MD16G1050000.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013999
Physical Location & Seq
Reverse (-)
3527351 .. 3528148
798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1050000.v1.1.491

Sequence Viewer

Length: 798 bp
ATGAGGACAATCATGGCAGCAAAGACCCCTCACGACTCATCTTTCTCTTTCTCCAGGAGGTACTTCCACTGGAAAAAGAAATCCGAGGACGAGGTGGACGACGACCAGGGAATACTAAACTTAAGCTACTCACATTTCTCCGAGAAATCATCGGAGAAAGATGATCACGACGAGCTCAGAATCCCAGTCGTCCATGCAGAAGTTGCAGTAATCCCACCAAAGAAACACTCTTTAATGTCAGTCTCTAAGCTCCGCTCAGCTCTCACGGTGTTTGGGAAAAGCCACCGCTCTCAGCGCCCCGTCCTCGGCCGCAGAGTGATGGGCACTCTGTTCGGCTACCGCCGCGGACATGTTCATTTGGCATTTCAAGACGAACCCAAGTCGCCCCCTGCCTTCCTGATCGAGCTGGCGACGCCGACGAGCGTTCTGGTGCGAGAAATGGCATCTGGGTTGGTGCGGGTCGCGCTGGAGACGGAGAAGAAAGCGGAGAATAAGGGTTTGAAGTTGCTGGAGGAGCCGCTGTGGAGGACTTACTGCAACGGGAAGAAATGCGGCTTTGCTATGAAGCGGGAGTGCGGCGGTGAGGAGTGGAAGGTGCTGAAAGCTCTGGAGGCGATTTCAATGGGGGCGGGAGTTCTGCCGGCGAGTGACGACGGCGTTGGGGCGGAGGGGGAGCTTATGTACATGAGAGCAAAGTTTGAGAGGGTTGCGGGGTCCAAGGACTCTGAGGCATTTTACATGATGAACCCTGATGGCTCTGGAGGTCCTGAGCTTAGCATTTATCTGCTTAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

266

Amino Acids

29.63

Weight (kDa)

