FvH4_4g28270

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013999
Physical Location & Seq
Reverse (-)
28954151 .. 28954951
801 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28270.t1

Sequence Viewer

Length: 801 bp
ATGAGGACAATCATGGCAGCCAAGACCCCTCATGACTCCTCCTTCTCATTTTCCAGAAGGTACTTCTGGAAAAAGAAGGTCGACGATGATGAAGACGACGATCACGAAGTCCTCAACTTCAGCTCCTACTCACACTTCTCCGAGGAAGAGAAAGAAGAGGAGCTGGCCCGGAACATCCATATGTCAACAGCAACCGATCAGATTGTTGCATCAGTCTTACCTGAAGTGCCACGCAAAAAATACTCACTGGTCTCAGTCTCTAAGCTCCGGTCAGCTCTTTTCGGCAAGAGCCGTGCTGGCTCTCGCTCCGGTTTAATGGGCACGCTTTTCGGGTATCGCCGCGGCCATGTTCACTTGGCATTTCAGGAGGATCCCAAGCTGAACCCTACGTTTCTCATCGAGCTGGCTACGCCAACTAGTGTTCTGGTTCGAGAAATGGCTTCAGGGTTGGTCAGAATTGCGTTGGAGTGTGAGAAGAAGACTGATCATAAGAAGAAGGGGCTGAAGTTGCTGGAGGAGCCGCTGTGGAGGACTTACTGCAATGGCAAGAAGTGTGGTTTCGCTATGAGGCGAGATTGTGGTCCAGAGGAGTGGAAGGTGCTGAAAGCCCTGGAGCCGGTTTCAATGGGGGCTGGGGTTTTACCTGGGAATGAAGATGGAGCAGGATCTGAAGGTGAGCTCATGTACATGAGAGCCAAGTTTGAGAGAGTTGTGGGGTCTAAGGACTCAGAGGCCTTCTACATGATGAACCCAGATGGTTCTGCTGGTCCTGAACTCAGTGTTTACTTGCTTAGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

267

Amino Acids

29.94

Weight (kDa)

