AT3G25640

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013999
Physical Location & Seq
Forward (+)
9333662 .. 9334889
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25640.2

Sequence Viewer

Length: 804 bp
ATGAAATCAATCTTAGCCAACACTTCTCTCGACTCTTCATTCTCTCTTTCTAAACGTTACTTCAATTGGAAGAAGAAGAAAGTTCAAGAAGACGACGACGAAGAAGAGGAAGAAGAAGAAGACGATTACAACCACAACGAGAAAAAAGTCTTGACCCGTTTCAATTTCTCCTCTGACCCGACCCGACCGGATCAGTTCAATGCCCAACAGATAATGAAGAAAAAAAAGAAAAAGACTCTCGAAAAGATCCGTTACGCTCTCGGGTTCTCTAACTCGGGTCTGGGTTTCCGGGTCGTGGGTACGTTATTCGGTAACCGTCGTGGACACGTGTACTTCGCCGTACAAGATGACCCGACTCGTTTACCTGCGGTGTTGATCCAGTTACCGACTCCAACGAGCGTTCTTGTCCGTGAAATGGCTTCAGGGCTAGTGAGAATCGCGCTCGAAACGGCTGCGTATAAGACAGATTCAAAGAAGAAGCTTTTAGAAGAGTCCACGTGGAGAACGTATTGTAACGGTAAAAAATGTGGTTACGCGGCGAGGAAAGAGTGTGGAGAAGCGGAGTGGAAGGTGTTGAAGGCGGTTGGGCCTATAACAATGGGTGCTGGAGTTTTACCGGCGACTACGACGACGGTGGATGAAGAAGGGAATGGAGCGGTTGGGTCCGAAAAAGGTGAACTCATGTATATGAGAGCCCGGTTCGAGCGGGTTGTCGGGTCGAGAGACTCGGAGGCGTTTTACATGATGAACCCTGATGTGTCAAGTGGTGGACCTGAGCTTAGTGTGTATTTTCTAAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

267

Amino Acids

30.09

Weight (kDa)

