCTG	1 cut(s)	79
PsuI	RGATCY	2 cut(s)	908, 1012
PsyI	GACNNNGTC	1 cut(s)	47
RsaI	GTAC	6 cut(s)	233, 261, 800, 887, 892, 1109
RsaNI	GTAC	6 cut(s)	232, 260, 799, 886, 891, 1108
RseI	CAYNNNNRTG	1 cut(s)	831
SacI	GAGCTC	2 cut(s)	159, 558
SacII	CCGCGG	1 cut(s)	106
SapI	GCTCTTC	1 cut(s)	209
SaqAI	TTAA	3 cut(s)	167, 980, 1062
Sau96I	GGNCC	5 cut(s)	100, 823, 855, 985, 1048
ScaI	AGTACT	1 cut(s)	261
SchI	GAGTC	2 cut(s)	249, 565
ScrFI	CCNGG	2 cut(s)	351, 905
SduI	GDGCHC	2 cut(s)	159, 558
SfaNI	GCATC	1 cut(s)	1348
SfoI	GGCGCC	2 cut(s)	714, 807
Sfr274I	CTCGAG	2 cut(s)	1004, 1096
Sfr303I	CCGCGG	1 cut(s)	106
SgrAI	CRCCGGYG	1 cut(s)	1126
SgrBI	CCGCGG	1 cut(s)	106
SinI	GGWCC	2 cut(s)	855, 985
SlaI	CTCGAG	2 cut(s)	1004, 1096
SmiMI	CAYNNNNRTG	1 cut(s)	831
SmlI	CTYRAG	2 cut(s)	1004, 1096
SmoI	CTYRAG	2 cut(s)	1004, 1096
SspDI	GGCGCC	2 cut(s)	712, 805
SspI	AATATT	1 cut(s)	1188
SstI	GAGCTC	2 cut(s)	159, 558
StyD4I	CCNGG	2 cut(s)	349, 903
StyI	CCWWGG	4 cut(s)	27, 111, 180, 826
TaaI	ACNGT	7 cut(s)	55, 347, 518, 709, 890, 900, 1174
TaiI	ACGT	3 cut(s)	268, 804, 929
TaqII	GACCGA	1 cut(s)	401
TatI	WGTACW	1 cut(s)	259
TauI	GCSGC	7 cut(s)	105, 149, 357, 428, 658, 718, 1105
TfiI	GAWTC	3 cut(s)	1026, 1079, 1092
Tru1I	TTAA	3 cut(s)	167, 980, 1062
Tru9I	TTAA	3 cut(s)	167, 980, 1062
TscAI	CASTG	2 cut(s)	319, 648
TseFI	GTSAC	2 cut(s)	49, 518
TseI	GCWGC	3 cut(s)	225, 253, 295
Tsp45I	GTSAC	2 cut(s)	49, 518
TspGWI	ACGGA	4 cut(s)	474, 806, 1154, 1158
TspRI	CASTG	2 cut(s)	319, 648
Tth111I	GACNNNGTC	1 cut(s)	47
VpaK11BI	GGWCC	2 cut(s)	855, 985
XapI	RAATTY	3 cut(s)	619, 997, 1327
XhoI	CTCGAG	2 cut(s)	1004, 1096
XmnI	GAANNNNTTC	1 cut(s)	435
ZrmI	AGTACT	1 cut(s)	261
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.