8.82

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 106 - 264 9.8e-72 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25640 AT3G25640
fragaria_vesca FvH4_4g28270
malus_domestica MD13G1048700.v1.1 MD16G1050000.v1.1
prunus_persica Prupe.1G303400_v2.0.a1
pyrus_communis pycom16g04380
rosa_chinensis RchiOBHm_Chr4g0436141
rosa_laevigata RLG00000006537
rosa_multiflora Rmu_sc0022632.1_g000001
rosa_roxburghii Rroxscaffold_5G00377240
rosa_rugosa Rorug04G0292200
rosa_samantha Rh4AG346200 Rh4BG355300 Rh4CG369900 Rh4DG348900
rosa_wichuraiana Rw4G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 255, 288
AccII CGCG 2 cut(s) 345, 464
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 2 cut(s) 62, 683
AfiI CCNNNNNNNGG 1 cut(s) 305
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 3 cut(s) 368, 502, 621
AjnI CCWGG 2 cut(s) 53, 105
AluBI AGCT 8 cut(s) 126, 175, 250, 260, 406, 605, 676, 772
AluI AGCT 8 cut(s) 126, 175, 250, 260, 406, 605, 676, 772
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 247, 464
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 297, 466
AspS9I GGNCC 2 cut(s) 714, 764
AsuHPI GGTGA 1 cut(s) 593
AvaII GGWCC 2 cut(s) 714, 764
BaeGI GKGCMC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 313, 746
BceAI ACGGC 1 cut(s) 670
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 55, 107
BclI TGATCA 1 cut(s) 163
BcoDI GTCTC 2 cut(s) 247, 464
BfoI RGCGCY 1 cut(s) 298
BfrI CTTAAG 1 cut(s) 121
BisI GCNGC 6 cut(s) 18, 310, 343, 518, 553, 577
BlpI GCTNAGC 2 cut(s) 256, 773
BlsI GCNGC 6 cut(s) 19, 311, 344, 519, 554, 578
Bme1390I CCNGG 2 cut(s) 55, 107
Bme18I GGWCC 2 cut(s) 714, 764
BmgT120I GGNCC 2 cut(s) 714, 764
BmiI GGNNCC 2 cut(s) 516, 715
BmrFI CCNGG 2 cut(s) 55, 107
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 179
BpmI CTGGAG 5 cut(s) 37, 488, 530, 629, 780
Bpu10I CCTNAGC 1 cut(s) 768
Bpu1102I GCTNAGC 2 cut(s) 256, 773
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 84, 106, 304, 343, 717
BsaXI ACNNNNNCTCC 4 cut(s) 506, 536, 665, 695
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 2 cut(s) 74, 185
BseBI CCWGG 2 cut(s) 55, 107
BseDI CCNNGG 5 cut(s) 84, 106, 304, 343, 717
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 5 cut(s) 190, 270, 305, 717, 759
BseNI ACTGG 2 cut(s) 74, 185
BseRI GAGGAG 2 cut(s) 527, 599
BseSI GKGCMC 1 cut(s) 326
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 29
Bsh1236I CGCG 2 cut(s) 345, 464
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 1 cut(s) 309
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 1 cut(s) 641
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 2 cut(s) 247, 464
BsmBI CGTCTC 1 cut(s) 464
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 177, 326
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 2 cut(s) 163, 399
Bsp1720I GCTNAGC 2 cut(s) 256, 773
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 5 cut(s) 189, 269, 304, 718, 760
BspFNI CGCG 2 cut(s) 345, 464
BspLI GGNNCC 2 cut(s) 516, 715
BspTI CTTAAG 1 cut(s) 121
BsrBI CCGCTC 2 cut(s) 255, 288
BsrFI RCCGGY 1 cut(s) 640
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 2 cut(s) 74, 185
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 5 cut(s) 84, 106, 304, 343, 717
BssMI GATC 2 cut(s) 163, 399
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 55, 107
Bst4CI ACNGT 1 cut(s) 268
BstACI GRCGYC 1 cut(s) 413
BstAFI CTTAAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 203
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 2 cut(s) 408, 642
BstDEI CTNAG 8 cut(s) 176, 246, 256, 291, 726, 768, 773, 788
BstDSI CCRYGG 1 cut(s) 343
BstFNI CGCG 2 cut(s) 345, 464
BstH2I RGCGCY 1 cut(s) 298
BstHHI GCGC 2 cut(s) 297, 466
BstKTI GATC 2 cut(s) 166, 402
BstMAI GTCTC 2 cut(s) 247, 464
BstMBI GATC 2 cut(s) 163, 399
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 7 cut(s) 203, 294, 342, 412, 463, 514, 611
BstNI CCWGG 2 cut(s) 55, 107
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 53, 105
BstSLI GKGCMC 1 cut(s) 326
BstUI CGCG 2 cut(s) 345, 464
BstV1I GCAGC 1 cut(s) 29
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 408, 642
CfoI GCGC 2 cut(s) 297, 466
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 714, 764
Cfr42I CCGCGG 1 cut(s) 346
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 61, 682