8.76

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 124 - 292 1.9e-66 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25640 AT3G25640
fragaria_vesca FvH4_4g28270
malus_domestica MD13G1048700.v1.1 MD16G1050000.v1.1
prunus_persica Prupe.1G303400_v2.0.a1
pyrus_communis pycom16g04380
rosa_chinensis RchiOBHm_Chr4g0436141
rosa_laevigata RLG00000006537
rosa_multiflora Rmu_sc0022632.1_g000001
rosa_roxburghii Rroxscaffold_5G00377240
rosa_rugosa Rorug04G0292200
rosa_samantha Rh4AG346200 Rh4BG355300 Rh4CG369900 Rh4DG348900
rosa_wichuraiana Rw4G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 373
AccBSI CCGCTC 2 cut(s) 656, 706
AccII CGCG 2 cut(s) 440, 536
AciI CCGC 6 cut(s) 368, 536, 560, 581, 656, 706
AclI AACGTT 1 cut(s) 55
AclWI GGATC 3 cut(s) 198, 241, 370
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 2 cut(s) 328, 498
AfaI GTAC 3 cut(s) 301, 332, 342
AfiI CCNNNNNNNGG 2 cut(s) 295, 415
AflIII ACRYGT 2 cut(s) 325, 327
AgsI TTSAA 6 cut(s) 64, 86, 163, 199, 471, 577
AluBI AGCT 2 cut(s) 481, 778
AluI AGCT 2 cut(s) 481, 778
Alw26I GTCTC 1 cut(s) 717
AlwI GGATC 3 cut(s) 198, 241, 370
Ama87I CYCGRG 2 cut(s) 260, 274
AoxI GGCC 1 cut(s) 587
ApeKI GCWGC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 3 cut(s) 587, 663, 770
AsuC2I CCSGG 2 cut(s) 290, 697
AsuHPI GGTGA 1 cut(s) 686
AvaI CYCGRG 2 cut(s) 260, 274
AvaII GGWCC 2 cut(s) 663, 770
BanII GRGCYC 1 cut(s) 697
BbrPI CACGTG 2 cut(s) 328, 498
BbsI GAAGAC 2 cut(s) 96, 126
BbvI GCAGC 1 cut(s) 439
BceAI ACGGC 2 cut(s) 323, 465
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BcnI CCSGG 2 cut(s) 290, 697
BcoDI GTCTC 1 cut(s) 717
BfaI CTAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 373
BisI GCNGC 2 cut(s) 453, 537
BlsI GCNGC 2 cut(s) 454, 538
Bme1390I CCNGG 2 cut(s) 290, 697
Bme18I GGWCC 2 cut(s) 663, 770
BmeT110I CYCGRG 2 cut(s) 260, 274
BmgT120I GGNCC 3 cut(s) 587, 663, 770
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 2 cut(s) 290, 697
BpiI GAAGAC 2 cut(s) 96, 126
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 774
BpuMI CCSGG 2 cut(s) 290, 697
BsaAI YACGTR 2 cut(s) 328, 498
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 415
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 643
BseLI CCNNNNNNNGG 2 cut(s) 295, 415
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 1 cut(s) 160
BseXI GCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 440, 536
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 1 cut(s) 589
BsiEI CGRYCG 1 cut(s) 188
BsiHKCI CYCGRG 2 cut(s) 260, 274
BsiSI CCGG 4 cut(s) 188, 289, 617, 697
BslI CCNNNNNNNGG 2 cut(s) 295, 415
BsmAI GTCTC 1 cut(s) 717
BsnI GGCC 1 cut(s) 589
BsoBI CYCGRG 2 cut(s) 260, 274
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 3 cut(s) 190, 246, 375
BspACI CCGC 6 cut(s) 368, 536, 560, 581, 656, 706
BspANI GGCC 1 cut(s) 589
BspCNI CTCAG 1 cut(s) 766
BspFNI CGCG 2 cut(s) 440, 536
BspLI GGNNCC 1 cut(s) 664
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 3 cut(s) 198, 241, 370
BsrBI CCGCTC 2 cut(s) 656, 706
BsrFI RCCGGY 1 cut(s) 616
BsrI ACTGG 1 cut(s) 379
BssAI RCCGGY 1 cut(s) 616
BssMI GATC 3 cut(s) 190, 246, 375
Bst4CI ACNGT 3 cut(s) 317, 518, 634
Bst6I CTCTTC 3 cut(s) 40, 99, 483
BstBAI YACGTR 2 cut(s) 328, 498
BstDEI CTNAG 4 cut(s) 13, 774, 779, 794
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 2 cut(s) 440, 536
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 3 cut(s) 193, 249, 378
BstMAI GTCTC 1 cut(s) 717
BstMBI GATC 3 cut(s) 190, 246, 375
BstMCI CGRYCG 1 cut(s) 188
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 288, 695
BstUI CGCG 2 cut(s) 440, 536
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 2 cut(s) 96, 126
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 1 cut(s) 589
BtsCI GGATG 1 cut(s) 643
BveI ACCTGC 1 cut(s) 373
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 3 cut(s) 587, 663, 770
Csp6I GTAC 3 cut(s) 300, 331, 341
CviAII CATG 2 cut(s) 682, 742
CviJI RGCY 8 cut(s) 17, 419, 427, 452, 481, 589, 695, 778