7.68

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 106 - 265 2.5e-73 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013999)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25640 AT3G25640
fragaria_vesca FvH4_4g28270
malus_domestica MD13G1048700.v1.1 MD16G1050000.v1.1
prunus_persica Prupe.1G303400_v2.0.a1
pyrus_communis pycom16g04380
rosa_chinensis RchiOBHm_Chr4g0436141
rosa_laevigata RLG00000006537
rosa_multiflora Rmu_sc0022632.1_g000001
rosa_roxburghii Rroxscaffold_5G00377240
rosa_rugosa Rorug04G0292200
rosa_samantha Rh4AG346200 Rh4BG355300 Rh4CG369900 Rh4DG348900
rosa_wichuraiana Rw4G030270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AccII CGCG 1 cut(s) 342
AciI CCGC 3 cut(s) 340, 342, 521
AclWI GGATC 3 cut(s) 365, 378, 673
AcoI YGGCCR 1 cut(s) 343
AcuI CTGAAG 5 cut(s) 103, 243, 426, 524, 690
AfaI GTAC 2 cut(s) 62, 686
AfiI CCNNNNNNNGG 1 cut(s) 616
AgsI TTSAA 1 cut(s) 624
AhlI ACTAGT 1 cut(s) 416
AjnI CCWGG 2 cut(s) 609, 643
AluBI AGCT 7 cut(s) 123, 163, 265, 275, 379, 403, 679
AluI AGCT 7 cut(s) 123, 163, 265, 275, 379, 403, 679
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 2 cut(s) 256, 262
AlwI GGATC 3 cut(s) 365, 378, 673
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 3 cut(s) 165, 343, 732
ApeKI GCWGC 1 cut(s) 17
AspS9I GGNCC 3 cut(s) 166, 581, 767
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 686
AvaII GGWCC 2 cut(s) 581, 767
BaeGI GKGCMC 1 cut(s) 323
BamHI GGATCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 681
BbsI GAAGAC 2 cut(s) 99, 485
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 1 cut(s) 29
BccI CCATC 2 cut(s) 650, 749
BceAI ACGGC 1 cut(s) 276
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 611, 645
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 256, 262
BcuI ACTAGT 1 cut(s) 416
BfaI CTAG 2 cut(s) 417, 799
BglI GCCNNNNNGGC 1 cut(s) 297
BisI GCNGC 4 cut(s) 18, 340, 343, 521
BlsI GCNGC 4 cut(s) 19, 341, 344, 522
Bme1390I CCNGG 3 cut(s) 169, 611, 645
Bme18I GGWCC 2 cut(s) 581, 767
BmgT120I GGNCC 3 cut(s) 166, 581, 767
BmiI GGNNCC 3 cut(s) 372, 519, 615
BmrFI CCNGG 3 cut(s) 169, 611, 645
BmsI GCATC 1 cut(s) 218
BpiI GAAGAC 2 cut(s) 99, 485
BpmI CTGGAG 2 cut(s) 533, 632
BpuMI CCSGG 1 cut(s) 169
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 141, 340, 609, 644
BsaWI WCCGGW 2 cut(s) 267, 308
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 509, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 252
Bse3DI GCAATG 1 cut(s) 547
BseBI CCWGG 2 cut(s) 611, 645
BseDI CCNNGG 4 cut(s) 141, 340, 609, 644
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 3 cut(s) 267, 741, 790
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 4 cut(s) 28, 173, 530, 602
BseSI GKGCMC 1 cut(s) 323
BseXI GCAGC 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 632
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 3 cut(s) 167, 345, 734
BsiHKAI GWGCWC 1 cut(s) 681
BsiSI CCGG 4 cut(s) 169, 268, 309, 617
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 2 cut(s) 256, 262
BsnI GGCC 3 cut(s) 167, 345, 734
Bso31I GGTCTC 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 323, 681
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 5 cut(s) 100, 196, 370, 484, 665
BspACI CCGC 3 cut(s) 340, 342, 521
BspANI GGCC 3 cut(s) 167, 345, 734
BspCNI CTCAG 3 cut(s) 266, 740, 789
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 3 cut(s) 372, 519, 615
BspPI GGATC 3 cut(s) 365, 378, 673
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 616
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 1 cut(s) 252
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 4 cut(s) 141, 340, 609, 644
BssMI GATC 5 cut(s) 100, 196, 370, 484, 665
Bst2UI CCWGG 2 cut(s) 611, 645
Bst6I CTCTTC 2 cut(s) 141, 150
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 4 cut(s) 165, 298, 323, 405
BstDEI CTNAG 6 cut(s) 253, 261, 720, 727, 776, 791
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 1 cut(s) 342
BstKTI GATC 5 cut(s) 103, 199, 373, 487, 668
BstMAI GTCTC 2 cut(s) 256, 262
BstMBI GATC 5 cut(s) 100, 196, 370, 484, 665
BstMWI GCNNNNNNNGC 4 cut(s) 297, 409, 508, 517
BstNI CCWGG 2 cut(s) 611, 645
BstSCI CCNGG 3 cut(s) 167, 609, 643
BstSLI GKGCMC 1 cut(s) 323
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 2 cut(s) 99, 485
BstX2I RGATCY 2 cut(s) 370, 665
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 2 cut(s) 370, 665
BsuRI GGCC 3 cut(s) 167, 345, 734
BtgI CCRYGG 1 cut(s) 340
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 2 cut(s) 245, 784
Cac8I GCNNGC 4 cut(s) 165, 298, 323, 405
CaiI CAGNNNCTG 1 cut(s) 668
CciI TCATGA 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 3 cut(s) 166, 581, 767
Cfr42I CCGCGG 1 cut(s) 343
Csp6I GTAC 2 cut(s) 61, 685
