MD13G1048700.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013999
Physical Location & Seq
Reverse (-)
3421693 .. 3422490
798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1048700.v1.1.491

Sequence Viewer

Length: 798 bp
ATGAGGACAATCATGGCAGCAAAGACCCTTCACGACTCATCACTCTCTTTCTCCAGGAGGTACTTCCACTGGAAAAAGAAATCTGAGGACGAGGAGGACGACGACCAGGAAATCTTAAACTTCAGCCACTTGCATTTCTCGGAAGAATCATCGGAGAAAGAAAATCACGAGGAGCTCAGAATCCCAGTCGCCCCGGCAGAAACTGCAGTAATCCCACCAAAGAAACACTCTTTAATGTCGGTCTCCAAGCTCCGCTCGGCTCTCACGGTGTTTGGAAAGAGCCACCGCTCCCACCGCCCCGTACTCGGCCGCAGAGTGATGGGCACTCTGTTCGGCTACCGCCGTGGACACGTCCACCTGGCATTTCAAGACGAGCCCAAGTCGGCGCCCGCCTTCCTGATCGAGCTGGCGACGCCGACGAGCGTTCTGGTGCGAGAAATGGCATCTGGGTTGGTGCGGATAGCGCTGGAGACGGAGAAGAAAGCGGAGAAGAAGGGTTTTAAGTTACTGGAGGAGCCGCTGTGGAGGACTTACTGCAACGGGAAGAAATGCGGGTTTGCTATGAAGCGGGAGTGCGGCGGTGAGGAGTGGAAGGTGTTGAAAGCTCTGGAGGCGATTTCAATGGGGGCGGGAGTTCTGCCGGCGAGTGACGACGGCGTTGGGGCGGAGGGGGAGCTCATGTACATGAGAGCTAAGTTTGAGAGGGTTGCGGGGTCCAAGGACTCTGAGGCATTTTACATGATGAACCCTGATGGCTCTGGGGGTCCTGAGCTTAGCATTTATTTGCTTAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

266

Amino Acids

29.76

Weight (kDa)