CviAII CATG 5 cut(s) 13, 194, 350, 685, 739
CviQI GTAC 2 cut(s) 61, 682
DdeI CTNAG 8 cut(s) 176, 246, 256, 291, 726, 768, 773, 788
DpnI GATC 2 cut(s) 165, 401
DpnII GATC 2 cut(s) 163, 399
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
EciI GGCGGA 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 714, 764
Eco52I CGGCCG 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 53, 105
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 717
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 5 cut(s) 16, 197, 353, 688, 742
FaiI YATR 7 cut(s) 14, 195, 351, 563, 680, 686, 740
FatI CATG 5 cut(s) 12, 193, 349, 684, 738
FauI CCCGC 4 cut(s) 450, 561, 622, 703
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 18, 310, 343, 518, 553, 577
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 6 cut(s) 18, 310, 343, 518, 553, 577
GlaI GCGC 2 cut(s) 296, 465
GluI GCNGC 6 cut(s) 18, 310, 343, 518, 553, 577
GsuI CTGGAG 5 cut(s) 37, 488, 530, 629, 780
HaeII RGCGCY 1 cut(s) 298
HaeIII GGCC 1 cut(s) 309
HapII CCGG 1 cut(s) 641
HgaI GACGC 1 cut(s) 421
HhaI GCGC 2 cut(s) 297, 466
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 5 cut(s) 16, 197, 353, 688, 742
Hin6I GCGC 2 cut(s) 295, 464
HinP1I GCGC 2 cut(s) 295, 464
HinfI GANTC 3 cut(s) 35, 180, 722
HpaII CCGG 1 cut(s) 641
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 5 cut(s) 85, 142, 154, 179, 727
Hpy188III TCNNGA 7 cut(s) 32, 167, 368, 397, 608, 759, 767
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 5 cut(s) 104, 173, 415, 421, 656
HpyAV CCTTC 2 cut(s) 403, 586
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 3 cut(s) 197, 206, 537
HpyF10VI GCNNNNNNNGC 7 cut(s) 203, 294, 342, 412, 463, 514, 611
HpyF3I CTNAG 8 cut(s) 176, 246, 256, 291, 726, 768, 773, 788
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 5 cut(s) 16, 197, 353, 688, 742
HspAI GCGC 2 cut(s) 295, 464
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 1 cut(s) 163
KspI CCGCGG 1 cut(s) 346
Kzo9I GATC 2 cut(s) 163, 399
LmnI GCTCC 3 cut(s) 255, 514, 673
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 647
MalI GATC 2 cut(s) 165, 401
MbiI CCGCTC 2 cut(s) 255, 288
MboI GATC 2 cut(s) 163, 399
MboII GAAGA 2 cut(s) 490, 556
MhlI GDGCHC 2 cut(s) 177, 326
MlyI GAGTC 2 cut(s) 29, 716
MroNI GCCGGC 1 cut(s) 640
MseI TTAA 2 cut(s) 122, 233
MspA1I CMGCKG 2 cut(s) 345, 520
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 1 cut(s) 641
MspR9I CCNGG 2 cut(s) 55, 107
MvaI CCWGG 2 cut(s) 55, 107
MvnI CGCG 2 cut(s) 345, 464
MwoI GCNNNNNNNGC 7 cut(s) 203, 294, 342, 412, 463, 514, 611
NaeI GCCGGC 1 cut(s) 642
NdeII GATC 2 cut(s) 163, 399
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 5 cut(s) 16, 197, 353, 688, 742
NlaIV GGNNCC 2 cut(s) 516, 715
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 647
NspI RCATGY 1 cut(s) 353
PciI ACATGT 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 53
PkrI GCNGC 6 cut(s) 19, 311, 344, 519, 554, 578
PleI GAGTC 2 cut(s) 29, 716
PpsI GAGTC 2 cut(s) 29, 716
PpuMI RGGWCCY 1 cut(s) 764
PscI ACATGT 1 cut(s) 349
Psp124BI GAGCTC 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 2 cut(s) 53, 105
PspGI CCWGG 2 cut(s) 53, 105
PspN4I GGNNCC 2 cut(s) 516, 715
PspPI GGNCC 2 cut(s) 714, 764
PspPPI RGGWCCY 1 cut(s) 764
RsaI GTAC 2 cut(s) 62, 683
RsaNI GTAC 2 cut(s) 61, 682
SacI GAGCTC 1 cut(s) 177
SacII CCGCGG 1 cut(s) 346
SaqAI TTAA 2 cut(s) 122, 233
SatI GCNGC 6 cut(s) 18, 310, 343, 518, 553, 577
Sau3AI GATC 2 cut(s) 163, 399
Sau96I GGNCC 2 cut(s) 714, 764
SchI GAGTC 2 cut(s) 29, 716
ScrFI CCNGG 2 cut(s) 55, 107
SduI GDGCHC 2 cut(s) 177, 326
SfaNI GCATC 1 cut(s) 452
Sfr303I CCGCGG 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 346
SinI GGWCC 2 cut(s) 714, 764
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 53, 105
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 1 cut(s) 402
TatI WGTACW 1 cut(s) 681
TauI GCSGC 5 cut(s) 312, 345, 520, 555, 579
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 2 cut(s) 122, 233
Tru9I TTAA 2 cut(s) 122, 233
TscAI CASTG 1 cut(s) 74
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 3 cut(s) 344, 578, 758
TspGWI ACGGA 1 cut(s) 488
TspRI CASTG 1 cut(s) 74
Vha464I CTTAAG 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 714, 764
XceI RCATGY 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.