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 6 cut(s) 13, 32, 347, 682, 688, 742
CviQI GTAC 2 cut(s) 61, 685
DdeI CTNAG 6 cut(s) 253, 261, 720, 727, 776, 791
DpnI GATC 5 cut(s) 102, 198, 372, 486, 667
DpnII GATC 5 cut(s) 100, 196, 370, 484, 665
EaeI YGGCCR 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 141, 150
EarI CTCTTC 2 cut(s) 141, 150
Ecl136II GAGCTC 1 cut(s) 679
Eco147I AGGCCT 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 681
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 581, 767
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 5 cut(s) 103, 243, 426, 524, 690
EcoICRI GAGCTC 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 609, 643
EcoT38I GRGCYC 1 cut(s) 681
FaeI CATG 6 cut(s) 16, 35, 350, 685, 691, 745
FatI CATG 6 cut(s) 12, 31, 346, 681, 687, 741
FauNDI CATATG 1 cut(s) 180
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 18, 340, 343, 521
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 4 cut(s) 18, 340, 343, 521
FspBI CTAG 2 cut(s) 417, 799
GluI GCNGC 4 cut(s) 18, 340, 343, 521
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 533, 632
HaeIII GGCC 3 cut(s) 167, 345, 734
HapII CCGG 4 cut(s) 169, 268, 309, 617
Hin1II CATG 6 cut(s) 16, 35, 350, 685, 691, 745
HincII GTYRAC 2 cut(s) 82, 186
HindII GTYRAC 2 cut(s) 82, 186
HinfI GANTC 3 cut(s) 35, 725, 795
HpaII CCGG 4 cut(s) 169, 268, 309, 617
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 4 cut(s) 82, 186, 352, 784
Hpy188I TCNGA 5 cut(s) 142, 201, 455, 670, 730
Hpy188III TCNNGA 8 cut(s) 32, 54, 67, 104, 365, 431, 584, 770
Hpy8I GTNNAC 4 cut(s) 82, 186, 352, 784
Hpy99I CGWCG 2 cut(s) 86, 101
HpyAV CCTTC 7 cut(s) 51, 52, 70, 490, 589, 665, 745
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 2 cut(s) 209, 540
HpyF10VI GCNNNNNNNGC 4 cut(s) 297, 409, 508, 517
HpyF3I CTNAG 6 cut(s) 253, 261, 720, 727, 776, 791
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 6 cut(s) 16, 35, 350, 685, 691, 745
Ksp22I TGATCA 1 cut(s) 484
KspI CCGCGG 1 cut(s) 343
Kzo9I GATC 5 cut(s) 100, 196, 370, 484, 665
LmnI GCTCC 7 cut(s) 128, 160, 270, 311, 517, 613, 659
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 218
MaeI CTAG 2 cut(s) 417, 799
MaeII ACGT 1 cut(s) 389
MalI GATC 5 cut(s) 102, 198, 372, 486, 667
MboI GATC 5 cut(s) 100, 196, 370, 484, 665
MboII GAAGA 7 cut(s) 104, 158, 167, 487, 490, 505, 665
MflI RGATCY 2 cut(s) 370, 665
MhlI GDGCHC 2 cut(s) 323, 681
MluCI AATT 1 cut(s) 456
MlyI GAGTC 2 cut(s) 29, 719
MmeI TCCRAC 1 cut(s) 444
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 2 cut(s) 179, 686
MspA1I CMGCKG 2 cut(s) 342, 523
MspI CCGG 4 cut(s) 169, 268, 309, 617
MspR9I CCNGG 3 cut(s) 169, 611, 645
MvaI CCWGG 2 cut(s) 611, 645
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 4 cut(s) 297, 409, 508, 517
NciI CCSGG 1 cut(s) 169
NdeI CATATG 1 cut(s) 180
NdeII GATC 5 cut(s) 100, 196, 370, 484, 665
NlaIII CATG 6 cut(s) 16, 35, 350, 685, 691, 745
NlaIV GGNNCC 3 cut(s) 372, 519, 615
PagI TCATGA 1 cut(s) 31
PceI AGGCCT 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PkrI GCNGC 4 cut(s) 19, 341, 344, 522
PleI GAGTC 2 cut(s) 29, 719
PpsI GAGTC 2 cut(s) 29, 719
Psp124BI GAGCTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 609, 643
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 2 cut(s) 609, 643
PspN4I GGNNCC 3 cut(s) 372, 519, 615
PspPI GGNCC 3 cut(s) 166, 581, 767
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 2 cut(s) 370, 665
RsaI GTAC 2 cut(s) 62, 686
RsaNI GTAC 2 cut(s) 61, 685
RseI CAYNNNNRTG 2 cut(s) 179, 686
SacI GAGCTC 1 cut(s) 681
SacII CCGCGG 1 cut(s) 343
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 4 cut(s) 18, 340, 343, 521
Sau3AI GATC 5 cut(s) 100, 196, 370, 484, 665
Sau96I GGNCC 3 cut(s) 166, 581, 767
SchI GAGTC 2 cut(s) 29, 719
ScrFI CCNGG 3 cut(s) 169, 611, 645
SduI GDGCHC 2 cut(s) 323, 681
SfaNI GCATC 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 343
SgrBI CCGCGG 1 cut(s) 343
SinI GGWCC 2 cut(s) 581, 767
SmiMI CAYNNNNRTG 2 cut(s) 179, 686
SpeI ACTAGT 1 cut(s) 416
Sse9I AATT 1 cut(s) 456
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 3 cut(s) 340, 342, 521
SspMI CTAG 2 cut(s) 417, 799
SstI GAGCTC 1 cut(s) 681
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 3 cut(s) 167, 609, 643
TaiI ACGT 1 cut(s) 392
TaqI TCGA 3 cut(s) 81, 399, 430
TasI AATT 1 cut(s) 456
TatI WGTACW 1 cut(s) 684
TauI GCSGC 3 cut(s) 342, 345, 523
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 2 cut(s) 252, 784
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 3 cut(s) 105, 666, 761
TspRI CASTG 2 cut(s) 252, 784
VpaK11BI GGWCC 2 cut(s) 581, 767
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 2 cut(s) 417, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.