8.32

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 106 - 264 4.3e-72 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25640 AT3G25640
fragaria_vesca FvH4_4g28270
malus_domestica MD13G1048700.v1.1 MD16G1050000.v1.1
prunus_persica Prupe.1G303400_v2.0.a1
pyrus_communis pycom16g04380
rosa_chinensis RchiOBHm_Chr4g0436141
rosa_laevigata RLG00000006537
rosa_multiflora Rmu_sc0022632.1_g000001
rosa_roxburghii Rroxscaffold_5G00377240
rosa_rugosa Rorug04G0292200
rosa_samantha Rh4AG346200 Rh4BG355300 Rh4CG369900 Rh4DG348900
rosa_wichuraiana Rw4G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 385
AccBSI CCGCTC 2 cut(s) 255, 288
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 106
AcyI GRCGYC 2 cut(s) 386, 413
AfaI GTAC 3 cut(s) 62, 303, 683
AfeI AGCGCT 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 3 cut(s) 368, 601, 621
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 3 cut(s) 53, 105, 357
AluBI AGCT 7 cut(s) 175, 250, 406, 605, 676, 692, 772
AluI AGCT 7 cut(s) 175, 250, 406, 605, 676, 692, 772
Alw21I GWGCWC 2 cut(s) 177, 678
Alw26I GTCTC 2 cut(s) 247, 464
AlwNI CAGNNNCTG 1 cut(s) 203
Aor51HI AGCGCT 1 cut(s) 465
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 388, 466
AspS9I GGNCC 2 cut(s) 714, 764
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 593
AvaII GGWCC 2 cut(s) 714, 764
BaeGI GKGCMC 1 cut(s) 326
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 3 cut(s) 177, 378, 678
BauI CACGAG 1 cut(s) 167
Bbv12I GWGCWC 2 cut(s) 177, 678
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 313, 746
BceAI ACGGC 2 cut(s) 327, 670
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 3 cut(s) 55, 107, 359
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 247, 464
BfmI CTRYAG 1 cut(s) 204
BfoI RGCGCY 2 cut(s) 389, 467
BisI GCNGC 4 cut(s) 18, 310, 518, 577
BlpI GCTNAGC 1 cut(s) 773
BlsI GCNGC 4 cut(s) 19, 311, 519, 578
Bme1390I CCNGG 4 cut(s) 55, 107, 194, 359
Bme18I GGWCC 2 cut(s) 714, 764
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 2 cut(s) 714, 764
BmiI GGNNCC 4 cut(s) 387, 516, 715, 765
BmrFI CCNGG 4 cut(s) 55, 107, 194, 359
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 179
BpmI CTGGAG 4 cut(s) 37, 488, 530, 629
Bpu10I CCTNAGC 1 cut(s) 768
Bpu1102I GCTNAGC 1 cut(s) 773
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 2 cut(s) 386, 413
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 3 cut(s) 192, 343, 717
BsaXI ACNNNNNCTCC 4 cut(s) 506, 536, 665, 695
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 3 cut(s) 74, 185, 513
BseBI CCWGG 3 cut(s) 55, 107, 359
BseDI CCNNGG 3 cut(s) 192, 343, 717
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 4 cut(s) 75, 190, 717, 759
BseNI ACTGG 3 cut(s) 74, 185, 513
BseRI GAGGAG 4 cut(s) 107, 185, 527, 599
BseSI GKGCMC 1 cut(s) 326
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 385
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 2 cut(s) 177, 678
BsiSI CCGG 2 cut(s) 194, 641
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 247, 464
BsmBI CGTCTC 1 cut(s) 464
BsnI GGCC 1 cut(s) 309
Bso31I GGTCTC 1 cut(s) 247
Bsp1286I GDGCHC 4 cut(s) 177, 326, 378, 678
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 1 cut(s) 399
Bsp1720I GCTNAGC 1 cut(s) 773
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 4 cut(s) 76, 189, 718, 760
BspLI GGNNCC 4 cut(s) 387, 516, 715, 765
BspMAI CTGCAG 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 385
BspTNI GGTCTC 1 cut(s) 247
BsrBI CCGCTC 2 cut(s) 255, 288
BsrFI RCCGGY 1 cut(s) 640
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 3 cut(s) 74, 185, 513
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 3 cut(s) 192, 343, 717
BssMI GATC 1 cut(s) 399
BssNI GRCGYC 2 cut(s) 386, 413
BssSI CACGAG 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 55, 107, 359
Bst4CI ACNGT 1 cut(s) 268
BstACI GRCGYC 2 cut(s) 386, 413
BstAPI GCANNNNNTGC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 3 cut(s) 390, 408, 642
BstDEI CTNAG 7 cut(s) 84, 176, 693, 726, 768, 773, 788
BstDSI CCRYGG 1 cut(s) 343
BstH2I RGCGCY 2 cut(s) 389, 467
BstHHI GCGC 2 cut(s) 388, 466
BstKTI GATC 1 cut(s) 402
BstMAI GTCTC 2 cut(s) 247, 464
BstMBI GATC 1 cut(s) 399
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 5 cut(s) 203, 294, 412, 463, 611
BstNI CCWGG 3 cut(s) 55, 107, 359
BstSCI CCNGG 4 cut(s) 53, 105, 192, 357
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 29
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 343
BtrI CACGTC 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 390, 408, 642
CaiI CAGNNNCTG 1 cut(s) 203
CfoI GCGC 2 cut(s) 388, 466
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 714, 764
CseI GACGC 1 cut(s) 421
Csp6I GTAC 3 cut(s) 61, 302, 682
CviAII CATG 4 cut(s) 13, 679, 685, 739
CviQI GTAC 3 cut(s) 61, 302, 682