CviKI_1 RGCY 8 cut(s) 17, 419, 427, 452, 481, 589, 695, 778
CviQI GTAC 3 cut(s) 300, 331, 341
DdeI CTNAG 4 cut(s) 13, 774, 779, 794
DpnI GATC 3 cut(s) 192, 248, 377
DpnII GATC 3 cut(s) 190, 246, 375
Eam1104I CTCTTC 3 cut(s) 40, 99, 483
EarI CTCTTC 3 cut(s) 40, 99, 483
Eco24I GRGCYC 1 cut(s) 697
Eco47I GGWCC 2 cut(s) 663, 770
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 2 cut(s) 328, 498
Eco88I CYCGRG 2 cut(s) 260, 274
Eco91I GGTNACC 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 697
FaeI CATG 2 cut(s) 685, 745
FaiI YATR 6 cut(s) 459, 593, 683, 687, 689, 743
FatI CATG 2 cut(s) 681, 741
FauI CCCGC 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 453, 537
FokI GGATG 1 cut(s) 650
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 2 cut(s) 453, 537
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 441
GluI GCNGC 2 cut(s) 453, 537
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 1 cut(s) 589
HapII CCGG 4 cut(s) 188, 289, 617, 697
HhaI GCGC 1 cut(s) 442
Hin1II CATG 2 cut(s) 685, 745
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 8 cut(s) 32, 235, 355, 388, 435, 467, 491, 725
HpaII CCGG 4 cut(s) 188, 289, 617, 697
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 6 cut(s) 323, 331, 362, 495, 677, 770
Hpy188I TCNGA 3 cut(s) 175, 667, 730
Hpy188III TCNNGA 5 cut(s) 29, 86, 151, 239, 720
Hpy8I GTNNAC 6 cut(s) 323, 331, 362, 495, 677, 770
Hpy99I CGWCG 5 cut(s) 98, 101, 321, 631, 634
HpyAV CCTTC 3 cut(s) 562, 571, 638
HpyCH4III ACNGT 3 cut(s) 317, 518, 634
HpyCH4IV ACGT 5 cut(s) 55, 302, 327, 497, 506
HpyF3I CTNAG 4 cut(s) 13, 774, 779, 794
HpySE526I ACGT 5 cut(s) 55, 302, 327, 497, 506
Hsp92II CATG 2 cut(s) 685, 745
HspAI GCGC 1 cut(s) 440
Kzo9I GATC 3 cut(s) 190, 246, 375
LmnI GCTCC 1 cut(s) 653
Lsp1109I GCAGC 1 cut(s) 439
MaeI CTAG 1 cut(s) 428
MaeII ACGT 5 cut(s) 55, 302, 327, 497, 506
MaeIII GTNAC 6 cut(s) 56, 251, 311, 381, 512, 530
MalI GATC 3 cut(s) 192, 248, 377
MbiI CCGCTC 2 cut(s) 656, 706
MboI GATC 3 cut(s) 190, 246, 375
MfeI CAATTG 1 cut(s) 64
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 2 cut(s) 64, 163
MlyI GAGTC 6 cut(s) 26, 229, 349, 382, 500, 719
MmeI TCCRAC 1 cut(s) 416
MnlI CCTC 4 cut(s) 100, 181, 534, 724
MseI TTAA 1 cut(s) 802
MslI CAYNNNNRTG 1 cut(s) 686
MspI CCGG 4 cut(s) 188, 289, 617, 697
MspR9I CCNGG 2 cut(s) 290, 697
MunI CAATTG 1 cut(s) 64
MvnI CGCG 2 cut(s) 440, 536
NciI CCSGG 2 cut(s) 290, 697
NdeII GATC 3 cut(s) 190, 246, 375
NlaIII CATG 2 cut(s) 685, 745
NlaIV GGNNCC 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PfeI GAWTC 2 cut(s) 435, 467
PkrI GCNGC 2 cut(s) 454, 538
PleI GAGTC 6 cut(s) 26, 229, 349, 382, 499, 719
PmaCI CACGTG 2 cut(s) 328, 498
PmlI CACGTG 2 cut(s) 328, 498
PpsI GAGTC 6 cut(s) 26, 229, 349, 382, 499, 719
Ppu21I YACGTR 2 cut(s) 328, 498
Psp1406I AACGTT 1 cut(s) 55
PspCI CACGTG 2 cut(s) 328, 498
PspEI GGTNACC 1 cut(s) 311
PspN4I GGNNCC 1 cut(s) 664
PspPI GGNCC 3 cut(s) 587, 663, 770
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 3 cut(s) 301, 332, 342
RsaNI GTAC 3 cut(s) 300, 331, 341
RseI CAYNNNNRTG 1 cut(s) 686
SaqAI TTAA 1 cut(s) 802
SatI GCNGC 2 cut(s) 453, 537
Sau3AI GATC 3 cut(s) 190, 246, 375
Sau96I GGNCC 3 cut(s) 587, 663, 770
SchI GAGTC 6 cut(s) 26, 229, 349, 382, 500, 719
ScrFI CCNGG 2 cut(s) 290, 697
SduI GDGCHC 1 cut(s) 697
SinI GGWCC 2 cut(s) 663, 770
SmiMI CAYNNNNRTG 1 cut(s) 686
Sse9I AATT 2 cut(s) 64, 163
SsiI CCGC 6 cut(s) 368, 536, 560, 581, 656, 706
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 288, 695
TaaI ACNGT 3 cut(s) 317, 518, 634
TaiI ACGT 5 cut(s) 58, 305, 330, 500, 509
TaqI TCGA 5 cut(s) 30, 240, 444, 702, 719
TasI AATT 2 cut(s) 64, 163
TatI WGTACW 1 cut(s) 330
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 435, 467
Tru1I TTAA 1 cut(s) 802
Tru9I TTAA 1 cut(s) 802
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 17, 27, 230, 654, 761
TspGWI ACGGA 2 cut(s) 239, 398
VpaK11BI GGWCC 2 cut(s) 663, 770
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.