DdeI CTNAG 7 cut(s) 84, 176, 693, 726, 768, 773, 788
DinI GGCGCC 1 cut(s) 387
DpnI GATC 1 cut(s) 401
DpnII GATC 1 cut(s) 399
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 2 cut(s) 175, 676
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 3 cut(s) 177, 378, 678
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 2 cut(s) 714, 764
Eco47III AGCGCT 1 cut(s) 465
Eco52I CGGCCG 1 cut(s) 307
Eco53kI GAGCTC 2 cut(s) 175, 676
Eco57I CTGAAG 1 cut(s) 106
EcoICRI GAGCTC 2 cut(s) 175, 676
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 3 cut(s) 53, 105, 357
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 3 cut(s) 177, 378, 678
EgeI GGCGCC 1 cut(s) 387
EheI GGCGCC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 717
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 4 cut(s) 16, 682, 688, 742
FaiI YATR 5 cut(s) 14, 563, 680, 686, 740
FatI CATG 4 cut(s) 12, 678, 684, 738
FauI CCCGC 5 cut(s) 397, 545, 561, 622, 703
Fnu4HI GCNGC 4 cut(s) 18, 310, 518, 577
FriOI GRGCYC 3 cut(s) 177, 378, 678
Fsp4HI GCNGC 4 cut(s) 18, 310, 518, 577
GlaI GCGC 2 cut(s) 387, 465
GluI GCNGC 4 cut(s) 18, 310, 518, 577
GsuI CTGGAG 4 cut(s) 37, 488, 530, 629
HaeII RGCGCY 2 cut(s) 389, 467
HaeIII GGCC 1 cut(s) 309
HapII CCGG 2 cut(s) 194, 641
HgaI GACGC 1 cut(s) 421
HhaI GCGC 2 cut(s) 388, 466
Hin1I GRCGYC 2 cut(s) 386, 413
Hin1II CATG 4 cut(s) 16, 682, 688, 742
Hin6I GCGC 2 cut(s) 386, 464
HinP1I GCGC 2 cut(s) 386, 464
HinfI GANTC 4 cut(s) 35, 146, 180, 722
HpaII CCGG 2 cut(s) 194, 641
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 2 cut(s) 347, 355
Hpy188I TCNGA 5 cut(s) 85, 142, 154, 179, 727
Hpy188III TCNNGA 6 cut(s) 32, 167, 368, 397, 608, 767
Hpy8I GTNNAC 2 cut(s) 347, 355
Hpy99I CGWCG 4 cut(s) 104, 415, 421, 656
HpyAV CCTTC 4 cut(s) 38, 403, 487, 586
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 3 cut(s) 133, 206, 537
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 294, 412, 463, 611
HpyF3I CTNAG 7 cut(s) 84, 176, 693, 726, 768, 773, 788
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 2 cut(s) 386, 413
Hsp92II CATG 4 cut(s) 16, 682, 688, 742
HspAI GCGC 2 cut(s) 386, 464
KasI GGCGCC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Kzo9I GATC 1 cut(s) 399
LmnI GCTCC 5 cut(s) 172, 255, 293, 514, 673
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 452
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 2 cut(s) 504, 647
MalI GATC 1 cut(s) 401
MbiI CCGCTC 2 cut(s) 255, 288
MboI GATC 1 cut(s) 399
MboII GAAGA 4 cut(s) 155, 490, 502, 556
MhlI GDGCHC 4 cut(s) 177, 326, 378, 678
Mly113I GGCGCC 1 cut(s) 386
MlyI GAGTC 2 cut(s) 29, 716
MroNI GCCGGC 1 cut(s) 640
MseI TTAA 3 cut(s) 116, 233, 501
MslI CAYNNNNRTG 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 2 cut(s) 194, 641
MspR9I CCNGG 4 cut(s) 55, 107, 194, 359
MvaI CCWGG 3 cut(s) 55, 107, 359
MwoI GCNNNNNNNGC 5 cut(s) 203, 294, 412, 463, 611
NaeI GCCGGC 1 cut(s) 642
NarI GGCGCC 1 cut(s) 386
NciI CCSGG 1 cut(s) 194
NdeII GATC 1 cut(s) 399
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 4 cut(s) 16, 682, 688, 742
NlaIV GGNNCC 4 cut(s) 387, 516, 715, 765
NmeAIII GCCGAG 2 cut(s) 236, 285
NmuCI GTSAC 1 cut(s) 647
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 642
PfeI GAWTC 2 cut(s) 146, 180
PfoI TCCNGGA 1 cut(s) 53
PkrI GCNGC 4 cut(s) 19, 311, 519, 578
PleI GAGTC 2 cut(s) 29, 716
PluTI GGCGCC 1 cut(s) 389
PpsI GAGTC 2 cut(s) 29, 716
PpuMI RGGWCCY 1 cut(s) 764
Psp124BI GAGCTC 2 cut(s) 177, 678
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 3 cut(s) 53, 105, 357
PspGI CCWGG 3 cut(s) 53, 105, 357
PspN4I GGNNCC 4 cut(s) 387, 516, 715, 765
PspPI GGNCC 2 cut(s) 714, 764
PspPPI RGGWCCY 1 cut(s) 764
PstI CTGCAG 1 cut(s) 208
PstNI CAGNNNCTG 1 cut(s) 203
RsaI GTAC 3 cut(s) 62, 303, 683
RsaNI GTAC 3 cut(s) 61, 302, 682
RseI CAYNNNNRTG 1 cut(s) 683
SacI GAGCTC 2 cut(s) 177, 678
SaqAI TTAA 3 cut(s) 116, 233, 501
SatI GCNGC 4 cut(s) 18, 310, 518, 577
Sau3AI GATC 1 cut(s) 399
Sau96I GGNCC 2 cut(s) 714, 764
SchI GAGTC 2 cut(s) 29, 716
ScrFI CCNGG 4 cut(s) 55, 107, 194, 359
SduI GDGCHC 4 cut(s) 177, 326, 378, 678
SfaNI GCATC 1 cut(s) 452
SfcI CTRYAG 1 cut(s) 204
SfoI GGCGCC 1 cut(s) 387
SinI GGWCC 2 cut(s) 714, 764
SmiMI CAYNNNNRTG 1 cut(s) 683
SspDI GGCGCC 1 cut(s) 385
SstI GAGCTC 2 cut(s) 177, 678
StyD4I CCNGG 4 cut(s) 53, 105, 192, 357
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 354
TaqI TCGA 1 cut(s) 402
TaqII GACCGA 1 cut(s) 229
TatI WGTACW 1 cut(s) 681
TauI GCSGC 3 cut(s) 312, 520, 579
TfiI GAWTC 2 cut(s) 146, 180
Tru1I TTAA 3 cut(s) 116, 233, 501
Tru9I TTAA 3 cut(s) 116, 233, 501
TscAI CASTG 1 cut(s) 74
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 2 cut(s) 578, 758
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 1 cut(s) 74
VpaK11BI GGWCC 2 cut(s) 714, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.